CN106754586A - One plant of pine endosymbiotic bacterium bacillus pumilus LYMC 3 and its application - Google Patents
One plant of pine endosymbiotic bacterium bacillus pumilus LYMC 3 and its application Download PDFInfo
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- CN106754586A CN106754586A CN201710193772.5A CN201710193772A CN106754586A CN 106754586 A CN106754586 A CN 106754586A CN 201710193772 A CN201710193772 A CN 201710193772A CN 106754586 A CN106754586 A CN 106754586A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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Abstract
The invention discloses one plant of pine endosymbiotic bacterium, Classification And Nomenclature is bacillus pumilus（Bacillus pumilus）LYMC 3, preservation and China typical culture collection center, deposit number is：CCTCC NO：M 2016775, preservation date：On December 21st, 2016, preservation address is Wuhan, China Wuhan University.Raw bacillus pumilus LYMC 3 in pine tree of the invention, be pine endosymbiotic bacterium, its aseptic ferment filtrate have it is very strong kill line effect, 2 times of the stoste of aseptic ferment filtrate and dilution, 4 times, after 10 times of liquid treatment pine wood nematode 24h, its death rate reaches 100%, and the polypide of death is all cleared up.The worm's ovum treated through aseptic ferment filtrate is not hatched, and the chorion of worm's ovum receives destruction, it is seen that the bacillus pumilus LYMC 3 says with good application prospect in terms of line is killed, with fine Biocontrol Effect.
Description
Technical field
The invention belongs to microbial technology field, and in particular to one plant of pine endosymbiotic bacterium bacillus pumilus LYMC-3 and
Its application.
Background technology
Pine tree wilt disease is colonized in Pinus seeds body by pine wood nematode (Bursaphelenchus xylophilus)
Take food nutrition and cause the rapid dead a kind of especially big destructive disease of trees, belong to international important quarantine object (Yang Baojun etc.,
2003), it is classified as first of forest disease and pest, is referred to as " smokeless forest fire ".The nematode be mainly distributed on the U.S., Canada,
Multiple countries such as Mexico, Japan, South Korea, Greece, China and Portugal.China from nineteen eighty-two Nanjing find since,
Be diffused into Jiangsu, Zhejiang, Anhui, Fujian, Jiangxi, Shandong, Henan, Hubei, Hunan, Guangdong, Guangxi, Chongqing, Sichuan, Guizhou,
The ground such as Shaanxi, Hong Kong and Taiwan, cause the massive losses on economy of forestry, forest ecology and the serious of natural landscape to break to China
It is bad, and pine forest to vast normal region constitutes a serious threat.In China, the primary vehicle of pine wood nematode is Monochamus alternatus
(Monochamus alternatus).The upper preventing and treating to the disease of production at present mainly by strengthening quarantine and clear up woodland to die of illness tree,
It is aided with the chemical prevention and biological control to Monochamus alternatus, and is directed to the preventing and treating of cause of disease pine wood nematode, especially pine wood nematode
Biological control research it is less.
Biological and ecological methods to prevent plant disease, pests, and erosion research currently for pine wood nematode is concentrated mainly on and kills a few class factors such as line plant, fungi and actinomyces
On, and the report that relevant bacterium is used as pine wood nematode bio-control factors is less, biological and ecological methods to prevent plant disease, pests, and erosion of the endophytic bacterium to pine wood nematode is studied
It is even more few.It is always study hotspot both domestic and external using endogenetic bacteria controlling plant diseases.Most plant diseases in the past
Biocontrol microorganisms separation screening from soil or plant rhizosphere soil microorganism is obtained.But because these edaphons are easily by outer
The influence of boundary's condition, and be difficult to enclosed with soil, plant rhizosphere or leaf practise occupy the competition of microorganism in long-term dominant, thus pole
Earth effect its practical value (Li Changsong, 1992).And endophytic bacterium has many good qualities as bio-control factors：They divide
It is distributed in the different tissues of plant, there is the nutriment of abundance, while protected by plant tissue, not by outside adverse circumstances
Such as strong sunlight, the influence of ultraviolet wind and rain, the ecological environment (tending to have gold etc., 2007) with stabilization.Therefore, endogenetic bacteria
It is more easy to play Biocontrol Effect relative to other bacteriums.The research for preventing and treating plant epiphyte and bacteriosis using endogenetic bacteria is a lot,
And in terms of nematodiasis is prevented and treated study it is little.
