CN113304086A - 一种多维屏障修复组合物及其制备方法 - Google Patents
一种多维屏障修复组合物及其制备方法 Download PDFInfo
- Publication number
- CN113304086A CN113304086A CN202110574579.2A CN202110574579A CN113304086A CN 113304086 A CN113304086 A CN 113304086A CN 202110574579 A CN202110574579 A CN 202110574579A CN 113304086 A CN113304086 A CN 113304086A
- Authority
- CN
- China
- Prior art keywords
- component
- skin
- barrier
- extract
- repair composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000004888 barrier function Effects 0.000 title claims abstract description 105
- 239000000203 mixture Substances 0.000 title claims abstract description 73
- 230000008439 repair process Effects 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 210000003491 skin Anatomy 0.000 claims abstract description 54
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 20
- 150000002632 lipids Chemical class 0.000 claims abstract description 16
- 239000002904 solvent Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000008591 skin barrier function Effects 0.000 claims abstract description 13
- 238000005728 strengthening Methods 0.000 claims abstract description 12
- 102000000591 Tight Junction Proteins Human genes 0.000 claims abstract description 11
- 108010002321 Tight Junction Proteins Proteins 0.000 claims abstract description 11
- 239000008367 deionised water Substances 0.000 claims abstract description 11
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 11
- 239000003960 organic solvent Substances 0.000 claims abstract description 11
- 201000004624 Dermatitis Diseases 0.000 claims abstract description 10
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 9
- 239000004615 ingredient Substances 0.000 claims abstract description 9
- 230000003204 osmotic effect Effects 0.000 claims abstract description 9
- 230000001737 promoting effect Effects 0.000 claims abstract description 9
- 230000001105 regulatory effect Effects 0.000 claims abstract description 9
- 102000003728 Peroxisome Proliferator-Activated Receptors Human genes 0.000 claims abstract description 7
- 108090000029 Peroxisome Proliferator-Activated Receptors Proteins 0.000 claims abstract description 7
- 230000003213 activating effect Effects 0.000 claims abstract description 7
- 210000002615 epidermis Anatomy 0.000 claims abstract description 6
- 229940067606 lecithin Drugs 0.000 claims abstract description 5
- 239000000787 lecithin Substances 0.000 claims abstract description 5
- 235000010445 lecithin Nutrition 0.000 claims abstract description 5
- 239000000284 extract Substances 0.000 claims description 35
- 235000019198 oils Nutrition 0.000 claims description 15
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 14
- 235000012000 cholesterol Nutrition 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 239000006166 lysate Substances 0.000 claims description 6
- 235000020238 sunflower seed Nutrition 0.000 claims description 6
- ATGQXSBKTQANOH-UWVGARPKSA-N N-oleoylphytosphingosine Chemical compound CCCCCCCCCCCCCC[C@@H](O)[C@@H](O)[C@H](CO)NC(=O)CCCCCCC\C=C/CCCCCCCC ATGQXSBKTQANOH-UWVGARPKSA-N 0.000 claims description 5
- 229940044176 ceramide 3 Drugs 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 3
