CN113295482B

CN113295482B - Full-automatic toxin extraction and detection integrated instrument

Info

Publication number: CN113295482B
Application number: CN202110365042.5A
Authority: CN
Inventors: 孙先佑; 马驰宇; 孔留兵
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2021-04-01
Filing date: 2021-04-01
Publication date: 2023-03-31
Anticipated expiration: 2041-04-01
Also published as: CN113295482A

Abstract

The invention discloses a full-automatic toxin extraction and detection integrated instrument, which comprises: installing a box body; a first mounting plate, a transportation support plate, a second mounting plate, a control distinguishing partition plate, a centrifugal distinguishing partition plate, a detection area partition plate and a peristaltic pump mounting plate are arranged in the mounting box body; the first mounting plate is provided with a conveying mechanism, a mincing station, a liquid adding and taking station and a homogenizing station; a control module for controlling the whole extraction and detection process is arranged in the control area; a centrifugal station for extracting a liquid to be detected from the mixture of the shellfish samples in the transportation mechanism is arranged in the centrifugal area; and a detection station for detecting the type and concentration of the toxin in the liquid to be detected extracted by the centrifugal station is arranged in the detection zone. The full-automatic toxin extraction and detection integrated instrument can automatically complete the grinding, extraction, homogenization, centrifugation, filtration and detection of shellfish samples, has a certain self-cleaning function, is simple and convenient to operate, and is suitable for rapid field detection.

Description

Full-automatic toxin extraction and detection integrated instrument

Technical Field

The invention relates to a marine organism sample pretreatment and toxin detection technology, in particular to an instrument capable of fully automatically completing sample pretreatment and toxin detection integration.

Background

With the increasing severity of marine pollution, marine toxins are firstly enriched in marine plants, some marine organisms such as shellfish organisms and the like can accumulate toxins in bodies due to ingestion of toxic plants, and if people eat the toxic organisms by mistake, poisoning symptoms can be caused, so that the life health of the people is threatened. The realization of the on-site rapid detection of the marine biotoxin is an effective means for preventing and treating marine biological pollution. Due to the complexity and the strong specialization of the shellfish toxin pretreatment and detection method, the shellfish toxin pretreatment and detection method can be only carried out in a laboratory in most cases, the time consumption is long, the efficiency is low, and the large-batch detection requirements cannot be met.

Disclosure of Invention

The invention provides a full-automatic toxin extraction and detection integrated instrument, which adopts the following technical scheme:

a full-automatic toxin extraction and detection integrated instrument comprises: installing a box body; a first mounting plate, a transportation support plate, a second mounting plate, a control distinguishing partition plate for isolating a control area, a centrifugal distinguishing partition plate for isolating a centrifugal area, a detection area partition plate for isolating a detection area and a peristaltic pump mounting plate for mounting a peristaltic pump are arranged in the mounting box body; the first mounting plate is provided with a transportation mechanism for transporting shellfish samples, a mincing station for mincing the shellfish samples in the transportation mechanism, a liquid adding and taking station for adding liquid or taking liquid into the transportation mechanism to react with the shellfish samples and a homogenizing station for homogenizing and stirring the shellfish samples in the transportation mechanism; a control module for controlling the whole extraction and detection process is arranged in the control area; a centrifugal station for extracting a liquid to be detected from the mixture of the shellfish samples in the transportation mechanism is arranged in the centrifugal area; a detection station for detecting the type and concentration of toxins in the liquid to be detected extracted by the centrifugal station is arranged in the detection zone; the peristaltic pump mounting plate is provided with a first micro peristaltic pump for providing extraction liquid, a second micro peristaltic pump for absorbing liquid to be detected, a third micro peristaltic pump for inputting the liquid to be detected at the centrifugal station into the detection station, a fourth micro peristaltic pump for inputting a reaction reagent into the detection station and a fifth micro peristaltic pump for providing cleaning liquid.

Further, the transportation mechanism comprises a chassis steering engine; the chassis steering engine is arranged on the first mounting plate; the chassis steering engine is connected with the bottom plate of the sample pool through a steering wheel and a steering wheel disc of the chassis steering engine; a thin film type pressure-sensitive sensor is arranged in the sample cell chassis; the film pressure-sensitive sensor can be provided with a pretreatment sample pool.

Furthermore, a positioning tongue is arranged at the bottom of the pretreatment sample pool; the sample Chi Depan is provided with a positioning port; the pretreatment sample pool is quickly arranged on the sample pool base plate through the positioning tongue and the positioning port.

Further, the mincing station comprises a first lead screw and a first optical axis; the upper part and the lower part of the first screw rod are respectively connected to the mounting box body through a first screw rod bearing and a second screw rod bearing; the upper part and the lower part of the first optical axis are respectively connected to the left plate of the machine shell through a first optical axis bearing and a second optical axis bearing; the lower part of the first lead screw is connected with a motor shaft of the first stepping motor through a first lead screw coupler; the first stepping motor is arranged on the first mounting plate; a first flange nut is arranged on the first lead screw; the first flange nut is connected with a first connecting beam; the first connecting beam is sleeved on the first optical axis through a first limiting hole; the tail end of the first connecting beam is connected with a first motor support; the first motor support is fixedly connected with a first direct current speed reducing motor and a first baffle cover; the first blocking cover is connected with a first horizontal bearing, and a meat grinding rod through hole is formed in the center of the first blocking cover; the motor shaft of the first direct current speed reduction motor is connected with a mincing coupler, the mincing coupler penetrates through the first horizontal bearing inner ring and the minced meat rod through hole, and the two ends of the mincing coupler are respectively connected with the motor shaft of the first direct current speed reduction motor and the minced meat rod for mincing the shellfish sample.

Furthermore, the first blocking cover is provided with 4 positioning clamping grooves; the transport mechanism is provided with 4 positioning keys which are used for being matched with the positioning clamping grooves.

Further, the liquid adding and taking station comprises a connecting rod bracket; the bottom of the connecting rod bracket is connected with the first mounting plate; the connecting rod bracket is provided with a steering engine mounting platform; the steering engine mounting table is connected with a micro steering engine; the left side and the right side of the connecting rod bracket are fixedly connected with a first fixing rod and a second fixing rod; a first transmission rod and a second transmission rod are connected between the first fixing rod and the second fixing rod through a pin shaft; the tail ends of the first transmission rod and the second transmission rod are connected with a first execution rod and a second execution rod; an extract liquid dropper, a cleaning liquid dropper and a pipette are connected between the first actuating rod and the second actuating rod; the extract liquid dropper is connected with the outlet of the first micro peristaltic pump through a Teflon pipe; the inlet of the first micro peristaltic pump is connected to the extraction liquid pool through a Teflon pipe; the cleaning liquid dropper is connected with the outlet of the fifth micro peristaltic pump through a Teflon pipe; the inlet of the fifth micro peristaltic pump is connected to the cleaning solution pool through a Teflon pipe; the liquid suction pipe is connected to the inlet of the second micro peristaltic pump through a Teflon pipe; the bottom of the extract liquid dropper is level with the bottom of the cleaning liquid dropper, and the bottom of the liquid sucker is 3cm lower than the bottom of the extract liquid dropper; and a reinforcing rib plate is arranged between the connecting rod bracket and the steering engine mounting platform.

