CN113243385B

CN113243385B - Pesticide composition and application thereof

Info

Publication number: CN113243385B
Application number: CN202110549444.0A
Authority: CN
Inventors: 余思策; 刘润峰; 陶代雨; 张磊; 熊利鹏; 赵悬豪; 李光亮
Original assignee: Pilarquim Shanghai Co ltd
Current assignee: Pilarquim Shanghai Co ltd
Priority date: 2021-05-20
Filing date: 2021-05-20
Publication date: 2022-11-01
Anticipated expiration: 2041-05-20
Also published as: CN113243385A

Abstract

The invention relates to the field of pesticides, and particularly relates to a pesticide composition and application thereof. The active ingredients of the pesticide composition contain prothioconazole, pyraclostrobin and difenoconazole, and the active ingredients of the pesticide composition are prothioconazole, pyraclostrobin and difenoconazole. The ternary compound medicament of the prothioconazole, the pyraclostrobin and the difenoconazole has a synergistic effect, obviously improves the control effect on diseases compared with the use of a single agent or a binary compound medicament, and enlarges the control spectrum.

Description

Pesticide composition and application thereof

Technical Field

The invention relates to the field of pesticides, and particularly relates to a pesticide composition and application thereof.

Background

In agricultural production, there are often problems with simultaneous mixing or sequential occurrence of different types of diseases, such as powdery mildew, scab, leaf rust, banded sclerotial blight, leaf spot, net blotch, gray mold, black spot, brown spot, black shank, downy mildew, black pox, anthracnose, white rot, which often occurs on wheat, barley, grapes, bananas, apples, cucumbers and peanuts. The effective components of the existing disease control medicament are single, so that the target is easy to generate drug resistance, the drug effect is poor, and meanwhile, different medicaments are required for different diseases, so that a large amount of manpower and financial resources are consumed.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a pesticide composition, which contains active ingredients, wherein the active ingredients comprise prothioconazole, pyraclostrobin and difenoconazole, and the weight ratio of the prothioconazole, the pyraclostrobin and the difenoconazole is 1: (0.1-10): (0.1 to 10); optionally, the weight ratio is 1: (0.2-5): (0.2 to 5); further optionally, the weight ratio is 1: (0.5-1.5): (0.5-1.5).

Optionally, the pesticide composition further comprises an auxiliary agent, wherein the auxiliary agent is one or more of a wetting agent, a dispersing agent, an emulsifier, a solvent, a cosolvent, a thickening agent, a stabilizer, an antifreezing agent, a defoaming agent, a preservative, a binder, a wall material, an acid adjusting medium, an alkali adjusting medium and a solid inert carrier.

Optionally, the pesticide composition is in the form of one or more of a suspending agent, a seed treatment suspending agent, a suspoemulsion, a microcapsule suspending agent, an aqueous emulsion, a dispersible oil suspending agent, a wettable powder or water dispersible granules.

The second purpose of the invention is to provide the application of the composition in preventing and controlling crop diseases.

Optionally, the disease in the crop comprises one or more of powdery mildew, gibberellic disease, leaf rust, banded sclerotial blight, leaf spot, net blotch, gray mold, black spot, brown spot, black shank, downy mildew, anthracnose, and white rot.

Optionally, the crop comprises one or more of wheat, barley, grapes, bananas, apples, cucumbers and peanuts.

The invention has at least the following beneficial effects:

the ternary compound medicament of the prothioconazole, the pyraclostrobin and the difenoconazole has a synergistic effect, and the prevention and treatment effect on diseases is obviously improved compared with the use of a single agent or a binary compound medicament.

The ternary compound pesticide of prothioconazole, pyraclostrobin and difenoconazole can prevent and treat multiple diseases occurring simultaneously or in succession by one-time application, so that the use amount of the pesticide is reduced, the use cost is reduced, and the promotion of disease resistance is delayed.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In some embodiments, materials, elements, methods, means, and the like that are well known to those skilled in the art are not described in detail in order to not unnecessarily obscure the present invention.

The embodiment of the invention provides a pesticide composition for preventing and treating one or more plant diseases of powdery mildew, scab, leaf rust, banded sclerotial blight, leaf spot, net blotch, gray mold, black spot, brown spot, black shank, downy mildew, anthracnose, white rot. The prothioconazole is a novel broad-spectrum triazolethione bactericide, and the action mechanism of the prothioconazole is to inhibit the demethylation action of a sterol precursor in fungi, namely lanosterol or 24-methylene dihydro MI Mao Zui l4 position, namely demethylation inhibitors (DMIs). Not only has good systemic activity, excellent protection, treatment and eradication activity, but also has long lasting period. Pyraclostrobin is a novel broad-spectrum methoxy acrylic acid snore bactericide, can finally cause cell death by inhibiting mitochondrial respiration, and has the functions of protection, treatment and leaf penetration and conduction. The difenoconazole belongs to triazole bactericides, is a sterol demethylation inhibitor, has the characteristics of high efficiency, broad spectrum, low toxicity and low dosage, is an excellent variety of the triazole bactericides, has strong systemic property, and can destroy the cell membrane structure and function of pathogenic bacteria by inhibiting the biosynthesis of ergosterol of pathogenic bacteria cells.

Wherein the weight ratio of prothioconazole, pyraclostrobin and difenoconazole in the pesticide composition is 1: (0.1-10): (0.1 to 10); optionally, the weight ratio is 1: (0.2-5): (0.2 to 5); further optionally, the weight ratio is 1: (0.5-1.5): (0.5-1.5). More preferably 3:3:2. 2:3: 3. 3:2:3.

the pesticide composition of the embodiment of the invention comprises 0.1-40 wt% of prothioconazole, preferably 1-30 wt%, and more preferably 10-20 wt%;

the pesticide composition of the embodiment of the invention comprises 0.1-40 wt% of pyraclostrobin, preferably 1-30 wt%, and more preferably 10-20 wt%;

the weight percentage of the difenoconazole in the pesticide composition of the embodiment of the invention is 0.1-40%, preferably 1-30%, and more preferably 5-15%.

And further preferably: 15% of prothioconazole, 15% of pyraclostrobin and 10% of difenoconazole; or 10% of prothioconazole, 15% of pyraclostrobin and 15% of difenoconazole; or, prothioconazole 15%, pyraclostrobin 10% and difenoconazole 15%.

The pesticidal composition of the present invention may be provided in the form of a finished product, i.e. the ingredients of the composition have been mixed; each component can also be provided in separate formulations for direct tank mixing at the time of use.

Optionally, the content of the active ingredient in the pesticide composition of the embodiment of the invention is 1% to 95%, and in the invention, there is no special requirement on the total content of the active ingredient in the bactericide composition, and the conventional dosage can be selected according to different formulations prepared from the bactericide composition, which is well known to those skilled in the art. In addition to the active substance, an auxiliary agent is also included; the auxiliary agent is one or more of a wetting agent, a dispersing agent, an emulsifying agent, a solvent, a cosolvent, a thickening agent, a stabilizing agent, an antifreezing agent, a defoaming agent, a preservative, a binder, a wall material, an acid adjusting medium, an alkali adjusting medium and a solid inert carrier. The content of the active ingredient in the pesticide composition of the embodiment of the invention is preferably 25-55%, more preferably 40%, and the active ingredient and the components are auxiliaries. Within this ratio range, a preparation containing the pesticidal composition having more stable properties can be prepared.

In the auxiliary agent required by the pesticide composition, the dispersing agent comprises one or more of alkyl naphthalene sulfonate, a bis (alkyl) naphthalene sulfonate formaldehyde condensate, a polyoxyethylene polyoxypropylene block copolymer, aryl phenol polyoxyethylene succinate sulfonate, octyl phenol polyoxyethylene ether sulfate, polycarboxylate, sodium lignosulfonate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, sodium naphthalene sulfonate formaldehyde condensate, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, fatty acid polyoxyethylene ester and ester polyoxyethylene ether.

In the auxiliary agents required by the pesticide composition, the wetting agent comprises one or more of lauryl sodium sulfate, sodium dodecyl benzene sulfonate, nekal BX, pesticide emulsion 2000# series, sodium lauryl sulfate, wetting penetrating agent F, wetting agent T, chinese honeylocust fruit powder, sasangua cake, silkworm excrement and soapberry powder.

In the auxiliary agents required by the pesticide composition, the solvent comprises one or more of methanol, ethanol, propanol, isopropanol, propylene glycol, glycerol, n-butanol, isobutanol, tert-butanol, n-pentanol, isoamyl alcohol, n-hexanol, n-heptanol, n-octanol, ethylene glycol monomethyl ether, ethylene glycol dimethyl ether, sorbitol, aromatic hydrocarbon solvent oil and methyl oleate.

