CN113264592A - Efficient defluorination microbial flocculant applied to photovoltaic wastewater - Google Patents
Efficient defluorination microbial flocculant applied to photovoltaic wastewater Download PDFInfo
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Abstract
The invention discloses a high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater, which relates to the technical field of biological wastewater treatment materials, and comprises a fluorine-resistant bacterial suspension, nutrient components and a solid-phase carrier; the fluorine-resistant bacterium suspension is a liquid fluorine-resistant bacterium group, and comprises effective fluorine-resistant bacterium viable count of not less than 10 hundred million/g; the nutrient components comprise sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate; the solid phase carrier comprises one or more than two of wheat bran, corn flour and bean pulp. The present invention is prepared from liquid fluorine-resistant flora obtained from sludge contaminated by fluorine-containing organic compounds, sucrose, glycerol, urea, ascorbic acid, dipotassium hydrogen phosphate, and potassium dihydrogen phosphate. The microbial flocculant prepared from the raw materials has selectivity on fluorine ions, is high in efficiency when used for treating fluorine-containing industrial wastewater, is small in usage amount, short in flocculation time in a water body, environment-friendly and free of secondary pollution.
Description
Technical Field
The invention relates to the technical field of biological wastewater treatment materials, and particularly relates to a high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater.
Background
The photovoltaic industry refers to a photovoltaic conversion whole industry chain which provides raw materials, batteries, components and systems and auxiliary products required by industry development around the photovoltaic effect of solar power generation, and comprises high-purity polycrystalline silicon raw material production, solar battery and component production, related production equipment manufacturing and the like. Compared with traditional energy sources such as water power, wind power, nuclear power and the like, the solar power generation is cleaner, safer and more reliable, and the system can generate power independently and run in a grid-connected mode, so that the system has a wide prospect. In recent years, accelerating the development of the photovoltaic industry becomes an important direction of new energy revolution in China.
However, although the solar photovoltaic energy is a clean energy and is very environment-friendly, the production process involves a large number of cutting, grinding, corrosion and other processes, the discharged cleaning wastewater contains a large number of fluorine ions, and the photovoltaic industry has become a main source of pollution of the fluorine-containing wastewater at present. The existing research shows that a series of environmental problems are generated along with the migration and enrichment of fluorine element, and the treatment significance of fluorine-containing wastewater is great.
At present, the method for treating the fluorine-containing wastewater in the photovoltaic industry mainly adopts the traditional precipitation method, and the method of adding lime and soluble calcium salt is adopted to generate CaF from fluoride ions2And removing after precipitation. The method can reduce the fluoride concentration to about 10mg/L generally, but can not meet the standards that the fluoride concentration of the industrial wastewater emission reduction and reuse is lower than 5mg/L and is further higher than the sanitary standard of the domestic drinking water that the fluoride concentration is lower than 1 mg/L. At present, besides the precipitation method, chemical flocculating agents are widely applied to the treatment of fluorine-containing wastewater, but different kinds of flocculationThe flocculant is generally in the problems of overlarge usage amount, high treatment cost, large slag formation amount and difficult treatment, wherein aluminum salt and polypropylene flocculants are also difficult to degrade and have potential carcinogenic risks. Compared with chemical flocculants, the microbial flocculant has the advantages of low treatment cost, good treatment effect, stable reaction process, easiness in operation, no secondary pollution to the environment and obvious advantages in industrial wastewater treatment. However, most of the existing microbial flocculants are applied to the treatment of organic wastewater or heavy metal wastewater, and the application of the microbial flocculants to fluorine-containing wastewater is not mature. Therefore, the microbial flocculant for efficiently removing fluorine is sought, the wide application of the microbial flocculant in the aspect of fluorine-containing wastewater treatment is promoted, and the problem to be solved is needed at present.
Disclosure of Invention
In order to solve the problems and achieve the effects of high-efficiency flocculation, improvement of defluorination efficiency and no secondary pollution, the invention provides a high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater, which fills the blank in the technical field of treating fluorine-containing wastewater by using a microbial flocculant, and the specific scheme is as follows:
a high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater comprises a fluorine-resistant bacterium suspension, nutrient components and a solid-phase carrier; the fluorine-resistant bacterium suspension is a liquid fluorine-resistant bacterium group, and comprises effective fluorine-resistant bacterium viable count of not less than 10 hundred million/g; the nutrient components comprise sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate; the solid phase carrier comprises one or more than two of wheat bran, corn flour and bean pulp.
Preferably, each component in the nutrient composition comprises 2-6 parts of sucrose, 2-6 parts of urea, 1-2 parts of ascorbic acid and 0.2-1 part of dipotassium phosphate by mass.
Preferably, the nutrient composition further comprises glycerin, ethylenediaminetetraacetic acid and monopotassium phosphate.
Preferably, each component in the nutrient composition comprises, by mass, 2-6 parts of sucrose, 2-6 parts of urea, 1-2 parts of ascorbic acid, 0.2-1 part of dipotassium hydrogen phosphate, 2-10 parts of glycerol, 0.2-1 part of ethylenediamine tetraacetic acid and 0.1-1 part of potassium dihydrogen phosphate.
Preferably, in the solid phase carrier, the grain diameter of the wheat bran is 200-300 μm, the grain diameter of the corn meal is 10-50 μm, and the grain diameter of the bean pulp is 200-800 μm.
Preferably, the preparation method of the fluorine-resistant bacteria suspension comprises the following steps:
1) taking aerobic activated sludge generated by treating fluorine-containing wastewater in the photovoltaic industry, standing, and absorbing supernatant to perform primary culture in a selective culture medium to obtain enriched seed bacterial liquid;
2) inoculating the enriched seed bacterial liquid into a new selective culture medium for secondary culture; and centrifuging the culture solution to remove the supernatant to obtain concentrated somatic cells, diluting with sterile water, and uniformly mixing to obtain the fluorine-resistant bacterium suspension.
Preferably, the selection medium of step 1) comprises NaF at a concentration of 50 mg/L.
Preferably, the primary culture in the step 1) is static culture, the culture temperature is 33-37 ℃, and the culture condition is 95% N2，5％CO2The culture time is 3-5 days.
Preferably, the OD600 of the enriched seed bacterial liquid in the step 1) is more than 0.5.
Preferably, the inoculation in step 2) is carried out in an amount of 5% vol.
Preferably, the new selection medium of step 2) comprises NaF at a concentration of 100 mg/L.
Preferably, the re-culturing in the step 2) is carried out at the temperature of 30-35 ℃ under the condition of 95% N2，5％CO2The culture time is 5-7 days.
Preferably, the OD600 of the fluorine-resistant bacterium suspension obtained in the step 2) is more than 0.8, and the number of effective fluorine-resistant bacterium living bacteria is not less than 10 hundred million/g.
The culture conditions were 95% N2，5％CO2The components of the atmosphere of the incubator and the volume percentage thereof are indicated.
Preferably, in the efficient fluorine-removing microbial flocculant applied to photovoltaic wastewater, the mass ratio of the solid phase carrier to the fluorine-resistant bacterial suspension is 1 (80-120).
Preferably, in the efficient fluorine-removing microbial flocculant applied to photovoltaic wastewater, the mass ratio of the nutrient components to the fluorine-resistant bacterial suspension is (5-25): (80-120).
In the raw material formula, the selected liquid fluorine-resistant flora has higher fluorine ion selectivity; sucrose, a carbon source with high monosaccharide content, is easier for the flocculation process of flora; the glycerol can keep playing a buffering role, so that the prepared microbial flocculant has better impact resistance; the urea is used as a nutrient nitrogen source, so that the flocculation rate of flora can be improved; ascorbic acid is deficient in fluorine-containing wastewater and can be used as a growth factor to promote the growth of microbial cells; the ethylene diamine tetraacetic acid has the function of chelating heavy metals, and can prevent flora from being influenced by the heavy metals in the fluorine-containing wastewater; dipotassium phosphate dibasic and potassium phosphate monobasic are used as organic salt compounds, so that the ionic strength of the water body can be adjusted, and the reaction rate is enhanced.
