CN113243332B - 一种广泛脑区神经元树突发育障碍动物模型的制备与应用 - Google Patents
一种广泛脑区神经元树突发育障碍动物模型的制备与应用 Download PDFInfo
- Publication number
- CN113243332B CN113243332B CN202010082845.5A CN202010082845A CN113243332B CN 113243332 B CN113243332 B CN 113243332B CN 202010082845 A CN202010082845 A CN 202010082845A CN 113243332 B CN113243332 B CN 113243332B
- Authority
- CN
- China
- Prior art keywords
- neuronal
- tree
- sudden
- disorder
- brain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004556 brain Anatomy 0.000 title claims abstract description 84
- 238000010171 animal model Methods 0.000 title claims abstract description 40
- 210000002569 neuron Anatomy 0.000 title claims abstract description 40
- 238000009395 breeding Methods 0.000 title abstract description 6
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 230000001537 neural effect Effects 0.000 claims abstract description 66
- 206010021143 Hypoxia Diseases 0.000 claims abstract description 50
- 230000007954 hypoxia Effects 0.000 claims abstract description 42
- 208000037824 growth disorder Diseases 0.000 claims abstract description 24
- 238000012360 testing method Methods 0.000 claims abstract description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000001301 oxygen Substances 0.000 claims abstract description 19
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 19
- 230000007423 decrease Effects 0.000 claims abstract description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 16
- 238000012216 screening Methods 0.000 claims abstract description 8
- 210000001787 dendrite Anatomy 0.000 claims description 36
- 210000003520 dendritic spine Anatomy 0.000 claims description 34
- 230000002829 reductive effect Effects 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 30
- 150000001875 compounds Chemical class 0.000 claims description 15
- 208000035475 disorder Diseases 0.000 claims description 14
- 238000011161 development Methods 0.000 claims description 13
- 230000018109 developmental process Effects 0.000 claims description 13
- 238000009826 distribution Methods 0.000 claims description 13
- 230000024573 dendritic spine development Effects 0.000 claims description 10
- 206010012559 Developmental delay Diseases 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 9
- 230000001766 physiological effect Effects 0.000 claims description 9
- 230000009467 reduction Effects 0.000 claims description 9
- 229940124606 potential therapeutic agent Drugs 0.000 claims description 7
- 210000000225 synapse Anatomy 0.000 claims description 7
- 241000283984 Rodentia Species 0.000 claims description 4
- 230000009984 peri-natal effect Effects 0.000 claims description 4
- 241000282693 Cercopithecidae Species 0.000 claims description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 3
- 241000288906 Primates Species 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 208000010515 dystocia Diseases 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 12
- 229940079593 drug Drugs 0.000 abstract description 7
- 206010000210 abortion Diseases 0.000 abstract 1
- 231100000176 abortion Toxicity 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 27
- 230000006378 damage Effects 0.000 description 20
- 241000700159 Rattus Species 0.000 description 19
- 208000014674 injury Diseases 0.000 description 16
- 241000699670 Mus sp. Species 0.000 description 14
- 208000027418 Wounds and injury Diseases 0.000 description 14
- 230000000971 hippocampal effect Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 10
- 230000000946 synaptic effect Effects 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 7
- 210000001947 dentate gyrus Anatomy 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 230000001146 hypoxic effect Effects 0.000 description 7
- 230000006735 deficit Effects 0.000 description 6
- 230000003925 brain function Effects 0.000 description 5
- 229940124597 therapeutic agent Drugs 0.000 description 5
- 102000007469 Actins Human genes 0.000 description 4
- 108010085238 Actins Proteins 0.000 description 4
- 201000006474 Brain Ischemia Diseases 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 210000002503 granulosa cell Anatomy 0.000 description 4
- 210000001320 hippocampus Anatomy 0.000 description 4
- 230000000302 ischemic effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000002025 microglial effect Effects 0.000 description 4
- 210000000653 nervous system Anatomy 0.000 description 4
- 206010008120 Cerebral ischaemia Diseases 0.000 description 3
- 206010070511 Hypoxic-ischaemic encephalopathy Diseases 0.000 description 3
- 230000003542 behavioural effect Effects 0.000 description 3
- 230000004641 brain development Effects 0.000 description 3
- 206010008118 cerebral infarction Diseases 0.000 description 3
- 230000001149 cognitive effect Effects 0.000 description 3
- 230000002996 emotional effect Effects 0.000 description 3
- 210000004295 hippocampal neuron Anatomy 0.000 description 3
- 230000008506 pathogenesis Effects 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 230000000144 pharmacologic effect Effects 0.000 description 3
- 238000004904 shortening Methods 0.000 description 3
- 230000006886 spatial memory Effects 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 230000031836 visual learning Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000002381 Brain Hypoxia Diseases 0.000 description 2
- 208000012239 Developmental disease Diseases 0.000 description 2
- 102000004868 N-Methyl-D-Aspartate Receptors Human genes 0.000 description 2
