CN113189327B - High-sensitivity detection test paper box and application thereof - Google Patents
High-sensitivity detection test paper box and application thereof Download PDFInfo
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- CN113189327B CN113189327B CN202110506363.2A CN202110506363A CN113189327B CN 113189327 B CN113189327 B CN 113189327B CN 202110506363 A CN202110506363 A CN 202110506363A CN 113189327 B CN113189327 B CN 113189327B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
Abstract
The invention discloses a high-sensitivity detection test paper box and application thereof, wherein the test paper strip adopts a Y-shaped structure formed by a detection end with a detection line and a quality control end with a quality control line, and a detection test paper box which is matched with the detection test paper box and comprises a quality control tank and a detection tank is arranged above the quality control tank and the detection tank, a pressing device with a part of adhesive absorbent paper is arranged above the quality control tank and the detection tank, so that whether the test paper strip is effective or not can be detected while a sample is detected, and the detection test paper strip can accurately detect the sample under the condition that the content of a marker to be detected in the sample is low, has extremely high detection sensitivity, and is easy to popularize and use in medical work or household detection.
Description
Technical Field
The invention belongs to the technical field of medical treatment, and particularly relates to a high-sensitivity detection test paper box and application thereof.
Background
Currently, a commercial detection test paper box adopts a double-antibody sandwich one-step method with colloidal gold coupled with an antibody as an indication mark to detect a marker to be detected in a sample. When the detection test paper is used for detection, a marker to be detected-antibody colloidal gold complex is formed when a detected sample passes through the colloidal gold-coupled antibody, the complex is continuously diffused, a double-antibody sandwich colloidal gold complex is formed when the detected sample reaches a detection line (the monoclonal antibody corresponding to the marker to be detected is coated), a color band is presented at the detection line, excessive colloidal gold antibody is continuously crawled, and a quality control line antibody (used for detecting whether the antibody is inactivated) forms a colloidal gold immune complex and presents the color band. However, due to the limitation of detection sensitivity, a more accurate detection result can be obtained only when the marker to be detected in the sample reaches a certain content. If the sample contains fewer markers to be detected, then an accurate detection result cannot be obtained.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a high-sensitivity detection test paper box and application thereof, solves the technical problems that the detection result is inaccurate or cannot be detected when a small amount of markers are contained in a sample, and reduces the detection limit.
The technical scheme of the invention is realized specifically as follows:
the utility model provides a high sensitivity detects test paper box, includes the storage box of placing the test paper, the storage box comprises test paper cassette and test paper cassette upper cover, the test paper cassette upper cover is provided with drop appearance mouth and slider, test paper cassette one end rotates and connects in the shell, the shell comprises shell base and shell upper cover, the shell upper cover is provided with hole one, hole two, hole three, drop appearance mouth and hole one cooperate the use, the slider passes hole two and slides in hole two and use, hole three cooperates with the detection pond and the quality control pond that the shell base inboard set up and uses;
the detection device is characterized in that a hole is formed between the detection tank and the quality control tank, two water-absorbing paper fixing frames B are arranged on a pressing rod of a pressing rod capable of moving downwards and arranged in the hole, part-adhered water-absorbing paper is arranged at the bottoms of the two water-absorbing paper fixing frames B, and part, which is not adhered, of the two water-absorbing paper and the water-absorbing paper fixing frames B is located above the detection tank and the quality control tank respectively.
The hole is internally provided with a flexible limiting clamp, the pressing rod is arranged in the flexible limiting clamp, the flexible limiting clamp is provided with a bulge, the pressing rod is provided with a plurality of grooves which are arranged up and down and matched with the bulge, and the bulge is in a state of entering any groove in the downward movement process of the pressing rod.
