CN109632752A - The method and detector of various metals ion are identified by fluorescent carbon point - Google Patents
The method and detector of various metals ion are identified by fluorescent carbon point Download PDFInfo
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Abstract
The invention discloses a kind of methods for identifying various metals ion by fluorescent carbon point, the following steps are included: providing N kind fluorescent carbon point (N >=1), the aqueous metallic ions of N group isoconcentration are provided, the corresponding aqueous solution that a kind of fluorescent carbon point is added into every group of aqueous metallic ions；Fluorescence intensity level of the solution under each fluorescent emission channel before and after aqueous metallic ions is added in detection, carries out principal component analysis to obtained fluorescence intensity change data, establishes standard principal component analysis figure；Carrying out fluorescence intensity detection and principal component analysis to solution to be measured after the same method can recognize the detection substrate in solution to be measured according to position of the obtained numerical value in standard principal component analysis figure.The method for identifying various metals ion by fluorescent carbon point of the invention, realizes the detection and identification to various metals ion, to cope with the detection and analysis of polynary substrate samples under complex environment in practical application.
Description
Technical field
The present invention relates to organic carbon material application and detection method fields, and in particular to a kind of more by fluorescent carbon point identification
The method of metal ion species and the detector for implementing the method.
Background technique
For natural environment and organic-biological body, certain metal cations or anion and biological micromolecule are often play
Multiple key player.Some guest molecules there are of great advantage, and some human health and ambient enviroment can then be generated it is tight
It endangers again.For example, Fe (III), Cu (II) are widely present in enzyme and protein, and right as microelement important in vivo
Cell metabolism generates important function, and content is too low to cause human body uncomfortable, and too high levels can then become toxic element destruction
Organism simultaneously induces it that lesion occurs.With a large amount of discharges of industrial wastewater, the heavy metals such as Hg (II), Pb (II), Cd (II) from
Son more and more enters in natural environment.These heavy metal ion are easily absorbed by plant and organism, are enriched with, and finally will
It constitutes a serious threat to environment and human security.Therefore, efficient, sensitive detection means is researched and developed to realize to Metals in Environments
The detection of ion is particularly important.Traditional detection means generallys use large-scale instrument and equipment, but due to its is complicated for operation,
Testing the disadvantages of cumbersome, time-consuming hinders it and is widely used.Optochemical sensor is as a kind of cheap, easy to operate, high sensitivity
The detection means of (generally up to ppb grades) is concerned.
Carbon dots are as carbon nanomaterial emerging in recent years, and because having, hyperfluorescence, hypotoxicity, wavelength of fluorescence is adjustable, unglazed
The characteristics such as flashing make it be used widely in fields such as ion detections.Guo etc. (carbon, 2013,52,583-589) passes through
Sodium citrate and ammonium hydrogen carbonate hydrothermal synthesis of carbon point, and it is used for Hg2+Detection.Dong etc. (J.Mater.Chem.B,
Carbon dots 2014,2,6995-6999) are synthesized and to Hg with one step microwave method of ethylenediamine by citric acid2+It is detected.Qu etc.
(Chem.Eur.J.2013,19,7243) dopamine is handled by hydro-thermal method to obtain to Fe3+There are the carbon dots of specific recognition function.
However application of the above-mentioned carbon dots in metal ion detection, it has focused largely on to specific a certain or two kinds of ions
Detection, and distinguish also seldom with the research that detects while to different kinds of ions.And in nature and production activity, our faces
Pair be polynary substrate sample and complex environment.The chemical detection of traditional " one-to-one " based on " lock-key " type,
It is difficult to cope with to the requirements at the higher level of complex system detection in actual life, therefore, the more substrates detection for studying carbon dots seems especially
It is important.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of methods for identifying various metals ion by fluorescent carbon point, should
Method utilizes otherness phenomenon of the carbon dots fluorescent emission intensity to the fluorescence response of different metal ions, reality under different excitation wavelengths
Now to the detection and identification of various metals ion, thus cope in practical application under complex environment the detection of polynary substrate samples with
Analysis.