In the auxiliary agents required by the pesticide composition, the cosolvent comprises one or more of methanol, isopropanol, dimethyl sulfoxide, N-methyl pyrrolidone, sulfolane and ethyl acetate.

In the auxiliary agents required by the pesticide composition, the antifreezing agent comprises one or more of ethylene glycol, 1,2-propylene glycol, glycerol and urea.

In the adjuvant required by the pesticide composition, the binder comprises one or more of starch, gypsum, glucose, polyvinyl alcohol, gum arabic and dextrin.

In the auxiliary agents required by the pesticide composition, the thickening agent comprises one or more of magnesium aluminum silicate, xanthan gum, polyethylene glycol, polyvinyl alcohol, carboxymethyl cellulose and phenolic resin.

In the auxiliary agent required by the pesticide composition, the solid inert carrier comprises one or more of clay, silicon dioxide, diatomite, kaolin, attapulgite, montmorillonite, bentonite, talcum powder and light calcium carbonate.

In the auxiliary agents required by the pesticide composition, the defoaming agent comprises silicone and C_8-10Fatty alcohols, C_10-20Saturated fatty acids (such as capric acid) and one or more of amide, silicone oil and silicone compounds.

In the auxiliary agent required by the pesticide composition, the wall material comprises one or more of polyurea, polyurethane, melamine resin, mixed polyurea/polyamide, gelatin/Arabic gum, polyamide, urea/formaldehyde, nylon (Nylon) and polyethylene/paraffin.

In the auxiliary agents required by the pesticide composition, the acid adjusting medium comprises one or more of ammonium chloride, hydrochloric acid, citric acid, ammonium sulfate, ammonium nitrate and acetic acid.

In the auxiliary agent required by the pesticide composition, the alkali adjusting medium comprises one or more of sodium hydroxide, triethanolamine, triethylamine, ammonia water and sodium ethoxide.

Among the adjuvants required for the pesticidal composition of the present invention, stabilizers and preservatives are those commonly used in the art, which are well known to those skilled in the art and will not be described herein.

The pesticide composition is in the dosage form of one or more of suspending agent, seed treatment suspending agent, suspending emulsion, microcapsule suspending agent, aqueous emulsion, dispersible oil suspending agent, wettable powder or water dispersible granule;

optionally, the suspending agent comprises 3 to 60 parts by weight of active ingredients, 1 to 10 parts by weight of dispersing agents, 0.1 to 5 parts by weight of wetting agents, 0.1 to 10 parts by weight of antifreeze agents, 0 to 10 parts by weight of other additives and 0 to 10 parts by weight of water; other additives include one or more of stabilizers, preservatives, thickeners, or defoamers. Wherein, the dispersant is preferably sodium lignosulfonate, and the thickener is preferably xanthan gum.

The embodiment of the invention also relates to the application of the pesticide composition in preventing and treating one or more plant diseases of powdery mildew, scab, leaf rust, banded sclerotial blight, leaf spot, net blotch, gray mold, black spot, brown spot, black shank, downy mildew, anthracnose and white rot, wherein crops comprise grain crops and cash crops; the grain crops mainly refer to cereal crops (such as wheat, rice and corn), and also include potato crops (including sweet potato, potato and the like) and bean crops (including soybean, broad bean, pea, mung bean and the like). Economic crops comprise oil crops, vegetable crops, fruit trees and the like; wherein the control effect on gramineae plants, vitaceae plants, rosaceae plants, cucurbitaceae plants, musaceae plants and Leguminosae plants is better; experiments prove that the composition has definite control effect on wheat, barley, grapes, bananas, apples, cucumbers and peanuts, and has obvious synergistic effect. The dosage of the active ingredients in application is 5 to 10 grams per mu, preferably 8 grams per mu. The application of the embodiment of the invention can reduce the usage amount of the pesticide and reduce the pesticide cost.

The following describes in detail specific embodiments of the present invention. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are given by way of illustration and explanation only, not limitation.

