CN113198833A - Petroleum pollution remediation method, petroleum hydrocarbon degradation agent and flora - Google Patents

Petroleum pollution remediation method, petroleum hydrocarbon degradation agent and flora Download PDF

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CN113198833A
CN113198833A CN202110282740.9A CN202110282740A CN113198833A CN 113198833 A CN113198833 A CN 113198833A CN 202110282740 A CN202110282740 A CN 202110282740A CN 113198833 A CN113198833 A CN 113198833A
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petroleum
injecting
land
petroleum hydrocarbon
polluted
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李璐璐
王利刚
李洋铭
翟勇
温焕君
高悦
王文文
张书畅
杨慧萍
徐文博
尹伟
隋宗津
刘登峰
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China National Petroleum Corp
China Kunlun Contracting and Engineering Corp
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China National Petroleum Corp
China Kunlun Contracting and Engineering Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/08—Reclamation of contaminated soil chemically
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • C09K17/48—Organic compounds mixed with inorganic active ingredients, e.g. polymerisation catalysts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C2101/00—In situ

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Abstract

The invention discloses a petroleum pollution remediation method, a petroleum hydrocarbon degradation agent and a flora. The petroleum pollution remediation method comprises the following steps: injecting petroleum hydrocarbon oxidation degradation agent into petroleum polluted land; and after the condition is determined to reach the set condition, injecting the petroleum hydrocarbon degrading bacteria liquid into the oxidized petroleum-polluted land. Can comprehensively utilize chemical and microbial methods to realize deep degradation of petroleum hydrocarbons in petroleum-polluted plots, and has the advantages of low energy consumption, low cost and no damage to the environment.

Description

Petroleum pollution remediation method, petroleum hydrocarbon degradation agent and flora
Technical Field
The invention relates to the technical field of environmental pollution treatment, in particular to a petroleum pollution remediation method, a petroleum hydrocarbon degrading agent and a flora.
Background
Petroleum is one of the major energy sources of modern economy. However, various leakage accidents occur in the exploration, exploitation, storage, transportation and use processes of oil fields, and the solid waste storage method in the production process is improper, so that the oil pollution of soil or water bodies is caused to different degrees. The soil structure can be damaged by petroleum pollution, and the soil pores are blocked by the petroleum pollutants, so that the water permeability and the air permeability of the soil are reduced, the carbon-nitrogen ratio and the carbon-phosphorus ratio of soil organic matters are changed, the microbial quantity and community structure of the soil are changed, and the micro-ecological environment of the soil is damaged; meanwhile, the petroleum pollutants in the soil are carried by rainwater to pollute surface water, which affects water safety and crop safety, or underground water is polluted by downward leakage, and human health risks are generated through drinking water sources. The petroleum pollution has large concealment, long latency, wide related range, difficult treatment and increasingly obvious harm, and becomes an environmental problem which cannot be ignored.
The conventional petroleum pollution remediation methods include physical treatment, chemical treatment, and microbial treatment.
The chemical treatment method can quickly remove petroleum hydrocarbon pollutants remained in the soil at a low cost, but the treatment effect of the soil with severe petroleum pollution is limited, for example, the treatment effect of polycyclic aromatic hydrocarbons is not ideal, and the problems of damaging the structural property of the soil, greatly increasing the construction cost and the like exist by adding a large amount of oxidizing agents for many times. The microbial treatment method is an important method for removing petroleum hydrocarbon pollution containing polycyclic aromatic hydrocarbon, has the advantages of thorough degradation, simple process operation, environmental friendliness and the like, and simultaneously has the problems of poor treatment effect on high-concentration pollution, overlong treatment period on high-molecular-weight polycyclic aromatic hydrocarbon and the like. Therefore, both the chemical treatment method and the microbial treatment method have the problems of poor treatment effect on high-concentration and high-molecular-weight petroleum hydrocarbon pollutants, long period and high cost.
Disclosure of Invention
In view of the above problems, the present invention has been made to provide a method for remediating petroleum pollution, a petroleum hydrocarbon degrading agent, and a bacterial population that overcome or at least partially solve the above problems.
In a first aspect, an embodiment of the present invention provides a method for remediating petroleum pollution, including:
injecting petroleum hydrocarbon oxidation degradation agent into petroleum polluted land;
and after the set conditions are determined to be met, injecting the petroleum hydrocarbon degrading flora into the oxidized petroleum-polluted land.
In a second aspect, embodiments of the present invention provide a petroleum hydrocarbon degrading agent, including a catalyst and an oxidizing agent;
the catalyst comprises a ferrous sulfate and citric acid mixed solution;
the oxidizing agent is a hydrogen peroxide solution.
In a third aspect, embodiments of the present invention provide a petroleum hydrocarbon degrading bacterial population, which is a mixed bacterial population including acinetobacter calcoaceticus and petroleum hydrocarbon degrading bacteria.
The technical scheme provided by the embodiment of the invention has the beneficial effects that at least:
(1) according to the petroleum pollution remediation method provided by the embodiment of the invention, a petroleum hydrocarbon oxidation degradation reagent is injected into a petroleum-polluted land; and after the condition is determined to reach the set condition, injecting the petroleum hydrocarbon degrading bacteria liquid into the oxidized petroleum-polluted land. The deep degradation of petroleum hydrocarbon in the petroleum-polluted land can be realized by comprehensively utilizing chemical and microbial methods, the degradation of petroleum hydrocarbon pollutants comprising polycyclic aromatic hydrocarbon is thorough, and the limitation of a single repair technology is overcome; and by the methods of chemical oxidative degradation and microbial degradation, secondary pollution is not caused, and the method is environment-friendly.
(2) According to the petroleum pollution remediation method provided by the embodiment of the invention, a large amount of oxygen is generated through an early oxidation reaction and is left in soil, and the oxygen can be used as an electron acceptor of aerobic microorganisms, so that the microbial degradation speed is increased; meanwhile, the temperature of the petroleum-polluted land is raised by the early-stage oxidation reaction, a proper temperature is provided for the metabolism of the petroleum hydrocarbon of the microorganism, and the requirement of an external heat source is reduced, so that the energy consumption is reduced and the cost is reduced while the decomposition of the petroleum hydrocarbon is more thorough; and the invention does not need large-scale repair equipment and higher repair cost.
(3) The petroleum hydrocarbon degradation agent provided by the embodiment of the invention comprises a catalyst and an oxidation agent; the catalyst comprises ferrous sulfate and citric acid mixed solution; the oxidizing agent is a hydrogen peroxide solution. Citric acid is added to adjust the pH value of the reaction system, and iron ions can be chelated to form a high-density complex, so that the catalytic activity of the ferrous sulfate is better; hydrogen peroxide by Fe2+Catalytic decomposition productsThe raw HO-, HO-can generate oxidation reaction with petroleum pollutants to lead macromolecules in the raw HO-, HO-and petroleum pollutants to be decomposed into small molecular organic substances capable of being biochemically degraded or directly mineralized into CO2、H2O and the like, thereby achieving the purpose of degrading petroleum hydrocarbon in the soil, the underground water body and other plots.
(4) The petroleum hydrocarbon degrading flora provided by the embodiment of the invention is a mixed flora comprising acinetobacter calcoaceticus and petroleum hydrocarbon degrading bacteria. The acinetobacter calcoaceticus has strong petroleum emulsifying capacity, so that petroleum pollutants are stripped from the surface of soil particles and are more easily dissolved in water, thereby increasing the contact chance of petroleum hydrocarbon degrading bacteria and the petroleum pollutants and improving the decomposition efficiency; petroleum hydrocarbon degrading bacteria can convert petroleum pollutants including polycyclic aromatic hydrocarbons into H through metabolism of petroleum hydrocarbon degrading bacteria2O and CO2So that the petroleum pollutants are converted and removed in the metabolic circulation of the flora. The petroleum hydrocarbon degrading flora provided by the embodiment of the invention can grow and reproduce by using petroleum as a unique carbon source without adding another carbon source.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a flow chart of a method for remediating petroleum pollution according to a first embodiment of the present invention.
Detailed Description
Exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the invention are shown in the drawings, it should be understood that the invention can be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
In order to solve the problems of incomplete petroleum pollution remediation and long remediation period in the prior art, the embodiment of the invention provides a petroleum pollution remediation method, a petroleum hydrocarbon degradation medicament and a flora, which can realize deep degradation of petroleum hydrocarbon in a petroleum-polluted land by comprehensively utilizing chemical and microbial methods, and has the advantages of low energy consumption, low cost and no damage to the environment.
Example one
The embodiment of the invention provides a method for restoring petroleum pollution, the flow of which is shown in figure 1, and the method comprises the following steps:
step S11: injecting petroleum hydrocarbon oxidation degradation agent into petroleum polluted land.
The method specifically comprises the steps of firstly, injecting a mixed solution of ferrous sulfate and citric acid into a petroleum-polluted land; and after the pH value of the petroleum-polluted land is determined to reach a first set range, injecting a hydrogen peroxide solution into the petroleum-polluted land.
Fe is required for the oxidative decomposition of hydrogen peroxide2+Catalytic action of (1), and Fe2+The catalytic action of the catalyst is better at a proper pH value, and the citric acid can reduce the pH value of the reaction system, so that the catalytic solution (ferrous sulfate and citric acid mixed solution) is injected before the oxidizing solution (hydrogen peroxide solution) is injected.
Preferably, the mixed solution of ferrous sulfate and citric acid is injected into the petroleum-polluted land, after the pH value of the petroleum-polluted land is determined to be reduced to 3-5, the hydrogen peroxide solution is injected into the petroleum-polluted land.
Since the reaction speed of hydrogen peroxide to oxidize and decompose petroleum hydrocarbon is fast, in order to reduce the consumption of hydrogen peroxide and simultaneously make the reaction more uniform, the speed of the oxidation reaction needs to be controlled, and in one embodiment, the injection of the hydrogen peroxide solution into the petroleum-polluted land can specifically comprise the injection of the mixed solution of hydrogen peroxide and the oxidation speed regulator into the petroleum-polluted land.
In one embodiment, the molar ratio of ferrous sulfate to hydrogen peroxide injected into the petroleum-contaminated land is 1: 100-1: 10. other agent proportions can be determined experimentally based on the nature of the petroleum-contaminated land and the reaction rate requirements.
In the petroleum hydrocarbon oxidative degradation medicament for petroleum pollution remediation, design parameters such as the dosage, the injection speed and the like of the medicament are based on specific pollution conditions, and the reaction time is determined according to a real-time monitoring result.
The land polluted by the petroleum is mainly soil or underground water.
Step S12: and after the condition is determined to reach the set condition, injecting the petroleum hydrocarbon degrading bacteria liquid into the oxidized petroleum-polluted land.
Specifically, the set condition may be that the decrease range of the concentration of the contaminant in the petroleum-contaminated land is determined to be lower than the set range, and the concentration of the hydrogen peroxide is determined to be lower than the set concentration.
For example, it may be that, after the hydrogen peroxide oxidizing solution is injected, the petroleum-contaminated land is monitored for changes in the concentration of benzene representing the contaminant; stopping injecting the oxidizing solution after the pollution concentration is reduced and stabilized (the reduction amplitude of the pollution concentration is lower than a set amplitude); after the concentration of hydrogen peroxide is continuously monitored and confirmed to be lower than the set concentration, for example, may be lower than 0.01%, the oxidation reaction is judged to be almost completed, and the petroleum hydrocarbon degrading bacteria liquid may be injected.
In one embodiment, the injecting the petroleum hydrocarbon-degrading bacteria solution into the oxidized petroleum-contaminated land may specifically include injecting a mixed bacteria solution containing acinetobacter calcoaceticus and petroleum hydrocarbon-degrading bacteria into the oxidized petroleum-contaminated land.
In one embodiment, injecting the petroleum hydrocarbon-degrading bacterial liquid into the oxidized petroleum-contaminated land may specifically include injecting the liquid containing NH4NO3、K2HPO4And KH2PO4The nutrient solution and the petroleum hydrocarbon degrading bacteria liquid are injected into the oxidized petroleum-polluted land. Preferably, the nitrogen-phosphorus ratio in the nutrient solution is 10:1, and the pH value of the nutrient solution is 6.3-6.5, so that the decomposition speed of the flora is better.
According to the petroleum pollution remediation method provided by the embodiment of the invention, a petroleum hydrocarbon oxidation degradation reagent is injected into a petroleum-polluted land; and after the condition is determined to reach the set condition, injecting the petroleum hydrocarbon degrading bacteria liquid into the oxidized petroleum-polluted land. The deep degradation of petroleum hydrocarbon in the petroleum-polluted land can be realized by comprehensively utilizing chemical and microbial methods, the degradation of petroleum hydrocarbon pollutants comprising polycyclic aromatic hydrocarbon is thorough, and the limitation of a single repair technology is overcome; and by the methods of chemical oxidative degradation and microbial degradation, secondary pollution is not caused, and the method is environment-friendly.
A large amount of oxygen is generated through the early oxidation reaction and is remained in the soil, and the oxygen can be used as an electron acceptor of aerobic microorganisms, so that the degradation speed of the microorganisms is improved; meanwhile, the temperature of the petroleum-polluted land is raised by the early-stage oxidation reaction, a proper temperature is provided for the metabolism of the petroleum hydrocarbon of the microorganism, and the requirement of an external heat source is reduced, so that the energy consumption is reduced and the cost is reduced while the decomposition of the petroleum hydrocarbon is more thorough; and the invention does not need large-scale repair equipment and higher repair cost.
The petroleum-contaminated land is mainly soil or underground water, and may be in situ or ex situ.
According to the petroleum pollution remediation method, when the in-situ remediation process is used, the oxidative degradation agents or the degradation bacteria liquid are sequentially injected into the polluted soil or underground water through the injection well, and the arrangement scheme of the injection well is determined according to the pollution degree and the pollution range of the soil. When the ectopic repair process is used, the oxidative degradation medicament or the degradation bacteria liquid is sequentially injected into the polluted soil or underground water through the injection pipe, and the arrangement scheme of the injection pipe is determined according to the pollution degree and the pollution scale.
Example two
The second embodiment of the invention provides an application example of a petroleum-polluted soil remediation method.
The site is used for repairing and treating the pollution of part of underground water of a pollution source after the pollution source containing NAPL (petroleum naphthalene) of petroleum class is removed, the main pollutants are petroleum hydrocarbons such as benzene, ethylbenzene, xylene and the like, and the pollution range is 4000m2The range of the pollution depth isNear the underground water surface, an aeration zone with the thickness of 3-5 m and a diving aquifer are arranged, and the underground water restoration target is three standards of underground water quality, namely the benzene concentration is less than 0.12 mg/L.
Arranging injection wells according to pollution conditions, injecting a ferrous sulfate and citric acid mixed solution by adopting a pressure grouting pump, sampling and monitoring wells around the injection wells, and determining pH change and Fe2+After the concentration changes and the pH condition (pH value 4.5-5.5) of the oxidation reaction is reached and stabilized, injecting a mixed solution of hydrogen peroxide and a regulator (oxidation solution); continuously sampling and monitoring the concentration change of benzene representing the pollutant through a well, and stopping injecting the oxidant after the pollution concentration is reduced and stabilized; continuously monitoring to determine that the concentration of the hydrogen peroxide is lower than 0.01 percent, and then judging that the oxidation reaction is basically finished; then, sequentially injecting buffer/nutrient solution and microorganism mixed bacteria liquid for microorganism remediation, wherein the injected buffer/nutrient solution is composed of NH4NO3、K2HPO4And KH2PO4The composition (buffer solution N/P ratio is 9:1, pH value is 6.3), the mixed bacterial liquid comprises acinetobacter calcoaceticus and petroleum hydrocarbon degrading bacteria.
Monitoring the benzene concentration change of the monitoring well 1 in the repairing process, and observing the influence of natural degradation on the benzene concentration by using the benzene concentration change of the monitoring well 2 (which is positioned in a repairing area and only injecting an oxidizing solution around the monitoring well) as a contrast. The monitoring result before repair shows that the benzene concentration of the two monitoring wells is basically stabilized at 8-10 mg/L; after the oxidizing solution is injected, the benzene concentration is reduced by about 30 percent, and then the concentration has rebound phenomenon; after the mixed bacteria liquid is injected into the monitoring well 1 area, the benzene concentration is stably reduced, the microbial remediation effect is obvious, the concentration reaches below 1mg/L in 120 days, and the concentration reaches below 0.1mg/L in 180 days; and the benzene concentration of the monitoring well 2 is slowly reduced, the concentration is still more than 3mg/L in 150 days, then a method for removing pollution by injecting the oxidant for multiple times in the area of the monitoring well 2 is adopted, but the concentration rebound is obvious, and the benzene concentration is stabilized below 0.1mg/L only by injecting the oxidant for 4 times.
The results of the embodiment show that the oxidation and microorganism combined remediation method aims at alkaline underground water polluted by petroleum, the overall engineering cost is 2/3-1/2 of a simple oxidation technology, and meanwhile, the method has obvious advantages in the aspects of avoiding rebound of pollutant concentration after remediation, cost performance, high pollution removal rate and the like,
EXAMPLE III
The third embodiment of the invention provides an application example of another method for restoring petroleum-polluted soil.
The site pollution is lower polluted soil after solid waste containing oil sludge and waste residues is removed. The pH value of the soil is 7.9-8.6, and the main pollutants are petroleum hydrocarbons, including aromatic hydrocarbons such as benzene and toluene and polycyclic aromatic hydrocarbons such as naphthalene and benzopyrene. Polluted soil size 28000m3The concentration range of the petroleum hydrocarbon is 20900-25000mg/kg, the average is 23000mg/kg, wherein the maximum concentration of benzene is 8.7mg/kg, and the maximum concentration of naphthalene is 181 mg/kg; the repairing technology adopts an ectopic repairing method combining oxidation and microorganisms, and the repairing target is based on a construction land soil pollution risk screening guide value (secondary request opinion draft)]Sieving value for type II, petroleum hydrocarbon (C)10-C40) The concentration is lower than 4500mg/kg, the benzene concentration is lower than 4mg/kg, and the naphthalene concentration is lower than 70 mg/kg.
Arranging an anti-seepage hardened layer in an in-situ treatment area, digging polluted soil from the polluted area and conveying the polluted soil to the treatment area after constructing a percolate drainage channel, stacking the polluted soil in layers and arranging an injection horizontal pipe network, and adding a mixed solution of ferrous sulfate serving as a catalyst and citric acid serving as a pH regulator into a polluted soil pile by adopting a method of surface layer spraying and high-pressure grouting pump injection into the pipe network to adjust the pH value of the soil to be below 6.0; and then injecting a mixed solution (oxidizing solution) of hydrogen peroxide and a regulator into a pipe network through a high-pressure grouting pump, permeating the mixed solution into the polluted soil, and decomposing the pollutants into harmless water, carbon dioxide and inorganic ions by the chemical reaction of the hydrogen peroxide and the petroleum hydrocarbon pollutants under the action of a catalyst, so that the effect of decomposing the pollutants is achieved.