Further, the homogenizing station comprises a second lead screw and a second optical axis; the upper part and the lower part of the second screw rod are respectively connected to the mounting box body through a third screw rod bearing and a fourth screw rod bearing; the upper part and the lower part of the second optical axis are respectively connected to the mounting box body through a third optical axis bearing and a fourth optical axis bearing; the lower part of the second screw rod is connected with a motor shaft of a second stepping motor through a second screw rod coupler; the second stepping motor is arranged on the first mounting plate; a second flange nut is arranged on the second lead screw and connected with a second connecting beam; the second connecting beam is sleeved on the second optical axis through a second limiting hole; the tail end of the second connecting beam is connected with a second motor support; the second motor support is fixedly connected with a second direct current speed reducing motor; and is connected with a second blocking cover; the second baffle cover is connected with a second horizontal bearing, and a stirring rod through hole is formed in the center of the second baffle cover; a motor shaft of the second direct current speed reducing motor is connected with a stirring coupler; the stirring coupler penetrates through the second horizontal bearing inner ring and the stirring rod through hole; a motor shaft of the second direct current speed reducing motor is connected with a stirring rod for stirring the shellfish sample through a stirring rod coupler; the second stop cover is provided with 4 positioning clamping grooves; the transportation mechanism is provided with 4 positioning keys which are used for being matched with the positioning clamping grooves; two vibration motor connecting rods are arranged below the second retaining cover; the center of the connecting rod of the vibration motor is provided with a through hole, and the lower part of the connecting rod of the vibration motor is connected with a waterproof vibration motor.

Further, the centrifugal station comprises a centrifugal direct current motor; the centrifugal direct current motor is fixed on the second mounting plate; a motor shaft of the centrifugal direct current motor is connected with a centrifugal mechanism through a centrifugal coupler; the centrifugal station also comprises a centrifugal sample adding screw rod and a centrifugal sampling screw rod; the centrifugal sample adding screw and the centrifugal sampling screw are both positioned above the centrifugal mechanism and are fixedly connected to the mounting box body through a first centrifugal screw fixing frame and a second centrifugal screw fixing frame respectively; a screw rod sliding table on the centrifugal sample-adding screw rod is connected with a centrifugal sample-adding needle tube; a screw rod sliding table of the centrifugal sampling screw rod is connected with a centrifugal sampling needle tube; the centrifugal sample-adding needle tube is connected to the outlet of the second micro peristaltic pump through a Teflon tube to absorb the liquid to be detected; the centrifugal sampling needle tube is connected to the inlet of the third micro peristaltic pump through a Teflon tube; the centrifugal station also comprises a filtering device; the filtering device comprises a first filtering cover and a second filtering cover; the first filter cover is connected to the outlet of the third micro peristaltic pump through a Teflon pipe; the second filter cover is connected to the detection station through a Teflon pipe so as to input the liquid to be detected into the detection station; a filter screen is arranged between the first filter cover and the second filter cover; the first filter cover is provided with the internal thread, and the second filter cover is provided with the external screw thread, and the two can close sealedly soon to make the filter screen fastening.

Further, the centrifugal mechanism also comprises a centrifugal support; a centrifugal test tube can be embedded in the centrifugal support; the centrifugal support is also provided with 4 support screw holes; the centrifugal mechanism further comprises a left buckle cover and a right buckle cover, and the left buckle cover and the right buckle cover are respectively provided with two buckle cover screw holes used for being matched with the support screw holes.

Furthermore, the centrifugal test tube is in a shape of , and the test tube opening of the centrifugal test tube is sealed with a layer of elastic sealing film.

Further, the detection station comprises a cuvette holder; the cuvette support is provided with a cuvette groove, an optical filter groove, a light source optical fiber interface and a spectrometer sensor optical fiber interface; a cuvette is placed in the cuvette groove; the optical filter can be placed in the optical filter groove; the light source optical fiber interface is connected with the LED light source through a light source optical fiber; the optical fiber interface of the spectrometer sensor is connected with the spectrometer sensor through the optical fiber of the spectrometer sensor; the spectrometer sensor is arranged on the control module; the detection station still includes: a first detection sample adding dropper for dropping a liquid to be detected and a second detection sample adding dropper for dropping a reaction reagent; the dropper ports of the first detection sample adding dropper and the second detection sample adding dropper are positioned above the cuvette; the first detection sample adding dropper is connected to the centrifugal station through a Teflon tube; the second detection sample adding dropper is connected to the outlet of the fourth micro peristaltic pump through a Teflon tube; the inlet of the fourth micro peristaltic pump is connected to the reaction reagent pool through a Teflon tube so as to suck the reaction reagent into the second detection sample adding dropper.

Furthermore, the mounting box body is provided with a first liquid pool fixing plate, a second liquid pool fixing plate, a third liquid pool fixing plate and a fourth liquid pool fixing plate; the first liquid pool fixing plate is fixedly connected with an extraction liquid pool; the second liquid pool fixing plate is fixedly connected with a reaction reagent pool; the third liquid pool fixing plate is fixedly connected with a cleaning liquid pool; the fourth liquid pool fixing plate is fixedly connected with a waste liquid pool; the extraction liquid pool, the reaction reagent pool and the cleaning liquid pool are also provided with Teflon pipe interfaces, and Teflon pipes extend to the bottom of the liquid pool.

Further, the installation box body is provided with a front door of the machine shell which is rotatably connected to the installation box body to close the installation box body; a front door handle and a display screen which is electrically connected with the control module to display operation options and detection results are arranged on the front door of the shell; a first permanent magnet is arranged on the inner side of the front door of the shell; the mounting box body is provided with a second permanent magnet; the front door of the machine shell is attracted to the installation box body through the first permanent magnet and the second permanent magnet to close the installation box body.

Further, the top of the installation box body is connected with a top handle and is provided with a switch sliding cover; the switch sliding cover is connected to the top of the installation box body in a sliding mode and fixed through the positioning pin.

The full-automatic toxin extraction and detection integrated instrument has the beneficial effects that the grinding, extraction, homogenization, centrifugation, filtration and detection of shellfish samples can be completed automatically, and the instrument has a certain self-cleaning function. The operation is simple and convenient, the operation personnel is not required to have a professional toxin detection background, and the user range is wide. On the premise of ensuring the automatic function, the invention also has the characteristics of small volume, light weight and convenient carrying, is suitable for on-site rapid detection, and greatly improves the pretreatment of shellfish samples and the toxin detection efficiency. The invention is not only suitable for extracting and detecting the shellfish toxin, but also can be used for detecting samples such as shrimps, fishes and the like as long as the samples accord with the pretreatment and detection paradigm of the invention, has the characteristic of wide application range, has higher application value and has huge potential.