- 108090001041 N-Methyl-D-Aspartate Receptors Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 210000005056 cell body Anatomy 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 208000010877 cognitive disease Diseases 0.000 description 2
- 230000003920 cognitive function Effects 0.000 description 2
- 230000003436 cytoskeletal effect Effects 0.000 description 2
- 230000002964 excitative effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000003193 general anesthetic agent Substances 0.000 description 2
- 230000009524 hypoxic brain injury Effects 0.000 description 2
- 230000001771 impaired effect Effects 0.000 description 2
- 239000011261 inert gas Substances 0.000 description 2
- 208000028867 ischemia Diseases 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 230000020796 long term synaptic depression Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000015654 memory Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 239000002547 new drug Substances 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 210000003538 post-synaptic density Anatomy 0.000 description 2
- 108010092804 postsynaptic density proteins Proteins 0.000 description 2
- 230000001242 postsynaptic effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 208000020016 psychiatric disease Diseases 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000008733 trauma Effects 0.000 description 2
- 210000003954 umbilical cord Anatomy 0.000 description 2
- 238000004804 winding Methods 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 208000007204 Brain death Diseases 0.000 description 1
- 208000014644 Brain disease Diseases 0.000 description 1
- 206010048964 Carotid artery occlusion Diseases 0.000 description 1
- 206010008089 Cerebral artery occlusion Diseases 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 102100037249 Egl nine homolog 1 Human genes 0.000 description 1
- 101710111663 Egl nine homolog 1 Proteins 0.000 description 1
- 206010016855 Foetal distress syndrome Diseases 0.000 description 1
- 102000018899 Glutamate Receptors Human genes 0.000 description 1
- 108010027915 Glutamate Receptors Proteins 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 238000012347 Morris Water Maze Methods 0.000 description 1
- 206010028923 Neonatal asphyxia Diseases 0.000 description 1
- 206010050081 Neonatal hypoxia Diseases 0.000 description 1
- 102000050267 Neurotensin Human genes 0.000 description 1
- 101800001814 Neurotensin Proteins 0.000 description 1
- 206010063837 Reperfusion injury Diseases 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 206010046797 Uterine ischaemia Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 108091006088 activator proteins Proteins 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 208000009973 brain hypoxia - ischemia Diseases 0.000 description 1
- 208000029028 brain injury Diseases 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 230000021617 central nervous system development Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000007278 cognition impairment Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000007831 electrophysiology Effects 0.000 description 1
- 238000002001 electrophysiology Methods 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 230000003492 excitotoxic effect Effects 0.000 description 1
- 231100000063 excitotoxicity Toxicity 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000004914 glial activation Effects 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 210000001879 hippocampal ca1 region Anatomy 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011173 large scale experimental method Methods 0.000 description 1
- 230000013016 learning Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000036997 mental performance Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000000274 microglia Anatomy 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 201000007309 middle cerebral artery infarction Diseases 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002052 molecular layer Substances 0.000 description 1
- 230000003990 molecular pathway Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000000626 neurodegenerative effect Effects 0.000 description 1
- 230000009251 neurologic dysfunction Effects 0.000 description 1
- 208000015015 neurological dysfunction Diseases 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 230000007658 neurological function Effects 0.000 description 1
- 230000008062 neuronal firing Effects 0.000 description 1
- 230000007121 neuropathological change Effects 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- PCJGZPGTCUMMOT-ISULXFBGSA-N neurotensin Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 PCJGZPGTCUMMOT-ISULXFBGSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 230000003956 synaptic plasticity Effects 0.000 description 1
- 230000005062 synaptic transmission Effects 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/35—Animals modified by environmental factors, e.g. temperature, O2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/106—Primate
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/107—Rabbit
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0306—Animal model for genetic diseases
- A01K2267/0318—Animal model for neurodegenerative disease, e.g. non- Alzheimer's
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Pathology (AREA)
- Rheumatology (AREA)
- Toxicology (AREA)
- Urology & Nephrology (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010082845.5A CN113243332B (zh) | 2020-02-07 | 2020-02-07 | 一种广泛脑区神经元树突发育障碍动物模型的制备与应用 |