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 claims description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 3
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 3
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 3
- 229920002498 Beta-glucan Polymers 0.000 claims description 3
- SNCIAAAFBLUWEE-NMTVEPIMSA-N CSCC[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(C)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](C)C(O)=O Chemical compound CSCC[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(C)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](C)C(O)=O SNCIAAAFBLUWEE-NMTVEPIMSA-N 0.000 claims description 3
- 240000004385 Centaurea cyanus Species 0.000 claims description 3
- 235000005940 Centaurea cyanus Nutrition 0.000 claims description 3
- 241000218378 Magnolia Species 0.000 claims description 3
- 244000183331 Nephelium lappaceum Species 0.000 claims description 3
- 239000005642 Oleic acid Substances 0.000 claims description 3
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 3
- 241000727169 Prinsepia utilis Species 0.000 claims description 3
- 229940093797 bioflavonoids Drugs 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 3
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 3
- 235000021313 oleic acid Nutrition 0.000 claims description 3
- 235000007861 rambutan Nutrition 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 3
- 238000003892 spreading Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 108010082495 Dietary Plant Proteins Proteins 0.000 claims description 2
- 244000248557 Ophiopogon japonicus Species 0.000 claims description 2
- 244000047670 Viola x wittrockiana Species 0.000 claims description 2
- 235000004031 Viola x wittrockiana Nutrition 0.000 claims description 2
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 2
- 230000014759 maintenance of location Effects 0.000 claims description 2
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 claims description 2
- 239000012466 permeate Substances 0.000 claims description 2
- 241001478778 Cladophora Species 0.000 claims 1
- 230000035515 penetration Effects 0.000 abstract description 14
- 238000004090 dissolution Methods 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 description 29
- 230000000638 stimulation Effects 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 230000001154 acute effect Effects 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 239000002537 cosmetic Substances 0.000 description 6
- 230000007794 irritation Effects 0.000 description 6
- 230000004044 response Effects 0.000 description 6
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 206010067739 Shrinking lung syndrome Diseases 0.000 description 5
- 206010048676 Sjogren-Larsson Syndrome Diseases 0.000 description 5
- 206010040880 Skin irritation Diseases 0.000 description 5
- 238000010166 immunofluorescence Methods 0.000 description 5
- 210000002510 keratinocyte Anatomy 0.000 description 5
- 231100000286 mucous membrane, eye irritation or corrosion testing Toxicity 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000036556 skin irritation Effects 0.000 description 5
- 231100000475 skin irritation Toxicity 0.000 description 5
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 102000002029 Claudin Human genes 0.000 description 3
