CN113308498A - Method for preparing bioethanol from pineapple leaves - Google Patents
Method for preparing bioethanol from pineapple leaves Download PDFInfo
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- CN113308498A CN113308498A CN202110577763.2A CN202110577763A CN113308498A CN 113308498 A CN113308498 A CN 113308498A CN 202110577763 A CN202110577763 A CN 202110577763A CN 113308498 A CN113308498 A CN 113308498A
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- pineapple leaves
- bioethanol
- raw material
- juice
- pineapple
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- 235000007119 Ananas comosus Nutrition 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 25
- 244000099147 Ananas comosus Species 0.000 title 1
- 241000234671 Ananas Species 0.000 claims abstract description 73
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 28
- 239000002994 raw material Substances 0.000 claims abstract description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 19
- 230000000694 effects Effects 0.000 claims abstract description 17
- 239000011259 mixed solution Substances 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000005520 cutting process Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 abstract description 10
- 230000007613 environmental effect Effects 0.000 abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000000446 fuel Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for preparing bioethanol by using pineapple leaves as a raw material, which comprises the following steps: firstly, collecting pineapple leaves and pretreating the pineapple leaves; step two, squeezing the pretreated pineapple leaves to separate juice and sterilizing the juice; adding activated high-activity yeast into the juice, and fermenting to obtain a mixed solution containing bioethanol; and step four, extracting the bioethanol in the mixed solution, wherein the preparation method provided by the invention creatively adopts the pineapple leaves with high content of reducing sugar as the raw material, and converts the reducing sugar in the pineapple leaves into the bioethanol by taking fermentation as a main method, so that the treatment on the pineapple leaves is scientific and effective, the use cost of the raw material for preparing the bioethanol is greatly reduced, and the preparation method has extremely high environmental and economic values.
Description
Technical Field
The invention relates to the technical field of bioethanol preparation, in particular to a method for preparing bioethanol by taking pineapple leaves as a raw material.
Background
The bioethanol is prepared by converting various biomasses into fuel alcohol through microbial fermentation, and can be used as automobile fuel by being prepared into ethanol gasoline alone or together with gasoline, currently, in the preparation of bioethanol in China, aged grains such as corn and wheat are mainly used as raw materials, and starch or sugar is converted into ethanol through biological fermentation, wherein the corn accounts for 78% of the total raw materials, the corn ethanol in China is subjected to large-scale production, and the adopted raw materials are mainly grains such as corn, so that the production cost of the bioethanol is high, and the preparation of the bioethanol has great limitation.
Pineapple is used as a famous tropical crop, is popular with people due to good taste and low price, is planted in a large scale in the global scope, is used as a pineapple planting kingdom in China, is mainly produced in places such as Guangdong, Hainan, Guangxi, Yunnan, Fujian and Taiwan province, the yield of the pineapple is increased year by year, and in 2018, the annual yield of the pineapple in China reaches 162.5 ten thousand tons, the planting area reaches 7 ten thousand hectares, a large amount of waste pineapple leaves are generated after pineapple fruits are harvested, and are randomly stacked, discarded or burned in concentration, so that the resource is greatly wasted while the environment is polluted, the juice yield of the pineapple leaves is as high as 70%, the juice contains rich reducing sugar, and can be used as a preparation raw material of bioethanol, and how to prepare bioethanol through the pineapple leaves is a problem which needs to be solved at present.
Disclosure of Invention
In view of the above, the present invention is directed to a method for preparing bioethanol from pineapple leaves, which solves all or one of the above problems of the prior art.
Based on the above purpose, the invention provides a method for preparing bioethanol by using pineapple leaves as a raw material, which comprises the following steps:
firstly, collecting pineapple leaves and pretreating the pineapple leaves;
step two, squeezing the pretreated pineapple leaves to separate juice and sterilizing the juice;
adding activated high-activity yeast into the juice, and fermenting to obtain a mixed solution containing bioethanol;
and step four, extracting the bioethanol in the mixed solution.
Optionally, the step one specifically includes the following steps:
collecting and cutting pineapple leaves, and discarding the pineapple leaves which are mildewed or seriously damaged;
washing the pineapple leaves to remove dirt on the surfaces of the pineapple leaves;
the pineapple leaves are dried and cut into small pieces.
Optionally, the length of the small blocks in the step of cutting the pineapple leaves into small blocks after air drying is 3-5 cm.
Optionally, the sterilization conditions in the second step are as follows: keeping the temperature at 110-120 ℃ and the pressure at 0.08-0.12 MPa in a closed container for 15-25 min.
Optionally, the fermentation conditions in the third step are as follows: fermenting at 25-35 deg.C for 18-24 hr, and stirring the mixed solution of juice and high activity yeast at 100 rpm.
Optionally, the volume ratio of the high-activity yeast to the juice in the third step is 1: 100.
Optionally, the step four specifically includes the following steps:
centrifuging the mixed solution, and taking supernatant;
distilling the supernatant to obtain the bioethanol.
Optionally, the activation method of the high-activity yeast in step three is as follows: mixing high activity dried yeast powder and sterile water at a volume ratio of 1:10, maintaining the temperature at 35-38 deg.C, and activating in water bath for 15-20 min.
From the above, the method for preparing bioethanol from pineapple leaves provided by the invention creatively adopts the pineapple leaves with high content of reducing sugar as the raw material, and converts reducing sugar in the pineapple leaves into bioethanol by taking fermentation as a main method, so that the pineapple leaves are scientifically and effectively treated, the use cost of the raw materials for preparing bioethanol is greatly reduced, and the method has extremely high environmental and economic values.
Drawings
In order to more clearly illustrate one or more embodiments or prior art solutions of the present specification, the drawings that are needed in the description of the embodiments or prior art will be briefly described below, and it is obvious that the drawings in the following description are only one or more embodiments of the present specification, and that other drawings may be obtained by those skilled in the art without inventive effort from these drawings.
FIG. 1 is a schematic diagram of a preparation process of the present invention;
FIG. 2 is the experimental data of bioethanol preparation from multiple sets of pineapple leaf samples according to the present invention.
Detailed Description
To make the objects, technical solutions and advantages of the present disclosure more apparent, the present disclosure is further described in detail below with reference to specific embodiments.
It is to be noted that unless otherwise defined, technical or scientific terms used in one or more embodiments of the present specification should have the ordinary meaning as understood by those of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in one or more embodiments of the specification is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
One or more embodiments of the present specification disclose a method for preparing bioethanol from pineapple leaves, comprising the steps of:
firstly, collecting pineapple leaves and pretreating the pineapple leaves;
step two, squeezing the pretreated pineapple leaves to separate juice and sterilizing the juice;
adding activated high-activity yeast into the juice, and fermenting to obtain a mixed solution containing bioethanol;
and step four, extracting the bioethanol in the mixed solution.
As shown in figure 1, the invention provides a method for preparing bioethanol from pineapple leaves, which comprises the steps of collecting pineapple leaves after picking fruits, pretreating the pineapple leaves, squeezing the pretreated pineapple leaves to obtain a large amount of juice, using the residual residue as a fuel, separating the juice, sterilizing to avoid yeast pollution in the fermentation process, adding high-activity yeast into the sterilized juice, converting reducing sugar in the juice into bioethanol through fermentation, mixing the bioethanol in a container after fermentation, and extracting the bioethanol in the mixed solution.
The preparation method provided by the invention creatively adopts the pineapple leaves with high reducing sugar content as the raw material, and converts the reducing sugar in the pineapple leaves into the bioethanol by taking fermentation as a main method, so that the pineapple leaves are scientifically and effectively treated, the use cost of the raw material for preparing the bioethanol is greatly reduced, and the preparation method has extremely high environmental and economic values.
In some optional embodiments, the step one specifically includes the following steps:
collecting and cutting pineapple leaves, and discarding the pineapple leaves which are mildewed or seriously damaged;
washing the pineapple leaves to remove dirt on the surfaces of the pineapple leaves;
the pineapple leaves are dried and cut into small pieces.
