CN113340884A - Buoy type online water quality detection device based on vibrio qinghai - Google Patents
Buoy type online water quality detection device based on vibrio qinghai Download PDFInfo
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Abstract
The invention relates to a buoy type online water quality detection device based on vibrio qinghai, which comprises a buoy shell, an indicator light strip, a bacteria liquid placing cavity, a selective permeation unit, an optical sensor, a data acquisition and control unit and a wireless signal receiving and transmitting unit, wherein the bacteria liquid placing cavity is arranged inside a lower hemisphere of the buoy shell, and a vibrio qinghai mixed liquid is arranged in the bacteria liquid placing cavity; the selective permeation unit is arranged on the lower hemispherical surface of the buoy shell, so that an external water sample enters the bacteria liquid placing cavity; the data acquisition and control unit acquires and stores the light-emitting information in real time and controls the light-emitting mode of the indicator light strip based on the light-emitting information. Compared with the prior art, the invention constructs a biosensor aiming at the conventional water environment monitoring based on the vibrio qinghai based on the biological toxicity detection principle of the vibrio qinghai of an environmental water sample, reflects the influence of water body pollutants on organisms and the water environment, and indirectly reflects the pollution degree of the water body.
Description
Technical Field
The invention relates to the field of water quality detection, in particular to a buoy type online water quality detection device based on vibrio qinghai.
Background
Bioluminescence is a very promising natural phenomenon, mainly accomplished by organisms such as algae and bacteria. Over the past few years, this life phenomenon has received much attention from scientists and designers, and has attempted to find innovative improvements therein to environmental issues. On the other hand, scientists have conducted quantitative analysis and evaluation of various pollutants in water body environment through various sophisticated instrumentation, however, such measurement means are often only capable of detecting a single target amount. In contrast, vibrio qinghaiensis can comprehensively react with the living water quality environment, and the novel life sensing material enables people to realize overall monitoring and comprehensive evaluation of environmental pollutants from the viewpoint of environmental protection.
Compared with other chemical or electronic instrument analysis technologies, the related technology of biotoxicity detection is still in the initial stage, has wide prospect and great potential, and still needs to be continuously developed and perfected. At present, the conventional biological toxicity detection of vibrio qinghaii is operated in a laboratory, and the detection process mainly comprises four steps: sampling on the spot, transporting the sample, mixed culturing and detecting by a microplate reader. This conventional detection approach has different problems at various stages: in-situ sampling requires a worker to perform in-situ multipoint sampling in a water area to be detected, so that manpower is wasted; the cost is increased due to long-distance transportation and storage in the sample transportation process, and meanwhile, the water sample stored for a long time is likely to deteriorate, so that the error of the experimental result is caused; the enzyme-linked immunosorbent assay is used for detection in a laboratory, and the price is high; a water sample with a limited volume cannot detect the water quality flowing in the natural environment; the complicated operation process of the microplate reader can only detect the luminous intensity of the vibrio qinghai at a certain time point, which is not beneficial to continuous observation.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide an online water quality detection device based on vibrio qinghaiensis.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides an aim at protects an online water quality testing device of buoy type based on vibrio qinghai, place chamber, the penetrating unit of selectivity, light sensor, data acquisition and the control unit and wireless signal receiving and dispatching unit including buoy shell, instruction lamp area, fungus liquid, wherein specifically:
the buoy shell is of a spherical structure;
the indicating lamp strip is arranged on the upper hemisphere surface of the buoy shell;
the bacteria liquid placing cavity is arranged inside the lower hemisphere of the buoy shell and is internally provided with a vibrio qinghai mixed liquid;
the selective permeation unit is arranged on the lower hemispherical surface of the buoy shell, so that an external water sample enters the bacteria liquid placing cavity;
the optical sensor is arranged above the bacteria liquid placing cavity and used for acquiring the light emitting information of the bacteria liquid placing cavity in real time;
the data acquisition and control unit is arranged in the buoy shell, is respectively electrically connected with the optical sensor and the indicator light strip, acquires and stores luminous information in real time, and controls the luminous mode of the indicator light strip based on the luminous information;
and the wireless signal receiving and transmitting unit is arranged in the buoy shell, is connected with the data acquisition and control unit and transmits the data acquired by the data acquisition and control unit to an external terminal.