Preferably, the preparation method of the high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater comprises the following steps:
(1) uniformly mixing sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate in the nutrient components with the fluorine-resistant bacteria suspension according to a proportion;
(2) if the nutrient components also comprise other components, adding the other components into the product obtained in the step (1), and uniformly mixing, and if the nutrient components do not comprise the other components, directly performing the step (3);
(3) adding the obtained product into a solid phase carrier under the condition of stirring, uniformly mixing, and standing.
Preferably, the standing in the step (3) is carried out at room temperature for 12-72 h.
Preferably, the concentration of sucrose in the nutrient composition in the fluoride-tolerant bacterial suspension is 2-3% wt.
Advantageous effects
The invention has the beneficial effects that:
the invention provides a high-efficiency defluorination microbial flocculant for photovoltaic wastewater treatment, which is prepared from liquid fluorine-resistant flora obtained from sludge polluted by fluorine-containing organic compounds, sucrose, glycerol, urea, ascorbic acid, dipotassium hydrogen phosphate and potassium dihydrogen phosphate. The microbial flocculant prepared from the raw materials has selectivity on fluorine ions, is high in efficiency when used for treating fluorine-containing industrial wastewater, is small in usage amount, short in flocculation time in a water body, environment-friendly and free of secondary pollution.
The efficient defluorination microbial flocculant for the fluorine-containing wastewater in the photovoltaic industry provided by the invention is simple and convenient to operate and use and low in cost.
Drawings
FIG. 1 is a histogram of the change in fluoride ion concentration, wherein the left side of two sets of data at the same time is set as experiment 1, and the right side is set as experiment 2;
FIG. 2 is a graph showing the change in flocculation rate.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
The following examples and comparative examples are parallel runs, with the same processing steps and parameters, unless otherwise indicated.
The preparation method of the efficient defluorination microbial flocculant applied to photovoltaic wastewater in the following embodiment comprises the following steps:
(1) uniformly mixing sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate in the nutrient components with the fluorine-resistant bacteria suspension according to a proportion;
(2) if the nutrient components also comprise other components, adding the other components into the product obtained in the step (1), and uniformly mixing, and if the nutrient components do not comprise the other components, directly performing the step (3);
(3) adding the obtained product into a solid phase carrier under the condition of stirring, uniformly mixing, and standing.
And (4) standing for 24 hours at room temperature in the step (3).
The concentration of sucrose in the nutrient in the fluoride-tolerant bacteria suspension is 3% wt.
Embodiment 1 a high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater, which comprises a fluorine-resistant bacterial suspension, nutrient components and a solid phase carrier; the fluorine-resistant bacterium suspension is a liquid fluorine-resistant bacterium group, and comprises effective fluorine-resistant bacterium viable count of not less than 10 hundred million/g; the nutrient components comprise sucrose, urea, ascorbic acid, dipotassium hydrogen phosphate, glycerol, ethylene diamine tetraacetic acid and potassium dihydrogen phosphate; the solid phase carrier comprises one or more than two of wheat bran, corn flour and bean pulp.
The nutrient components comprise, by mass, 2 parts of sucrose, 2 parts of urea, 1 part of ascorbic acid, 0.2 part of dipotassium hydrogen phosphate, 2 parts of glycerol, 0.2 part of ethylene diamine tetraacetic acid and 0.1 part of potassium dihydrogen phosphate.
The solid phase carrier is obtained by mixing wheat bran, corn flour and bean pulp in a mass ratio of 1:1:1, wherein the particle size of the wheat bran is 300 mu m, the particle size of the corn flour is 10-50 mu m, and the particle size of the bean pulp is 200 mu m and 800 mu m.
The preparation method of the fluorine-resistant bacterium suspension comprises the following steps:
1) taking aerobic activated sludge generated by treating fluorine-containing wastewater in the photovoltaic industry, standing, and absorbing supernatant to perform primary culture in a selective culture medium to obtain enriched seed bacterial liquid;
2) inoculating the enriched seed bacterial liquid into a new selective culture medium for secondary culture; and centrifuging the culture solution to remove the supernatant to obtain concentrated somatic cells, diluting with sterile water, and uniformly mixing to obtain the fluorine-resistant bacterium suspension.
The selection medium in the step 1) comprises NaF with the concentration of 50 mg/L.
The primary culture of the step 1) is static culture, the culture temperature is 33 ℃, and the culture condition is 95% N2，5％CO2The culture time is 3 d.
The OD600 of the enriched seed bacterial liquid in the step 1) is more than 0.5.
Step 2) the inoculation, wherein the inoculation amount is 5% vol.
Step 2) the new selection medium comprises NaF at a concentration of 100 mg/L.
The second culture in the step 2) is carried out, the culture temperature is 30 ℃, and the culture condition is 95% N2，5％CO2The culture time is 5 days.
The OD600 of the fluorine-resistant bacterium suspension obtained in the step 2) is more than 0.8, and the number of effective fluorine-resistant bacterium living bacteria is not less than 10 hundred million/g.
The culture conditions were 95% N2，5％CO2The components of the atmosphere of the incubator and the volume percentage thereof are indicated.
In the efficient defluorination microbial flocculant applied to photovoltaic wastewater, the mass ratio of the solid phase carrier to the fluorine-resistant bacterial suspension is 1: 80.
The efficient defluorination microbial flocculant applied to photovoltaic wastewater has the mass ratio of the nutrient components to the fluorine-resistant bacterial suspension of 1: 85.
Embodiment 2 a high-efficiency defluorinating microbial flocculant applied to photovoltaic wastewater, which comprises a fluorine-resistant bacterial suspension, nutrient components and a solid-phase carrier; the fluorine-resistant bacterium suspension is a liquid fluorine-resistant bacterium group, and comprises effective fluorine-resistant bacterium viable count of not less than 10 hundred million/g; the nutrient components comprise sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate; the solid phase carrier comprises one or more than two of wheat bran, corn flour and bean pulp.
The nutrient components comprise 6 parts of sucrose, 2 parts of urea, 2 parts of ascorbic acid and 0.5 part of dipotassium hydrogen phosphate by mass.
The solid phase carrier is obtained by mixing wheat bran, corn flour and bean pulp in a mass ratio of 1:1:1, wherein the particle size of the wheat bran is 300 mu m, the particle size of the corn flour is 10-50 mu m, and the particle size of the bean pulp is 200 mu m and 800 mu m.
The preparation method of the fluorine-resistant bacterium suspension comprises the following steps:
1) taking aerobic activated sludge generated by treating fluorine-containing wastewater in the photovoltaic industry, standing, and absorbing supernatant to perform primary culture in a selective culture medium to obtain enriched seed bacterial liquid;
2) inoculating the enriched seed bacterial liquid into a new selective culture medium for secondary culture; and centrifuging the culture solution to remove the supernatant to obtain concentrated somatic cells, diluting with sterile water, and uniformly mixing to obtain the fluorine-resistant bacterium suspension.
The selection medium in the step 1) comprises NaF with the concentration of 50 mg/L.
Step 1) the primary culture is static culture at 35 ℃, and the culture temperature is highThe culture condition is 95% N2，5％CO2The culture time was 4 days.
The OD600 of the enriched seed bacterial liquid in the step 1) is more than 0.5.
Step 2) the inoculation, wherein the inoculation amount is 5% vol.
Step 2) the new selection medium comprises NaF at a concentration of 100 mg/L.
Step 2) culturing again at 33 deg.C under 95% N2，5％CO2The culture time is 6 days.
