CN113208950A - Tea tree oil-containing ear canal cleanser for pets - Google Patents
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Abstract
The invention relates to a tea tree oil-containing auditory canal cleanser for pets, which comprises the following components in parts by weight: 5-20 parts of tea tree oil/casein modified microspheres, 4-10 parts of glycerol, 2-6 parts of cellulose derivatives and 50-100 parts of deionized water. The invention discloses an auditory canal cleanser containing tea tree oil for pets, which comprises tea tree oil/casein modified microspheres, glycerol and cellulose derivatives as main components, wherein the tea tree oil/casein modified microspheres have the effects of sterilizing, relieving pain and diminishing inflammation, the glycerol has the effects of lubricating and moistening, and the cellulose derivatives have the effects of cleaning and maintaining liquid balance. The tea tree oil-containing auditory canal cleanser for pets, which is prepared by the invention, is simple in ingredients, environment-friendly, mild in performance, less in irritation and strong in sterilizing and cleaning effects, and can play a role in easing pain and diminishing inflammation.
Description
Technical Field
The invention relates to the field of pet products, and in particular relates to a tea tree oil-containing ear canal cleanser for pets.
Background
With the improvement of living standard and living quality of people, pets gradually become an indispensable part in our lives. However, pets are fun and a link which needs to be paid the most attention to the health and care of pets. Earwax, earwax or necrotic corneocytes are easy to accumulate in the ears of the pets, and if the dirt in the auditory canals is not removed in time, bacterial infection in the auditory canals of the pets can be caused, various diseases of the auditory canals of the pets, such as otitis externa, otitis media and the like, are caused, so that the resistance of the pets is reduced, and other diseases are caused. However, in the existing ear canal cleaning agent for pets, the ear canal cleaning bacteriostatic agent has poor bacteriostatic effect and short efficacy duration.
Disclosure of Invention
Aiming at the problems in the background art, the invention provides tea tree oil-containing ear canal cleanser for pets, which comprises the following components in parts by weight:
5-20 parts of tea tree oil/casein modified microspheres, 4-10 parts of glycerol, 2-6 parts of cellulose derivatives and 50-100 parts of deionized water.
Preferably, the ear canal cleanser comprises the following components in parts by weight:
8-15 parts of tea tree oil/casein modified microspheres, 5-8 parts of glycerol, 3-5 parts of cellulose derivatives and 65-80 parts of deionized water.
Preferably, the ear canal cleanser comprises the following components in parts by weight:
5-20 parts of tea tree oil/casein modified microspheres, 4-10 parts of glycerol, 2-6 parts of cellulose derivatives and 50-100 parts of deionized water.
Preferably, the particle size of the tea tree oil/casein modified microspheres is 20-100 microns.
Preferably, the cellulose derivative comprises at least one of methyl cellulose, carboxymethyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose.
Preferably, the preparation method of the casein modified microspheres comprises the following steps:
s1, weighing casein and deionized water, sequentially adding the casein and the deionized water into a reaction container, dropwise adding sodium hydroxide until the pH value of reaction liquid is 9.5-10.5, and fully stirring and completely dissolving to obtain a casein solution;
wherein the concentration of casein in the casein solution is 50-100 g/L;
s2, adding sophocarpine into an ethanol solution with the mass fraction of 30-70%, and fully stirring and completely dissolving to obtain a sophocarpine solution;
wherein the mass ratio of the sophocarpine to the ethanol solution is 1: 12-16;
s3, placing the reaction container filled with the casein solution into a water bath at 40-60 ℃, stirring at the speed of 300-500 rpm, continuously dropwise adding the sophocarpine solution, continuously stirring for 2-5 hours after all the sophocarpine solution is dropwise added, and cooling to room temperature to obtain a mixed reaction solution;
wherein the volume ratio of the casein solution to the sophocarpine solution is 1: 1.2-1.6;
s4, weighing tea tree oil and dichloromethane, mixing the tea tree oil and the dichloromethane into a reactor, fully stirring and dissolving, and then adding acetic acid to adjust the pH value of the liquid to 4.0-5.0 to obtain a tea tree oil solution;
wherein the mass ratio of the tea tree oil to the dichloromethane is 1: 5-8;
s5, dropwise adding the mixed reaction liquid into the tea tree oil solution at a stirring speed of 300-500 rpm, continuously adding acetic acid in the dropwise adding process to keep the pH value of the liquid in the reactor at 4.0-5.0, continuously stirring for 1-4 h after all the mixed reaction liquid is added, standing for 5-10 h, centrifugally collecting solid precipitates, washing the precipitates with distilled water for at least three times, and performing freeze drying treatment to obtain tea tree oil/casein modified microspheres;
wherein the volume ratio of the tea tree oil solution to the mixed reaction liquid is 1: 2.2-2.8.