Furthermore, the surface of the lower hemisphere of the buoy shell is provided with a sampling area with a specific size, the sampling area is of a net-shaped framework structure, and the selective transparent unit is arranged on the net-shaped framework structure.
Furthermore, the selective permeation unit is a selective permeation membrane, the selective permeation membrane is arranged on the net-shaped skeleton structure, so that water samples, pollutant molecules and nutrient molecules in the water samples selectively enter the bacteria liquid placing cavity, and meanwhile, vibrio qinghai is prevented from flowing outwards.
Further, the pore size of the selective permeation membrane is 0.22 μm.
Further, the fungus liquid placing cavity is a closed cavity, the upper portion of the fungus liquid placing cavity is sealed through a light-transmitting panel, a sampling hole is formed in the lower portion of the fungus liquid placing cavity, and the selective permeable membrane covers the outlet end of the sampling hole.
Further, the indicator light strip is a colored LED light strip.
Further, the lighting information includes lighting intensity and time point, the data acquisition and control unit compares the lighting intensity with a preset threshold value in real time, and calculates a difference between the lighting intensity and the preset threshold value, when the lighting intensity is greater than the threshold value, the data acquisition and control unit instructs the indicator light strip to display a specific color, and the displayed color depends on the size of the difference.
And the power supply unit is electrically connected with the indicator light band, the light sensor, the data acquisition and control unit and the wireless signal transceiving unit.
Further, the data acquisition and control unit is Arduino Nano V3.0 ATMEGA.
Further, the light sensor is an adahurit TSL2591 high dynamic range digital light sensor.
Compared with the prior art, the invention has the following technical advantages:
1) in the technical scheme, the Adafruit TSL2591 High Dynamic light sensor is adopted to monitor the luminous intensity of vibrio qinghaiensis in real time, the traditional ELISA reader detection method is replaced, the cost is lower, and the efficiency is higher.
2) The device compares the detecting instrument on the market and is small and exquisite light more, and built-in unlimited transmission module makes the user can monitor quality of water environment on the spot in various nature fresh water areas.
3) According to the technical scheme, intelligent detection can be realized, namely, a large amount of experimental data are screened according to the target amount, the data features are mapped to the pollution condition by using a machine learning algorithm, and a terminal user can directly obtain the information of the water pollution condition.
Drawings
FIG. 1 is a schematic structural diagram of a buoy-type online water quality detection device based on Vibrio qinghaiensis in the technical scheme;
fig. 2 is a schematic exploded part view of the buoy-type online water quality detection device based on vibrio qinghai in the technical scheme.
Fig. 3 is a schematic view of the operation principle of the buoy-type online water quality detection device based on vibrio qinghai in the technical scheme.
In the figure: 1. buoy housing, 2, instruction lamp area, 3, the solid fixed ring in lamp area, 4, electrical unit, 5, installation cavity, 6, fungus liquid place the chamber, 7, the penetrating unit of selectivity, 8, electrical unit, 9, data acquisition and the control unit, 10, wireless signal send-receiver unit, 11, optical sensor, 12, vibrio qinghai fungus liquid.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments.
The online quality detection device for the vibrio qinghai in the technical scheme is based on the biotoxicity detection principle of the vibrio qinghai in an environmental water sample, and a biosensor for routine water environment monitoring is constructed based on the vibrio qinghai, so that the influence of water pollutants on organisms and water environment is reflected, and the pollution degree of the water is indirectly reflected. The luminous intensity of the vibrio qinghaiensis is read in real time by culturing the vibrio qinghaiensis liquid on the spot, the luminous intensity is wirelessly transmitted to equipment terminals such as a computer, the receiving end calculates the luminous inhibition rate of water pollutants on the vibrio qinghaiensis through collected data, and the mapping between the received value and the environmental pollution is realized through program operation, which is shown in figure 3.
The purpose of this application is to solve a series of problems such as inevitable manpower, the wasting of resources, detection error, the operation is complicated, the instrument is expensive in the laboratory operation of current vibrio qinghai biological toxicity detection. The vibrio qinghaiensis online water quality detection device solves the series of problems in the aspects of detection principle and use scene. The water pollution degree is estimated by putting the detection device into a water area to be detected and detecting the change of the luminous intensity of the vibrio qinghaiensis on the spot.