The OD600 of the fluorine-resistant bacterium suspension obtained in the step 2) is more than 0.8, and the number of effective fluorine-resistant bacterium living bacteria is not less than 10 hundred million/g.
The culture conditions were 95% N2，5％CO2The components of the atmosphere of the incubator and the volume percentage thereof are indicated.
In the efficient defluorination microbial flocculant applied to photovoltaic wastewater, the mass ratio of the solid phase carrier to the fluorine-resistant bacterial suspension is 1: 100.
In the efficient defluorination microbial flocculant applied to photovoltaic wastewater, the mass ratio of the nutrient components to the fluorine-resistant bacterial suspension is 10: 105.
Embodiment 3 a high-efficiency defluorinating microbial flocculant applied to photovoltaic wastewater, comprising a fluorine-resistant bacterial suspension, nutrient components and a solid-phase carrier; the fluorine-resistant bacterium suspension is a liquid fluorine-resistant bacterium group, and comprises effective fluorine-resistant bacterium viable count of not less than 10 hundred million/g; the nutrient components comprise sucrose, urea, ascorbic acid, dipotassium hydrogen phosphate, glycerol, ethylene diamine tetraacetic acid and potassium dihydrogen phosphate; the solid phase carrier comprises one or more than two of wheat bran, corn flour and bean pulp.
The nutrient components comprise 6 parts of sucrose, 6 parts of urea, 2 parts of ascorbic acid, 1 part of dipotassium hydrogen phosphate, 10 parts of glycerol, 1 part of ethylene diamine tetraacetic acid and 1 part of potassium dihydrogen phosphate by mass.
The solid phase carrier is obtained by mixing wheat bran, corn flour and bean pulp in a mass ratio of 1:1:1, wherein the particle size of the wheat bran is 300 mu m, the particle size of the corn flour is 10-50 mu m, and the particle size of the bean pulp is 200 mu m and 800 mu m.
The preparation method of the fluorine-resistant bacterium suspension comprises the following steps:
1) taking aerobic activated sludge generated by treating fluorine-containing wastewater in the photovoltaic industry, standing, and absorbing supernatant to perform primary culture in a selective culture medium to obtain enriched seed bacterial liquid;
2) inoculating the enriched seed bacterial liquid into a new selective culture medium for secondary culture; and centrifuging the culture solution to remove the supernatant to obtain concentrated somatic cells, diluting with sterile water, and uniformly mixing to obtain the fluorine-resistant bacterium suspension.
The selection medium in the step 1) comprises NaF with the concentration of 50 mg/L.
The primary culture of the step 1) is static culture, the culture temperature is 37 ℃, and the culture condition is 95% N2，5％CO2The culture time is 5 days.
The OD600 of the enriched seed bacterial liquid in the step 1) is more than 0.5.
Step 2) the inoculation, wherein the inoculation amount is 5% vol.
Step 2) the new selection medium comprises NaF at a concentration of 100 mg/L.
The second culture in the step 2) is carried out, the culture temperature is 35 ℃, and the culture condition is 95% N2，5％CO2The culture time was 7 days.
The OD600 of the fluorine-resistant bacterium suspension obtained in the step 2) is more than 0.8, and the number of effective fluorine-resistant bacterium living bacteria is not less than 10 hundred million/g.
The culture conditions were 95% N2，5％CO2The components of the atmosphere of the incubator and the volume percentage thereof are indicated.
In the efficient defluorination microbial flocculant applied to photovoltaic wastewater, the mass ratio of the solid phase carrier to the fluorine-resistant bacterial suspension is 120.
In the efficient defluorination microbial flocculant applied to photovoltaic wastewater, the mass ratio of the nutrient components to the fluorine-resistant bacterial suspension is 23: 110.
Effect test:
experimental materials:
the water sample is taken from the wastewater of a certain photovoltaic plant in Jiangsu province (the sampling time is 2020, 7, 20 days), bottling is carried out, and the active sludge in the aerobic section is taken and bottled for recording.