| PCT/CN2021/075854 WO2021155866A1 (fr) | 2020-02-07 | 2021-02-07 | Préparation et application de modèle animal de dysplasie dendritique neuronale dans des régions cérébrales étendues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010082845.5A CN113243332B (zh) | 2020-02-07 | 2020-02-07 | 一种广泛脑区神经元树突发育障碍动物模型的制备与应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113243332A CN113243332A (zh) | 2021-08-13 |
| CN113243332B true CN113243332B (zh) | 2023-07-28 |
Family
ID=77200423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010082845.5A Active CN113243332B (zh) | 2020-02-07 | 2020-02-07 | 一种广泛脑区神经元树突发育障碍动物模型的制备与应用 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113243332B (fr) |
| WO (1) | WO2021155866A1 (fr) |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1521259A (zh) * | 2003-01-30 | 2004-08-18 | 清华大学 | 一种筛选抗痴呆药物的果蝇疾病模型及其构建方法与应用 |
| EP1929291A4 (fr) * | 2005-09-26 | 2010-12-22 | Univ California | Therapie par estriol pour maladie des troubles auto-immuns et neurodegeneratifs |
| US9388405B2 (en) * | 2009-02-18 | 2016-07-12 | Wayne State University | Method for identifying a subpopulation of mammalian cells with distinctive ribosome translation profiles |
| CN102037930A (zh) * | 2010-08-27 | 2011-05-04 | 浙江大学 | 新生儿持续性肺动脉高压动物模型的建立方法 |
| EP2653163A1 (fr) * | 2012-04-19 | 2013-10-23 | Université de Liège | Constituants estrogènes pour l'utilisation dans le traitement de troubles neurologiques |
| SI3024463T1 (sl) * | 2013-07-25 | 2020-08-31 | Neuren Pharmaceuticals Limited | Nevroprotektivne biciklične spojine in postopki za njihovo uporabo pri zdravljenju motenj avtističnega spektra in nevrorazvojnih motenj |
| CN103535322B (zh) * | 2013-09-17 | 2016-03-30 | 中国人民解放军成都军区总医院 | 一种高原脑水肿动物模型的构建方法 |
| CN107753957B (zh) * | 2016-08-23 | 2022-01-18 | 中国科学院上海药物研究所 | 一种非人哺乳动物中风或其相关疾病动物模型的建立方法及其用途 |
| EP3508207B1 (fr) * | 2016-08-30 | 2021-08-11 | Niigata University | Préparations cellulaires cultivées dans des conditions de faible teneur en oxygène et en sucre, et leurs applications en thérapie. |
-
2020
- 2020-02-07 CN CN202010082845.5A patent/CN113243332B/zh active Active
-
2021
- 2021-02-07 WO PCT/CN2021/075854 patent/WO2021155866A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| CN113243332A (zh) | 2021-08-13 |
| WO2021155866A1 (fr) | 2021-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bilimoria et al. | Microglia function during brain development: new insights from animal models | |
| Allan et al. | A mouse model of prenatal ethanol exposure using a voluntary drinking paradigm | |
| Fields | White matter in learning, cognition and psychiatric disorders | |
| Lillard et al. | Old dogs learning new tricks: Neuroplasticity beyond the juvenile period | |
| Ha et al. | Diabetes accelerates retinal ganglion cell dysfunction in mice lacking sigma receptor 1 | |
| Le et al. | Early retinoic acid deprivation in developing zebrafish results in microphthalmia | |
| Curtis et al. | An early-onset retinal dystrophy with dominant inheritance in the Abyssinian cat. Clinical and pathological findings. | |
| Datta et al. | Identification of cholinergic and non-cholinergic neurons in the pons expressing phosphorylated cyclic adenosine monophosphate response element-binding protein as a function of rapid eye movement sleep | |
| Royo et al. | Effects of n-3 polyunsaturated fatty acid supplementation on cognitive functions, electrocortical activity and neurogenesis in a non-human primate, the grey mouse lemur (Microcebus murinus) | |
| Shi et al. | Mimicking partial to total placental insufficiency in a rabbit model of cerebral palsy | |
| Trovato et al. | Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter | |
| Kabrita et al. | Development of the mouse suprachiasmatic nucleus: determination of time of cell origin and spatial arrangements within the nucleus | |
| Hoshino et al. | Ephrin‐A3 is required for tonotopic map precision and auditory functions in the mouse auditory brainstem | |
| Rao et al. | Elucidating mechanisms of neuronal circuit formation in layer 4 of the somatosensory cortex via intravital imaging | |
| CN113243332B (zh) | 一种广泛脑区神经元树突发育障碍动物模型的制备与应用 | |
| Tucker et al. | Sympathetic innervation alters growth and intrinsic heart rate of fetal rat atria maturing in oculo. | |
| Chen et al. | Differential activation of limbic circuitry associated with chronic ethanol withdrawal in DBA/2J and C57BL/6J mice | |
| Freedman et al. | Initial studies of embryonic transplants of human hippocampus and cerebral cortex derived from schizophrenic women | |
| Pappas et al. | Ectopic noradrenergic hyperinnervation does not functionally compensate for neonatal forebrain acetylcholine lesion | |
| Anegroaie et al. | Development of somatosensory‐evoked potentials in foetal sheep: effects of betamethasone | |
| Tang et al. | Transient sublethal hypoxia in neonatal rats causes reduced dendritic spines, aberrant synaptic plasticity, and impairments in memory | |
| Hegmann et al. | Gene differences influencing visual system function and behavior | |
| Saad et al. | The Effect of Retinoic Acid on the Development of Neural Tube at Early Stages of Chicken Embryo | |
| Stenstrom et al. | The Direction of response selectivity between conspecific and heterospecific auditory stimuli varies with response metric | |
| Cross | Postnatal Development of Neural Networks in the Healthy and Premature Brain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |