CN109502898B - Resource full utilization method of rice protein production wastewater - Google Patents
Resource full utilization method of rice protein production wastewater Download PDFInfo
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- CN109502898B CN109502898B CN201811463536.1A CN201811463536A CN109502898B CN 109502898 B CN109502898 B CN 109502898B CN 201811463536 A CN201811463536 A CN 201811463536A CN 109502898 B CN109502898 B CN 109502898B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/283—Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/722—Oxidation by peroxides
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/78—Treatment of water, waste water, or sewage by oxidation with ozone
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The invention discloses a resource full utilization method of rice protein production wastewater, which comprises the following steps: (1) separating impurities in the rice protein production wastewater; (2) treating the rice protein wastewater after impurity removal by using geotrichum candidum, saccharomycetes and photosynthetic bacteria, and recovering mycoprotein; (3) and (3) carrying out ozone/hydrogen peroxide/activated carbon oxidation treatment on the wastewater with the recovered mycoprotein to reach the standard, and then recycling. The invention realizes the resource utilization of the rice protein production wastewater, the obtained mycoprotein can be used for animal feed, the economic value is improved, and the wastewater after reaching the standard can be used for production cleaning and land flushing. The invention has simple process, less equipment and low cost, and can carry out resource full utilization on the wastewater.
Description
Technical Field
The invention relates to the field of sewage treatment, in particular to a resource full utilization method of rice protein production wastewater.
Background
The wastewater produced by the rice protein is large in amount, and if the wastewater is directly discharged without being treated, on one hand, a large amount of valuable nutrients such as protein, saccharides and fat contained in the wastewater are lost, so that the waste of resources is caused; on the other hand, the high-concentration organic wastewater is directly discharged into rivers, lakes and seas, which causes frequent occurrence of red tide and water bloom and serious environmental pollution. Therefore, how to effectively and efficiently treat the rice protein wastewater is a troublesome problem which is necessarily faced by enterprises. Meanwhile, how to utilize the wastewater and change the current situation of wastewater treatment which wastes resources and pollutes the environment become the key point of scientific research.
The COD content in each liter of rice protein wastewater reaches 15000-25000mg, the BOD content reaches 5000-10000mg, the concentration is higher, the wastewater belongs to high-concentration wastewater discharged in the rice protein processing process, in addition, the wastewater is acidic, high in conductivity and easy to decay, the BOD and the COD are hundreds of times higher than the national discharge standard, and suspended matters greatly exceed the national discharge standard by 70 mg/L.
At present, the method for treating the rice protein sewage mainly comprises a physical method, a chemical method and a biological activated sludge method, but the physical method can not really remove organic matters in the sewage, the chemical method has poor effect on removing high-concentration organic matters, and can completely remove low-concentration organic matters, but the organic matters are wasted, and the biological activated sludge has high process load, large sludge production amount and difficult treatment. The methods directly discharge the wastewater treatment, and cannot recover the nutrient substances in the wastewater, thereby causing resource waste.
Disclosure of Invention
Aiming at the problems in the prior art, the applicant of the invention provides a resource full utilization method of rice protein production wastewater. The rice protein sewage is treated by geotrichum candidum, saccharomycetes and photosynthetic bacteria with higher mycoprotein content and nutrient content in sequence to recover mycoprotein, and the rice protein sewage can be used as feed; meanwhile, the wastewater is further treated by ozone oxidation, so that organic matters are oxidized into carbon dioxide and water, and the obtained water can be used for flushing, production and cleaning.