Example 1

Preparation of 15% prothioconazole, 15% pyraclostrobin, 10% difenoconazole suspending agent

The composition comprises, by mass, 15% of prothioconazole, 15% of pyraclostrobin, 10% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Example 2

Preparation of 10% prothioconazole, 15% pyraclostrobin, 15% difenoconazole suspending agent

The composition comprises, by mass, 10% of prothioconazole, 15% of pyraclostrobin, 15% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Example 3

Preparation of 15% prothioconazole, 10% pyraclostrobin, 15% difenoconazole suspending agent

The composition comprises, by mass, 15% of prothioconazole, 10% of pyraclostrobin, 15% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Example 4

Preparation of 30% prothioconazole, 5% pyraclostrobin, 5% difenoconazole suspending agent

The composition comprises, by mass, 30% of prothioconazole, 5% of pyraclostrobin, 5% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Example 5

Preparation of 5% prothioconazole, 30% pyraclostrobin, 5% difenoconazole suspending agent

The composition comprises, by mass, 5% of prothioconazole, 30% of pyraclostrobin, 5% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Example 6

Preparation of 5% prothioconazole, 5% pyraclostrobin and 30% difenoconazole suspending agent

The composition comprises, by mass, 5% of prothioconazole, 5% of pyraclostrobin, 30% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Example 7

Preparation of 18% prothioconazole, 14% pyraclostrobin, 8% difenoconazole Wettable Powder (WP)

The pesticide composition is prepared by uniformly mixing 18% of prothioconazole, 14% of pyraclostrobin, 8% of difenoconazole, 6% of sodium methylenedinaphthalene sulfonate, 2% of sodium dodecyl sulfate, 3% of white carbon black and kaolin in parts by mass, and then crushing the mixture to 325 meshes by using an airflow crusher to obtain the pesticide composition.

Example 8

Preparation of 16% prothioconazole, 16% pyraclostrobin, 8% difenoconazole Water Dispersible Granule (WDG)

The pesticide composition is prepared by mixing 18% of prothioconazole, 14% of pyraclostrobin, 8% of difenoconazole, 5% of acrylic acid- (methyl) acrylate copolymer, 3% of sodium dodecyl sulfate, 5% of sucrose and ammonium sulfate in percentage by mass, supplementing, uniformly mixing, crushing, granulating, sieving and drying.

Comparative example 1

Preparation of 40% propiconazole suspending agent

The composition comprises, by mass, 40% of prothioconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is water.

Comparative example 2

Preparation of 40% pyraclostrobin suspending agent

The components are 40% of pyraclostrobin, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol in percentage by mass, and the balance is made up by water.

Comparative example 3

Preparation of 40% difenoconazole suspending agent

The composition comprises, by mass, 40% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of silicone defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Comparative example 4

Preparation of 20% prothioconazole-20% pyraclostrobin suspending agent

The composition comprises, by mass, 20% of prothioconazole, 20% of pyraclostrobin, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of organic silicon defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Comparative example 5

Preparation of 20% prothioconazole and 20% difenoconazole suspending agent

The composition comprises, by mass, 20% of prothioconazole, 20% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of silicone defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

Comparative example 6

Preparation of 20% pyraclostrobin 20% difenoconazole suspending agent

The composition comprises, by mass, 20% of pyraclostrobin, 20% of difenoconazole, 2% of sodium lignosulfonate, 2% of polyoxyethylene polyoxypropylene block copolymer, 2% of polycarboxylate, 0.12% of xanthan gum, 0.3% of silicone defoamer, 0.2% of sodium benzoate and 4% of ethylene glycol, and the balance is made up by water.

The contents of the respective active ingredients in the examples and comparative examples are shown in table 1:

TABLE 1

	Prothioconazole (%)	Pyraclostrobin (%)	Difenoconazole (%)
				Example 1	15	15	10
Example 2	10	15	15
				Example 3	15	10	15
Example 4	30	5	5
				Example 5	5	30	5
Example 6	5	5	30
				Example 7	18	14	8
Example 8	16	16	8
				Comparative example 1	40	—	—
Comparative example 2	—	40	—
				Comparative example 3	—	—	40
Comparative example 4	20	20	—
				Comparative example 5	20	—	20
Comparative example 6	—	20	20

Test example 1

The test example is experimentally set in Shanghai Fengxian district, the crop to be tested is wheat, and the variety is Yangmai 158. The land plot has flat terrain and even land capability, and the wheat in each treatment area is normally managed after planting.

The experiment was conducted with a total of 13 treatments, examples 1-6, comparative examples 1-6 and a clear water control, each treatment was repeated 4 times for a total of 52 cells each having an area of 15m²The effective component dosage is 8 g/mu (clear water is not applied in the control plot) for spraying treatment, random block arrangement is adopted among plots, and the seeding rate is 15 kg/mu.