Drawings

FIG. 1 is a schematic diagram of the fully automated toxin extraction and detection integrated instrument of the present invention;

FIG. 2 is a schematic view of another perspective of the fully automated toxin extraction and detection integrated instrument of FIG. 1;

FIG. 3 is a schematic view of the internal structure of the fully automated toxin extraction and detection integrated instrument of FIG. 1 with portions broken away;

FIG. 4 is a schematic diagram of the internal structure of the fully automated toxin extraction and detection integrated instrument of FIG. 1 with further portions broken away;

FIG. 5 is a schematic view of the transport mechanism of the fully automated toxin extraction and detection integrated instrument of FIG. 3;

FIG. 6 is a schematic diagram of the mincing station of the fully automated toxin extraction and detection integrated instrument of FIG. 3;

FIG. 7 is a schematic view of a liquid adding and extracting station of the fully automated toxin extraction and detection integrated instrument of FIG. 3;

FIG. 8 is a schematic view of a homogenization station of the fully automated toxin extraction and detection integrated instrument of FIG. 3;

FIG. 9 is a schematic diagram of a centrifuge station of the fully automated toxin extraction and detection integrated instrument of FIG. 4;

FIG. 10 is a schematic view of the filter assembly of the centrifuge station of the fully automated toxin extraction and detection integrated instrument of FIG. 4;

FIG. 11 is a schematic view of a detection station of the fully automated toxin extraction and detection integrated instrument of FIG. 4;

the full-automatic toxin extracting and detecting integrated instrument 1 comprises an installation box body 10, a machine shell bottom plate 101, a machine shell front door 102, a display screen 103, a machine shell rear plate 104, a machine shell left plate 105, a machine shell right plate 106, a first installation plate 107, a transportation support plate 108, a second installation plate 109, a control division partition plate 110, a centrifugal division partition plate 111, a detection zone partition plate 112, a peristaltic pump installation plate 113, a machine shell top plate 114, a control button 115, a rubber base 116, a front door handle (not shown), a first permanent magnet 118, a second permanent magnet 119, a first liquid pool fixing plate 120, a second liquid pool fixing plate 121, a third liquid pool fixing plate 122, a fourth liquid pool fixing plate 123, an extraction liquid pool 124, a reaction reagent pool 125, a cleaning liquid pool 126, a waste liquid pool 127, a Teflon pipe interface 128, a top plate 129, a switch sliding cover 130, a positioning pin 131, a transportation mechanism 20 and a steering engine chassis 201, a chassis steering engine steering wheel 202, a sample Chi Depan, a positioning port 204, a thin film pressure-sensitive sensor (not shown), a pretreatment sample pool 206, a positioning tongue 207, a positioning key 208, a mincing station 30, a first lead screw 301, a first optical axis 302, a first lead screw bearing 303, a second lead screw bearing 304, a first optical axis bearing 305, a second optical axis bearing 306, a first lead screw coupler 307, a first stepping motor 308, a first flange nut 309, a first connecting beam 310, a first limiting hole 311, a first motor support 312, a first direct current speed reducing motor (not shown), a first baffle cover 314, a positioning clamping groove 315, a first horizontal bearing 316, a minced meat rod through hole 317, a minced meat coupler 318, a minced meat rod 319, a liquid adding and taking station 40, a connecting rod support 401, a steering engine mounting platform 402, a micro steering engine 403, a first fixing rod 404, a second fixing rod 405, a first transmission rod 406, a second transmission rod 407, a first actuating rod 408, a second actuating rod 409, an extraction liquid dropper 410, a cleaning liquid dropper 411, a liquid suction pipe 412, a reinforcing rib plate (not shown), a first actuating rod long end 414, a first actuating rod short end 415, a second actuating rod long end 416, a second actuating rod short end 417, a homogenization station 50, a second lead screw 501, a second optical axis 502, a third lead screw bearing 503, a fourth lead screw bearing 504, a third optical axis bearing 505, a fourth optical axis bearing 506, a second lead screw coupler 507, a second stepping motor 508, a second flange nut 510, a second connecting beam 510, a second limiting hole 511, a second motor support 512, a second direct current speed reducing motor (not shown), a second blocking cover 514, a second horizontal bearing 515, a stirring rod through hole 516, a stirring rod vibrating coupler 517, a stirring rod 518, a connecting rod 519, a waterproof vibrating motor 520, a centrifugation station 60, a centrifugal DC motor 601, a centrifugal coupling 602, a centrifugal mechanism 603, a centrifugal sample-adding screw 604, a centrifugal sample-adding screw 605, a first centrifugal screw holder 606, a second centrifugal screw holder 607, a centrifugal sample-adding needle tube 608, a centrifugal sample-adding needle tube 609, a centrifugal support 610, a centrifugal test tube (not shown), a support screw hole 612, a left buckle cover 613, a right buckle cover 614, a buckle cover screw hole 615, an elastic sealing film 616, a filter 617, a first filter cover 6171, a second filter cover 6172, a filter screen 6173, a detection station 70, a cuvette support 701, a cuvette groove 702, a filter plate groove 703, a light source optical fiber interface 704, a spectrometer sensor optical fiber interface 705, a cuvette 706, a first detection sample-adding dropper 707, a second detection sample-adding dropper 708, a control module 80, a first micro peristaltic pump 901, a second micro peristaltic pump, a third micro pump 902, a fourth micro peristaltic pump 904 and a fifth micro peristaltic pump 905.

Detailed description of the preferred embodiments

The invention is described in detail below with reference to the figures and the embodiments.

As shown in fig. 1 to 11, a fully automatic toxin extraction and detection integrated instrument 1 according to the present invention includes a housing bottom plate 101, a housing front door 102, a display screen 103, a housing rear plate 104, a housing left plate 105, a housing right plate 106, a first mounting plate 107, a transportation support plate 108, a second mounting plate 109, a control division plate 110, a centrifugal division plate 111, a detection area division plate 112, a peristaltic pump mounting plate 113, and a housing top plate 114, which are all connected by welding. The display screen 103 is glued above the front door 102 of the casing, the front door 102 of the casing is connected with the left plate 105 of the casing through the first louver and the second louver, the top plate 114 of the casing is provided with control keys 115 for controlling the opening and closing and the operation of the instrument, and 4 rubber bases 116 are arranged below the bottom plate 101 of the casing.

The front door 102 of the cabinet is provided with a front door handle, the right side of the front door handle is provided with a first permanent magnet 118, the right plate 106 of the cabinet is provided with a second permanent magnet 119, and the first permanent magnet 118 and the second permanent magnet 119 can attract each other. Thus, the front door handle is convenient to open and shellfish samples are added.

The rear housing plate 104 is provided with a first liquid pool fixing plate 120, a second liquid pool fixing plate 121, a third liquid pool fixing plate 122 and a fourth liquid pool fixing plate 123, the first liquid pool fixing plate 120 is fixedly connected with an extraction liquid pool 124, the second liquid pool fixing plate 121 is fixedly connected with a reaction reagent pool 125, the third liquid pool fixing plate 122 is fixedly connected with a cleaning liquid pool 126, and the fourth liquid pool fixing plate 123 is fixedly connected with a waste liquid pool 127. The extraction liquid pool 124, the reaction reagent pool 125, the cleaning liquid pool 126 and the waste liquid pool 127 are all provided with threaded openings, the threaded openings can be closed by screwing a liquid pool cover provided with internal threads, the extraction liquid pool 124, the reaction reagent pool 125 and the cleaning liquid pool 126 are also provided with Teflon pipe interfaces 128, and Teflon pipes extend to the bottom of the liquid pools. Thus, each liquid pool is arranged outside the shell, and the required solution is convenient to supplement. The Teflon tube extends to the bottom of the liquid pool through the Teflon tube interface 128, and the utilization rate of the volume of the liquid pool is ensured.