- 108050009302 Claudin Proteins 0.000 description 3
- 102100023593 Fibroblast growth factor receptor 1 Human genes 0.000 description 3
- 101000827746 Homo sapiens Fibroblast growth factor receptor 1 Proteins 0.000 description 3
- 101000917159 Homo sapiens Filaggrin Proteins 0.000 description 3
- 102100021948 Lysyl oxidase homolog 2 Human genes 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 108020000318 saccharopine dehydrogenase Proteins 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108010058734 transglutaminase 1 Proteins 0.000 description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 2
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 2
- 241000700198 Cavia Species 0.000 description 2
- 102100040836 Claudin-1 Human genes 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 102100027723 Endogenous retrovirus group K member 6 Rec protein Human genes 0.000 description 2
- 101710091045 Envelope protein Proteins 0.000 description 2
- 101000749331 Homo sapiens Claudin-1 Proteins 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 102100020881 Interleukin-1 alpha Human genes 0.000 description 2
- 108010082786 Interleukin-1alpha Proteins 0.000 description 2
- 108090001007 Interleukin-8 Proteins 0.000 description 2
- 102000004890 Interleukin-8 Human genes 0.000 description 2
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 2
- 101710188315 Protein X Proteins 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000011449 brick Substances 0.000 description 2
- 229940106189 ceramide Drugs 0.000 description 2
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000006003 cornification Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 210000000744 eyelid Anatomy 0.000 description 2
- 229960001340 histamine Drugs 0.000 description 2
- 229920002674 hyaluronan Polymers 0.000 description 2
- 229960003160 hyaluronic acid Drugs 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 2
- 230000037311 normal skin Effects 0.000 description 2
- 230000000474 nursing effect Effects 0.000 description 2
- 238000005191 phase separation Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 210000002374 sebum Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 1
- OAVCWZUKQIEFGG-UHFFFAOYSA-O 2-(5-methyl-2H-tetrazol-1-ium-1-yl)-1,3-thiazole Chemical compound CC1=NN=N[NH+]1C1=NC=CS1 OAVCWZUKQIEFGG-UHFFFAOYSA-O 0.000 description 1
- 102100032282 26S proteasome non-ATPase regulatory subunit 14 Human genes 0.000 description 1
- 241000049624 Alisma plantago-aquatica subsp. orientale Species 0.000 description 1
- 102000010637 Aquaporins Human genes 0.000 description 1
- 108010063290 Aquaporins Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102100032912 CD44 antigen Human genes 0.000 description 1
- 102000004958 Caspase-14 Human genes 0.000 description 1
- 108090001132 Caspase-14 Proteins 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 102000010970 Connexin Human genes 0.000 description 1
- 108050001175 Connexin Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- 102000002045 Endothelin Human genes 0.000 description 1
- 108050009340 Endothelin Proteins 0.000 description 1
- 101000590281 Homo sapiens 26S proteasome non-ATPase regulatory subunit 14 Proteins 0.000 description 1