According to the growth process of wheat, the control effects of different treatments on wheat powdery mildew, wheat scab and wheat leaf rust are investigated at 14d after the application of the pesticide, and the specific statistical method is as follows:

the method for investigating wheat powdery mildew comprises the following steps: five-point sampling per diagonal of test cell, 0.25m per survey point²Wheat plants and wheat jointing stage survey 1-5 leaves at the base, after ear emergence survey flag leaves of each plant and the first leaf below the flag leaves, the total leaf number, the diseased leaf number and the disease grade number are recorded, and the control effect is calculated according to the following formulas (formula 1 and formula 2).

Wheat powdery mildew disease grading index (in leaves): level 0: no disease; level 1: the area of the lesion spots accounts for less than 5% of the whole area; and 3, level: the area of the lesion spots accounts for 6 to 15 percent of the whole area; and 5, stage: the area of the lesion spots accounts for 16 to 25 percent of the whole area; and 7, stage: the area of the lesion spots accounts for 26 to 50 percent of the whole area; and 9, stage: the area of the lesion spots accounts for more than 50 percent of the whole area.

The investigation method of wheat scab comprises the following steps: in the wax ripening period of stable wheat scab, sampling investigation is carried out at 5 points of diagonal lines of a cell, the disease level of each ear is recorded by investigating 100 ears at each point, the number of diseased ears and the disease level are recorded, and the control effect is calculated according to the following formulas (formula 1 and formula 2)

Grading index of wheat scab disease: level 0: the whole spike is disease-free; level 1: the disease of spikelets below 1/4 of the whole spike; and 3, level: 1/4-1/2 spikelets of the whole spike are attacked; and 5, stage: 1/2-3/4 spikelets of the whole spike are attacked; and 7, stage: the disease is caused by more than 3/4 of the whole ear.

The method for investigating the wheat leaf rust comprises the following steps: sampling 5 points per cell, investigating 50 stems per point, recording disease grade of flag leaf and inverted 2 leaves per stem, investigating 100 leaves per point, recording disease leaf number and disease grade number, and calculating the preventing and treating effect according to the following formulas (formula 1 and formula 2)

Grading index of wheat leaf rust disease condition: level 0: no disease; level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf; and 3, level: the area of the lesion spots accounts for 6 to 25 percent of the area of the whole leaf; and 5, stage: the area of the lesion spots accounts for 26 to 50 percent of the area of the whole leaf; and 7, stage: the area of the lesion spots accounts for 51 to 75 percent of the area of the whole leaf; and 9, stage: the lesion spot area accounts for more than 76% of the whole leaf area.

In the formula: CK (CK)₀-pre-application disease index in the control zone; CK (CK)₁-disease index after application to the clear water control area; PT₀-pre-dose disease index in the agent treatment area; PT₁-disease index after administration to the agent treatment area.

By comparing the most preferred examples with comparative examples 1 to 6, the combined action pattern of prothioconazole, pyraclostrobin and difenoconazole in the treatment of wheat powdery mildew, wheat scab and wheat leaf rust was evaluated by the Gowing method.

Calculating the formula: cexp = C1+ C2 (1-C1), C1 and C2 are control effects when the bactericides A and B are used independently, cexp is a theoretical control effect of the bactericides A and B, and Cabs is an actual control effect of the bactericides A and the bactericides B after being mixed according to the proportion. Cabs & gt, cexp, are synergistic, cabs & lt, cexp, are antagonistic.

TABLE 1 control effect on wheat diseases 14 days after treatment with different agents

TABLE 2 Combined Effect of prothioconazole in combination with pyraclostrobin and difenoconazole on wheat powdery mildew, wheat scab and wheat leaf rust

TABLE 3 Combined action of pyraclostrobin in combination with prothioconazole and difenoconazole on wheat powdery mildew, wheat scab and wheat leaf rust

TABLE 4 Combined Effect of Difenoconazole with prothioconazole and pyraclostrobin combinations on wheat powdery mildew, wheat scab, wheat leaf rust

Test example 2

The test example is experimentally set in Huaian city of Jiangsu province, the plot is smooth in terrain and uniform in land fertility, and the cucumbers in each treatment area are normally managed after planting.

