CN113203611A - Clinical laboratory is with getting phlegm cup and phlegm sample preprocessing device - Google Patents

Clinical laboratory is with getting phlegm cup and phlegm sample preprocessing device Download PDF

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Publication number
CN113203611A
CN113203611A CN202110470875.8A CN202110470875A CN113203611A CN 113203611 A CN113203611 A CN 113203611A CN 202110470875 A CN202110470875 A CN 202110470875A CN 113203611 A CN113203611 A CN 113203611A
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cup
sputum
cavity
phlegm
taking
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CN113203611B (en
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陈璐璐
吴玉丹
丁雪贞
张永江
姜雅琳
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First Affiliated Hospital of Henan University of Science and Technology
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First Affiliated Hospital of Henan University of Science and Technology
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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Abstract

The utility model provides a clinical laboratory is with getting phlegm cup and phlegm sample preprocessing device, includes the casing and sets up a plurality of phlegm cup of getting that are in the casing and place the seat, each gets and has the chamber that holds that puts and get the phlegm cup and to getting into compound enzymolysis liquid in the phlegm cup of putting to carry out the syringe of preliminary treatment to the phlegm sample of getting in the phlegm cup, get the phlegm sample through preliminary treatment in the phlegm cup, fall into the bacterial culture mechanism that the pre-installation has the culture solution through the honeycomb duct of its bottom and cultivate the propagation. The invention can process the sputum sample, so that bacteria in the sputum sample can be propagated in the queuing inspection process and can be detected more easily, thereby greatly shortening the detection time of the sputum sample, improving the detection efficiency, and more importantly, more accurately detecting the trace pathogenic bacteria contained in the sputum sample.

Description

Clinical laboratory is with getting phlegm cup and phlegm sample preprocessing device
Technical Field
The invention relates to the field of sputum sample inspection in the medical field, in particular to a sputum taking cup and a sputum sample pretreatment device for a clinical laboratory.
Background
Sputum is the secretion of alveoli, bronchi and trachea, and the amount of sputum is very small in healthy people. In pathological conditions, after respiratory mucosa and alveoli are stimulated, the mucous membranes are congested and edematous, serous fluid is exuded, mucus secretion is increased, and exudates such as various cells and fibrin are mixed with mucus, inhaled dust, certain tissue necrotic matters and the like to form sputum. The composition of sputum is complex and consists of 95% moisture and 5% dust, protein, etc.
The sputum examination has certain value for diagnosis, observation of curative effect and prognosis of respiratory system diseases such as pulmonary tuberculosis, lung fluke, lung tumor, bronchial asthma, chronic bronchitis, bronchiectasis and the like.
During sputum examination, a sputum sample of a patient is generally extracted and then sent to a clinical laboratory, and the types of bacteria in the sputum are analyzed by an instrument so as to judge the types of diseases; however, in hospitals, since there are too many patients to be examined, it takes a long time to queue up a sputum sample for examination after delivery, and therefore, it is generally necessary to temporarily store the sputum sample in a refrigerated state and then conduct examination after treatment, which results in a prolonged examination time.
Disclosure of Invention
In order to solve the problem that the existing sputum sample is prolonged in inspection time after being refrigerated and stored, the invention provides a sputum taking cup and a sputum sample pretreatment device for a clinical laboratory, wherein the pretreatment device can be used for treating the sputum sample, so that bacteria in the sputum sample are propagated in a queuing inspection process and are more easily detected, the detection time of the sputum sample is greatly shortened, the detection efficiency is improved, and more importantly, the micro pathogenic bacteria in the sputum sample can be more accurately detected.
The technical scheme adopted by the invention for solving the technical problems is as follows: a sputum taking cup for a clinical laboratory is provided with a conical cup body, a sealing cover is arranged at the top of the cup body, a flow guide pipe is arranged at the bottom of the cup body, an opening at the bottom end of the flow guide pipe is sealed by a plugging cap, and a filtering assembly is arranged in the flow guide pipe; the side wall of the upper part of the cup body is provided with a disposable injection hole which can be punctured by external force.