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 description 1
- 101001054921 Homo sapiens Lymphatic vessel endothelial hyaluronic acid receptor 1 Proteins 0.000 description 1
- 101001114059 Homo sapiens Protein-arginine deiminase type-1 Proteins 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 102100026849 Lymphatic vessel endothelial hyaluronic acid receptor 1 Human genes 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 102100038831 Peroxisome proliferator-activated receptor alpha Human genes 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 102100038280 Prostaglandin G/H synthase 2 Human genes 0.000 description 1
- 108050003267 Prostaglandin G/H synthase 2 Proteins 0.000 description 1
- 102100030944 Protein-glutamine gamma-glutamyltransferase K Human genes 0.000 description 1
- 102000013968 STAT6 Transcription Factor Human genes 0.000 description 1
- 108010011005 STAT6 Transcription Factor Proteins 0.000 description 1
- 239000010404 Scutellaria baicalensis extract Substances 0.000 description 1
- 206010040844 Skin exfoliation Diseases 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- -1 ZO-1 Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000005482 chemotactic factor Substances 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000035618 desquamation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- ZUBDGKVDJUIMQQ-UBFCDGJISA-N endothelin-1 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@H]2CSSC[C@@H](C(N[C@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@@H](CO)NC(=O)[C@H](N)CSSC1)C1=CNC=N1 ZUBDGKVDJUIMQQ-UBFCDGJISA-N 0.000 description 1
- 230000003090 exacerbative effect Effects 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036074 healthy skin Effects 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000006371 metabolic abnormality Effects 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 108091008725 peroxisome proliferator-activated receptors alpha Proteins 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008263 repair mechanism Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/16—Amides, e.g. hydroxamic acids
- A61K31/164—Amides, e.g. hydroxamic acids of a carboxylic acid with an aminoalcohol, e.g. ceramides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
- A61K31/201—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having one or two double bonds, e.g. oleic, linoleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/575—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/04—Rhodophycota or rhodophyta (red algae), e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/55—Linaceae (Flax family), e.g. Linum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
- A61K36/575—Magnolia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/86—Violaceae (Violet family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/884—Alismataceae (Water-plantain family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8968—Ophiopogon (Lilyturf)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9717—Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Birds (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Dermatology (AREA)
- Organic Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Rheumatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Neurosurgery (AREA)
