CN113330992A

CN113330992A - Incubator for strain cultivation and growth and cultivation method thereof

Info

Publication number: CN113330992A
Application number: CN202110770575.1A
Authority: CN
Inventors: 程安东; 汪本勤
Original assignee: Luan Vocational and Technical College
Current assignee: Luan Vocational and Technical College
Priority date: 2021-07-08
Filing date: 2021-07-08
Publication date: 2021-09-03
Anticipated expiration: 2041-07-08
Also published as: CN113330992B

Abstract

The invention discloses an incubator for strain cultivation and growth and a cultivation method thereof, and relates to the technical field of strain cultivation. The invention comprises a box body, a bottom plate and a support frame, wherein the box body is at least provided with three side plates, one side plate is provided with a channel and a jack, and the other side plate is provided with a blind hole corresponding to the jack; the bottom plate is provided with a concave surface, and the extractable point of the bottom plate is matched on the channel of the box body; one end of the supporting frame is matched with the blind hole after penetrating through the jack, the concave surface faces the supporting frame when the bottom plate is installed on the channel, a water inlet hole is formed in the side plate, and a water dropper is installed on the water inlet hole. According to the invention, the detachable box body bottom plate is designed and replaced every day, so that the influence of bacteria breeding in a container for storing water on the initial normal growth of phellinus igniarius is avoided.

Description

Incubator for strain cultivation and growth and cultivation method thereof

Technical Field

The invention belongs to the technical field of strain cultivation, and particularly relates to an incubator for strain cultivation and growth and a cultivation method thereof.

Background

Phellinus linteus is a medicinal fungus internationally recognized for treating cancer, and has the effects of regulating immunity, resisting oxidation and the like. In recent years, the demand of phellinus igniarius in the industries of pharmaceutical preparations and health care products is increasing, and phellinus igniarius cultivation mainly comprises two types of bag cultivation and cut-log cultivation. The bag material culture is to put a culture medium into a plastic bag, inoculate after sterilization, then place the plastic bag in a proper environment for culture until fruiting bodies grow out, the other type of cut wood culture is to place cut wood sections into a fungus bag, lay the culture medium on the sections at two ends, then tie up the fungus bag, inoculate after sterilization, carry out fruiting culture after the fungus bag is full of hypha, the colony control in the incubator is not emphasized in the fruiting culture initial stage of the fungus bag at present, namely the fungus bags are more than one box body in the culture initial stage to the middle stage, the bottom of the box body will nourish a large amount of bacteria in the greenhouse environment, and the normal growth of phellinus igniarius which just cut the bags is influenced.

Disclosure of Invention

The invention aims to provide an incubator for strain cultivation and growth and a cultivation method thereof.

In order to solve the technical problems, the invention is realized by the following technical scheme:

the invention relates to a strain cultivation and growth incubator, which comprises a case body, a bottom plate and a support frame, wherein the case body is at least provided with three side plates, one side plate is provided with a channel and an insertion hole, and the other side plate is provided with a blind hole corresponding to the insertion hole;

the bottom plate is provided with a concave surface, and the extractable point of the bottom plate is matched on the channel of the box body;

one end of the supporting frame is matched on the blind hole after penetrating through the jack, and the concave surface faces the supporting frame when the bottom plate is installed on the channel.

Furthermore, a water inlet hole is formed in the side plate, and a water dropper is installed on the water inlet hole.

Furthermore, the side plate is at least provided with two mounting holes, a water release device is mounted in one mounting hole, the bottom plate is a first integral piece formed by the pulling plate and the plate body, the concave surface is arranged on the surface of the plate body, and the plate body is provided with a notch communicated with the water release device and the concave surface.

Further, place the board on the support frame, place the board and include orifice plate or porous plastic board, the lower surface of orifice plate and porous plastic board all has the cardboard.

Further, a case lid has on the box, has the detection regulating part on the case lid, and the detection regulating part includes temperature and humidity inductor and sets up the circle that ventilates on temperature and humidity inductor, and the circle that ventilates is including deciding board and movable plate, decides to have the spout on the board, and the movable plate is along the removal of spout.

Furthermore, through holes are formed in the box cover and the side plates, and ventilators are installed on the through holes.

