CN113321727B - 一种识别afp抗原短肽的t细胞受体及其编码序列 - Google Patents

一种识别afp抗原短肽的t细胞受体及其编码序列 Download PDF

Info

Publication number
CN113321727B
CN113321727B CN202010130645.2A CN202010130645A CN113321727B CN 113321727 B CN113321727 B CN 113321727B CN 202010130645 A CN202010130645 A CN 202010130645A CN 113321727 B CN113321727 B CN 113321727B
Authority
CN
China
Prior art keywords
tcr
seq
amino acid
ser
chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010130645.2A
Other languages
English (en)
Chinese (zh)
Other versions
CN113321727A (zh
Inventor
李懿
张龙兴
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xiangxue Life Science Technology Guangdong Co ltd
Original Assignee
Xiangxue Life Science Technology Guangdong Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xiangxue Life Science Technology Guangdong Co ltd filed Critical Xiangxue Life Science Technology Guangdong Co ltd
Priority to CN202010130645.2A priority Critical patent/CN113321727B/zh
Priority to TW110107140A priority patent/TW202140539A/zh
Priority to PCT/CN2021/078277 priority patent/WO2021170117A1/fr
Publication of CN113321727A publication Critical patent/CN113321727A/zh
Application granted granted Critical
Publication of CN113321727B publication Critical patent/CN113321727B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503Immunoglobulin superfamily
    • C07K14/7051T-cell receptor (TcR)-CD3 complex
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/177Receptors; Cell surface antigens; Cell surface determinants
    • A61K38/1774Immunoglobulin superfamily (e.g. CD2, CD4, CD8, ICAM molecules, B7 molecules, Fc-receptors, MHC-molecules)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/461Cellular immunotherapy characterised by the cell type used
    • A61K39/4611T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/463Cellular immunotherapy characterised by recombinant expression
    • A61K39/4632T-cell receptors [TCR]; antibody T-cell receptor constructs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/464Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643Vertebrate antigens
    • A61K39/4644Cancer antigens
    • A61K39/46448Cancer antigens from embryonic or fetal origin
    • A61K39/464481Alpha-feto protein
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • C12N15/867Retroviral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0636T lymphocytes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/15011Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
CN202010130645.2A 2020-02-28 2020-02-28 一种识别afp抗原短肽的t细胞受体及其编码序列 Active CN113321727B (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202010130645.2A CN113321727B (zh) 2020-02-28 2020-02-28 一种识别afp抗原短肽的t细胞受体及其编码序列
TW110107140A TW202140539A (zh) 2020-02-28 2021-02-26 一種辨識afp抗原短鏈胜肽的t細胞受體及其編碼序列
PCT/CN2021/078277 WO2021170117A1 (fr) 2020-02-28 2021-02-26 Récepteur de lymphocytes t reconnaissant un peptide court d'antigène afp et séquence de codage de celui-ci

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010130645.2A CN113321727B (zh) 2020-02-28 2020-02-28 一种识别afp抗原短肽的t细胞受体及其编码序列

Publications (2)

Publication Number Publication Date
CN113321727A CN113321727A (zh) 2021-08-31
CN113321727B true CN113321727B (zh) 2024-04-09

Family

ID=77412833

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010130645.2A Active CN113321727B (zh) 2020-02-28 2020-02-28 一种识别afp抗原短肽的t细胞受体及其编码序列

Country Status (3)

Country Link
CN (1) CN113321727B (fr)
TW (1) TW202140539A (fr)
WO (1) WO2021170117A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112898399A (zh) * 2019-12-03 2021-06-04 香雪生命科学技术(广东)有限公司 源自于afp抗原的短肽
CN113321727B (zh) * 2020-02-28 2024-04-09 香雪生命科学技术(广东)有限公司 一种识别afp抗原短肽的t细胞受体及其编码序列

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019036688A1 (fr) * 2017-08-18 2019-02-21 Gritstone Oncology, Inc. Protéines de liaison d'antigène ciblant des antigènes partagés
CN110343166A (zh) * 2018-04-03 2019-10-18 广东香雪精准医疗技术有限公司 识别afp抗原短肽的t细胞受体
CN110577591A (zh) * 2018-06-08 2019-12-17 广东香雪精准医疗技术有限公司 一种识别afp抗原短肽的t细胞受体及其编码序列
WO2021170117A1 (fr) * 2020-02-28 2021-09-02 香雪生命科学技术(广东)有限公司 Récepteur de lymphocytes t reconnaissant un peptide court d'antigène afp et séquence de codage de celui-ci
CN113667008A (zh) * 2020-05-15 2021-11-19 香雪生命科学技术(广东)有限公司 一种识别afp抗原的高亲和力t细胞受体

