CN113252562A - Erythrocyte volume detection device for chemiluminescence apparatus - Google Patents
Erythrocyte volume detection device for chemiluminescence apparatus Download PDFInfo
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- CN113252562A CN113252562A CN202110563535.XA CN202110563535A CN113252562A CN 113252562 A CN113252562 A CN 113252562A CN 202110563535 A CN202110563535 A CN 202110563535A CN 113252562 A CN113252562 A CN 113252562A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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Abstract
The invention discloses a hematocrit detection device for a chemiluminescence apparatus, which adopts a vertically arranged conical sample adding hole, wherein a circle of annular groove is arranged outside an opening at the top of the sample adding hole, and after cleaning fluid is filled in the annular groove, the cleaning fluid can uniformly flow into the sample adding hole along 360 degrees, so that the side wall of the conical sample adding hole is completely cleaned, and a sample is prevented from remaining on the side wall of the sample adding hole. Meanwhile, the bosses on the two side surfaces of the metal ring are in interference fit with the corresponding cylindrical counter bores to seal the joints, so that the situation that a sample is remained after the test channel is cleaned is avoided. In addition, this device has realized online loading washing liquid through the online loading unit of washing liquid, introduces the bubble when effectively avoiding changing the raw material barrel of washing liquid for beat the liquid pipeline and get into the bubble and lead to the washing liquid to beat the liquid measure inaccurate, finally influence the problem of testing result accuracy. The accuracy of the hematocrit detection is finally improved through the combination of the three points.
Description
Technical Field
The invention relates to a hematocrit detection device for a chemiluminescence apparatus.
Background
The hematocrit is the volume ratio of red blood cells to whole blood, and is an index indirectly reflecting the number and volume of red blood cells. Combining the red blood cell count and hemoglobin content, the mean red blood cell value can be calculated, which contributes to the morphological classification of anemia.
The hematocrit can be integrated in the chemiluminescence detection, but the existing hematocrit detection unit has certain detection error, the structure of the hematocrit detection unit is provided with a sample adding hole, a test channel and a metal ring arranged on the test channel, and the reason for generating the detection error is found through research that the sample adding hole and the test channel are not completely cleaned after the detection is finished every time due to the structural design defect. Specifically, the application of sample hole of current structure is the slope setting, through the application of sample needle application of sample, later washs application of sample hole inner wall and test channel through application of sample needle injection washing liquid, because the application of sample needle inevitably has the movement track deviation, leads to application of sample hole inner wall to have the sample to remain. Meanwhile, in the existing structure, the annular metal rings are arranged on the test channel at intervals, a non-conductive ring is arranged between the two metal rings, the metal rings and the non-conductive ring are sealed through O-shaped rubber rings, when a rubber ring sealing mode is adopted, the rubber rings need to be sleeved on the non-conductive ring, so that when the rubber ring is compressed, a gap is easily formed in the end face contact of the non-conductive ring and the metal rings, a detection sample is easily remained at the gap, and the detection result is influenced. In addition, because there are a plurality of parts to need to carry out the washing liquid repeatedly in the chemiluminescence appearance working process and wash, if the centre need shut down and trade the former storage bucket of washing liquid and make corresponding washing liquid pipeline in get into the air easily, the pipeline that leads to behind application module gets into the bubble and leads to the washing liquid to beat the liquid measure inaccurate, finally influences the testing result accuracy. The above three defects of the existing structure ultimately affect the accuracy of hematocrit detection.
Disclosure of Invention
The purpose of the invention is as follows: to above-mentioned prior art, provide a hematocrit detection device for chemiluminescence appearance, solve the detection error problem that current structural design defect brought.
The technical scheme is as follows: the erythrocyte hematocrit detection device for the chemiluminescence apparatus comprises a vertical conical sampling hole, wherein the bottom outlet of the conical sampling hole is connected with one end of a horizontally arranged test channel, the test channel is formed by connecting through holes at the axial line positions of a left end block, a metal ring, a non-conductive ring, the metal ring and a right end block which are sequentially connected, bosses are arranged on two side surfaces of the metal ring, cylindrical counter bores are arranged on the contact surfaces of the left end block, the non-conductive ring and the right end block and the metal ring, and each contact surface is in interference fit connection with the cylindrical counter bore through the bosses; the two metal rings are connected to the circuit board through signal probes respectively; anti-interference shielding plates are arranged on the two side faces of the test channel and the signal probe; the other end of the test channel is provided with a first pipeline joint, and the first pipeline joint is connected to the waste liquid barrel through a first pump; the toper adds the sample hole open-top outside and is equipped with the round ring channel, after filling up the cleaning solution in the ring channel, the cleaning solution can be followed toper adds sample hole open-top circumference round and evenly flows in the sample hole.
Further, the side surface of the annular groove is connected with a second pipeline joint through a channel, the second pipeline joint is connected to a cleaning liquid online loading unit through a second pump, and the cleaning liquid online loading unit comprises a cleaning liquid raw material barrel, a cleaning liquid storage tank and an electric control valve; the top of the cleaning solution storage tank is provided with a liquid inlet hole and an exhaust hole, the liquid inlet hole is connected with a cleaning solution raw material barrel through a pipeline, and the exhaust hole is connected with the electric control valve; a liquid outlet hole is formed in the bottom of the cleaning liquid storage tank, and a first liquid level sensor for detecting the liquid level height in the tank is arranged at the upper part of the cleaning liquid storage tank; the signal output end of the first liquid level sensor is connected to a controller, and the controller is connected with the control end of the electric control valve; when the liquid level in the cleaning liquid storage tank is lower than the first liquid level sensor, the electric control valve is controlled to vacuumize the cleaning liquid storage tank to form negative pressure so as to supplement the cleaning liquid from the cleaning liquid raw material barrel.
Furthermore, a second liquid level sensor for detecting the liquid level height in the tank is further arranged at the lower part of the cleaning liquid storage tank, and the signal output end of the second liquid level sensor is connected to the controller.
Furthermore, the online cleaning liquid loading unit further comprises an alarm module, and the controller is connected with the alarm module.
Has the advantages that: the erythrocyte hematocrit detection device for the chemiluminescence instrument adopts the conical sample adding hole which is vertically arranged, the outer side of the opening at the top of the sample adding hole is provided with a circle of annular groove, and after the annular groove is filled with cleaning solution, the cleaning solution can uniformly flow into the sample adding hole along the circle of the opening at the top of the conical sample adding hole, so that the side wall of the conical sample adding hole is completely cleaned, and a sample is prevented from being remained on the side wall of the sample adding hole. In addition, set up O type rubber circle between the becket and the non-conducting ring on the test channel in abandoning traditional structure and seal, adopt the boss of becket side and the sealed of the cylinder counter bore interference fit realization junction that corresponds, still have the remaining condition of sample after avoiding the test channel to wash. In addition, this device has realized online loading washing liquid through the online loading unit of washing liquid, removable washing liquid raw material barrel when detecting promptly, introduces the bubble when effectively avoiding changing the former material barrel of washing liquid, leads to the pipeline of application module at the back to get into the bubble and lead to the washing liquid to beat the liquid measure inaccurate, finally influences the problem of testing result accuracy. Through the combination of the three designs, the detection error of the hematocrit caused by the residual sample is finally solved.
Drawings
FIG. 1 is a first schematic perspective view of the apparatus of the present invention;
FIG. 2 is a schematic perspective view of the apparatus of the present invention, excluding the tapered well structure;
FIG. 3 is a third schematic perspective view of the inventive apparatus, without a side panel;
FIG. 4 is a schematic structural diagram of a cleaning solution online loading unit;
FIG. 5 is a schematic view of a cleaning solution reservoir.
Detailed Description
The invention is further explained below with reference to the drawings.
