CN113278659A - Recycling method of lactic acid fermentation acid-containing wastewater - Google Patents
Recycling method of lactic acid fermentation acid-containing wastewater Download PDFInfo
- Publication number
- CN113278659A CN113278659A CN202110589297.XA CN202110589297A CN113278659A CN 113278659 A CN113278659 A CN 113278659A CN 202110589297 A CN202110589297 A CN 202110589297A CN 113278659 A CN113278659 A CN 113278659A
- Authority
- CN
- China
- Prior art keywords
- lactic acid
- reverse osmosis
- wastewater
- fermentation
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 134
- 239000004310 lactic acid Substances 0.000 title claims abstract description 66
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 66
- 239000002351 wastewater Substances 0.000 title claims abstract description 47
- 238000000855 fermentation Methods 0.000 title claims abstract description 30
- 230000004151 fermentation Effects 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000004064 recycling Methods 0.000 title claims abstract description 12
- 239000002253 acid Substances 0.000 title claims abstract description 11
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 31
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims abstract description 25
- 239000001527 calcium lactate Substances 0.000 claims abstract description 25
- 229960002401 calcium lactate Drugs 0.000 claims abstract description 25
- 235000011086 calcium lactate Nutrition 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 230000008569 process Effects 0.000 claims abstract description 16
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 13
- 239000012528 membrane Substances 0.000 claims abstract description 11
- 239000000920 calcium hydroxide Substances 0.000 claims abstract description 10
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims abstract description 10
- 229920002261 Corn starch Polymers 0.000 claims abstract description 9
- 239000008120 corn starch Substances 0.000 claims abstract description 9
- 239000002002 slurry Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 6
- 238000001704 evaporation Methods 0.000 claims abstract description 6
- 230000008020 evaporation Effects 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 230000003472 neutralizing effect Effects 0.000 claims abstract description 5
- 239000012466 permeate Substances 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 5
- 230000001502 supplementing effect Effects 0.000 claims abstract description 5
- 241001052560 Thallis Species 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 229920002472 Starch Polymers 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 102000004139 alpha-Amylases Human genes 0.000 claims description 4
- 108090000637 alpha-Amylases Proteins 0.000 claims description 4
- 229940024171 alpha-amylase Drugs 0.000 claims description 4
- 230000001276 controlling effect Effects 0.000 claims description 4
- 238000005086 pumping Methods 0.000 claims description 4
- 238000000526 short-path distillation Methods 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 2
- 239000004382 Amylase Substances 0.000 claims description 2
- 102000013142 Amylases Human genes 0.000 claims description 2
- 108010065511 Amylases Proteins 0.000 claims description 2
- 241000193749 Bacillus coagulans Species 0.000 claims description 2
- 235000019418 amylase Nutrition 0.000 claims description 2
- 229940054340 bacillus coagulans Drugs 0.000 claims description 2
- 229910052740 iodine Inorganic materials 0.000 claims description 2
- 239000011630 iodine Substances 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims description 2
- 210000004080 milk Anatomy 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000011084 recovery Methods 0.000 abstract description 6
- 239000010865 sewage Substances 0.000 abstract description 6
- 230000007613 environmental effect Effects 0.000 abstract description 4
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 description 8
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 229930182843 D-Lactic acid Natural products 0.000 description 2
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 229940022769 d- lactic acid Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/02—Treatment of water, waste water, or sewage by heating
- C02F1/04—Treatment of water, waste water, or sewage by heating by distillation or evaporation
- C02F1/048—Purification of waste water by evaporation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/441—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Water Supply & Treatment (AREA)
- Biotechnology (AREA)
- Hydrology & Water Resources (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Crystallography & Structural Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for recycling acid-containing wastewater generated in lactic acid fermentation, which comprises the following steps: (1) uniformly mixing the corn starch and the reverse osmosis wastewater, and mixing the slurry; (2) dissolving the corn starch which is mixed with the pulp, performing enzymolysis for two times, and filtering by a plate frame to obtain liquefied sugar clear liquid; (3) respectively supplementing reverse osmosis wastewater with liquefied sugar clear liquid and nutrients such as ammonium sulfate and yeast powder to adjust concentration, continuously sterilizing to obtain a microbial culture medium, and performing strain fermentation; (4) after fermentation, solid-liquid separation is carried out to remove thalli, lactic acid is obtained through acidolysis, the concentration of the lactic acid is improved through concentration of an evaporator, and a large amount of lactic acid-containing wastewater is generated in the evaporation process; (5) neutralizing the waste water containing lactic acid with calcium hydroxide to produce calcium lactate, and allowing the calcium lactate solution to permeate through a reverse osmosis membrane to obtain a calcium lactate concentrated solution and reverse osmosis waste water; the generated wastewater returns to the step (1) and the step (3), and the circulation is carried out. The invention relatively saves the sewage treatment cost, improves the recovery rate of the lactic acid, and has important environmental benefit and economic benefit.
Description
Technical Field
The invention relates to the field of lactic acid fermentation processes, in particular to a recycling method of lactic acid fermentation acid-containing wastewater.
Technical Field
With the development of society, especially the progress of science and technology, the rapid development of social productivity is greatly promoted, and the living standard of people is greatly improved; people enjoy the happiness brought by social progress, and can reasonably utilize and pay more attention to the living environment, particularly whether the discharge of the produced and domestic sewage reaches the standard or not.
Lactic acid, an important natural organic acid, is known under the name 2-hydroxypropionic acid and has a relative molecular mass of 90.08. Lactic acid contains one chiral carbon atom in its molecule, so that it has optical activity, and has two optical active configurations, including L-lactic acid and D-lactic acid. Lactic acid is a biological metabolite or metabolic intermediate widely existing in animals, plants and microorganisms, and only L-lactate dehydrogenase capable of metabolizing L-lactic acid exists in human and animal bodies, so that the configuration of lactic acid is very important from the nutritional point of view, and thus L-lactic acid is required to replace D-lactic acid when used in the fields of food and medicine. L-lactic acid is the output raw material of the biodegradable material PLA, and the PLA is the biodegradable material with the most application potential at present.
The microbial fermentation method is a main method for producing lactic acid in the prior art, and a large amount of waste water is generated in the extraction process of the prior art. If the waste water is not properly treated, not only is serious environmental pollution caused, but also a large amount of water resources are wasted, and the production cost of enterprises is increased. Therefore, how to effectively realize energy conservation and emission reduction and reduce the cost is a major subject of healthy development of the lactic acid industry. The wastewater in the lactic acid production process is simply treated and recycled, and has great significance for solving the problem of sewage treatment.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides the method for recycling the waste water generated in the lactic acid production, which saves the process water consumption, saves the sewage treatment cost, improves the recovery rate of the lactic acid and has important environmental and economic benefits compared with the traditional process.
The purpose of the invention is realized as follows:
a method for recycling acid-containing wastewater generated in lactic acid fermentation comprises the following steps:
(1) under the condition that the temperature of corn starch and reverse osmosis wastewater is 40-60 ℃, regulating starch milk to 32% in a slurry regulating tank, regulating the pH of slurry to 5.8-6.0 by using calcium hydroxide, and then adding high-temperature alpha-amylase, wherein the dosage of the amylase is 0.03-0.05%;
(2) carrying out two-step enzymolysis treatment on the mixed solution obtained in the step (1): pumping starch slurry into a jet liquefier by a pump, controlling the temperature at 110-; then carrying out secondary spraying, controlling the temperature at 120-130 ℃, maintaining the temperature in a high-temperature maintaining tank for 3-5 minutes to thoroughly kill the high-temperature-resistant a-starch, further cooling to 60 ℃ under the condition that the iodine test is qualified, simultaneously adjusting the pH value to 4.2, adding saccharifying enzyme, carrying out heat preservation and saccharification for 45-55 hours to reach a saccharification end point, pumping the liquefied sugar solution into a plate frame for filtration to obtain a liquefied sugar clear solution;
(3) adding nutrients such as ammonium sulfate and yeast powder into the liquefied sugar clear liquid obtained in the step (2), supplementing reverse osmosis wastewater, adjusting the concentration, and enabling the obtained mixed liquid total sugar to be 220g/L-250 g/L; sterilizing to obtain a microbial culture medium, and performing strain fermentation;
(4) after fermentation is finished, removing thalli through solid-liquid separation, obtaining lactic acid through acidolysis, concentrating through an evaporator to improve the concentration of the lactic acid, and generating a large amount of lactic acid-containing wastewater in the evaporation process;
(5) and (3) neutralizing the lactic acid-containing wastewater obtained in the step (4) to pH 6.5-7.5 by using a calcium hydroxide solution to produce a calcium lactate solution, and allowing the calcium lactate solution to permeate a reverse osmosis membrane to obtain a calcium lactate concentrated solution and reverse osmosis wastewater.
The strain used for lactic acid fermentation in the step (3) is bacillus coagulans; the total sugar content of the mixed liquid was 235 g/L.
The lactic acid-containing wastewater in the step (4) refers to steam condensate water generated in the processes of an evaporator and short-path distillation of a lactic acid solution.
The temperature of the solution containing calcium lactate and permeating through the reverse osmosis membrane in the step (5) is 40-60 ℃;
and (3) neutralizing the waste water containing the lactic acid in the step (4) by using a calcium hydroxide solution, wherein the pH value is 7.2, producing a calcium lactate solution, and allowing the calcium lactate solution to permeate a reverse osmosis membrane at the temperature of 50 ℃ to obtain a calcium lactate concentrated solution and reverse osmosis waste water.
Has the positive and beneficial effects that: the fermentation temperature of the invention is equivalent to the technical level of the traditional process, the process water consumption is saved, the sewage treatment cost is saved, the recovery rate of lactic acid is improved, and the invention has important environmental benefit and economic benefit.
Drawings
FIG. 1 is a block diagram of the process flow of the present invention.
Detailed Description
The invention is further described with reference to the following drawings and specific embodiments:
examples
As shown in figure 1, 300kg of corn starch and 637.5kg of lactic acid reverse osmosis wastewater are uniformly mixed in a size mixing tank at 50 ℃, calcium hydroxide is added to adjust the pH value to 6.0, and 150g of high temperature resistant alpha-amylase with the enzyme activity unit of 20000U/g is added. Performing secondary spray liquefaction, wherein the primary spray temperature is 97 ℃, keeping the temperature for 60min, the secondary spray temperature is 110 ℃, maintaining the high temperature for 5min, performing vacuum flash evaporation cooling to 95 ℃, cooling to 60 ℃, adding 60g of 1000000U/g saccharifying enzyme, adjusting the pH to 4.8 by using lactic acid, keeping the temperature for 60h, and filtering sugar liquor by using a plate frame after saccharification.
Adding lactic acid reverse osmosis wastewater into the sugar clear liquid, adding 235g/L of mixed liquid total sugar, and adding 0.2% of ammonium sulfate and 0.05% of yeast powder; sterilizing at 115 deg.C for 30min, inoculating lactobacillus, fermenting at 50 deg.C until the lactic acid concentration is 152g/L, fermenting for 36h, and residual sugar is 0.36%.
Adjusting pH of the fermentation broth to 9.0 with calcium hydroxide, heating to 85 deg.C, maintaining the temperature for 6 hr, and filtering with plate-frame filter to remove mycelium; adding 98% sulfuric acid into the filtrate until calcium lactate is completely converted into calcium sulfate, keeping the temperature at 85 ℃ for 6h, performing solid-liquid separation, and removing calcium sulfate crystals.
The purity of the concentration of the lactic acid solution is improved by 10 percent of low-concentration lactic acid through an evaporator and a short-path distillation process, a large amount of steam condensate water is generated, and the steam condensate water is mixed, wherein the content of the lactic acid is 2 percent.
The pH value of the steam condensate water is adjusted to 7.2 by calcium hydroxide, the steam condensate water is filtered and concentrated by a reverse osmosis membrane at the temperature of 50 ℃, the rejection rate of calcium lactate of the reverse osmosis membrane is about 90 percent, and as a result, 83 percent of water in the steam condensate water is removed, the concentration of a calcium lactate concentrate reaches 10.6 percent, the generated reverse osmosis wastewater and the calcium lactate concentrate enter a lactic acid extraction process, and the recovery rate of lactic acid is increased.
300kg of corn starch and 637.5kg of lactic acid reverse osmosis wastewater are recycled, the materials are uniformly mixed in a size mixing tank at 50 ℃, calcium hydroxide is added to adjust the pH value to 6.0, 150g of high temperature resistant alpha-amylase with the enzyme activity unit of 20000U/g is added, secondary injection liquefaction is carried out, wherein the primary injection temperature is 97 ℃, the temperature is kept for 60min, the secondary injection temperature is 110 ℃, the high temperature is kept for 5min, vacuum flash evaporation cooling is carried out to 95 ℃, the temperature is reduced to 60 ℃, 60g of glucoamylase with 1000000U/g is added, the pH value is adjusted to 4.8 by lactic acid, the liquid glucose is filtered by a plate frame after the temperature is kept for 60 h.
Adding lactic acid reverse osmosis wastewater into the sugar clear liquid, adding 235g/L of mixed liquid total sugar, supplementing water shortage by using tap water, adding 0.2% of ammonium sulfate and 0.05% of yeast powder, sterilizing at 115 ℃ for 30min, inoculating lactic acid bacteria for fermentation, wherein the culture temperature is 50 ℃, the lactic acid concentration is 152g/L after fermentation, the fermentation period is 36h, and the residual sugar is 0.36%.
Adjusting pH of the fermentation broth to 9.0 with calcium hydroxide, heating to 85 deg.C, maintaining the temperature for 6h, filtering with plate frame to remove mycelium, adding 98% sulfuric acid into the filtrate until calcium lactate is completely converted into calcium sulfate, maintaining the temperature at 85 deg.C for 6h, separating solid and liquid, and removing calcium sulfate crystal.
The purity of the concentration of the lactic acid solution is improved by 10 percent of low-concentration lactic acid through an evaporator and a short-path distillation process, a large amount of steam condensate water is generated, and the steam condensate water is mixed, wherein the content of the lactic acid is 2 percent.
The pH value of the steam condensate water is adjusted to 7.2 by calcium hydroxide, the steam condensate water is filtered and concentrated by a reverse osmosis membrane at the temperature of 50 ℃, the rejection rate of calcium lactate of the reverse osmosis membrane is about 90 percent, and as a result, 83 percent of water in the steam condensate water is removed, the concentration of a calcium lactate concentrate reaches 10.6 percent, the generated reverse osmosis wastewater and the calcium lactate concentrate enter a lactic acid extraction process, and the recovery rate of lactic acid is increased.
And (3) taking the reverse osmosis wastewater as process water, adding corn starch and sugar clear liquid for recycling, repeating the steps for 9 times, and performing normal lactic acid fermentation.
The fermentation acid production of the invention is equivalent to the technical level of the traditional process, saves the process water consumption, saves the sewage treatment cost, improves the recovery rate of the lactic acid, and has important environmental benefit and economic benefit.
The above are only preferred embodiments of the present invention, and are not intended to limit the present invention, and the present invention is related to the field
The invention is susceptible to various modifications and alternative forms, all as would be apparent to persons skilled in the art. All coming within the spirit and principle of the invention
Any modification, equivalent replacement, improvement and the like made by the method are all included in the protection scope of the invention.
Claims (5)
1. A method for recycling acid-containing wastewater generated in lactic acid fermentation is characterized by comprising the following steps:
(1) uniformly mixing the corn starch and the reverse osmosis wastewater, and mixing the slurry; (2) dissolving the corn starch which is mixed with the pulp, performing enzymolysis for two times, and filtering by a plate frame to obtain liquefied sugar clear liquid; (3) respectively supplementing reverse osmosis wastewater with liquefied sugar clear liquid and nutrients such as ammonium sulfate and yeast powder to adjust concentration, continuously sterilizing to obtain a microbial culture medium, and performing strain fermentation; (4) after fermentation, solid-liquid separation is carried out to remove thalli, lactic acid is obtained through acidolysis, the concentration of the lactic acid is improved through concentration of an evaporator, and a large amount of lactic acid-containing wastewater is generated in the evaporation process; (5) neutralizing the waste water containing lactic acid with calcium hydroxide to produce calcium lactate, and allowing the calcium lactate solution to permeate through a reverse osmosis membrane to obtain a calcium lactate concentrated solution and reverse osmosis waste water; the produced wastewater is returned to the step (1) and the step (3).
2. The recycling method of lactic acid fermentation acid-containing wastewater according to claim 1, characterized by comprising the following steps:
(1) under the condition that the temperature of corn starch and reverse osmosis wastewater is 40-60 ℃, regulating starch milk to 32% in a slurry regulating tank, regulating the pH of slurry to 5.8-6.0 by using calcium hydroxide, and then adding high-temperature alpha-amylase, wherein the dosage of the amylase is 0.03-0.05%;
(2) carrying out two-step enzymolysis treatment on the mixed solution obtained in the step (1): pumping starch slurry into a jet liquefier by a pump, controlling the temperature at 110-; then carrying out secondary spraying, controlling the temperature at 120-130 ℃, maintaining the temperature in a high-temperature maintaining tank for 3-5 minutes to thoroughly kill the high-temperature-resistant a-starch, further cooling to 60 ℃ under the condition that the iodine test is qualified, simultaneously adjusting the pH value to 4.2, adding saccharifying enzyme, carrying out heat preservation and saccharification for 45-55 hours to reach a saccharification end point, pumping the liquefied sugar solution into a plate frame for filtration to obtain a liquefied sugar clear solution;
(3) adding nutrients such as ammonium sulfate and yeast powder into the liquefied sugar clear liquid obtained in the step (2), supplementing reverse osmosis wastewater, adjusting the concentration, and enabling the obtained mixed liquid total sugar to be 220g/L-250 g/L; sterilizing to obtain a microbial culture medium, and performing strain fermentation;
(4) after fermentation is finished, removing thalli through solid-liquid separation, obtaining lactic acid through acidolysis, concentrating through an evaporator to improve the concentration of the lactic acid, and generating a large amount of lactic acid-containing wastewater in the evaporation process;
(5) and (3) neutralizing the lactic acid-containing wastewater obtained in the step (4) to pH 6.5-7.5 by using a calcium hydroxide solution to produce a calcium lactate solution, and allowing the calcium lactate solution to permeate a reverse osmosis membrane to obtain a calcium lactate concentrated solution and reverse osmosis wastewater.
3. The recycling method of lactic acid fermentation acid-containing wastewater according to claim 1 or 2, characterized in that: the strain used in the lactic acid fermentation in the step (3) is bacillus coagulans.
4. The recycling method of lactic acid fermentation acid-containing wastewater according to claim 1 or 2, characterized in that: the lactic acid-containing wastewater in the step (4) refers to steam condensate water generated in the processes of an evaporator and short-path distillation of a lactic acid solution.
5. The recycling method of lactic acid fermentation acid-containing wastewater according to claim 1 or 2, characterized in that: the temperature of the solution containing calcium lactate permeating the reverse osmosis membrane in the step (5) is 40-60 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110589297.XA CN113278659A (en) | 2021-05-28 | 2021-05-28 | Recycling method of lactic acid fermentation acid-containing wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110589297.XA CN113278659A (en) | 2021-05-28 | 2021-05-28 | Recycling method of lactic acid fermentation acid-containing wastewater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113278659A true CN113278659A (en) | 2021-08-20 |
Family
ID=77282143
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110589297.XA Pending CN113278659A (en) | 2021-05-28 | 2021-05-28 | Recycling method of lactic acid fermentation acid-containing wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113278659A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110210001A1 (en) * | 2008-11-10 | 2011-09-01 | Nanjing University Of Technology | Cleaning process of producing lactic acid |
| CN102952831A (en) * | 2011-08-24 | 2013-03-06 | 株式会社日立工业设备技术 | Manufacturing process of purified lactic acid |
| CN104109697A (en) * | 2014-07-08 | 2014-10-22 | 江南大学 | Method for producing citric acid by citric acid wastewater reflux fermentation |
| CN106086093A (en) * | 2016-07-29 | 2016-11-09 | 河南金丹乳酸科技股份有限公司 | The method that lactate fermentation thalline slag preprocess method and circulating fermentation produce lactic acid |
-
2021
- 2021-05-28 CN CN202110589297.XA patent/CN113278659A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110210001A1 (en) * | 2008-11-10 | 2011-09-01 | Nanjing University Of Technology | Cleaning process of producing lactic acid |
| CN102952831A (en) * | 2011-08-24 | 2013-03-06 | 株式会社日立工业设备技术 | Manufacturing process of purified lactic acid |
| CN104109697A (en) * | 2014-07-08 | 2014-10-22 | 江南大学 | Method for producing citric acid by citric acid wastewater reflux fermentation |
| CN106086093A (en) * | 2016-07-29 | 2016-11-09 | 河南金丹乳酸科技股份有限公司 | The method that lactate fermentation thalline slag preprocess method and circulating fermentation produce lactic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103114020B (en) | Method for preparing blueberry fruit wine | |
| CN104774876B (en) | A kind of method of lignocellulose biomass comprehensive utilization | |
| CN101570735B (en) | Method for producing flour yeast by using corn flour as raw material | |
| CN106834368A (en) | A kind of method that utilization lignocellulose for fermentation produces L lactic acid | |
| CN102718325A (en) | Method for culturing high-density oil microalgae to treat yeast industrial wastewater | |
| WO2019223710A1 (en) | Method for producing fuel ethanol using elaeagnus angustifolia as raw material | |
| CN101407761A (en) | Liquid inocula composed of yeast fused strain, Geotrichum candidum Link and Rhizopus, and preparation and use thereof | |
| CN104263793A (en) | Method for treating crystalline dextrose mother liquid | |
| CN102206684A (en) | Fermentation technology for producing calcium lactate with sweet potatoes as raw material | |
| CN104357428A (en) | Liquid submerged fermentation method of xylanase | |
| CN106086093B (en) | Lactic acid fermentation bacteria residue pretreatment method and method for producing lactic acid by circular fermentation | |
| CN106047954B (en) | Method for producing lactic acid and co-producing protein feed through circulating fermentation | |
| CN106244638B (en) | Comprehensive utilization process for producing lactic acid by biomass circulating fermentation | |
| CN103952447A (en) | Method for producing succinic acid by fermentation under anaerobic condition | |
| CN110029134B (en) | Process for producing and extracting glutamic acid | |
| CN104694585A (en) | Production method of erythritol | |
| CN113278659A (en) | Recycling method of lactic acid fermentation acid-containing wastewater | |
| CN102978274A (en) | Method for applying anaerobic dry fermentation technology to treating rice straws to prepare biological flocculating agent | |
| CN111893149A (en) | Method for preparing bacterial cellulose by utilizing soapberry fruit shells | |
| CN106746162A (en) | A kind of processing method of cellulose ethanol production wastewater | |
| CN1074242A (en) | The method that using solid distillers ' grains, yellow water by fermentation are produced lactic acid | |
| CN101348768A (en) | Method for cultivating yeast with beer waste water | |
| CN107904130A (en) | More wheel acetic fermentations and the method for improving mature vinegar quality based on more wheel acetic fermentations | |
| CN113122588A (en) | Method for fermenting lactic acid by mixed carbon source | |
| CN103382491B (en) | Preparation method of citric acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210820 |
|
| RJ01 | Rejection of invention patent application after publication |