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201313377D0 (en) * 2013-07-26 2013-09-11 Adaptimmune Ltd T cell receptors
CN104087592B (zh) * 2014-05-13 2016-01-27 天津医科大学总医院 Afp158-166特异性tcr基因及其转基因t细胞及体外增殖方法及用途
CN110776562B (zh) * 2018-07-30 2022-06-17 香雪生命科学技术(广东)有限公司 一种识别afp抗原的t细胞受体

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019036688A1 (fr) * 2017-08-18 2019-02-21 Gritstone Oncology, Inc. Protéines de liaison d'antigène ciblant des antigènes partagés
CN110343166A (zh) * 2018-04-03 2019-10-18 广东香雪精准医疗技术有限公司 识别afp抗原短肽的t细胞受体
CN110577591A (zh) * 2018-06-08 2019-12-17 广东香雪精准医疗技术有限公司 一种识别afp抗原短肽的t细胞受体及其编码序列
WO2021170117A1 (fr) * 2020-02-28 2021-09-02 香雪生命科学技术(广东)有限公司 Récepteur de lymphocytes t reconnaissant un peptide court d'antigène afp et séquence de codage de celui-ci
CN113667008A (zh) * 2020-05-15 2021-11-19 香雪生命科学技术(广东)有限公司 一种识别afp抗原的高亲和力t细胞受体

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
"T细胞克隆与甲胎蛋白相关的研究进展";王晓雷等;医学理论与实践;第25卷(第2期);152-154 *
Identification of α-fetoprotein-specific T-cell receptors for hepatocellular carcinoma immunotherapy;Wei Zhu等;Hepatology;第68卷(第2期);574-589 *

Also Published As

Publication number Publication date
CN113321727A (zh) 2021-08-31
TW202140539A (zh) 2021-11-01
WO2021170117A1 (fr) 2021-09-02

Similar Documents

Publication Publication Date Title
CN112646024B (zh) 一种识别kras突变的t细胞受体及其编码序列
WO2020063488A1 (fr) Récepteur de lymphocytes t reconnaissant un antigène ssx2
CN106749620B (zh) 识别mage-a1抗原短肽的t细胞受体
CN106831978B (zh) 识别prame抗原的t细胞受体
CN110343166B (zh) 识别afp抗原短肽的t细胞受体
CN110776562B (zh) 一种识别afp抗原的t细胞受体
CN113321726B (zh) 一种识别hpv的t细胞受体
CN110577591B (zh) 一种识别afp抗原短肽的t细胞受体及其编码序列
CN109575121B (zh) 识别afp抗原短肽的t细胞受体
CN113072635B (zh) 一种识别hpv抗原的t细胞受体及其编码序列
WO2021022447A1 (fr) Récepteur de slymphocytes t capable de reconnaître un peptide court dérivé d'un antigène afp
WO2021016887A1 (fr) Récepteur de lymphocytes t reconnaissant le peptide court de l'antigène ssx2
CN113321727B (zh) 一种识别afp抗原短肽的t细胞受体及其编码序列
CN113321725B (zh) 一种识别afp的t细胞受体
CN109400697B (zh) 一种识别prame抗原短肽的tcr及其相关组合物
CN108948184B (zh) 一种识别衍生自prame抗原短肽的t细胞受体
CN113072636B (zh) 一种识别afp的t细胞受体及其编码序列
CN110577590B (zh) 一种识别afp抗原的tcr及其编码核酸
CN110407927B (zh) 一种识别afp抗原的tcr
CN110627894B (zh) 识别ny-eso-1抗原短肽的t细胞受体及其编码序列
CN109400696B (zh) 一种识别prame抗原短肽的tcr
CN109575120B (zh) 识别prame抗原短肽的tcr及其编码序列
CN110272483B (zh) 识别sage1抗原短肽的t细胞受体
WO2022206861A1 (fr) Récepteur de lymphocytes t pour l'identification d'afp
WO2022262835A1 (fr) Tcr pour identifier un antigène afp et séquence codant pour celui-ci

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant