CN113201452A - Microbial spore's cultivation equipment - Google Patents
Microbial spore's cultivation equipment Download PDFInfo
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- CN113201452A CN113201452A CN202110517257.4A CN202110517257A CN113201452A CN 113201452 A CN113201452 A CN 113201452A CN 202110517257 A CN202110517257 A CN 202110517257A CN 113201452 A CN113201452 A CN 113201452A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/24—Recirculation of gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a microbial spore culture device, which belongs to the technical field of microbial culture and comprises an incubator and a controller, wherein the side wall of the incubator is fixedly connected with a supporting plate, the supporting plate is fixedly provided with the controller, the upper end and the lower end of an operating hole formed in the incubator are respectively provided with an arc-shaped guide rail, the arc-shaped guide rails are movably connected with an arc-shaped baffle through an arc-shaped groove, a shaking structure for shaking a culture dish is arranged in the incubator, the inner bottom of the incubator is connected with an oxygen supply heating structure, and the side wall of the incubator is fixedly connected with a temperature sensor and an oxygen sensor; the spores are in a relaxed state during propagation, mutual extrusion does not exist, the rapid growth of the spores is facilitated, the culture period is shortened, and the actual culture is facilitated.
Description
Technical Field
The invention relates to the technical field of microbial cultivation, in particular to a microbial spore cultivation device.
Background
Microbial spores are germ cells that can develop into new individuals directly or indirectly after leaving parents. It is the product of mitosis or meiosis; most are haploid and few are diploid. Spores are generally unicellular and may be multicellular propagules. Due to the difference of the properties, the occurrence process and the structure, the diversity of the spores is formed.
The spore culture of microbe needs spore culture medium, which is one kind of solid culture medium for strain to propagate spore and has the requirement of fast growth of thallus, high quality spore production and less variation of strain.
At present, the culture dish is filled with the fixed culture medium, the spores are moved to the top of the fixed culture medium for culture, the culture dish is in a fixed state, the spores are in a mutual extrusion state during spore proliferation, and the mutually extruded spores are not beneficial to fast growth, so that the culture period is prolonged.
Based on this, the present invention provides an apparatus for culturing spores of microorganisms to solve the above problems.
Disclosure of Invention
Solves the technical problem
In view of the above-mentioned disadvantages of the prior art, the present invention provides an apparatus for culturing microbial spores.
Technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
the utility model provides a cultivation equipment of microorganism spore, includes incubator and controller, the lateral wall fixedly connected with backup pad of incubator, backup pad fixed mounting has the controller, the lower extreme all is provided with the arc guide rail about the handle hole that the incubator was seted up, arc guide rail has cowl through the arc wall swing joint who sets up, be provided with the shake structure that is used for the culture dish shake in the incubator, the interior bottom of incubator is connected with the oxygen suppliment heating structure, the lateral wall fixedly connected with temperature-sensing ware and the oxygen inductor of incubator.
Furthermore, fixedly connected with glass window in cowl's the through-hole, cowl's inner wall fixedly connected with and incubator sealing contact's sealing washer, cowl's outer end left side is connected with the push pedal.
Furthermore, the shaking structure is also provided with a mounting structure for mounting a culture dish.
Furthermore, the shaking structure comprises a Z-shaped support frame, a driving motor, a first bevel gear, a second bevel gear, a rotating shaft, a connecting block, a rotating block, a transverse rod, an arc-shaped plate, a rotating rod and a straight groove, the Z-shaped support frame is fixedly arranged at the bottom of the incubator, the driving motor is fixedly arranged on the Z-shaped support frame, the output end of the driving motor is fixedly connected with the first bevel gear, the first bevel gear is connected with the second bevel gear in a meshing manner, the rotating shaft is fixedly arranged in a mounting hole of the second bevel gear, the rotating block is uniformly and fixedly connected at the upper end part of the rotating shaft, the straight groove is uniformly arranged on the outer wall of the rotating block along the circumferential direction, the rotating rod is fixedly connected between the side walls of the straight groove, the rotating rod is rotatably connected with the connecting block, and the transverse rod is uniformly and fixedly arranged on the inner wall of the incubator along the circumferential direction, the inner end of the cross rod is fixedly connected with an arc-shaped plate, and the mounting structure rotates along with the rotating block to realize periodic contact with the top of the arc-shaped plate.
Furthermore, when the bottom of the mounting structure is in contact with the arc-shaped plate, the connecting block is pushed to rotate upwards along the rotating rod, and the mounting structure slides through the top point of the arc-shaped plate and then moves downwards.
