CN113252919A

CN113252919A - Multi-sample and multi-reagent pretreatment workstation

Info

Publication number: CN113252919A
Application number: CN202110686683.0A
Authority: CN
Inventors: 王振华
Original assignee: Jiaxing Laibao Instrument Technology Co ltd
Current assignee: Jiaxing Laibao Instrument Technology Co ltd
Priority date: 2021-06-21
Filing date: 2021-06-21
Publication date: 2021-08-13

Abstract

The invention discloses a multi-sample and multi-reagent pretreatment workstation, and relates to the technical field of reagent treatment. Comprises a sample container for containing a plurality of detection samples; the number of the reagent nozzles is a plurality, and each reagent nozzle sprays at least one detection reagent; the spray head clamp is used for clamping the reagent spray head; the detection reagent tank is used for storing a detection reagent and is connected with the reagent spray head through a pipeline, and a liquid transfer pump and an electromagnetic valve are arranged on the pipeline; the double-shaft module comprises an X-shaft module and a Y-shaft module and drives the sample containing tool to move in the horizontal direction; and the Z-axis module drives the spray head clamp and the reagent spray head to move in the vertical direction. The invention realizes the full-automatic pretreatment of multiple samples and multiple reagents.

Description

Multi-sample and multi-reagent pretreatment workstation

Technical Field

The invention relates to the technical field of reagent treatment, in particular to a multi-sample and multi-reagent pretreatment workstation.

Background

The sample detection is to detect samples such as blood, body fluid, secretion, excrement and fallen matters through visual observation, physical, chemical, instrumental or molecular biological methods, thereby providing valuable experimental data.

Before the sample is detected, some indexes cannot be directly obtained, and a reagent is usually added to the sample to obtain the required indexes, which is called the pretreatment of the sample. The existing sample pretreatment generally adopts manual operation of a single sample or a single reagent every time, the operation process is time-consuming and labor-consuming, and the condition of inaccurate sample adding amount during manual operation exists. Therefore, a full-automatic pretreatment system with multiple samples and multiple reagents is urgently needed, and the efficiency is improved.

Disclosure of Invention

In order to solve at least one technical problem mentioned in the background art, an object of the present invention is to provide a multi-sample and multi-reagent preprocessing workstation, which implements fully automatic preprocessing of multi-sample and multi-reagent.

In order to achieve the purpose, the invention provides the following technical scheme:

a multi-sample and multi-reagent pretreatment workstation comprises

The sample container is used for containing a plurality of detection samples;

the number of the reagent nozzles is a plurality, and each reagent nozzle sprays at least one detection reagent;

the spray head clamp is used for clamping the reagent spray head;

the detection reagent tank is used for storing a detection reagent and is connected with the reagent spray head through a pipeline, and a liquid transfer pump and an electromagnetic valve are arranged on the pipeline;

the double-shaft module comprises an X-shaft module and a Y-shaft module and drives the sample containing tool to move in the horizontal direction;

and the Z-axis module drives the spray head clamp and the reagent spray head to move in the vertical direction.

Furthermore, a liquid working chamber is arranged on the back of the Z-axis module, and the detection reagent tank, the liquid transfer pump and the electromagnetic valve are placed in the liquid working chamber.

Further, when the reagent spray head sprays two or more detection reagents, the reagent spray head is also connected with a cleaning liquid tank which is connected with a cleaning liquid storage tank through a pipeline.

Furthermore, be provided with the waste liquid bottle that is used for collecting the waste liquid on the biax module.

Furthermore, the middle part of the reagent spray head is provided with a limiting flange.

Furthermore, the periphery of the nozzle clamp is provided with clamping grooves at intervals for clamping reagent nozzles, and the middle of each clamping groove is provided with a clamping groove for accommodating the limiting flange.

Furthermore, the clamping grooves are obliquely arranged, and the bottoms of the clamping grooves are gathered towards the center.

Furthermore, the spray nozzle fixture is made of elastic materials, and at least one side of the clamping groove is provided with a reserved groove for buffering the deformation of the reagent spray nozzle after being installed in the clamping groove.

