CN113243485A

CN113243485A - Edible fungus preparation and staple food grain prebiotics for treating diabetes and improving immunity and production technology thereof

Info

Publication number: CN113243485A
Application number: CN202110669422.8A
Authority: CN
Inventors: 王昱蓉; 季静; 王罡
Original assignee: Tianjin Tianda Tianfu Biotechnology Co ltd
Current assignee: Tianjin Tianda Tianfu Biotechnology Co ltd
Priority date: 2021-06-17
Filing date: 2021-06-17
Publication date: 2021-08-13

Abstract

The technology of the invention comprises a formula of a staple food prebiotics culture medium or main material, auxiliary materials and seasonings of Tianfu TNB, edible fungi and fermentation equipment. Staple food grains comprise rice, corn and soybean and selection and breeding methods thereof. The edible fungus is a family-borne edible microorganism flora (FEMF) and an improved auxiliary fungus thereof, the flora structure, the strain of the improved FEMF flora, the structure and the proportion of the auxiliary fungus are clarified, and the microbial inoculum presents sour and sweet taste after fermentation. Fermentation techniques range from 1 to 5 grades, and product techniques include liquid and solid. In order to facilitate fermentation, simple fermentation equipment rotating 360 degrees is designed and manufactured. The blood sugar of young people is stable and normal after eating the product for 17 days; the patients with diabetes of 83 years old are frequently dizzy and have heart pain, and the basic recovery immunity after 3 months is improved; the long-term familial heredity long-term type II diabetes mellitus patients take insulin and Western medicines with the fasting state of 14.2, and are assisted to treat the normal blood sugar in 28 days, and the gastritis and the mystery system diseases are improved.

Description

Edible fungus preparation and staple food grain prebiotics for treating diabetes and improving immunity and production technology thereof

Technical Field

The invention relates to a functional microbial inoculum for treating metabolic syndromes such as diabetes and the like, gastrointestinal chronic diseases and discomfort (capable of dispelling the effects of alcohol) and improving the immunity, and a nutritional staple food grain prebiotic containing plant secondary metabolites and a production technology thereof, belongs to food therapy functional food formed by applying biotechnology, can treat diabetes, improve the blood sugar condition of a patient and improve the immunity, and is convenient to eat, safe and reliable.

Background

At present, metabolic syndromes such as diabetes and the like become increasingly serious diseases affecting public health problems in the world, the metabolic syndromes comprise diabetes, hyperglycemia, hyperuricemia and hyperlipidemia, but the actual blood sugar reduction is difficult to realize by using traditional Chinese medicines. The traditional clinical treatment of diabetes mainly takes oral hypoglycemic drugs, insulin injection and life style intervention (diet and exercise) as main parts, from the scientific research perspective, the clinical detection and analysis of modern diseases are limited to monogenic hereditary diseases or chromosomal diseases, such as type II diabetes, which belongs to complex diseases, and are related to heredity, environment, diet and other aspects, so that no conventional molecular detection and analysis method exists temporarily, and the research and analysis from the aspects of intestinal flora, metabolism, small molecules and the like tend to be performed. In recent years, a novel intervention mode taking intestinal microorganisms as targets develops the treatment of diabetes. Years of research have shown that microorganisms are not effective in all situations and that fermentation with suitable target substrates is only significantly effective. The technology is switched in from the aspects of staple food rice, intestinal flora, metabolism, micromolecules and the like in a food therapy angle, the intervention of safe and non-side-effect characteristic nutrition rice, corn, soybean, microorganisms and the like is used for reducing blood sugar and preventing type II diabetes, the confusion that the diabetes patients eat less or cannot eat rice is broken through, and the diabetes is treated by the rice and microbial food therapy, so that the rice and microbial food therapy brings good news to about 4.25 hundred million diabetes patients worldwide and 1.14 million diabetes patients in China (latest data in 2020).

Meanwhile, through years of research, the combination of the edible fungi, the staple food grains and other prebiotics in the technology can improve the health state, prevent diseases, obviously improve the immunity and the gastrointestinal tract function, and has the functions of regulating the intestinal flora and relieving functional gastrointestinal tract diseases on enteritis.

These functions are also highly relevant to the nutritional quality of the secondary metabolites obtained by the rice and corn.

Disclosure of Invention

1. The technology comprises main materials, auxiliary materials and seasonings of a fermentation culture medium, edible fungi and fermentation equipment, wherein the edible fungi are domestic edible microorganism flora (FEMF) and improved auxiliary bacteria thereof, and the fermentation technology and the fermentation product preparation technology of the main grain prebiotics culture medium are also called Tianfu TNB culture medium.

2. Selection of staple food grains in a staple food grain prebiotics culture medium and a breeding method thereof. The staple food grains can be selected from conventional rice, corn and soybean, but the effect of selecting the rice and the corn with nutritional quality is better. Compared with the common staple food, the nutritional staple food has better effect, and the rice has more benefits such as special nutritional quality rice Tianfu 106, Tianfu 206, Tianfu 409 and Tianfu 307; the corn is nutritional quality corn, such as Tianfu red corn. The main grain with the nutritional quality has better effects on metabolic syndromes such as diabetes and the like and gastrointestinal disorder including rapid hangover alleviation and immunity and disease resistance than common main grains after fermentation of domestic edible microbial flora (FEMF).