The pineapple leaves which are mildewed and the like are removed, and meanwhile, the pineapple leaves are cleaned, so that impurities can be prevented from being mixed into juice, yeast is prevented from being polluted, and the pineapple leaves are cut into small pieces which are convenient to squeeze fully.
In some optional embodiments, the length of the small pieces in the step of cutting the pineapple leaves into small pieces after air drying is 3-5 cm.
In some optional embodiments, the sterilization conditions in step two are: keeping the temperature at 110-120 ℃ and the pressure at 0.08-0.12 MPa in a closed container for 15-25 min.
In some alternative embodiments, the fermentation conditions in step three are: fermenting at 25-35 deg.C for 18-24 hr, and stirring the mixed solution of juice and high activity yeast at 100 rpm.
In some alternative embodiments, the volume ratio of the high activity yeast to the juice in step three is 1: 100.
In some optional embodiments, the step four specifically includes the following steps:
centrifuging the mixed solution, and taking supernatant;
distilling the supernatant to obtain the bioethanol.
The fermented mixed solution contains bioethanol and other impurities, after centrifugal treatment, the impurities are settled to the bottom, the bioethanol is located in the supernatant, the supernatant is taken out, and the bioethanol is separated out in a distillation mode to obtain a bioethanol solution with high purity.
In some alternative embodiments, the activation method of the high-activity yeast in step three is as follows: mixing high activity dried yeast powder and sterile water at a volume ratio of 1:10, maintaining the temperature at 35-38 deg.C, and activating in water bath for 15-20 min.
As shown in fig. 2, the experimental data show that the content of reducing sugar in pineapple leaf juice is about 42g/L, and the theoretical bioethanol yield is 2.71% (V/V) based on the content of reducing sugar, that is, one volume of juice can yield 2.71% of bioethanol, but the experiment shows that the actual bioethanol yield reaches 3.2% (V/V), and the juice contains other carbon sources to participate in bioethanol production, so that the bioethanol yield of pineapple leaves is far greater than the theoretical value, and is very suitable for being used as a raw material to prepare bioethanol.
The foregoing description has been directed to specific embodiments of this disclosure. Other embodiments are within the scope of the following claims. In some cases, the actions or steps recited in the claims may be performed in a different order than in the embodiments and still achieve desirable results. In addition, the processes depicted in the accompanying figures do not necessarily require the particular order shown, or sequential order, to achieve desirable results. In some embodiments, multitasking and parallel processing may also be possible or may be advantageous.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the spirit of the present disclosure, features from the above embodiments or from different embodiments may also be combined, steps may be implemented in any order, and there are many other variations of different aspects of one or more embodiments of the present description as described above, which are not provided in detail for the sake of brevity.
In addition, well-known power/ground connections to Integrated Circuit (IC) chips and other components may or may not be shown in the provided figures, for simplicity of illustration and discussion, and so as not to obscure one or more embodiments of the disclosure. Furthermore, devices may be shown in block diagram form in order to avoid obscuring the understanding of one or more embodiments of the present description, and this also takes into account the fact that specifics with respect to implementation of such block diagram devices are highly dependent upon the platform within which the one or more embodiments of the present description are to be implemented (i.e., specifics should be well within purview of one skilled in the art). Where specific details (e.g., circuits) are set forth in order to describe example embodiments of the disclosure, it should be apparent to one skilled in the art that one or more embodiments of the disclosure can be practiced without, or with variation of, these specific details. Accordingly, the description is to be regarded as illustrative instead of restrictive.
While the present disclosure has been described in conjunction with specific embodiments thereof, many alternatives, modifications, and variations of these embodiments will be apparent to those of ordinary skill in the art in light of the foregoing description. For example, other memory architectures (e.g., dynamic ram (dram)) may use the discussed embodiments.
It is intended that the one or more embodiments of the present specification embrace all such alternatives, modifications and variations as fall within the broad scope of the appended claims. Therefore, any omissions, modifications, substitutions, improvements, and the like that may be made without departing from the spirit and principles of one or more embodiments of the present disclosure are intended to be included within the scope of the present disclosure.