The invention has the beneficial effects that:
1. the invention discloses an auditory canal cleanser containing tea tree oil for pets, which comprises tea tree oil/casein modified microspheres, glycerol and cellulose derivatives as main components, wherein the tea tree oil/casein modified microspheres have the effects of sterilizing, relieving pain and diminishing inflammation, the glycerol has the effects of lubricating and moistening, and the cellulose derivatives have the effects of cleaning and maintaining liquid balance. The tea tree oil-containing auditory canal cleanser for pets, which is prepared by the invention, is simple in ingredients, environment-friendly, mild in performance, less in irritation and strong in sterilizing and cleaning effects, and can play a role in easing pain and diminishing inflammation.
2. The invention uses casein and sophocarpine to form microspheres, and wraps the tea tree oil into the microspheres in an oil-in-water mode, so that the tea tree oil is not volatilized completely in a short time, and the effects of pain relieving and inflammation diminishing of sophocarpine can be achieved. As the casein is a completely biodegradable material and has no harm to organisms, the small molecular polypeptide obtained by degrading the casein in the microspheres can be gradually released by degrading the encapsulated tea tree oil through the casein, so that the tea tree oil can play an antibacterial and bactericidal role in the auditory canals of pets for a longer time.
3. The invention uses sophocarpine to modify casein, so as to prepare the shell of the oil-in-water microsphere. The alkaline tertiary amine bond in sophocarpine can increase the polarity of hydrophobic domain of hydrophobic amino acid residue, thereby increasing hydrophilicity, and thus can be more easily stably existed in water solution. Then the oleophilic tea tree oil is wrapped in the microsphere shell to form the tea tree oil/casein modified microsphere with the functions of slow release, antibiosis, anti-inflammation and analgesia.
Detailed Description
For the purpose of more clearly illustrating the present invention and more clearly understanding the technical features, objects and advantages of the present invention, the technical solutions of the present invention will now be described in detail below, but are not to be construed as limiting the implementable scope of the present invention.
Tea tree oil is an important pharmaceutical chemical raw material, and can efficiently kill bacteria and viruses on the surface of skin. The application in antibiosis is quite wide, and more than 100 first-aid purposes exist. The tea tree oil has unique sterilization and cleaning effects, has broad-spectrum antibacterial and bacteriostatic effects, has antibacterial and bacteriostatic effects on escherichia coli and staphylococcus aureus, and also has strong sterilization effects on viruses and fungi. However, tea tree oil is a volatile oil and is extremely insoluble in water, which greatly limits the use and storage of the cleanser.
Sophocarpine is one of the main alkaloids contained in Sophora alopecuroides L of Leguminosae, has antibacterial and insecticidal effects, and has effects of inhibiting central nerve, relieving pain and diminishing inflammation, and its molecule contains alkaline tertiary amine bond.
Casein, as a natural protein, is a renewable resource that is completely biodegradable. Because of unique film-forming property and the structure contains various active groups and is easy to modify, casein film-forming substances have been widely used, however, although casein contains a large number of hydrophilic groups and molecular secondary bonds, casein also contains a large number of hydrophobic amino acid residues (proline residues), so that the water solubility of the casein is poor.
The invention is further described below with reference to the following examples.
Example 1
The tea tree oil-containing auditory canal cleanser for pets comprises the following components in parts by weight:
16 parts of tea tree oil/casein modified microspheres, 8 parts of glycerol, 4 parts of cellulose derivatives and 80 parts of deionized water.
The particle size of the tea tree oil/casein modified microspheres is 20-100 mu m.
The cellulose derivative is methyl cellulose.