The breeding method of the nutritional staple food grains comprises the following steps: the breeding method of the special nutritional quality rice Tianfu 106, Tianfu 206, Tianfu 409 and Tianfu 307 comprises the following steps: the method comprises the steps of firstly screening a large number of common rice containing carotenoid, anthocyanin and the like, screening more than 1000 kinds of domestic and foreign conventional rice varieties aiming at the carotenoid, obtaining the tianfuqing 2 containing the carotenoid through breeding, then hybridizing with local rice, and obtaining the rice with the carotenoid and anthocyanin and high nutrition quality through hybridization progeny nutrition quality breeding. Cold resistance, drought resistance, salt and alkali resistance, strong stress resistance, good taste, large rice grains and japonica rice varieties. The specific characteristics of Tianfu 106, Tianfu 206, Tianfu 409 and Tianfu 307 are that the plant heights are respectively 101cm, 116cm, 117.25cm and 118cm on average; the spike shape is compact, and the spike length respectively reaches 21.00cm, 22.00cm, 23.5cm and 27.00cm on average; the thousand seed weights are respectively 26.14g, 29.91g, 23.51g and 25.00g on average; the standard yield is respectively 607.33 kg/mu, 750.80 kg/mu, 605.20 kg/mu and 558.6 kg/mu on average. The total growth period was 172 days, 170 days, 168 days and 169 days, respectively. The average protein content was 62.46mg/g, 70.78mg/g, 63.93mg/g and 61.79mg/g, respectively. Wherein Tianfu 106, Tianfu 206 and Tianfu 409 are the attributes of the conventional golden rice, contain carotenoid, and the content of the carotenoid is respectively 2.01 mu g/g, 1.36 mu g/g and 3.72 mu g/g on average. Tianfu 307 is a black rice type, contains anthocyanin, has 307 anthocyanin content of 170.36mg/100g, and is mostly cyanidin-3-O-glucoside.

The nutrient corn is prepared by the conventional method: screening corn grains with orange and red seed coats, gathering genes through conventional breeding, selfing the corn with one silky red color year by year to obtain more improved Tianfu orange red grain corn with orange red seed coats, wherein the improved Tianfu orange red grain corn contains carotenoid which is not detected yet, such as lutein, beta-carotene and the like besides zeaxanthin, and the improved Tianfu orange red grain corn with the orange red seed coats which is conventionally bred through HPLC detection contains carotenoid which is not detected yet, such as lutein, beta-carotene and the like besides zeaxanthin.

The soybean is not high in requirement, and instant soybean powder can be used, and black soybean powder and soybean powder can be used.

3. Domestic edible microorganism flora (FEMF) for treating metabolic syndrome such as diabetes and improving immunity

The family-handed edible microbial flora (FEMF) is a relatively complex flora, is cultured and eaten by 90-year-old people in home 50 years ago, and comprises the following components by metagenomic analysis: bacillus, various lactic acid bacteria, bifidobacterium and the like. The microbial inoculum is fermented to show sour and sweet taste.

4. Improved FEMF flora and auxiliary flora structure

1) Metagenome analysis shows that the main effective bacteria of the family-borne edible microorganism flora (FEMF) comprise: bacillus, various lactic acid bacteria, bifidobacterium and the like.

2) In addition to the main effect bacteria, auxiliary bacteria for improvement:

(1) bacillus natto (Bacillus natto): TRIIM 1.01025 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(2) Bacillus licheniformis (Bacillus licheniformis): TRIIM 1.00400 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(3) Bacillus amyloliquefaciens (Bacillus amyloliquefaciens): TRIIM 1.00303 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(4) Bacillus subtilis (Bacillus subtilis): TRIIM 1.01001 originated from the Industrial microbiology institute of Tianjin (TRIIM), and the strain was publicly available from the Industrial microbiology institute of Tianjin (TRIIM).

(5) Streptococcus thermophilus (Streptococcus thermophilus) CICC 6038 is from China center for Industrial culture Collection of microorganisms (CICC) and can be obtained by the public from the China center for culture Collection of Industrial microorganisms.

(6) Lactobacillus plantarum CICC 21810 originates from the China center for Industrial microbial cultures Collection (CICC) and is available to the public from the China center for Industrial microbial cultures Collection.

(7) Lactobacillus rhamnosus (Lactobacillus rhamnosus) CICC 20257 is originated from the China center for Industrial microbial cultures Collection (CICC) and can be obtained by the public from the China center for Industrial microbial cultures Collection.

(8) Lactobacillus casei (Lactobacillus casei) CICC 23185 is from the China center for Industrial microbial culture Collection (CICC) and is available to the public from the China center for Industrial microbial culture Collection.

(9) Lactobacillus paracasei (Lactobacillus casei) CICC 20252 is from the China center for Industrial microbial culture Collection (CICC) and is available to the public from the China center for Industrial microbial culture Collection.