Transport mechanism 20 is installed to first mounting panel 107, installs on casing left side board 105 and the first mounting panel 107 and rubs station 30, and liquid feeding and liquid station 40 of getting is installed to first mounting panel 107, installs homogeneity station 50 on casing back plate 104 and the first installation board, installs centrifugal station 60 on second mounting panel 109 and the casing roof 114, and the detection station 70 is installed to centrifugal partition board 111 and the second mounting panel 109 of distinguishing, and 5 miniature peristaltic pumps are installed to peristaltic pump mounting panel 113. The 5 micro peristaltic pumps are respectively a first micro peristaltic pump 901 for providing extraction liquid, a second micro peristaltic pump 902 for absorbing liquid to be detected, a third micro peristaltic pump 903, a fourth micro peristaltic pump 904 and a fifth micro peristaltic pump 905 for providing cleaning liquid.

The transportation mechanism 20 comprises a chassis steering engine 201, the chassis steering engine 201 is fixed on the first mounting plate 107 through bolts and nuts, the chassis steering engine 201 is connected with a sample cell chassis 203 through a chassis steering engine steering wheel 202, a thin film type pressure-sensitive sensor 205 is arranged in the sample cell chassis 203, and a pretreatment sample cell 206 can be arranged on the thin film type pressure-sensitive sensor 205. A thin film pressure sensitive sensor 205 is positioned between the sample cell chassis 203 and the pre-treatment sample cell 206. The thin film pressure-sensitive sensor 205 can convert the pressure value received into an electric signal and transmit the electric signal to the single chip microcomputer, and the single chip microcomputer can calculate the weight change in the pretreatment sample pool 206 according to the difference value of the electric signals twice in sequence to be used as control feedback. When the shellfish meat sample is added, when the weight of the shellfish meat sample reaches a preset value, the singlechip, namely the control instrument, performs the next action, and the film type pressure-sensitive sensor 205 serves as a feedback link when the extraction liquid is dripped and the homogeneous liquid is sucked.

The pretreatment sample pool 206 is provided with 4 positioning keys 208, the bottom of the pretreatment sample pool 206 is provided with a positioning tongue 207, the sample Chi Depan is provided with a positioning port 204, and the positioning tongue 207 is clamped in the positioning port 204. After the positioning tongue 207 is tightly clamped with the positioning port 204, the movement of the pretreatment sample cell 206 in the working process of the instrument can be prevented.

The mincing station 30 comprises a first lead screw 301 and a first optical axis 302, the upper part and the lower part of the first lead screw 301 are respectively connected to the left casing plate 105 through a first lead screw bearing 303 and a second lead screw bearing 304, the upper part and the lower part of the first optical axis 302 are respectively connected to the left casing plate 105 through a first optical axis bearing 305 and a second optical axis bearing 306, and the first lead screw bearing 303, the second lead screw bearing 304, the first optical axis bearing 305 and the second optical axis bearing 306 are all connected to the left casing plate 105 through bolts and nuts. The lower part of the first lead screw 301 is connected with the motor shaft of a first stepping motor 308 through a first lead screw coupler 307, and the first stepping motor 308 is mounted on the first mounting plate 107 through bolts. First flange nut 309 is installed on first lead screw 301, first flange nut 309 has first coupling beam 310 through bolted connection, first coupling beam 310 overlaps in first optical axis 302 through first spacing hole 311, first coupling beam 310 is terminal to have first motor support 312 through bolted connection, first motor support 312 has first direct current gear motor through the bolt fastening, first direct current gear motor is located first motor support 312, not shown in the figure. And is connected with first fender lid 314 through 4 hexagonal studs, and first fender lid 314 is opened has 4 positioning slot 315, and first fender lid 314 passes through bolted connection has first horizontal bearing 316, and opens at the center of first fender lid 314 has ground meat stick through-hole 317, and ground meat stick through-hole 317 is concentric with first horizontal bearing 316 inner circle. The motor shaft of the first direct current speed reduction motor is connected with a mincing coupler 318, the mincing coupler 318 penetrates through the inner ring of the first horizontal bearing 316 and the minced meat rod through hole 317, and the motor shaft of the first direct current speed reduction motor is connected with the minced meat rod 319 through a minced meat rod 319 coupler.

Liquid feeding and liquid taking station 40 includes connecting rod support 401, and connecting rod support 401 bottom is through bolted connection in first mounting panel 107, and connecting rod support 401 is provided with steering wheel mount table 402. The steering engine mounting table 402 is connected with a micro steering engine 403 through bolts. The left side and the right side of the connecting rod bracket 401 are fixedly connected with a first fixing rod 404 and a second fixing rod 405 through bolts, a first transmission rod 406 and a second transmission rod 407 are connected between the first fixing rod 404 and the second fixing rod 405 through pin shafts, the first transmission rod 406 is parallel to the second transmission rod 407, and the tail ends of the first transmission rod 406 and the second transmission rod 407 are connected with a first execution rod 408 and a second execution rod 409 through pin shafts. The first actuating rod 408 may be subdivided into a first actuating rod long end 414 and a first actuating rod short end 415, the second actuating rod 409 may be subdivided into a second actuating rod long end 416 and a second actuating rod short end 417, a liquid pick-up tube 412 may be connected between the first actuating rod long end 414 and the second actuating rod long end 416, and an extract pick-up tube 410 and a cleaning liquid pick-up tube 411 may be connected between the first actuating rod short end 415 and the second actuating rod short end 417. The bottom of extraction liquid dropper 410 is level with the bottom of cleaning liquid dropper 411, and the bottom of pipette 412 is 3cm lower than the bottom of extraction liquid dropper 410. The beneficial effect is that the bottom of the liquid suction pipe 412 is lower than the bottoms of the extraction liquid dropper 410 and the cleaning liquid dropper 411, so that the extraction liquid dropper 410 and the cleaning liquid dropper 411 do not touch the liquid level in the pretreatment sample pool 206 when the mechanism sucks liquid, and the extraction liquid dropper 410 and the cleaning liquid dropper 411 can be prevented from being polluted by samples.

An extract liquid dropper 410 is connected to the outlet of the first micro peristaltic pump 901 through a Teflon pipe, the inlet of the first micro peristaltic pump 901 is connected to the extract liquid pool 124 through a Teflon pipe, a cleaning liquid dropper 411 is connected to the outlet of the fifth micro peristaltic pump 905 through a Teflon pipe, the inlet of the fifth micro peristaltic pump 905 is connected to the cleaning liquid pool 126 through a Teflon pipe, and a liquid suction pipe 412 is connected to the inlet of the second micro peristaltic pump 902 through a Teflon pipe.