The experiment was conducted with a total of 13 treatments, examples 1-6, comparative examples 1-6 and a clear water control, each treatment was repeated 4 times for a total of 52 cells each having an area of 15m²The effective component dosage is 8 g/mu (no application of medicine in the clear water control plot), and random block arrangement is adopted among plots.

According to the growth process of the cucumber, the control effects of different treatments on powdery mildew, cucumber downy mildew and cucumber anthracnose are investigated, and the specific statistical method is as follows:

the investigation method of the cucumber powdery mildew comprises the following steps: the disease condition of powdery mildew of cucumber is investigated on the field before the first pesticide application, the first pesticide application is carried out at the early stage of disease condition, the first and second pesticide applications are carried out after 7 days, the second pesticide application is carried out after 10 days, 4 points are randomly selected in each test cell, all leaves of 2 plants are investigated at each point, the classification record is carried out according to the percentage of the scab area on the leaves to the whole leaf area of the leaves, and the control effect is calculated according to the above formulas (formula 1 and formula 2).

Grading index of cucumber powdery mildew (leaf unit): level 0: no disease exists; level 1: the area of the lesion spots accounts for less than 5% of the whole area; and 3, level: the area of the lesion spots accounts for 6 to 10 percent of the whole area; and 5, stage: the area of the lesion spots accounts for 11 to 20 percent of the whole area; and 7, stage: the area of the lesion spots accounts for 21-40% of the whole area; and 9, stage: the area of the lesion spots accounts for more than 40 percent of the whole area.

The investigation method of the cucumber downy mildew comprises the following steps: the method comprises the steps of investigating the cucumber downy mildew incidence condition in the field before the first pesticide application, carrying out the first pesticide application at the early stage of the disease condition, carrying out the first investigation and the second pesticide application after 7 days, carrying out the second investigation 10 days after the second pesticide application, randomly selecting 4 points in each test cell, investigating all leaves of 2 plants at each point, recording in a grading manner according to the percentage of the scab area on each leaf to the whole leaf area of the leaf, and calculating the control effect according to the above formulas (formula 1 and formula 2).

Grade index of cucumber downy mildew (in leaves): stage 0: no disease; level 1: the area of the lesion spots accounts for less than 5% of the whole area; and 3, level: the area of the lesion spots accounts for 6 to 10 percent of the whole area; and 5, stage: the area of the lesion spots accounts for 11 to 25 percent of the whole area; and 7, stage: the area of the lesion spots accounts for 26 to 50 percent of the whole area; and 9, stage: the area of the lesion spots accounts for more than 50 percent of the whole area.

The investigation method of cucumber anthracnose comprises the following steps: the disease condition of cucumber anthracnose is investigated in field before the first pesticide application, the first pesticide application is carried out at the early stage of disease condition, the first investigation and the second pesticide application are carried out after 7 days, the second investigation is carried out after 10 days, 5-point sampling is adopted in each test cell, all leaves of 2 plants are investigated at each point, the total leaf number and the leaf number of each grade of disease are recorded in a grading manner according to the number of scabs on each leaf, the disease incidence of the leaves and the disease condition index are calculated, and the prevention and treatment effect is calculated according to the above formulas (formula 1 and formula 2).

Cucumber anthracnose disease grading index (in leaves): level 0: no lesion spots; level 1: 1-3 scabs are on a single leaf; and 3, level: 4-6 spots are formed on a single leaf; and 5, stage: 7-10 spots are on a single leaf; and 7, stage: 11-20 spots are formed on a single leaf, and the part is dense and flaky; and 9, stage: the lesion spots on a single leaf are dense and occupy more than 1/4 of the leaf area.

By comparing the most preferred examples with comparative examples 1 to 6, the Gowing method was used to evaluate the type of combined action of prothioconazole, pyraclostrobin and difenoconazole in the treatment of powdery mildew of cucumber, downy mildew of cucumber and anthracnose of cucumber.

Calculating the formula: cexp = C1+ C2 (1-C1), C1 and C2 are control effects when the bactericides A and B are used independently, cexp is a theoretical control effect of the bactericides A and B, and Cabs is an actual control effect after the bactericides A and B are mixed according to the proportion. Cabs > Cexp are synergistic, cabs < Cexp are antagonistic.

