CN113237872A - Method for detecting pesticide residues of uncooked food - Google Patents
Method for detecting pesticide residues of uncooked food Download PDFInfo
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- CN113237872A CN113237872A CN202010556683.4A CN202010556683A CN113237872A CN 113237872 A CN113237872 A CN 113237872A CN 202010556683 A CN202010556683 A CN 202010556683A CN 113237872 A CN113237872 A CN 113237872A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Abstract
The invention discloses a method for detecting pesticide residues of uncooked food, and relates to the field of pesticide residue detection. Thermostated container, mounting box, draw bolt, color development test paper box and play liquid ware, a thermostated container surface is equipped with the spread groove, mounting box and spread groove welding, color development test paper box is installed inside the mounting box through drawing the bolt, it is connected through the screw thread screw cooperation with the thermostated container to go out the liquid ware. According to the invention, the liquid outlet device is arranged, the enzyme liquid in the test tube can be quickly sprayed out through the pressing plate, the pressing rod and the piston, the use is convenient and fast, and the activity of the enzyme is ensured through the arrangement of the constant temperature box; by using the thimerosal immobilized enzyme, the activity of the enzyme is better, and the detection of pesticide residues is facilitated; through setting up and drawing bolt, mounting box, can take out fast when needs color test paper.
Description
Technical Field
The invention belongs to the technical field of pesticide residue detection, and particularly relates to a method for detecting pesticide residues of uncooked food.
Background
The pesticide remains in agricultural production and uses the phenomenon that a part of pesticide directly or indirectly remains in cereal, vegetables, fruits, livestock products, aquatic products and soil and water after the pesticide, and if the pesticide residue is eaten by people or livestock, great danger will be caused, along with the improvement of people's living standard, very well view the problem of pesticide residue, and the requirement on the accuracy of detection device is higher and higher.
The existing pesticide residue detection device usually adopts a high performance liquid chromatography or an enzyme inhibition method, but the traditional enzyme inhibition method is not immobilized by thimerosal, has insufficient activity and has error risk in testing, so that the method for detecting pesticide residue of raw food is provided.
Disclosure of Invention
The invention aims to provide a method for detecting pesticide residues of uncooked food, and the problems that the traditional enzyme inhibition method is not immobilized by thimerosal, the activity is insufficient, and the test has error risk are solved.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a raw food pesticide residue detection device which comprises a constant temperature box, an installation box, a pulling bolt, a color development test paper box and a liquid outlet device, wherein a connecting groove is formed in one surface of the constant temperature box;
the mounting box includes mounting box body and first buffer spring, the color development test paper box includes color development test paper box body and second slider, the inside side surface of mounting box body is equipped with the second spout, color development test paper box passes through second slider, second spout sliding fit with the mounting box, first buffer spring one end and the inside lower surface welding of mounting box body, the first buffer spring other end and the lower surface welding of color development test paper box body, first buffer spring is in compression state at the beginning, and when the bolt no longer applys the resistance to color development test paper box, first buffer spring can cooperate the second slider to pop out the color development test paper box, conveniently takes out the color development test paper.
Preferably, the thermostated container includes thermostated container body, test tube, chamber door, fixed box, heat sensor, heater, the chamber door passes through hinged joint with the thermostated container body, a surface welding of chamber door has the handle, fixed box includes fixed box body, fixed plate and first slider, the fixed plate array has a plurality of mounting holes, and is a plurality of the test tube is connected with the mounting hole respectively, the inside bottom surface of thermostated container body is equipped with first spout, first slider and first spout sliding fit, the inside both sides face of thermostated container body is equipped with the mounting groove respectively, heat sensor and heater are connected with the mounting groove respectively, the test tube includes the drain tubule, and the thermostated container has guaranteed the vitality of enzyme, prevents that the enzyme from losing the detection function, through handle, first slider and spout, can quick replacement enzyme.
Preferably, the pull bolt comprises a pull bolt body, an elastic pin and a second buffer spring, through holes are respectively formed in the side faces of the mounting box and the color development test paper box in the same direction, two ends of the elastic pin are respectively connected with the through holes, and the second buffer spring is installed between the pull bolt body and the elastic pin.
Preferably, the liquid outlet device comprises a liquid outlet device body, a threaded hole, a pressing plate, a liquid outlet pipe, a nozzle and a reset spring, the threaded hole is precisely matched with the liquid guide thin pipe through a threaded screw hole, the liquid outlet device body is of a hollow pipe structure, one end of the liquid outlet pipe is connected with the inside of the liquid outlet device body, the other end of the liquid outlet pipe is connected with the nozzle, the pressing plate comprises a pressing plate body, a pressing rod and a piston, the piston is in sliding fit with the inner surface of the liquid outlet device body, one end of the pressing rod is welded with the lower surface of the pressing plate body, the other end of the pressing rod is welded with the upper surface of the piston, one end of the reset spring is welded with the lower surface of the piston, the other end of the reset spring is welded with the bottom surface inside of the liquid outlet device, the pressing plate is pushed downwards, the piston slides on the inner wall of the liquid outlet device to empty air in the liquid outlet device, when the bottom is reached, the pressing plate is loosened, the piston is pushed upwards by the reaction force of the reset spring, the enzyme liquid enters the spray head through the liquid outlet pipe.
Preferably, the heat sensor and the heater are connected to an external controller, the heating temperature of the heater is 0-40 ℃, the external controller comprises a display panel and a temperature adjusting knob, the external controller respectively controls the heat sensor and the heater, the display panel displays the temperature measured by the heat sensor, and the temperature adjusting knob can adjust the heating temperature of the heater according to different environments.
A detection method of a device for detecting pesticide residues of uncooked food comprises the following steps:
the method comprises the following steps: preparing a buffer solution and a thimerosal solution: putting disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate into a first reaction kettle, adding a hydrochloric acid solution to adjust the pH value of the solution to obtain a buffer solution, weighing thimerosal with a certain mass, putting the thimerosal into a second reaction kettle, and adding water to dissolve the thimerosal to obtain a thimerosal solution;
step two: preparing acetylcholinesterase (AChE) solution: dissolving electric eel acetylcholinesterase by using a buffer solution, putting the electric eel acetylcholinesterase into a third reaction kettle, and adding water to dilute the electric eel acetylcholinesterase to obtain an acetylcholinesterase solution;
step three: preparing a chitosan-acetylcholine esterase membrane and adding thimerosal: putting the chitosan membrane into acetylcholinesterase solution, adsorbing the chitosan membrane in a fourth reaction kettle, adding glutaraldehyde solution for reaction to prepare a chitosan-acetylcholinesterase membrane, and adding the prepared thimerosal solution in the reaction process to obtain detection solution;
step four: detection and analysis: spraying the detection liquid on the food to be detected, and testing by using a color development test paper.
Preferably, in the first step, disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate and water are slowly added into a first reaction kettle according to a ratio of 10: 1: 40, stirring is carried out while adding, the stirring time is 10min, hydrochloric acid is slowly poured into the first reaction kettle, a pH value in the reaction kettle is measured by a pH tester, the hydrochloric acid is stopped adding when the pH value reaches 7.5, a buffer solution is obtained, thimerosal and water are slowly added into a second reaction kettle according to a ratio of 1: 19, stirring is carried out while adding, and the stirring time is 30min, so that the thimerosal solution is obtained.
Preferably, in the second step, the buffer solution and water are slowly poured into a third reaction kettle according to the proportion of 1: 30, stirring is carried out while pouring to obtain a diluted solution, the electric eel acetylcholinesterase and water are slowly added into the third reaction kettle according to the proportion of 1.8: 1: 100 to obtain an acetylcholinesterase solution, and the acetylcholinesterase solution is stored at the temperature of minus 20 ℃.
Preferably, the chitosan membrane and the acetylcholinesterase solution prepared in the second step are added into a fourth reaction kettle, the standing time is 8-12 hours, the standing temperature is 2-4 ℃, glutaraldehyde solution with the concentration of 0.6-0.7% is added into the fourth reaction kettle, the reaction temperature is 4 ℃, the reaction time is 2-3 hours, detection liquid is obtained, and the detection liquid is placed into a test tube.
The invention has the following beneficial effects:
according to the invention, the liquid outlet device is arranged, the enzyme liquid in the test tube can be quickly sprayed out through the pressing plate, the pressing rod and the piston, the use is convenient and fast, and the activity of the enzyme is ensured through the arrangement of the constant temperature box;
the thimerosal immobilized enzyme is adopted, so that the activity of the enzyme is better, and the detection of pesticide residues is facilitated;
the invention can be quickly taken out when the color test paper is needed by arranging the pull plug and the mounting box.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of an uneaten food pesticide residue detection device of the present invention;
FIG. 2 is a schematic side view of the pesticide residue detection device for uncooked food of the present invention;
FIG. 3 is a schematic cross-sectional view taken along line A-A of FIG. 2 in accordance with the present invention;
FIG. 4 is a schematic cross-sectional view taken at C-C of FIG. 2 in accordance with the present invention;
FIG. 5 is a schematic view of the overall structure of the liquid outlet device of the present invention;
FIG. 6 is a schematic side view of the liquid outlet device of the present invention;
FIG. 7 is a schematic cross-sectional view taken along line A-A of FIG. 6 according to the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
100. a thermostat; 110. a test tube; 111. a liquid guide thin tube; 120. a box door; 121. a handle; 130. a fixed box; 131. a fixing plate; 132. a first slider; 140. a thermal sensor; 150. a heater; 200. mounting a box; 210. a first buffer spring; 300. pulling the bolt; 310. an elastic pin; 320. a second buffer spring; 400. a color development test paper box; 410. a second slider; 500. a liquid outlet device; 510. a threaded hole; 520. pressing a plate; 521. a pressure lever; 522. a piston; 530. a liquid outlet pipe; 540. a spray head; 550. a return spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "middle", "outer", "inner", and the like, indicate orientations or positional relationships, are used for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referenced components or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
The first embodiment is as follows:
referring to fig. 1-7, the present invention is a device for detecting pesticide residue in raw food. The color developing test paper box comprises a constant temperature box 100, a mounting box 200, a pulling bolt 300, a color developing test paper box 400 and a liquid outlet device 500, wherein a connecting groove is formed in one surface of the constant temperature box 100, the mounting box 200 is welded with the connecting groove, the color developing test paper box 400 is mounted inside the mounting box 200 through the pulling bolt 300, and the liquid outlet device 500 is connected with the constant temperature box 100 through a threaded screw hole in a matched mode;
the mounting box 200 includes mounting box body and first buffer spring 210, the color development test paper box 400 includes color development test paper box body and second slider 410, the inside side surface of mounting box body is equipped with the second spout, color development test paper box 400 passes through second slider 410 with mounting box 200, second spout sliding fit, first buffer spring 210 one end and the inside lower surface welding of mounting box body, the first buffer spring 210 other end and the lower surface welding of color development test paper box body, first buffer spring 210 is in compression state at the beginning, when the bolt 300 does not exert resistance to color development test paper box 400 any more, first buffer spring 210 can cooperate second slider 410 to pop out color development test paper box 400, conveniently take out the color development test paper.
Further, the incubator 100 includes an incubator body, test tubes 110, a door 120, a fixing box 130, a heat sensor 140, a heater 150, the door 120 is connected with the incubator body through a hinge, a handle 121 is welded on a surface of the door 120, the fixing box 130 includes a fixing box body, a fixing plate 131 and a first slider 132, the fixing plate 131 is provided with a plurality of mounting holes in an array manner, the test tubes 110 are respectively connected with the mounting holes, a first sliding groove is formed in the bottom surface inside the incubator body, the first slider 132 is in sliding fit with the first sliding groove, two side surfaces inside the incubator body are respectively provided with a mounting groove, the heat sensor 140 and the heater 150 are respectively connected with the mounting groove, the test tubes 110 include liquid guide tubules 111, the incubator 100 guarantees the activity of enzymes, the enzyme is prevented from losing a detection function, through the handle 121, the first slider 132 and the sliding grooves, the enzymes can be quickly replaced.