Use above-mentioned phlegm sample preprocessing device who gets phlegm cup, including casing and a plurality of phlegm cup of getting of setting in the casing seat of placing, each gets and has the chamber that holds of placing the phlegm cup and to getting into compound enzymolysis liquid in the phlegm cup place the seat to carry out the syringe of preliminary treatment to the phlegm sample of getting in the phlegm cup, get the phlegm sample through preliminary treatment in the phlegm cup, fall into the bacterial culture mechanism that the pre-installation has the culture solution through the honeycomb duct of its bottom and cultivate the propagation extension.
As an optimized scheme of the sputum sample pretreatment device, the shell is internally divided into a sputum cup placing cavity, a culture dish taking and placing cavity and a culture solution storage cavity from top to bottom, wherein the sputum cup placing seat is arranged in the sputum cup placing cavity, the top of the sputum cup placing cavity is provided with an opening which can be opened and is sealed by a cover plate, and the sputum cup is placed in or taken out through the opening; the bacterial culture mechanism is arranged in the culture dish taking and placing cavity, and the side wall of the culture dish taking and placing cavity is provided with an openable window so as to take out or place the culture dish pre-filled with culture solution in the bacterial culture mechanism through the window; through holes which are in one-to-one correspondence with the sputum taking cups are distributed at the top of the culture dish taking and placing cavity, and when the sputum taking cups are placed in the sputum taking cup placing cavity, the flow guide pipes on the sputum taking cups penetrate through the through holes to extend into the culture dish taking and placing cavity, and the preprocessed sputum sample in each sputum taking cup is dripped into a culture dish of a bacterial culture mechanism; the culture solution storage cavity is used for storing culture solution, and the culture solution is conveyed to each culture dish through the pumping mechanism.
As another kind of optimization scheme of above-mentioned phlegm sample preprocessing device, get a plurality of rows of window direction regular distribution that phlegm cup arrangement seat got the chamber along the culture dish in the casing, the culture dish that each row got phlegm cup arrangement seat below passes through the connecting piece to connect as an organic wholely, and extend the window through handle portion, be convenient for pull out or push the culture dish through handle portion, the both sides of culture dish have along the track that the culture dish was taken out or was pushed the direction, be provided with the heat preservation chamber in the below of culture dish for keep the temperature in the culture dish.
As another optimization scheme of the sputum sample pretreatment device, the top of the culture dish taking and placing cavity is provided with a plurality of strip liquid distribution cavities, each strip liquid distribution cavity corresponds to one row of culture dishes below, the bottom of each strip liquid distribution cavity is provided with a plurality of culture liquid discharge pipes for injecting culture liquid in the strip liquid distribution cavity into each culture dish, the pumping mechanism comprises a plurality of micro liquid pumps, each micro liquid pump corresponds to one strip liquid distribution cavity, and a liquid suction pipe of each micro liquid pump extends into the culture liquid storage cavity to extract the culture liquid and pumps the culture liquid into the strip liquid distribution cavities.
As another optimization scheme of the sputum sample pretreatment device, a supporting piece surrounding a through hole is arranged in the containing cavity, the supporting piece is of a cavity structure, the outer side wall of the supporting piece is provided with a reverse truncated cone-shaped limiting cylinder, and the limiting cylinder is matched with the outer wall of the sputum taking cup to realize positioning of the sputum taking cup.
As another optimized scheme of the sputum sample pretreatment device, the sputum taking cup placing seat comprises a limiting pedestal, and the limiting pedestal is matched with a limiting pedestal of another sputum taking cup placing seat to form an accommodating cavity; the injector comprises a compound enzymatic hydrolysate storage cavity and a piston cavity, wherein the compound enzymatic hydrolysate storage cavity is arranged in the limiting pedestal, the piston cavity is provided with a piston, and the bottom of the piston cavity is communicated with the compound enzymatic hydrolysate storage cavity through a pipette with a one-way valve so as to suck the compound enzymatic hydrolysate in the compound enzymatic hydrolysate storage cavity into the piston cavity when the piston is drawn out; bottom one side in piston chamber is provided with the protrusion in the fluid-discharge tube that holds the chamber lateral wall, and when getting the sputum cup and arranging the in-process that holds the chamber in, the tip of fluid-discharge tube pierces through in the disposable injection hole on the cup stretches into the cup to when pushing down the piston, in impressing the compound enzyme solution of piston intracavity into the cup.
