CN113201468A - Bacillus pumilus and application thereof in prevention and control of tomato nematode diseases - Google Patents
Bacillus pumilus and application thereof in prevention and control of tomato nematode diseases Download PDFInfo
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- CN113201468A CN113201468A CN202010541278.5A CN202010541278A CN113201468A CN 113201468 A CN113201468 A CN 113201468A CN 202010541278 A CN202010541278 A CN 202010541278A CN 113201468 A CN113201468 A CN 113201468A
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- bacillus pumilus
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- lksnx1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
Abstract
The invention discloses bacillus pumilus and application thereof in prevention and treatment of tomato root-knot nematode diseases. The strain provided by the invention is a strain of Bacillus pumilus (Bacillus pumilus) lksnx1, and the preservation number of the strain is CGMCC No. 19544. The bacillus pumilus lksnx1 has good biological control effect, a practical application method is simple and easy to operate, and the bacillus pumilus lksnx1 spore suspension has a remarkable inhibiting effect on the activity of the second-instar larvae of the root-knot nematodes; the inhibition effect of 2-fold diluent of spore suspension on hatching of nematode eggs is most obvious: after 4, 6, 8 and 10d, the egg hatchability was 93.5%, 92.1%, 72.7% and 82.7% lower than the control, respectively; the greenhouse bowl test shows that: the tomato is subjected to root irrigation treatment by using a 10-time diluent of a bacillus pumilus lksnx1 spore suspension, and the root knot number and the egg mass number on the root system are respectively 50.6 percent and 52.8 percent less than those of a control after 8 weeks. The invention provides a heavy strain material for the research and development of the tomato root-knot nematode disease biocontrol microbial inoculum and provides strain resources for the pollution-free production of tomatoes. The invention has great value for preventing and treating tomato root-knot nematode diseases. CGMCC No. 195442020.04.01.
Description
Technical Field
The invention relates to bacillus pumilus and application thereof in prevention and treatment of tomato nematode diseases.
Background
Nematodes are a plant parasitic disease of the world and seriously harm world agricultural production. Crops are threatened by various plant parasitic nematode groups, wherein the harm of root-knot nematodes is the most serious, and the root-knot nematodes are widely distributed and multi-parasitic and become the main pathogens threatening agricultural production worldwide and seriously affect the yield and quality of the crops. With the development of industry, the phenomena of continuous cropping and continuous cropping of vegetables are serious, which causes the harm of vegetable root-knot nematode disease (Meloidogyne incognita) to be more and more serious, wherein tomatoes, melons, cucumbers and eggplants are especially serious, the direct economic loss brought to vegetable growers reaches 20% -30%, and when the serious condition is up to 40% -50%, even the vegetable growers are out of production. Meanwhile, the plant disease inducing agent can enable fungi and bacteria to easily infect plants, and is one of important causes for inducing plant diseases.
At present, the prevention and treatment measures of nematode diseases comprise agricultural cultivation measures such as crop rotation and the like, breeding resistant varieties, chemical agent prevention and treatment and the like, and the measures have certain disadvantages. Agricultural cultivation measures such as crop rotation can prevent and control root-knot nematodes, but developing countries with too small per capita cultivated land area must rely on improving multiple cropping indexes to meet food supply. Although the breeding of resistant varieties can prevent and control the harm of nematodes, the application of the method is not wide at present, firstly, the method is limited by agronomic characters, and secondly, after a disease-resistant variety is continuously planted, the population of nematodes is changed, so that the number of a few nematode types which are not resistant by the variety is increased, and the resistance is gradually lost. The chemical agent has obvious effect of preventing and treating nematode diseases, but most chemical insecticides such as chloropicrin, potassium bromide, promecarpa and the like have high toxicity and overproof residues, and cause great pollution to the environment. In view of the disadvantages of the above methods, it is imperative to find a method for efficiently and safely controlling root-knot nematodes. Biological control controls nematode disease by antagonism, competition, induced resistance, and parasitism among microorganisms. The fungi for preventing and controlling nematode diseases reported at present comprise paecilomyces lilacinus, verticillium pachycete, gliocladium roseum, trichoderma harzianum, trichoderma viride, arthrobotrys and the like; the bacteria for preventing and controlling nematode diseases include pasteurella, bacillus, pseudomonas and the like.
Nematode diseases become a key subject of research and attention of agricultural experts and farmers, the traditional prevention and control method cannot fundamentally solve the problem, and biological prevention and control has great potential in controlling nematode diseases and improving plant yield.
Disclosure of Invention
The invention aims to provide a bacillus pumilus and application thereof in tomato nematode disease control.
The Bacillus pumilus (Bacillus pumilus) lksnx1 provided by the invention is preserved in China general microbiological culture Collection center (CGMCC) in 04/01 of 2020, the preservation number is CGMCC No.19544, and the preservation unit address is as follows: beijing in China. Bacillus pumilus (Bacillus pumilus) lksnx1 CGMCC No.19544 is abbreviated as Bacillus pumilus lksnx 1.
The invention also protects the application of the bacillus pumilus lksnx1 in the prevention and treatment of tomato nematode diseases. The tomato nematode disease is specifically caused by root-knot nematodes. The tomato can be specifically the tomato with the breed name of Xinmei No. 1.
The invention also provides a product for preventing and controlling the tomato nematode disease, and the active ingredient of the product is a spore suspension of the bacillus pumilus lksnx 1. The tomato nematode disease is specifically caused by root-knot nematodes. The tomato can be specifically the tomato with the breed name of Xinmei No. 1. The specific bacteria content of the spore suspension is 1 × 109CFU/ml. The preparation method of the spore suspension comprises the following specific steps: inoculating Bacillus pumilus lksnx1 to beef extract liquid culture medium, culturing at 32 deg.C under shaking at 180rpm for 48 hr, centrifuging at 5000rpm for 5min after spore formation is observed, collecting precipitate (spore), suspending the precipitate with sterile water to obtain spore concentration of 1 × 109CFU/ml spore suspension.
The application of the spore suspension of the bacillus pumilus lksnx1 in the prevention and treatment of tomato nematode diseases also belongs to the protection scope of the invention. The tomato nematode disease is specifically caused by root-knot nematodes. The tomato can be specifically the tomato with the breed name of Xinmei No. 1. The specific bacteria content of the spore suspension is 1 × 109CFU/ml. The preparation method of the spore suspension comprises the following specific steps: inoculating Bacillus pumilus lksnx1 into beef extract liquid culture medium, culturing at 32 deg.C under shaking at 180rpm for 48 hr, centrifuging at 5000rpm for 5min after spore formation is observed, collecting precipitate(spores) suspended and precipitated in sterile water to give a spore concentration of 1X 109CFU/ml spore suspension.
The application of the bacillus pumilus lksnx1 in the production of products for preventing and treating tomato nematode diseases also belongs to the protection scope of the invention. The tomato nematode disease is specifically caused by root-knot nematodes. The tomato can be specifically the tomato with the breed name of Xinmei No. 1.
The invention also provides a method for preventing and treating tomato nematode diseases, which comprises the step of root irrigation treatment by using the spore suspension of the bacillus pumilus lksnx1 after tomato seedlings are planted. The frequency of root irrigation treatment can be specifically carried out for 2-4 times according to the degree of disease attack of the tomato planting plot nematode. The tomato nematode disease is specifically caused by root-knot nematodes. The tomato can be specifically the tomato with the breed name of Xinmei No. 1. The specific bacteria content of the spore suspension is 1 × 109CFU/ml. The preparation method of the spore suspension comprises the following specific steps: inoculating Bacillus pumilus lksnx1 to beef extract liquid culture medium, culturing at 32 deg.C under shaking at 180rpm for 48 hr, centrifuging at 5000rpm for 5min after spore formation is observed, collecting precipitate (spore), suspending the precipitate with sterile water to obtain spore concentration of 1 × 109CFU/ml spore suspension.
The bacillus pumilus lksnx1 provided by the invention can effectively prevent and control tomato root-knot nematode diseases. The bacillus pumilus lksnx1 has a good field biocontrol effect, a simple practical application method and easy operation, and the bacillus pumilus lksnx1 spore suspension has a remarkable inhibiting effect on the activity of the second-instar larvae of the root-knot nematodes; the inhibition effect of 2-fold diluent of spore suspension on hatching of nematode eggs is most obvious: after 4, 6, 8 and 10d, the egg hatchability was 93.5%, 92.1%, 72.7% and 82.7% lower than the control, respectively; the greenhouse bowl test shows that: the tomato is subjected to root irrigation treatment by using a 10-time diluent of a bacillus pumilus lksnx1 spore suspension, and the root knot number and the egg mass number on the root system are respectively 50.6 percent and 52.8 percent less than those of a control after 8 weeks. The invention provides a heavy strain material for the research and development of the tomato root-knot nematode disease biocontrol microbial inoculum and provides strain resources for the pollution-free production of tomatoes. The invention has great value for preventing and treating tomato root-knot nematode diseases.
Claims (10)
1. Bacillus pumilus (Bacillus pumilus) lksnx1, which is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC No. 19544.
2. The use of the bacillus pumilus lksnx1 of claim 1 for the control of tomato nematode disease.
3. Use according to claim 2, characterized in that: the tomato nematode disease is caused by root-knot nematodes.
4. A product for controlling tomato nematode disease, the active ingredient of which is a spore suspension of the Bacillus pumilus lksnx1 of claim 1.
5. The product of claim 4, wherein: the tomato nematode disease is caused by root-knot nematodes.
6. The use of a spore suspension of the bacillus pumilus lksnx1 according to claim 1 for the control of tomato nematode disease.
7. Use according to claim 6, characterized in that: the tomato nematode disease is caused by root-knot nematodes.
8. Use of the bacillus pumilus lksnx1 in the manufacture of a product for use in the control of tomato nematode disease according to claim 1.
9. A method for controlling nematode disease in tomatoes comprising the step of root drenching a seedling of tomato with a suspension of bacillus pumilus lksnx1 spores as defined in claim 1.
10. The use according to claim 8 or the method according to claim 9, characterized by: the tomato nematode disease is caused by root-knot nematodes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010541278.5A CN113201468A (en) | 2020-06-16 | 2020-06-16 | Bacillus pumilus and application thereof in prevention and control of tomato nematode diseases |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010541278.5A CN113201468A (en) | 2020-06-16 | 2020-06-16 | Bacillus pumilus and application thereof in prevention and control of tomato nematode diseases |
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| CN113201468A true CN113201468A (en) | 2021-08-03 |
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Citations (6)
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| CN102283253A (en) * | 2011-08-31 | 2011-12-21 | 红云红河烟草(集团)有限责任公司 | Bacillus pumilus and application thereof on killing eelworms |
| US20130195826A1 (en) * | 2010-08-10 | 2013-08-01 | Chr. Hansen A/S | Composition comprising bacillus |
| CN103461389A (en) * | 2013-09-13 | 2013-12-25 | 成都天星农业科技有限公司 | Compound microorganism bacteria agent capable of preventing root-knot nematode and preparation method thereof |
| CN104010511A (en) * | 2011-11-04 | 2014-08-27 | 拜尔作物科学有限合伙公司 | Biocontrol of nematodes |
| CN106754586A (en) * | 2017-03-28 | 2017-05-31 | 南京林业大学 | One plant of pine endosymbiotic bacterium bacillus pumilus LYMC 3 and its application |
| CN109734517A (en) * | 2019-03-11 | 2019-05-10 | 云南省农业科学院生物技术与种质资源研究所 | A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition root-knot nematode |
-
2020
- 2020-06-16 CN CN202010541278.5A patent/CN113201468A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130195826A1 (en) * | 2010-08-10 | 2013-08-01 | Chr. Hansen A/S | Composition comprising bacillus |
| CN102283253A (en) * | 2011-08-31 | 2011-12-21 | 红云红河烟草(集团)有限责任公司 | Bacillus pumilus and application thereof on killing eelworms |
| CN104010511A (en) * | 2011-11-04 | 2014-08-27 | 拜尔作物科学有限合伙公司 | Biocontrol of nematodes |
| CN103461389A (en) * | 2013-09-13 | 2013-12-25 | 成都天星农业科技有限公司 | Compound microorganism bacteria agent capable of preventing root-knot nematode and preparation method thereof |
| CN106754586A (en) * | 2017-03-28 | 2017-05-31 | 南京林业大学 | One plant of pine endosymbiotic bacterium bacillus pumilus LYMC 3 and its application |
| CN109734517A (en) * | 2019-03-11 | 2019-05-10 | 云南省农业科学院生物技术与种质资源研究所 | A kind of microbial-bacterial fertilizer combines the method for fermented liquid inhibition root-knot nematode |
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Application publication date: 20210803 |