The buoy-type online water quality detection device based on vibrio qinghai in the embodiment comprises a buoy shell 1, an indicator light strip 2, a bacteria liquid placing cavity 6, a selective permeation unit 7, an optical sensor 11, a data acquisition and control unit 9, a wireless signal transceiving unit 10 and a power supply unit 8, and is shown in fig. 1 and 2.
The power supply unit 8 is electrically connected with the indicator light band 2, the light sensor 11, the data acquisition and control unit 9 and the wireless signal receiving and transmitting unit 10, and a 6V lithium battery is selected during specific type selection.
Buoy housing 1 is spherical structure, and buoy housing 1 lower hemisphere surface is equipped with the sample area of specific size, the sample area is netted skeleton texture, the penetrating unit 7 of selectivity is located on the netted skeleton texture. The reticular skeleton structure is a prismatic hollow reticular structure, and can store a large amount of bubbles when entering water, so as to ensure that the oxygen content of the probe part is sufficient, and the growth and the propagation of the vibrio qinghai are facilitated. The buoy housing 1 is also provided with a mounting cavity 5 of a data acquisition and control unit 9. The bacteria liquid placing cavity 6 is arranged inside the lower hemisphere of the buoy shell 1, and vibrio qinghai bacteria liquid is arranged in the bacteria liquid placing cavity.
The indication lamp strip 2 is arranged on the upper hemisphere surface of the buoy shell 1, and the indication lamp strip 2 is a colorful LED lamp strip and can realize switching of multiple colors. The indicator light strip 2 is fixed on the buoy housing 1 through the lamp strip fixing ring 3.
The selective permeation unit 7 is arranged on the lower hemispherical surface of the buoy shell 1, so that an external water sample enters the bacteria liquid placing cavity 6. The selective permeable unit 7 is a selective permeable membrane which is arranged on the reticular skeleton structure, selectively enables the water sample, pollutant molecules and nutrient molecules in the water sample to enter the bacteria liquid placing cavity 6, and simultaneously prevents vibrio qinghai from flowing outwards. When the type is specifically selected, the selective permeable membrane is a high-molecular membrane material, and the aperture of the selective permeable membrane is 0.22 μm.
Data acquisition and the control unit 9 are located inside buoy housing 1, data acquisition and the control unit 9 respectively with light sensor 11 and indicator light area 2 electricity are connected, and data acquisition and the control unit 9 obtain and save the light emitting information in real time to based on the light emitting mode in light emitting information control indicator light area 2, during specific type selection, data acquisition and the control unit 9 are Arduino Nano V3.0 ATMEGA. The lighting information includes lighting intensity and time point, the data obtaining and controlling unit 9 compares the lighting intensity with a preset threshold value in real time, and calculates a difference between the lighting intensity and the preset threshold value, when the lighting intensity is greater than the threshold value, the data obtaining and controlling unit 9 instructs the indicator strip 2 to display a specific color, and the displayed color depends on the size of the difference. If the difference is within 30% of the threshold value, blue color is displayed, purple color is displayed when the difference is within 30% -70%, and red color is displayed when the difference is above 70%.
The wireless signal transceiver unit 10 is disposed inside the buoy housing 1, is connected to the data acquisition and control unit 9, and transmits data acquired by the data acquisition and control unit 9 to an external terminal for further data analysis and processing. When the model is selected specifically, the wireless signal transceiver unit 10 selects the HC-06 wireless bluetooth module.
The fungus liquid is placed chamber 6 and is closed cavity, the fungus liquid is placed the top of chamber 6 and is passed through the printing opacity panel and seal, the fungus liquid is placed the below of chamber 6 and has been seted up the thief hole, the selectively penetrating membrane cover in the exit end of thief hole.