Further, the sample container is one or two of a test tube and a pore plate.

Furthermore, the device also comprises a touch display screen which is used for inputting control commands of the liquid moving pump, the electromagnetic valve, the double-shaft module and the Z-shaft module and displaying the working state.

Compared with the prior art, the invention has the beneficial effects that: according to the invention, the sample containing device is driven by the double-shaft module to move in the horizontal direction, different detection samples are sequentially placed below the reagent spray head, then the reagent spray head is driven by the Z-shaft module to move up and down, and the corresponding detection reagents are sprayed into the corresponding detection samples, so that the full-automatic pretreatment work of multiple samples and multiple reagents is realized, the manual intervention is avoided, and the sample adding precision is improved.

Drawings

Fig. 1 is a schematic overall structure diagram of an embodiment of the present invention.

Fig. 2 is a schematic internal structure diagram according to an embodiment of the present invention.

Fig. 3 is a schematic view of a reagent nozzle and a nozzle holder according to an embodiment of the present invention.

In the figure: 11. a test tube; 12. an orifice plate; 2. a reagent spray head; 21. a limiting flange; 3. a spray head fixture; 31. a clamping groove; 32. a card slot; 33. reserving a groove; 51. an X-axis module; 52. a Y-axis module; 53. a Z-axis module; 6. a liquid working chamber; 61. mounting grooves; 62. a detection reagent tank; 63. a liquid transferring pump; 64. an electromagnetic valve; 7. a display screen is touched.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 and 2, the present embodiment provides a multi-sample multi-reagent preprocessing workstation, which includes

The sample container is used for containing a plurality of detection samples. The sample holding means may be one or both of the test tube 11 and the well plate 12 depending on the kind of the test sample. If the test sample is blood, body fluid, or the like, the test tube 11 is used for containing the test sample, and if the test sample is a microorganism, the well plate 12 may be used. The cuvette 11 and the well plate 12 may be used simultaneously.

The reagent spray heads 2 are provided with a plurality of numbers, and each reagent spray head sprays at least one detection reagent.

And the spray head clamp 3 is used for clamping the reagent spray head 2.

Referring to fig. 3, in order to mount the reagent spray head 2, a limiting flange 21 is disposed at the middle of the reagent spray head 2. 3 clamping grooves 31 for clamping the main body part of the reagent spray head 2 are arranged on the periphery of the spray head clamp 3 at intervals, and a clamping groove 32 for accommodating the limiting flange 21 is arranged in the middle of each clamping groove 32. The reagent spray head 2 can be firmly fixed on the spray head clamp 3 through the action of the clamping groove 31 and the clamping groove 32.

When the detection reagent is ejected, in order to facilitate the ejection of the detection reagent into the sample container, the outlets of the reagent ejection head 2 need to be as close as possible. Consequently, the slope of the centre gripping groove 31 of this application sets up, and the bottom is gathered together to the center for 2 installation backs of reagent shower nozzle, the reagent export of bottom draws close each other, realizes the accurate injection of detect reagent, avoids unrestrained outside sample splendid attire utensil.

In order to facilitate clamping, the nozzle clamp 3 is made of an elastic material, the opening of the clamping groove 31 is slightly smaller than the diameter of the main body of the reagent nozzle 2, and the reagent nozzle 2 is clamped through the elasticity of the groove wall of the clamping groove 31. At least one side of the clamping groove 31 is provided with a reserved groove 33 for buffering the deformation of the reagent spray head 2 after being installed in the clamping groove 31.

The biaxial module, including the X-axis module 51 and the Y-axis module 52, drives the cuvette 11 and/or the well plate 12 to move in the horizontal direction. Meanwhile, the X-axis module 51 and the Y-axis module 52 reciprocate together in a short distance, so that the oscillation effect can be realized, and the oscillation and the uniform mixing of the detection sample and the detection reagent in the test tube 11 and/or the pore plate 12 can be realized.