Furthermore, when the mounting structure is not in contact with the arc-shaped plate, the bottom of the connecting block is in contact with the bottom of the straight groove in an attaching mode.
Furthermore, the oxygen suppliment heating structure includes muffler, oxygen hose, control valve, intake pipe, electric fan and annular spray tube, electric fan's output fixedly connected with intake pipe, the horizontal position fixed mounting of intake pipe has the oxygen hose, oxygen hose fixed mounting has the control valve, the end of giving vent to anger of intake pipe runs through incubator back fixedly connected with annular spray tube, electric fan's input fixedly connected with muffler, muffler fixed mounting is in the top straight hole of incubator.
Further, the annular nozzle is arranged below the rotating block at the lowest end.
Furthermore, the controller is electrically connected with the control valve, the electric heating fan, the temperature sensor and the oxygen sensor, the electromagnetic valve is selected for the control valve, the temperature sensor monitors the temperature in the incubator, the oxygen sensor monitors the oxygen concentration in the incubator, and the controller controls the control valve and the electric heating fan according to the monitoring data of the temperature sensor and the oxygen sensor.
Advantageous effects
Compared with the known public technology, the technical scheme provided by the invention has the following beneficial effects:
1. according to the pushing block of the sliding installation structure, the pushing block drives the curved clamping plate to move in the movable groove, then the culture dish is placed in the jack, the pushing block is loosened, the curved clamping plate is pushed to move by the restoring force of the second tension spring, the arc-shaped part of the curved clamping plate is matched with the jack to achieve fixed clamping of the culture dish, the culture dish is conveniently and fixedly installed, then the bolt is pulled outwards, the installation plate is inserted into the cross-shaped installation groove of the installation transverse plate, then the bolt is loosened, the restoring force of the first tension spring drives the bolt to be inserted into the bolt hole, the bolt achieves limiting of the installation plate, the installation plate containing multiple groups of culture dishes is conveniently assembled and disassembled in the installation transverse plate, a worker does not need to perform one group of installation in a narrow operation hole, and actual operation of the worker is facilitated.
2. According to the invention, the driving motor drives the first bevel gear to rotate, the first bevel gear drives the second bevel gear to rotate, the second bevel gear drives the rotating shaft to rotate, the rotating shaft drives the rotating block to rotate, the rotating block drives the connecting block to rotate through the rotating rod, the connecting block drives the mounting structure provided with the culture dish to rotate, the mounting structure is periodically contacted with the arc plate when rotating, the arc plate pushes the mounting structure to shake up and down, the shaken mounting structure is beneficial to movement of spores in the culture dish, so that the growth space between the spores in the culture dish is amplified, the spores are in a loose state during proliferation, mutual extrusion is avoided, the rapid growth of the spores is facilitated, the culture period is shortened, and the practical culture is facilitated.
3. The temperature sensor monitors the temperature in the incubator, the oxygen sensor monitors the oxygen concentration in the incubator, the controller controls the control valve of the oxygen supply heating structure and the electric heating fan according to the monitoring data of the temperature sensor and the oxygen sensor, the controller starts the control valve and the fan of the electric heating fan when the oxygen concentration in the incubator does not meet the requirement, the oxygen in the oxygen pipe enters the air inlet pipe, and the fan of the electric heating fan drives the oxygen to enter the incubator, so that the oxygen supply treatment is convenient; the temperature of incubator is less than when requiring the value controller start electric fan's fan and heating wire to control electric fan's hot-air temperature, in the hot-air passes through the intake pipe entering incubator, conveniently carries out temperature control, and simultaneously, the hot-air after the use passes through the muffler and recycles in getting into electric fan, and is energy-concerving and environment-protective.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.
FIG. 1 is a schematic view of the main structure of the present invention;
FIG. 2 is a structural elevation view of the present invention;
FIG. 3 is a right side view of the structure of the present invention;
FIG. 4 is a sectional view of an incubator and its connecting structure according to the present invention;
FIG. 5 is a cross sectional view of the mounting plate and its attachment structure of the present invention;
FIG. 6 is a sectional view of a connecting block and a connecting structure thereof according to the present invention;
FIG. 7 is an enlarged view of the structure at A of FIG. 5 according to the present invention;
FIG. 8 is an enlarged view of the structure at B of FIG. 5 according to the present invention;
FIG. 9 is an enlarged view of the structure of FIG. 6 at C according to the present invention;
FIG. 10 is a schematic view of a cross bar and its connection structure of the present invention;
the reference numerals in the drawings denote: 1. an incubator; 2. a support plate; 3. a controller; 4. pushing the plate; 5. an arc-shaped baffle plate; 6. a dithering structure; 61. a Z-shaped support frame; 62. a drive motor; 63. a first bevel gear; 64. a second bevel gear; 65. a rotating shaft; 66. connecting blocks; 67. rotating the block; 68. a cross bar; 69. an arc-shaped plate; 610. rotating the rod; 611. a straight groove; 7. a mounting structure; 71. mounting a plate; 72. a first tension spring; 73. installing a transverse plate; 74. a cross mounting groove; 75. a jack; 76. a bolt; 77. a curved splint; 78. a second tension spring; 79. a movable groove; 710. a pin hole; 711. a sliding groove; 712. a pushing block; 8. a glass window; 9. an arc-shaped guide rail; 10. an oxygen supply heating structure; 101. an air return pipe; 102. an oxygen tube; 103. a control valve; 104. an air inlet pipe; 105. an electric heating fan; 106. an annular nozzle; 11. a temperature sensor; 12. an oxygen sensor.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be further described with reference to the following examples.
Example 1
As shown in fig. 1, 3, 4, 5, 6, 7 and 10, the microbial spore culturing apparatus comprises an incubator 1 and a controller 3, wherein a supporting plate 2 is fixedly connected to the side wall of the incubator 1, the controller 3 is fixedly installed on the supporting plate 2, arc guide rails 9 are respectively arranged at the upper end and the lower end of an operating hole formed in the incubator 1, the arc guide rails 9 are movably connected with arc baffles 5 through arc grooves formed in the arc grooves, glass windows 8 are fixedly connected in through holes of the arc baffles 5, so that the specific conditions in the incubator 1 are facilitated, sealing rings in sealing contact with the incubator 1 are fixedly connected to the inner walls of the arc baffles 5, the incubator 1 and the arc baffles 5 are ensured to be in sealing connection, and a push plate 4 is connected to the left side of the outer ends of the arc baffles 5;
the culture box 1 is internally provided with a shaking structure 6 for shaking the culture dish, the shaking structure 6 is also provided with a mounting structure 7 for mounting the culture dish, the shaking structure 6 comprises a Z-shaped support frame 61, a driving motor 62, a first bevel gear 63, a second bevel gear 64, a rotating shaft 65, a connecting block 66, a rotating block 67, a cross bar 68, an arc-shaped plate 69, a rotating rod 610 and a straight groove 611, the Z-shaped support frame 61 is fixedly arranged at the bottom of the culture box 1, the driving motor 62 is fixedly arranged on the Z-shaped support frame 61, the output end of the driving motor 62 is fixedly connected with the first bevel gear 63, the first bevel gear 63 is in meshing connection with the second bevel gear 64, the rotating shaft 65 is fixedly arranged in a mounting hole of the second bevel gear 64, the inner bottom of the culture box 1 is rotatably connected with the rotating shaft 65 through a bearing which is fixedly connected, and the rotating block 67 is uniformly and fixedly connected at the upper end part of the rotating shaft 65, straight grooves 611 are uniformly formed in the outer wall of the rotating block 67 along the circumferential direction, a rotating rod 610 is fixedly connected between the side walls of the straight grooves 611, the rotating rod 610 is rotatably connected with a connecting block 66, the cross rod 68 is uniformly and fixedly installed on the inner wall of the incubator 1 along the circumferential direction, an arc-shaped plate 69 is fixedly connected to the inner end of the cross rod 68, the mounting structure 7 rotates along with the rotating block 67 to realize periodic contact with the top of the arc-shaped plate 69, the connecting block 66 is pushed to rotate upwards along the rotating rod 610 when the bottom of the mounting structure 7 is in contact with the arc-shaped plate 69, the mounting structure 7 slides over the top of the arc-shaped plate 69 to move downwards, and the bottom of the connecting block 66 is in contact with the bottom of the straight grooves 611 when the mounting structure 7 is not in contact with the arc-shaped plate 69;
the driving motor 62 drives the first bevel gear 63 to rotate, the first bevel gear 63 drives the second bevel gear 64 to rotate, the second bevel gear 64 drives the rotating shaft 65 to rotate, the rotating shaft 65 drives the rotating block 67 to rotate, the rotating block 67 drives the connecting block 66 to rotate through the rotating rod 610, the connecting block 66 drives the mounting structure 7 provided with the culture dish to rotate, the mounting structure 7 is in periodic contact with the arc plate 69 when rotating, the arc plate 69 pushes the mounting structure 7 to shake up and down, the shaken mounting structure 7 is beneficial to movement of spores in the culture dish, so that growth space among the spores in the culture dish is amplified, the spores are in a loose state during spore multiplication, mutual extrusion is avoided, rapid growth of the spores is facilitated, the culture period is shortened, and actual culture is facilitated;
the inner bottom of the incubator 1 is connected with an oxygen supply heating structure 10, and the side wall of the incubator 1 is fixedly connected with a temperature sensor 11 and an oxygen sensor 12.
Example 2
Example 2 is a further modification to example 1.
As shown in fig. 1, 3, 4, the oxygen supply heating structure 10 includes an air return pipe 101, an oxygen pipe 102, a control valve 103, an air inlet pipe 104, an electric heating fan 105 and an annular nozzle 106, wherein the output end of the electric heating fan 105 is fixedly connected with the air inlet pipe 104, the transverse part of the air inlet pipe 104 is fixedly provided with the oxygen pipe 102, the oxygen pipe 102 is fixedly provided with the control valve 103, the air outlet end of the air inlet pipe 104 penetrates through the incubator 1 and is fixedly connected with the annular nozzle 106, the input end of the electric heating fan 105 is fixedly connected with the air return pipe 101, the air return pipe 101 is fixedly arranged in a straight hole at the top of the incubator 1, the annular nozzle 106 is arranged below the rotating block 67 at the lowest end, the controller 3 is electrically connected with the control valve 103, the electric heating fan 105, the temperature sensor 11 and the oxygen sensor 12, the control valve 103 is an electromagnetic valve, the temperature sensor 11 monitors the temperature in the incubator 1, the oxygen sensor 12 monitors the oxygen concentration in the incubator 1, the controller 3 controls the control valve 103 and the electric heating fan 105 according to the monitoring data of the temperature sensor 11 and the oxygen sensor 12;
the temperature sensor 11 monitors the temperature in the incubator 1, the oxygen sensor 12 monitors the oxygen concentration in the incubator 1, the controller 3 controls the control valve 103 and the electric heating fan 105 of the oxygen supply heating structure 10 according to the monitoring data of the temperature sensor 11 and the oxygen sensor 12, the controller 3 starts the control valve 103 and the electric heating fan 105 when the oxygen concentration in the incubator 1 does not meet the requirement, the oxygen tube 102 is connected with the oxygen bottle, the oxygen in the oxygen tube 102 enters the air inlet tube 104, and the fan of the electric heating fan 105 drives the oxygen to enter the incubator 1, so that the oxygen supply treatment is convenient; when the temperature of the incubator 1 is lower than a required value, the controller 3 starts the fan and the heating wires of the electric heating fan 105 and controls the temperature of hot air of the electric heating fan 105, the hot air enters the incubator 1 through the air inlet pipe 104, temperature control is convenient to carry out, and meanwhile, the used hot air is recycled in the electric heating fan 105 through the air return pipe 101, so that the incubator is energy-saving and environment-friendly.
Example 3
Example 3 is a further modification to example 1.
As shown in fig. 2, 3, 4, 5, 7, 8 and 9, the mounting structure 7 includes a mounting plate 71, a first tension spring 72, a mounting transverse plate 73, a cross mounting groove 74, a plug 75, a plug 76, a curved clamping plate 77, a second tension spring 78, a movable groove 79, a plug hole 710, a sliding groove 711 and a pushing block 712, wherein the inner end of the mounting transverse plate 73 is fixedly connected with the outer end of the connecting block 66, the outer end side wall of the mounting transverse plate 73 is fixedly connected with the first tension spring 72, the outer end of the first tension spring 72 is fixedly connected with the plug 76, the mounting transverse plate 73 is provided with the cross mounting groove 74, the mounting transverse plate 73 is attached to and slidably connected with the mounting plate 71 through the cross mounting groove 74, the top of the mounting plate 71 is uniformly provided with the plug 75, a culture dish is inserted into the plug 75, the mounting plate 71 is provided with the plug hole 710 matched with the plug 76 on one side of the plug 75, the mounting plate 71 is provided with the movable groove 79 on the other side of the plug 75, the side wall of the movable groove 79 is uniformly and fixedly connected with a second tension spring 78, the inner end of the second tension spring 78 is fixedly connected with a curved splint 77, the arc part of the curved splint 77 moves in the insertion hole 75, the top of the straight part of the curved splint 77 is fixedly connected with a pushing block 712, the mounting plate 71 is provided with a sliding groove 711 matched with the pushing block 712, the sliding groove 711 is arranged between the two groups of insertion holes 75, and the sliding groove 711 is arranged at the middle end of the top of the mounting plate 71;
sliding mounting structure 7 promotes piece 712, it moves in movable groove 79 to promote piece 712 to drive bent shape splint 77, place the culture dish in jack 75 again, loosen and promote piece 712, second extension spring 78 restores force and promotes bent shape splint 77 and remove, the fixed centre gripping of culture dish is realized to bent shape splint 77's arc position and the cooperation of jack 75, make things convenient for culture dish fixed mounting, then outwards spur bolt 76, insert mounting panel 71 in the cross mounting groove 74 of installation diaphragm 73 again, loosen bolt 76 again, the restoring force of first extension spring 72 drives bolt 76 and inserts in bolt hole 710, bolt 76 realizes spacing to mounting panel 71, the mounting panel 71 loading and unloading that conveniently contains the multiunit culture dish is in installation diaphragm 73, need not the staff and carry out a set of installation in narrow and small operating hole, do benefit to staff's actual operation.
When the culture dish fixing device is used, the pushing block 712 of the sliding installation structure 7 drives the curved clamping plate 77 to move in the movable groove 79, a culture dish is placed in the jack 75, the pushing block 712 is loosened, the curved clamping plate 77 is pushed to move by the restoring force of the second tension spring 78, the arc part of the curved clamping plate 77 is matched with the jack 75 to realize the fixed clamping of the culture dish, the culture dish is convenient to fix and install, the bolt 76 is pulled outwards, the installation plate 71 is inserted into the cross installation groove 74 of the installation transverse plate 73, the bolt 76 is loosened, the bolt 76 is driven by the restoring force of the first tension spring 72 to be inserted into the bolt hole 710, the bolt 76 realizes the limiting of the installation plate 71, the installation plate 71 containing multiple groups of culture dishes is convenient to be assembled and disassembled in the installation transverse plate 73, a group of installation in a narrow operation hole is not needed by a worker, the actual operation of the worker is facilitated, the operation hole of the incubator 1 is sealed by the arc baffle 5, the controller 3 starts the driving motor 62, the driving motor 62 drives the first bevel gear 63 to rotate, the first bevel gear 63 drives the second bevel gear 64 to rotate, the second bevel gear 64 drives the rotating shaft 65 to rotate, the rotating shaft 65 drives the rotating block 67 to rotate, the rotating block 67 drives the connecting block 66 to rotate through the rotating shaft 610, the connecting block 66 drives the mounting structure 7 provided with the culture dish to rotate, the mounting structure 7 periodically contacts with the arc plate 69 when rotating, the arc plate 69 pushes the mounting structure 7 to shake up and down, the shaken mounting structure 7 is beneficial to movement of spores in the culture dish, so that growth space among the spores in the culture dish is amplified, the spores are in a loose state during proliferation, mutual extrusion does not exist, is beneficial to rapid growth of the spores, shortens the culture period, and is beneficial to actual culture; the temperature sensor 11 monitors the temperature in the incubator 1, the oxygen sensor 12 monitors the oxygen concentration in the incubator 1, the controller 3 controls the control valve 103 and the electric heating fan 105 of the oxygen supply heating structure 10 according to the monitoring data of the temperature sensor 11 and the oxygen sensor 12, the controller 3 starts the control valve 103 and the fan of the electric heating fan 105 when the oxygen concentration in the incubator 1 does not meet the requirement, the oxygen in the oxygen pipe 102 enters the air inlet pipe 104, and the fan of the electric heating fan 105 drives the oxygen to enter the incubator 1, so that the oxygen supply treatment is facilitated; when the temperature of the incubator 1 is lower than a required value, the controller 3 starts the fan and the heating wires of the electric heating fan 105 and controls the temperature of hot air of the electric heating fan 105, the hot air enters the incubator 1 through the air inlet pipe 104, temperature control is convenient to carry out, and meanwhile, the used hot air is recycled in the electric heating fan 105 through the air return pipe 101, so that the incubator is energy-saving and environment-friendly.
Wherein, reverse slip promotes piece 712 again, promotes piece 712 and drives curved splint 77 and the separation of culture dish, and the culture dish moves about in jack 75, makes things convenient for the cultivation to take out.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (9)
1. A microbial spore's cultivation equipment, includes incubator (1) and controller (3), its characterized in that: lateral wall fixedly connected with backup pad (2) of incubator (1), backup pad (2) fixed mounting has controller (3), the lower extreme all is provided with arc guide rail (9) on the handle hole that incubator (1) was seted up, arc guide rail (9) have cowl (5) through the arc wall swing joint who sets up, be provided with shake structure (6) that are used for the culture dish shake in incubator (1), the interior bottom connection of incubator (1) has oxygen suppliment heating structure (10), the lateral wall fixedly connected with temperature-sensing ware (11) and the oxygen inductor (12) of incubator (1).
2. The microbial spore cultivation apparatus according to claim 1, wherein a glass window (8) is fixedly connected in the through hole of the arc baffle plate (5), a sealing ring which is in sealing contact with the incubator (1) is fixedly connected on the inner wall of the arc baffle plate (5), and a push plate (4) is connected on the left side of the outer end of the arc baffle plate (5).
3. Apparatus for the cultivation of microbial spores according to claim 2, characterized in that the shaking structure (6) is further provided with a mounting structure (7) for the culture dish mounting.
4. The microbial spore cultivation apparatus according to claim 3, wherein the shaking structure (6) comprises a Z-shaped support frame (61), a driving motor (62), a first bevel gear (63), a second bevel gear (64), a rotating shaft (65), a connecting block (66), a rotating block (67), a cross bar (68), an arc-shaped plate (69), a rotating rod (610) and a straight groove (611), the Z-shaped support frame (61) is fixedly installed at the bottom of the incubator (1), the driving motor (62) is fixedly installed on the Z-shaped support frame (61), the output end of the driving motor (62) is fixedly connected with the first bevel gear (63), the first bevel gear (63) is in meshing connection with the second bevel gear (64), the rotating shaft (65) is fixedly installed in the installation hole of the second bevel gear (64), and the rotating block (67) is uniformly and fixedly connected with the upper end part of the rotating shaft (65), straight flute (611) have evenly been seted up along the circumferencial direction to the outer wall of turning block (67), fixedly connected with dwang (610) between the lateral wall of straight flute (611), dwang (610) rotate and are connected with connecting block (66), horizontal pole (68) are along the even fixed mounting of circumferencial direction at the inner wall of incubator (1), the inboard end fixedly connected with arc (69) of horizontal pole (68), mounting structure (7) are followed turning block (67) and are rotated the top periodic contact that realizes with arc (69).
5. The apparatus for cultivating microbial spores, according to claim 4, wherein the bottom of the mounting structure (7) is in contact with the arc-shaped plate (69) to push the connecting block (66) to rotate upward along the rotating rod (610), and the mounting structure (7) slides over the apex of the arc-shaped plate (69) and moves downward.
6. The apparatus for the cultivation of microbial spores according to claim 5, wherein the bottom of the connecting block (66) is in abutting contact with the bottom of the straight groove (611) when the mounting structure (7) is not in contact with the curved plate (69).
7. The microbial spore cultivation apparatus according to any one of claims 1 to 6, wherein the oxygen supply heating structure (10) comprises an air return pipe (101), an oxygen pipe (102), a control valve (103), an air inlet pipe (104), an electric heating fan (105) and a ring-shaped spray pipe (106), the air inlet pipe (104) is fixedly connected to the output end of the electric heating fan (105), the oxygen pipe (102) is fixedly installed at the transverse part of the air inlet pipe (104), the control valve (103) is fixedly installed on the oxygen pipe (102), the ring-shaped spray pipe (106) is fixedly connected to the rear part of the incubator (1) through the air outlet end of the air inlet pipe (104), the air return pipe (101) is fixedly connected to the input end of the electric heating fan (105), and the air return pipe (101) is fixedly installed in a straight hole at the top of the incubator (1).
8. The apparatus for culturing microbial spores according to claim 7, wherein the annular nozzle (106) is disposed below the lowermost turning block (67).
9. The apparatus for culturing microbial spores, according to claim 7, wherein the controller (3) is electrically connected with the control valve (103), the electric heating fan (105), the temperature sensor (11) and the oxygen sensor (12), the control valve (103) is a solenoid valve, the temperature sensor (11) monitors the temperature in the incubator (1), the oxygen sensor (12) monitors the oxygen concentration in the incubator (1), and the controller (3) controls the control valve (103) and the electric heating fan (105) according to the monitoring data of the temperature sensor (11) and the oxygen sensor (12).
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