A reinforcing rib plate is arranged between the connecting rod bracket 401 and the steering engine mounting platform 402. One end of the reinforcing rib plate is connected with the connecting rod bracket 401, and the other end is connected with the lower side of the steering engine mounting table 402 so as to support the steering engine mounting table 402 from the lower side of the steering engine mounting table 402. The first transmission lever 406 is kept parallel to the second transmission lever 407. The reinforcing rib plate has the beneficial effects that the vibration of the connecting rod bracket 401 during working can be reduced, and the stability of the system is improved; the first transmission rod 406 and the second transmission rod 407 are parallel, so that the fixed rods (the first fixed rod 404 and the second fixed rod 405), the first transmission rod 406, the second transmission rod 407 and the actuating rods (the first actuating rod 408 and the second actuating rod 409) form a parallelogram four-bar linkage mechanism, so that the actuating rods are always vertical to complete the liquid feeding and absorbing process.

The homogenizing station 50 includes a second lead screw 501 and a second optical axis 502, the upper portion and the lower portion of the second lead screw 501 are respectively connected to the housing back plate 104 through a third lead screw bearing 503 and a fourth lead screw bearing 504, the upper portion and the lower portion of the second optical axis 502 are respectively connected to the housing back plate 104 through a third optical axis bearing 505 and a fourth optical axis bearing 506, and the third lead screw bearing 503, the fourth lead screw bearing 504, the third optical axis bearing 505 and the fourth optical axis bearing 506 are all connected to the housing back plate 104 through bolts and nuts. The lower part of the second screw 501 is connected with the motor shaft of a second stepping motor 508 through a second screw coupling 507, and the second stepping motor 508 is mounted on the first mounting plate 107 through bolts. A second flange nut 509 is mounted on the second lead screw 501, the second flange nut 509 is connected with a second connecting beam 510 through a bolt, the second connecting beam 510 is sleeved on the second optical axis 502 through a second limiting hole 511, the tail end of the second connecting beam 510 is connected with a second motor support 512 through a bolt, the second motor support 512 is fixedly connected with a second direct current speed reduction motor through a bolt, and the second direct current speed reduction motor is located in the second motor support 512 and is not shown in the drawing. And is connected with a second stopper 514 through 4 hex studs. The second baffle cover 514 is connected with a second horizontal bearing 515 through bolts, a stirring rod through hole 516 is formed in the center of the second baffle cover 514, and the stirring rod through hole 516 is concentric with the inner ring of the second horizontal bearing 515. The motor shaft of the second direct current gear motor is connected with a stirring coupler 517, the stirring coupler 517 penetrates through the inner ring of the second horizontal bearing 515 and the stirring rod through hole 516, and the motor shaft of the second direct current gear motor is connected with the stirring rod 518 through a stirring rod 518 coupler.

The first blocking cover 314 and the second blocking cover 514 are respectively provided with 4 positioning card slots 315, and the pretreatment sample cell 206 is provided with 4 positioning keys 208. The beneficial effect that the positioning slot 315 is matched with the positioning key 208 to prevent the movement of the pretreatment sample cell 206 during the operation of the apparatus. Two vibration motor connecting rods 519 are arranged below the second blocking cover 514, a through hole is formed in the center of each vibration motor connecting rod 519, and the lower portion of each vibration motor connecting rod 519 is connected with a waterproof vibration motor 520. The beneficial effect that produces is that, homogenization station 50 not only has the stirring effect of stirring rod 518 when the homogeneity, still has the vibration effect of waterproof shock dynamo 520, can strengthen the homogeneity effect or improve homogeneity efficiency.

The centrifugal station 60 includes a centrifugal dc motor 601, the centrifugal dc motor 601 is fixed on the second mounting plate 109 by bolts, and a motor shaft of the centrifugal dc motor 601 is connected to a centrifugal mechanism 603 by a centrifugal coupling 602. The centrifugal mechanism 603 includes a centrifugal support 610, a centrifugal test tube can be embedded in the centrifugal support 610, the centrifugal test tube is in a shape of , and a test tube port of the centrifugal test tube is sealed with a layer of elastic sealing film 616. The centrifugal test tube is symmetrical about the axis of the centrifugal direct current motor 601, eccentricity caused by asymmetric load on the centrifugal direct current motor 601 can be avoided in the high-speed centrifugation process, and meanwhile, the tube opening of the centrifugal test tube is kept horizontal, so that automatic sample introduction and sampling are facilitated. The tube mouth of the centrifugal test tube is sealed with an elastic sealing film 616, so that the sealing of the centrifugal test tube in the centrifugal process can be ensured, and the centrifugal sample injection needle tube 608 and the centrifugal sample injection needle tube 609 can conveniently puncture the film to realize sample injection and sampling. (the working process of the station is sample adding-centrifuging-sampling, after the centrifugal sample adding needle tube 608 punctures the elastic sealing film 616, the film is pulled out, and the film still has sealing function, so that the liquid can not be splashed out in the centrifuging process). The eccentric holder 610 is also provided with 4 holder screw holes 612. The centrifugal mechanism 603 further includes a left cover 613 and a right cover 614, and the left cover 613 and the right cover 614 are respectively provided with two cover screw holes 615. The support screw hole 612 is connected with the cover screw hole 615 through four bolts. Because the centrifugal test tube is disposable, need artifical the change after detecting at every turn, through four bolted connection between support screw 612 and buckle closure screw 615, can realize the quick replacement of centrifugal test tube through the mode of screwing on and unscrewing the bolt. The centrifugal station 60 further comprises a centrifugal sample adding lead screw 604 and a centrifugal sample adding lead screw 605, wherein the centrifugal sample adding lead screw 604 and the centrifugal sample adding lead screw 605 are both located above the centrifugal mechanism 603 and fixedly connected with the top plate 114 of the machine shell through a first centrifugal lead screw fixing frame 606 in a bolt fixing manner, and a second centrifugal lead screw fixing frame 607 is fixedly connected with the top plate 114 of the machine shell, a lead screw sliding table on the centrifugal sample adding lead screw 604 is connected with a centrifugal sample adding needle tube 608, the lead screw sliding table on the centrifugal sample adding lead screw 605 is connected with a centrifugal sample adding needle tube 609, the centrifugal sample adding needle tube 608 is connected with an outlet of the second micro peristaltic pump 902 through a Teflon tube, and the centrifugal sample adding needle tube 609 is connected with an inlet of the third micro peristaltic pump 903 through a Teflon tube. The centrifuge station 60 also includes a filter device 617. The filter unit 617 is mounted on the centrifuge separator plate within the centrifuge zone. The filtering device 617 includes a first filtering cover 6171 and a second filtering cover 6172, the first filtering cover 6171 is connected to the outlet of the third micro peristaltic pump 903 through a teflon tube, the second filtering cover 6172 is connected to the first detecting sample-adding dropper 707 through a teflon tube, a filtering net 6173 is arranged between the first filtering cover 6171 and the second filtering cover 6172, the first filtering cover 6171 is provided with an internal thread, the second filtering cover 6172 is provided with an external thread, the two can be screwed and sealed, and the filtering net 6173 is fastened.