Claims (8)
1. A method for preparing bioethanol from pineapple leaves is characterized by comprising the following steps:
firstly, collecting pineapple leaves and pretreating the pineapple leaves;
step two, squeezing the pretreated pineapple leaves to separate juice and sterilizing the juice;
adding activated high-activity yeast into the juice, and fermenting to obtain a mixed solution containing bioethanol;
and step four, extracting the bioethanol in the mixed solution.
2. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 1, wherein said first step specifically comprises the following steps:
collecting and cutting pineapple leaves, and discarding the pineapple leaves which are mildewed or seriously damaged;
washing the pineapple leaves to remove dirt on the surfaces of the pineapple leaves;
the pineapple leaves are dried and cut into small pieces.
3. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 2, wherein the length of the small pieces in the step of cutting the pineapple leaves into small pieces after air-drying the pineapple leaves is 3-5 cm.
4. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 1, wherein the sterilization conditions in the second step are as follows: keeping the temperature at 110-120 ℃ and the pressure at 0.08-0.12 MPa in a closed container for 15-25 min.
5. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 1, wherein the fermentation conditions in the third step are as follows: fermenting at 25-35 deg.C for 18-24 hr, and stirring the mixed solution of juice and high activity yeast at 100 rpm.
6. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 1, wherein the volume ratio of the high-activity yeast to the juice in step three is 1: 100.
7. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 1, wherein said step four specifically comprises the steps of:
centrifuging the mixed solution, and taking supernatant;
distilling the supernatant to obtain the bioethanol.
8. The method for preparing bioethanol from pineapple leaves as a raw material according to claim 1, wherein the activation method of the high-activity yeast in step three comprises: mixing high activity dried yeast powder and sterile water at a volume ratio of 1:10, maintaining the temperature at 35-38 deg.C, and activating in water bath for 15-20 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110577763.2A CN113308498A (en) | 2021-05-26 | 2021-05-26 | Method for preparing bioethanol from pineapple leaves |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110577763.2A CN113308498A (en) | 2021-05-26 | 2021-05-26 | Method for preparing bioethanol from pineapple leaves |
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| CN113308498A true CN113308498A (en) | 2021-08-27 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201114904A (en) * | 2009-10-30 | 2011-05-01 | Atomic Energy Council | A method for enhancing the ethanol concentration from the conversion of lignocelluloses |
| WO2018231052A1 (en) * | 2017-06-14 | 2018-12-20 | Innovative Sustainable Technologies B.V. | Process for obtaining cellulose from plant material |
| CN109153987A (en) * | 2016-06-21 | 2019-01-04 | 国立研究开发法人国际农林水产业研究中心 | Microorganism, for the preparation method of the composition of decomposing lignocellulose biolobic material, the preparation method of saccharified liquid and lignocellulose-like biomass derivative compound |
-
2021
- 2021-05-26 CN CN202110577763.2A patent/CN113308498A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201114904A (en) * | 2009-10-30 | 2011-05-01 | Atomic Energy Council | A method for enhancing the ethanol concentration from the conversion of lignocelluloses |
| CN109153987A (en) * | 2016-06-21 | 2019-01-04 | 国立研究开发法人国际农林水产业研究中心 | Microorganism, for the preparation method of the composition of decomposing lignocellulose biolobic material, the preparation method of saccharified liquid and lignocellulose-like biomass derivative compound |
| WO2018231052A1 (en) * | 2017-06-14 | 2018-12-20 | Innovative Sustainable Technologies B.V. | Process for obtaining cellulose from plant material |
Non-Patent Citations (5)
| Title |
|---|
| ANJANI DEVI CHINTAGUNTA 等: ""An integrated bioprocess for bioethanol and biomanure production from pineapple leaf waste"", 《JOURNAL OF CLEANER PRODUCTION》 * |
| 叶明: "《微生物学实验技术》", 31 August 2016, 合肥工业大学出版社 * |
| 周常勇: "《中国果树科学与实践 柑橘》", 31 August 2020, 陕西科学技术出版社 * |
| 李亚丽 等: ""食品废弃物资源化利用研究进展"", 《现代化工》 * |
| 黄国成 等: "《菠萝栽培技术问答》", 30 September 1999, 中国盲文出版社 * |
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