The Z-axis module 53 drives the spray head clamp 3 and the reagent spray head 2 to move in the vertical direction, and when the detection reagent is added, the height of the reagent spray head 2 is reduced, so that the bottom of the reagent spray head 2 extends into the test tube 11 and/or the pore plate 12, and the accurate spraying of the detection reagent is realized; when the test sample is switched by moving the test tube 11 and/or the well plate 12 between the two ejections of the test reagent, the height of the reagent ejection head 2 is raised to avoid interfering with the movement of the test tube 11 and/or the well plate 12.

The detection reagent tank 62 stores detection reagent, and is connected to the reagent nozzle 2 via a pipe (not shown), and the pipe is provided with a liquid transfer pump 63 and an electromagnetic valve 64. It is worth mentioning that when the reagent nozzle 2 sprays two or more detection reagents, the reagent nozzle 2 is further connected with a cleaning liquid tank connected with a pipeline for storing pipeline cleaning liquid, and pipeline cleaning liquid is introduced to clean the pipeline when different detection reagents are switched, so that the detection result is prevented from being influenced. Switching between the detection reagent tank 62 and the cleaning solution tank and the reagent spray head can be achieved by a multi-way valve (i.e., an electromagnetic valve 64).

In order to collect waste liquid, a waste liquid bottle (not shown in the figure) for collecting the waste liquid is arranged on the double-shaft module, and in the cleaning process of the pipeline cleaning liquid, the waste liquid bottle is moved to the position right below the reagent spray head 2 to collect the waste liquid.

And the liquid working chamber 6 is arranged on the back of the Z-axis module. The detection reagent tank 62, the liquid-moving pump 63 and the electromagnetic valve 64 are arranged in the liquid working chamber, and a plurality of mounting grooves 61 are arranged in the liquid working chamber 6 in order to realize the mounting of the detection reagent tank 62 and the cleaning liquid tank.

And the touch display screen 7 is used for inputting control commands of the liquid transferring pump 63, the electromagnetic valve 64, the double-shaft module and the Z-shaft module 53 and displaying the working state.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims

1. A multi-sample and multi-reagent pretreatment workstation is characterized by comprising

The sample container is used for containing a plurality of detection samples;

the spray head clamp is used for clamping the reagent spray head;

2. The multi-sample multi-reagent pretreatment workstation of claim 1, wherein a liquid working chamber is arranged at the back of the Z-axis module, and the detection reagent tank, the liquid transfer pump and the electromagnetic valve are placed in the liquid working chamber.

3. The multi-sample multi-reagent pretreatment workstation of claim 1, wherein when the reagent spray head sprays two or more detection reagents, the reagent spray head is further connected with a cleaning liquid tank connected with a pipeline for storing a pipeline cleaning liquid.

4. The multi-sample multi-reagent pretreatment workstation of claim 1 or 3, wherein a waste liquid bottle for collecting waste liquid is arranged on the dual-axis module.

5. The multi-sample multi-reagent pretreatment workstation of claim 1, wherein a limiting flange is disposed at a middle portion of the reagent spray head.

6. The multi-sample multi-reagent pretreatment workstation according to claim 5, wherein clamping grooves for clamping the reagent spray heads are arranged at intervals on the periphery of the spray head clamp, and clamping grooves for accommodating the limiting flanges are arranged in the middle of the clamping grooves.

7. The multi-sample multi-reagent pretreatment workstation of claim 6, wherein the clamping grooves are obliquely arranged, and the bottoms of the clamping grooves converge towards the center.

8. The multi-sample multi-reagent pretreatment workstation according to claim 6 or 7, wherein the nozzle clamp is made of an elastic material, and at least one side of the clamping groove is provided with a reserved groove for buffering the deformation of the reagent nozzle after the reagent nozzle is installed in the clamping groove.

9. A multi-sample multi-reagent preparation workstation as claimed in claim 1 wherein said sample holding means is one or both of a test tube and a well plate.

10. The multi-sample multi-reagent pretreatment workstation of claim 1, further comprising a touch display screen for inputting control commands of the pipetting pump, the solenoid valve, the dual-axis module and the Z-axis module and displaying the working state.