The fluorine ion concentration: 50mg/L
Experiment design:
taking 2 250ml conical flasks, adding a certain amount of sludge and wastewater to be treated, and adjusting the suspended solid concentration value of the mixed solution to be about 3000 mg/L; aerating the bottle to maintain the dissolved oxygen in the bottle at about 3 mg/L;
experimental group 1: beaker No. 1 as experimental group 2ml of flocculant prepared in example 1 was added;
experimental group 2: no. 2 beaker as blank group is not added with flocculating agent, and 2ml of distilled water is added;
the solution was sampled every 6 hours to determine the flocculation rate and fluoride ion concentration. And plotting a flocculation rate change curve and a histogram of the fluoride ion concentration change.
A: absorbance of the supernatant of the experimental beaker at 660 nm;
b: the sludge concentration is 3000mg/L absorbance at 660nm of the solution.
As can be seen from FIG. 1, in 24h of water retention time, the fluoride ion concentration of the experiment group 1 added with the flocculant is reduced from 59mg/L to 8mg/L, and the fluoride ion concentration of the experiment group 2 not added with the flocculant is reduced from 59mg/L to 41 mg/L; the good resistance of fluorine-resistant bacteria in the flocculating agent to fluoride ions is proved.
As can be seen from FIG. 2, in the retention time of 24h of the water body, the flocculation rate of the experimental group 1 added with the flocculant is 30%, and the flocculation rate of the experimental group 2 not added with the flocculant is 9%; the flocculation rate of the experimental group 1 added with the flocculant is 21% higher than that of the experimental group 2 not added with the flocculant.
The effect of the flocculants prepared in examples 2 and 3 was monitored by the same method, and the results were: in the retention time of 24h of water body, the concentration of the fluoride ions in the experimental group of example 2 added with the flocculating agent is reduced from 59mg/L to 11mg/L, and the concentration of the fluoride ions in the experimental group of example 2 added with the flocculating agent is reduced from 59mg/L to 7mg/L, so that the good tolerance of the fluoride-tolerant bacteria in the flocculating agent to the fluoride ions is proved. In the retention time of 24h of water, the flocculation rate of the experimental group of the embodiment 2 added with the flocculating agent is 26 percent, and the flocculation rate of the experimental group of the embodiment 3 added with the flocculating agent is 33 percent; the flocculation rate is higher than that of the experimental group 2 without the flocculant.
Analysis shows that the efficient defluorinating microbial flocculant added with the photovoltaic wastewater has a good effect of removing fluorine ions in wastewater with high fluorine ion concentration and greatly improves the flocculation rate of activated sludge.
While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the embodiments and examples, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (10)
1. The utility model provides a be applied to high-efficient defluorination microbial flocculant of photovoltaic waste water which characterized in that: comprises fluorine-resistant bacteria suspension, nutrient components and a solid phase carrier; the fluorine-resistant bacterium suspension is a liquid fluorine-resistant bacterium group, and comprises effective fluorine-resistant bacterium viable count of not less than 10 hundred million/g; the nutrient components comprise sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate; the solid phase carrier comprises one or more than two of wheat bran, corn flour and bean pulp.
2. The efficient defluorination microbial flocculant applied to photovoltaic wastewater according to claim 1 is characterized in that: the nutrient components comprise, by mass, 2-6 parts of sucrose, 2-6 parts of urea, 1-2 parts of ascorbic acid and 0.2-1 part of dipotassium hydrogen phosphate.
3. The efficient defluorination microbial flocculant applied to photovoltaic wastewater according to claim 1 is characterized in that: the nutrient components also comprise glycerol, ethylene diamine tetraacetic acid and potassium dihydrogen phosphate.
4. The efficient defluorination microbial flocculant applied to photovoltaic wastewater as set forth in claim 3, which is characterized in that: the nutrient components comprise, by mass, 2-6 parts of sucrose, 2-6 parts of urea, 1-2 parts of ascorbic acid, 0.2-1 part of dipotassium hydrogen phosphate, 2-10 parts of glycerol, 0.2-1 part of ethylene diamine tetraacetic acid and 0.1-1 part of monopotassium phosphate.