- Pain & Pain Management (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
Abstract
本发明公开了一种多维屏障修复组合物，包括功效复合物、渗透平衡剂和溶剂；所述功效复合物包括：功效成分A，能够促进角化包膜和紧密连接蛋白表达的成分；功效成分B，能够提升皮肤自主保湿力的成分；功效成分C，能够激活PPAR受体、强化皮肤脂质屏障的成分；功效成分D，能够调节免疫屏障并减轻皮肤炎症的成分；功效成分E，能够平衡表皮微生态的成分；渗透平衡剂包括卵磷脂和/或氢化卵磷脂中的一种或多种，维持所有功效成分安全有效地渗透至皮肤屏障的不同位点，是使组合物发挥功效的重要成分；溶剂包括去离子水和有机溶剂。本发明还公开了一种多维屏障修复组合物的制备方法，其能够实现功效复合物、渗透平衡剂和溶剂在组合物中的有效共溶。
Description
技术领域
本发明涉及一种多维屏障修复组合物及其制备方法，属于医药和化妆品领域。
背景技术
皮肤是人体最大的器官，皮肤屏障就构成了人体和外界最大的一道保护层，既可以减少皮下水分和活性物质的流失，又能够有效缓解外界紫外线、粉尘颗粒等因素带来的刺激。
完整的皮肤屏障通常由三大板块由外而内组成：微生物屏障、物理屏障以及免疫屏障。微生物屏障主要由皮肤表面的益生菌群构成，通过正常的微生物活动形成良好的皮肤微生态；物理屏障即通常所说的角质层，角质层包含了神经酰胺、胆甾醇、游离脂肪酸和卵磷脂等形成紧密的皮脂膜，各层细胞ZO-1、CLDN-1、Caspase14等紧密连接蛋白进行粘连，其间填充透明质酸等天然保湿因子，使皮肤充盈饱满，形成稳定的“砖墙结构”，对外界刺激形成物理阻隔；免疫屏障维持着皮肤对外界刺激的正常响应，当前两道屏障受损，刺激深入免疫屏障导致过度免疫，产生组胺以及IL-1α、IL-8、TNF-α、PGE-2等炎性细胞因子，最终导致皮肤红肿痒痛，进一步影响皮肤正常代谢功能；
皮肤屏障受损的因素主要包括以下途径：1.包含空气污染、紫外线、温湿度等因素在内的外界刺激；2.人为对皮肤不得当的保养，如过度清洁、使用不适合的化妆品等；3.内源性因素，包括情绪、压力等。以上因素共同造成皮肤屏障受损，导致表皮微生态失衡，角质层“砖墙”结构松散，有害微生物侵袭，角质层失水，炎性细胞浸润，皮肤代谢异常，不良情绪和压力增大，这些结果会反过来使皮肤屏障进一步瓦解，形成恶性循环。
由于皮肤屏障受损往往集干燥脱屑、瘙痒炎症、皮脂过剩等多种敏感症状于一体，且引发机制具有多样性，可调控的反应机制也因此较为复杂，市场上虽已有针对屏障修复的护理方案，但往往只包含部分机制，并不全面。更重要的是，屏障受损皮肤和正常皮肤的渗透性具有较大差异，即使适用于正常皮肤的活性物浓度，可能反而会对屏障受损皮肤造成刺激，从而加重受损程度。据相关报道，全球皮肤屏障受损的人群接近56％，且该数字仍在逐年上涨。因此，开发一种经屏障受损皮肤试验、具有适宜渗透性、功效相对全面的屏障修复组合物已成为当下需要解决的问题。
目前市场上屏障修复相关产品主要针对以下几个途径：1：“三明治”结构修复，通过外部涂抹神经酰胺、胆甾醇对皮肤缺失脂质进行填充；2：通过多糖等保湿剂缓解皮肤干燥失水；3：少数报道通过促进细胞分泌相关因子，从而提升皮肤自身屏障能力；虽然以上几个角度可以一定程度上缓解屏障受损程度，但其实皮肤对外源性脂质和保湿剂吸收相对有限，同时，由于细胞实验的测试浓度与化妆品中添加浓度相距甚远，若无相关渗透试验加以佐证，单一从细胞层面修复屏障的可参考性就会较低。
目前未见有报道从安全有效的渗透性角度出发，包含功效成分和渗透平衡剂，且能够多维全面修复皮肤屏障的组合物。
发明内容
本发明的目的是解决现有技术中存在的问题，提供一种多维屏障修复组合物，从促进角化包膜和紧密连接蛋白表达、提升皮肤自主保湿力、强化皮肤脂质、调节免疫屏障并减轻皮肤炎症和平衡表皮微生态五个维度出发，对受损屏障全方位有针对性的进行修复，在此基础上增加了渗透平衡剂，并应用一定的制备方法，使该组合物在屏障受损的皮肤上具有适宜的渗透力，既不会对屏障受损的皮肤造成进一步的刺激，又能够全面有效地修复屏障，可以应用于水剂、面膜、膏霜乳液中，能够进行安全有效的渗透，从而达到较好的屏障修复效果。
本发明的另外一个目的是提供一种多维屏障修复组合物的制备方法，能够实现功效复合物、渗透平衡剂和溶剂在组合物中的有效共溶，以及在屏障受损皮肤上安全有效地渗透，从而共同作用实现上述功效，而且保证制剂安全温和可靠，同时该组合物稳定性良好，在室温条件下保存时，不会发生分相，且易于应用在化妆品或医药外用制剂中。
实现上述目的一种技术方案是：一种多维屏障修复组合物，包括以下重量份的组份：
功效复合物 20～50份；
渗透平衡剂 2～20份；
溶剂 30～60份；
所述功效复合物包括：
功效成分A，能够促进角化包膜和紧密连接蛋白表达的成分；
功效成分B，能够提升皮肤自主保湿力的成分；
功效成分C，能够激活PPAR受体、强化皮肤脂质屏障的成分；
功效成分D，能够调节免疫屏障并减轻皮肤炎症的成分；
功效成分E，能够平衡表皮微生态的成分；
所述渗透平衡剂包括卵磷脂和/或氢化卵磷脂，所述渗透平衡剂维持所有功效成分安全有效地渗透至皮肤屏障的不同位点，使多维屏障修复组合物发挥功效；
所述溶剂包括去离子水和有机溶剂。
上述的一种多维屏障修复组合物，其中，所述功效成分A、功效成分B、功效成分C、功效成分D、功效成分E各占功效复合物总重量的10～30％
上述的一种多维屏障修复组合物，其中，所述能够促进角化包膜和紧密连接蛋白表达的成分包括具距石枝藻提取物、红毛丹果皮提取物、麦冬提取物中的一种或多种；
所述能够提升皮肤自主保湿力的成分包括生物类黄酮、水解植物蛋白、长角豆籽提取物、水解三色堇提取物、低聚果糖中的一种或多种；
所述能够激活PPAR受体、强化皮肤脂质屏障的成分包括神经酰胺3、胆甾醇和油脂；
所述能够调节免疫屏障并减轻皮肤炎症的成分包括欧泽泻提取物、柑橘皮提取物、β-葡聚糖、玉兰花提取物、牡丹皮提取物、黄芩提取物、矢车菊花提取物中的一种或多种；
所述能够平衡表皮微生态的成分包括乳酸菌发酵溶胞产物、乙酰基七肽-4、酵母提取物、亚麻籽提取物、乳酸杆菌发酵溶胞产物中的一种或多种。
上述的一种多维屏障修复组合物，其中，所述能够激活PPAR受体、强化皮肤脂质屏障的成分中，所述神经酰胺3、胆甾醇和油脂的重量比是9∶4∶0.5～9∶4∶2。
上述的一种多维屏障修复组合物，其中，所述油脂为油酸、亚油酸、青刺果油、向日葵籽油、向日葵籽油不皂化物中的一种或多种。
上述的一种多维屏障修复组合物，其中，所述有机溶剂为1,2-戊二醇、丁二醇和甘油中的一种或多种。
上述的一种多维屏障修复组合物，其中，所述去离子水的重量占所述溶剂的总重量的5～20％。
本发明还提供了上述的一种多维屏障修复组合物的制备方法，其特征在于，包括以下步骤：
S1，在称量好的有机溶剂中加入功效成分C，加热至70～75℃，搅拌至完全溶解，随后降温至40～45℃得到预配相1；
S2，将功效成分A、功效成分B、功效成分D、功效成分E以及去离子水常温混合并搅拌均匀，得到预配相2；
S3，将渗透平衡剂均匀铺展于固定平面，使其形成薄膜，缓慢注入预配相1和预配相2，旋转震荡使其水合，即得到所述多维屏障修复组合物。