In order to solve the above-mentioned technical problem, one aspect of the present invention provide it is a kind of by fluorescent carbon point identification various metals from
The method of son, comprising:
It provides N kind fluorescent carbon point (N >=1), the N kind fluorescent carbon point has multiple fluorescent emission channels in total；
The aqueous metallic ions of N group isoconcentration are provided, correspondence is added described in one kind into every group of aqueous metallic ions
The aqueous solution of fluorescent carbon point；Wherein, every group of aqueous metallic ions are selected from Al3+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Hg2+,
Cd2+, Ca2+, Mg2+、Ba2+、Na+、Cr2+、Ni2+、Mn2+、Ce3+、Pb2+At least one of aqueous solution；
Fluorescence intensity level of the solution under each fluorescent emission channel before and after aqueous metallic ions is added in detection, to obtaining
Fluorescence intensity change data carry out principal component analysis, establish standard principal component analysis figure；And
N group solution to be measured is provided, a kind of corresponding aqueous solution that fluorescent carbon point is added, detection into every group of solution to be measured
Fluorescence intensity level of the solution front and back to be measured solution under each fluorescent emission channel is added, to obtained fluorescence intensity change data
Carry out principal component analysis can recognize in solution to be measured according to position of the obtained numerical value in standard principal component analysis figure
Detection substrate.
Further, at least one fluorescent carbon point is a unimodal fluorescent carbon point and a bimodal fluorescent carbon point.
Further, the aqueous solution of the unimodal fluorescent carbon point the preparation method comprises the following steps: carbon source is taken to be dissolved in ethyl alcohol, obtain anti-
Answer presoma；Obtained precursors are subjected to 2~12h of hydro-thermal reaction at 120~240 DEG C, obtain unimodal fluorescent carbon point
Aqueous solution；
The aqueous solution of the bimodal fluorescent carbon point the preparation method comprises the following steps: carbon source is taken to be dissolved in ethyl alcohol, reproducibility nitrogen source is added,
Obtain precursors；Obtained precursors are subjected to 2~12h of hydro-thermal reaction at 120~240 DEG C, it is glimmering to obtain multimodal
Light carbon dots aqueous solution.
Further, the carbon source is selected from 5- amino isophthalic acid, 2- amino isophthalic acid, 4- amino isophthalic diformazan
Acid, 2- amino terephthalic acid (TPA), 2,5- diamino terephthalic acid (TPA), ortho-aminobenzoic acid, gavaculine, p-aminophenyl first
One of acid, 3- aminophthalic acid and 4- aminophthalic acid.
Further, the reproducibility nitrogen source is selected from ethylenediamine, ammonium hydroxide, ethanol amine, propylamine, 1,2- propane diamine, 1,3- third
One of diamines, hydrazine hydrate, triethylamine and diethylamine.
Further, the molar ratio of the carbon source and reproducibility nitrogen source is 1:1~1:10.
Further, further include the steps that post-processing obtained fluorescent carbon point aqueous solution；The post-processing are as follows: will
Fluorescent carbon point aqueous solution is centrifuged to remove large granular impurity, then carries out rotary evaporation, obtained powdered fluorescent carbon point is spent
Ionized water dissolution.
Further, the concentration of the fluorescent carbon point aqueous solution is 0.001~0.01g/L, the aqueous metallic ions
Concentration be 0.5~5mM.
Further, the volume ratio of the aqueous solution of fluorescent carbon point and aqueous metallic ions is 1:1~1:10.
According to the another aspect of the application, a kind of detector is provided, it is more which implements any of the above-described kind of identification
The method of metal ion species.
Beneficial effects of the present invention:
1, the present invention passes through solvent structure carbon dots, easy to operate, is readily synthesized, convenient for functionization.