The detection station 70 includes a cuvette holder 701, and the cuvette holder 701 is provided with a cuvette groove 702, a filter groove 703, a light source fiber interface 704, and a spectrometer sensor fiber interface 705. A cuvette 706 is placed in the cuvette groove 702, an optical filter can be placed in the optical filter groove 703, the light source optical fiber interface 704 is connected to the LED light source through a light source optical fiber, the spectrometer sensor optical fiber interface 705 is connected to the spectrometer sensor through a spectrometer sensor optical fiber, and the spectrometer sensor is installed in the control module 80. The detection station 70 further comprises a first detection sample adding dropper 707 and a second detection sample adding dropper 708, the dropper ports of the first detection sample adding dropper 707 and the second detection sample adding dropper 708 are located above the cuvette 706, the second detection sample adding dropper 708 is connected to the outlet of the fourth micro peristaltic pump 904 through a teflon tube, and the inlet of the fourth micro peristaltic pump 904 is connected to the reaction reagent cell 125 through a teflon tube.

In the detection process of the detection station 70, the first detection sample adding dropper 707 firstly drops the pretreated liquid to be detected into the cuvette 706, the second detection sample adding dropper 708 then drops the reaction reagent into the cuvette 706, after the reaction is carried out for a while, the LED light source emits a light signal which is irradiated onto the cuvette 706 through the light source optical fiber, the light signal is received by the spectrometer sensor after passing through the cuvette 706 and the solution therein and is converted into an electric signal which is transmitted to the detection circuit board for processing, the detection circuit board transmits the processed electric signal to the single chip microcomputer, the single chip microcomputer can obtain the absorbance value of the liquid to be detected at a specific wavelength after calculation, and can obtain the type and concentration of the toxin in the liquid to be detected after comparison with the standard, thereby completing the toxin detection.

The top plate handle 129 is connected to the top plate 114 of the housing, the top plate 114 of the housing is provided with a switch sliding cover 130, the switch sliding cover 130 is slidably connected to the top plate 114 of the housing, and the switch sliding cover 130 can be slidably connected to the top plate 114 of the housing through a sliding groove and then fixed by a positioning pin 131. This facilitates opening the slide cover 130 after each test is completed, and replacing the disposable centrifuge tube and the disposable cuvette 706.

The working process of the invention is as follows:

the extraction liquid tank 124, the reaction reagent tank 125 and the cleaning liquid tank 126 are filled with extraction liquid, reaction reagent and cleaning liquid, respectively. And (5) peeling the shellfish sample to be detected, and preparing the shellfish meat sample. Pressing a starting button, after the instrument is started, displaying options such as the type, toxin type, pretreatment mode, detection mode and the like of a sample to be processed on the display screen 103, selecting the corresponding option through the upturning button, the downturning button, the selection button and the like on the display screen 103, finally pressing the selection button, and opening the front door 102 of the shell of the instrument.

The pretreatment sample pool 206 is driven by the chassis steering engine 201 to screw out, a shellfish sample is added, the thin film pressure-sensitive sensor 205 converts the sensed pressure increasing signal into an electric signal and transmits the electric signal to the single chip microcomputer in the control module 80, and when the current change reaches a set value in the mode, the instrument reminds through light and stops adding the sample continuously.

First, the pretreatment sample tank 206 is rotated to a position right below the mincing station 30, and at this time, the first stepper motor 308 drives the first lead screw 301 to rotate forward through the first lead screw coupler 307, and drives the mincing mechanism to descend through the first flange nut 309, so that the first blocking cover 314 and the pretreatment sample tank 206 are clamped. The first dc gear motor then rotates the meat grinder 319 via the grinding coupling 318 to grind the shell meat sample into pieces. After the time requirement under the mode is met, the first direct current speed reducing motor stops rotating, the first stepping motor 308 drives the first lead screw 301 to rotate reversely through the first lead screw coupler 307, and the mincing mechanism is driven to ascend through the first flange nut 309 until the mincing mechanism is reset.

Then, the pretreatment sample pool 206 is rotated to the position below the liquid adding and taking station 40, at this time, the micro steering engine 403 acts to drive the second transmission rod 407 to rotate clockwise, so that the extract liquid dropper 410 is positioned above the pretreatment sample pool 206, then the first micro peristaltic pump 901 acts to pump the extract liquid in the extract liquid pool 124 into the pretreatment sample pool 206 to mix with the minced shellfish meat sample, the thin film pressure-sensitive sensor 205 converts the sensed pressure increase signal into an electric signal and transmits the electric signal to the single chip microcomputer, when the current change amount reaches the set value in the mode, the first micro peristaltic pump 901 stops working, and the micro steering engine 403 drives the second transmission rod 407 to rotate counterclockwise until the reset is performed.

Then, the pretreatment sample cell 206 is rotated to a position right below the homogenization station 50, and at this time, the second stepper motor 508 drives the second lead screw 501 to rotate forward through the second lead screw coupler 507, and drives the homogenization mechanism to descend through the second flange nut 509, so that the second stopper 514 and the pretreatment sample cell 206 are clamped tightly. Then the second direct current gear motor drives the stirring rod 518 to rotate through the stirring coupler 517, the two waterproof vibration motors 520 work to fully stir and vibrate the shellfish meat sample and the extraction liquid, after the time requirement under the mode is met, the second direct current gear motor and the two waterproof vibration motors 520 stop working, the second stepping motor 508 drives the second lead screw 501 to reversely rotate through the second lead screw coupler 507, and drives the homogenizing mechanism to ascend through the second flange nut 509 until resetting.

Then, the pretreatment sample pool 206 is rotated to the lower part of the liquid adding and taking station 40, at this time, the micro steering engine 403 acts to drive the second transmission rod 407 to rotate clockwise, so that the pipette 412 extends to the position below the liquid level of the homogenized mixed liquid, meanwhile, the centrifugal sample adding lead screw 604 in the centrifugal station 60 acts to drive the centrifugal sample adding needle tube 608 to descend and extend into the centrifugal test tube, then the second micro peristaltic pump 902 acts to transport the mixed liquid in the pretreatment sample pool 206 to the centrifugal test tube through the teflon tube and the centrifugal sample adding needle tube 608, the thin film pressure-sensitive sensor 205 converts the sensed pressure-reduced signal into an electric signal and transmits the electric signal to the single chip microcomputer, when the current change reaches the set value in the mode, the second micro peristaltic pump 902 stops working, and the micro steering engine 403 drives the second transmission rod 407 to rotate counterclockwise until resetting.

Next, the centrifugal sample adding screw 604 in the centrifugal station 60 acts to drive the centrifugal sample adding needle tube 608 to rise until it is reset, the centrifugal dc motor 601 drives the centrifugal test tube to rotate according to the speed required by the mode through the centrifugal coupler 602, because the elastic sealing film 616 at the tube opening of the centrifugal test tube has elasticity, and the pin hole left by the centrifugal sample adding needle tube 608 on the elastic sealing film 616 is very small, and the liquid in the centrifugal process is subjected to a centrifugal force in the horizontal direction, so that the liquid does not spill out of the centrifugal test tube. When the time requirement under the mode is met, the centrifugal direct current motor 601 stops rotating, the centrifugal sampling screw 605 acts to drive the centrifugal sampling needle tube 609 to descend to a position below the liquid level of the centrifuged supernatant, the third micro peristaltic pump 903 acts to suck the supernatant into the filtering device 617, the supernatant is filtered by the filter screen 6173 and then is dripped into the cuvette 706 in the cuvette support 701 through the first detection sample adding dropper 707, meanwhile, the fourth micro peristaltic pump 904 acts to suck the reaction reagent in the reaction reagent pool 125 into the cuvette 706, and after the volume requirement on the reactant under the mode is met, the third micro peristaltic pump 903 and the fourth micro peristaltic pump 904 stop acting. After the requirement of the reaction time in the mode is met, the LED light source emits light signals, the light signals irradiate the cuvette 706 through the light source optical fibers, the light signals are received by the spectrometer sensor after passing through the cuvette 706 and the solution in the cuvette 706 and are converted into electric signals to be transmitted to the detection circuit board for processing, the detection circuit board transmits the processed electric signals to the single chip microcomputer, the single chip microcomputer can obtain the absorbance value of the liquid to be detected at a specific wavelength after calculation, the absorbance value is compared with a standard to obtain the type and the concentration of toxins in the liquid to be detected, and toxin detection is completed.