采用本发明的多维屏障修复组合物的技术方案，从促进角化包膜和紧密连接蛋白表达、提升皮肤自主保湿力、强化皮肤脂质、调节免疫屏障并减轻皮肤炎症和平衡表皮微生态五个维度出发，对受损屏障全方位有针对性的进行修复，在此基础上增加了渗透平衡剂，使该组合物在屏障受损的皮肤上具有适宜的渗透力，既不会对屏障受损的皮肤造成进一步的刺激，又能够全面有效地修复屏障，同时该组合物在室温条件下保存时稳定性保持良好。
采用本发明的多维屏障修复组合物的制备方法的技术方案，其能够实现功效复合物、渗透平衡剂和溶剂在组合物中的有效共溶，以及在屏障受损皮肤上进行安全有效的渗透，从而共同作用达到坚固角质细胞、强化脂质屏障、提升自主保湿力、调节免疫屏障并减轻皮肤炎症、平衡皮肤微生态的功效，同时使得该组合物在室温条件下保存时稳定性保持良好。
附图说明
图1为免疫荧光法测定紧密连接蛋白含量实验中ZO-1蛋白的积分光密度图；
图2为免疫荧光法测定紧密连接蛋白含量实验中CLDN1蛋白的积分光密度图。
图3为EpiKuits表皮模型测定角化包膜试验中FLG蛋白的积分光密度图。
图4为EpiKuits表皮模型测定角化包膜试验中LOR蛋白的积分光密度图。
图5为EpiKuits表皮模型测定个角化包膜试验中TGM1蛋白的积分光密度图。
具体实施方式
为了使本技术领域的技术人员能更好地理解本发明的技术方案，下面结合附图对其具体实施方式进行详细地说明：
表1
按照表1的配方制备实施例1～5的多维屏障修复组合物，具体的制备工艺为：
S1，将称量好的有机溶剂中加入功效成分C，加热至70～75℃，搅拌至完全溶解，随后降温至40～45℃得到预配相1；
S2，将功效成分A、功效成分B、功效成分D、功效成分E以及去离子水常温混合并搅拌均匀，得到预配相2；
S3，将渗透平衡剂均匀铺展于固定平面，使其形成薄膜，缓慢注入预配相1和预配相2，旋转震荡使其水合，即得到所述多维屏障修复组合物。
本发明的多维屏障修复组合物，针对皮肤屏障修复机理，从五个维度出发，对受损屏障全方位有针对性的进行修复，其中，具距石枝藻提取物、红毛丹果皮提取物、麦冬提取物能够激活CLDN-1、PAD1、ZO-1、内皮蛋白、兜甲蛋白等紧密连接和角化包膜蛋白，同时促进角化包膜相关基因的表达，从而达到坚固角质细胞的作用，为功效成分A；生物类黄酮、水解植物蛋白、长角豆籽提取物、水解三色堇提取物、低聚果糖能够激活AQP 3水通道蛋白赫和VDR信号传导，增加透明质酸受体CD44合成，促进内源性透明质酸生成，调节Ca2+流量，平衡细胞渗透压，从而提升皮肤自主保湿能力，为功效成分B；神经酰胺3、胆甾醇、油酸、亚油酸、青刺果油、向日葵籽油、向日葵籽油不皂化物能够形成仿生皮脂膜，并激活PPAR-α受体，改善皮肤自身代谢能力，从而强化皮肤脂质屏障，为功效成分C；欧泽泻提取物、柑橘皮提取物、玉兰花提取物、牡丹皮提取物、β-葡聚糖、黄芩提取物、矢车菊花提取物能够减少组胺和IL-1α、IL-8、TNF-α、PGE-2等炎性细胞因子表达，抑制JAK/STAT6通路，降低COX-2活性，中和趋化因子，调节免疫屏障，从而起到修复抗炎的作用,为功效成分D；乳酸菌发酵溶胞产物、乙酰基七肽-4、酵母提取物、亚麻籽提取物、乳酸杆菌发酵溶胞产物能够给皮肤提供有益微生物喜欢的益生元和益生素，促进有益微生物群的生长，抵御有害微生物的侵袭，从而平衡表皮生态环境,为功效成分E。
本发明特别重视功效成分在屏障受损皮肤上的渗透功效和温和性，相比具有完整屏障功效的健康皮肤，屏障受损的皮肤对外源物刺激的响应会更加强烈，其中包括功效成分带来的有利影响，因此本发明在针对不同机理全方位设计功效复合物的基础上，设计了渗透平衡剂，渗透平衡剂包括卵磷脂和/或氢化卵磷脂，渗透平衡剂维持所有功效成分安全有效地渗透至皮肤屏障的不同位点，使多维屏障修复组合物发挥功效。
本发明的多维屏障修复组合物中，溶剂包括去离子水和有机溶剂，在多维屏障修复组合物的制备方法中，去离子水和有机溶剂的作用不同，有机溶剂与功效成分C溶解后得到预配相1；将功效成分A、功效成分B、功效成分D、功效成分E以及去离子水常温混合并搅拌均匀后得到预配相2；将渗透平衡剂均匀铺展于固定平面，使其形成薄膜，缓慢注入预配相1和预配相2，旋转震荡使其水合，即得到多维屏障修复组合物，这种制备方法，使多维屏障修复组合物形成部分包裹、整体具有浓度梯度的剂型，且用于包裹的原料(即渗透平衡剂)自身具有修护皮脂和保湿的功效，最终达到事半功倍的屏障修复效果。
本发明的多维屏障修复组合物及其制备方法，其能够实现功效复合物、渗透平衡剂和溶剂在组合物中的有效共溶，以及在屏障受损皮肤上进行安全有效的渗透，从而共同作用达到坚固角质细胞、强化脂质屏障、提升自主保湿力、调节免疫屏障并减轻皮肤炎症、平衡皮肤微生态的功效，同时该组合物在室温条件下保存时稳定性保持良好。
稳定性考察测试：
对实施例1～5的多维屏障修复组合物的进行稳定性考察测试，依次在25℃、40℃、49℃、-15℃和5℃条件下，分别测试30天，60天及90天，观察一种多维屏障修复组合物的分相情况，测试结果如表2所示：
表2
表2中，实施例1～5的多维屏障修复组合物在25℃、40℃、49℃、-15℃、5℃的条件下，90天均未发生分相，说明本发明的多维屏障修复组合物稳定性良好。
安全性评价：
多次皮肤刺激试验：
对实施例1～5的多维屏障修复组合物进行多次皮肤刺激试验，每个实施例均选择4只白色豚鼠进行实验，试验室温度：20-24℃，相对湿度：60-70％。试验前约24h，将实验动物背部脊柱两侧被毛剪掉，去毛范围各为2cm×3cm，涂抹面积2.5cm×2.5cm。取受试物约0.5mL涂抹在一侧皮肤上，当受试物使用无刺激性溶剂配制时，另一侧涂溶剂作为对照，每天涂抹1次，连续涂抹14天。从第二天开始，每次涂抹前应剪毛，用水或无刺激性溶剂清除残留受试物，一小时后观察结果。参照《化妆品安全技术规范(2015版)》规定进行刺激反应评分。急性皮肤刺激试验结果如表3所示：
表3
由表3急性皮肤刺激试验结果可知，实施例1～5的多维屏障修复组合物对豚鼠的急性皮肤刺激试验，均未见刺激性反应。因此本发明的多维屏障修复组合物，具有良好的安全性。
急性眼刺激试验：
选取实施例4的多维屏障修复组合物进行急性眼刺激试验，选择3只健康成年白色家兔进行试验，试验室温度：20-24℃，相对湿度：60-70％。试验前动物要在实验动物房环境中至少适应3天时间。轻轻拉开家兔一侧眼睛的下眼睑，将受试物0.1mL(100mg)滴入(或涂入)结膜囊中，使上、下眼睑被动闭合1s，以防止受试物丢失。另一侧眼睛不处理作自身对照。滴入受试物后24小时内不冲洗眼睛。若认为必要，在24h时可进行冲洗。若上述试验结果显示受试物有刺激性，需另选用3只家兔进行冲洗效果试验，即给家兔眼滴入受试物后30s用足量、流速较快但又不会引起动物眼损伤的水流冲洗至少30s。在滴入受试物后1、24、48、72小时以及第4滴和第7滴对动物眼睛进行检查。如果72小时未出现刺激反应，即可终止试验。如果发现累及角膜或有其它眼刺激作用，7天内不恢复者，为确定该损害的可逆性或不可逆性需延长观察时间，一般不超过21天。参照《化妆品安全技术规范(2015版)》规定进行刺激反应评分。急性眼刺激试验结果见表4：
表4
由表4的急性眼刺激实验结果可知，实施例4的多维屏障修复组合物对家兔的眼刺激性试验未见刺激性反应。因此本发明的多维屏障修复组合物具有良好的安全性。
功效性评价(紧密连接蛋白)：
请参阅图1和图2，采用免疫荧光法利用角质形成细胞测定紧密连接蛋白增量实验。首先进行MTT细胞毒性试验，得到最佳的样品测试浓度，参照该浓度进行下一步试验。以DMEM培养基和胎牛血清配置完全培养基，将复苏角质形成细胞接种于10cm培养盘，加入培养基后于二氧化碳培养箱孵育，后取形态良好的细胞支撑细胞悬液，接种于24孔板，于二氧化碳培养箱孵育24h后，加入经MTT试验验证的最佳浓度并稀释过的受试物即多维屏障修复组合物，并设置空白对照组，于二氧化碳培养箱中培养24h后，取上清液，通过免疫荧光法测定ZO-1和CLND1蛋白含量。细胞试验中ZO-1蛋白的生成量变化参见图1，CLND1蛋白的生成量变化参见图2，其中TJ05为使用多维屏障修复组合物实施例2样品溶液；BC组为空白对照；剩余组别为与本专利无关的其他样品。
