CN113201477A - Production process of hay compound culture medium - Google Patents
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- CN113201477A CN113201477A CN202110504192.XA CN202110504192A CN113201477A CN 113201477 A CN113201477 A CN 113201477A CN 202110504192 A CN202110504192 A CN 202110504192A CN 113201477 A CN113201477 A CN 113201477A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention relates to the technical field of culture medium production, and discloses a production process of a hay compound culture medium, which comprises the following steps: A. weighing the following raw materials in parts by weight: 2-6 parts of corn flour, 0.05-0.4 part of sodium hydroxide, 2-8 parts of hawthorn liquid, 5-8 parts of sweet potato, 3-8 parts of dried orange peel, 190 parts of deionized water, 3-8 parts of coptis powder, 2-6 parts of orange peel, 5-10 parts of glycerol, 1-5 parts of amino acid, 5-10 parts of sugar, 0.5-1.5 parts of potassium chloride and 0.2-0.8 part of potassium dihydrogen phosphate. The process is simple and convenient to operate, production conditions are easy to control, production cost is low, various raw material components can be finely managed and controlled, the amount of the produced bacillus subtilis culture medium is high, the production cost of bacillus subtilis is effectively reduced, the content of pollutants generated in the production process is low, the pollution degree to the environment is small, and the obtained bacillus subtilis has high antibacterial performance and stability and has certain market popularization prospect and practical application value.
Description
Technical Field
The invention relates to the technical field of culture medium production, in particular to a production process of a hay compound culture medium.
Background
The culture medium is an artificially prepared nutrient for growth and maintenance of microorganisms, plant tissues and animal tissues, generally contains carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, water and the like, different culture media can be added with compounds which cannot be synthesized per se, namely growth factors, and some microorganisms such as autotrophic microorganisms do not need carbon sources according to actual requirements, so that the substances only have generality, and the bacillus subtilis is beneficial microorganisms, and at present, the culture medium has a better application effect in numerous fields.
However, the hay culture medium prepared by the existing process generally has the defects of low spore yield and high cost, so that the problems of yield reduction and the like are caused, in the whole production process, various raw material components cannot be finely managed and controlled, the quality of the prepared hay culture medium is easily not up to the standard, and the existing hay culture medium contains a large amount of pollutants, so that certain pollution is caused to the surrounding environment. Therefore, the skilled person provides a production process of a hay compound culture medium to solve the problems in the background art.
Disclosure of Invention
The invention aims to provide a production process of a hay compound culture medium, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a production process of a hay compound culture medium comprises the following steps:
A. weighing the following raw materials in parts by weight: 2-6 parts of corn flour, 0.05-0.4 part of sodium hydroxide, 2-8 parts of hawthorn liquid, 5-8 parts of sweet potato, 3-8 parts of dried orange peel, 190 parts of deionized water, 3-8 parts of coptis powder, 2-6 parts of orange peel, 5-10 parts of glycerol, 1-5 parts of amino acid, 5-10 parts of sugar, 0.5-1.5 parts of potassium chloride and 0.2-0.8 part of potassium dihydrogen phosphate;
B. taking pericarpium citri reticulatae, sugarcane and corn flour according to a ratio, adding deionized water, mixing, soaking for 1.2-1.8h, steaming at high temperature for 25 minutes, taking out, filtering filtrate through a double-layer metal filter screen, and preserving heat to obtain a mixed solution I;
C. taking hawthorn liquid and coptis chinensis powder according to a ratio, grinding orange peel into coarse powder, filtering the coarse powder to obtain fine powder, mixing the fine powder with the hawthorn liquid and the coptis chinensis powder, and then adding sodium hydroxide to adjust the pH value to obtain a mixed solution II;
D. and pouring sugar, glycerol and potassium chloride into the mixed solution I for uniform mixing, pouring amino acid and potassium dihydrogen phosphate into the mixed solution II for uniform mixing, then mixing the mixed solution I and the mixed solution II together, and sterilizing for 11 minutes under the conditions of 102-108 ℃ and 105-115kPa to obtain the hay compound culture medium.