As another optimization scheme of the sputum sample pretreatment device, a clamping joint is formed after the top end of the piston extends out of the piston cavity, the clamping joint is in butt joint with a hole in the extension part of the upper sealing cover of the cup body, when the sputum taking cup is placed into the containing cavity, the extension part drives the clamping joint to push the piston downwards, complex enzymolysis liquid in the piston cavity is extruded into the cup body, and the complex enzymolysis liquid is mixed with the sputum sample for pretreatment.
As another optimization scheme of the sputum sample pretreatment device, the top end of the piston cavity is provided with a sealing plug.
As another optimization scheme of the sputum sample pretreatment device, the free end of the liquid discharge pipe is detachably connected with a disposable puncture tube, and the disposable puncture tube punctures a disposable injection hole and enters the cup body in the process of putting the sputum taking cup into the accommodating cavity.
Compared with the prior art, the invention has the following beneficial effects:
1) the sputum taking cup is provided with the disposable injection hole, so that the composite enzymatic hydrolysate for treating the sputum sample can be injected into the sputum taking cup in a closed state with the sputum sample, and meanwhile, the filtering component of the bottom diversion pipe can enable the pretreated sputum sample to flow out and filter impurities such as floating dust in the outflow process;
2) the sputum sample pretreatment device can treat the sputum sample, so that bacteria in the sputum sample can be propagated in the queuing inspection process and can be detected more easily, the detection time of the sputum sample is greatly shortened, the detection efficiency is improved, and more importantly, trace pathogenic bacteria in the sputum sample can be detected more accurately;
3) the sputum sample pretreatment device can be matched with the sputum taking cup to treat a plurality of sputum samples at one time, and can automatically realize the injection of compound enzymatic hydrolysate in the process of putting the sputum taking cup, and automatically fall into a culture dish filled with culture solution for propagation after reacting for a period of time when the sputum samples become thin, thereby shortening the treatment and culture time during detection, and enabling trace pathogenic bacteria possibly contained in the sputum samples to propagate and be more easily detected.
Drawings
FIG. 1 is a schematic view of a sputum sample pretreatment apparatus of the present invention in use;
FIG. 2 is a schematic view of a sputum sample pretreatment apparatus according to the present invention;
FIG. 3 is a schematic diagram showing the arrangement of the sputum cup holder in the sputum sample pretreatment apparatus;
FIG. 4 is a schematic view of the structure of the sputum cup;
FIG. 5 is a schematic view of the sputum cup holder with the syringe not depressed;
FIG. 6 is a schematic view of the injector being pressed down in the sputum cup holder;
FIG. 7 is a schematic view of the connection of the culture dish;
reference numerals: 1. a shell, 101, a sputum cup placing cavity, 102, a culture dish taking and placing cavity, 103, a culture solution storage cavity, 104, a cover plate, 2, a sputum cup, 201, a cup body, 202, a guide pipe, 203, a filtering component, 204, a plugging cap, 205, a sealing cover, 206, a disposable injection hole, 207, an extension part, 3, a sputum cup placing seat, 301, a limiting seat, 302, a containing cavity, 303, a through hole, 304, a limiting cylinder, 305, a supporting piece, 4, a bacteria culture mechanism, 401, a culture dish, 402, a track, 403, a heat preservation cavity, 404, a connecting piece, 405, a handle part, 5, an injector, 501, a piston cavity, 502, a piston, 503, a clamping head, 504, a liquid discharge pipe, 505, a disposable puncture tube, 506, a sealing plug, 507, a complex enzyme, 508, a complex enzyme solution storage cavity, 6, a pumping mechanism, 601, a micro liquid pump, 602, a liquid pumping tube, 603, a strip liquid distribution tube, 604, a strip liquid distribution tube, a strip-shaped liquid distribution tube, a filter, And a culture solution discharge pipe.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following specific embodiments. The invention is not illustrated in the following examples, which are prior art.
Example 1
As shown in fig. 4, a sputum-taking cup for clinical laboratory, the sputum-taking cup 2 has a conical cup body 201, a sealing cover 205 is arranged on the top of the cup body 201, a flow guide pipe 202 is arranged on the bottom, the bottom end opening of the flow guide pipe 202 is sealed by a sealing cap 204, a filter component 203 is arranged in the flow guide pipe 202, and the filter component 203 is a filter cotton yarn; the upper side wall of the cup body 201 is provided with a disposable injection hole 206 which can be punctured by external force.
In this embodiment, the sputum cup 2 is a disposable plastic cup made of medical plastic, and the sealing cap 205 on the top is sealed after the sputum sample is filled with the bacteria to escape into the air; because the sputum is generally more viscous, the existence of the filtering component 203 in the flow guide pipe 202 can make the sputum sample stay on the filtering component 203; the plugging cap 204 with an opening at the bottom end of the flow guide pipe 202 is also used for preventing phlegm samples from seeping out, and can be fixed at the bottom of the flow guide pipe 202 in an adhesion or other detachable mode; the disposable injection hole 206 on the upper side wall of the cup body 201 is intact when taking a sputum sample, so that a closed space is formed by matching the plugging cap 204 and the sealing cover 205, and germs in the sputum sample are prevented from escaping into the air; the disposable injection hole 206 is easily punctured to facilitate injection of an enzymatic solution thereto for pretreatment of the sputum sample.
Example 2
As shown in fig. 1-3, the sputum sample pretreatment device using the sputum cup extraction described in embodiment 1 includes a housing 1 and a plurality of sputum cup placement seats 3 disposed in the housing 1, each of the sputum cup placement seats 3 has a containing cavity 302 for placing the sputum cup 2 and injects a compound enzymatic hydrolysate into the sputum cup 2, so as to pretreat the sputum sample in the sputum cup 2, and the pretreated sputum sample in the sputum cup 2 falls into a bacteria culture mechanism 4 pre-filled with a culture solution through a flow guide pipe 202 at the bottom of the sputum cup for propagation.
Used compound enzymatic hydrolysate in this embodiment, its composition is the compound enzymatic hydrolysate that current clinical laboratory used, its main aim at, in advance with the proteolysis of protein in the phlegm sample, make the phlegm sample break away from and glue thick state, the germ that wherein contains can flow into bacterium culture mechanism 4 along with the phlegm sample that becomes liquid, owing to be equipped with filter assembly 203 in the honeycomb duct 202 of getting phlegm cup 2, it can be to the further filtration of impurity such as dust in the phlegm sample, make the bacterium instil into in bacterium culture mechanism 4, and expand the reproduction in the culture solution, the trace pathogenic bacterium of being convenient for can be detected out more easily.
The foregoing is a basic embodiment of the present invention, and further modifications, optimizations and limitations can be made on the foregoing, so as to obtain the following examples:
example 3
The present embodiment is an optimized scheme based on embodiment 2, and the main structure of the present embodiment is the same as that of embodiment 2, and the improvement point is that: as shown in fig. 1-2, the inside of the housing 1 is divided into a sputum cup placing cavity 101, a culture dish taking and placing cavity 102 and a culture solution storage cavity 103 from top to bottom, wherein the sputum cup placing seat 3 is arranged in the sputum cup placing cavity 101, an openable cover plate 104 is arranged at the top of the sputum cup placing cavity 101 for sealing, and the sputum cup 2 is placed in or taken out through the opening; the bacterial culture mechanism 4 is arranged in the culture dish taking and placing cavity 102, and the side wall of the culture dish taking and placing cavity 102 is provided with an openable window so as to take out or place a culture dish 401, which is pre-filled with culture solution, from the bacterial culture mechanism 4 through the window; through holes 303 corresponding to the sputum taking cups 2 one by one are distributed on the top of the culture dish taking and placing cavity 102, when the sputum taking cups 2 are placed in the sputum taking cup placing cavity 101, the flow guide pipes 202 on the sputum taking cups penetrate through the through holes 303 and extend into the culture dish taking and placing cavity 102, and pretreated sputum samples in each sputum taking cup 2 are dripped into a culture dish 401 of one bacteria culture mechanism 4; the culture solution storage cavity 103 stores culture solution, and the culture solution is conveyed to each culture dish 401 through the pumping mechanism 6.
Example 4
The present embodiment is an optimized scheme based on embodiment 3, and the main structure of the present embodiment is the same as that of embodiment 3, and the improvement point is that: as shown in fig. 3, 5, 6 and 7, a plurality of rows of sputum-taking cup holders 3 in the housing 1 are regularly distributed along the direction of the window of the culture dish taking and placing cavity 102, the culture dishes 401 below each row of sputum-taking cup holders 3 are connected into a whole through a connecting piece 404, and extend out of the window through a handle part 405, so that the culture dishes 401 can be pulled out or pushed in through the handle part 405, two sides of the culture dishes 401 are provided with tracks 402 along the direction of pulling out or pushing in the culture dishes 401, and a heat preservation cavity 403 is arranged below the culture dishes 401 for preserving the temperature in the culture dishes 401.
In this embodiment, the heat preservation chamber 403 has a constant temperature heating wire therein; the culture dish 401 is internally sleeved with a disposable plastic culture dish, thereby being convenient for replacement.
Example 5
The present embodiment is an optimized scheme based on embodiment 4, and the main structure of the present embodiment is the same as that of embodiment 4, and the improvement point is that: as shown in fig. 5 and 6, the top of the culture dish taking and placing cavity 102 has a plurality of strip-shaped liquid distribution cavities 603, each strip-shaped liquid distribution cavity 603 corresponds to one row of culture dishes 401 below, the bottom of the strip-shaped liquid distribution cavity 603 is provided with a plurality of culture liquid discharge pipes 604 for injecting the culture liquid in the strip-shaped liquid distribution cavity 603 into each culture dish 401, the pumping mechanism 6 comprises a plurality of micro liquid pumps 601, each micro liquid pump 601 corresponds to one strip-shaped liquid distribution cavity 603, and the liquid suction pipe 602 of the micro liquid pump 601 extends into the culture liquid storage cavity 103 to draw the culture liquid and pumps the culture liquid into the strip-shaped liquid distribution cavity 603.
Example 6
The present embodiment is another optimization scheme based on embodiment 2, and the main structure of the present embodiment is the same as that of embodiment 2, and the improvement point is that: as shown in fig. 5 and 6, a supporting member 305 surrounding the through hole 303 is arranged in the accommodating cavity 302, the inside of the supporting member 305 is a cavity structure, an inverted truncated cone-shaped limiting barrel 304 is arranged on the outer side wall of the supporting member 305, and the limiting barrel 304 is matched with the outer wall of the sputum taking cup 2 to position the sputum taking cup 2.
Example 7
The present embodiment is another optimization scheme based on embodiment 2, and the main structure of the present embodiment is the same as that of embodiment 2, and the improvement point is that: as shown in fig. 5 and 6, the sputum cup placing seat 3 comprises a limiting seat 301, and the limiting seat 301 is matched with the limiting seat 301 of another sputum cup placing seat 3 to form an accommodating cavity 302; the injector 5 comprises a compound enzymatic hydrolysate storage cavity 508 arranged in the limiting pedestal 301 and a piston cavity 501 with a piston 502, wherein the bottom of the piston cavity 501 is communicated with the compound enzymatic hydrolysate storage cavity 508 through a pipette 507 with a one-way valve, so that when the piston 502 is drawn out, the compound enzymatic hydrolysate in the compound enzymatic hydrolysate storage cavity 508 is sucked into the piston cavity 501; a liquid discharge pipe 504 protruding out of the side wall of the accommodating cavity 302 is arranged on one side of the bottom of the piston cavity 501, and when the sputum taking cup 2 is placed in the accommodating cavity 302, the end of the liquid discharge pipe 504 pierces the disposable injection hole 206 on the cup body 201 and extends into the cup body 201, and when the piston 502 is pressed down, the composite enzymolysis liquid in the piston cavity 501 is pressed into the cup body 201.