TABLE 5 prevention and treatment effects on cucumber diseases after treatment with different agents

TABLE 6 Combined action of prothioconazole, pyraclostrobin and difenoconazole on powdery mildew, cucumber downy mildew and cucumber anthracnose after 10 days of second drug combination

TABLE 7 Combined action of pyraclostrobin, prothioconazole and difenoconazole on powdery mildew of cucumber, downy mildew of cucumber and anthracnose of cucumber for 10 days after being combined with second drug

TABLE 8 Combined action of Difenoconazole with prothioconazole and pyraclostrobin on cucumber powdery mildew, cucumber downy mildew and cucumber anthracnose after 10d of second drug combination

As can be seen from the data of the test examples 1-6 and the comparative examples 1-6 in the tables 1 and 5, the control effects of the compound composition of prothioconazole, pyraclostrobin and difenoconazole on wheat powdery mildew, wheat scab, wheat leaf rust, cucumber powdery mildew, cucumber downy mildew and cucumber anthracnose are all more than 80 percent and are higher than those of a single-dose control agent and a binary compound agent. As can be seen from the data in tables 2, 3, 4, 6, 7 and 8, the actual control effects observed by the combinations containing prothioconazole, pyraclostrobin and difenoconazole in the preferable test example 1 are all greater than the expected control effects calculated according to the formula, which indicates that the combination of the three active ingredients has a synergistic effect compared with the binary compound preparation. And the adverse conditions such as phytotoxicity and the like do not appear in each treatment in the test, so that the prothioconazole, pyraclostrobin and difenoconazole compound composition disclosed by the invention has a synergistic interaction effect, can be used for preventing and treating various diseases of wheat in different growth cycles, reducing the application times, lowering the cost and reducing the pollution to the environment, and has the best prevention effect when the contents of prothioconazole, pyraclostrobin and difenoconazole are respectively 15%, 15% and 10%.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A pesticide composition is characterized in that the active ingredients of the pesticide composition contain prothioconazole, pyraclostrobin and difenoconazole; the weight ratio of prothioconazole, pyraclostrobin and difenoconazole in the pesticide composition is 3:3:2.

2. the pesticide composition as set forth in claim 1, wherein the weight percentage of prothioconazole in the pesticide composition is 0.1-40%;

and/or the pyraclostrobin accounts for 0.1-40% of the pesticide composition by weight;

and/or the weight percentage of the difenoconazole in the pesticide composition is 0.1-40%.

3. The pesticide composition as set forth in claim 2, wherein the weight percentage of prothioconazole in the pesticide composition is 1-30%;

and/or the pyraclostrobin accounts for 1-30% of the pesticide composition by weight;

and/or the weight percentage of the difenoconazole in the pesticide composition is 1-30%.

4. The pesticide composition as set forth in claim 2, wherein the weight percentage of prothioconazole in the pesticide composition is 10-20%;

and/or the pyraclostrobin accounts for 10-20% of the pesticide composition by weight;

and/or the weight percentage of the difenoconazole in the pesticide composition is 5-15%.

5. The pesticide composition as set forth in claim 1, wherein the content of the active ingredient in the pesticide composition is 1% to 95%.

6. The pesticide composition as set forth in claim 1, wherein the content of the active ingredient in the pesticide composition is 25% to 55%.

7. The pesticidal composition according to claim 1, characterized in that the content of the active ingredient in the pesticidal composition is 40%.

8. The pesticide composition as set forth in claim 5 further comprising an adjuvant.

9. The pesticide composition as set forth in claim 8, wherein the adjuvant is one or more of a wetting agent, a dispersant, an emulsifier, a solvent, a cosolvent, a thickener, a stabilizer, an antifreeze, an antifoaming agent, a preservative, a binder, a wall material, an acid adjusting medium, a base adjusting medium and a solid inert carrier.

10. The pesticide composition as set forth in claim 1, wherein the pesticide composition is in the form of one or more of a suspending agent, a seed treatment suspending agent, a suspoemulsion, a microcapsule suspending agent, an emulsion in water, a dispersible oil suspending agent, a wettable powder or a water dispersible granule.

11. Use of the pesticidal composition of any one of claims 1-10 for controlling one or more plant diseases of powdery mildew, head blight, leaf rust, downy mildew, anthracnose.

12. Use according to claim 11, wherein the plant comprises wheat, cucumber.

13. The use according to claim 11, wherein the pesticide composition is applied in an amount of 5 to 10 g/acre of active ingredient.

14. The use according to claim 11, wherein the pesticide composition is applied at an active ingredient level of 8 g/acre.