2, the present invention passes through a variety of of the combination by being combined multiple carbon dots emission peaks under different excitation wavelengths
To the otherness of each metal ion response under emission state, the detection and analysis to various metals ion are realized, reality can be coped with
In the application of border under complex environment polynary substrate samples detection and analysis.This method is only achieved that with 1-2 kind carbon dots to most
Detection and discrimination, avoid the cumbersome technique of synthesis while 17 kinds of metal cations, simplify detection process, have outstanding ring
Border friendly and chemical economy.
Detailed description of the invention
Fig. 1 is the schematic diagram of metal ion and fluorescent carbon point interaction in the present invention；
Fig. 2 is the TEM figure of the unimodal fluorescent carbon point CD1 of embodiment 1；
The fluorescence excitation and launching light spectrogram that Fig. 3 is the unimodal fluorescent carbon point CD1 of embodiment 1；
The fluorescence excitation and launching light spectrogram that Fig. 4 is the bimodal fluorescent carbon point CD2 of embodiment 1；
Fig. 5 is 2 kinds of fluorescent carbon points fluorescence response figure with different metal cation respectively in embodiment 1；
Fig. 6 is the principal component analysis figure of each metal ion in embodiment 1.
Specific embodiment
The present invention will be further explained below with reference to the attached drawings and specific examples, so that those skilled in the art can be with
It more fully understands the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
As stated in the background art, the chemical detection of traditional " one-to-one " based on " lock-key " type, it is difficult to reply reality
To the requirements at the higher level of complex system detection in life, therefore, the more substrates detection for studying carbon dots is particularly important.In order to solve
This technical problem, this application provides a solution, can identify various metals ion by fluorescent carbon point.
This method specifically includes the following steps:
It provides N kind fluorescent carbon point (N >=1), the N kind fluorescent carbon point has multiple fluorescent emission channels in total；
The aqueous metallic ions of N group isoconcentration are provided, correspondence is added described in one kind into every group of aqueous metallic ions
The aqueous solution of fluorescent carbon point；Wherein, every group of aqueous metallic ions are selected from Al3+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Hg2+,
Cd2+, Ca2+, Mg2+、Ba2+、Na+、Cr2+、Ni2+、Mn2+、Ce3+、Pb2+At least one of aqueous solution；
Fluorescence intensity level of the solution under each fluorescent emission channel before and after aqueous metallic ions is added in detection, to obtaining
Fluorescence intensity change data carry out principal component analysis, establish standard principal component analysis figure；And
N group solution to be measured is provided, a kind of corresponding aqueous solution that fluorescent carbon point is added, detection into every group of solution to be measured
Fluorescence intensity level of the solution front and back to be measured solution under each fluorescent emission channel is added, to obtained fluorescence intensity change data
Carry out principal component analysis can recognize in solution to be measured according to position of the obtained numerical value in standard principal component analysis figure
Detection substrate.
N kind fluorescent carbon point in the application has multiple fluorescent emission channels (or referred to as fluorescence emission peak) in total, excellent
Selection of land has at least three fluorescent emission channel.Its principle is: by multiple fluorescence emission peaks to different metal ions responsiveness
Differentiation phenomenon realizes detection and identification to various metals ion.
In a kind of exemplary embodiment of the application, the N kind fluorescent carbon point is that a unimodal fluorescent carbon point and one are bimodal glimmering
Light carbon dots have 3 fluorescent emission channels in total.Preferably, the partial size of the unimodal fluorescent carbon point and bimodal fluorescent carbon point that use
For 1~10nm.
Present invention also provides a kind of sides of preparation of the aqueous solution of the aqueous solution of unimodal fluorescent carbon point and bimodal fluorescent carbon point
Method.Wherein, unimodal fluorescent carbon point aqueous solution the preparation method comprises the following steps: carbon source is taken to be dissolved in ethyl alcohol, obtain precursors；It will obtain
Precursors 2~12h of hydro-thermal reaction is carried out at 120~240 DEG C, obtain the unimodal fluorescent carbon point aqueous solution of brown color.