(10) Lactobacillus acidophilus (Lactobacillus acidophilus) CICC 20244 is originated from the China center for the culture Collection of Industrial microorganisms (CICC), and the strain is available to the public from the China center for the culture Collection of Industrial microorganisms.

5. FEMF flora and auxiliary bacteria ratio

The main effective bacteria and auxiliary bacteria in the ratio of 1: 0-1: 1

Wherein, the family-borne main effective bacteria can be used for drawing up a long-term balance flora, and the proportion of the long-term balance flora to the auxiliary flora is 50-100%.

The addition proportion of various auxiliary improving bacteria is 0-20% according to the condition, and the total proportion is not more than 50%.

Setting the use proportion:

1) the main effective edible microbial flora (FEMF) comprises: bacillus, various lactic acid bacteria and bifidobacteria. The use ratio is set to 50% to 100%.

2) Auxiliary improving bacteria

(1) The using proportion of the bacillus natto is set to be 0-20%.

(2) The use ratio of the bacillus licheniformis is set to be 0-20%.

(3) The use ratio of the bacillus amyloliquefaciens is set to be 0% to 20%.

(4) The use ratio of the bacillus subtilis is set to be 0-20%.

(5) Streptococcus thermophilus 0% to 20%.

(6) Lactobacillus plantarum 0-20%.

(7) 0 to 20 percent of lactobacillus rhamnosus.

(8) Lactobacillus casei 0-20%.

(9) Lactobacillus paracasei 0% to 20%.

(10) Lactobacillus acidophilus 0% to 20%.

3) Experiments show that the optimal use ratio is as follows:

(1) the domestic edible microorganism flora (FEMF) major bacteria comprise: bacillus, various lactic acid bacteria and bifidobacteria. The use ratio was set to 80%.

Auxiliary improving bacteria

(2) The using proportion of the bacillus natto is set to be 2%.

(3) The use ratio of the bacillus licheniformis is set to be 2%.

(4) The use ratio of the bacillus amyloliquefaciens is set to be 2%.

(5) The use ratio of the bacillus subtilis is set to be 2%.

(6) And 2% of streptococcus thermophilus.

(7) 2% of lactobacillus plantarum.

(8) And 2% of lactobacillus rhamnosus.

(9) And 2% of lactobacillus casei.

(10) And 2% of lactobacillus paracasei.

(11) 2% of lactobacillus acidophilus.

6. Formula of staple food grain prebiotic fermentation culture medium also called Tianfu TNB culture medium

The main food grain prebiotics fermentation culture medium of the domestic edible microbial flora (FEMF) is also called a main food grain, auxiliary materials and seasoning formula in a Tianfu TNB culture medium:

the auxiliary materials are important and some must be added, and the formula of the auxiliary materials is as follows: milk powder (or milk), soluble collagen, soluble elastin, hydrolyzed collagen peptide, ginseng extract, gingerol (in a small amount), hydrolyzed corn protein extract, soybean polypeptide, Tianfu nutritional rice flour carotenoid (not added when special nutritional quality rice is used), grape seed extract (anthocyanin, not added when special nutritional quality rice is used), DVc, sugar and salt vitamins, and the specific content can be between 0.5 and 20 percent.

Optionally adding food-grade galactose, glucose, brown sugar, and inulin as adjuvants.

The raw material of Tianfu TNB culture medium can be 0.5-20% according to different preferences.

The standard for preparing Tianfu TNB culture medium is that the above raw materials can be 0.5-20% according to different preferences, and the vitamins, carotenoid and grape seed extract are lower, and the available proportion is as follows:

(1) corn starch 0-200g

(2) 0-200g of soluble collagen

(3) Soluble elastin 0-200g

(4) Hydrolyzed collagen 0-200g

(5) Ginseng radix extract/gingerol 0-200ml

(6) Hydrolyzed corn protein extract 0-200g

(7) Soybean polypeptide 0-200g

(8) 0-200g of soybean milk powder

(9) 0-200g of milk powder

(10) 0-200g of white sugar

(11) 0-200g Tianfu nutritious rice flour

(12)DVC 0-50g

(13) Edible salt 0-50g

(14) Inulin 0-50g

(15) 0-50g of additives such as various vitamins, nutritional additives and trace elements

(16) Carotenoid and grape extract 0-50g (without nutrition rice or semen Maydis)

(17) 5L of water

Carotenoid and grape extract (without nutrition of rice and corn).