As shown in FIGS. 1 to 3, a hematocrit test device for a chemiluminescence apparatus includes a vertical tapered well 1 and a horizontally disposed test channel 2.
The test channel 2 is formed by connecting through holes at the axial line positions of a left end block, a metal ring 3, a non-conductive ring, the metal ring 3 and a right end block which are sequentially connected, bosses are arranged on two side surfaces of the metal ring 3, cylindrical counter bores are arranged on the contact surfaces of the left end block, the non-conductive ring and the right end block and the metal ring 3, and the contact surfaces are connected with the cylindrical counter bores in an interference fit mode through the bosses to achieve effective sealing of the connection positions. The two metal rings are connected to a circuit board 5 through signal probes 4, respectively. And anti-interference shielding plates 8 are arranged on two side surfaces of the test channel 2 and the signal probe 4.
The bottom exit linkage level of toper sample application hole 1 sets up the one end of test passageway 2, and the other end of test passageway 2 is equipped with first pipeline joint 6, and first pipeline joint 6 is connected to the waste liquid bucket through first pump. A circle of annular groove 7 is arranged on the outer side of the top opening of the conical sampling hole 1, the side surface of the annular groove 6 is connected with a second pipeline joint 9 through a channel, and the second pipeline joint 9 is connected to a cleaning liquid online loading unit through a second peristaltic pump; the second peristaltic pump is used for injecting the cleaning liquid into the annular groove 7, and after the cleaning liquid is filled, the cleaning liquid can uniformly flow into the sample adding hole along a circle of the circumference of the top opening of the conical sample adding hole 1.
When the unit works, a sample is injected into the conical sample adding hole 1 by the sample adding needle, the first peristaltic pump works, the sample is pumped to the position of the metal ring 3 in the test channel 2 to carry out packed cell detection, and a signal on the metal ring 3 is transmitted to the circuit board 5 through the signal probe 4 to be processed; after the detection is finished, the cleaning liquid is injected into the annular groove 7 through the second peristaltic pump, after the cleaning liquid is filled, the cleaning liquid can uniformly flow into the sample adding hole along the circle of the top opening circumference of the conical sample adding hole 1, the cleaning liquid is matched with the first peristaltic pump to work, the conical sample adding hole 1 and the test channel 2 are effectively cleaned, and the cleaning liquid is timely and thoroughly pumped away by the first peristaltic pump and is discharged into the waste liquid barrel.
As shown in fig. 4 and 5, the online cleaning solution loading unit includes a cleaning solution raw material tank 10, a cleaning solution storage tank 11, and an electric control valve 12. The top of the cleaning liquid storage tank 11 is provided with a liquid inlet hole 13 and an exhaust hole 14, the bottom of the cleaning liquid storage tank 11 is provided with a liquid outlet hole 15, the liquid inlet hole 13 is connected with the cleaning liquid raw material barrel 10 through a pipeline, and the exhaust hole 14 is connected with the electric control valve 12. A transparent liquid level detection column is vertically arranged on the side surface of the cleaning liquid storage tank 11, a first liquid level sensor 16 and a second liquid level sensor 18 for detecting the liquid level height in the tank are fixed on the outer side of the transparent liquid level detection column, and other parts of the cleaning liquid storage tank 11 are of opaque structures. Wherein the first liquid level sensor 16 is located at an upper position of the cleaning liquid reservoir 11, and the second liquid level sensor 18 is located at a lower position of the cleaning liquid reservoir 11. The signal output ends of the first liquid level sensor 16 and the second liquid level sensor 18 are connected to a controller 17, and the controller 17 is connected with the control end of the electric control valve 12 and an alarm module 19.