The aqueous solution of the bimodal fluorescent carbon point the preparation method comprises the following steps: carbon source is taken to be dissolved in ethyl alcohol, reproducibility nitrogen source is added,
Obtain precursors；Obtained precursors are subjected to 2~12h of hydro-thermal reaction at 120~240 DEG C, obtain brown color
Multimodal fluorescent carbon point aqueous solution.
In above-mentioned preparation method, the carbon source is the molecule containing phenyl ring, amino and carboxyl structure.Preferably, the carbon
Source is selected from 5- amino isophthalic acid, 2- amino isophthalic acid, 4- amino isophthalic acid, 2- amino terephthalic acid (TPA), 2,
5- diamino terephthalic acid (TPA), ortho-aminobenzoic acid, gavaculine, p-aminobenzoic acid, 3- aminophthalic acid and
One of 4- aminophthalic acid.The reproducibility nitrogen source is selected from ethylenediamine, ammonium hydroxide, ethanol amine, propylamine, 1,2- the third two
One of amine, 1,3- propane diamine, hydrazine hydrate, triethylamine and diethylamine.Preferably, the molar ratio of carbon source and reproducibility nitrogen source is
1:1~1:10.
The impurity content in fluorescent carbon point in order to reduce preparation improves purity, it is preferable that further include unimodal glimmering to obtaining
The step of light carbon dots solution and multimodal fluorescent carbon point solution are post-processed.The post-processing can be with are as follows: glimmering by what is be prepared
Light carbon dots solution is centrifuged, to remove the large granular impurity in solution；Then head product solution is subjected to rotary evaporation, obtained
Powder deionized water dissolving.
In the application, the concentration of the fluorescent carbon point aqueous solution is preferably 0.001~0.01g/L, more preferably 0.005g/
L.The concentration of the aqueous metallic ions is preferably 0.5~5mM.The fluorescent carbon point aqueous solution and aqueous metallic ions
Volume ratio is preferably 1:1~1:10.
In the application, in the measurement process of fluorescence intensity, the excitation wavelength used is 265~415nm.Preferably, single
The excitation wavelength of peak fluorescent carbon point is near 315nm, and the wave peak-peak of fluorescence emission peak is near 415nm.Preferably, bimodal
When the excitation wavelength of fluorescent carbon point is near 315nm, fluorescence emission peak is near 415nm；When excitation wavelength is near 390nm,
Fluorescence emission peak is near 510nm.
In the another embodiment of the application, provide it is a kind of can be to various metals ion is detected and is identified
Detector, the detector implement any of the above-described kind of method for identifying various metals ion by fluorescent carbon point.
Below with reference to embodiment, the scheme of the application is further illustrated.
Embodiment 1
(1) it weighs 0.3g 5- amino isophthalic acid to be placed in a beaker, 30mL ethyl alcohol is added and stirs completely molten to solid
Solution is not added nitrogen source by parallel laboratory test and 1mL ethylenediamine is added, obtains 2 kinds of different presomas；Evenly dispersed presoma is molten
Liquid is transferred to the hydrothermal reaction kettle of polytetrafluoroethylliner liner respectively, is reacted in an oven, reaction temperature: 180 DEG C, when reaction
Between: 10h obtains the solution of 2 kinds of different carbon dots；Obtained solution is centrifuged to remove large granular impurity；Later by head product
Solution carries out rotary evaporation, obtains after powder and with deionized water dissolving, for future use.
It by taking the unimodal fluorescent carbon point CD1 that ethylenediamine is not added as an example, is characterized using transmission electron microscope, particle size range is
2nm~5nm (referring to attached drawing 2)；The fluorescence emission peak of fluorescent carbon point CD1 is 415nm (referring to attached drawing 3)；Reacting ethylenediamine is added to obtain
To bimodal fluorescent carbon point CD2 have 2 fluorescence emission peaks under different excitations, when excitation wavelength is 315nm, fluorescence emission peak
For 415nm, when excitation wavelength is 390nm, fluorescence emission peak is 510nm (referring to attached drawing 4).
