CN113214996B - Scenedesmus as well as culture method and application thereof - Google Patents
Scenedesmus as well as culture method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a Scenedesmus and a culture method and application thereof, wherein the Scenedesmus is Scenedesmus sp.TXH202001, which is deposited in China center for type culture Collection on 12.01.2021, and the deposit numbers are as follows: CCTCC NO: M2021047. The invention discovers that when the scenedesmus is cultured into imidacloprid with different concentration gradients, the biomass, chlorophyll fluorescence parameters, the total chlorophyll and carotenoid content, the superoxide dismutase content and the malondialdehyde content of the scenedesmus are not obviously changed, namely the scenedesmus can tolerate the imidacloprid with high concentration. The change of the concentration of the imidacloprid is measured to find that the scenedesmus has obvious effect of removing the imidacloprid, the removal rate of the scenedesmus is gradually increased along with the increase of the culture time, and the highest removal rate can reach 99.63 percent when the scenedesmus is cultured to the 18 th day. Therefore, the scenedesmus can be applied to imidacloprid polluted water to remove imidacloprid pollution, protect water quality and various water organisms and maintain ecological balance.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to scenedesmus and a culture method and application thereof.
Background
Scenedesmus, also known as Scenedesmus, a genus of Scenedesmoideae of the order Chlorococcales of the phylum Chlorophytum, usually consists of a fixed population of 4-8 cells, sometimes 16-32 cells, rarely single cells. Scenedesmus is a common planktonic alga in fresh water and is extremely favored to be propagated in still water with rich nutrition. The algae grows in various still water bodies such as lakes, reservoirs, ponds, water pits, swamps and the like and is common important planktonic algae. Many of the species are reported to have strong tolerance to organic pollutants, play a certain role in water self-purification and sewage purification, are dominant species in the biological phase of the organic sewage oxidation pond, and can be used as indicator organisms in water quality evaluation. The scenedesmus has a certain effect in self-purification of water and sewage purification, and can be attached to organic matter fragments and other aquatic plant bodies in water together with bacteria to form a colloidal layer for adsorbing organic matters. When the scenedesmus is subjected to photosynthesis, on one hand, oxygen is generated to meet the requirement of bacteria on decomposing organic matters, and on the other hand, the organic matters can be directly used as a carbon source and a nitrogen source to rapidly degrade the organic matters in water, so that the water body is purified.
Imidacloprid is a nitro methylene systemic insecticide, belonging to chloronicotinyl insecticides, also known as neonicotinyl insecticides. The pesticide is a broad-spectrum efficient pesticide, has multiple effects of contact killing, stomach toxicity, systemic absorption and the like, is an action body of a nicotine acetylcholine receptor, interferes the motor nervous system of pests to cause the transmission failure of chemical signals, has no cross resistance problem, is mainly used for preventing and treating pests with piercing-sucking mouthparts, and is a pesticide with wide application. However, researches find that imidacloprid has potential harm to water environment, has obvious influence on plankton communities, large invertebrates, partial phytoplankton and water quality in water, and has persistence and is not easy to degrade in the water environment. Therefore, the method has important significance for removing the residual imidacloprid in the water environment.
Disclosure of Invention
The invention aims to provide a Scenedesmus sp.TXH202001 which is separated and purified from activated sludge, mixed with imidacloprid with a certain concentration gradient and found that the imidacloprid has no inhibition effect on the growth of the Scenedesmus, and further the Scenedesmus has a remarkable removal effect on the imidacloprid with the removal rate of more than 99 percent.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides Scenedesmus sp.TXH202001, which is preserved in China center for type culture Collection in 2021, 01, 12 and has the address as follows: the preservation number of the Scenedesmus sp.TXH202001 is as follows: CCTCC NO: M2021047.
The invention also provides a culture method of the scenedesmus, which comprises the steps of inoculating the scenedesmus into a BG-11 culture medium, and culturing at the temperature of 25 +/-1 ℃.
Further, the light intensity during the culture was 40. Mu. Mol/m 2 /s。
Further, the light-dark ratio during the culture process is 12h: and (4) 12h.
Further, the oxygen aeration during the culture was 0.6L/min.
The invention also provides application of the scenedesmus in resisting imidacloprid.