And finally, displaying the detection result and the process parameters on the screen, pressing a selection key to store the data, and finally selecting a detection completion option. At this time, the positioning pin 131 between the top plate 114 of the housing and the switch sliding cover 130 is pulled out, and the switch sliding cover 130 is slid out, so as to replace the new centrifugal test tube and cuvette 706. After the work is finished, the sliding cover 130 is slid back and the positioning pin 131 is installed. The front door 102 of the cabinet is opened and the cleaning option is selected, the pretreatment reservoir 206 is automatically unscrewed, the reservoir is removed and the remaining waste solution is poured into the waste reservoir 127 and reinstalled. When the film pressure-sensitive sensor 205 senses that the pretreatment sample pool 206 is remounted, the film pressure-sensitive sensor automatically rotates to the position below the liquid adding and taking station 40, the micro steering engine 403 acts to drive the second transmission rod 407 to rotate clockwise, so that the cleaning liquid dropper 411 is positioned above the pretreatment sample pool 206, then the fifth micro peristaltic pump 905 acts to pump the cleaning liquid in the cleaning liquid pool 126 into the pretreatment sample pool 206, after the cleaning liquid reaches a specified amount, the fifth micro peristaltic pump 905 stops acting, and the micro steering engine 403 drives the second transmission rod 407 to rotate anticlockwise until the second transmission rod is reset. Subsequently, the pretreatment sample cell 206 is rotated to the mincing station 30 and the homogenizing station 50, and the mincing and homogenizing actions are repeated to clean the mincing mechanism and the homogenizing mechanism. After the cleaning is completed, the pretreatment sample tank 206 is manually taken out, and the waste liquid is poured into the waste liquid tank 127.

The invention has the beneficial effects that the full-automatic toxin extraction and detection integrated instrument 1 is designed and realized, the grinding, extraction, homogenization, centrifugation, filtration and detection of shellfish samples can be completed automatically, and the automatic toxin extraction and detection integrated instrument has a certain self-cleaning function. The operation is simple and convenient, the operator is not required to have a professional toxin detection background, and the user range is wide. On the premise of ensuring the automatic function, the invention also has the characteristics of small volume, light weight and convenient carrying, is suitable for on-site rapid detection, and greatly improves the pretreatment of shellfish samples and the toxin detection efficiency. The invention is not only suitable for extracting and detecting the shellfish toxin, but also can be used for detecting samples such as shrimps, fishes and the like as long as the samples accord with the pretreatment and detection paradigm of the invention, has the characteristic of wide application range, has higher application value and has huge potential.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.

Claims

1. A full-automatic toxin extraction and detection integrated instrument comprises: installing a box body; the device is characterized in that a first mounting plate, a transportation support plate, a second mounting plate, a control distinguishing partition plate for separating a control area, a centrifugal distinguishing partition plate for separating a centrifugal area, a detection area partition plate for separating a detection area and a peristaltic pump mounting plate for mounting a peristaltic pump are arranged in the mounting box body; the first mounting plate is provided with a conveying mechanism for conveying shellfish samples, a mincing station for mincing the shellfish samples in the conveying mechanism, a liquid adding and taking station for adding or taking liquid into the conveying mechanism to react with the shellfish samples and a homogenizing station for homogenizing and stirring the shellfish samples in the conveying mechanism; a control module for controlling the whole extraction and detection process is arranged in the control area; a centrifugal station for extracting a liquid to be detected from the mixture of the shellfish samples in the transportation mechanism is arranged in the centrifugal area; a detection station for detecting the type and concentration of toxins in the liquid to be detected extracted by the centrifugal station is arranged in the detection area; the peristaltic pump mounting plate is provided with a first micro peristaltic pump for providing extraction liquid, a second micro peristaltic pump for sucking liquid to be detected, a third micro peristaltic pump for inputting the liquid to be detected of the centrifugal station into the detection station, a fourth micro peristaltic pump for inputting a reaction reagent into the detection station and a fifth micro peristaltic pump for providing cleaning liquid;

the transportation mechanism comprises a chassis steering engine; the chassis steering engine is arranged on the first mounting plate; the chassis steering engine is connected with the bottom plate of the sample pool through a steering wheel and a steering wheel disc of the chassis steering engine; a thin film type pressure-sensitive sensor is arranged in the sample cell chassis; a pretreatment sample pool can be arranged on the film type pressure-sensitive sensor; the bottom of the pretreatment sample pool is provided with a positioning tongue; the sample Chi Depan is provided with a positioning port; the pretreatment sample pool is quickly arranged on the sample pool chassis through the positioning tongue and the positioning port;

the mincing station comprises a first lead screw and a first optical axis; the upper part and the lower part of the first lead screw are respectively connected to the mounting box body through a first lead screw bearing and a second lead screw bearing; the upper part and the lower part of the first optical axis are respectively connected to a left plate of the machine shell through a first optical axis bearing and a second optical axis bearing; the lower part of the first lead screw is connected with a motor shaft of a first stepping motor through a first lead screw coupler; the first stepping motor is arranged on the first mounting plate; a first flange nut is arranged on the first lead screw; the first flange nut is connected with a first connecting beam; the first connecting beam is sleeved on the first optical axis through a first limiting hole; the tail end of the first connecting beam is connected with a first motor support; the first motor support is fixedly connected with a first direct current speed reducing motor and a first baffle cover; the first blocking cover is connected with a first horizontal bearing, and a meat grinding rod through hole is formed in the center of the first blocking cover; a motor shaft of the first direct current speed reducing motor is connected with a mincing coupler, the mincing coupler penetrates through the first horizontal bearing inner ring and the minced meat rod through hole, and two ends of the mincing coupler are respectively connected with the motor shaft of the first direct current speed reducing motor and the minced meat rod for mincing shellfish samples; the first blocking cover is provided with 4 positioning clamping grooves; the transportation mechanism is provided with 4 positioning keys which are matched with the positioning clamping grooves;

