CN113288858A - Facial mask containing stem cell exosomes and preparation method thereof - Google Patents
Facial mask containing stem cell exosomes and preparation method thereof Download PDFInfo
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- CN113288858A CN113288858A CN202110728360.3A CN202110728360A CN113288858A CN 113288858 A CN113288858 A CN 113288858A CN 202110728360 A CN202110728360 A CN 202110728360A CN 113288858 A CN113288858 A CN 113288858A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention discloses a facial mask containing stem cell exosomes, which comprises a facial mask body, wherein the facial mask body consists of an essence liquid layer and a silk cloth carrier; wherein the essence layer consists of aloe gel liquid and Chinese medicinal additives, and the mass ratio of the aloe gel to the Chinese medicinal additives is 50-60: 5-10; the preparation process of the traditional Chinese medicine additive comprises the following steps: mixing and crushing orange flower, cucumber, mint and honeysuckle to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with deionized water, stirring at 70-90 deg.C for 30-40min, and filtering to obtain Chinese medicinal additive; the facial mask obtained by the invention has the advantages of whitening, moisturizing, refreshing skin and regulating grease secretion; the stem cell exosome is used as a raw material, so that the mask has good repairing and regenerating functions.
Description
Technical Field
The invention belongs to the technical field of facial masks, and particularly relates to a facial mask containing stem cell exosomes and a manufacturing method thereof.
Background
Exosomes are nanoscale outer lipid bilayer vesicles released outside cells by exocytosis, and can be present in various types of extracellular body fluids such as blood, urine, amniotic fluid, saliva, cerebrospinal fluid and even breast milk, and researches show that the exosomes contains cell-specific proteins, lipids and nucleic acids which can be used as signal molecules to be transmitted to other cells so as to change the functions of other cells, such as: exosomes are involved in the regulation of basic physiological processes such as neuronal communication, antigen presentation, immune response, organ development and reproduction, as well as in the regulation of pathological processes such as cancer progression, cardiovascular disease, inflammation, neurodegenerative disease, viral infection and prion transmission.
The existing facial mask is added with other chemicals, so that the existing facial mask has certain side effect on skin after long-term use, has simpler function, can only play a role in moisturizing usually, and cannot play a good role in repairing damaged skin.
Disclosure of Invention
The invention aims to solve the problems that the existing facial mask is added with other chemicals, so that the existing facial mask has certain side effect on skin after long-term use, has simpler function, can only play a role in moisturizing usually, and does not have good effect on damaged and repaired skin, and provides a facial mask containing stem cell exosomes and a manufacturing method thereof.
The purpose of the invention can be realized by the following technical scheme:
a facial mask containing stem cell exosomes comprises a facial mask body, wherein the facial mask body consists of an essence liquid layer and a silk cloth carrier;
wherein the essence liquid layer consists of aloe gel liquid and Chinese medicinal additives, and the mass ratio of the aloe gel liquid to the Chinese medicinal additives is 50-60: 5-10;
the preparation process of the traditional Chinese medicine additive comprises the following steps: mixing and crushing orange flower, cucumber, mint and honeysuckle to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with deionized water, stirring at 70-90 deg.C for 30-40min, and filtering to obtain Chinese medicinal additive.
Preferably, the mass ratio of the orange flowers to the cucumbers to the mint to the honeysuckle is controlled to be 50-80: 20-50: 10-40: 10-40 parts of; the mass ratio of the traditional Chinese medicine powder to the deionized water is controlled to be 10-20: 20-30.
Preferably, the preparation process of the aloe gel liquid comprises the following steps: adding aloe gel, deionized water, Glycyrrhrizae radix extract and hyaluronic acid, reacting, adding glycerol, glycine, butanediol and isothiazolinone, reacting, adding disodium EDTA and biological enzyme, reacting again, and standing for 30min to obtain aloe gel solution.
Preferably, the mass ratio of the aloe gel, the deionized water, the glycerol, the glycine, the butanediol, the liquorice extracting solution, the isothiazolinone, the hyaluronic acid, the EDTA disodium and the biological enzyme is controlled to be 60-80:5-10:10-18:5-10:5-10:5-10:0.5-2:15-30:0.5-2: 5-10.