5. The efficient defluorination microbial flocculant applied to photovoltaic wastewater according to claim 1 is characterized in that: in the solid phase carrier, the grain diameter of wheat bran is 200-300 mu m, the grain diameter of corn flour is 10-50 mu m, and the grain diameter of bean pulp is 200-800 mu m.
6. The efficient defluorination microbial flocculant applied to photovoltaic wastewater according to claim 1 is characterized in that: the preparation method of the fluorine-resistant bacterium suspension comprises the following steps:
1) taking aerobic activated sludge generated by treating fluorine-containing wastewater in the photovoltaic industry, standing, and absorbing supernatant to perform primary culture in a selective culture medium to obtain enriched seed bacterial liquid;
2) inoculating the enriched seed bacterial liquid into a new selective culture medium for secondary culture; and centrifuging the culture solution to remove the supernatant to obtain concentrated somatic cells, diluting with sterile water, and uniformly mixing to obtain the fluorine-resistant bacterium suspension.
7. The efficient defluorination microbial flocculant applied to photovoltaic wastewater as set forth in claim 6, which is characterized in that: step 1), the selection culture medium comprises NaF with the concentration of 50 mg/L; the primary culture of the step 1) is static culture, the culture temperature is 33-37 ℃, and the culture condition is 95% N2，5％CO2The culture time is 3-5 days; the OD600 of the enriched seed bacterial liquid in the step 1) is more than 0.5.
8. The efficient defluorination microbial flocculant applied to photovoltaic wastewater as set forth in claim 6, which is characterized in that: inoculating in the step 2), wherein the inoculation amount is 5% vol; step 2) the new selection medium comprises NaF with the concentration of 100 mg/L; re-culturing at 30-35 deg.C under 95% N2，5％CO2The culture time is 5-7 d; the OD600 of the fluorine-resistant bacterium suspension obtained in the step 2) is more than 0.8, and the number of effective fluorine-resistant bacterium living bacteria is not less than 10 hundred million/g.
9. The efficient defluorination microbial flocculant applied to photovoltaic wastewater according to claim 1 is characterized in that: in the efficient defluorination microbial flocculant applied to photovoltaic wastewater, the mass ratio of the solid phase carrier to the fluorine-resistant bacterium suspension is 1 (80-120); in the efficient fluorine-removing microbial flocculant applied to photovoltaic wastewater, the mass ratio of nutrient components to fluorine-resistant bacteria suspension is (5-25): 80-120.
10. The high-efficiency defluorination microbial flocculant applied to photovoltaic wastewater as set forth in any one of claims 1 to 9, which is characterized in that: the preparation method of the efficient defluorination microbial flocculant applied to photovoltaic wastewater comprises the following steps:
(1) uniformly mixing sucrose, urea, ascorbic acid and dipotassium hydrogen phosphate in the nutrient components with the fluorine-resistant bacteria suspension according to a proportion;
(2) if the nutrient components also comprise other components, adding the other components into the product obtained in the step (1), and uniformly mixing, and if the nutrient components do not comprise the other components, directly performing the step (3);
(3) and under the stirring condition, adding the obtained product into a solid phase carrier, uniformly mixing, and standing to obtain the high-efficiency defluorination microbial flocculant applied to the photovoltaic wastewater.
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Citations (4)
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|---|---|---|---|---|
| CN103274507A (en) * | 2013-05-17 | 2013-09-04 | 中南大学 | Biological flocculant for processing fluorine and calcium in wastewater and preparation and application methods thereof |
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| US20190100452A1 (en) * | 2016-03-09 | 2019-04-04 | Veolia Water Solutions & Technologies Support | Biological removal of micropollutants from wastewater |
| CN112456654A (en) * | 2020-11-10 | 2021-03-09 | 西安建筑科技大学 | Preparation method and application of biological seed crystal and method for synchronously removing calcium and fluorine in underground water |
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