the liquid adding and taking station comprises a connecting rod bracket; the bottom of the connecting rod bracket is connected with the first mounting plate; the connecting rod bracket is provided with a steering engine mounting table; the steering engine mounting table is connected with a micro steering engine; the left side and the right side of the connecting rod bracket are fixedly connected with a first fixing rod and a second fixing rod; a first transmission rod and a second transmission rod are connected between the first fixing rod and the second fixing rod through a pin shaft; the tail ends of the first transmission rod and the second transmission rod are connected with a first execution rod and a second execution rod; an extraction liquid dropper, a cleaning liquid dropper and a pipette are connected between the first actuating rod and the second actuating rod; the extract liquid dropper is connected with the outlet of the first micro peristaltic pump through a Teflon pipe; the inlet of the first micro peristaltic pump is connected to the extraction liquid pool through a Teflon pipe; the cleaning liquid dropper is connected with the outlet of the fifth micro peristaltic pump through a Teflon pipe; the inlet of the fifth micro peristaltic pump is connected to the cleaning solution pool through a Teflon pipe; the liquid suction pipe is connected to the inlet of the second micro peristaltic pump through a Teflon pipe; the bottom of the extraction liquid dropper is level with the bottom of the cleaning liquid dropper, and the bottom of the liquid sucker is 3cm lower than the bottom of the extraction liquid dropper; a reinforcing rib plate is arranged between the connecting rod bracket and the steering engine mounting platform;

the homogenizing station comprises a second lead screw and a second optical axis; the upper part and the lower part of the second screw rod are respectively connected to the mounting box body through a third screw rod bearing and a fourth screw rod bearing; the upper part and the lower part of the second optical axis are respectively connected to the mounting box body through a third optical axis bearing and a fourth optical axis bearing; the lower part of the second lead screw is connected with a motor shaft of a second stepping motor through a second lead screw coupler; the second stepping motor is arranged on the first mounting plate; a second flange nut is arranged on the second lead screw and connected with a second connecting beam; the second connecting beam is sleeved on the second optical axis through a second limiting hole; the tail end of the second connecting beam is connected with a second motor support; the second motor support is fixedly connected with a second direct current speed reducing motor; and is connected with a second blocking cover; the second baffle cover is connected with a second horizontal bearing, and a stirring rod through hole is formed in the center of the second baffle cover; a motor shaft of the second direct current speed reducing motor is connected with a stirring coupler; the stirring coupler penetrates through the second horizontal bearing inner ring and the stirring rod through hole; a motor shaft of the second direct current speed reducing motor is connected with a stirring rod for stirring the shellfish sample through a stirring rod coupler; the second retaining cover is provided with 4 positioning clamping grooves; the transportation mechanism is provided with 4 positioning keys which are matched with the positioning clamping grooves; two vibration motor connecting rods are arranged below the second retaining cover; a through hole is formed in the center of the vibration motor connecting rod, and a waterproof vibration motor is connected to the lower portion of the vibration motor connecting rod;

the centrifugal station comprises a centrifugal direct current motor; the centrifugal direct current motor is fixed on the second mounting plate; a motor shaft of the centrifugal direct current motor is connected with a centrifugal mechanism through a centrifugal coupler; the centrifugal station further comprises a centrifugal sample adding screw rod and a centrifugal sampling screw rod; the centrifugal sample adding screw and the centrifugal sampling screw are both positioned above the centrifugal mechanism and are fixedly connected to the mounting box body through a first centrifugal screw fixing frame and a second centrifugal screw fixing frame respectively; a screw rod sliding table on the centrifugal sample adding screw rod is connected with a centrifugal sample adding needle tube; a screw rod sliding table of the centrifugal sampling screw rod is connected with a centrifugal sampling needle tube; the centrifugal sample-adding needle tube is connected to the outlet of the second micro peristaltic pump through a Teflon tube to absorb the liquid to be detected; the centrifugal sampling needle tube is connected to the inlet of the third micro peristaltic pump through a Teflon tube; the centrifugal station further comprises a filtering device; the filtering device comprises a first filtering cover and a second filtering cover; the first filter cover is connected to the outlet of the third micro peristaltic pump through a Teflon pipe; the second filter cover is connected to the detection station through a Teflon pipe so as to input the liquid to be detected into the detection station; a filter screen is arranged between the first filter cover and the second filter cover; the first filter cover is provided with internal threads, the second filter cover is provided with external threads, and the first filter cover and the second filter cover can be screwed and sealed and can fasten the filter screen; the centrifugal mechanism comprises a centrifugal support; a centrifugal test tube can be embedded into the centrifugal support; the centrifugal support is also provided with 4 support screw holes; the centrifugal mechanism further comprises a left buckle cover and a right buckle cover, and the left buckle cover and the right buckle cover are respectively provided with two buckle cover screw holes used for being matched with the support screw holes; the centrifugal test tube is in a shape of , and the test tube opening of the centrifugal test tube is sealed with a layer of elastic sealing film; the centrifugal test tube is symmetrical about the axis of the centrifugal direct current motor, and the tube opening of the centrifugal test tube is kept horizontal.

2. The integrated apparatus for full-automatic toxin extraction and detection according to claim 1,

the detection station comprises a cuvette support; the cuvette support is provided with a cuvette groove, an optical filter groove, a light source optical fiber interface and a spectrometer sensor optical fiber interface; a cuvette is placed in the cuvette groove; the optical filter can be placed in the optical filter groove; the light source optical fiber interface is connected to the LED light source through a light source optical fiber; the optical fiber interface of the spectrometer sensor is connected with the spectrometer sensor through the optical fiber of the spectrometer sensor; the spectrometer sensor is arranged on the control module; the detection station further comprises: a first detection sample adding dropper for dropping a liquid to be detected and a second detection sample adding dropper for dropping a reaction reagent; the dropper ports of the first detection sample adding dropper and the second detection sample adding dropper are positioned above the cuvette; the first detection sample adding dropper is connected to the centrifugal station through a Teflon tube; the second detection sample adding dropper is connected to the outlet of the fourth micro peristaltic pump through a Teflon tube; the inlet of the fourth micro peristaltic pump is connected to the reaction reagent pool through a Teflon tube so as to suck the reaction reagent into the second detection sample adding dropper.

3. The integrated automatic toxin extraction and detection instrument of claim 1,

the mounting box body is provided with a first liquid pool fixing plate, a second liquid pool fixing plate, a third liquid pool fixing plate and a fourth liquid pool fixing plate; the first liquid pool fixing plate is fixedly connected with an extraction liquid pool; the second liquid pool fixing plate is fixedly connected with a reaction reagent pool; the third liquid pool fixing plate is fixedly connected with a cleaning liquid pool; the fourth liquid pool fixing plate is fixedly connected with a waste liquid pool; the extraction liquid pool, the reaction reagent pool and the cleaning liquid pool are also provided with Teflon tube interfaces, and Teflon tubes extend to the bottom of the liquid pool.

4. The integrated apparatus for full-automatic toxin extraction and detection according to claim 1,

the mounting box body is provided with a front cabinet door which is rotatably connected to the mounting box body so as to close the mounting box body; a front door handle and a display screen which is electrically connected with the control module to display operation options and detection results are arranged on the front door of the shell; a first permanent magnet is arranged on the inner side of the front door of the shell; the mounting box body is provided with a second permanent magnet; the front door of the machine shell is attracted and adsorbed to the installation box body through the first permanent magnet and the second permanent magnet so as to close the installation box body.

5. The integrated apparatus for full-automatic toxin extraction and detection according to claim 1,

the top of the installation box body is connected with a top handle and is provided with a switch sliding cover; the switch sliding cover is connected to the top of the installation box body in a sliding mode and fixed through a positioning pin.