Further, the ventilator includes an outer sleeve and an inner tube, each of the outer sleeve and the inner tube having a pair of semicircular plates, one end of the inner tube having a notch for engaging the semicircular plates, and when a semicircular plate in the outer sleeve is fully engaged in the notch, a cross-section of the semicircular plate in the inner tube and a semicircular plate in the outer sleeve is intended to close the inner tube.

A method for culturing a strain in an incubator, which comprises the following steps:

regulating and controlling the temperature: placing the box body indoors, and controlling the indoor temperature to be 25-28 ℃;

regulating and controlling humidity: sterilizing and disinfecting the inside of the incubator, storing water on the concave surface of the plate body, putting the plate body into the incubator, covering the incubator cover, controlling the on-off number of the ventilators according to the indoor environment temperature and the air flow speed, and adjusting the humidity in the incubator body;

culturing phellinus igniarius: a support frame is inserted into the box body, and a phellinus igniarius fungus bag is placed on the support frame.

Furthermore, a water dropper is used for pouring water into the concave surface on the plate body, the humidity in the box body is kept, the lamp is turned off in the morning and at the evening every day, and the ventilation ring is turned on for ventilation for 5-15 min.

Furthermore, the sterilized clean bottom plate is replaced before ventilation in the morning and at the evening every day, wherein the concave surface on the plate body is filled with clean water.

The invention has the following beneficial effects:

the detachable bottom plate of the box body is designed and replaced every day, so that the situation that bacteria breeding in a container for storing water influences the initial normal growth of phellinus igniarius is avoided, the water outlet device sucks water exceeding a set height on the concave surface through the notch, the existing water quantity in the box body is ensured, the opening number of the ventilators is controlled in a matching mode, the air circulation speed in the box body is determined, and the humidity and the temperature in the box body are regulated and controlled.

Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic view of the structure of an incubator;

FIG. 2 is a schematic structural diagram of a case;

FIG. 3 is a schematic view of the case cover;

FIG. 4 is a schematic structural view of a base plate;

FIG. 5 is a schematic view of the construction of the ventilator;

FIG. 6 shows a box body with a porous plastic plate;

FIG. 7 shows a perforated plate and a perforated plastic plate box;

FIG. 8 is a schematic diagram of the construction of an orifice plate;

FIG. 9 is a schematic view of the structure of the vent collar;

in the drawings, the components represented by the respective reference numerals are listed below:

1. a box body; 2. a base plate; 3. a support frame; 4. a water discharge device; 5. a ventilator; 6. a box cover; 7. a through hole; 8. a detection adjustment unit; 9. placing the plate; 101. a side plate; 102. blind holes; 103. a channel; 104. a jack; 201. pulling a plate; 202. a sealing groove; 203. a plate body; 204. a concave surface; 205. a notch; 501. an inner tube; 502. a semicircular plate; 503. a notch; 504. an outer sleeve; 801. a temperature and humidity sensor; 802. a ventilation ring; 803. fixing a plate; 804. a movable plate; 901. an orifice plate; 902. a porous plastic plate; 903. and (4) clamping the board.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "opening," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like are used in an orientation or positional relationship that is merely for convenience in describing and simplifying the description, and do not indicate or imply that the referenced component or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present invention.

Referring to fig. 1-9, the present invention is an incubator for cultivating and growing Phellinus linteus in a traditional culture mode, which mainly comprises a case 1, a bottom plate 2, a support frame 3 and a case cover 6.

The cabinet 1 has at least three side panels 101. One of the side plates 101 has a channel 103 and a receptacle 104, and the other side plate 101 has a blind hole 102 corresponding to the receptacle 104.

The preferred box 1 is a rectangular box with four sides, the bearing capacity is larger compared with three-side triangular holding type, the inserting holes 104 and the blind holes 102 are respectively arranged on the front plate and the rear plate of the box 1, and the support 3 can be used for erecting phellinus igniarius fungus bags after being arranged on the inserting holes 104 and the blind holes 102.

Specifically, one end of the support frame 3 penetrates through the jack 104 and then is matched with the blind hole 102, the support frame 3 is a three-inserting-rod type support frame, namely the jack 104 and the blind hole 102 are respectively in a group of three holes, three inserting rods on one support frame 3 are linked by one connecting rod, and the support frame 3 is integrally pulled and pulled through the pulling of the connecting rod.