The invention not only solves various problems possibly existing in the traditional laboratory detection mode, but also enables the detection method of the biological toxicity of the vibrio qinghaiensis to be possible from the laboratory to thousands of households by the detection device with low cost and small volume. The principle of Vibrio qinghaiensis biotoxicity detection is also suitable for water quality detection of domestic water, and in the future of the world of everything interconnection, the device can appear in the aspect of the scene of domestic water of people.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (10)
1. The utility model provides an online water quality testing device of buoy type based on vibrio qinghai which characterized in that includes:
the buoy shell (1) is of a spherical structure;
the indicating lamp strip (2) is arranged on the upper hemisphere surface of the buoy shell (1);
the bacteria liquid placing cavity (6) is arranged inside the lower hemisphere of the buoy shell (1), and is provided with vibrio qinghai bacteria liquid (12);
the selective permeation unit (7) is arranged on the lower hemispherical surface of the buoy shell (1) to enable an external water sample to enter the bacteria liquid placing cavity (6);
the optical sensor (11) is arranged above the bacteria liquid placing cavity (6) and is used for acquiring the light emitting information of the bacteria liquid placing cavity (6) in real time;
the data acquisition and control unit (9) is arranged inside the buoy shell (1), the data acquisition and control unit (9) is electrically connected with the optical sensor (11) and the indicator lamp strip (2) respectively, and the data acquisition and control unit (9) acquires and stores light-emitting information in real time and controls the light-emitting mode of the indicator lamp strip (2) based on the light-emitting information;
and the wireless signal receiving and transmitting unit (10) is arranged in the buoy shell (1), is connected with the data acquisition and control unit (9), and transmits the data acquired by the data acquisition and control unit (9) to an external terminal.
2. The buoy-type online water quality detection device based on vibrio qinghai of claim 1, wherein the surface of the lower hemisphere of the buoy housing (1) is provided with a sampling area with a specific size, the sampling area is of a net-shaped skeleton structure, and the selective permeation unit (7) is arranged on the net-shaped skeleton structure.
3. The buoy-type online water quality detection device based on vibrio qinghaiensis as claimed in claim 2, wherein the selective permeable unit (7) is a selective permeable membrane, the selective permeable membrane is arranged on the mesh skeleton structure, and selectively enables contaminant molecules and nutrient molecules in a water sample to enter the bacteria liquid placing cavity (6) and simultaneously prevents vibrio qinghaiensis from flowing out.
4. The buoy-type online water quality detection device based on vibrio qinghaiensis as claimed in claim 3, wherein the aperture of the selective permeable membrane is 0.22 μm.
5. The buoy type online water quality detection device based on vibrio qinghaiensis as claimed in claim 3, wherein the bacteria liquid placing cavity (6) is a closed cavity, the upper part of the bacteria liquid placing cavity (6) is sealed by a light-transmitting panel, a sampling hole is arranged below the bacteria liquid placing cavity (6), and the selective permeable membrane covers the outlet end of the sampling hole.
6. The buoy-type online water quality detection device based on vibrio qinghaiensis as claimed in claim 1, wherein the indicator strip (2) is a colored LED strip.
7. The buoyage type online water quality detection device based on vibrio qinghaiensis as claimed in claim 6, wherein the light emitting information comprises light emitting intensity and time point, the data acquisition and control unit (9) compares the light emitting intensity with a preset threshold value in real time, and calculates the difference between the light emitting intensity and the preset threshold value, when the light emitting intensity is greater than the threshold value, the data acquisition and control unit (9) instructs the indicator strip (2) to display a specific color, and the displayed color depends on the magnitude of the difference.
8. The buoy-type online water quality detection device based on vibrio qinghaiensis as claimed in claim 1, further comprising a power supply unit (8), wherein the power supply unit (8) is electrically connected with the indicator strip (2), the optical sensor (11), the data acquisition and control unit (9) and the wireless signal transceiving unit (10).
9. The buoy-type online water quality detection device based on vibrio qinghai of claim 1, characterized in that the data acquisition and control unit (9) is Arduino Nano V3.0 ATMEGA.
10. The buoy-type online water quality detection device based on vibrio qinghaiensis as claimed in claim 1, wherein the optical sensor (11) is an adaflur TSL2591 high dynamic range digital optical sensor.
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| CN117382811A (en) * | 2023-10-19 | 2024-01-12 | 河北省张家口水文勘测研究中心 | Novel hydrologic test buoy device and system |
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| US7690247B1 (en) * | 2007-03-20 | 2010-04-06 | The United States Of America As Represented By The Secretary Of The Navy | Autonomous biobuoy for detecting a characteristic of a marine biosphere and method of assembling the biobuoy |
| CN101915759A (en) * | 2010-07-20 | 2010-12-15 | 同济大学 | Vibrio qinghaiensis Q67 based long-term microplate toxicity analyzing method of environmental pollutant |
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