As a still further scheme of the invention: the pH value is 7.2, and after the pH value is adjusted, the mixed solution II is stirred for 35min at the temperature of 82-88 ℃.
As a still further scheme of the invention: grinding the citrus peel into coarse powder, filtering the coarse powder with a filter membrane of 0.21-0.26 μm, and filtering to obtain fine powder.
As a still further scheme of the invention: the sugar is any one of sucrose, glucose, fructose, galactose, lactose or maltose.
As a still further scheme of the invention: the amino acid is any one of alanine, valine, leucine, isoleucine, methionine, proline, tryptophan or serine.
As a still further scheme of the invention: and mixing the mixed solution I and the mixed solution II together, sterilizing, sealing and packaging by using a plastic bag, stacking the sealed and packaged finished product in a storehouse at the temperature of 22-28 ℃, and taking out after anaerobic fermentation.
As a still further scheme of the invention: the anaerobic fermentation time is 30-40 h.
As a still further scheme of the invention: the preparation method of the hawthorn juice comprises the following steps: removing core of fructus crataegi, decocting in water for 25min, taking out, and filtering twice with filter cloth to obtain fructus crataegi solution.
As a still further scheme of the invention: the cooking temperature of the hawthorn is 80-100 ℃, and foam floating on the water surface is fished out during cooking.
As a still further scheme of the invention: the heat preservation temperature of the mixed solution I is 31-35 ℃, and the mixed solution I is continuously stirred and activated for 1.2-1.8h during heat preservation.
Compared with the prior art, the invention has the beneficial effects that: the process is simple and convenient to operate, production conditions are easy to control, production cost is low, various raw material components can be finely managed and controlled, the amount of the produced bacillus subtilis culture medium is high, the production cost of bacillus subtilis is effectively reduced, the content of pollutants generated in the production process is low, the pollution degree to the environment is small, and the obtained bacillus subtilis has high antibacterial performance and stability and has certain market popularization prospect and practical application value.
Detailed Description
In embodiment 1 of the invention, a production process of a hay compound culture medium comprises the following steps:
A. weighing the following raw materials in parts by weight: 3 parts of corn flour, 0.06 part of sodium hydroxide, 3 parts of hawthorn liquid, 6 parts of sweet potato, 3.5 parts of dried orange peel, 170 parts of deionized water, 4 parts of coptis powder, 3 parts of orange peel, 6 parts of glycerol, 2 parts of amino acid, 6 parts of sugar, 0.6 part of potassium chloride and 0.3 part of monopotassium phosphate;
B. taking pericarpium citri reticulatae, sugarcane and corn flour according to a ratio, adding deionized water, mixing, soaking for 1.3h, steaming at high temperature for 25 minutes, taking out, filtering filtrate through a double-layer metal filter screen, and preserving heat to obtain a mixed solution I;
C. taking hawthorn liquid and coptis chinensis powder according to a ratio, grinding orange peel into coarse powder, filtering the coarse powder to obtain fine powder, mixing the fine powder with the hawthorn liquid and the coptis chinensis powder, and then adding sodium hydroxide to adjust the pH value to obtain a mixed solution II;
D. and pouring sugar, glycerol and potassium chloride into the mixed solution I, uniformly mixing, pouring amino acid and potassium dihydrogen phosphate into the mixed solution II, uniformly mixing, mixing the mixed solution I and the mixed solution II together, and sterilizing at 103 ℃ and 106kPa for 11 minutes to obtain the hay compound culture medium.
Preferably: the pH value is 7.2, and after the pH value is adjusted, the mixed solution II is stirred for 35min at 83 ℃.
Preferably: after being ground into coarse powder, the citrus peel is filtered by a filter membrane with the diameter of 0.22 mu m to obtain fine powder.
Preferably: the sugar is one of sucrose, glucose, fructose, galactose, lactose and maltose.
Preferably: the amino acid is any one of alanine, valine, leucine, isoleucine, methionine, proline, tryptophan or serine.
Preferably: mixing the mixed solution I and the mixed solution II, sterilizing, sealing and packaging by using a plastic bag, stacking the sealed and packaged finished product in a storehouse at 22-28 ℃, performing anaerobic fermentation, and taking out.
Preferably: the anaerobic fermentation time is 32 h.
Preferably: the preparation method of the hawthorn juice comprises the following steps: removing core of fructus crataegi, decocting in water for 25min, taking out, and filtering twice with filter cloth to obtain fructus crataegi solution.
Preferably: the cooking temperature of the hawthorn is 85 ℃, and foam floating on the water surface is fished out during cooking.
Preferably: the heat preservation temperature of the mixed solution I is 32 ℃, and the mixed solution I is continuously stirred and activated for 1.3h while being kept warm.
In embodiment 2 of the invention, a production process of a hay compound culture medium comprises the following steps:
A. weighing the following raw materials in parts by weight: 4 parts of corn flour, 0.2 part of sodium hydroxide, 4 parts of hawthorn juice, 6.5 parts of sweet potato, 5 parts of dried orange peel, 175 parts of deionized water, 5 parts of coptis powder, 4 parts of orange peel, 7 parts of glycerol, 3 parts of amino acid, 7 parts of sugar, 1.1 parts of potassium chloride and 0.6 part of monopotassium phosphate;
B. taking pericarpium citri reticulatae, sugarcane and corn flour according to a ratio, adding deionized water, mixing, soaking for 1.6h, steaming at high temperature for 25 minutes, taking out, filtering filtrate through a double-layer metal filter screen, and preserving heat to obtain a mixed solution I;
C. taking hawthorn liquid and coptis chinensis powder according to a ratio, grinding orange peel into coarse powder, filtering the coarse powder to obtain fine powder, mixing the fine powder with the hawthorn liquid and the coptis chinensis powder, and then adding sodium hydroxide to adjust the pH value to obtain a mixed solution II;
D. and pouring sugar, glycerol and potassium chloride into the mixed solution I, uniformly mixing, pouring amino acid and potassium dihydrogen phosphate into the mixed solution II, uniformly mixing, mixing the mixed solution I and the mixed solution II together, and sterilizing at 106 ℃ and 111kPa for 11 minutes to obtain the hay compound culture medium.
Preferably: the pH value is 7.2, and after the pH value is adjusted, the mixed solution II is stirred for 35min at 85 ℃.
Preferably: after being ground into coarse powder, the citrus peel is filtered by a filter membrane with the diameter of 0.24 mu m to obtain fine powder.
Preferably: the sugar is one of sucrose, glucose, fructose, galactose, lactose and maltose.
Preferably: the amino acid is any one of alanine, valine, leucine, isoleucine, methionine, proline, tryptophan or serine.
Preferably: and mixing the mixed solution I and the mixed solution II together, sterilizing, sealing and packaging by using a plastic bag, stacking the sealed and packaged finished product in a storehouse at 25 ℃, performing anaerobic fermentation, and taking out.
Preferably: the anaerobic fermentation time is 35 h.
Preferably: the preparation method of the hawthorn juice comprises the following steps: removing core of fructus crataegi, decocting in water for 25min, taking out, and filtering twice with filter cloth to obtain fructus crataegi solution.
Preferably: the cooking temperature of the hawthorn is 88 ℃, and the foam on the water surface is fished out during cooking.
Preferably: the heat preservation temperature of the mixed solution I is 33 ℃, and the mixed solution I is continuously stirred and activated for 1.6h while being kept warm.
In embodiment 3 of the invention, a production process of a hay compound culture medium comprises the following steps:
A. weighing the following raw materials in parts by weight: corn flour 5 parts, sodium hydroxide 0.3 part, hawthorn liquid 7 parts, sweet potato 7 parts, dried orange peel 7 parts, deionized water 185 parts, coptis root powder 7 parts, orange peel 5 parts, glycerin 9 parts, amino acid 4 parts, sugar 9 parts, potassium chloride 1.3 parts and potassium dihydrogen phosphate 0.7 part;
B. taking pericarpium citri reticulatae, sugarcane and corn flour according to a ratio, adding deionized water, mixing, soaking for 1.7h, steaming at high temperature for 25 minutes, taking out, filtering filtrate through a double-layer metal filter screen, and preserving heat to obtain a mixed solution I;
C. taking hawthorn liquid and coptis chinensis powder according to a ratio, grinding orange peel into coarse powder, filtering the coarse powder to obtain fine powder, mixing the fine powder with the hawthorn liquid and the coptis chinensis powder, and then adding sodium hydroxide to adjust the pH value to obtain a mixed solution II;
D. and pouring sugar, glycerol and potassium chloride into the mixed solution I, uniformly mixing, pouring amino acid and potassium dihydrogen phosphate into the mixed solution II, uniformly mixing, mixing the mixed solution I and the mixed solution II together, and sterilizing at 107 ℃ under 114kPa for 11 minutes to obtain the hay compound culture medium.
Preferably: the pH value is 7.2, and after the pH value is adjusted, the mixed solution II is stirred for 35min at 87 ℃.
Preferably: after being ground into coarse powder, the citrus peel is filtered by a filter membrane with the diameter of 0.25 mu m to obtain fine powder.
Preferably: the sugar is one of sucrose, glucose, fructose, galactose, lactose and maltose.
Preferably: the amino acid is any one of alanine, valine, leucine, isoleucine, methionine, proline, tryptophan or serine.
Preferably: and mixing the mixed solution I and the mixed solution II together, sterilizing, sealing and packaging by using a plastic bag, stacking the sealed and packaged finished product in a storehouse at 27 ℃, performing anaerobic fermentation, and taking out.
Preferably: the anaerobic fermentation time is 38 h.
Preferably: the preparation method of the hawthorn juice comprises the following steps: removing core of fructus crataegi, decocting in water for 25min, taking out, and filtering twice with filter cloth to obtain fructus crataegi solution.
Preferably: the cooking temperature of the hawthorn is 98 ℃, and foam floating on the water surface is fished out during cooking.
Preferably: the temperature of the mixed solution I is 34 ℃, and the mixed solution I is continuously stirred and activated for 1.7 hours during temperature preservation.
Comparative example: sodium hydroxide, hawthorn juice, sweet potatoes, dried orange peels, coptis chinensis powder, orange peels, amino acids, sugar, potassium chloride and potassium dihydrogen phosphate are not added, and the rest components and steps are the same as those in the embodiment 1, the embodiment 2 and the embodiment 3;
the experimental method comprises the following steps:
4 transparent plastic tubes of 10cm in diameter and 30cm in height were prepared, washed, dried and sterilized before use, the culture media of examples 1, 2 and 3 and the control were injected, and the 4 transparent plastic tubes were placed in an incubator at 30-35 ℃ for culture, and the growth state of hyphae was observed every day, and the results are shown in the following table:
wherein: + + + indicates strong and dense growth of hyphae; + means weak and sparse hypha growth;
as can be seen from the above tables, the growth rates of the culture mediums in the transparent plastic tubes of examples 1, 2 and 3 are all > 3.0mm/d, the growth vigor is +++, the bacterial death rates are all < 2.0, the harmful bacterial growth rates are all < 1.0, the tube filling time is all > 10.0, the growth rate of the culture medium in the comparison example is slow, the growth vigor is weak, the bacterial death rate is high, the harmful bacterial growth rate is higher than 10.0, and the tube filling time is longer than 18, so that the culture medium prepared by the embodiment has the advantages of high growth rate, strong growth vigor, high bacteriostatic performance and stability, low content of generated pollutants and low pollution degree to the environment.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.