(2) fluorescent carbon point aqueous solution is added separately in the identical each aqueous metallic ions of a certain amount of concentration, is tested
The fluorescence intensity of front and back solution is added；The variation ratio of fluorescence before and after being added to metal ion is calculated, and with different metal ions
For abscissa, the variation ratio that metal ion front and back fluorescence intensity is added is ordinate, establishes carbon dots to different metal ions
The bar chart of fluorescence response (referring to attached drawing 5).The variation ratio of fluorescence before and after the addition metal ion being calculated is passed through into system
Meter learns principal component analysis (PCA), obtains grouping and component correlation result and the discriminance analysis of each detection substrate.To Al3+, Fe3 +, Co2+, Ni2+, Cu2+, Zn2+, Hg2+, Cd2+, Ca2+, Mg2+、Ba2+、Na+、Cr2+、Ni2+、Mn2+、Ce3+、Pb2+17 kinds of metal sun from
Son detection carries out to be distinguished and grouping by complete after 3 repetitions are tested respectively (referring to attached drawing 6).
Embodiment 2
(1) it weighs 0.5g 2- amino terephthalic acid (TPA) to be placed in a beaker, 50mL ethyl alcohol is added and stirs completely molten to solid
Solution is not added nitrogen source by parallel laboratory test and 1.2mL hydrazine hydrate is added, 2 kinds of different presomas obtained, by evenly dispersed presoma
Solution is transferred to the hydrothermal reaction kettle of polytetrafluoroethylliner liner respectively, is reacted in an oven, reaction temperature: 180 DEG C, reaction
Time: 8h obtains the solution of 2 kinds of different carbon dots；Obtained solution is centrifuged to remove large granular impurity；Later by head product
Solution carries out rotary evaporation, obtains after powder and with deionized water dissolving, for future use.
(2) fluorescent carbon point aqueous solution is separately added into the identical each aqueous metallic ions of a certain amount of concentration, test is mixed
Close the fluorescence intensity of solution；The variation ratio of fluorescence before and after metal ion is added is calculated, and using different metal ions as abscissa,
The variation ratio that fluorescence intensity before and after metal ion is added is ordinate, establishes carbon dots to the fluorescence responses of different metal ions
Bar chart；By the variation ratio that metal ion front and back fluorescence is added being calculated by statistics principal component analysis (PCA), obtain
The grouping of each detection substrate and component correlation result and discriminance analysis out.
Embodiment 3
(1) it weighs 0.3g 4- aminophthalic acid to be placed in a beaker, 30mL ethyl alcohol is added and stirs completely molten to solid
Solution is not added nitrogen source by parallel laboratory test and 0.8mL ethanol amine is added, 2 kinds of different presomas obtained, by evenly dispersed presoma
Solution is transferred to the hydrothermal reaction kettle of polytetrafluoroethylliner liner respectively, is reacted in an oven, reaction temperature: 200 DEG C, reaction
Time: 10h obtains the solution of 2 kinds of different carbon dots；Obtained solution is centrifuged to remove large granular impurity；Later by primiparity
Object solution carries out rotary evaporation, obtains after powder and with deionized water dissolving, for future use.
(2) fluorescent carbon point aqueous solution is separately added into the identical each aqueous metallic ions of a certain amount of concentration, test is mixed
Close the fluorescence intensity of solution；The variation ratio of fluorescence before and after metal ion is added is calculated, and using different metal ions as abscissa,
The variation ratio that fluorescence intensity before and after metal ion is added is ordinate, establishes carbon dots to the fluorescence responses of different metal ions
Bar chart；By the variation ratio that metal ion front and back fluorescence is added being calculated by statistics principal component analysis (PCA), obtain
The grouping of each detection substrate and component correlation result and discriminance analysis out.