7. Optimum raw material formula for liquid and solid fermentation of Tianfu TNB culture medium

The preparation method of the main material comprises two methods, namely granular fermentation and powder-paste liquid fermentation. The grains are original rice grains, and are cooked for use, and the use ratio is not specially specified, and the grains are required according to hobbies. The powder paste is prepared from rice flour, bean flour and corn flour at random, and the proportion is not limited, and the content can be 0.5-20% according to the situation, and the recommended proportion is 8: 100: 50. Adding 419: 5000 water

By study, the optimal ratio of tianfu TNB medium was as follows:

1) the liquid fermentation culture medium raw materials of the Tianfu TNB culture medium can be flexibly adjusted according to the conditions, the proportion of partial raw materials is increased, or partial raw materials are reduced or even not used, and the optimal raw material proportion is researched as follows:

(1) corn starch 50g

(2) Soluble collagen 50g

(3) Soluble elastin 50g

(4) 50g of hydrolyzed collagen

(5) Ginseng radix extract/gingerol 50ml

(6) Hydrolyzed corn protein extract 50ml

(7) Soybean peptide 50g

(8) Soybean milk powder 50g

(9) Milk powder 50g

(10) 50g of white sugar

(11) Tianfu nutritious rice flour 8g

(12)DVC 5g

(13) Edible salt 5g

(14) Inulin 3g

(15) 1g of additives such as various vitamins, nutritional additives and trace elements

(16) Carotenoid and flavonoid (not using nutritional rice and corn) 0.5g

(17) 5L of water

2) The solid fermentation raw materials of the Tianfu TNB culture medium are as follows:

(1) corn 50g

(2) Soluble collagen 50g

(3) Soluble elastin 50g

(4) 50g of hydrolyzed collagen

(5) Ginseng radix extract/gingerol 50ml

(6) Hydrolyzed corn protein extract 50ml

(7) Soybean peptide 50g

(8) 50g of soybean

(9) Milk powder 50g

(10) 50g of white sugar

(11) Tianfu nutritious rice 8g

(12)DVC 5g

(13) Edible salt 5g

(14) Inulin 3g

(16) Carotenoid and flavonoid (not using nutritional rice and corn) 0.5g

(17) 5L of water

8. Fermentation raw material blending, sterilization and fermentation technical grade

After a fermentation raw material, namely a Tianfu TNB culture medium is prepared, the pH value is generally 6.2-7.0, and the fermentation is carried out under the action of a domestic edible microbial flora (FEMF). The fermentation is divided into 4-6 grades, wherein 1-3 grades are used for sterilizing the raw materials at high temperature and high pressure (above 120 ℃ for more than 20 minutes), and 4-6 grades are used for performing other conventional sterilization such as general boiling sterilization (boiling for 12 minutes) or pasteurization on the raw materials.

The fermentation grade is divided into more than 6 grades, and only 6 grades are written here: the 1-stage fermentation is purified single-bacterium culture and is fermented in a culture bottle; the 2-stage fermentation is the fermentation of flora after the first-stage bacteria are mixed; the 3-stage fermentation is the expanded propagation of the second-stage flora, and can be a simple fermentation tank or a 100-plus-200L fermentation tank. The 4-6 (and 6) stage fermentation belongs to production fermentation, and is carried out by fermenting with 200L or more, such as 1.5 ton or more ton-stage fermenter at 31-37 deg.C, preferably 33 deg.C, at low rotation speed for 36-72 hr, optionally fermenting for one week.

9. Production fermentation technology and process

(1) Grade 1-3 fermentation vessel and Tianfu TNB culture medium, sterilizing with autoclave for 15-25 min, preferably 20 min.

(2) After the 4-6 stage medium was dissolved in water, it was boiled for 12 minutes.

(3) And (4) sterilizing the large container for 30 minutes to 2 hours by using ultraviolet and ozone.

(4) Pouring the sterilized culture medium into a fermentation container, fermenting at 28-39 deg.C and optimal temperature of 32-33 deg.C for 25 hr to 1 week, and optimal time of 48-72 hr.

(5) Primary fermentation, wherein single bacteria are fermented respectively to form pure strains.

(6) And (4) secondary fermentation, wherein the fermented single strains are taken as main materials and are mixed with each other, and 2-5 liters of secondary strains are formed through fermentation.

(7) And (3) performing third-stage fermentation, namely performing 20-200L fermentation on the second-stage fermentation product in a simple fermentation tank or a common fermentation tank by taking 10-20% of seed bacteria as a main body.

(8) And (3) performing four-six-stage amplification and production fermentation, wherein the amplification or production fermentation is performed according to the seed bacteria proportion of 10-20%. Cleaning a fermentation tank before fermentation, and cleaning with tap water once; the surfactant is cleaned once; and (5) washing for 3 times by using tap water, and sterilizing a pipeline and a fermentation tank. Fermenting at an optimal temperature of 32-33 ℃ for 25-72 hours, and optionally prolonging the fermentation time to one week.

10. Simple fermentation equipment

The shape and the structural diagram of the simple fermentation equipment are shown in figure 2:

(1) insulation can: the insulation can is made of wood or iron plate, the electric heating wire is wound on the insulation can, and the insulation can is made of foam board or insulation film, and plays a role in insulation. The size of the box body can be set according to the size of the fermentation vat (see figure 2 and figure 3).

(2) The temperature control heating system is composed of a temperature control system composed of a full-automatic temperature control switch, an electronic thermometer, a heating wire and the like, and is wound outside the heat preservation box, the electronic thermometer is placed between the heating wires, and a heat preservation film is arranged outside the electronic thermometer for heat preservation (see figure 2 and figure 3).