Claims (10)
1. A production process of a hay compound culture medium is characterized by comprising the following steps:
A. weighing the following raw materials in parts by weight: 2-6 parts of corn flour, 0.05-0.4 part of sodium hydroxide, 2-8 parts of hawthorn liquid, 5-8 parts of sweet potato, 3-8 parts of dried orange peel, 190 parts of deionized water, 3-8 parts of coptis powder, 2-6 parts of orange peel, 5-10 parts of glycerol, 1-5 parts of amino acid, 5-10 parts of sugar, 0.5-1.5 parts of potassium chloride and 0.2-0.8 part of potassium dihydrogen phosphate;
B. taking pericarpium citri reticulatae, sugarcane and corn flour according to a ratio, adding deionized water, mixing, soaking for 1.2-1.8h, steaming at high temperature for 25 minutes, taking out, filtering filtrate through a double-layer metal filter screen, and preserving heat to obtain a mixed solution I;
C. taking hawthorn liquid and coptis chinensis powder according to a ratio, grinding orange peel into coarse powder, filtering the coarse powder to obtain fine powder, mixing the fine powder with the hawthorn liquid and the coptis chinensis powder, and then adding sodium hydroxide to adjust the pH value to obtain a mixed solution II;
D. and pouring sugar, glycerol and potassium chloride into the mixed solution I for uniform mixing, pouring amino acid and potassium dihydrogen phosphate into the mixed solution II for uniform mixing, then mixing the mixed solution I and the mixed solution II together, and sterilizing for 11 minutes under the conditions of 102-108 ℃ and 105-115kPa to obtain the hay compound culture medium.
2. The production process of the hay compound culture medium according to claim 1, wherein the pH value is 7.2, and after the pH value is adjusted, the mixed solution II is stirred for 35min at 82-88 ℃.
3. The production process of the hay compound culture medium as claimed in claim 1, wherein the citrus peel is ground into coarse powder, the coarse powder is filtered through a 0.21-0.26 μm filter membrane, and fine powder is obtained after filtering.
4. The production process of the hay compound culture medium according to claim 1, wherein the sugar is any one of sucrose, glucose, fructose, galactose, lactose or maltose.
5. The production process of the hay compound culture medium according to claim 1, wherein the amino acid is any one of alanine, valine, leucine, isoleucine, methionine, proline, tryptophan or serine.
6. The production process of the hay compound culture medium according to claim 1, wherein the first mixed solution and the second mixed solution are mixed together and sterilized, then are hermetically packaged by a plastic bag, and the hermetically packaged finished product is stacked in a storehouse at 22-28 ℃, and is taken out after anaerobic fermentation.
7. The production process of the hay compound culture medium according to claim 6, wherein the anaerobic fermentation time is 30-40 h.
8. The production process of the hay compound culture medium according to claim 1, wherein the preparation method of the hawthorn juice comprises the following steps: removing core of fructus crataegi, decocting in water for 25min, taking out, and filtering twice with filter cloth to obtain fructus crataegi solution.
9. The production process of the hay compound culture medium as claimed in claim 8, wherein the cooking temperature of the hawthorn is 80-100 ℃, and froth on water surface is fished out during cooking.
10. The production process of the hay compound culture medium according to claim 1, characterized in that the temperature of the first mixed solution is 31-35 ℃, and the first mixed solution is continuously stirred and activated for 1.2-1.8 hours during temperature preservation.
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| CN202110504192.XA CN113201477A (en) | 2021-05-10 | 2021-05-10 | Production process of hay compound culture medium |
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| CN202110504192.XA CN113201477A (en) | 2021-05-10 | 2021-05-10 | Production process of hay compound culture medium |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103627649A (en) * | 2013-05-21 | 2014-03-12 | 郑州牧业工程高等专科学校 | Bacillus subtilis fermentation medium |
| CN105018397A (en) * | 2015-08-28 | 2015-11-04 | 济南舜昊生物科技有限公司 | Bacillus subtilis culture medium and preparation method thereof |
| CN107099479A (en) * | 2017-06-07 | 2017-08-29 | 成都众宜合生科技有限公司 | A kind of fermentation medium of bacillus subtilis |
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- 2021-05-10 CN CN202110504192.XA patent/CN113201477A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103627649A (en) * | 2013-05-21 | 2014-03-12 | 郑州牧业工程高等专科学校 | Bacillus subtilis fermentation medium |
| CN105018397A (en) * | 2015-08-28 | 2015-11-04 | 济南舜昊生物科技有限公司 | Bacillus subtilis culture medium and preparation method thereof |
| CN107099479A (en) * | 2017-06-07 | 2017-08-29 | 成都众宜合生科技有限公司 | A kind of fermentation medium of bacillus subtilis |
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