Further, the pull plug 300 includes a pull plug body, an elastic pin 310 and a second buffer spring 320, through holes are respectively formed in the side surfaces of the mounting box 200 and the color development test paper box 400 in the same direction, two ends of the elastic pin 310 are respectively connected with the through holes, and the second buffer spring 320 is installed between the pull plug body and the elastic pin 310.
Further, liquid outlet device 500 includes a liquid outlet device body, a threaded hole 510, a pressing plate 520, a liquid outlet pipe 530, a spray head 540 and a return spring 550, threaded hole 510 and liquid guiding thin tube 111 are precisely matched through a threaded screw hole, liquid outlet device body is a hollow tube structure, one end of liquid outlet pipe 530 is connected with the inside of liquid outlet device body, the other end of liquid outlet pipe 530 is connected with spray head 540, pressing plate 520 includes a pressing plate body, a pressing rod 521 and a piston 522, piston 522 is in sliding fit with the inner surface of liquid outlet device body, one end of pressing rod 521 is welded with the lower surface of pressing plate body, the other end of pressing rod 521 is welded with the upper surface of piston 522, one end of return spring 550 is welded with the lower surface of piston 522, the other end of return spring 550 is welded with the bottom surface of the inside of liquid outlet device 500, pressing plate 520 is pushed downwards, piston 522 slides on the inner wall of liquid outlet device 500 to evacuate air in liquid outlet device 500, when reaching the bottom, pressing plate 520 is loosened, the piston 522 is pushed upward by the reaction force of the return spring 550, and the enzyme solution enters the spray head 540 through the outlet pipe 530.
Further, the heat sensor 140 and the heater 150 are connected to an external controller, the heating temperature of the heater 150 is 0-40 ℃, the external controller includes a display panel and a temperature adjustment knob, the external controller controls the heat sensor 140 and the heater 150 respectively, wherein the model of the heater 150 is HVL031-300W, the model of the heat sensor 140 is EL-P202, the display panel displays the temperature measured by the heat sensor 140, and the temperature adjustment knob adjusts the heating temperature of the heater 150 according to different environments.
Example two:
a detection method of a device for detecting pesticide residues of uncooked food comprises the following steps:
the method comprises the following steps: preparing a buffer solution and a thimerosal solution: putting disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate into a first reaction kettle, adding a hydrochloric acid solution to adjust the pH value of the solution to obtain a buffer solution, weighing thimerosal with a certain mass, putting the thimerosal into a second reaction kettle, and adding water to dissolve the thimerosal to obtain a thimerosal solution;
step two: preparing acetylcholinesterase (AChE) solution: dissolving electric eel acetylcholinesterase by using a buffer solution, putting the electric eel acetylcholinesterase into a third reaction kettle, and adding water to dilute the electric eel acetylcholinesterase to obtain an acetylcholinesterase solution;
step three: preparing a chitosan-acetylcholine esterase membrane and adding thimerosal: putting the chitosan membrane into acetylcholinesterase solution, adsorbing the chitosan membrane in a fourth reaction kettle, adding glutaraldehyde solution for reaction to prepare a chitosan-acetylcholinesterase membrane, and adding the prepared thimerosal solution in the reaction process to obtain detection solution;
step four: detection and analysis: spraying the detection liquid on the food to be detected, and testing by using a color development test paper.
Further, in the first step, disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate and water are mixed according to a ratio of 10: 1: slowly adding the mixture into a first reaction kettle according to the proportion of 40, stirring while adding, wherein the stirring time is 10min, slowly pouring hydrochloric acid into the first reaction kettle, measuring the pH value in the reaction kettle by using a pH tester, stopping adding the hydrochloric acid when the pH value reaches 7.5 to obtain a buffer solution, slowly adding the thimerosal and water into a second reaction kettle according to the proportion of 1: 19, stirring while adding, and stirring for 30min to obtain the thimerosal solution.
Further, in the second step, the buffer solution and water are slowly poured into a third reaction kettle according to the proportion of 1: 30, stirring is carried out while pouring to obtain a diluted solution, the electric eel acetylcholinesterase and water are slowly added into the third reaction kettle according to the proportion of 1.8: 1: 100 to obtain an acetylcholinesterase solution, and the acetylcholinesterase solution is stored at the temperature of minus 20 ℃.
Further, the chitosan membrane and the acetylcholinesterase solution prepared in the second step are added into a fourth reaction kettle, the standing time is 8-12 hours, the standing temperature is 2-4 ℃, glutaraldehyde solution with the concentration of 0.6-0.7% is added into the fourth reaction kettle, the reaction temperature is 4 ℃, the reaction time is 2-3 hours, detection liquid is obtained, and the detection liquid is placed into the test tube 110.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (9)
1. The utility model provides a incomplete detection device of uncooked food farming, includes thermostated container (100), mounting box (200), draws bolt (300), colour development test paper box (400) and goes out liquid ware (500), its characterized in that: one surface of the incubator (100) is provided with a connecting groove, the mounting box (200) is welded with the connecting groove, the color development test paper box (400) is mounted inside the mounting box (200) through a pull bolt (300), and the liquid outlet device (500) is connected with the incubator (100) in a matched manner through a threaded screw hole;
mounting box (200) are including mounting box body and first buffer spring (210), color development test paper box (400) are including color development test paper box body and second slider (410), the inside side surface of mounting box body is equipped with the second spout, color development test paper box (400) and mounting box (200) are through second slider (410), second spout sliding fit, first buffer spring (210) one end and the inside lower surface welding of mounting box body, first buffer spring (210) other end and color development test paper box body lower surface welding.
2. The uneaten food pesticide residue detection device of claim 1, characterized in that: thermostat (100) include thermostat body, test tube (110), chamber door (120), fixed case (130), heat sensor (140), heater (150), hinged joint is passed through with the thermostat body in chamber door (120), a surface welding of chamber door (120) has handle (121), fixed case (130) are including fixed case body, fixed plate (131) and first slider (132), fixed plate (131) array has a plurality of mounting holes, and is a plurality of test tube (110) are connected with the mounting hole respectively, the inside bottom surface of thermostat body is equipped with first spout, first slider (132) and first spout sliding fit, the inside both sides face of thermostat body is equipped with the mounting groove respectively, heat sensor (140) are connected with the mounting groove respectively with heater (150), test tube (110) include drain tubule (111).
3. The uneaten food pesticide residue detection device of claim 1, characterized in that: the pull bolt (300) comprises a pull bolt body, an elastic pin (310) and a second buffer spring (320), through holes are respectively formed in the side faces of the mounting box (200) and the color development test paper box (400) in the same direction, two ends of the elastic pin (310) are respectively connected with the through holes, and the second buffer spring (320) is mounted between the pull bolt body and the elastic pin (310).
4. The uncooked food pesticide residue detection device according to claim 1 or 2, wherein: the liquid outlet device (500) comprises a liquid outlet device body, a threaded hole (510), a pressure plate (520), a liquid outlet pipe (530), a spray head (540) and a return spring (550), the threaded hole (510) is precisely matched with the liquid guide thin tube (111) through a threaded screw hole, the liquid outlet device body is of a hollow tube structure, one end of the liquid outlet tube (530) is connected with the inside of the liquid outlet device body, the other end of the liquid outlet pipe (530) is connected with a spray head (540), the pressure plate (520) comprises a pressure plate body, a pressure rod (521) and a piston (522), the piston (522) is in sliding fit with the inner surface of the liquid outlet device body, one end of the pressure lever (521) is welded with the lower surface of the pressure plate body, the other end of the pressure lever (521) is welded with the upper surface of the piston (522), one end of the return spring (550) is welded with the lower surface of the piston (522), the other end of the return spring (550) is welded with the bottom surface inside the liquid outlet device (500).
5. The device for detecting pesticide residues on uncooked food according to any one of claims 1 to 4, wherein: the heat sensor (140) and the heater (150) are connected with an external controller, the heating temperature of the heater (150) is 0-40 ℃, and the external controller comprises a display panel and a temperature adjusting knob.
6. The detection method of the device for detecting pesticide residues of uncooked food as claimed in any one of claims 1-5, wherein: the method comprises the following steps:
the method comprises the following steps: preparing a buffer solution and a thimerosal solution: putting disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate into a first reaction kettle, adding a hydrochloric acid solution to adjust the pH value of the solution to obtain a buffer solution, weighing thimerosal with a certain mass, putting the thimerosal into a second reaction kettle, and adding water to dissolve the thimerosal to obtain a thimerosal solution;
step two: preparing acetylcholinesterase (AChE) solution: dissolving electric eel acetylcholinesterase by using a buffer solution, putting the electric eel acetylcholinesterase into a third reaction kettle, and adding water to dilute the electric eel acetylcholinesterase to obtain an acetylcholinesterase solution;
step three: preparing a chitosan-acetylcholine esterase membrane and adding thimerosal: putting the chitosan membrane into acetylcholinesterase solution, adsorbing the chitosan membrane in a fourth reaction kettle, adding glutaraldehyde solution for reaction to prepare a chitosan-acetylcholinesterase membrane, and adding the prepared thimerosal solution in the reaction process to obtain detection solution;
step four: detection and analysis: spraying the detection liquid on the food to be detected, and testing by using a color development test paper.
7. The detection method of the uncooked food pesticide residue detection device according to claim 6, wherein the detection method comprises the following steps: in the first step, disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate and water are slowly added into a first reaction kettle according to the proportion of 10: 1: 40, stirring is carried out while adding, the stirring time is 10min, hydrochloric acid is slowly poured into the first reaction kettle, the pH value in the reaction kettle is measured by a pH tester, the hydrochloric acid is stopped adding when the pH value reaches 7.5, a buffer solution is obtained, thimerosal and water are slowly added into a second reaction kettle according to the proportion of 1: 19, stirring is carried out while adding, and the stirring time is 30min, so that the thimerosal solution is obtained.
8. The detection method for the uncooked food pesticide residue detection device according to claim 6, wherein the detection method comprises the following steps: and in the second step, the buffer solution and water are slowly poured into a third reaction kettle according to the proportion of 1: 30, stirring is carried out while chamfering to obtain a diluted solution, the electric eel acetylcholinesterase and water are slowly added into the third reaction kettle according to the proportion of 1.8: 1: 100 to obtain an acetylcholinesterase solution, and the acetylcholinesterase solution is stored at the temperature of-20 ℃.
9. The detection method of the device for detecting pesticide residues of uncooked food according to any one of claims 6 to 8, comprising the following steps: and (3) adding the chitosan membrane and the acetylcholinesterase solution prepared in the second step into a fourth reaction kettle, standing for 8-12 hours at the temperature of 2-4 ℃, adding a glutaraldehyde solution with the concentration of 0.6-0.7% into the fourth reaction kettle, reacting at the temperature of 4 ℃ for 2-3 hours, and thus obtaining a detection solution.
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| CN210338933U (en) * | 2019-08-01 | 2020-04-17 | 北京纳百生物科技有限公司 | Vitamin rapid detection kit |
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| CN207408227U (en) * | 2017-10-25 | 2018-05-25 | 苏州国奥印刷科技有限公司 | A kind of carton cavity resistance to compression detection device |
| CN207923896U (en) * | 2018-03-27 | 2018-09-28 | 山西医科大学第二医院 | Portable division of endocrinology's blood sugar detection apparatus |
| CN209417055U (en) * | 2018-12-29 | 2019-09-20 | 上海怡豪生物科技有限公司 | A kind of kit for CAR-T cell factor ELISA detection |
| CN209889508U (en) * | 2019-04-22 | 2020-01-03 | 桐乡市第一人民医院 | Convenient test paper box |
| CN109883964A (en) * | 2019-04-24 | 2019-06-14 | 宁夏金世纪包装印刷有限公司 | The anti-detection device of box or case mould |
| CN210338933U (en) * | 2019-08-01 | 2020-04-17 | 北京纳百生物科技有限公司 | Vitamin rapid detection kit |
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