Embodiment 4
(1) it weighs 0.5g p-aminobenzoic acid to be placed in a beaker, 50mL ethyl alcohol is added and stirring to solid is completely dissolved, leads to
It crosses parallel laboratory test nitrogen source is not added and 1.0mL1 is added, 2- propane diamine obtains 2 kinds of different presomas, by evenly dispersed presoma
Solution is transferred to the hydrothermal reaction kettle of polytetrafluoroethylliner liner respectively, is reacted in an oven, reaction temperature: 200 DEG C, reaction
Time: 6h obtains the solution of 2 kinds of different carbon dots；Obtained solution is centrifuged to remove large granular impurity；Later by head product
Solution carries out rotary evaporation, obtains after powder and with deionized water dissolving, for future use.
(2) fluorescent carbon point aqueous solution is separately added into the identical each aqueous metallic ions of a certain amount of concentration, test is mixed
Close the fluorescence intensity of solution；The variation ratio of fluorescence before and after metal ion is added is calculated, and using different metal ions as abscissa,
The variation ratio that fluorescence intensity before and after metal ion is added is ordinate, establishes carbon dots to the fluorescence responses of different metal ions
Bar chart；By the variation ratio that metal ion front and back fluorescence is added being calculated by statistics principal component analysis (PCA), obtain
The grouping of each detection substrate and component correlation result and discriminance analysis out.
Embodiment 5
(1) 0.4g 2 is weighed, 5- diamino terephthalic acid (TPA) is placed in a beaker, and 40mL ethyl alcohol is added and stirs complete to solid
Fully dissolved is not added nitrogen source by parallel laboratory test and 1.0mL triethylamine is added, 2 kinds of different presomas obtained, before evenly dispersed
The hydrothermal reaction kettle that liquid solution is transferred to polytetrafluoroethylliner liner respectively is driven, is reacted in an oven, reaction temperature: 200 DEG C,
Reaction time: 8h obtains the solution of 2 kinds of different carbon dots；Obtained solution is centrifuged to remove large granular impurity；It later will be first
Reaction mixture carries out rotary evaporation, obtains after powder and with deionized water dissolving, for future use.
(2) fluorescent carbon point aqueous solution is separately added into the identical each aqueous metallic ions of a certain amount of concentration, test is mixed
Close the fluorescence intensity of solution；The variation ratio of fluorescence before and after metal ion is added is calculated, and using different metal ions as abscissa,
The variation ratio that fluorescence intensity before and after metal ion is added is ordinate, establishes carbon dots to the fluorescence responses of different metal ions
Bar chart；By the variation ratio that metal ion front and back fluorescence is added being calculated by statistics principal component analysis (PCA), obtain
The grouping of each detection substrate and component correlation result and discriminance analysis out.
Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, protection model of the invention
It encloses without being limited thereto.Those skilled in the art's made equivalent substitute or transformation on the basis of the present invention, in the present invention
Protection scope within.Protection scope of the present invention is subject to claims.
Claims (10)
1. a kind of method for identifying various metals ion by fluorescent carbon point characterized by comprising
It provides N kind fluorescent carbon point (N >=1), the N kind fluorescent carbon point has multiple fluorescent emission channels in total；
The aqueous metallic ions of N group isoconcentration are provided, a kind of fluorescence is added in correspondence into every group of aqueous metallic ions
The aqueous solution of carbon dots；Wherein, every group of aqueous metallic ions are selected from Al3+, Fe3+, Co2+, Ni2+, Cu2+, Zn2+, Hg2+, Cd2+,
Ca2+, Mg2+、Ba2+、Na+、Cr2+、Ni2+、Mn2+、Ce3+、Pb2+At least one of aqueous solution；
Fluorescence intensity level of the solution under each fluorescent emission channel before and after aqueous metallic ions is added in detection, glimmering to what is obtained
Intensity variation data carry out principal component analysis, establish standard principal component analysis figure；And
N group solution to be measured is provided, a kind of corresponding aqueous solution that fluorescent carbon point is added, detection are added into every group of solution to be measured
Fluorescence intensity level of the solution under each fluorescent emission channel before and after solution to be measured, carries out obtained fluorescence intensity change data
Principal component analysis can recognize the detection in solution to be measured according to position of the obtained numerical value in standard principal component analysis figure
Substrate.
2. as described in claim 1 by fluorescent carbon point identification various metals ion method, which is characterized in that it is described at least
A kind of fluorescent carbon point is a unimodal fluorescent carbon point and a bimodal fluorescent carbon point.
3. the method as claimed in claim 2 for identifying various metals ion by fluorescent carbon point, which is characterized in that described unimodal
The aqueous solution of fluorescent carbon point the preparation method comprises the following steps: carbon source is taken to be dissolved in ethyl alcohol, obtain precursors；The reaction precursor that will be obtained
Body carries out 2~12h of hydro-thermal reaction at 120~240 DEG C, obtains unimodal fluorescent carbon point aqueous solution；
The aqueous solution of the bimodal fluorescent carbon point the preparation method comprises the following steps: carbon source is taken to be dissolved in ethyl alcohol, reproducibility nitrogen source is added, obtains
Precursors；Obtained precursors are subjected to 2~12h of hydro-thermal reaction at 120~240 DEG C, obtain multimodal fluorescent carbon
Point aqueous solution.
4. the method as claimed in claim 3 for identifying various metals ion by fluorescent carbon point, which is characterized in that the carbon source
Selected from 5- amino isophthalic acid, 2- amino isophthalic acid, 4- amino isophthalic acid, 2- amino terephthalic acid (TPA), 2,5-
Diamino terephthalic acid (TPA), ortho-aminobenzoic acid, gavaculine, p-aminobenzoic acid, 3- aminophthalic acid and 4-
One of aminophthalic acid.
5. the method as claimed in claim 3 for identifying various metals ion by fluorescent carbon point, which is characterized in that the reduction
Property nitrogen source be selected from ethylenediamine, ammonium hydroxide, ethanol amine, propylamine, 1,2- propane diamine, 1,3- propane diamine, hydrazine hydrate, triethylamine and diethylamine
One of.
6. the method as claimed in claim 3 for identifying various metals ion by fluorescent carbon point, which is characterized in that the carbon source
Molar ratio with reproducibility nitrogen source is 1:1~1:10.
7. the method as claimed in claim 3 for identifying various metals ion by fluorescent carbon point, which is characterized in that further include pair
The step of obtained fluorescent carbon point aqueous solution is post-processed；The post-processing are as follows: fluorescent carbon point aqueous solution is centrifuged to remove
Large granular impurity then carries out rotary evaporation, obtained powdered fluorescent carbon point deionized water dissolving.
8. the method as described in claim 1 for identifying various metals ion by fluorescent carbon point, which is characterized in that the fluorescence
The concentration of carbon dots aqueous solution is 0.001~0.01g/L, and the concentration of the aqueous metallic ions is 0.5~5mM.
9. the method as described in claim 1 for identifying various metals ion by fluorescent carbon point, which is characterized in that fluorescent carbon point
Aqueous solution and aqueous metallic ions volume ratio be 1:1~1:10.
10. a kind of detector for the method for implementing according to any one of claims 1 to 99 described in any item identification various metals ions.
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Cited By (3)
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| CN111024662A (en) * | 2019-12-11 | 2020-04-17 | 武汉大学 | Method for enhancing recognition capability of carbon dots on mercury ions |
| CN112742366A (en) * | 2019-10-29 | 2021-05-04 | 中国石油化工股份有限公司 | Nano carbon-based material and preparation method thereof and catalytic oxidation method of cycloparaffin |
| CN115353872A (en) * | 2022-07-14 | 2022-11-18 | 哈尔滨工业大学 | Chelate ligand functionalized carbon dots, high-yield synthesis method and application thereof |
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