A method for preparing a mask containing stem cell exosomes comprises the following steps:
putting the stem cells into a cell culture medium, adding an activating reagent, and carrying out stem cell suspension culture for 12-15 hours to obtain activated adipose-derived stem cells;
taking the activated stem cells out of the culture medium, adding normal saline, cleaning, adding enzyme liquid for digestion treatment, and culturing for three to six generations after the stem cells grow to 80-90% and are fused; centrifuging the culture solution after passage to remove cells and cell debris to obtain supernatant;
mixing the supernatant, D-Hanks buffer solution and cryoprotectant according to the weight ratio of 2-4:2-4:1, and storing at 0 ℃ for 12-15 hours to obtain a mixture; centrifuging the cryopreserved mixture to obtain a stem cell exosome;
mixing the stem cell exosomes and the essence liquid layer according to the mass ratio of 1-2: 20-40, and coating the mixture on a silk cloth carrier through a coating machine to obtain the mask containing the stem cell exosomes.
Compared with the prior art, the invention has the beneficial effects that: the traditional Chinese medicine additive contains orange flowers, cucumbers, mint and honeysuckle, so that the mask has the advantages of whitening, moisturizing, refreshing skin and regulating grease secretion;
the aloe gel liquid prepared by the invention can repair damaged skin, restore the natural elasticity of the skin, enable the skin to be natural and glossy, and achieve the effects of removing black, dark and acne and beautifying the skin, and the aloe gel liquid contains less chemical substances and is a natural skin care product;
the stem cell exosome can accelerate the repair and regeneration of skin, and reduce the formation of skin scars by regulating the differentiation degree of fibroblasts; therefore, the mask taking the stem cell exosomes as the raw materials has good repairing and regenerating functions.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A facial mask containing stem cell exosomes comprises a facial mask body, wherein the facial mask body consists of an essence liquid layer and a silk cloth carrier;
wherein the essence liquid layer consists of aloe gel liquid and traditional Chinese medicine additives, and the mass ratio of the aloe gel liquid to the traditional Chinese medicine additives is 50: 5;
the preparation process of the traditional Chinese medicine additive comprises the following steps: mixing and crushing orange flower, cucumber, mint and honeysuckle to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with deionized water, stirring at 70 deg.C for 30min, and filtering to obtain Chinese medicinal additive.
Controlling the mass ratio of orange flower, cucumber, mint and honeysuckle to be 50: 20: 10: 10; the mass ratio of the traditional Chinese medicine powder to the deionized water is controlled to be 10: 20.
The preparation process of the aloe gel liquid comprises the following steps: adding aloe gel, deionized water, Glycyrrhrizae radix extract and hyaluronic acid, reacting, adding glycerol, glycine, butanediol and isothiazolinone, reacting, adding disodium EDTA and biological enzyme, reacting again, and standing for 30min to obtain aloe gel solution.
Controlling the mass ratio of aloe gel, deionized water, glycerol, glycine, butanediol, liquorice extract, isothiazolinone, hyaluronic acid, EDTA disodium and biological enzyme to be 60:5:10:5:5:5:0.5:15:0.5: 5.
Example 2
A facial mask containing stem cell exosomes comprises a facial mask body, wherein the facial mask body consists of an essence liquid layer and a silk cloth carrier;
wherein the essence liquid layer consists of aloe gel liquid and traditional Chinese medicine additives, and the mass ratio of the aloe gel liquid to the traditional Chinese medicine additives is 55: 8;
the preparation process of the traditional Chinese medicine additive comprises the following steps: mixing and crushing orange flower, cucumber, mint and honeysuckle to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with deionized water, stirring at 80 deg.C for 35min, and filtering to obtain Chinese medicinal additive.
Controlling the mass ratio of the orange flowers to the cucumbers to the mint to the honeysuckle to be 65: 35: 25: 25; the mass ratio of the traditional Chinese medicine powder to the deionized water is controlled to be 15: 25.
The preparation process of the aloe gel liquid comprises the following steps: adding aloe gel, deionized water, Glycyrrhrizae radix extract and hyaluronic acid, reacting, adding glycerol, glycine, butanediol and isothiazolinone, reacting, adding disodium EDTA and biological enzyme, reacting again, and standing for 30min to obtain aloe gel solution.
