CN113229156A - Animal hypoxia-sensitive individual screening device - Google Patents
Animal hypoxia-sensitive individual screening device Download PDFInfo
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- CN113229156A CN113229156A CN202110502333.4A CN202110502333A CN113229156A CN 113229156 A CN113229156 A CN 113229156A CN 202110502333 A CN202110502333 A CN 202110502333A CN 113229156 A CN113229156 A CN 113229156A
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- container
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- carbon dioxide
- animal
- screening device
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- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 46
- 230000007954 hypoxia Effects 0.000 title claims abstract description 44
- 241001465754 Metazoa Species 0.000 title claims abstract description 35
- 238000012216 screening Methods 0.000 title claims abstract description 34
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 64
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 36
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 35
- 239000001301 oxygen Substances 0.000 claims abstract description 35
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 23
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 238000012545 processing Methods 0.000 claims abstract description 6
- 239000004677 Nylon Substances 0.000 claims description 3
- 230000005489 elastic deformation Effects 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 241000700159 Rattus Species 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 9
- 206010048962 Brain oedema Diseases 0.000 description 7
- 208000006752 brain edema Diseases 0.000 description 7
- 230000003203 everyday effect Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 238000005452 bending Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000001146 hypoxic effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000009323 psychological health Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000005368 silicate glass Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an animal hypoxia-sensitive individual screening device, which comprises: a container for holding an individual animal; a cover for closing the upper port of the container; an oxygen sensor having a head extending into the container for detecting an oxygen content within the container; a carbon dioxide sensor having a head projecting into the container for detecting the oxygen content within the container; a data processing display device electrically connected with the oxygen sensor and the carbon dioxide sensor for analyzing and displaying data information provided by the oxygen sensor and the carbon dioxide sensor; wherein: an outward flange is formed at the edge of the upper port of the container, a rubber sealing strip is arranged on the outward flange, and the rubber sealing strip extends along the outward flange to form a circle; the cover body is pressed against the rubber sealing strip.
Description
Technical Field
The invention relates to the technical field of animal experiments, in particular to an animal hypoxia-sensitive individual screening device.
Background
Hypoxia includes pathological hypoxia caused by a disorder of the tissue oxygen supply system and hypoxia caused by the natural environment, such as enclosed spaces and access to high altitude areas. The hypoxia seriously affects the physical and psychological health of people, resulting in the reduction of the working efficiency. Particularly, with the needs of work and the rise of the tourism industry, more and more people go to high altitude areas, how to effectively judge the difference of the individual to hypoxia tolerance, and the establishment of an early warning mechanism for hypoxia injury becomes a research hotspot of people.
The animal hypoxia-sensitive individual screening device is used for screening hypoxia-sensitive individuals of small animals, judging the tolerance capability of the small animals to hypoxia (hypoxia in a closed space and hypoxia in a plateau), and providing a model basis for screening hypoxia-sensitive human bodies of crowds.
The individual screening device of animal hypoxia sensitivity among the prior art has an open container and the lid of closing cap on the container, because container and lid are made by glass material more, and the roughness of the last port of container is difficult to control, leads to lid and container sealed effect not good, and this makes the animal can not carry out hypoxia sensitivity screening in inclosed environment.
Disclosure of Invention
In view of the above technical problems in the prior art, embodiments of the present invention provide an animal hypoxia-sensitive individual screening device.
In order to solve the technical problem, the embodiment of the invention adopts the following technical scheme:
an animal hypoxia-sensitive individual screening device comprising:
a container for holding an individual animal;
a cover for closing the upper port of the container;
an oxygen sensor having a head extending into the container for detecting an oxygen content within the container;
a carbon dioxide sensor having a head projecting into the container for detecting the oxygen content within the container;
a data processing display device electrically connected with the oxygen sensor and the carbon dioxide sensor for analyzing and displaying data information provided by the oxygen sensor and the carbon dioxide sensor; wherein:
an outward flange is formed at the edge of the upper port of the container, a rubber sealing strip is arranged on the outward flange, and the rubber sealing strip extends along the outward flange to form a circle; the cover body is pressed against the rubber sealing strip.
Preferably, a backing plate is further arranged on the outward turning edge, and the backing plate is adhered to the outward turning edge; wherein:
the backing plate is made of a nylon plate;
the base plate is provided with an embedding groove, and the rubber sealing strip is embedded in the embedding groove.
Preferably, the animal hypoxia-sensitive individual screening device further comprises a plurality of clamping pieces, and the plurality of clamping pieces are arranged along the upper flanging at intervals; wherein:
each clamping piece is used for clamping the lower plate surface of the flanging and the upper plate surface of the cover body.
Preferably, the clamping piece comprises two clamping arms, and the two clamping arms clamp the lower plate surface of the flanging and the upper plate surface of the cover body through elastic deformation.
Preferably, the free ends of the gripping arms are formed with bends.
Preferably, two insert cylinders are formed on the inner side of the side wall of the container; wherein:
sealing rings are sleeved on the oxygen sensor and the carbon dioxide sensor;
the heads of the oxygen sensor and the carbon dioxide sensor are respectively inserted into the two inserting cylinders.
Compared with the prior art, the animal hypoxia-sensitive individual screening device disclosed by the invention has the beneficial effects that:
the lid has formed comparatively strict sealed relation with the container, and then avoids outside air to enter into the container and influence the monitoring to oxygen and carbon dioxide concentration in the container.
The summary of various implementations or examples of the technology described in this disclosure is not a comprehensive disclosure of the full scope or all features of the disclosed technology.
Drawings
In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments, by way of example and not by way of limitation, and together with the description and claims, serve to explain the inventive embodiments. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.
Fig. 1 is a schematic structural diagram of an animal hypoxia-sensitive individual screening device provided by an embodiment of the invention.
Fig. 2 is an enlarged view of a portion a of fig. 1.
Fig. 3 is an enlarged view of a portion B of fig. 1.
FIG. 4 is a graph of rat O in the control group and the cerebral edema model group2And CO2Comparing the concentration change of (c); wherein the abscissa represents time and the ordinate is O in air2And CO2Concentration percentage, n is 5.
FIG. 5 shows rats O in control group and intermittent hypoxia training group2And CO2Comparing the concentration change of (c); wherein the abscissa represents time and the ordinate is O in air2And CO2Concentration percentage, n is 5.
Reference numerals:
10-a container; 11-flanging; 12-inserting a barrel; 20-a cover body; 30-an oxygen sensor; 31-a head; 40-a carbon dioxide sensor; 41-a head; 50-a data processing display device; 60-a clamp; 61-a gripper arm; 62-bending part; 70-sealing ring; 80-a backing plate; 90-rubber sealing strip.
Detailed Description
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in the present application do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
To maintain the following description of the embodiments of the present invention clear and concise, a detailed description of known functions and known components of the invention have been omitted.
The embodiment of the invention discloses an animal hypoxia-sensitive individual screening device, which is used for drilling a peripheral soil body of a communication channel with a cross section similar to a circle.
As shown in fig. 1 to 3, the apparatus for screening an animal hypoxia-sensitive individual comprises: the container 10, the cover body 20, the clamping member 60, the rubber sealing strip 90, the oxygen sensor 30, the carbon dioxide sensor 40 and the data processing and displaying device 50.
The container 10 is made of organic glass, the container 10 is in a transparent state, an outward flange 11 is formed at the upper port of the container 10, a backing plate 80 made of nylon material is adhered to the outward flange 11, and the backing plate 80 is arranged in a circle along the outward flange 11. The upper plate surface of the backing plate 80 is provided with an embedding groove, and the embedding groove extends for a circle along the upper flanging.
The rubber sealing strip 90 is matched with the extending direction of the embedding groove, the rubber sealing strip 90 is embedded in the embedding groove, and part of the rubber sealing strip protrudes out of the backing plate 80.
A cover plate made of silicate glass is provided at the upper port of the container 10 and is overlapped on the rubber sealing strip 90.
The clamping member 60 is formed by bending a metal plate strip to form two clamping arms 61, a bent portion 62 is formed at a free end of each clamping arm 61, the clamping member 60 clamps the upper plate surface of the cover body 20 and the lower plate surface of the outward flange 11 by elastic deformation of the elastic arms, and then the cover plate is pressed against the rubber sealing strip 90 with a certain pre-tightening force, so that a tight closed space is formed in the container 10 to prevent external air from entering the container 10.
Two insert cylinders 12 are formed on the inner side wall of the container 10, the head 31 of the oxygen sensor 30 and the head 42 of the carbon dioxide sensor 40 are provided with sealing rings 70, and the heads of the oxygen sensor 30 and the carbon dioxide sensor 40 are respectively inserted into the two insert cylinders 12, so that the oxygen sensor 30 and the carbon dioxide sensor 40 are respectively used for detecting the concentration data of oxygen and carbon dioxide in the container 10.
A data processing display device 50 is electrically connected to the oxygen sensor 30 and the carbon dioxide sensor 40 for analyzing and displaying the concentrations of oxygen and carbon dioxide within the container 10.
The animal hypoxia-sensitive individual screening device provided by the invention has the advantages that:
the lid 20 forms a relatively tight seal with the container 10 to prevent outside air from entering the container 10 and affecting the monitoring of the oxygen and carbon dioxide concentrations within the container 10.
Two experiments using the above-described apparatus are described below.
Experiment 1 screening of hypoxia-sensitive individuals in small animals (rats for example):
1. the SD rat as a qualified experimental animal is selected, male, purchased from a certain company, raised in a constant-temperature (23 +/-2 ℃) and constant-humidity animal room, and can freely eat and feed water in 12/12h day and night circulation every day.
2. 10 rats were randomly divided into two groups, a control group and an LPS-treated group, each of which was 5. The cerebral edema model group was intraperitoneally injected with 4mg/kg LPS 6 hours before being placed in the device, and the control group was intraperitoneally injected with 0.9% physiological saline equal in volume to the cerebral edema model group 6 hours before being placed in the device.
3. Rats were placed in an animal hypoxia-sensitive individual screening device and timing was started and changes in the concentrations of O2 and CO2 on the display screen were recorded every 5 minutes.
4. After 30 minutes, the rats were removed, cleaned of the experimental setup, wiped clean with 75% alcohol, and a second rat screening experiment was performed until the concentrations of O2 and CO2 in the hypoxia-sensitive individual screening experimental setup were restored to normal levels.
The experimental results are shown in fig. 4, and the cerebral edema model group has a slower rate of decrease in the concentration of O2 and a slower rate of increase in the concentration of CO2 than the control group. There are 3 parameters that can be used as the index of different hypoxia-sensitive population groups, and firstly, the brain edema model group has a slower rate of decrease of the concentration of O2 compared with the control group; secondly, the rising rate of CO2 in the cerebral edema model group is slower than that in the control group; third, the time lag for the brain edema model group to reach the intersection of the O2 concentration and the CO2 concentration was compared to the control group.
Example 2 screening of Small animals for hypoxia-tolerant individuals (rat as an example)
1. The SD rat as a qualified experimental animal is selected, male, purchased from a certain company, raised in a constant-temperature (23 +/-2 ℃) and constant-humidity animal room, and can freely eat and feed water in 12/12h day and night circulation every day. A company of a low-pressure and low-oxygen animal experiment cabin simulating plateau environment. The chamber is connected with two air pumps, so that air in the chamber can be continuously pumped out of the chamber, and a low-pressure and low-oxygen environment at a certain altitude can be simulated; and is connected with an electronic display screen and an oxygen sensing probe (for displaying the altitude and the oxygen concentration in the cabin in real time).
2. 10 rats were randomly divided into two groups, a control group and an intermittent hypoxic training group (IHT group), each of which was 5. The IHT group is placed in a low oxygen chamber for 4 hours every day, and parameters are set to simulate the altitude of 3000 meters in plateau, and the ascending speed and the descending speed are both 30 m/s. After 14 days of indirect hypoxia training, a hypoxia-sensitive individual screening experiment was performed.
3. Rats were placed in the hypoxia-sensitive individual screening test device while a timer was pressed and changes in the concentrations of O2 and CO2 on the display screen were recorded every 5 minutes.
After 4.30 minutes, the rats were removed, the experimental setup cleaned, wiped clean with 75% alcohol, and a second rat screening experiment was performed until the concentrations of O2 and CO2 in the hypoxia-sensitive individual screening experimental setup were restored to normal levels.
The results of the experiment are shown in FIG. 5, in which the IHT group showed a faster decrease in O2 concentration and a faster increase in CO2 concentration than the control group. There are 3 parameters that can be used as indicators for different hypoxia-sensitive groups, wherein, firstly, the IHT group has a faster decrease rate of O2 concentration than the control group; secondly, the rising rate of the concentration of CO2 in the IHT group is faster than that in the contrast group; third, the IHT group and the comparative group reached the time point at which the O2 concentration crossed the concentration of CO 2.
The experiment proves that the device can be used as an experimental device for screening the hypoxia-sensitive individuals of the small animals, and the method for screening the hypoxia-sensitive individuals of the small animals by using the experimental device is simple and easy to operate.
Moreover, although exemplary embodiments have been described herein, the scope of the present invention includes any and all embodiments based on the present invention with equivalent elements, modifications, omissions, combinations (e.g., of various embodiments across), adaptations or alterations. The elements of the claims are to be interpreted broadly based on the language employed in the claims and not limited to examples described in the present specification or during the prosecution of the application, which examples are to be construed as non-exclusive. It is intended, therefore, that the specification and examples be considered as exemplary only, with a true scope and spirit being indicated by the following claims and their full scope of equivalents.
The above description is intended to be illustrative and not restrictive. For example, the above-described examples (or one or more versions thereof) may be used in combination with each other. For example, other embodiments may be used by those of ordinary skill in the art upon reading the above description. In addition, in the above-described embodiments, various features may be grouped together to streamline the disclosure. This should not be interpreted as an intention that a disclosed feature not claimed is essential to any claim. Rather, inventive subject matter may lie in less than all features of a particular disclosed embodiment. Thus, the following claims are hereby incorporated into the detailed description as examples or embodiments, with each claim standing on its own as a separate embodiment, and it is contemplated that these embodiments may be combined with each other in various combinations or permutations. The scope of the invention should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled.
The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the scope of the present invention is defined by the claims. Various modifications and equivalents may be made by those skilled in the art within the spirit and scope of the present invention, and such modifications and equivalents should also be considered as falling within the scope of the present invention.
Claims (6)
1. An animal hypoxia-sensitive individual screening device, comprising:
a container for holding an individual animal;
a cover for closing the upper port of the container;
an oxygen sensor having a head extending into the container for detecting an oxygen content within the container;
a carbon dioxide sensor having a head projecting into the container for detecting the oxygen content within the container;
a data processing display device electrically connected with the oxygen sensor and the carbon dioxide sensor for analyzing and displaying data information provided by the oxygen sensor and the carbon dioxide sensor; wherein:
an outward flange is formed at the edge of the upper port of the container, a rubber sealing strip is arranged on the outward flange, and the rubber sealing strip extends along the outward flange to form a circle; the cover body is pressed against the rubber sealing strip.
2. The animal hypoxia-sensitive individual screening device according to claim 1, wherein a backing plate is further disposed on the outward turning edge, and the backing plate is adhered to the outward turning edge; wherein:
the backing plate is made of a nylon plate;
the base plate is provided with an embedding groove, and the rubber sealing strip is embedded in the embedding groove.
3. The animal hypoxia-sensitive individual screening device according to claim 1, further comprising a plurality of clamping pieces, wherein the plurality of clamping pieces are arranged at intervals along the upper flange; wherein:
each clamping piece is used for clamping the lower plate surface of the flanging and the upper plate surface of the cover body.
4. The animal hypoxia-sensitive individual screening device according to claim 3, wherein the clamping member includes two clamping arms that clamp the lower plate surface of the flange and the upper plate surface of the cover by elastic deformation.
5. The animal hypoxia-sensitive individual screening device according to claim 4, wherein the free end of the holding arm is formed with a bent portion.
6. The animal hypoxia-sensitive individual screening device according to claim 1, wherein two insertion cylinders are formed inside the side wall of the container; wherein:
sealing rings are sleeved on the oxygen sensor and the carbon dioxide sensor;
the heads of the oxygen sensor and the carbon dioxide sensor are respectively inserted into the two inserting cylinders.
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CN202110502333.4A CN113229156A (en) | 2021-05-08 | 2021-05-08 | Animal hypoxia-sensitive individual screening device |
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CN202110502333.4A CN113229156A (en) | 2021-05-08 | 2021-05-08 | Animal hypoxia-sensitive individual screening device |
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CN207877739U (en) * | 2017-12-23 | 2018-09-18 | 湘南学院 | A kind of preventive medicine bacteria detecting apparatus |
CN210063754U (en) * | 2019-05-08 | 2020-02-14 | 东莞市森本模具塑胶有限公司 | Environment-friendly recyclable packaging container filled with air |
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JP2006288275A (en) * | 2005-04-11 | 2006-10-26 | Nakata Coating Co Ltd | Oxygen-keeping container for animal |
CN101238041A (en) * | 2005-08-05 | 2008-08-06 | 麒麟麦酒株式会社 | Sealed container |
US20110023789A1 (en) * | 2007-12-10 | 2011-02-03 | The Cooper Union For The Advancement Of Science And Art | Gas delivery system for an animal storage container |
CN101889908A (en) * | 2010-07-06 | 2010-11-24 | 首都医科大学附属北京朝阳医院 | Gas atomization toxicant exposure box |
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CN103021249A (en) * | 2012-12-31 | 2013-04-03 | 东莞市人民医院 | Heating carbon dioxide simulating abdominal cavity experimental box |
CN107331806A (en) * | 2016-04-29 | 2017-11-07 | 宁德时代新能源科技股份有限公司 | Box body sealing structure |
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Application publication date: 20210810 |