The technical scheme of the invention is as follows:
a resource full utilization method of rice protein production wastewater comprises the following steps:
(1) impurity removal: installing a filter screen at the inlet of the wastewater tank to intercept large-particle impurities in the rice protein wastewater;
(2) pretreating geotrichum candidum and yeast: inoculating geotrichum candidum and yeast liquid into the rice protein wastewater after impurity removal, and fermenting for 16-24h at the temperature of 26-30 ℃;
(3) post-treatment of photosynthetic bacteria: inoculating photosynthetic bacteria liquid into the wastewater treated in the step (2), fermenting for 24-48h at the temperature of 26-30 ℃, and performing dynamic culture;
(4) and (3) recovering mycoprotein: carrying out solid-liquid separation on the fermented wastewater, collecting filter residues, drying at constant temperature, crushing and packaging;
(5) oxidation retreatment; carrying out ozone/hydrogen peroxide/activated carbon oxidation on the clear liquid obtained in the step (4) after solid-liquid separation;
(6) filtering and separating: and (4) filtering and separating the wastewater treated in the step (5), and finally producing and recycling the obtained water.
The mesh number of the filter screen in the step (1) is 40-80 meshes.
In the step (2), the addition amount of the geotrichum candidum and the yeast liquid is 10-20% of the weight of the wastewater; the mass ratio of the geotrichum candidum to the yeast is 1: 1-3.
The yeast is a mixture of two or more of candida tropicalis, candida utilis, candida lipolytica and pseudoendospora fibuligera.
In the step (3), the adding amount of the photosynthetic bacteria liquid is 10-20% of the weight of the wastewater.
In the step (3), the photosynthetic bacteria are one or a mixture of purple non-sulfur bacteria and rhodopseudomonas sphaeroides.
The dynamic culture conditions in the step (3) are as follows: the rotating speed in the waste water tank is 100-160 r/min.
The solid-liquid separation condition in the step (4) is as follows: the rotating speed is 5000-; the constant temperature drying temperature is 50-55 deg.C.
The oxidation retreatment method in the step (5) comprises the following steps: ozone is prepared by a water-cooled oxygen source ozone generator, and an ozone mixer and a gas-liquid mixing pump are used for improving the dissolution rate of the ozone in the sewage, wherein the flow rate of the ozone generator is 1-3L/min, the addition amount of hydrogen peroxide is 0.1-0.4% of the total amount of the sewage, the addition amount of activated carbon is 0.2-0.5% of the total amount of the sewage, and the reaction time is 60-120 min.
The beneficial technical effects of the invention are as follows:
1. the rice protein wastewater is pretreated by using geotrichum candidum and yeast (two or more of candida tropicalis, candida utilis, candida lipolytica and paecilomyces capsulatus), macromolecular substances such as polysaccharide and protein are decomposed into monosaccharide, amino acid and low-molecular organic acid, and the rice protein wastewater has strong decomposition and conversion capacity on grease; because the rice protein wastewater contains rich components such as saccharides, proteins, fats and the like, a single bacterium is difficult to fully utilize all nutrient substances, a plurality of strains with different nutritional characteristics are required to be mixed and cultured, and organic matters in the wastewater are converted into recyclable single-cell protein to the greatest extent by utilizing the difference of nutrient substances of substrates decomposed and converted by the strains. The geotrichum candidum can utilize soluble saccharides and ammonium sulfate and urea, has high nutritive value, has the mycoprotein content of more than 45 percent, and has excellent performance; the yeast can grow dominantly in a partial acid environment, has high propagation speed, high content of mycoprotein, no toxicity, no pathogenicity and stable performance, and can utilize organic matters such as macromolecular protein, fat and the like. And the performance difference of the yeasts of the same species is small, so that the geotrichum candidum dominant bacteria and the yeasts are mixed for use, and the nutrient substances are utilized to the maximum extent.
2. The rice protein wastewater is post-treated by using photosynthetic bacteria, wherein the photosynthetic bacteria are purple non-sulfur bacteria and rhodopseudomonas sphaeroides, the somatic cells of the photosynthetic bacteria contain protein, carbohydrate, vitamins and various amino acids, the bacterial sludge is rich in protein and amino acids, can be used as a feed additive and the like, has a certain economic value, the content of the somatic protein reaches about 60 percent, and the cell components of the photosynthetic bacteria are superior to those of yeast and other species. However, the photosynthetic bacteria have a slow utilization rate of macromolecular substances and a fast utilization rate of low-molecular organic acids, amino acids and saccharides. Therefore, the product of geotrichum candidum and the yeast is the nutrient substrate required by the photosynthetic bacteria, and the nutrient substances in the wastewater are fully utilized through the pretreatment and the post-treatment of the three bacteria.