The content of the invention
Goal of the invention：It is short and small it is an object of the invention to provide life in one plant of pine tree for the deficiencies in the prior art
Bacillus, meets use demand.It is a further object of the present invention to provide the application of raw bacillus pumilus in one plant of pine tree.
Technical scheme：In order to realize foregoing invention purpose, the technical solution adopted by the present invention is：
One plant of pine endosymbiotic bacterium, Classification And Nomenclature is bacillus pumilus (Bacillus pumilus) LYMC-3, has been protected
Hide and China typical culture collection center, deposit number is：CCTCC NO：M 2016775, preservation date：In December, 2016
21, preservation address was Wuhan, China Wuhan University.
Applications of the described bacillus pumilus LYMC-3 in pine wood nematode is killed.
Applications of the described bacillus pumilus LYMC-3 in pine wood nematode ovum is killed.
A kind of method of the aseptic ferment filtrate for preparing described bacillus pumilus LYMC-3：Beef extract egg will be stored in
Bacillus pumilus LYMC-3 on white peptone solid medium accesses the triangular flask of the 50mL of fluid nutrient medium containing beef extract-peptone
In, in shaking training with 200r/min rotating speeds under 28 DEG C and non-illuminated conditions；The inoculum after training 4d will be shaken is with filter sizes
After 0.22 μm of biofilter filters thalline, the filtrate of gained is aseptic ferment filtrate.
The aseptic ferment filtrate that the method for the aseptic ferment filtrate of described preparation bacillus pumilus LYMC-3 is obtained.
Application of the described aseptic ferment filtrate in pine wood nematode is killed.
Application of the described aseptic ferment filtrate in pine wood nematode ovum is killed.
The present inventor from the Nanjing Zhongshan Tomb, Shanghai Chenshan Botanical Garden, Luoyang Sui Tang botanical garden black pine, masson pine, wetland
Pine and torch pine stem isolate 137 plants of endophytic bacterium, and 137 plants of bacteriums kill with the measure of line activity.Therefrom sieve
Selecting one plant of aseptic ferment filtrate has the bacterium LYMC-3 of strong toxic action to pine wood nematode and its worm's ovum.
The identification of LYMC-3：Bacterial strain LYMC-3 cultivates 48h on NA solid mediums, and bacterium colony is white wax-like, and edge is not
Neatly, dry, there is gauffer, bacterium colony is of medium size.Cell is in shaft-like, Gram-positive, and size is (0.8~0.9) μ m
(1.5~3.0) μm, gemma oval, middle life, peritrichous has motility.Gram-positive, oxydase reaction is negative,
Catalase reacting positive, gelatin liquefaction positive, Starch Hydrolysis are positive, and methyl red test is negative, V-P reacting positives, and nitrate is also
Former reaction negative, indole test is negative.After 16~24h of Biolog identification cultures, LYMC-3 is measured for Bacillus
Pumilus, SIM value are 0.624.16S rDNA sequence analyses, the sequence length of sequencing obtained strains LYMC-3 is 1400bp
(shown in SEQ ID NO.1).Sequence in the 16SrDNA sequences of LYMC-3 and GenBank is carried out into BLAST to compare, with LYMC-
3 bacterial strain similitude highests are Bacillus pumilus, and homology reaches 99%.The homologous sequence and measure for obtaining will be compared
Sequence carries out the homology analysis of multiple sequence with ClustalX softwares, then using MEGA6.05 software building phyletic evolutions
Tree.By the systematic evolution tree that builds as can be seen that LYMC-3 and Bacillus pumilus belong to a hereditary branch together.It is final true
Bacterial strain LYMC-3 is determined for bacillus pumilus (Bacillus pumilus), is named as bacillus pumilus (Bacillus
pumilus)LYMC-3。
Beneficial effect：Compared with prior art, raw bacillus pumilus LYMC-3 in pine tree of the invention, is raw in pine tree
There is bacterium, its aseptic ferment filtrate the very strong line that kills to act on, stoste and dilution 2 times, 4 times, 10 times of liquid of aseptic ferment filtrate
After treatment pine wood nematode 24h, its death rate reaches 100%, and the polypide of death is all cleared up.It is treated through aseptic ferment filtrate
Worm's ovum do not hatch, and the chorion of worm's ovum receives destruction, it is seen that the bacillus pumilus LYMC-3 says tool in terms of line is killed
There is good application prospect, with fine Biocontrol Effect.
Brief description of the drawings
Fig. 1 is toxic action (polypide is cleared up) result figure of the aseptic ferment filtrates of LYMC-3 to pine wood nematode；
Fig. 2 is influence (chorion destruction) result figure of the aseptic ferment filtrates of LYMC-3 to worm's ovum.
Specific embodiment
With reference to specific embodiment, the present invention is described further.
The preparation of the aseptic ferment filtrates of bacillus pumilus LYMC-3 of embodiment 1
Beef extract-peptone will be stored in (per 1000mL distilled water 10g containing peptone, beef extract 3g, sodium chloride 5g, pH=
7.2~bacillus pumilus LYMC-3 7.4) on solid medium accesses the three of the 50mL of fluid nutrient medium containing beef extract-peptone
In the bottle of angle, in shaking training with 200r/min rotating speeds under 28 DEG C and non-illuminated conditions.The inoculum after training 4d will be shaken and use filter membrane hole
Footpath is that after 0.22 μm of biofilter filters thalline, the filtrate of gained is the aseptic ferment filtrate in experiment.
The aseptic ferment filtrates of bacillus pumilus LYMC-3 of embodiment 2 kill the measure of line activity
The preparation of culture and nematode suspension for examination nematode：It is harmful to by Jiangsu Province of Nanjing Forestry University for examination pine wood nematode
Biotic intrusion prevents to be provided with control stress point laboratory, is located away from the black pine of Jiangsu Province Lianyungang Area morbidity.Pine wood nematode
Cultivated using Botrytis cinerea (Botrytis cinerca), nematode is seeded on the PDA plate for covering with Botrytis cinerea, be positioned over
25 DEG C of incubator cultures to Botrytis cinerea mycelia disappears, and nematode covers with flat board.Nematode is separated and collected using modified Baermann funnel method, so
Centrifugation sterilization concentration afterwards is configured to the nematode suspension of about 20/μ L.
Kill line determination of activity：The aseptic ferment filtrates of bacillus pumilus LYMC-3 (prepared by embodiment 1) that will be prepared are respectively
2 times, 4 times and 10 times of dilution, takes in the aseptic ferment filtrate injection 1.5mL centrifuge tubes of 1mL difference extension rates, reinjects 50 μ L
About 1000 nematodes of nematode suspension, mixing.Mixed liquor about 50 nematodes of 50 μ L are drawn after 12h and 24h is processed stereoscopic
Basis of microscopic observation nematode death condition and polypide clear up situation.Often process in triplicate, trained with the NA liquid for not connecing bacterium
It is control to support base and clear water.Nematode death standard is stiff polypide, is stirred with dissecting needle or still inertia during contact stimulus.
Fragmentation effect of the aseptic ferment filtrates of bacillus pumilus LYMC-3 of table 1 to pine wood nematode
As a result (table 1) shows：Pine wood nematode is through the aseptic ferment filtrate stostes of bacillus pumilus LYMC-3 and 2 times of dilution, 4
Again, after 10 times of liquid treatment 24h, its death rate reaches 100%, and the polypide of death is all cleared up (Fig. 1).Illustrate that LYMC-3 is aseptic
Ferment filtrate has and extremely strong kills line activity.
Influence of the aseptic ferment filtrates of bacillus pumilus LYMC-3 of embodiment 3 to pine wood nematode ovum
Pine wood nematode is collected using modified Baermann funnel method, a number of female, male imago of picking is in small culture dish plus suitable
Amount clear water, is positioned over culture in 25 DEG C of constant incubators.When nematode output a large amount of ovum, and culture dish is rinsed repeatedly with clear water,
Because worm's ovum can be attached on culture dish bottom surface, culture dish bottom can be still retained in when nematode is rinsed.Under the microscope
Observation, it is ensured that after existing without nematode in culture dish, take the aseptic ferment filtrates of 2mL (prepared by embodiment 1) stoste and be injected into small culture
In ware, moisturizing culture in 25 DEG C of constant incubators is positioned over.Control is processed as with clear water, each 3 repetition for the treatment of.Respectively at place
Reason 24h, 48h, 72h, 96h, examine under a microscope the hatching situation of worm's ovum, count incubation rate.
Influence of the aseptic ferment filtrates of bacillus pumilus LYMC-3 of table 2 to pine wood nematode egg hatching rate
As a result (table 2) shows：The worm's ovum treated through the aseptic ferment filtrates of bacillus pumilus LYMC-3 is not hatched,
It was additionally observed that the chorion of worm's ovum receives destruction (Fig. 2) under high-power microscope.And compare worm's ovum the incubating after 96h of clear water treatment
Rate is 87%.Illustrate that aseptic ferment filtrate can suppress the normal incubation process of worm's ovum.
SEQUENCE LISTING
<110>Nanjing Forestry University
<120>One plant of pine endosymbiotic bacterium bacillus pumilus LYMC-3 and its application
<130> 100
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1400
<212> DNA
<213> Bacillus pumilus
<400> 1
tgctatacat gcaagtcgag cggacagaag ggagcttgct cccggatgtt agcggcggac 60
gggtgagtaa cacgtgggta acctgcctgt aagactggga taactccggg aaaccggagc 120
taataccgga tagttccttg aaccgcatgg ttcaaggatg aaagacggtt tcggctgtca 180
cttacagatg gacccgcggc gcattagcta gttggtgggg taatggctca ccaaggcgac 240
gatgcgtagc cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact 300
cctacgggag gcagcagtag ggaatcttcc gcaatggacg aaagtctgac ggagcaacgc 360
cgcgtgagtg atgaaggttt tcggatcgta aagctctgtt gttagggaag aacaagtgcg 420
agagtaactg ctcgcacctt gacggtacct aaccagaaag ccacggctaa ctacgtgcca 480
gcagccgcgg taatacgtag gtggcaagcg ttgtccggaa ttattgggcg taaagggctc 540
gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga 600
aactgggaaa cttgagtgca gaagaggaga gtggaattcc acgtgtagcg gtgaaatgcg 660
tagagatgtg gaggaacacc agtggcgaag gcgactctct ggtctgtaac tgacgctgag 720
gagcgaaagc gtggggagcg aacaggatta gataccctgg tagtccacgc cgtaaacgat 780
gagtgctaag tgttaggggg tttccgcccc ttagtgctgc agctaacgca ttaagcactc 840
cgcctgggga gtacggtcgc aagactgaaa ctcaaaggaa ttgacggggg cccgcacaag 900
cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc 960
tctgacaacc ctagagatag ggctttccct tcggggacag agtgacaggt ggtgcatggt 1020
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat 1080
cttagttgcc agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa 1140
ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat 1200
ggacagaaca aagggctgca agaccgcaag gtttagccaa tcccataaat ctgttctcag 1260
ttcggatcgc agtctgcaac tcgactgcgt gaagctggaa tcgctagtaa tcgcggatca 1320
gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt 1380
ttgcaacacc cgaagtcggt 1400
Claims (7)
1. one plant of pine endosymbiotic bacterium, Classification And Nomenclature is bacillus pumilus（Bacillus pumilus）LYMC-3, preservation
With China typical culture collection center, deposit number is：CCTCC NO：M 2016775, preservation date：December 21 in 2016
Day, preservation address is Wuhan, China Wuhan University.
2. applications of the bacillus pumilus LYMC-3 described in claim 1 in pine wood nematode is killed.
3. applications of the bacillus pumilus LYMC-3 described in claim 1 in pine wood nematode ovum is killed.
4. a kind of method of the aseptic ferment filtrate of the bacillus pumilus LYMC-3 prepared described in claim 1, its feature exists
In：The bacillus pumilus LYMC-3 that will be stored on beef extract-peptone solid medium accesses liquid containing beef extract-peptone
In the triangular flask of culture medium 50mL, in shaking training with 200r/min rotating speeds under 28 DEG C and non-illuminated conditions；The bacterium after training 4d will be shaken
Nutrient solution filter sizes are that after 0.22 μm of biofilter filters thalline, the filtrate of gained is aseptic ferment filtrate.
5. the aseptic hair that the method for the aseptic ferment filtrate of the preparation bacillus pumilus LYMC-3 described in claim 4 is obtained
Ferment filtrate.
6. application of the aseptic ferment filtrate described in claim 5 in pine wood nematode is killed.
7. application of the aseptic ferment filtrate described in claim 5 in pine wood nematode ovum is killed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111742903A (en) * | 2020-06-22 | 2020-10-09 | 黄丽 | Method for controlling quantity of sanitary pests in Xinjiang livestock breeding place |
| CN113201468A (en) * | 2020-06-16 | 2021-08-03 | 中农绿康（北京）生物技术有限公司 | Bacillus pumilus and application thereof in prevention and control of tomato nematode diseases |
| CN113678843A (en) * | 2021-08-06 | 2021-11-23 | 南京林业大学 | New application of bacillus pumilus LYMC-3 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011069A (en) * | 2007-02-13 | 2007-08-08 | 湖南省植物保护研究所 | Microbial insecticide for controlling wireworm and application thereof |
| CN101899408A (en) * | 2010-06-29 | 2010-12-01 | 南京林业大学 | Bacillus pumilus and application thereof for killing bursaphelenchus xylophilus with poison |
| CN101914474A (en) * | 2010-07-28 | 2010-12-15 | 南京林业大学 | Ectomycorrhizal helper bacteria bacillus pumilus and application thereof |
| CN102630534A (en) * | 2012-04-24 | 2012-08-15 | 南京林业大学 | Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes |
| CN103205385A (en) * | 2013-04-22 | 2013-07-17 | 南京林业大学 | Bacterial strain Smal-007 for controlling pine wilt disease and application of bacterial strain |
-
2017
- 2017-03-28 CN CN201710193772.5A patent/CN106754586B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101011069A (en) * | 2007-02-13 | 2007-08-08 | 湖南省植物保护研究所 | Microbial insecticide for controlling wireworm and application thereof |
| CN101899408A (en) * | 2010-06-29 | 2010-12-01 | 南京林业大学 | Bacillus pumilus and application thereof for killing bursaphelenchus xylophilus with poison |
| CN101914474A (en) * | 2010-07-28 | 2010-12-15 | 南京林业大学 | Ectomycorrhizal helper bacteria bacillus pumilus and application thereof |
| CN102630534A (en) * | 2012-04-24 | 2012-08-15 | 南京林业大学 | Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes |
| CN103205385A (en) * | 2013-04-22 | 2013-07-17 | 南京林业大学 | Bacterial strain Smal-007 for controlling pine wilt disease and application of bacterial strain |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113201468A (en) * | 2020-06-16 | 2021-08-03 | 中农绿康（北京）生物技术有限公司 | Bacillus pumilus and application thereof in prevention and control of tomato nematode diseases |
| CN111742903A (en) * | 2020-06-22 | 2020-10-09 | 黄丽 | Method for controlling quantity of sanitary pests in Xinjiang livestock breeding place |
| CN113678843A (en) * | 2021-08-06 | 2021-11-23 | 南京林业大学 | New application of bacillus pumilus LYMC-3 |
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| CN106754586B (en) | 2019-10-18 |
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Application publication date: 20170531 Assignee: MAANSHAN Panshi Biotechnology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: X2019320000150 Denomination of invention: One plant of pine endosymbiotic bacterium bacillus pumilus LYMC-3 and its application License type: Common License Record date: 20191022 Application publication date: 20170531 Assignee: Nanjing Dawson Biotechnology Co., Ltd Assignor: Nanjing Forestry University Contract record no.: X2019320000151 Denomination of invention: One plant of pine endosymbiotic bacterium bacillus pumilus LYMC-3 and its application License type: Common License Record date: 20191022 Application publication date: 20170531 Assignee: Nanjing Forest Protection Bird Biotechnology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: X2019320000149 Denomination of invention: One plant of pine endosymbiotic bacterium bacillus pumilus LYMC-3 and its application License type: Common License Record date: 20191022 |