The invention also provides application of the scenedesmus to imidacloprid removal.
Further, the concentration of the imidacloprid is 5-150 mg/L.
The invention also provides a method for treating imidacloprid-polluted water, which comprises the following steps: adding the scenedesmus into imidacloprid sewage.
Further, the scenedesmus is scenedesmus cultured to the logarithmic growth phase.
Compared with the prior art, the invention has the beneficial effects that: the invention obtains a Scenedesmus strain by separating and purifying activated sludge, which is named Scenedesmus sp.TXH202001 and is preserved in China center for type culture collection in 2021, 01, 12 days, wherein the preservation number is as follows: CCTCC NO: M2021047. After the scenedesmus is subjected to amplification culture to a logarithmic phase, imidacloprid with a certain concentration gradient is added, the change of the physicochemical characteristics of the imidacloprid is observed, and the biomass, chlorophyll fluorescence parameters, the total chlorophyll and carotenoid content, the superoxide dismutase content and the malonaldehyde content of the scenedesmus are not obviously changed, namely the scenedesmus can tolerate the imidacloprid with high concentration. Furthermore, the concentration of imidacloprid is measured to find that the scenedesmus has an obvious effect of removing imidacloprid, the removal rate of the scenedesmus is gradually increased along with the increase of the culture time, and the highest removal rate can reach 99.63 percent when the scenedesmus is cultured to 18 days. Therefore, the scenedesmus can be applied to imidacloprid polluted water to remove imidacloprid pollution, protect water quality and various water organisms and maintain ecological balance.
Drawings
FIG. 1 is a linear relationship graph of optical density values and dry cell weights of algal solutions in example 2 of the present invention;
FIG. 2 is the dry weight change of scenedesmus in imidacloprid with different concentration gradients in example 2 of the present invention;
FIG. 3 shows the change of chlorophyll fluorescence parameters of Scenedesmus obliquus in imidacloprid with different concentrations in example 2 of the present invention;
FIG. 4 shows the total chlorophyll content of Scenedesmus obliquus in imidacloprid of different concentrations in example 2 of the invention;
FIG. 5 shows the carotenoid content of scenedesmus in different concentrations of imidacloprid in example 2 of this invention;
FIG. 6 shows the SOD content variation of Scenedesmus in imidacloprid with different concentrations in example 2 of the present invention;
FIG. 7 shows the MDA content variation of scenedesmus in different concentrations of imidacloprid in example 2 of the present invention;
FIG. 8 shows the effect of scenedesmus on the removal of imidacloprid with different concentration gradients in example 2 of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 Scenedesmus acquirement, culture and acclimatization
1. Scenedesmus acquirement
(1) Preparing a BG-11 culture medium:
①NaNO 3 solution: 75.0000gNaNO 3 Dissolving in water to a constant volume of 500mL;
②CaCl 2 solution: 2.7180g of the extract is dissolved in water to be 100mL;
③K 2 HPO 4 solution: 4.0000g of K 2 HPO 4 ·3H 2 Dissolving O in water to reach a constant volume of 100mL;
④MgSO 4 solution: 7.5000g MgSO 4 ·7H 2 Dissolving O in water to reach the constant volume of 100mL
(5) Ferric ammonium citrate: 0.6000g of ammonium ferric citrate is dissolved in water to be 100mL
(6) Citric acid solution: 0.6000g of citric acid is dissolved in water to reach a constant volume of 100mL
⑦NaCO 3 Solution: 2.0000g NaCO 3 Dissolving in water to reach a constant volume of 100mL
⑧EDTANa 2 Solution: 0.1000g EDTA-Na 2 Dissolving in water to reach a constant volume of 100mL
(9) Solution A5: 0.2860g H 3 BO 3 、0.1860g MnCl 2 ·4H 2 O、0.0220g ZnSO 4 ·7H 2 O、0.0390g Na 2 MoO 4 ·2H 2 O、0.0080g CuSO 4 ·5H 2 O、0.0050g Co(NO 3 ) 2 ·6H 2 Dissolving O in water one by one, and fixing the volume to 100mL;
taking 10mLNaNO 3 The solution, components (2) to (9), were each 1ml, and 400 μ L of NaOH solution was adjusted to pH =7 and the volume was adjusted to 1L to obtain BG-11 medium.
(2) Sampling in Tangson lake of Wuhan City, inoculating in sterilized BG-11 solid culture medium by sucking water and activated sludge water sample with sterile suction tube, dripping 2-3 drops in each plate, sealing the plates with sealing film, and placing in an inverted state at 25 + -1 deg.C and illumination intensity of 40 μmol/m 2 And/s, culturing in an illumination incubator with the light-dark ratio of 12h to 12h, and obtaining the mixed microalgae community after 7-10 days. Observing microalgae community, repeating plate streaking inoculation culture for 8-10 times until obtaining purified cultured single algae colony, and separating and purifying to obtain 4 kinds of microalgae.
One of the microalgae is selected, the morphology of the microalgae is observed under a microscope, the microalgae is preliminarily classified and identified by combining Chinese freshwater algae-system, classification and ecology, and the colony morphology, biochemistry and 18S rDNA and ITS combined sequencing analysis and identification are carried out.
The method specifically comprises the following steps: after the genomic DNA of a sample to be detected is crudely extracted, a corresponding primer is selected to amplify a specific fragment, a TSINGKE DNA gel recovery kit (Code No. GE0101) is used for cutting gel and recovering a target fragment, the sequencing is carried out by the corresponding primer,
wherein the primers for amplifying the 18s rDNA are as follows:
HET-F：5′-ACCTGGTTGATCCTGCCAGTAGTCATAC-3′；
HET-R：5′-GGTTCACCTACGGAAACCTTGTTACGACTTCA-3′。
primers used to amplify the ITS region were:
ITS1：5′-TCCGTAGGTGAACCTGCGG-3′；
ITS4：5′-TCCTCCGCTTATTGATATGC-3′。
the amplified sequences were aligned with the NCBI database. The comparison result showed that the strain was Scenedesmus sp (Scenedesmus sp.), which was named Scenedesmus sp.TXH202001. The Scenedesmus has been deposited in China Center for Type Culture Collection (CCTCC) at 12.01.2021, with the preservation number of M2021047, which is named Scenedesmus sp.TXH202001 by classification, and the preservation unit address is: wuhan university in Wuhan City of Hubei province of China.
2. Cultivation of Scenedesmus
The separated and purified Scenedesmus sp.TXHH202001 strain is transferred to a 150mL sterile conical flask filled with sterilized BG-11 liquid culture medium for seed preservation and culture. After the algae grows to the logarithmic growth phase, transferring part of algae liquid into a 2L sterile conical flask for amplification culture, setting the culture temperature of an illumination incubator to be 25 +/-1 ℃, and setting the illumination intensity to be 40 mu mol/m 2 The light-dark ratio is 12h to 12h, and the ventilation volume is 0.6L/min.
3. Domestication of Scenedesmus sp.TXH202001
Culturing Scenedesmus sp.TXHH202001 to logarithmic phase, collecting appropriate amount of algae solution, and diluting with sterilized BG-11 culture medium to OD 680 About 1.0, then 10mL of the diluted algae solution is taken to be put into a 250mL sterile conical flask, and then imidacloprid (98.7%, shanghai pesticide research institute Co., ltd., china) stock solution (100 mg/L) is independently added into the algae solution to ensure that the conical shape is conicalThe total amount of the final liquid in the bottle is 200mL, the concentration gradient of imidacloprid in the algae liquid is 0, 5, 20, 50, 100 and 150mg/L, and 3 parallels are arranged in each group. Plugging the conical bottle with a cotton plug, sealing the conical bottle with kraft paper, placing the conical bottle in a light incubator at a temperature of 25 +/-1 ℃ and a light-dark ratio of 16h: the illumination intensity is 100 mu mol/m for 8h 2 /s 1 The culture was carried out for 18 days with regular shaking 3 times per day. Then, the physicochemical characteristics of the scenedesmus and the removal effect of the scenedesmus on the imidacloprid are researched and analyzed.