Controlling the mass ratio of aloe gel to deionized water to glycerol to glycine to butanediol to licorice root extract to isothiazolinone to hyaluronic acid to EDTA disodium to biological enzyme to 70:8:14:8:8:8:1.2:22:1.2: 8.
Example 3
A facial mask containing stem cell exosomes comprises a facial mask body, wherein the facial mask body consists of an essence liquid layer and a silk cloth carrier;
wherein the essence liquid layer consists of aloe gel liquid and a traditional Chinese medicine additive, and the mass ratio of the aloe gel liquid to the traditional Chinese medicine additive is 60: 10;
the preparation process of the traditional Chinese medicine additive comprises the following steps: mixing and crushing orange flower, cucumber, mint and honeysuckle to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with deionized water, stirring at 90 deg.C for 40min, and filtering to obtain Chinese medicinal additive.
Controlling the mass ratio of the orange flowers to the cucumbers to the mint to the honeysuckle to be 80: 50: 40: 40; the mass ratio of the traditional Chinese medicine powder to the deionized water is controlled to be 20: 30.
The preparation process of the aloe gel liquid comprises the following steps: adding aloe gel, deionized water, Glycyrrhrizae radix extract and hyaluronic acid, reacting, adding glycerol, glycine, butanediol and isothiazolinone, reacting, adding disodium EDTA and biological enzyme, reacting again, and standing for 30min to obtain aloe gel solution.
Controlling the mass ratio of aloe gel, deionized water, glycerol, glycine, butanediol, liquorice extract, isothiazolinone, hyaluronic acid, EDTA disodium and biological enzyme to be 80:10:18:10:10:10:2:30:2: 10.
Example 4
A method for preparing a mask containing stem cell exosomes comprises the following steps:
putting the stem cells into a cell culture medium, adding an activating reagent, and culturing the stem cells in a stem cell suspension for 12 hours to obtain activated adipose-derived stem cells;
taking the activated stem cells out of the culture medium, adding normal saline, cleaning, adding enzyme liquid for digestion treatment, and culturing for three to six generations after the stem cells grow to 80% and are fused; centrifuging the culture solution after passage to remove cells and cell debris to obtain supernatant;
mixing the supernatant, the D-Hanks buffer solution and the cryoprotectant according to the weight ratio of 2:2:1, and storing for 12 hours at the temperature of 0 ℃ to obtain a mixture; centrifuging the cryopreserved mixture to obtain a stem cell exosome; the cryoprotectant is glycerol;
mixing the stem cell exosomes and the essence liquid layer according to the mass ratio of 1: 20, mixing, and coating on a silk cloth carrier through a coating machine to obtain the mask containing the stem cell exosomes.
Example 5
A method for preparing a mask containing stem cell exosomes comprises the following steps:
putting the stem cells into a cell culture medium, adding an activating reagent, and culturing the stem cells in a stem cell suspension for 13 hours to obtain activated adipose-derived stem cells;
taking the activated stem cells out of the culture medium, adding physiological saline to clean, then adding enzyme liquid to digest, and transferring to six generations for culture after the stem cells grow to 85% and are fused; centrifuging the culture solution after passage to remove cells and cell debris to obtain supernatant;
mixing the supernatant, the D-Hanks buffer solution and the cryoprotectant according to the weight ratio of 3:3:1, and storing for 14 hours at the temperature of 0 ℃ to obtain a mixture; centrifuging the cryopreserved mixture to obtain a stem cell exosome; the cryoprotectant is glycerol;
mixing the stem cell exosomes and the essence liquid layer according to the mass ratio of 1.5: 30, and coating the mixture on a silk cloth carrier through a coating machine to obtain the mask containing the stem cell exosomes.
Example 6
A method for preparing a mask containing stem cell exosomes comprises the following steps:
putting the stem cells into a cell culture medium, adding an activating reagent, and culturing the stem cell suspension for 15 hours to obtain activated adipose-derived stem cells;
taking the activated stem cells out of the culture medium, adding physiological saline to clean, then adding enzyme liquid to digest, and transferring to six generations for culture after the stem cells grow to 90% and are fused; centrifuging the culture solution after passage to remove cells and cell debris to obtain supernatant;
mixing the supernatant, the D-Hanks buffer solution and the cryoprotectant according to the weight ratio of 4:4:1, and storing for 15 hours at the temperature of 0 ℃ to obtain a mixture; centrifuging the cryopreserved mixture to obtain a stem cell exosome; the cryoprotectant is glycerol;
mixing the stem cell exosomes and the essence liquid layer according to the mass ratio of 2: 40, and coating the mixture on a silk cloth carrier through a coating machine to obtain the mask containing the stem cell exosomes.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (5)
1. A facial mask containing stem cell exosomes, characterized in that: comprises a mask body, wherein the mask body consists of an essence layer and a silk cloth carrier;
wherein the essence liquid layer consists of aloe gel liquid and Chinese medicinal additives, and the mass ratio of the aloe gel liquid to the Chinese medicinal additives is 50-60: 5-10;
the preparation process of the traditional Chinese medicine additive comprises the following steps: mixing and crushing orange flower, cucumber, mint and honeysuckle to obtain traditional Chinese medicine powder; mixing the Chinese medicinal powder with deionized water, stirring at 70-90 deg.C for 30-40min, and filtering to obtain Chinese medicinal additive.
2. The mask containing the stem cell exosomes according to claim 1, wherein the mass ratio of orange flowers, cucumbers, mint and honeysuckle is controlled to be 50-80: 20-50: 10-40: 10-40 parts of; the mass ratio of the traditional Chinese medicine powder to the deionized water is controlled to be 10-20: 20-30.
3. The mask containing the stem cell exosomes according to claim 1, wherein the preparation process of the aloe gel solution comprises the following steps: adding aloe gel, deionized water, Glycyrrhrizae radix extract and hyaluronic acid, reacting, adding glycerol, glycine, butanediol and isothiazolinone, reacting, adding disodium EDTA and biological enzyme, reacting again, and standing for 30min to obtain aloe gel solution.
4. The mask containing the stem cell exosomes according to claim 3, wherein the mass ratio of aloe gel, deionized water, glycerol, glycine, butanediol, licorice extracting solution, isothiazolinone, hyaluronic acid, disodium EDTA and biological enzyme is controlled to be 60-80:5-10:10-18:5-10:5-10:5-10:0.5-2:15-30:0.5-2: 5-10.
5. A method for preparing a mask containing stem cell exosomes is characterized by comprising the following steps:
putting the stem cells into a cell culture medium, adding an activating reagent, and carrying out stem cell suspension culture for 12-15 hours to obtain activated adipose-derived stem cells;
taking the activated stem cells out of the culture medium, adding normal saline, cleaning, adding enzyme liquid for digestion treatment, and culturing for three to six generations after the stem cells grow to 80-90% and are fused; centrifuging the culture solution after passage to remove cells and cell debris to obtain supernatant;
mixing the supernatant, D-Hanks buffer solution and cryoprotectant according to the weight ratio of 2-4:2-4:1, and storing at 0 ℃ for 12-15 hours to obtain a mixture; centrifuging the cryopreserved mixture to obtain a stem cell exosome;
mixing the stem cell exosomes and the essence liquid layer according to the mass ratio of 1-2: 20-40, and coating the mixture on a silk cloth carrier through a coating machine to obtain the mask containing the stem cell exosomes.
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| CN202110728360.3A CN113288858A (en) | 2021-06-29 | 2021-06-29 | Facial mask containing stem cell exosomes and preparation method thereof |
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| CN202110728360.3A CN113288858A (en) | 2021-06-29 | 2021-06-29 | Facial mask containing stem cell exosomes and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114948835A (en) * | 2022-07-16 | 2022-08-30 | 黄喜良 | Production process of traditional Chinese medicine mask |
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2021
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| CN107468616A (en) * | 2017-09-13 | 2017-12-15 | 马书文 | A kind of biology enzyme organic aloe coagulant liquid |
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| CN112842975A (en) * | 2019-11-28 | 2021-05-28 | 洪江市潘多拉中药材生态种植有限公司 | Traditional Chinese medicine silk mask and preparation method thereof |
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