(3)360 vertical bearing revolving stage: a pair of load bearing bearings and brackets of any kind, in the middle of the sides (see fig. 2, fig. 4).

(4) A support platform: the box is supported to ensure stability and smooth 360-degree rotation of the bearing (see fig. 2 and 4).

(5) The fermentation barrels are preferably square and are placed in parallel and vertically. The fermentation barrel can be 5-20L square barrels, such as 2, 4, 6, 8 and 10 square barrels, the number of the barrels can be designed according to requirements, and the number of the barrels is optimally designed to be 4 barrels. A space is provided between the two barrels (see fig. 2, 4).

(6) The central temperature of the equipment is controlled at 32-33 ℃, the revolution can be controlled manually, the fermentation lasts for 25 hours to 1 week, and the optimal time is 48-72 hours.

(7) The rotation speed is not particularly limited, and the rotation speed is not necessarily too high due to anaerobic bacteria fermentation, and the rotation speed is 1-100 circles in 1 hour, and generally 30 circles in 5 hours.

11. Processing, packaging and treating effect

1) And processing and packaging the fermentation product in an aseptic environment to form solid granules and liquid products. The solid granules are generally formed into 5-15g bagged products, and the liquid is formed into 50ml or 100ml small split bottles which are marked with trademarks and production dates.

2) After utilization, the composition exhibits effects in the following aspects

The fermentation reaction product TF120-FEMF has good effect on treating diabetes, and the more serious the condition is, the more obvious the effect is, the more stable the condition is after healing. The best eating method is to eat the food 2 times a day before breakfast and dinner, but the food is used for food therapy. Fasting blood glucose and 2 hours post dinner blood glucose were observed and compared to pre-meal values. After the food is eaten for 28 days, the blood sugar usually returns to normal, and the effect is better when the food is used together with medicaments such as metformin and the like. The patient with serious illness can not stop other medicines, and the patient can not stop the medicines without advice. Stopping taking if blood sugar can not be reduced within 5 days.

3) In addition to diabetes and increased immunity, other effects are found by the product user:

probiotics

(1) Discomfort of the digestive system: has effects in regulating intestinal flora and relieving functional gastrointestinal diseases.

(2) Gastritis: has strong inhibiting effect on helicobacter pylori and good inhibiting effect on biological pathogenic bacteria of gastric cancer.

(3) Enteritis: is beneficial to the recovery of normal gastrointestinal function and the improvement of immunologic function of enteritis of adults and children patients.

(4) Constipation: can be used for adjuvant treatment of functional constipation.

(5) Uric acid: the product contains a large amount of active substances such as natural extracts, probiotics, proteolysis products, polypeptides and the like, and has the effect of reducing uric acid.

(6) The secret urine system: preventing repeated outbreak of urinary tract infection bacterial vaginitis.

Description of the drawings:

FIG. 1 shows that the improved Tianfu orange red corn with orange red seed coat obtained by conventional breeding contains not only zeaxanthin but also carotenoids yet undetected, such as lutein and beta-carotene.

FIG. 2 is a view showing the shape and structure of a simple fermentation apparatus

Fig. 3 shows that the thermal insulation box is made of wood or iron plates, heating wires are wound on the thermal insulation box, and thermal insulation materials such as foam boards or thermal insulation films are wrapped outside the thermal insulation box to achieve the thermal insulation effect. The temperature control heating wire is wound outside the box body by a temperature control system consisting of a full-automatic temperature control switch, an electronic temperature measuring instrument, a heating wire and the like, the electronic temperature measuring instrument is arranged between the heating wires, and a heat preservation film is arranged outside the electronic temperature measuring instrument for preserving heat.

FIG. 4 shows that the fermentation barrels are preferably square, are placed side by side in a vertical manner, and are 5-20L, and the number of the fermentation barrels is preferably 4, and a space is arranged between the two barrels. The bearing rotary table is arranged in the middle and can rotate 360 degrees, and the support table plays a role in supporting equipment.

FIG. 5 without any other drugs, only TF120-FEMF was taken, and after 17 days, the postprandial blood glucose decreased from 17.4 to 6.5 in volunteers; fasting blood glucose becomes normal.

Figure 6. body mass index ═ (body weight-73) × 2; mental index ═ 100-speech age)/4; whether the medicine is taken or not: 1 is taken; no is 0; fasting blood glucose; administering TF120-FEMF viable bacteria solution for 1-9 days; the TF120-FEMF after boiling treatment is taken for 10-17 days, and the effect is the same.

Detailed Description

Example 1

1. Food-borne edible microbial flora (FEMF) and staple food grain prebiotic fermentation product for treating diabetes

The product prepared by the technology according to the method in the research content has good effect for patients with diabetes and hypertension of 40 years old king x (figure 5), and the patients only drink TF 120-FEMF!without any other medicine! After eating the product of the technology for 17 days, the fasting blood sugar of wang x is stably and normally reduced from unstable 10.5 to 6.3, and is reduced from 17.5 to 6.5 after 2 hours, and the key is glycosylated hemoglobin, which is reduced from 9.2 to 8.8 before hospital admission. The domestic edible microbial flora (FEMF) and the main grain prebiotics fermentation product have obvious effect on treating the diabetes.