The pressing rod and the two absorbent paper fixing frames B form a pressing device. When the sliding block is stirred, the sliding block drives the test strip in the storage box to move to the position above the detection pool and the quality control pool and below the two water absorbing paper fixing frames B of the pressing device, at the moment, the non-adhered parts of the two water absorbing papers and the fixing frames B are respectively positioned above the detection pool and the quality control pool, and the test strip is at a certain distance from the two water absorbing paper fixing frames B above and from the detection pool and the quality control pool below, so that the test strip is prevented from being damaged due to friction when the sliding block is stirred, and besides, the tail end of the test strip is pressed tightly with the water absorbing papers; in addition, the bottom of the two absorbent paper fixing frames B are respectively provided with partially-adhered absorbent paper, namely: the absorbent paper is arranged at the bottom of the absorbent paper fixing frame B, one part of the absorbent paper is stuck at the bottom of the absorbent paper fixing frame B, and the other part of the absorbent paper is not stuck, so that the absorbent paper of the non-stuck part and the absorbent paper of the stuck part are on the same plane under the condition of drying, and when the absorbent paper is soaked, the absorbent paper of the non-stuck part is naturally bent under the action of gravity.
The storage box for placing the test paper strip consists of a test paper clamping seat and a test paper clamping seat upper cover, and preferably, the test paper clamping seat and the test paper clamping seat upper cover are buckled and disassembled; the shell consists of a shell base and a shell upper cover, and preferably, the shell base and the shell upper cover are buckled and detached.
The inner side of the shell base is provided with a water absorbing paper fixing frame A, the bottom of the shell base is stuck with water absorbing paper for absorbing silver nano particles-antibodies which are not combined with the quality control line/detection line after the sample is dripped, and meanwhile, the forward flowing effect of the sample and the marker to be detected-antibody-silver nano particles composite is promoted.
The shell base is provided with a rotating shaft which is used for rotating and connecting the test paper clamping seat, and the test paper strip in the storage box is driven to slide when the sliding block is stirred.
The test strip comprises a sample pad, a silver mark pad, an NC film and a plastic rubber plate, wherein the test strip is Y-shaped, and the tail end of the test strip is provided with a detection end with a detection line and a quality control end with a quality control line. The detection end with the detection line is used for detecting whether the sample contains a marker to be detected or not, and the quality control end with the quality control line is used for detecting whether the test paper strip contains silver nano-particles-antibodies or whether the silver nano-particles-antibodies fail or not. Therefore, whether the test strip fails or not can be detected while the marker to be detected is detected, detection and quality control are avoided, and detection time is saved. When the test strip is used, the test strip is slid to the detection pond and the quality control pond, and the detection end and the quality control end of the test strip are respectively positioned above the detection pond and the quality control pond.
The test paper strip is Y-shaped, is placed in the Y-shaped groove that sets up on the test paper cassette, and the length of Y-shaped test paper strip is greater than the length in Y-shaped groove, can make the test paper strip expose the test paper cassette like this, is enough to place the detection end and the quality control end of test paper strip in the top in detection pond and quality control pond, also is enough to cooperate the use with the absorbent paper of absorbent paper mount B bottom pasting part and absorbent paper mount A bottom pasting.
The application of the high-sensitivity detection test paper box is the application in the aspects of detecting HCG, cancers and viruses. When the to-be-detected marker exists in the sample, the solutions in the detection pool and the quality control pool are changed from red to blue; when the marker to be detected does not exist in the sample and the test paper box is effective, the color of the solution in the detection tank is not changed into red, and the solution in the quality control tank is changed from red into blue; when the test paper box fails, the color of the solution in the quality control pool is not changed into red.