功效性评价(角化包膜)：
请参阅图3、图4和图5，采用免疫荧光法法利用EpiKuits表皮模型测定角化包膜增量。将表皮模型转移至液体维持培养基中37℃稳定过夜培养18h，将表皮进行SLS处理形成化学敏感模型，然后将待测样品的多维屏障修复组合物溶液在模型上进行孵育24h，用DPBS将待测样品清洗，并用棉签将皮肤模型表面擦干，将皮肤模型用打孔器取下，分别用于切片组织石蜡包埋和RNA抽提，后者至Trizol溶液中-80℃条件下保存并进行后续蛋白含量测定。EpiKuits表皮模型试验中，FLG蛋白的生成量变化参见图3，LOR蛋白的生成量变化参见图4，TGM1蛋白的生成量变化参见图5。其中CE02为涂抹多维屏障修复组合物实施例2样品溶液后使用SLS进行刺激；BC组为空白对照；NC组为直接进行SLS刺激；PC组为使用SLS进行刺激后再使用地塞米松乳膏，作为阳性对照；SLS为十二烷基磺酸钠；剩余组别为与本专利无关的其他样品。
紧密连接蛋白和角化包膜测试实验结果显示，实施例2的多维屏障修复组合物样品能够显著促ZO-1、CLDN1和FLG、LOR、TGM1的生成，证明本方案具有促进紧密连接蛋白和角化包膜生成的功效。
综上所述，本发明的多维屏障修复组合物及其制备方法的技术方案，在功效性成分的基础上上增加了渗透平衡剂，使该组合物在屏障受损的皮肤上具有适宜的渗透力，能够很好地促进角化包膜和紧密连接蛋白表达、提升皮肤自主保湿力、强化皮肤脂质、调节免疫屏障并减轻皮肤炎症和平衡表皮微生态，对受损屏障全方位有针对性的进行修复，既不会对屏障受损的皮肤造成进一步的刺激，又能够全面有效地修复屏障，而且保证制剂安全温和可靠，同时该组合物在室温条件下保存时稳定性保持良好。
本技术领域中的普通技术人员应当认识到，以上的实施例仅是用来说明本发明，而并非用作为对本发明的限定，只要在本发明的实质精神范围内，对以上所述实施例的变化、变型都将落在本发明的权利要求书范围内。
Claims (8)
1.一种多维屏障修复组合物，其特征在于，包括以下重量份的组份：
功效复合物20～50份；
渗透平衡剂2～20份；
溶剂30～60份；
所述功效复合物包括：
功效成分A，能够促进角化包膜和紧密连接蛋白表达的成分；
功效成分B，能够提升皮肤自主保湿力的成分；
功效成分C，能够激活PPAR受体、强化皮肤脂质屏障的成分；
功效成分D，能够调节免疫屏障并减轻皮肤炎症的成分；
功效成分E，能够平衡表皮微生态的成分；
所述渗透平衡剂包括卵磷脂和/或氢化卵磷脂，所述渗透平衡剂维持所有功效成分安全有效地渗透至皮肤屏障的不同位点，使多维屏障修复组合物发挥功效；
所述溶剂包括去离子水和有机溶剂。
2.如权利要求1所述的一种多维屏障修复组合物，其特征在于，所述功效成分A、功效成分B、功效成分C、功效成分D、功效成分E各占功效复合物总重量的10～30％。
3.如权利要求1或2所述的一种多维屏障修复组合物，其特征在于，所述能够促进角化包膜和紧密连接蛋白表达的成分包括具距石枝藻提取物、红毛丹果皮提取物、麦冬提取物中的一种或多种；
所述能够提升皮肤自主保湿力的成分包括生物类黄酮、水解植物蛋白、长角豆籽提取物、水解三色堇提取物、低聚果糖中的一种或多种；
所述能够激活PPAR受体、强化皮肤脂质屏障的成分包括神经酰胺3、胆甾醇和油脂；
所述能够调节免疫屏障并减轻皮肤炎症的成分包括欧泽泻提取物、柑橘皮提取物、β-葡聚糖、玉兰花提取物、牡丹皮提取物、黄芩提取物、矢车菊花提取物中的一种或多种；
所述能够平衡表皮微生态的成分包括乳酸菌发酵溶胞产物、乙酰基七肽-4、酵母提取物、亚麻籽提取物、乳酸杆菌发酵溶胞产物中的一种或多种。
4.如权利要求3所述的一种多维屏障修复组合物，其特征在于，所述能够激活PPAR受体、强化皮肤脂质屏障的成分中，所述神经酰胺3、胆甾醇和油脂的重量比是9∶4∶0.5～9∶4∶2。
5.如权利要求4所述的一种多维屏障修复组合物，其特征在于，所述油脂为油酸、亚油酸、青刺果油、向日葵籽油、向日葵籽油不皂化物中的一种或多种。
6.如权利要求1所述的一种多维屏障修复组合物，其特征在于，所述有机溶剂为1,2-戊二醇、丁二醇和甘油中的一种或多种。
7.如权利要求1所述的一种多维屏障修复组合物，其特征在于，所述去离子水的重量占所述溶剂的总重量的5～20％。
8.如权利要求1所述的一种多维屏障修复组合物的制备方法，其特征在于，包括以下步骤：
S1，在称量好的有机溶剂中加入功效成分C，加热至70～75℃，搅拌至完全溶解，随后降温至40～45℃得到预配相1；
S2，将功效成分A、功效成分B、功效成分D、功效成分E以及去离子水常温混合并搅拌均匀，得到预配相2；
S3，将渗透平衡剂均匀铺展于固定平面，使其形成薄膜，缓慢注入预配相1和预配相2，旋转震荡使其水合，即得到所述多维屏障修复组合物。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110574579.2A CN113304086A (zh) | 2021-05-25 | 2021-05-25 | 一种多维屏障修复组合物及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110574579.2A CN113304086A (zh) | 2021-05-25 | 2021-05-25 | 一种多维屏障修复组合物及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113304086A true CN113304086A (zh) | 2021-08-27 |
Family
ID=77374723
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110574579.2A Pending CN113304086A (zh) | 2021-05-25 | 2021-05-25 | 一种多维屏障修复组合物及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113304086A (zh) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114028296A (zh) * | 2021-12-13 | 2022-02-11 | 上海宜侬生物科技有限公司 | 一个多维度修护皮肤敏感的化妆品成分与应用 |
| CN114931534A (zh) * | 2022-06-28 | 2022-08-23 | 上海新高姿化妆品有限公司 | 一种皮肤敏感修护组合物及其应用 |
| CN115607501A (zh) * | 2022-09-22 | 2023-01-17 | 广东科盈科技有限公司 | 乳酸菌修护精华乳、乳化装置及制备方法 |
| CN115645317A (zh) * | 2022-09-01 | 2023-01-31 | 杭州配方师科技有限公司 | 具有保湿功效的化妆品原料、用途及含该化妆原料的化妆品 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107951758A (zh) * | 2017-11-24 | 2018-04-24 | 广州赛莱拉干细胞科技股份有限公司 | 基于具距石枝藻提取物的修复防晒霜及修复防晒系统 |