The bottom plate 2 is provided with a concave surface 204, the drawable point of the bottom plate 2 is matched on the channel 103 of the box body 1, wherein the concave surface 204 forms a shallow water surface, namely, the total amount of water is small, the evaporation speed of the water is easier to be regulated and controlled by temperature, namely, the humidity in the box body is more convenient to control, specifically, the bottom plate 2 is a first integral part formed by the pulling plate 201 and the plate body 203, and the concave surface 204 is arranged on the surface of the plate body 203.

Wherein the contact edge of the plate body 203 and the pulling plate 201 is provided with a bee groove 202 for filling the sealing rubber strip.

In addition, the concave surface 204 faces the support frame 3 when the base plate 2 is mounted on the channel 103.

When the bottom plate 2 is installed, the concave surface is firstly filled outside the box body 1 and then the box body is placed in the box body.

The side plate 101 has a water inlet hole, the water dropper 10 is installed on the water inlet hole, and the total water amount on the bottom plate 2 is kept stable by the dropping speed of the water dropper 10.

The side plates 101 are at least provided with two mounting holes, the specific mounting holes are arranged on the side plates 101 on two sides of the box body 1, a water drainage device 4 is arranged in one mounting hole, water exceeding a set height on the concave surface 204 is sucked out through the water drainage device 4, a notch 205 communicated with the water drainage device 4 and the concave surface 204 is formed in the plate body 203, the water drainage device 4 is a sponge water absorption device, and an inclined plane inclined towards the mounting hole is formed in the notch 205.

The placing plate 9 is placed on the supporting frame 3, the placing plate 9 includes a hole plate 901 or a porous plastic plate 902, as shown in fig. 7 and 8, the porous plastic plate 902 can be placed on the supporting frame in a row, and if the space allowance between two rows of the porous plastic plates 902 is large, the hole plate 901 can be placed on the supporting frame.

The lower surfaces of the pore plate 901 and the porous plastic plate 902 are provided with clamping plates 903, and the pore plate 901 and the porous plastic plate 902 are fixed on the support frame 3 through the clamping plates 903.

In addition, a box cover 6 is arranged on the box body 1, a detection adjusting part 8 is arranged on the box cover 6, and the detection adjusting part 8 comprises a temperature and humidity sensor 801 and a ventilation ring 802 arranged on the temperature and humidity sensor 801.

Specifically, the detection adjusting part 8 is provided with a temperature sensor and a humidity sensor, so as to obtain the temperature and humidity parameters of the box body 1, and adjust and control the box body 1 according to the temperature and humidity parameters.

The ventilating ring 802 comprises a fixed plate 803 and a movable plate 804, the fixed plate 803 is provided with a sliding groove, the movable plate 804 moves along the sliding groove, namely the opening size of the ventilating ring 802 is controlled by dragging the movable plate 804, and the ventilator 5 is combined to adjust the wind speed flow in the box body 1.

Specifically, the method comprises the following steps: both the box cover 6 and the side plate 101 are provided with through holes 7, and the ventilator 5 is arranged on the through holes 7.

Further, the ventilator 5 comprises an outer sleeve 504 and an inner tube 501, the outer sleeve 504 and the inner tube 501 are both provided with a pair of semicircular plates 502, one end of the inner tube 501 is provided with a notch 503 matched with the semicircular plates 502, when the semicircular plates 502 in the outer sleeve 504 are completely matched in the notch 503, the cross sections of the semicircular plates 502 in the inner tube 501 and the semicircular plates 502 in the outer sleeve 504 are supposed to abut against and close the inner tube 501, the ventilator 5 is closed after the inner tube 501 is pressed down, and specifically, the ventilation speed in the box body 1 can be determined by controlling the opening number of the ventilator 5, so that the humidity and the temperature in the box body 1 are influenced.

A method for culturing Phellinus linteus by using an incubator for strain culture and growth comprises the following steps:

regulating and controlling the temperature: the box body 1 is arranged indoors, the indoor temperature is controlled to be 25-28 ℃, and the temperature of the working environment of the box body 1 is preferably controlled.

Regulating and controlling humidity: the incubator is internally sterilized and disinfected, the concave surface 204 on the plate body 203 stores water, the incubator 1 is placed in, the incubator cover 6 is covered, the on-off number of the ventilator 5 is controlled according to the indoor environment temperature and the air flow speed, and the humidity in the incubator 1 is adjusted.

Culturing phellinus igniarius: a supporting frame 3 is inserted into the box body 1, phellinus igniarius fungus bags are placed on the supporting frame 3, and the space between the fungus bags is 100 mm.

Further, the water dropper 10 is used for pouring water into the concave surface 204 on the plate body 203, the humidity in the box body 1 is kept, the lamp is turned off in the morning and at the evening every day, the ventilation ring 802 is turned on for ventilation for 5-15min, and indoor air supply flows above the box body 1.

Further, the sterilized clean bottom plate 2 is replaced before ventilation in the morning and evening every day, wherein the concave surface 204 on the plate body 203 is filled with clean water.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. The utility model provides a fungus is cultivated growth and is used incubator which characterized in that includes:

the box body (1), the box body (1) has three side plates (101) at least, there are channel (103) and jack (104) on one side plate (101), there are blind holes (102) corresponding to the jack (104) on another side plate (101);

the bottom plate (2), the concave surface (204) is arranged on the bottom plate (2), and the extractable point of the bottom plate (2) is matched on the channel (103) of the box body (1);

one end of the support frame (3) penetrates through the jack (104) and then is matched with the blind hole (102), and the concave surface (204) faces the support frame (3) when the bottom plate (2) is installed on the channel.

2. The incubator as claimed in claim 1, wherein the side plate (101) has a water inlet hole, and the water dropper (10) is installed on the water inlet hole.

3. An incubator as claimed in claim 2, wherein the side plate (101) has at least two holes, a drain (4) is mounted in one hole, the bottom plate (2) is a first integral member formed by the pulling plate (201) and the plate body (203), the concave surface (204) is disposed on the surface of the plate body (203), and the plate body (203) has a slot (205) communicating the drain (4) and the concave surface (204).

4. The incubator according to claim 3, wherein the supporting frame (3) is provided with a placing plate (9), the placing plate (9) comprises a hole plate (901) or a porous plastic plate (902), and the lower surfaces of the hole plate (901) and the porous plastic plate (902) are provided with clamping plates (903).

5. The incubator according to claim 3, wherein the incubator body (1) has a cover (6), the cover (6) has a detecting and adjusting portion (8), the detecting and adjusting portion (8) comprises a temperature and humidity sensor (801) and a ventilation ring (802) disposed on the temperature and humidity sensor (801), the ventilation ring (802) comprises a fixed plate (803) and a movable plate (804), the fixed plate (803) has a sliding slot, and the movable plate (804) moves along the sliding slot.

6. An incubator as claimed in any one of claims 1 to 5, wherein the cover (6) and the side plate (101) each have a through hole (7), the through holes (7) being provided with a ventilator (5).

7. An incubator as claimed in claim 6, wherein the ventilator (5) comprises an outer tube (504) and an inner tube (501), the outer tube (504) and the inner tube (501) each having a pair of semicircular plates (502), the inner tube (501) having a notch (503) at one end for engaging the semicircular plates (502), the inner tube (501) having a semicircular plate (502) therein and a semicircular plate (502) in the outer tube (504) having a cross-section for abutting against the closed inner tube (501) when the semicircular plate (502) in the outer tube (504) is fully engaged in the notch (503).

8. A method of culturing a culture box for strain growth using the culture box for strain growth according to claim 7, comprising the steps of:

regulating and controlling the temperature: placing the box body (1) indoors, and controlling the indoor temperature to be 25-28 ℃;

regulating and controlling humidity: sterilizing and disinfecting the inside of the incubator (1), storing water in the concave surface (204) on the plate body (203), putting the incubator (1) into the incubator, covering the incubator cover (6), controlling the on-off number of the ventilators (5) according to the indoor environment temperature and the air flow speed, and adjusting the humidity in the incubator body (1);

culturing phellinus igniarius: a supporting frame (3) is inserted in the box body (1), and a phellinus igniarius fungus bag is placed on the supporting frame (3).

9. The method of claim 8, wherein the water is dripped into the concave surface (204) of the plate (203) by using a water dropper (10), the humidity in the box (1) is maintained, the light is turned off at the early and late times every day, and the ventilation ring (802) is turned on to ventilate for 5-15 min.

10. The method of claim 9, wherein the sterilized clean bottom plate (2) is replaced before the ventilation is performed in the morning and evening of the day, and wherein the concave surface (204) of the plate body (203) is filled with clear water.