Example 2

The special nutritional quality rice Tianfu 106, Tianfu 206, Tianfu 409, Tianfu 307 and the like which are used as main grains of the culture medium have better effect than that of common rice and corn. The breeding method of the nutrient staple food grain comprises the steps of firstly screening a large number of parents containing carotenoid and flavonoid, obtaining the parent Tianfuqing 2 through breeding, and then hybridizing the parent Tianfuqing 2 with local rice to obtain the rice and the corn with the characteristic nutrient quality of carotenoid, anthocyanin and the like. For example, Tianfu red corn, which is rice Tianfu 106, Tianfu 206, Tianfu 409 and Tianfu 307, contains carotenoids and anthocyanins, and orange and red corn kernels, contains lutein, beta-carotene and the like in addition to zeaxanthin. The soybean is not high in requirement, and instant soybean powder can be used, and black soybean powder and soybean powder can be used.

By using the special nutrient rice and corn, the method can make the first stepWithout any other medicine40 years old patient, with a postprandial blood glucose drop from 17.4 to 6.5 within 17 days; ② canThe long-acting insulin can be used for treating the serious diabetes of 30 years old who has poor western medicine effect after being rescued for 2 times and has heart disease and hypoxia within 23 daysAll are out of useThe glycosylated hemoglobin is reduced from 9.6 to 8.8, and the fasting blood sugar is reduced from 22.1 to discharge; ③ the 23-year-old hereditary type II diabetes of 62 years old who is rapidly emaciating can be treated by insulin for 16 years, the complications are coronary heart disease, hypertension (extremely high risk), cerebral infarction and myocardial infarction, and the mother of the patient dies from diabetes in the age of 59 years old and eats the food3 sky belly blood sugarFrom 14.2 to 8.2 and 7.3 to normal 6.5, 6.2 and 6.8 …,after 28 days of treatment, the fasting blood sugar is normal and stableWeight gain, appetite, sleep and mental well-being. Fourthly, 83-year-old people without other medicines have fasting blood glucose for 3 months from 17.5And (5) recovering to be normal.

Example 3

The domestic edible microbial flora (FEMF) has wide utilization and good effect, has the edible fungus agent for treating metabolic syndrome such as diabetes and the like and improving the immunity, is cultivated and eaten by 90-year-old people at home 50 years ago, and the main effective fungi comprise: bacillus, various lactic acid bacteria and bifidobacteria. Under the condition of no auxiliary improvement bacteria, 100 percent FEMF is fermented by a Tianfu TNB culture medium liquid and solid fermentation culture medium, the components of each culture medium in an early staple food grain prebiotics fermentation culture medium also called a Tianfu TNB culture medium formula are random and are different from 0 to 200g, the optimal raw material proportion and the optimal formula are gradually determined to carry out 1-6-stage fermentation, and 4, 5 and 6-stage industrialized production fermentation is formed to produce liquid and solid products.

After the fermented product is eaten, diseases in the epidemic situation period of 5 months in 2020 are cured: 83 year old mother often dizziness and heart pain because of diabetes (fasting blood sugar 17.5), then epidemic situation is nervous and dare to go to hospital, no medical advice is dare to take medicine, and the nutritional rice-Tianfu 120(TF120) is used to prepare fermentation product (TF120-FEMF) under the action of domestic edible microorganism flora (FEMF) and auxiliary materials under the exigency to try to improve the metabolic syndrome. The result is that TF120-FEMF administration is almost immediate! In addition, the blood sugar of the 83-year-old man king xx (150 cm in height, 140 jin in weight and 5 years in diabetes history) before meals can be reduced from 17.5 to 7, so that symptoms of cardiac pain, dizziness and hypertension disappear, after taking TF120-FEMF for one month, the blood sugar is relatively stable, under the condition of not taking any medicine, a patient after 3 months can be basically cured, fruits can be eaten frequently, and all diabetes and complications disappear magically. Meanwhile, the immunity of the organism is improved, the disease resistance of the body is strong after the use, the common cold and arthralgia in the past are avoided, the immunity is improved, and the energy becomes vigorous.

EXAMPLE 4 simple fermentation apparatus

Simple fermentation equipment provides a great deal of convenience for the application of the technology. The food therapy treatment of the fermentation product TF120-FEMF is realized! The medicine is interdisciplinary, comprises mechanical and agricultural rice, microbial flora, metabolic synthesis, molecular biology and the like, has quick and good treatment effect on the old and the family of long-term and serious young people for diabetes mellitus, is stable after healing, and improves the immunity of patients.

The simple fermentation equipment can ferment 1-4 square barrels of 5-20L, and the single machine can ferment 5-80L of fermentation products at one time. The fermentation can be carried out at 32-33 deg.C and 21-45 deg.C, the rotation number can be controlled manually, the rotation time is 1-100 circles, generally 5 hours to 30 circles, the fermentation time is 25 hours to 1 week, and the optimal time is 48-72 hours. The simple fermentation equipment is very convenient and has strong applicability in the production of 3, 4 and 5-level microbial agents and even the production within 200L of higher generation.