Example 8
This embodiment is an optimized solution based on embodiment 7, and the main structure thereof is the same as that of embodiment 7, and the improvement point is that: as shown in fig. 5 and 6, the top end of the piston 502 extends out of the piston cavity 501 to form a snap joint 503, the snap joint 503 is in butt joint with a hole on the extension 207 of the sealing cover 205 on the cup body 201, and when the sputum taking cup 2 is placed into the accommodating cavity 302, the extension 207 drives the snap joint 503 to push the piston 502 downwards, so that the composite enzymatic hydrolysate in the piston cavity 501 is squeezed into the cup body 201, and is mixed with the sputum sample for pretreatment.
Example 9
The present embodiment is another optimization scheme based on embodiment 7, and the main structure thereof is the same as embodiment 7, and the improvement point is that: as shown in fig. 5 and 6, the top end of the piston chamber 501 is provided with a sealing plug 506.
Example 10
The present embodiment is another optimization scheme based on embodiment 7, and the main structure thereof is the same as embodiment 7, and the improvement point is that: as shown in figures 5 and 6, a disposable puncture tube 505 is detachably connected to the free end of the drainage tube 504, and the disposable puncture tube 505 punctures the disposable injection hole 206 and enters the cup body 201 during the process of placing the sputum cup 2 into the containing cavity 302.
In this embodiment, the end of the disposable puncture tube 505 is a slant surface and gradually retracts from the upper edge to the lower edge of the end, so that the upper edge pierces the sealing cap of the disposable injection hole 206 and a gap is formed between the lower edge and the sealing cap, thereby facilitating the composite enzymatic hydrolysate to flow into the cup body 201.

Claims (10)

1. The utility model provides a clinical laboratory is with getting phlegm cup which characterized in that: the sputum taking cup (2) is provided with a conical cup body (201), the top of the cup body (201) is provided with a sealing cover (205), the bottom of the cup body is provided with a flow guide pipe (202), the bottom end opening of the flow guide pipe (202) is sealed by a plugging cap (204), and a filtering component (203) is arranged in the flow guide pipe (202); the side wall of the upper part of the cup body (201) is provided with a disposable injection hole (206) which can be punctured by external force.
2. A sputum sample pretreatment apparatus using the sputum cup according to claim 1, characterized in that: including casing (1) and set up a plurality of phlegm cup of getting seat (3) in casing (1), each gets and has the chamber (302) that holds that puts and get phlegm cup (2) and inject compound enzymolysis liquid in getting phlegm cup (2) in phlegm cup seat (3) to carry out syringe (5) of preliminary treatment to the phlegm sample that gets in the phlegm cup (2), get the phlegm sample through preliminary treatment in phlegm cup (2), fall into through honeycomb duct (202) of its bottom and cultivate the propagation in the bacterial culture mechanism (4) that the pre-installation has the culture solution.
3. The sputum sample pretreatment device of claim 2, wherein: the interior of the shell (1) is divided into a sputum taking cup placing cavity (101), a culture dish taking and placing cavity (102) and a culture solution storage cavity (103) from top to bottom, wherein the sputum taking cup placing seat (3) is arranged in the sputum taking cup placing cavity (101), the top of the sputum taking cup placing cavity (101) is provided with an opening which can be opened and is sealed by a cover plate (104), and the sputum taking cup (2) is placed in or taken out through the opening; the bacterial culture mechanism (4) is arranged in the culture dish taking and placing cavity (102), and the side wall of the culture dish taking and placing cavity (102) is provided with an openable window so as to take out or place a culture dish (401) pre-filled with culture solution in the bacterial culture mechanism (4) through the window; through holes (303) which are in one-to-one correspondence with the sputum taking cups (2) are distributed at the top of the culture dish taking and placing cavity (102), when the sputum taking cups (2) are placed in the sputum taking cup placing cavity (101), the guide pipes (202) on the sputum taking cups penetrate through the through holes (303) and extend into the culture dish taking and placing cavity (102), and the preprocessed sputum sample in each sputum taking cup (2) is dripped into a culture dish (401) of a bacteria culture mechanism (4); the culture solution storage cavity (103) stores culture solution, and the culture solution is conveyed to each culture dish (401) through a pumping mechanism (6).