The preparation method of the casein modified microspheres comprises the following steps:
s1, weighing casein and deionized water, sequentially adding the casein and the deionized water into a reaction container, dropwise adding sodium hydroxide until the pH value of reaction liquid is 9.5-10.5, and fully stirring and completely dissolving to obtain a casein solution;
wherein the concentration of casein in the casein solution is 80 g/L;
s2, adding sophocarpine into an ethanol solution with the mass fraction of 30-70%, and fully stirring and completely dissolving to obtain a sophocarpine solution;
wherein the mass ratio of the sophocarpine to the ethanol solution is 1: 14;
s3, placing the reaction container filled with the casein solution into a water bath at 40-60 ℃, stirring at the speed of 300-500 rpm, continuously dropwise adding the sophocarpine solution, continuously stirring for 2-5 hours after all the sophocarpine solution is dropwise added, and cooling to room temperature to obtain a mixed reaction solution;
wherein the volume ratio of the casein solution to the sophocarpine solution is 1: 1.4;
s4, weighing tea tree oil and dichloromethane, mixing the tea tree oil and the dichloromethane into a reactor, fully stirring and dissolving, and then adding acetic acid to adjust the pH value of the liquid to 4.0-5.0 to obtain a tea tree oil solution;
wherein the mass ratio of the tea tree oil to the dichloromethane is 1: 7;
s5, dropwise adding the mixed reaction liquid into the tea tree oil solution at a stirring speed of 300-500 rpm, continuously adding acetic acid in the dropwise adding process to keep the pH value of the liquid in the reactor at 4.0-5.0, continuously stirring for 1-4 h after all the mixed reaction liquid is added, standing for 5-10 h, centrifugally collecting solid precipitates, washing the precipitates with distilled water for at least three times, and performing freeze drying treatment to obtain tea tree oil/casein modified microspheres;
wherein the volume ratio of the tea tree oil solution to the mixed reaction solution is 1: 2.5.
Example 2
The tea tree oil-containing auditory canal cleanser for pets comprises the following components in parts by weight:
5 parts of tea tree oil/casein modified microspheres, 4 parts of glycerol, 2 parts of cellulose derivatives and 50 parts of deionized water.
The particle size of the tea tree oil/casein modified microspheres is 20-100 mu m.
The cellulose derivative comprises carboxymethyl cellulose.
The preparation method of the casein modified microspheres comprises the following steps:
s1, weighing casein and deionized water, sequentially adding the casein and the deionized water into a reaction container, dropwise adding sodium hydroxide until the pH value of reaction liquid is 9.5-10.5, and fully stirring and completely dissolving to obtain a casein solution;
wherein the concentration of casein in the casein solution is 50 g/L;
s2, adding sophocarpine into an ethanol solution with the mass fraction of 30-70%, and fully stirring and completely dissolving to obtain a sophocarpine solution;
wherein the mass ratio of the sophocarpine to the ethanol solution is 1: 12;
s3, placing the reaction container filled with the casein solution into a water bath at 40-60 ℃, stirring at the speed of 300-500 rpm, continuously dropwise adding the sophocarpine solution, continuously stirring for 2-5 hours after all the sophocarpine solution is dropwise added, and cooling to room temperature to obtain a mixed reaction solution;
wherein the volume ratio of the casein solution to the sophocarpine solution is 1: 1.2;
s4, weighing tea tree oil and dichloromethane, mixing the tea tree oil and the dichloromethane into a reactor, fully stirring and dissolving, and then adding acetic acid to adjust the pH value of the liquid to 4.0-5.0 to obtain a tea tree oil solution;
wherein the mass ratio of the tea tree oil to the dichloromethane is 1: 5;
s5, dropwise adding the mixed reaction liquid into the tea tree oil solution at a stirring speed of 300-500 rpm, continuously adding acetic acid in the dropwise adding process to keep the pH value of the liquid in the reactor at 4.0-5.0, continuously stirring for 1-4 h after all the mixed reaction liquid is added, standing for 5-10 h, centrifugally collecting solid precipitates, washing the precipitates with distilled water for at least three times, and performing freeze drying treatment to obtain tea tree oil/casein modified microspheres;
wherein the volume ratio of the tea tree oil solution to the mixed reaction solution is 1: 2.2.
Example 3
The tea tree oil-containing auditory canal cleanser for pets comprises the following components in parts by weight:
20 parts of tea tree oil/casein modified microspheres, 10 parts of glycerol, 6 parts of cellulose derivatives and 100 parts of deionized water.
The particle size of the tea tree oil/casein modified microspheres is 20-100 mu m.
The cellulose derivative comprises hydroxyethyl cellulose.
The preparation method of the casein modified microspheres comprises the following steps:
s1, weighing casein and deionized water, sequentially adding the casein and the deionized water into a reaction container, dropwise adding sodium hydroxide until the pH value of reaction liquid is 9.5-10.5, and fully stirring and completely dissolving to obtain a casein solution;
wherein the concentration of casein in the casein solution is 100 g/L;
s2, adding sophocarpine into an ethanol solution with the mass fraction of 30-70%, and fully stirring and completely dissolving to obtain a sophocarpine solution;
wherein the mass ratio of the sophocarpine to the ethanol solution is 1: 16;
s3, placing the reaction container filled with the casein solution into a water bath at 40-60 ℃, stirring at the speed of 300-500 rpm, continuously dropwise adding the sophocarpine solution, continuously stirring for 2-5 hours after all the sophocarpine solution is dropwise added, and cooling to room temperature to obtain a mixed reaction solution;
wherein the volume ratio of the casein solution to the sophocarpine solution is 1: 1.6;
s4, weighing tea tree oil and dichloromethane, mixing the tea tree oil and the dichloromethane into a reactor, fully stirring and dissolving, and then adding acetic acid to adjust the pH value of the liquid to 4.0-5.0 to obtain a tea tree oil solution;
wherein the mass ratio of the tea tree oil to the dichloromethane is 1: 8;
s5, dropwise adding the mixed reaction liquid into the tea tree oil solution at a stirring speed of 300-500 rpm, continuously adding acetic acid in the dropwise adding process to keep the pH value of the liquid in the reactor at 4.0-5.0, continuously stirring for 1-4 h after all the mixed reaction liquid is added, standing for 5-10 h, centrifugally collecting solid precipitates, washing the precipitates with distilled water for at least three times, and performing freeze drying treatment to obtain tea tree oil/casein modified microspheres;
wherein the volume ratio of the tea tree oil solution to the mixed reaction solution is 1: 2.8.
Comparative example 1
The auditory canal cleanser for the pet comprises the following components in parts by weight:
16 parts of tea tree oil, 8 parts of glycerol, 4 parts of cellulose derivative and 80 parts of deionized water.
The cellulose derivative is methyl cellulose.
Comparative example 2
The auditory canal cleanser for the pet comprises the following components in parts by weight:
16 parts of tea tree oil/casein composite microspheres, 8 parts of glycerol, 4 parts of cellulose derivatives and 80 parts of deionized water.
The cellulose derivative is methyl cellulose.
The preparation method of the casein composite microspheres comprises the following steps:
s1, weighing casein and deionized water, sequentially adding the casein and the deionized water into a reaction container, dropwise adding sodium hydroxide until the pH value of reaction liquid is 9.5-10.5, and fully stirring and completely dissolving to obtain a casein solution;
wherein the concentration of casein in the casein solution is 80 g/L;
s2, weighing tea tree oil and dichloromethane, mixing the tea tree oil and the dichloromethane into a reactor, fully stirring and dissolving, and then adding acetic acid to adjust the pH value of the liquid to 4.0-5.0 to obtain a tea tree oil solution;
wherein the mass ratio of the tea tree oil to the dichloromethane is 1: 7;
s3, dropwise adding a casein solution into a tea tree oil solution at a stirring speed of 300-500 rpm, continuously adding acetic acid in the dropwise adding process to keep the pH value of liquid in a reactor at 4.0-5.0, continuously stirring for 1-4 h after all the casein solution is added, standing for 5-10 h, centrifuging to collect solid precipitate, washing the precipitate with distilled water for at least three times, and performing freeze drying treatment to obtain tea tree oil/casein composite microspheres;
wherein the volume ratio of the tea tree oil solution to the casein solution is 1: 2.5.
In order to more clearly illustrate the invention, the ear canal cleaners for pets prepared in examples 1 to 3 and comparative examples 1 to 2 of the invention were subjected to performance detection comparison, 15 healthy pet dogs of the same race were selected and randomly divided into 5 groups, the ear canals were cleaned by using the ear canal cleaners for pets prepared in examples 1 to 3 and comparative examples 1 to 2 of the invention, respectively, after cleaning, the inhibition rates of malassezia, staphylococcus aureus, viridans streptococcus and candida albicans in the ear canals were detected every 4 hours for a total of 12 hours, and the detection results are shown in tables 1 to 4.
TABLE 1 inhibition rate of malassezia in the ear canal
TABLE 2 antibacterial Rate of Staphylococcus aureus in the ear canal
TABLE 3 antibacterial Rate of Streptococcus viridis in the auditory canal
TABLE 4 bacteriostasis rate of Candida albicans in the ear canal
As can be seen from tables 1 to 4, although the ear canal cleanser prepared in comparative example 1 has a high antibacterial rate immediately after cleaning, the antibacterial rate is reduced by 8% to 13% after 12 hours of cleaning, the antibacterial rate of the ear canal cleanser prepared in comparative example 2 is similar to that of the ear canal cleanser prepared in the embodiment of the present invention, but the antibacterial rate is reduced by 13% to 16% after 12 hours of cleaning, whereas examples 1 to 3 of the present invention have a high antibacterial rate immediately after cleaning, and the antibacterial rate is reduced only to a small extent after 12 hours, and the reduction rate is lower than 5%, which indicates that the ear canal cleanser prepared in examples 1 to 3 of the present invention has the advantages of good antibacterial effect and long-term antibacterial effect.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (6)
1. The tea tree oil-containing ear canal cleanser for pets is characterized by comprising the following components in parts by weight:
5-20 parts of tea tree oil/casein modified microspheres, 4-10 parts of glycerol, 2-6 parts of cellulose derivatives and 50-100 parts of deionized water.
2. The tea tree oil-containing ear canal cleanser for pets according to claim 1, wherein the ear canal cleanser comprises the following components in parts by weight:
8-15 parts of tea tree oil/casein modified microspheres, 5-8 parts of glycerol, 3-5 parts of cellulose derivatives and 65-80 parts of deionized water.
3. The tea tree oil-containing ear canal cleanser for pets according to claim 1, wherein the ear canal cleanser comprises the following components in parts by weight:
5-20 parts of tea tree oil/casein modified microspheres, 4-10 parts of glycerol, 2-6 parts of cellulose derivatives and 50-100 parts of deionized water.
4. The tea tree oil-containing ear canal cleanser for pets according to claim 1, wherein the particle size of the tea tree oil/casein-modified microspheres is 20 to 100 μm.
5. The pet ear canal cleanser containing tea tree oil as claimed in claim 1, wherein said cellulose derivative comprises at least one of methyl cellulose, carboxymethyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose.
6. The auditory canal cleanser for pets containing tea tree oil according to claim 1, wherein the casein-modified microspheres are prepared by the following steps:
s1, weighing casein and deionized water, sequentially adding the casein and the deionized water into a reaction container, dropwise adding sodium hydroxide until the pH value of reaction liquid is 9.5-10.5, and fully stirring and completely dissolving to obtain a casein solution;
wherein the concentration of casein in the casein solution is 50-100 g/L;
s2, adding sophocarpine into an ethanol solution with the mass fraction of 30-70%, and fully stirring and completely dissolving to obtain a sophocarpine solution;
wherein the mass ratio of the sophocarpine to the ethanol solution is 1: 12-16;
s3, placing the reaction container filled with the casein solution into a water bath at 40-60 ℃, stirring at the speed of 300-500 rpm, continuously dropwise adding the sophocarpine solution, continuously stirring for 2-5 hours after all the sophocarpine solution is dropwise added, and cooling to room temperature to obtain a mixed reaction solution;
wherein the volume ratio of the casein solution to the sophocarpine solution is 1: 1.2-1.6;
s4, weighing tea tree oil and dichloromethane, mixing the tea tree oil and the dichloromethane into a reactor, fully stirring and dissolving, and then adding acetic acid to adjust the pH value of the liquid to 4.0-5.0 to obtain a tea tree oil solution;
wherein the mass ratio of the tea tree oil to the dichloromethane is 1: 5-8;
s5, dropwise adding the mixed reaction liquid into the tea tree oil solution at a stirring speed of 300-500 rpm, continuously adding acetic acid in the dropwise adding process to keep the pH value of the liquid in the reactor at 4.0-5.0, continuously stirring for 1-4 h after all the mixed reaction liquid is added, standing for 5-10 h, centrifugally collecting solid precipitates, washing the precipitates with distilled water for at least three times, and performing freeze drying treatment to obtain tea tree oil/casein modified microspheres;
wherein the volume ratio of the tea tree oil solution to the mixed reaction liquid is 1: 2.2-2.8.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114306179A (en) * | 2021-12-01 | 2022-04-12 | 佛山市思怡诺生物科技有限公司 | External prevention and treatment nursing agent for pet skin and ears |
-
2021
- 2021-05-10 CN CN202110507319.3A patent/CN113208950A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114306179A (en) * | 2021-12-01 | 2022-04-12 | 佛山市思怡诺生物科技有限公司 | External prevention and treatment nursing agent for pet skin and ears |
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