| CN109498472A (zh) * | 2018-12-26 | 2019-03-22 | 广州蜜妆生物科技有限公司 | 一种柔润深层卸妆凝胶及其制备方法 |
| CN109715183A (zh) * | 2016-09-15 | 2019-05-03 | 巴斯夫美容护理法国公司 | 酵母提取物的新的美容化妆和/或营养保健或皮肤病学用途 |
| CN111214418A (zh) * | 2020-03-13 | 2020-06-02 | 佛山市奥姿美生物科技有限公司 | 高效屏障修复组合物及其应用 |
| CN111803414A (zh) * | 2020-07-13 | 2020-10-23 | 皓雨(广州)化妆品制造有限公司 | 一种用于皮肤屏障的组合物及其制备方法和应用 |
| CN112043667A (zh) * | 2020-09-12 | 2020-12-08 | 广州市爱百伊生物技术有限公司 | 一种保湿并维护皮肤屏障的组合物及其制备方法及其应用 |
| CN112137904A (zh) * | 2020-10-12 | 2020-12-29 | 四川荣乐化妆品有限公司 | 一种幻彩水光肌底精华液及其制备方法 |
-
2021
- 2021-05-25 CN CN202110574579.2A patent/CN113304086A/zh active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109715183A (zh) * | 2016-09-15 | 2019-05-03 | 巴斯夫美容护理法国公司 | 酵母提取物的新的美容化妆和/或营养保健或皮肤病学用途 |
| CN107951758A (zh) * | 2017-11-24 | 2018-04-24 | 广州赛莱拉干细胞科技股份有限公司 | 基于具距石枝藻提取物的修复防晒霜及修复防晒系统 |
| CN109498472A (zh) * | 2018-12-26 | 2019-03-22 | 广州蜜妆生物科技有限公司 | 一种柔润深层卸妆凝胶及其制备方法 |
| CN111214418A (zh) * | 2020-03-13 | 2020-06-02 | 佛山市奥姿美生物科技有限公司 | 高效屏障修复组合物及其应用 |
| CN111803414A (zh) * | 2020-07-13 | 2020-10-23 | 皓雨(广州)化妆品制造有限公司 | 一种用于皮肤屏障的组合物及其制备方法和应用 |
| CN112043667A (zh) * | 2020-09-12 | 2020-12-08 | 广州市爱百伊生物技术有限公司 | 一种保湿并维护皮肤屏障的组合物及其制备方法及其应用 |
| CN112137904A (zh) * | 2020-10-12 | 2020-12-29 | 四川荣乐化妆品有限公司 | 一种幻彩水光肌底精华液及其制备方法 |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114028296A (zh) * | 2021-12-13 | 2022-02-11 | 上海宜侬生物科技有限公司 | 一个多维度修护皮肤敏感的化妆品成分与应用 |
| CN114931534A (zh) * | 2022-06-28 | 2022-08-23 | 上海新高姿化妆品有限公司 | 一种皮肤敏感修护组合物及其应用 |
| CN115645317A (zh) * | 2022-09-01 | 2023-01-31 | 杭州配方师科技有限公司 | 具有保湿功效的化妆品原料、用途及含该化妆原料的化妆品 |
| CN115607501A (zh) * | 2022-09-22 | 2023-01-17 | 广东科盈科技有限公司 | 乳酸菌修护精华乳、乳化装置及制备方法 |
| CN115607501B (zh) * | 2022-09-22 | 2023-06-16 | 广东科盈科技有限公司 | 乳酸菌修护精华乳、乳化装置及制备方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111407719B (zh) | 一种皮肤屏障修护复合物、面霜及其制备方法 | |
| CN113304086A (zh) | 一种多维屏障修复组合物及其制备方法 | |
| CN109846757B (zh) | 一种皮脂仿生组合物及包含其的化妆品 | |
| CN105943491A (zh) | 一种润透保湿精华水及其制备方法 | |
| CN115317429B (zh) | 含重组胶原蛋白具有修护舒缓功效的组合物、含有该组合物的眼霜及制备方法和应用 | |
| CN106038385A (zh) | 一种刺激胶原蛋白生成抵抗皱纹的化妆品组合物 | |
| CN110755345A (zh) | 一种益生元组合物及其应用 | |
| US11045669B2 (en) | Hydroalcoholic extract of Schinus molle, cosmetic compositions comprising the same and cosmetic uses thereof | |
| CN110279602A (zh) | 皮肤修复组合物、其制备方法，以及在化妆品中的应用 | |
| CN114931530A (zh) | 一种具有抗敏和修复作用的护肤组合物及其应用和化妆品及其方法 | |
| CN113041172B (zh) | 用于肌肤屏障修护的皮脂膜仿生组合物及其应用和化妆品 | |
| KR20030023398A (ko) | 손바닥선인장 추출물을 함유하는 피부 외용제 조성물 | |
| CN113101256B (zh) | 一种抗衰修复纳米乳及其应用 | |
| FR3029417A1 (fr) | Complexe actif pour un produit cosmetique contre le vieillissement cutane | |
| CN111265454B (zh) | 一种保湿补水的抗衰老化妆品组合物 | |
| CN110801412B (zh) | 一种皮脂仿生组合物及其应用和化妆品 | |
| CN115518012B (zh) | 一种具有抑制黑色素蛋白、改善黑眼圈和眼袋功效的组合物及应用 | |
| CN116850081A (zh) | 一种用于敏感肌的修护组合物，冻干面膜及其制备方法 | |
| CN111202769A (zh) | 一种玫瑰痤疮用组合物、制剂及其制备方法 | |
| CN111358748A (zh) | 一种含海枣籽提取物的面膜精华液及其制备方法 | |
| CN115040448A (zh) | 一种强化抗老效果的护肤品及其制备方法 | |
| CN113143788A (zh) | 一种具有保湿修复舒缓的多效组合物及面霜 | |
| CN113171316A (zh) | 一种修复、缓解皮肤刺激化妆品及制备方法 | |
| KR102657790B1 (ko) | 천연추출물이 함유된 피부탄력 개선용 화장료 조성물 | |
| CN116869855B (zh) | 含有胶原蛋白的皮肤修护组合物 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210827 |