CN110172422A - One plant of molten albumen bacillus and its application in prevention and treatment root-knot nematode - Google Patents
One plant of molten albumen bacillus and its application in prevention and treatment root-knot nematode Download PDFInfo
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Abstract
This disclosure relates to one plant of molten albumen bacillus and its application in prevention and treatment root-knot nematode.The bacterial strain is molten albumen bacillus (Bacillus proteolyticus) Bp-196, is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 21st, 2018, culture presevation number is CGMCC NO.16525.The bacterial strain is the bacterium being separated to from the cucumber rhizosphere soil that root knot nematode disease occurs, and there is good control efficiency to help to realize safety, the No-harmful apple orchard of vegetables conducive to the harm of early stage prevention and control root-knot nematode root-knot nematode.
Description
Technical field
This disclosure relates to which microbe application field, is specifically related to one plant of molten albumen bacillus and its in prevention and treatment root knot line
Application in worm.
Background technique
Plant nematode is in the presence of many countries and regions, type are varied in the world.Wherein, root-knot nematode is most heavy
One of plant nematode wanted, with increasing sharply for China's facilities vegetable cultivated area and constantly mentioning for vegetables multiple crop index
The occurring area of height, root-knot nematode is gradually expanded, and can make the vegetables underproduction 20%~30% or even 60%~70%, serious to restrict
The development of facilities vegetable industry.The host of root-knot nematode (M.incognita) is more than 3000 various plants, can almost be infected
The root of all cultivated plants.
Current produce above mainly uses chemical nematicides prevention and treatment root-knot nematode, but the generally existing poison of chemical nematicides
Property is larger, pollutes environment, is also easy to produce the problems such as drug resistance, therefore people gradually reduce the use of chemical agent in practice
Amount.Due to biocontrol bacterial strain have the characteristics that it is free from environmental pollution, do not develop drug resistance, to safety of human and livestock, filter out efficient
The biocontrol bacterial strain for preventing and treating root-knot nematode becomes hot spot studied both at home and abroad at present.
Summary of the invention
In order to overcome problems of the prior art, the disclosure provides one plant of molten albumen bacillus Bp-196, the bacterium
Strain is the bacterium being separated to from the cucumber rhizosphere soil that root knot nematode disease occurs, and root-knot nematode is imitated with good prevention and treatment
Fruit helps to realize safety, the No-harmful apple orchard of vegetables conducive to the harm of early stage prevention and control root-knot nematode.
The bacterial strain that the disclosure provides is molten albumen bacillus (Bacillus proteolyticus) Bp-196, is by river
North saves separation, purifying in the cucumber rhizosphere soil that root knot nematode disease occurs in Langfang City plantation cucumber field and obtains, in
It is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 21st, 2018, address: Beijing's southern exposure
The institute of area North Star West Road 1, Institute of Microorganism, Academia Sinica, culture presevation number is CGMCC NO.16525.It has with
Lower biological characteristics: on LB culture medium flat plate, after cultivating 3 days at 30 DEG C, the bacterium colony of the bacterial strain is big, and rough surface is flat,
Irregularly, inner ring color is darker, and all bacterial strains are Gram-positive.It has been observed that institute is germy thin under scanning electron microscope
Born of the same parents are in rodlike and have flagellum abundant, are respectively formed elliptic spore in swelling sporangium.
In one example, the molten albumen bacillus Bp-196 bacterial strain that the disclosure provides is gram positive bacterial strain.
In one example, the fermentation process for the molten albumen bacillus Bp-196 bacterial strain that the disclosure provides, wherein this method packet
It includes: by molten albumen bacillus Bp-196 strain inoculated into fermentation medium, at 30 DEG C, with the revolving speed culture 3 of 220rpm
It.
In one example, fermentation medium includes: 5g/L yeast powder, 10g/L peptone, and 10g/L sodium chloride utilizes distilled water
It is settled to 1L.
In one example, the molten albumen bacillus Bp-196 bacterial strain that the disclosure provides is applied to manufacture prevention and treatment root knot nematode disease
Medication in.
The molten albumen bacillus Bp-196 bacterial strain that the disclosure provides is from the cucumber rhizosphere soil that root knot nematode disease occurs
In the bacterium that is separated to.On the one hand, the bacterial strain is harmless to crop safety, helps to realize safety, the No-harmful apple orchard of vegetables；Separately
On the one hand, which has good control efficiency for root-knot nematode.It is shown experimentally that, the bacterial strain fermentation liquor is to root knot line
The lethality of worm second instar larvae reaches 100%, in controlling experiment, reaches 86.5% to the control efficiency of root-knot nematode, prevention and treatment
Effect is efficiently and stable.
Detailed description of the invention
Fig. 1 is the bacterium colony figure of molten albumen bacillus Bp-196 bacterial strain in some embodiments of the disclosure；
Fig. 2 is the ne ar figure of molten albumen bacillus Bp-196 bacterial strain shown in FIG. 1.
Specific embodiment
The principle and spirit of the disclosure are described below with reference to several illustrative embodiments.It should be appreciated that providing this
A little embodiments are used for the purpose of making those skilled in the art can better understand that realizing the disclosure in turn, and be not with any
Mode limits the scope of the present disclosure.
Embodiment 1: the separation of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain, purifying with
Identification
1. the isolation and purification of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain
Molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain of the disclosure is by Hebei province corridor
Mill city plants cucumber field and occurs to use soil dilution method isolated in the cucumber rhizosphere soil of root knot nematode disease, point
From method are as follows:
Pedotheque is uniformly acquired from the cucumber rhizosphere in Hebei province's Langfang City green cucumber field, amounts to six parts, every part
10g is respectively taken, and 6 parts of soil of acquisition are uniformly mixed.Mixed 60g pedotheque is added in 1000mL distilled water and is stirred
Uniformly, after standing 1min, its supernatant liquor is taken, and the supernatant liquor is placed in heating water bath 30min in 80 DEG C of water-bath, water
Supernatant liquor can be sufficiently stirred during bath, so that it is heated evenly.It is cold to supernatant liquor after heating water bath
But it to room temperature, is shaken up, and takes 1mL clear liquid, the clear liquid is diluted 100 times using aqua sterilisa.Then the clear liquid after taking dilution
0.25ml is coated on LB plate, is smeared uniformly, and 2~4d of culture in 30 DEG C of constant incubators is placed in, and the life of bacterium colony is observed in timing
Long situation.
It should be noted that the production method of LB plate is: taking 5g/L yeast powder, 10g/L peptone, 10g/L sodium chloride
It is placed in culture dish with 0.13-0.15g/L agar powder, is settled to 1L using distilled water, obtains LB plate base fluid；Then LB is put down
Plate base fluid autoclave sterilization 20 minutes at 121 DEG C, and plate is made in culture dish middle berth, to obtain LB plate.
After tissue block grows bacterium colony, single spore separation is carried out, picking single colonie purifies on LB culture medium.
2. the identification of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain
(1) Microbiological Characteristics:
As shown in Figs. 1-2, the bacterial strain of acquisition is on LB culture medium flat plate, after being cultivated 3 days at 30 DEG C, the bacterium colony of the bacterial strain
Greatly, rough surface, flat, irregularly, inner ring color is darker, and all bacterial strains are Gram-positive.It is observed under scanning electron microscope
It was found that the germy cell of institute is in rodlike and has flagellum abundant, elliptic spore is respectively formed in swelling sporangium.According to
" primary Jie Shi Bacteria Identification handbook " carries out Morphological Identification to the bacterial strain, determines that the bacterial strain is molten albumen bacillus
(Bacillus proteolyticus)。
(2) molecular biological characteristic:
It with the toothpick picking single colonie of sterilizing, is put into the EP pipe for filling the 300 thin lysate SDS of μ L bacterium, 65 DEG C of cracking 2 are small
When, DNA of bacteria then, which is extracted, with bacterial genomes DNA extraction kit is carried out using DNA as template with primer 2 7F and 1492R
16s rRNA sequence amplification.Pcr amplification reaction system is 50 μ L, contains 25 μ L ExTaq enzymes, 1 μ L forward primer, and 1 μ L reversely draws
Object, 3 μ L DNA profilings, 20 μ L of sterile water.The condition of amplification is: 94 DEG C of initial denaturation 5min, 94 DEG C of denaturation 30s, 45 DEG C are annealed
30s, 72 DEG C of extension 2.5min, 30 circulations；72 DEG C of extension 10min.Amplified production is separated through 1% agarose gel electrophoresis and is reflected
Fixed, PCR product directly carries out bidirectional sequencing.It should be noted that above-mentioned bacterial genomes DNA extraction kit is selected from Tiangeng
Biotechnology Co., Ltd.
16S rRNA sequence amplification is carried out with primer 1492R, 27F, PCR product is through 1% agarose gel electrophoresis.Through being sequenced
Analysis, a length of 1509bp of 16s rRNA sequence of bacterial strain amplification.The 16S rRNA sequence of the bacterial strain is subjected to BLAST analysis
Afterwards, the results show that the bacterial strain and molten albumen bacillus (Bacillus proteolyticus) similarity have reached 100%.
Identify that the bacterial strain is molten albumen bacillus (Bacillus proteolyticus).The 16s rDNA gene order of the bacterial strain is surveyed
Determine following (SEQ No.1): the AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGT of result
CGAGCGAATGGATTAAGAGCTTGCTCTTATGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCAT
AAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACATTTTGAACTGCATGGTTCGAAATTGAAAGGC
GGCTTCGGCTGTCACTTATGGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGA
TGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGT
AGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAAC
TCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTA
ACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGG
TGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAG
AAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTT
TCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGT
AAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGG
AGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA
AGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGA
GTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTT
GATCTTAGTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACG
TCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAGACCGCGA
GGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCT
AGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTT
TGTAACACCCGAAGTCGGTGGGGTAACCTTTTTGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAAGTC
GTAACAAGGT。
This bacterial strain was stored in China Committee for Culture Collection of Microorganisms's common micro-organisms on September 21st, 2018
The heart, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, culture presevation number is CGMCC
NO.16525。
Embodiment 2: the nematicidal effect of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain
1. prepared by the fermentation liquid of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain
By molten albumen bacillus Bp-196 strain inoculated into fermentation medium, at 30 DEG C, with the revolving speed of 220rpm
Culture 3 days, to obtain the fermentation liquid of molten albumen bacillus Bp-196 bacterial strain.Wherein, fermentation medium includes: 5g/L yeast
Powder, 10g/L peptone, 10g/L sodium chloride are settled to 1L using distilled water.
2. nematode is used in preparation test
Root-knot nematode picks up from Vegetable & Flower Inst., Chinese Academy of Agriculture Science greenhouse.By the root of the capsicum of root-knot nematode morbidity
System takes out, and is gently rinsed with water, carefully removes pieces of an egg from root system surface, be placed in 1% sodium hypochlorite and sterilize 3min, then use nothing
Bacterium water rinses 3 times, is placed in the culture dish containing a small amount of sterile water, cultivates in 25 DEG C of insulating boxs, and -48h collects hatching for 24 hours
Root-knot nematode second instar larvae is used for experimental study with sterile aqueous suspension.
3. test method
Take molten albumen bacillus Bp-196 bacterial strain fermentation liquid and its 10 times, 100 times of dilutions handle above-mentioned respectively
Tie lines worm second instar larvae measures molten albumen bacillus Bp-196 fermentation liquid to the kill effect of root-knot nematode.
12 24 sterile porocyte culture plates are taken, 12 tissue culture plates are divided into four groups, every group of test is repeated 3 times,
And fermentation liquid, 10 times of fermentation liquids of dilution, dilution 100 times of fermentation liquids and sterile are added into hole respectively in every group of tissue culture plate
Each 1mL of water, wherein zymotic fluid group will be added, 10 times of zymotic fluid groups of dilution are added and 100 times of zymotic fluid groups of dilution are added as examination
Group is tested, sterile water group will be added as a control group, 100 μ L nematode suspension are then added into four groups of tissue culture plates respectively
(about 100 nematodes), is placed on the death condition cultivated observe root-knot nematode for 24 hours at room temperature, and calculate corrected mortality, as
Nematicidal effect.
Corrected mortality (%)=(test group root-knot nematode average mortality-control group root-knot nematode average mortality)/
(1- control group root-knot nematode average mortality) * 100%
Table 1 is the examination of the fermentation liquid killing root-knot nematode second instar larvae of the molten albumen bacillus Bp-196 bacterial strain of various concentration
Test result.
The test of the fermentation liquid killing root-knot nematode second instar larvae of the molten albumen bacillus Bp-196 bacterial strain of 1 various concentration of table
As a result
| The death rate (%) | Zymotic fluid group | Dilute 10 times of zymotic fluid groups | Dilute 100 times of zymotic fluid groups | Sterile water group |
| Repeat test 1 | 100 | 94.5 | 10.2 | 1.2 |
| Repeat test 2 | 100 | 90.7 | 9.8 | 1.1 |
| Repeat test 3 | 100 | 92.2 | 9.6 | 1.1 |
| Average mortality | 100 | 92.5 | 9.9 | 1.1 |
| Corrected mortality | 100 | 92.5 | 9.9 | — |
It can be seen that the molten albumen bacillus Bp-196 fermentation liquid of different extension rates to nematode by above-mentioned test
Function and effect are different.When not being diluted to fermentation liquid, the insecticidal effect to root-knot nematode is 100%；When to fermentation liquid
When diluting 10 times, 92.5% is still reached to the insecticidal effect of root-knot nematode；And when diluting 100 times to fermentation liquid, it is right
The insecticidal effect of root-knot nematode is very low, and only 9.9%, the difference very little with control group illustrates molten albumen bacillus Bp-196
The fermentation liquid of bacterial strain has good prevention and treatment root-knot nematode effect in higher concentrations.
Embodiment 3: the nematicidal effect of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain
1. prepared by the fermentation liquid of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain
By molten albumen bacillus Bp-196 strain inoculated into fermentation medium, at 30 DEG C, with the revolving speed of 220rpm
Culture 3 days, to obtain the fermentation liquid of molten albumen bacillus Bp-196 bacterial strain.Wherein, fermentation medium includes: 5g/L yeast
Powder, 10g/L peptone, 10g/L sodium chloride are settled to 1L using distilled water.
2. nematode is used in preparation test
Root-knot nematode picks up from Vegetable & Flower Inst., Chinese Academy of Agriculture Science greenhouse.By the root of the capsicum of root-knot nematode morbidity
System takes out, and is gently rinsed with water, carefully removes pieces of an egg from root system surface, be placed in 1% sodium hypochlorite and sterilize 3min, then use nothing
Bacterium water rinses 3 times, is placed in the culture dish containing a small amount of sterile water, cultivates in 25 DEG C of insulating boxs, and -48h collects hatching for 24 hours
Root-knot nematode second instar larvae is used for experimental study with sterile aqueous suspension.
3. test method
It taking 180 plants of cucumber seedlings to be tested, 180 plants of cucumber seedlings is divided into two groups, every group of test is repeated 3 times, and
The fermentation liquid and each 15mL of sterile water of molten albumen bacillus Bp-196 bacterial strain are uniformly poured in every group of cucumber seedling soil respectively,
Wherein, using the cucumber seedling group for pouring molten albumen bacillus Bp-196 fermentation liquid as test group, the Huang of sterile water will be poured
Melon seedling group is as a control group.After 1 day, to the cucumber seedling of test group and control group be inoculated with root-knot nematode second instar larvae, every plant
Inoculation 500, at room temperature normal culture, and the root knot quantity after 5 weeks on the cucumber seedling of detection test group and control group,
Preventive effect of the fermentation liquid to root-knot nematode of molten albumen bacillus Bp-196 bacterial strain is calculated with this.
Control efficiency calculation formula:
Control efficiency (%)=(1- test group be averaged root knot quantity/control group be averaged root knot quantity) * 100%
Table 2 is that the fermentation liquid of molten albumen bacillus Bp-196 bacterial strain prevents the test result of root-knot nematode.
The test result of the fermentation liquid prevention root-knot nematode of the molten albumen bacillus Bp-196 bacterial strain of table 2
| Root knot number | Zymotic fluid group | Sterile water group |
| Repeat test 1 | 18.9 | 135.7 |
| Repeat test 2 | 17.1 | 124.5 |
| Repeat test 3 | 16.2 | 127.1 |
| Average root knot number | 17.4 | 129.1 |
It can be calculated according to control efficiency calculation formula, the fermentation liquid of molten albumen bacillus Bp-196 bacterial strain is to root
The control efficiency of tie lines worm has reached 86.5%.
It can be seen that the cucumber plant after the fermentation liquor treatment of molten albumen bacillus Bp-196 bacterial strain by above-mentioned test
On root knot quantity significantly reduce.In control group, 129.1/plant of root knot quantity average out to of cucumber, and through molten albumen bud
17.4/plant of root knot quantity average out in the cucumber of the fermentation liquor treatment of spore bacillus Bp-196 bacterial strain.It can be obtained by calculating
It arrives, the fermentation liquid of molten albumen bacillus Bp-196 bacterial strain has reached 86.5% to the control efficiency of root-knot nematode, illustrates molten egg
White bacillus Bp-196 bacterial strain can be with effectively preventing cucumber root-knot nematode.Have in agricultural production prevention and treatment root knot nematode disease
Important value.
For the purpose of example and description, the preceding description of disclosure implementation is had been presented for.Preceding description is not poor
The disclosure is restricted to exact form disclosed by also not the really wanting of act property, according to the above instruction there is likely to be various modifications and
Modification, or various changes and modifications may be obtained from the practice of the disclosure.Select and describe these embodiments and be in order to
Illustrate the principle and its practical application of the disclosure, so that those skilled in the art can be to be suitable for the special-purpose conceived
Come in a variety of embodiments with various modifications and using the disclosure.
Sequence table
<110>Vegetable & Flower Inst., Chinese Academy of Agriculture Science
<120>one plants of molten albumen bacillus and its application in prevention and treatment root-knot nematode
<130> ZLP1900183CN
<141> 2019-05-28
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1509
<212> DNA
<213>molten albumen bacillus (Bacillus proteolyticus)
<400> 1
agagtttgat cctggctcag gatgaacgct ggcggcgtgc ctaatacatg caagtcgagc 60
gaatggatta agagcttgct cttatgaagt tagcggcgga cgggtgagta acacgtgggt 120
aacctgccca taagactggg ataactccgg gaaaccgggg ctaataccgg ataacatttt 180
gaactgcatg gttcgaaatt gaaaggcggc ttcggctgtc acttatggat ggacccgcgt 240
cgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcgtag ccgacctgag 300
agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta 360
gggaatcttc cgcaatggac gaaagtctga cggagcaacg ccgcgtgagt gatgaaggct 420
ttcgggtcgt aaaactctgt tgttagggaa gaacaagtgc tagttgaata agctggcacc 480
ttgacggtac ctaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt 540
aggtggcaag cgttatccgg aattattggg cgtaaagcgc gcgcaggtgg tttcttaagt 600
ctgatgtgaa agcccacggc tcaaccgtgg agggtcattg gaaactggga gacttgagtg 660
cagaagagga aagtggaatt ccatgtgtag cggtgaaatg cgtagagata tggaggaaca 720
ccagtggcga aggcgacttt ctggtctgta actgacactg aggcgcgaaa gcgtggggag 780
caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta agtgttagag 840
ggtttccgcc ctttagtgct gaagttaacg cattaagcac tccgcctggg gagtacggcc 900
gcaaggctga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt 960
aattcgaagc aacgcgaaga accttaccag gtcttgacat cctctgaaaa ccctagagat 1020
agggcttctc cttcgggagc agagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg 1080
tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg atcttagttg ccatcattaa 1140
gttgggcact ctaaggtgac tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa 1200
tcatcatgcc ccttatgacc tgggctacac acgtgctaca atggacggta caaagagctg 1260
caagaccgcg aggtggagct aatctcataa aaccgttctc agttcggatt gtaggctgca 1320
actcgcctac atgaagctgg aatcgctagt aatcgcggat cagcatgccg cggtgaatac 1380
gttcccgggc cttgtacaca ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg 1440
gtggggtaac ctttttggag ccagccgcct aaggtgggac agatgattgg ggtgaagtcg 1500
taacaaggt 1509
Claims (5)
1. a kind of molten albumen bacillus (Bacillus proteolyticus) Bp-196 bacterial strain, the depositary institution of the bacterial strain
For China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC NO.16525.
2. molten albumen bacillus Bp-196 bacterial strain according to claim 1, wherein
The bacterial strain is gram positive bacterial strain.
3. the fermentation process of molten albumen bacillus Bp-196 bacterial strain described in claim 1, wherein
The described method includes:
By the molten albumen bacillus Bp-196 strain inoculated into fermentation medium, at 30 DEG C, with the revolving speed of 220rpm
Culture 3 days.
4. the fermentation process of molten albumen bacillus Bp-196 bacterial strain according to claim 3, wherein the fermented and cultured
Base includes: 5g/L yeast powder, 10g/L peptone, and 10g/L sodium chloride is settled to 1L using distilled water.
5. a kind of molten albumen bacillus Bp-196 bacterial strain described in any one of Claims 1-4 prevents and treats root knot line in manufacture
Purposes in the medication of parasitosis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910452123.1A CN110172422B (en) | 2019-05-28 | 2019-05-28 | Bacillus proteolicus and application thereof in preventing and controlling root-knot nematodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910452123.1A CN110172422B (en) | 2019-05-28 | 2019-05-28 | Bacillus proteolicus and application thereof in preventing and controlling root-knot nematodes |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116286550A (en) * | 2023-04-24 | 2023-06-23 | 中国农业大学 | Multifunctional Pr Sha Genliu bacterium, microbial agent containing same and application |
| CN117187103A (en) * | 2023-06-25 | 2023-12-08 | 大庆亿莱检验检测技术服务有限公司 | Petroleum degrading bacterium and application thereof |
| CN117487724A (en) * | 2023-12-29 | 2024-02-02 | 东北林业大学 | Bacillus proteolyticus CWJ-2-GJ and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479938A (en) * | 2016-12-09 | 2017-03-08 | 中国农业科学院蔬菜花卉研究所 | A kind of Brevibacillus brevis bacterial strain and its application |
| CN106754493A (en) * | 2016-12-09 | 2017-05-31 | 中国农业科学院蔬菜花卉研究所 | A kind of bacillus subtilis strain and its application |
| KR20180091594A (en) * | 2017-02-07 | 2018-08-16 | 안동대학교 산학협력단 | Bacillus subtilis strain YGB70 and microbial agent for prevention of ginseng root rot pathogens comprising the same |
| WO2019030529A1 (en) * | 2017-08-11 | 2019-02-14 | University Of Exeter | Biopesticides |
-
2019
- 2019-05-28 CN CN201910452123.1A patent/CN110172422B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106479938A (en) * | 2016-12-09 | 2017-03-08 | 中国农业科学院蔬菜花卉研究所 | A kind of Brevibacillus brevis bacterial strain and its application |
| CN106754493A (en) * | 2016-12-09 | 2017-05-31 | 中国农业科学院蔬菜花卉研究所 | A kind of bacillus subtilis strain and its application |
| KR20180091594A (en) * | 2017-02-07 | 2018-08-16 | 안동대학교 산학협력단 | Bacillus subtilis strain YGB70 and microbial agent for prevention of ginseng root rot pathogens comprising the same |
| WO2019030529A1 (en) * | 2017-08-11 | 2019-02-14 | University Of Exeter | Biopesticides |
Non-Patent Citations (5)
| Title |
|---|
| BHASKAR N等: "Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities.", 《BIORESOUR TECHNOL》 * |
| JING XIONG等: "Systemic nematicidal activity and biocontrol efficacy of bacillus firmus against the root-knot nematode Meloidogyne incognita.", 《WORLD J MICROBIOL BIOTECHNOL》 * |
| 张玉芹: "枯草芽孢杆菌Bs-18菌株分离与鉴定及对根结线虫的防治效果", 《北方园艺》 * |
| 成飞雪等: "生防细菌YC-10的杀线虫活性及其鉴定 ", 《植物病理学报》 * |
| 黄金玲等: "植物寄生线虫生防细菌的研究进展 ", 《广西农业生物科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116286550A (en) * | 2023-04-24 | 2023-06-23 | 中国农业大学 | Multifunctional Pr Sha Genliu bacterium, microbial agent containing same and application |
| CN116286550B (en) * | 2023-04-24 | 2023-08-04 | 中国农业大学 | Multifunctional Pr Sha Genliu bacterium, microbial agent containing same and application |
| CN117187103A (en) * | 2023-06-25 | 2023-12-08 | 大庆亿莱检验检测技术服务有限公司 | Petroleum degrading bacterium and application thereof |
| CN117187103B (en) * | 2023-06-25 | 2024-05-14 | 大庆亿莱检验检测技术服务有限公司 | Petroleum degrading bacterium and application thereof |
| CN117487724A (en) * | 2023-12-29 | 2024-02-02 | 东北林业大学 | Bacillus proteolyticus CWJ-2-GJ and application thereof |
| CN117487724B (en) * | 2023-12-29 | 2024-03-22 | 东北林业大学 | Bacillus proteolyticus CWJ-2-GJ and application thereof |
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| CN110172422B (en) | 2020-12-25 |
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