Example 5 domestic microbial flora (FEMF) in combination with helper bacteria

In the case of 50% use of the family-borne edible microbial flora (FEMF), any three to all of the following bacteria, added to 0-20%, are effective:

(1) the using proportion of the bacillus natto is set to be 0-20%.

(2) The use ratio of the bacillus licheniformis is set to be 0-20%.

(3) The use ratio of the bacillus amyloliquefaciens is set to be 0% to 20%.

(4) The use ratio of the bacillus subtilis is set to be 0-20%.

(5) The streptococcus thermophilus is set to be used in a proportion of 0% to 20%.

(6) The lactobacillus plantarum is set to be used in a proportion of 0% to 20%.

(7) The lactobacillus rhamnosus is set to be used in a proportion of 0 to 20 percent.

(8) Lactobacillus casei 0-20%.

(9) Lactobacillus paracasei 0% to 20%.

(10) Lactobacillus acidophilus 0% to 20%.

Any random combination of the above-mentioned bacteria with FEMF bacteria is effective.

Example 6 FEMF Master-Effect bacteria/auxiliary improving bacteria ratio

The FEMF major bacteria comprise: the bacillus, various lactic acid bacteria and bifidobacteria are generally used by 80-100 percent, and the domestic edible microorganism flora (FEMF) main effective bacteria by 100 percent can be used without any auxiliary bacteria, if the auxiliary improvement is added, the optimal proportion is as follows:

(1) 80% of domestic edible microbial flora (FEMF) main effective bacteria.

(2) The using proportion of the bacillus natto is set to be 2%.

(3) The use ratio of the bacillus licheniformis is set to be 2%.

(4) The use ratio of the bacillus amyloliquefaciens is set to be 2%.

(5) The use ratio of the bacillus subtilis is set to be 2%.

(6) Streptococcus thermophilus was used at a rate of 2%.

(7) The lactobacillus plantarum was used at a ratio of 2%.

(8) The lactobacillus rhamnosus is set to be used in a proportion of 2 percent.

(9) Lactobacillus casei was used at a ratio of 2%.

(10) The ratio of lactobacillus paracasei to be used was set to 2%.

(11) Lactobacillus acidophilus was used at a rate of 2%.

Example 7 solid granules and liquid products have good therapeutic effects on familial hereditary long-term type II diabetes with multiple illnesses

1) And (4) processing and packaging the fermentation product in an aseptic environment to form the fermentation product.

The solid granules and the liquid products have good treatment effect on weak and sick people. The male has suffered from type II diabetes for 23 years (family history), the volunteer king xx who has been injected with insulin for 15-16 years is 62 years old, the type II diabetes causes coronary heart disease, hypertension (extremely high risk), cerebral infarction and myocardial infarction, the recent emaciation begins, gastroenteritis, constipation, anorexia, listlessness, insomnia, uric acid, and hypodynamia and fatigue of the micturition system, and the speaking voice is very old like 90 years old and has weakness. Insulin is being administered, metformin and aspirin are being consumed, and fasting blood glucose is 14.2 in the morning.

The patient often has the following diseases:

(1) the digestive system is uncomfortable, the stomach is gastritis, and the appetite is often poor.

(2) Enteritis and constipation.

(3) Uric acid, and diseases of the urinary system.

By using TF120-FEMF for treatment, a volunteer has hunger feeling after taking the food for 2 hours, has good appetite and more food, eats a large bowl of Japanese plum mr beef noodles in the noon on the day and a bowl of rice and a plurality of vegetables at night, changes the habit of bad sleep, and has deep and stable sleep, long time and vigorous energy. The fasting blood sugar of 8.2 in the morning of the next day, no other bad feelings exist in the body, and the body weight is slightly increased (156 jin) in the evening of the next day; on the third day, the fasting blood sugar is 7.3, the patient feels very good, other functions of the body are also improved, half a cake in breakfast is two eggs, eight beef in noon is roasted, one bowl of returning people is added, and the people are mixed and too much is eaten. Eating half a kilogram of sweet potato at night and assisting with a bowl of corn; on day four the patient says that blood glucose is 6.5! The weight is slightly increased (158 jin)! 6.5 near Normal level! Patients should have been 6.5 out for 23 years. Blood glucose 6.2 on the fifth day and 6.8 on the seventh day. In 23 years of type 2 diabetes, 16 years of insulin is taken, the weight is sharply reduced, and myocardial infarction, coronary heart disease and cerebral infarction hypertension are extremely dangerous, the blood sugar becomes normal after the TF120-FEMF is treated for 3 consecutive days! The symptoms of gastritis and enteritis disappear, the habit of poor sleep is changed, the sleep is deep and stable, the time is very long, the diseases of uric acid and the secret urine system disappear, and the energy becomes vigorous.