Periodically sampling and monitoring the treatment effect of the polluted soil, confirming that the concentration of the soil pollutants is rapidly reduced in the early stage, but the concentration is slowly reduced to about 15000mg/kg, starting to carry out microbial remediation, and adding mixed flora and buffer solution to the soil heap by adopting a method of surface layer spraying and high-pressure grouting pump injection into a pipe network, wherein the mixed flora comprises acinetobacter calcoaceticus and petroleum hydrocarbon degradationBacteria; the buffer comprises NH4NO3、K2HPO4And KH2PO4The buffer solution is adjusted to have a N/P ratio of 10:1 and a pH value of about 6.5.
Monitoring results show that after the mixed flora and the buffer solution are injected, the concentration of soil pollutants is reduced to be less than 8000mg/kg in 90 days, wherein the concentrations of benzene and naphthalene are reduced to be less than target values, and the concentration reaches 3500mg/kg in 180 days, so that the remediation target is achieved; in a control area, the soil concentration is slowly reduced, the concentration is still about 12000mg/kg in 180 days, then a method of continuously injecting an oxidant is adopted, the concentration reaches about 4000mg/kg in 240 days, and the concentrations of benzene and naphthalene reach the standard.
The results of the embodiment show that the oxidation and microorganism combined remediation method aims at petroleum-polluted alkaline soil, the overall engineering cost is 1/3-1/2 of that of a simple oxidation technology, and the remediation period is shortened. The method has obvious advantages in the aspects of cost performance, pollution removal rate and the like.
Based on the inventive concept of the present invention, the embodiment of the present invention further provides a petroleum hydrocarbon degradation agent, which comprises a catalyst and an oxidation agent; the catalyst comprises ferrous sulfate and citric acid mixed solution; the oxidizing agent is a hydrogen peroxide solution.
Citric acid is added to adjust the pH value of the reaction system, and iron ions can be chelated to form a high-density complex, so that the catalytic activity of the ferrous sulfate is better; hydrogen peroxide by Fe2+The HO-can be generated by catalytic decomposition, and can be oxidized with petroleum pollutants to lead macromolecules in the HO-to be decomposed into small molecular organic substances which can be biochemically degraded or directly mineralized into CO2、H2O and the like, thereby achieving the purpose of degrading petroleum hydrocarbon in the soil, the underground water body and other plots.
Preferably, the molar ratio of the ferrous sulfate to the hydrogen peroxide is 1: 100-1: 10.
based on the inventive concept of the present invention, the embodiment of the present invention further provides a petroleum hydrocarbon degrading flora, which is a mixed flora comprising acinetobacter calcoaceticus and petroleum hydrocarbon degrading bacteria.
The acinetobacter calcoaceticus has strong petroleum emulsifying capacity, so that petroleum pollutants are stripped from the surface of soil particles, andthe oil-water soluble organic fertilizer is easy to dissolve in water, so that the contact chance of petroleum hydrocarbon degrading bacteria and petroleum pollutants is increased, and the decomposition efficiency is improved; petroleum hydrocarbon degrading bacteria can convert petroleum pollutants including polycyclic aromatic hydrocarbons into H through metabolism of petroleum hydrocarbon degrading bacteria2O and CO2So that the petroleum pollutants are converted and removed in the metabolic circulation of the flora. The petroleum hydrocarbon degrading flora provided by the embodiment of the invention can grow and reproduce by using petroleum as a unique carbon source without adding another carbon source.
Preferably, the mixed flora is further supplemented with a composition comprising NH4NO3、K2HPO4And KH2PO4The nutrient solution of (1).
The nitrogen-phosphorus ratio in the nutrient solution is 10:1, and the pH value of the nutrient solution is 6.3-6.5.
In the foregoing detailed description, various features are grouped together in a single embodiment for the purpose of streamlining the disclosure. This method of disclosure is not to be interpreted as reflecting an intention that the claimed embodiments of the subject matter require more features than are expressly recited in each claim. Rather, as the following claims reflect, invention lies in less than all features of a single disclosed embodiment. Thus, the following claims are hereby expressly incorporated into the detailed description, with each claim standing on its own as a separate preferred embodiment of the invention.