3. Under the dynamic condition, the photosynthetic bacteria can obtain more energy through respiration under the micro-aerobic condition, so that the assimilation capability of the photosynthetic bacteria on small molecular organic matters is improved, and the growth advantages of the photosynthetic bacteria are kept.
4. The invention uses ozone oxidation technology to remove supernatant water of mycoprotein after fermentation. Because the water can not reach the discharge standard by using the bacterial fermentation, the water is further treated by using an ozone oxidation process in the subsequent step. Ozone is a safe and pollution-free efficient oxidant, and the treated water can be recycled. However, ozone alone has poor effect and needs to be combined with other reagents. The invention selects hydrogen peroxide and active carbon as catalysts, the hydrogen peroxide can promote the generation of a large amount of hydroxyl radicals, the active carbon is a reducing substance, the surface of the active carbon is oxidized into groups with oxidizing property in the process of decomposing the hydrogen peroxide and participates in the subsequent oxidation reaction, and the active carbon has the functions of adsorption and decoloration, and in addition, due to the porous structure of the active carbon, the active carbon has the function of immobilizing bacteria, so that the incompletely separated bacteria are settled from the wastewater, thereby increasing the separation effect.
5. The invention uses the mixer-gas-liquid mixing pump to improve the dissolution rate of ozone in sewage. The general method for improving the hydrolysis dissolution rate of ozone comprises aeration, microjet and a gas-liquid mixing pump, wherein the gas-liquid mixing pump has a good effect, the dissolution rate of ozone is 40-70%, and meanwhile, ozone mixer equipment is added in the invention, so that the dissolution rate of ozone is further improved.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
A resource full utilization method of rice protein production wastewater comprises the following steps:
(1) impurity removal: installing a filter screen (40 meshes) at the inlet of the wastewater tank to intercept large-particle impurities in the rice protein wastewater;
(2) pretreating geotrichum candidum and yeast: adding geotrichum candidum and yeast bacteria liquid (the mass ratio of the geotrichum candidum to the yeast is 1:1) which account for 10 percent of the weight of the wastewater into the rice protein wastewater after impurity removal, and fermenting for 24 hours at the temperature of 26 ℃; the yeast is a mixture of candida tropicalis, candida utilis and candida lipolytica in a weight ratio of 1:1: 1;
(3) post-treatment of photosynthetic bacteria: inoculating photosynthetic bacteria liquid (purple non-sulfur bacteria) accounting for 10 percent of the weight of the wastewater into the wastewater treated in the step (2), fermenting for 48 hours at the temperature of 26 ℃, and performing dynamic culture (the rotating speed in the wastewater tank is 160 r/min);
(4) and (3) recovering mycoprotein: carrying out solid-liquid separation on the fermented wastewater for 40min at the rotation speed of 5000r/min, collecting filter residue, drying at a constant temperature of 50 ℃, crushing and packaging;
(5) oxidation retreatment; performing ozone/hydrogen peroxide/activated carbon oxidation on the clear liquid obtained by solid-liquid separation in the step (4), wherein the specific method is to prepare ozone by using a water-cooled oxygen source ozone generator, and simultaneously improve the dissolution rate of the ozone in the sewage by using an ozone mixer and a gas-liquid mixing pump, wherein the flow rate of the ozone generator is 1L/min, the addition amount of the hydrogen peroxide is 0.4 percent of the total amount of the sewage, the addition amount of the activated carbon is 0.2 percent of the total amount of the sewage, and the reaction time is 120 min;
(6) filtering and separating: and (4) filtering and separating the wastewater treated in the step (5), and finally producing and recycling the obtained water.
Example 2
A resource full utilization method of rice protein production wastewater comprises the following steps:
(1) impurity removal: a filter screen (with the mesh number of 60 meshes) is arranged at the inlet of the wastewater tank to intercept large-particle impurities in the rice protein wastewater;
(2) pretreating geotrichum candidum and yeast: adding geotrichum candidum and yeast bacteria liquid (the mass ratio of the geotrichum candidum to the yeast is 1:2) which account for 15 percent of the weight of the wastewater into the rice protein wastewater after impurity removal, and fermenting for 20 hours at the temperature of 28 ℃; the yeast is a mixture of candida tropicalis, candida lipolytica and paecilomyces suberectus in a weight ratio of 1:1: 1;
(3) post-treatment of photosynthetic bacteria: inoculating photosynthetic bacteria liquid (rhodopseudomonas sphaeroides) accounting for 15% of the weight of the wastewater into the wastewater treated in the step (2), fermenting for 36 hours at the temperature of 28 ℃, and performing dynamic culture (the rotating speed in a wastewater tank is 140 r/min);
(4) and (3) recovering mycoprotein: carrying out solid-liquid separation on the fermented wastewater for 30min at the rotating speed of 5500r/min, collecting filter residue, drying at constant temperature of 53 ℃, crushing and packaging;
(5) oxidation retreatment; performing ozone/hydrogen peroxide/activated carbon oxidation on the clear liquid obtained by solid-liquid separation in the step (4), wherein the specific method is to prepare ozone by using a water-cooled oxygen source ozone generator, and simultaneously improve the dissolution rate of the ozone in the sewage by using an ozone mixer and a gas-liquid mixing pump, wherein the flow rate of the ozone generator is 2L/min, the addition amount of the hydrogen peroxide is 0.3 percent of the total amount of the sewage, the addition amount of the activated carbon is 0.4 percent of the total amount of the sewage, and the reaction time is 90 min;
(6) filtering and separating: and (4) filtering and separating the wastewater treated in the step (5), and finally producing and recycling the obtained water.
Example 3
A resource full utilization method of rice protein production wastewater comprises the following steps:
(1) impurity removal: installing a filter screen (with the mesh number of 80 meshes) at the inlet of the wastewater tank, and intercepting large-particle impurities in the rice protein wastewater;
(2) pretreating geotrichum candidum and yeast: adding geotrichum candidum and yeast bacteria liquid (the mass ratio of the geotrichum candidum to the yeast is 1:3) which account for 20 percent of the weight of the wastewater into the rice protein wastewater after impurity removal, and fermenting for 16 hours at the temperature of 30 ℃; the yeast is a mixture of candida tropicalis, candida utilis, candida lipolytica and pseudoendospora capsulata in a weight ratio of 1:1:1: 1;
(3) post-treatment of photosynthetic bacteria: inoculating photosynthetic bacteria liquid (the weight ratio of purple non-sulfur bacteria to spherical rhodopseudomonas is 1:1) accounting for 20% of the weight of the wastewater into the wastewater treated in the step (2), fermenting for 24 hours at the temperature of 30 ℃, and performing dynamic culture (the rotating speed in a wastewater tank is 100 r/min);
(4) and (3) recovering mycoprotein: carrying out solid-liquid separation on the fermented wastewater for 20min at the rotating speed of 6000r/min, collecting filter residue, drying at constant temperature of 55 ℃, crushing and packaging;
(5) oxidation retreatment; performing ozone/hydrogen peroxide/activated carbon oxidation on the clear liquid obtained by solid-liquid separation in the step (4), wherein the specific method is to prepare ozone by using a water-cooled oxygen source ozone generator, and simultaneously improve the dissolution rate of the ozone in the sewage by using an ozone mixer and a gas-liquid mixing pump, wherein the flow rate of the ozone generator is 3L/min, the addition amount of the hydrogen peroxide is 0.1 percent of the total amount of the sewage, the addition amount of the activated carbon is 0.5 percent of the total amount of the sewage, and the reaction time is 60 min;
(6) filtering and separating: and (4) filtering and separating the wastewater treated in the step (5), and finally producing and recycling the obtained water.
