CN113230200A - Ointment for treating bacterial skin diseases and preparation method thereof - Google Patents
Ointment for treating bacterial skin diseases and preparation method thereof Download PDFInfo
- Publication number
- CN113230200A CN113230200A CN202110493167.6A CN202110493167A CN113230200A CN 113230200 A CN113230200 A CN 113230200A CN 202110493167 A CN202110493167 A CN 202110493167A CN 113230200 A CN113230200 A CN 113230200A
- Authority
- CN
- China
- Prior art keywords
- parts
- ointment
- skin diseases
- component
- treating bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/30—Zinc; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/14—Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Abstract
The invention discloses an ointment for treating bacterial skin diseases and a preparation method thereof, wherein zinc glycolate, urea, glyceryl stearate, triethanolamine and carbomer are added with a proper amount of water to be heated and ground until the mixture is pasty to form a component A; taking cetearyl alcohol, parabens or gallate compounds, grinding in a mortar, and dissolving with ethanol until the mixture is pasty to form a component B; heating component A to appropriate temperature, slowly adding essence and component B into component A, stirring while adding in one direction, mixing, stirring, and cooling to room temperature to obtain the final product. The ointment prepared by the invention is mainly used for treating bacterial skin diseases, and can also be used for treating skin diseases such as dermatitis, eczema and the like caused by bacteria.
Description
Technical Field
The invention belongs to the technical field of medicines and preparation thereof, and particularly relates to an ointment for treating bacterial skin diseases and a preparation method thereof.
Background
The bacterial skin diseases refer to skin lesions caused by bacterial infection and bacterial toxin invasion, the skin lesions are common at exposed parts such as heads, faces, necks and hands, clinically show red, swollen, hot, painful and purulent parts, have certain transmissibility, and the transmission route is contact transmission, the main pathogenesis of the bacterial skin diseases is allergic reaction (allergic to bacterial products) or toxic reaction (damage caused by bacterial toxins), and the bacterial skin diseases can be divided into acute and chronic diseases, most of the bacterial skin diseases, especially the coccal skin diseases have acute attack and short course of disease; only a few of the chronic diseases, such as skin tuberculosis, leprosy and the like, have the treatment principles of diminishing inflammation, sterilizing, drying and astringing, and preventing the inflammation from spreading.
Zinc glycolate is an organic zinc-containing compound with an antibacterial effect, the main bactericidal active component is divalent zinc ions, and due to the oxidation-reduction property of the zinc ions, when the zinc ions are combined with bacterial cell membranes, the zinc glycolate reacts with organic matters in the bacterial cell membranes to destroy the structure of membrane proteins and lose the activity of the membrane proteins, so that the aim of sterilization is fulfilled.
Urea is often used as an ingredient of an ointment to increase the moisture of the skin, and at the same time, urea also dissolves proteins, increases the water content of the stratum corneum, increases the hydration of the stratum corneum, makes the skin soft, and has antibacterial, antipruritic, and granulation-promoting effects.
Methyl paraben is commonly used as a sterilization preservative for organic synthesis, food, cosmetics and medicines, and has a phenolic hydroxyl structure and strong antibacterial performance, and the antibacterial action mechanism is as follows: destroying the cell membrane of the microorganism, denaturing the protein in the cell, and inhibiting the activities of the respiring enzyme system and the electron transfer enzyme system of the microorganism cell.
The invention aims to achieve good antibacterial treatment effect through the synergistic and addition effects generated by the combination of zinc glycolate serving as a main drug and methyl p-hydroxybenzoate serving as an auxiliary material and an antibacterial compound, and simultaneously reduce the irritation effect on skin caused by long-term administration.
Disclosure of Invention
The invention provides a novel ointment and a preparation method thereof, aiming at overcoming the defects of the existing ointment for treating bacterial skin diseases, and aiming at preparing a compound ointment by combining a plurality of antibacterial agents so as to improve the healing effect, shorten the healing time and reduce the irritation to the skin.
The technical scheme adopted by the invention is as follows:
a preparation method of an ointment for treating bacterial skin diseases comprises the following steps:
s1: heating zinc glycolate, urea, glyceryl stearate, triethanolamine and carbomer with appropriate amount of water, and grinding until paste is formed to form component A;
s2: taking cetearyl alcohol, parabens or gallate compounds, grinding in a mortar, and dissolving with ethanol until the mixture is pasty to form a component B;
s3: heating component A to appropriate temperature, slowly adding essence and component B into component A, stirring while adding in one direction, mixing, stirring, and cooling to room temperature to obtain the final product.
Furthermore, the paraben compound is selected from methyl paraben, ethyl paraben and propyl paraben, and the gallic acid ester is selected from methyl gallate, ethyl gallate and propyl gallate.
The preparation method comprises the following steps of: 100-200 parts of zinc glycolate, 30-70 parts of glycerol, 40-60 parts of cetostearyl alcohol, 100-150 parts of urea, 80-120 parts of glyceryl stearate, 30-70 parts of triethanolamine, 30-70 parts of carbomer, 60-100 parts of p-hydroxybenzoate or gallate compound, 80-120 parts of ethanol and 10-50 parts of essence.
Further, the feed additive is prepared from the following components in parts by weight: 150 parts of zinc glycolate, 50 parts of glycerol, 50 parts of cetostearyl alcohol, 120 parts of urea, 100 parts of glyceryl stearate, 40 parts of triethanolamine, 40 parts of carbomer, 80 parts of methyl p-hydroxybenzoate, 80 parts of ethanol and 20 parts of essence.
Further, in step S1, the amount of water is 100-150 parts, the heating temperature is 70-80 ℃, the heat preservation time is 30-60 minutes, and the grinding time is 40-60 minutes.
Further, in step S2, the amount of ethanol used is 60 to 100 parts, and the grinding time is 40 to 60 minutes.
Further, in step S3, the stirring time is 20 to 30 minutes.
Another technical scheme adopted by the invention is the ointment obtained by the method.
The invention aims to achieve the synergy and addition effect by utilizing the combined use of materials such as zinc glycolate, methylparaben and the like, thereby achieving good treatment effect. The ointment prepared by the invention is mainly used for treating bacterial skin diseases, and can also be used for treating skin diseases such as dermatitis, eczema and the like caused by bacteria.
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto:
it is to be understood that these examples are preferred embodiments of the invention and are intended only for purposes of illustration. From the above discussion and these examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and technical scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions.
Example 1 drug effect test for curing bacterial skin diseases
The method takes zinc glycolate with a single component, methyl p-hydroxybenzoate with a single component and the ointment prepared by the invention to carry out a mouse test, so as to investigate the treatment effect of the ointment through the variable quantity of the conventional index of blood in the same time, and comprises the following specific operation steps:
(1) selecting a Balb/c female nude mouse with SPF grade of 5 weeks as a verification object, carrying out staphylococcus aureus infection on the nude mouse to enable the nude mouse to suffer from bacterial skin diseases, and detecting the conventional blood indexes of the nude mouse;
(2) the zinc glycolate, the methylparaben and the ointment prepared by the invention with the same dosage are used for treating the mice with similar disease degrees, and the treatment effect of various medicines is judged by investigating the conventional blood indexes of the mice, such as white blood cell count, lymphocyte count and the like;
(3) the treatment effect of various medicines is judged by judging the change amplitude of the conventional blood index of the mouse in the same time, and the specific experimental data are shown in tables 1, 2 and 3:
TABLE 1 influence of Zinc glycolate on the blood indices of mice with bacterial skin diseases
TABLE 2 influence degree of methylparaben on the blood index of mice with bacterial skin diseases
TABLE 3 influence degree of ointment prepared by the present invention on the conventional index of blood of mice with bacterial skin diseases
Note: the blood general index reference range of healthy SPF grade Balb/c mice:
white blood cell count: 2.5-6X 109Per liter; lymphocyte count: 1.75-5.20X 109Per L
As for example 1, by examining the conventional index of blood of a mouse, it can be seen that, for bacterial skin diseases, the ointment prepared by the invention needs 11 days and 9 days to respectively restore the white blood cell count and the lymphocyte count to the reference range of the conventional index of blood of the mouse, and the white blood cell count and the lymphocyte count are respectively reduced by 12 days and 12 days compared with the zinc glycolate with a single component; compared with methyl p-hydroxybenzoate of single component, the amount of the active component is reduced by 16 days and 18 days respectively. The time required for curing is shorter, and the ointment prepared by the invention has good treatment effect on bacterial skin diseases and has certain cooperativity and additive property.
Example 2 drug effect test on eczema healing
The method takes zinc glycolate with a single component, methyl p-hydroxybenzoate with a single component and the ointment prepared by the invention to carry out a mouse test, so as to investigate the treatment effect of the ointment through the variable quantity of the conventional index of blood in the same time, and comprises the following specific operation steps:
(1) selecting a Balb/c female nude mouse with SPF grade of 5 weeks as a verification object to cause the nude mouse to suffer from eczema;
(2) the zinc glycolate, the methylparaben and the ointment prepared by the invention with the same dosage are used for treating the mice with similar disease degrees, and the treatment effect of various medicines is judged by investigating the conventional blood indexes of the mice, such as white blood cell count, lymphocyte count and the like;
(3) the treatment effect of various medicines is judged by judging the change amplitude of the conventional index of the blood of the mouse in the same time, and the specific experimental data are shown in tables 4, 5 and 6:
TABLE 4 influence of Zinc glycolate on the blood indices of mice with eczema
TABLE 5 degree of influence of methylparaben on the blood indices of mice with eczema
TABLE 6 influence degree of ointment prepared by the present invention on blood routine index of mice with eczema
Note: the blood general index reference range of healthy SPF grade Balb/c mice:
white blood cell count: 2.5-6X 109Per liter; lymphocyte count: 1.75-5.20X 109Per L
For example 2, by examining the conventional blood index of the mouse, it can be seen that, for the mouse with eczema, the ointment prepared by the invention needs 11 days and 9 days to respectively recover the white blood cell count and the lymphocyte count to the reference range of the conventional blood index of the mouse, and the white blood cell count and the lymphocyte count are reduced by 14 days and 18 days compared with the single component of zinc glycolate; the reduction was 16 days, 18 days compared to the single component methyl paraben. The time required for curing is shorter, which obviously shows that the ointment prepared by the invention has good treatment effect on eczema and has certain cooperativity and additive property.
EXAMPLE 3 study of irritation of drug on mouse skin
The mouse skin irritation experiment is carried out by taking zinc glycolate with a single component, methyl p-hydroxybenzoate with a single component, the ointment prepared by the invention and normal saline, and the specific operation steps are as follows:
(1) selecting 40 Balb/c nude mice with SPF grade and age of 5 weeks as verification objects, and 20 mice each in male and female;
(2) 40 mice with intact skin were divided equally into four groups: a single component zinc hydroxyacetate component (A component), a single component methyl p-hydroxybenzoate component (B component), a paste component (C component) prepared by the invention and a normal saline component (D component);
(3) the mice were depilated 24h prior to the experiment by first shearing the back and sides and depilating with depilatory cream, approximately 3cm x 3cm on each side. Adopting a same-body left-right comparison method, coating the tested medicine on the hair removal area on the left side, and taking the right side as a control;
(4) the zinc glycolate with a single component, the methyl p-hydroxybenzoate with a single component, the ointment prepared by the invention and the physiological saline are acted on the tested part of the mouse under the same condition;
(5) each time lasts for 0.5h, after 0.5h, the medicine is washed out by warm water, once a day, the medicine is applied for one week, the observation is continuously carried out for 7 days, and the skin irritation scores of the mice are respectively observed on 1 day, 3 days and 7 days.
(6) The results are shown in Table 7, the skin irritation response score criteria are shown in Table 8, and the skin irritation intensity evaluation criteria are shown in Table 9.
TABLE 7 results of repeated administration of the skin irritation test in mice
TABLE 8 mouse skin irritation response Scoring criteria
TABLE 9 mouse skin irritation Strength Scoring Standard
| Mean score | Evaluation of | Mean score | Evaluation of |
| 0～0.49 | Has no irritation | 3～5.99 | Moderate irritation |
| 0.5～2.99 | Mild irritation | 6～8 | Severe irritation |
As can be seen from the results of example 3, the ointment prepared according to the present invention (average stimulation response score: 1.6, which is mild irritation) has lower skin irritation and better therapeutic effect than the single component zinc glycolate (average stimulation response score: 3.8, which is moderate irritation) and the single component methylparaben (average stimulation response score: 4.2, which is moderate irritation).
In conclusion, the bacterial ointment and the preparation method thereof have certain treatment effect on bacterial skin diseases and eczema, and compared with the antibacterial single component of zinc glycolate and the antibacterial single component of methyl p-hydroxybenzoate, the antibacterial ointment prepared by the invention has the advantages of shorter treatment time, lower skin irritation, stronger antibacterial performance and obvious synergy.
The above-described embodiments are merely exemplary embodiments of the present invention, which should not be construed as limiting the invention in any way, and any modifications, substitutions, and improvements made on the basis of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A preparation method of an ointment for treating bacterial skin diseases is characterized by comprising the following steps: the method comprises the following steps:
s1: heating zinc glycolate, urea, glyceryl stearate, triethanolamine and carbomer with appropriate amount of water, and grinding until paste is formed to form component A;
s2: taking cetearyl alcohol, parabens or gallate compounds, grinding in a mortar, and dissolving with ethanol until the mixture is pasty to form a component B;
s3: heating component A to appropriate temperature, slowly adding essence and component B into component A, stirring while adding in one direction, mixing, stirring, and cooling to room temperature to obtain the final product.
2. The method for preparing an ointment for treating bacterial skin diseases according to claim 1, wherein the ointment comprises the following components: the paraben compound is selected from methyl paraben, ethyl paraben and propyl paraben, and the gallic acid ester is selected from methyl gallate, ethyl gallate and propyl gallate.
3. The method for preparing an ointment for treating bacterial skin diseases according to claim 1, wherein the ointment comprises the following components: the composition is prepared from the following components in parts by weight: 100-200 parts of zinc glycolate, 30-70 parts of glycerol, 40-60 parts of cetostearyl alcohol, 100-150 parts of urea, 80-120 parts of glyceryl stearate, 30-70 parts of triethanolamine, 30-70 parts of carbomer, 60-100 parts of p-hydroxybenzoate or gallate compound, 80-120 parts of ethanol and 10-50 parts of essence.
4. The method for preparing an ointment for treating bacterial skin diseases according to claim 3, wherein the ointment comprises the following components: the composition is prepared from the following components in parts by weight: 150 parts of zinc glycolate, 50 parts of glycerol, 50 parts of cetostearyl alcohol, 120 parts of urea, 100 parts of glyceryl stearate, 40 parts of triethanolamine, 40 parts of carbomer, 80 parts of methyl p-hydroxybenzoate, 80 parts of ethanol and 20 parts of essence.
5. The method for preparing an ointment for treating bacterial skin diseases according to claim 1, wherein the ointment comprises the following components: in the step S1, the using amount of water is 100-150 parts, the heating temperature is 70-80 ℃, the heat preservation time is 30-60 minutes, and the grinding time is 40-60 minutes.
6. The method for preparing an ointment for treating bacterial skin diseases according to claim 1, wherein the ointment comprises the following components: in step S2, the amount of ethanol is 60-100 parts, and the grinding time is 40-60 minutes.
7. The method for preparing an ointment for treating bacterial skin diseases according to claim 1, wherein the ointment comprises the following components: in step S3, the stirring time is 20-30 minutes.
8. An ointment for treating bacterial skin diseases, prepared by the preparation method of any one of claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110493167.6A CN113230200A (en) | 2021-05-07 | 2021-05-07 | Ointment for treating bacterial skin diseases and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110493167.6A CN113230200A (en) | 2021-05-07 | 2021-05-07 | Ointment for treating bacterial skin diseases and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113230200A true CN113230200A (en) | 2021-08-10 |
Family
ID=77132174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110493167.6A Pending CN113230200A (en) | 2021-05-07 | 2021-05-07 | Ointment for treating bacterial skin diseases and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113230200A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113274428A (en) * | 2021-05-11 | 2021-08-20 | 浙江工业大学 | Ointment for treating acute pruritic skin diseases and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1080526A (en) * | 1992-03-13 | 1994-01-12 | 大塚制药株式会社 | Infect the Therapeutic Method of disease, the anti-corrosion method of cosmetics, antibiotic and antimycotic agent and cosmetics |
| CN1337873A (en) * | 1999-01-28 | 2002-02-27 | 株式会社资生堂 | Compositions for external use |
| CN1513458A (en) * | 2002-12-31 | 2004-07-21 | 北京优力凯生物技术有限责任公司 | Compound silver zinc sulfapyrimidine ointment, and its prepn. method |
| CN101163455A (en) * | 2005-01-07 | 2008-04-16 | 纽约市哥伦比亚大学托管会 | Non-irritating compositions containing zinc salts |
| CN101590077A (en) * | 2009-06-03 | 2009-12-02 | 南京长澳医药科技有限公司 | A kind of compound erythromycin zinc acetate ointment for local use |
| CN110035762A (en) * | 2016-12-09 | 2019-07-19 | 杰富意矿物股份有限公司 | The excellent inorganic compositions of zinc ion slow release and its manufacturing method |
-
2021
- 2021-05-07 CN CN202110493167.6A patent/CN113230200A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1080526A (en) * | 1992-03-13 | 1994-01-12 | 大塚制药株式会社 | Infect the Therapeutic Method of disease, the anti-corrosion method of cosmetics, antibiotic and antimycotic agent and cosmetics |
| CN1337873A (en) * | 1999-01-28 | 2002-02-27 | 株式会社资生堂 | Compositions for external use |
| CN1513458A (en) * | 2002-12-31 | 2004-07-21 | 北京优力凯生物技术有限责任公司 | Compound silver zinc sulfapyrimidine ointment, and its prepn. method |
| CN101163455A (en) * | 2005-01-07 | 2008-04-16 | 纽约市哥伦比亚大学托管会 | Non-irritating compositions containing zinc salts |
| CN101590077A (en) * | 2009-06-03 | 2009-12-02 | 南京长澳医药科技有限公司 | A kind of compound erythromycin zinc acetate ointment for local use |
| CN110035762A (en) * | 2016-12-09 | 2019-07-19 | 杰富意矿物股份有限公司 | The excellent inorganic compositions of zinc ion slow release and its manufacturing method |
Non-Patent Citations (3)
| Title |
|---|
| 丁浩、童忠良、杜高翔: "《纳米抗菌技术》", 31 January 2008, 化学工业出版社 * |
| 罗明生: "《现代临床药物大典》", 31 January 2004, 四川科学技术出版社 * |
| 黄峻、黄祖瑚: "《临床药物手册》", 31 January 2015, 上海科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113274428A (en) * | 2021-05-11 | 2021-08-20 | 浙江工业大学 | Ointment for treating acute pruritic skin diseases and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110585114B (en) | Bactericidal composition for regulating skin microbial flora and application thereof | |
| US8481049B2 (en) | Methods for treating a wound using epidermal growth factor formulation | |
| CN108136026A (en) | The composition based on vaseline comprising cationic biocides | |
| CN109431873B (en) | Composition for repairing and relieving skin inflammation | |
| JP2000504740A (en) | Cosmetic, dermatological or veterinary composition for aseptic treatment of human and animal skin | |
| JP2003515615A (en) | Antimicrobial composition for treatment | |
| US20140271881A1 (en) | Antiseptic composition | |
| CN111671692A (en) | Acne-removing composition and preparation method and application thereof | |
| CN115569105A (en) | Composition containing rice hull extract for removing dandruff, controlling oil, inhibiting bacteria and removing mites | |
| CN113230200A (en) | Ointment for treating bacterial skin diseases and preparation method thereof | |
| CN101129409A (en) | Antimycotic externally used drug | |
| KR101835799B1 (en) | A cosmetic composition for manufacturing by the using of propolis, and the cosmetic manufactured by the composition | |
| CN113456735B (en) | Inactivated probiotic herbal gel and preparation method and application thereof | |
| WO2019123483A1 (en) | A herbal antiseptic and wound healing preparation | |
| HU186767B (en) | Process for producing compositions for treating dermatologicalanomalys | |
| RU2708069C1 (en) | Agent for treating mastitis in cows | |
| CN106729673A (en) | A kind of composition for the superficial surface of a wound | |
| EP3638219B1 (en) | Composition comprising terpinene-4-ol for the treatment of demodicosis | |
| AU2012250489B2 (en) | Avian-based treatment for microbial infections | |
| EP3313369B1 (en) | Emollient composition | |
| CN115487226B (en) | External natural bactericide pharmaceutical composition | |
| CN110585478A (en) | Hair-planting restoration liquid and preparation method thereof | |
| RU2785542C2 (en) | Composition for complex skin care in the treatment of dermatosis | |
| US11666622B2 (en) | Topical treatment for animals | |
| RU2745236C1 (en) | Method of treatment and prevention of subclinical mastitis of cows |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210810 |