Example 2 Gracilaria physical and chemical Properties and Imidacloprid removal Effect
1. Scenedesmus sp.TXH202001 Dry weight determination
(1) Taking a proper amount of Scenedesmus sp.TXH202001 algae liquid cultured to the stationary phase, and diluting the Scenedesmus sp.TXH202001 algae liquid into algae liquid with different OD 680 Filtering with pre-weighed filter paper, drying at 105 deg.C, cooling to room temperature, weighing again, and calculating different OD 680 Value corresponding to the weight of the stem cells, wherein OD 680 The values were measured by UV spectrophotometer to establish the Dry Cell Weight (DCW) and Optical Density (OD) 680 ) The linear relationship between the values is shown in fig. 1. According to FIG. 1, as the weight of the algal stem cells increases, the optical density value increases correspondingly, and the optical density OD of the algal solution increases correspondingly 680 The value is in a positive linear correlation with the weight of the algae stem cells (R) 2 >0.99 Indicating that the corresponding algal biomass can be expressed by optical density values, the linear relationship is: DCW (g/L) =0.6326 × OD 680 -0.0377(R 2 ＝0.9954)。
(2) Taking Scenedesmus cultured in different concentration gradients in example 1, sampling every 3d, taking 2mL of algae solution, shaking, and measuring OD by ultraviolet spectrophotometer 680 And calculating the influence of imidacloprid with different concentrations on the dry weight of Scenedesmus sp.TXH202001 according to the linear relation. The measurement results are shown in FIG. 2.
As shown in the figure, the biomass of Scenedesmus sp.TXH202001 was increasing with time, and at each stage, the biomass of Scenedesmus sp.TXH202001 was maximal at an imidacloprid concentration of 50mg/L, and at the end of the experiment, the biomass of Scenedesmus sp.TXH202001 was higher at each concentration of imidacloprid than at the control except 20mg/L, and at 20mg/L, there was no significant decrease in Scenedesmus biomass (p > 0.05) compared to the control. Namely, imidacloprid with the concentration of less than 150mg/L has no inhibition effect on the microalgae Scenedesmus sp.TXH202001.
2. Determination of chlorophyll fluorescence parameter of Scenedesmus sp.TXH202001
Taking the scenedesmus cultured in different concentration gradients imidacloprid in example 1 as a sample to be detected, and measuring chlorophyll fluorescence parameters every 3d, specifically: the sample to be measured is put into a dark environment to be subjected to dark adaptation for 20min, and then is measured by a portable chlorophyll fluorescence instrument, and the measurement result is shown in figure 3.
Wherein the chlorophyll fluorescence parameter Fv/Fm represents the maximum photochemical yield of the PSII, and refers to the ratio of variable fluorescence to maximum fluorescence, which is the potential and maximum photosynthetic activity of plants (including algae) and reflects the maximum light energy conversion efficiency of the PSII reaction center. The Fv/Fm values of eukaryotic algae remain at a stable level when unstressed, and decrease when stressed.
According to FIG. 3, the Fv/Fm values of imidacloprid at different concentrations in the whole period are slightly increased or are consistent, are all maintained between 0.6 and 0.75, and are not significantly reduced compared with the control group, which indicates that the strain of microalgae Scenedesmus sp.TXH202001 is not stressed by imidacloprid, i.e. imidacloprid has little influence on the photosynthesis of the strain of Scenedesmus.
3. Scenedesmus sp.TXH202001 Total chlorophyll and Carotenoid content determination
Scenedesmus cultured in different concentration gradients in example 1 was taken as a sample to be tested, samples were taken every 3 days, 5mL of algal solution was taken each time, centrifuged at 8000rpm for 5 minutes, the supernatant was discarded, the precipitate was resuspended in 5mL of 90% methanol, subjected to water bath at 60 ℃ for 10 minutes in the dark, and centrifuged again for 5 minutes. The absorbance of the supernatant at 665, 652 and 470nm was then determined with a uv-vis spectrophotometer and the total chlorophyll (total chlorophyl) and carotenoid (carotenoid) contents were calculated by the following equation:
C a (mg/L)＝16.82A 665 -9.28A 652
C b (mg/L)＝36.92A 652 -16.54A 665
C car (mg/L)＝(1000A 470 -1.91C a -95.15C b )/225
C general (1) (mg/L)＝C a +C b
In the formula, C a Represents chlorophyll a content (mg/L); c b Represents the chlorophyll b content (mg/L); c car Represents the carotenoid content (mg/L), C General assembly Represents the total chlorophyll content (mg/L). The measurement results are shown in fig. 4 and 5.
Chlorophyll is a main index of electron transfer activity in photosynthesis, and is closely related to various aspects of photosynthesis, including light capture, energy transfer and light energy conversion. Chlorophyll is considered a sensitive biomarker when plant cell systems are exposed to toxic substances, and inhibition of growth of algae is associated with altered chlorophyll biosynthesis upon exposure to toxic compounds. According to fig. 4, the total chlorophyll content of the microalgae Scenedesmus sp.txh202001 gradually increased with increasing cultivation time. The total chlorophyll content is lower when the imidacloprid concentration is 20-100mg/L at the 0 th day, which probably is because certain error exists in the lower total chlorophyll content of the microalgae at the beginning of the experiment. The total chlorophyll content decreased with high imidacloprid treatment from day 12 but did not change significantly (P > 0.05) until the end of day 18.
Carotenoids play an important role in light trapping, have antioxidant properties, protect cells by quenching singlet oxygen and free radicals produced within the cell, prevent lipid peroxidation, and promote stability and functionality of photosynthetic organs, thereby providing a defense system for the photosynthetic process. According to FIG. 5, the carotenoid content was gradually increased with the increase of the culture time, similar to the change of the total chlorophyll content. The carotenoid content did not decrease significantly at day 18 with high imidacloprid treatment (P > 0.05). The results are combined, that is, imidacloprid with concentration lower than 150mg/L has no inhibiting effect on the microalgae.
4. The change of the activity of superoxide dismutase (SOD) in Scenedesmus sp.TXH202001 is considered as an important index for judging cell damage, and the increase of the SOD content indicates that the SOD of microalgae exposed in organic matters participates in the process of reducing superoxide radicals into hydrogen peroxide, and excessive ROS are accumulated in cells to cause the cell damage. Therefore, the SOD content can reflect whether the imidacloprid has damage inhibition effect on the growth of the scenedesmus. The determination method comprises the following specific steps: the activity of the superoxide dismutase is generally expressed in activity units, 50% of inhibiting photochemical reduction of Nitrobluetetrazolium (NBT) is taken as an enzyme activity unit (U), and the determination method is shown in a kit produced by the above Haiyuan leaf biotechnology limited. The calculation formula is as follows: SOD Activity (U/mL) = (A) Illumination of light -A Measurement of )/(50％×A Illumination of light ×V)
The results are shown in FIG. 6, where the SOD level was high at the beginning of the experiment, probably because the Scenedesmus had not been adapted after the addition of imidacloprid as a contaminant. In general, imidacloprid had no significant effect on the SOD content of scenesmus sp (P > 0.05), but the SOD content in the environment with imidacloprid added was higher than the control. Indicating that the microalgae exposed to high concentrations of imidacloprid were not significantly stressed.
5. Determination of content of Scenedesmus sp.TXH202001 Malondialdehyde (MDA)
The production and elimination of Reactive Oxygen Species (ROS) in plants, including algae, is in dynamic equilibrium under normal growth conditions. However, exposure to toxic contaminants can result in excessive ROS production, including superoxide radical (O) 2 -, hydroxyl radical (. OH) and hydrogen peroxide (H) 2 O 2 ). These ROS, which have strong oxidative properties, can cause fatal damage to cell organs by peroxidation of polyunsaturated fatty acids (PUFAs), which reduces membrane fluidity, increases leakage and causes secondary damage to membrane proteins. Malondialdehyde (MDA) is a representative product of aldehydes and PUFAs, and is often used as an indicator of oxidative damage to cells. Therefore, the change of the content of MDA can reflect the damage condition of imidacloprid to the scenedesmusThe method is as follows. The specific determination method comprises the following steps: 5mL of algae solution to be detected is taken, 5mL of 5% trichloroacetic acid (TCA) is added, homogenate obtained after grinding is centrifuged for 10min at 3000r/min, and supernatant is sample extracting solution. 2mL of the supernatant was taken, and 2mL of 0.67% thiobarbituric acid (TBA) was added thereto, mixed, boiled in a water bath at 100 ℃ for 30min, cooled, and centrifuged again. Respectively measuring the absorbance values of the supernatant at 450nm,532nm and 600nm, wherein the calculation formula of the malonaldehyde content is as follows: c MDA (μmoL/L)＝6.45(A 532 -A 600 )-0.56A 450 。
The results are shown in FIG. 7, where the MDA content gradually increased throughout the experiment, similar to the SOD content variation. At day 18, the MDA content of microalgae treated by imidacloprid with other concentrations was higher than that of the control group, wherein the concentration was the lowest at 50mg/L, namely the microalgae damage degree was the smallest. Compared with a control group, the change of the concentration of the imidacloprid in the experimental process has no obvious influence on the content of MDA (P is more than 0.05), namely, the strain of microalgae is hardly stressed by imidacloprid medicaments.
6. Imidacloprid removal effect of Scenedesmus sp.TXH202001
In order to verify whether the scenedesmus has the effect of removing imidacloprid, samples are taken every 3d to determine the change of the imidacloprid content in the algae liquid, and the method specifically comprises the following steps: 2mL of the algal solution was filtered through a 0.22 μm organic filter and subjected to HPLC-MS/MS (QSight) TM LX50/210, perkinelmer), phenomenex Kinetic C18A (4.6X 100mm,2.6 μm), mobile phase A0.1% formic acid (V/V), mobile phase B acetonitrile, mobile phase A, B ratio 40:60, setting the flow rate to be 0.6mL/min, setting the column temperature to be 35 ℃, setting the sample injection amount to be 1 mu L, setting the sample injection time to be 3min, and setting the imidacloprid ion peak retention time to be 1.69min. Then, the change in imidacloprid content was calculated as the removal rate. The measurement results are shown in FIG. 8.
According to FIG. 8, the removal rate of imidacloprid at each concentration gradually increased with the increase of the culture time, i.e., the removal capacity of Scenedesmus sp.TXH202001 strain to imidacloprid gradually increased with the increase of the culture time, and the Scenedesmus sp.TXH202001 strain had the best removal effect to 50mg/L imidacloprid at each culture stage. The removal rate is as high as 99.63% by the day 18 of culture. Namely, the Scenedesmus sp.TXH202001 has good effect of removing imidacloprid, and has wide application prospect and high application value.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (10)
1. The Scenedesmus is Scenedesmus sp.TXH202001 with the preservation number as follows: CCTCC NO: M2021047.
2. The method for culturing Scenedesmus as claimed in claim 1, wherein the Scenedesmus is inoculated into BG-11 medium and cultured at a temperature of 25 ± 1 ℃.
3. The culture method according to claim 2, wherein the light intensity during the culture is 40. Mu. Mol/m 2 /s。
4. The culture method according to claim 2, wherein the light-to-dark ratio during the culture is 12h: and (4) 12h.
5. The culture method according to claim 2, wherein the oxygen flow rate during the culture is 0.6L/min.
6. Use of a scenedesmus as claimed in claim 1 for the tolerance of imidacloprid.
7. Use of a scenedesmus as claimed in claim 1 for the removal of imidacloprid.
8. The use according to claim 6 or 7, characterized in that the imidacloprid concentration is between 5 and 150mg/L.
9. A method of treating imidacloprid contaminated water, which comprises: adding the scenedesmus of claim 1 to imidacloprid wastewater.
10. The method of claim 9, wherein the scenedesmus is scenedesmus cultivated to the log phase of growth.
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| CN101565674A (en) * | 2009-05-13 | 2009-10-28 | 清华大学 | Low-nutrition scenedesmus and application thereof in sewage deep treatment |
| US20150299646A1 (en) * | 2011-12-22 | 2015-10-22 | Stc.Unm | Cultured extremophilic algae species native to new mexico |
| CN102978114B (en) * | 2012-09-14 | 2014-08-13 | 新奥科技发展有限公司 | Scenedesmus sp. and applications thereof |
| CN103087920B (en) * | 2013-01-24 | 2014-10-08 | 清华大学 | Mixotrophic scenedesmus and application thereof in sewage resource treatment |
| CN104388315A (en) * | 2014-11-07 | 2015-03-04 | 山东大学 | Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof |
| CN104593265B (en) * | 2015-01-29 | 2019-01-29 | 山东大学 | One plant height oil-producing scenedesmus and its screening, cultural method and application |
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