When the liquid level in the cleaning liquid storage tank 11 is lower than the first liquid level sensor 16, the electronic control valve 12 is controlled to vacuumize the cleaning liquid storage tank 11 to form negative pressure so as to replenish the cleaning liquid from the cleaning liquid raw material barrel 10. When the liquid level in the cleaning liquid storage tank 11 is lower than the second liquid level sensor 18 at the lower position, the control alarm module 19 gives an alarm to remind a user to replace the cleaning liquid raw material barrel 10 and stop the additional detection operation of the user until the detection item can be continuously issued after the signal of the second liquid level sensor 18 returns to normal, so that the cleaning liquid pipeline is prevented from entering bubbles to finally influence the detection result.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. The erythrocyte hematocrit detection device for the chemiluminescence apparatus is characterized by comprising a vertical conical sampling hole (1), wherein the bottom outlet of the conical sampling hole (1) is connected with one end of a horizontally arranged test channel (2), the test channel (2) is formed by connecting through holes at the axial line positions of a left end block, a metal ring, a non-conductive ring, the metal ring and a right end block which are sequentially connected, bosses are arranged on two side surfaces of the metal ring, cylindrical counter bores are arranged on the contact surfaces of the left end block, the non-conductive ring and the right end block and the metal ring, and each contact surface is in interference fit connection with the cylindrical counter bore through the bosses; the two metal rings are respectively connected to a circuit board (5) through signal probes (4); anti-interference shielding plates (8) are arranged on two side surfaces of the test channel (2) and the signal probe (4); the other end of the test channel (2) is provided with a first pipeline joint (6), and the first pipeline joint (6) is connected to a waste liquid barrel through a first pump; toper application of sample hole (1) open-top outside is equipped with round ring channel (7), fill in annular channel (7) after the washing liquid, the washing liquid can be followed toper application of sample hole (1) open-top circumference round evenly flows in the application of sample hole.
2. The hematocrit detection unit for the chemiluminescence apparatus according to claim 1, wherein the side of the annular groove (6) is connected to a second pipeline connector (9) through a channel, the second pipeline connector (9) is connected to a cleaning solution online loading unit through a second pump, and the cleaning solution online loading unit comprises a cleaning solution raw material barrel (10), a cleaning solution storage tank (11), and an electrically controlled valve (12); a liquid inlet hole (13) and an exhaust hole (14) are formed in the top of the cleaning liquid storage tank (11), the liquid inlet hole (13) is connected with a cleaning liquid raw material barrel (10) through a pipeline, and the exhaust hole (14) is connected with the electric control valve (12); a liquid outlet hole (15) is formed in the bottom of the cleaning liquid storage tank (11), and a first liquid level sensor (16) for detecting the liquid level height in the tank is arranged at the upper part of the cleaning liquid storage tank (11); the signal output end of the first liquid level sensor (16) is connected to a controller (17), and the controller (17) is connected with the control end of the electric control valve (12); when the liquid level in the cleaning liquid storage tank (11) is lower than the first liquid level sensor (16), the electric control valve (12) is controlled to vacuumize the cleaning liquid storage tank (11) to form negative pressure so as to replenish the cleaning liquid from the cleaning liquid raw material barrel (10).
3. The hematocrit detection unit of claim 2, wherein a second level sensor (18) for detecting a level of the liquid in the tank is further provided at a lower portion of the cleaning liquid reservoir (11), and a signal output terminal of the second level sensor (18) is connected to the controller (17).
4. The hematocrit detection unit for a chemiluminescent instrument according to claim 3 wherein the online loading unit of the cleaning solution further comprises an alarm module (19), the controller (17) being connected to the alarm module (19).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110563535.XA CN113252562B (en) | 2021-05-24 | 2021-05-24 | Erythrocyte volume detection device for chemiluminescence apparatus |
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| CN202110563535.XA CN113252562B (en) | 2021-05-24 | 2021-05-24 | Erythrocyte volume detection device for chemiluminescence apparatus |
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| CN113252562A true CN113252562A (en) | 2021-08-13 |
| CN113252562B CN113252562B (en) | 2022-10-21 |
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Cited By (1)
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| CN113252562B (en) | 2022-10-21 |
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