Claims (5)
1. A resource full utilization method of rice protein production wastewater is characterized by comprising the following steps:
(1) impurity removal: installing a filter screen at the inlet of the wastewater tank to intercept large-particle impurities in the rice protein wastewater;
(2) pretreating geotrichum candidum and yeast: inoculating geotrichum candidum and yeast liquid into the rice protein wastewater after impurity removal, and fermenting for 16-24h at the temperature of 26-30 ℃;
(3) post-treatment of photosynthetic bacteria: inoculating photosynthetic bacteria liquid into the wastewater treated in the step (2), fermenting for 24-48h at the temperature of 26-30 ℃, and performing dynamic culture;
(4) and (3) recovering mycoprotein: carrying out solid-liquid separation on the fermented wastewater, collecting filter residues, drying at constant temperature, crushing and packaging;
(5) oxidation retreatment; carrying out oxidation of ozone, hydrogen peroxide and activated carbon on the clear liquid obtained in the step (4) after solid-liquid separation;
(6) filtering and separating: filtering and separating the wastewater treated in the step (5), and finally producing and recycling the obtained water;
in the step (2), the addition amount of the geotrichum candidum and the yeast liquid is 10-20% of the weight of the wastewater; the mass ratio of the geotrichum candidum to the yeast is 1: 1-3;
the yeast is a mixture of two or more of candida tropicalis, candida utilis, candida lipolytica and pseudoendospora fibuligera;
in the step (3), the photosynthetic bacteria are one or a mixture of purple non-sulfur bacteria and rhodopseudomonas sphaeroides;
the dynamic culture conditions in the step (3) are as follows: the rotating speed in the waste water tank is 100-160 r/min.
2. The method of claim 1, wherein the mesh size of the screen in step (1) is 40-80 mesh.
3. The method as claimed in claim 1, wherein the amount of the photosynthetic bacteria liquid added in step (3) is 10-20% of the weight of the wastewater.
4. The method according to claim 1, wherein the solid-liquid separation conditions in step (4) are as follows: the rotating speed is 5000-; the constant temperature drying temperature is 50-55 deg.C.
5. The method of claim 1, wherein the oxidative reprocessing in step (5) is by: ozone is prepared by a water-cooled oxygen source ozone generator, and an ozone mixer and a gas-liquid mixing pump are used for improving the dissolution rate of the ozone in the sewage, wherein the flow rate of the ozone generator is 1-3L/min, the addition amount of hydrogen peroxide is 0.1-0.4% of the total amount of the sewage, the addition amount of activated carbon is 0.2-0.5% of the total amount of the sewage, and the reaction time is 60-120 min.
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| JPH08243592A (en) * | 1995-03-09 | 1996-09-24 | Ii M Kenkyu Kiko:Kk | Photooxidation water treating device provided with biological pretreatment and post treatment |
| CN101823810A (en) * | 2010-02-12 | 2010-09-08 | 贵州大学 | Method of reducing pollution and recycling organic matter for waste water of potato starch processing |
| CN102815795A (en) * | 2012-09-12 | 2012-12-12 | 广西民族大学 | Method for processing starch wastewater as well as product and application thereof |
| CN206680267U (en) * | 2017-04-21 | 2017-11-28 | 河南省水产科学研究院 | A kind of biological membrane water purifier of pond culture for carrying strain and culture medium |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08243592A (en) * | 1995-03-09 | 1996-09-24 | Ii M Kenkyu Kiko:Kk | Photooxidation water treating device provided with biological pretreatment and post treatment |
| CN101823810A (en) * | 2010-02-12 | 2010-09-08 | 贵州大学 | Method of reducing pollution and recycling organic matter for waste water of potato starch processing |
| CN102815795A (en) * | 2012-09-12 | 2012-12-12 | 广西民族大学 | Method for processing starch wastewater as well as product and application thereof |
| CN206680267U (en) * | 2017-04-21 | 2017-11-28 | 河南省水产科学研究院 | A kind of biological membrane water purifier of pond culture for carrying strain and culture medium |
Non-Patent Citations (1)
| Title |
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| 光合细菌在我国食品工业废水处理中的应用;温志英等;《陕西科技大学学报》;20060331;第24卷(第3期);146-149 * |
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