What has been described above includes examples of one or more embodiments. It is, of course, not possible to describe every conceivable combination of components or methodologies for purposes of describing the aforementioned embodiments, but one of ordinary skill in the art may recognize that many further combinations and permutations of various embodiments are possible. Accordingly, the embodiments described herein are intended to embrace all such alterations, modifications and variations that fall within the scope of the appended claims. Furthermore, to the extent that the term "includes" is used in either the detailed description or the claims, such term is intended to be inclusive in a manner similar to the term "comprising" as "comprising" is interpreted when employed as a transitional word in a claim. Furthermore, any use of the term "or" in the specification of the claims is intended to mean a "non-exclusive or".

Claims (10)

1. A method for remediating petroleum pollution, comprising:
injecting petroleum hydrocarbon oxidation degradation agent into petroleum polluted land;
and after the condition is determined to reach the set condition, injecting the petroleum hydrocarbon degrading bacteria liquid into the oxidized petroleum-polluted land.
2. The method of claim 1, wherein injecting the petroleum hydrocarbon oxidative degradation agent into the petroleum-contaminated land comprises:
injecting the mixed solution of ferrous sulfate and citric acid into the petroleum-polluted land;
and after the pH value of the petroleum-polluted land is determined to reach a first set range, injecting a hydrogen peroxide solution into the petroleum-polluted land.
3. The method of claim 2, further comprising:
the molar ratio of ferrous sulfate to hydrogen peroxide injected into the petroleum-contaminated land is 1: 100-1: 10.
4. the method according to claim 2 or 3, wherein the injecting of the hydrogen peroxide solution into the petroleum-contaminated land comprises:
injecting the mixed solution of hydrogen peroxide and an oxidation rate regulator into the petroleum-polluted land.
5. The method of claim 1, wherein the injecting the petroleum hydrocarbon-degrading bacterial solution into the oxidized petroleum-contaminated land comprises:
injecting the mixed bacterial liquid containing the acinetobacter calcoaceticus and the petroleum hydrocarbon degrading bacteria into the oxidized petroleum-polluted land.
6. The method according to claim 1 or 5, wherein the injecting the petroleum hydrocarbon degrading bacterial liquid into the oxidized petroleum-contaminated land specifically comprises:
will contain NH4NO3、K2HPO4And KH2PO4The nutrient solution and the petroleum hydrocarbon degrading bacteria liquid are injected into the oxidized petroleum-polluted land.
7. The method according to claim 6, wherein the nitrogen-phosphorus ratio in the nutrient solution is 10:1, and the pH value of the nutrient solution is 6.3-6.5.
8. The method of claim 1, wherein the determining that the set condition is met specifically comprises:
and determining that the reduction amplitude of the concentration of the pollutants in the petroleum-polluted land is lower than a set amplitude, and the concentration of the hydrogen peroxide is lower than a set concentration.
9. A petroleum hydrocarbon degrading agent, which is characterized by comprising a catalyst and an oxidizing agent;
the catalyst comprises a ferrous sulfate and citric acid mixed solution;
the oxidizing agent is a hydrogen peroxide solution.
10. A petroleum hydrocarbon degrading flora, wherein the petroleum hydrocarbon degrading flora is a mixed flora comprising Acinetobacter calcoaceticus and petroleum hydrocarbon degrading bacteria.
CN202110282740.9A 2021-03-16 2021-03-16 Petroleum pollution remediation method, petroleum hydrocarbon degradation agent and flora Pending CN113198833A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115745193A (en) * 2022-11-15 2023-03-07 吉林大学 Composite medicament for promoting microorganisms to degrade petroleum hydrocarbon in underground water

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CN115745193B (en) * 2022-11-15 2024-05-28 吉林大学 Composite medicament for promoting microorganism to degrade petroleum hydrocarbon in underground water

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