4. The sputum sample pretreatment device of claim 3, wherein: the utility model discloses a sputum collection device, including casing (1), get the window direction law distribution a plurality of rows that phlegm cup mount pad (3) were put along the culture dish in casing (1), culture dish (401) of every row of phlegm cup mount pad (3) below are connected as an organic wholely through connecting piece (404), and extend the window through handle portion (405), be convenient for pull out or push culture dish (401) through handle portion (405), the both sides of culture dish (401) have along track (402) of culture dish (401) extraction or push-in direction, below in culture dish (401) is provided with heat preservation chamber (403), a temperature for in keeping culture dish (401).
5. The sputum sample pretreatment device of claim 4, wherein: the top that the chamber (102) was got to the culture dish has a plurality of strip cloth liquid chamber (603), and each strip cloth liquid chamber (603) corresponds with one row of culture dish (401) of below, and strip cloth liquid chamber (603) bottom sets up a plurality of culture solution discharge pipe (604) of pouring into each culture dish (401) with the culture solution in strip cloth liquid chamber (603), pumping mechanism (6) include a plurality of miniature fluid pump (601), every miniature fluid pump (601) correspond a strip cloth liquid chamber (603), and draw tube (602) of miniature fluid pump (601) stretch into extract culture solution in culture solution storage chamber (103) to the pumping is to in strip cloth liquid chamber (603).
6. The sputum sample pretreatment device of claim 2, wherein: the receiving cavity (302) is internally provided with a supporting piece (305) surrounding the through hole (303), the supporting piece (305) is internally provided with a cavity structure, the outer side wall of the supporting piece (305) is provided with a reverse truncated cone-shaped limiting barrel (304), and the limiting barrel (304) is matched with the outer wall of the sputum taking cup (2) to realize the positioning of the sputum taking cup (2).
7. The sputum sample pretreatment device of claim 2, wherein: the sputum taking cup placing seat (3) comprises a limiting seat (301), and the limiting seat (301) is matched with the limiting seat (301) of the other sputum taking cup placing seat (3) to form an accommodating cavity (302); the injector (5) comprises a composite enzymatic hydrolysate storage cavity (508) arranged in the limiting pedestal (301) and a piston cavity (501) with a piston (502), wherein the bottom of the piston cavity (501) is communicated with the composite enzymatic hydrolysate storage cavity (508) through a pipette (507) with a one-way valve, so that when the piston (502) is drawn out, the composite enzymatic hydrolysate in the composite enzymatic hydrolysate storage cavity (508) is sucked into the piston cavity (501); a liquid discharge pipe (504) protruding out of the side wall of the accommodating cavity (302) is arranged on one side of the bottom of the piston cavity (501), and when the sputum taking cup (2) is placed in the accommodating cavity (302), the end of the liquid discharge pipe (504) pierces through the disposable injection hole (206) in the cup body (201) and extends into the cup body (201), and when the piston (502) is pressed down, the composite enzymolysis liquid in the piston cavity (501) is pressed into the cup body (201).
8. The sputum sample pretreatment device of claim 7, wherein: the top end of the piston (502) extends out of the piston cavity (501) to form a clamping joint (503), the clamping joint (503) is in butt joint with a hole in an extending part (207) of a sealing cover (205) on the cup body (201), and when the sputum taking cup (2) is placed into the accommodating cavity (302), the extending part (207) drives the clamping joint (503) to push the piston (502) downwards, so that the composite enzymatic hydrolysate in the piston cavity (501) is extruded into the cup body (201), and is mixed with a sputum sample to be pretreated.
9. The sputum sample pretreatment device of claim 7, wherein: the top end of the piston cavity (501) is provided with a sealing plug (506).
10. The sputum sample pretreatment device of claim 7, wherein: the free end of the liquid discharge pipe (504) is detachably connected with a disposable puncture tube (505), and the disposable puncture tube (505) punctures the disposable injection hole (206) and enters the cup body (201) in the process of putting the sputum taking cup (2) into the accommodating cavity (302).
CN202110470875.8A 2021-04-29 2021-04-29 Clinical laboratory is with getting phlegm cup and phlegm sample preprocessing device Active CN113203611B (en)

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