Volunteers rarely have the state of refreshing and refreshing for 23 years continuously, and the mental state can be reflected by speaking mood. Blood sugar is continuous and normal, sleep is good, weight is stable, and the user is energetic and has powerful voice (as a parameter, (100-voice age)/4 ═ energy index). On the eighth day volunteers started to dating to the college classmates and were considered to be diabetic, not having to drink TF 120-FEMF! The blood sugar rises steeply by 8.7!on the ninth day! The volunteers are listened to and have qi weakness. Fasting blood glucose was 5.7 after TF120-FEMF treatment was taken at night. On the tenth day, the dry powder (perhaps inactivated) of the solid electuary was taken and blood glucose remained relatively stable within the normal range. This clearly shows that TF120-FEMF treatment is very effective both in live solution and after hyperthermic treatment! The recovery of health was very obvious by clinical examination of volunteers from other relatives and friends, and in fig. 6, the body mass index is (body weight-73) × 2; mental index ═ 100-speech age)/4. Whether the medicine is taken or not: 1 is taken; no is 0; fasting blood glucose. Administering TF120-FEMF viable bacteria solution for 1-9 days; the TF120-FEMF after boiling treatment is taken for 10-17 days, and the effect is the same.

After the volunteer takes the food for 28 days, the blood sugar before meal is stable and is always between 6.1 and 6.8 after another 22 days of observation, and perhaps the habit is formed by normalization of blood sugar. Occasionally, the special condition fluctuates slightly when more sugar is consumed. Other volunteers such as 61 years old Uxx: diabetes, pulmonary nodule, coronary heart disease, hypertension, and cerebral infarction. If people are X at age 64, the treatment effect of the patients with diabetes and hypertension is good, and the details are not repeated. If 1.14 million diabetics nationwide and 4.25 million diabetics worldwide can be treated by the research, the research brings good news to the diabetics.

Example 8 anti-hangover Effect of gastrointestinal distress after drinking

The solid granule and liquid product have effects of relieving hangover and protecting gastrointestinal tract. The discomfort of drinking the product after drinking is relieved after about 3 hours. The product has a certain relation with the nutritional staple food grain prebiotics containing functional microbial inoculum and plant secondary metabolites.

Table 1.26 year II diabetic patients take a 0-17 day record table:

in summary, the edible fungus preparation and the staple food grain prebiotics for treating diabetes and improving immunity and the production technology thereof disclose a main material, an auxiliary material, a seasoning, an edible fungus and fermentation equipment of a staple food grain prebiotics culture medium which is also called a Tianfu TNB culture medium. The edible fungus is a family-borne edible microorganism flora (FEMF) and its improved auxiliary fungus, and a preparation technology of fermented products. The selection and breeding method of staple food grains in the staple food grain prebiotics culture medium are explained. For example, the special nutritional rice quality Tianfu 106, Tianfu 206, Tianfu 409 and Tianfu 307, and the breeding method of the nutritional corn with orange-red seed coat are characterized in that the seed coat contains carotenoid which is not detected yet, such as lutein, beta-carotene and the like besides zeaxanthin.

The family-wide edible microbial flora (FEMF) is complex and includes: bacillus, various lactic acid bacteria, bifidobacterium and the like. The microbial inoculum presents sour and sweet taste after fermentation, gives the structures and the proportion of improved FEMF flora and auxiliary flora, and is suitable for the formula of a staple food grain prebiotics fermentation culture medium also called Tianfu TNB culture medium, including the optimal raw material formula of liquid and solid fermentation.

The blending and sterilization of fermentation raw materials and the 1-6 level fermentation technology are explained, and simple fermentation equipment is designed and manufactured.

The fermentation reaction product TF120-FEMF has good effect on treating diabetes, and the more serious the condition is, the more obvious the effect is, the more stable the condition is after healing. The best eating method is to eat the food 2 times a day before breakfast and dinner, but the food is used for food therapy. Usually takes effect after 5 days, and usually returns blood sugar to normal after 28 days.

Besides the functions of treating diabetes and improving immunity, the product of the technology finds other effects:

Claims

1. The technology comprises main materials, auxiliary materials and seasonings of a fermentation culture medium, the edible fungi, simple fermentation equipment and industrial production tonnage fermentation equipment, wherein the edible fungi are family-borne edible microorganism flora (FEMF) and improved auxiliary fungi thereof, and the main grain prebiotics culture medium is also called a fermentation technology of Tianfu TNB culture medium and a fermentation product liquid and solid preparation technology.

2. The selection and breeding method of staple food grain in the staple food grain prebiotic culture medium according to claim 1.

3. The composition of the species of the family-born edible microorganism flora (FEMF) for the treatment of metabolic syndrome such as diabetes mellitus and the improvement of immunity according to claim 1.

4. The modified FEMF population and helper population of claim 1, having a structure and ratio.

5. The staple food grain prebiotic fermentation medium of claim 1 is also referred to as a formulation of Tianfu TNB medium.

6. The optimum feedstock formulation for liquid and solid state fermentation of Tianfu TNB medium as claimed in claim 1.

7. The fermentation feedstock formulation, sterilization and 1-6 stage fermentation technology of claim 1.

8. The industrial production 4-6 grade fermentation technology and product process according to claim 1.

9. The simple fermentation device of claim 1.

10. The method and effect of claim 1.