The application of the high-sensitivity test paper box comprises the following specific application methods:
after a sample is dripped into the sample dripping port, the sample passes through a silver mark pad containing silver nano particles-antibodies in the test strip, and the silver nano particles-antibodies are brought to a detection end and a quality control end of the test strip along with diffusion;
the slide block is stirred, the detection end and the quality control end of the test paper strip are respectively positioned above the detection pond and the quality control pond and below the water absorbing papers adhered to the bottom parts of the two water absorbing paper fixing frames B, the pressing rod of the pressing device is pressed down, the water absorbing papers adhered to the bottom parts of the two water absorbing paper fixing frames B are respectively adhered to the detection end and the quality control end of the test paper strip, hydrogen peroxide is dripped into the sample dripping opening, the water absorbing papers at the non-adhered parts of the bottom parts of the two water absorbing paper fixing frames B become wet, the sample is led to the detection pond and the quality control pond due to the bending of the action of gravity, and then colloidal gold solution modified through terminal alkynyl is dripped into the detection pond and the quality control pond. If the sample contains the marker to be detected, the color of the solution in the detection pool is changed from red to blue; if the sample does not contain the marker to be detected, the color of the solution in the detection pool is not changed into red; if the test strip fails, the color of the solution in the quality control tank is not changed into red; if the test paper strip is effective, the color of the solution in the quality control pool is changed from red to blue.
The high-sensitivity detection paper box is applied, equipment matched with the test paper box is adopted for detection, the equipment comprises a box body, each unit arranged in the box body comprises a micro host, a bin inlet and outlet push-pull device, a camera device, a first sample adding controller and a second sample adding controller, and each unit arranged on the box body comprises a cabin door, a touch display screen, a first reagent tank, a second reagent tank and a movable tray; the micro host is used for receiving and issuing a command and displaying the equipment state and the detection result by the touch display screen; the in-out bin push-pull device is used for moving the test paper box out of the bin door or under the reagent tank II or under the camera device; the camera device is used for shooting color changes in the detection pool and the quality control pool on the test paper box, and transmitting data to the micro-host for analysis and processing; the first sample adding controller and the second sample adding controller are respectively used for controlling the dripping amount of the colloidal gold solution modified by terminal alkynyl in the first reagent tank and the second reagent tank; the movable tray fixed on the in-out warehouse pushing and pulling device is used for loading the test paper box.
The connection between the units adopts a conventional connection mode, so long as the corresponding control relation can be realized.
When the equipment is used for detecting the paper box, (1) a power switch is turned on, and the equipment is waited for finishing self-detection; (2) Opening the cabin door, driving the tray to move out of the cabin door by the cabin inlet and outlet push-pull device, putting the detection test paper box on the tray, dripping and stirring the sample at the sample dripping port, and enabling the tray to reenter the equipment; (3) When the tray reaches the corresponding position, quantitatively dripping hydrogen peroxide into the first reagent pool under the instruction of the first sample adding controller; (4) Quantitatively dripping the colloidal gold solution modified by terminal alkynyl under the instruction of a sample adding controller II in the reagent pool II; (5) The feeding and discharging push-pull device drives the test paper box on the tray to move to the position right below the camera device, and at the moment, the detection pool and the quality control pool on the test paper box are opposite to the camera device, and the camera device photographs the color changes in the detection pool and the quality control pool; (6) The camera device transmits the photo information to the micro host for analysis, and the result is uploaded to the cloud; and (7) after the detection is finished, the tray ejects the cabin door.
The test paper box is detected in a totally-enclosed environment in the device, and is not influenced by the environment such as external light, dust and the like, so that the color of the test paper box is captured more accurately by the camera device, and false positives caused by insensitivity of a user to the color are avoided; the error of manually dripping the reagent type can be avoided, the reagent can be quantitatively dripped, and the error caused by too much and too little dripping is reduced; the device is further provided with a WIFI transmitting device, data are uploaded to the database, and the mobile terminal can review detection results in real time, and is convenient and rapid.
The marker to be detected can be HCG used for early pregnancy detection, a cancer tumor marker used for cancer detection, a virus itself used for virus detection, an antigen carried by the virus or an antibody generated by a human body after the virus is infected. Namely: the detection line may be coated with a monoclonal antibody corresponding to the marker to be detected, but when detecting viruses, the detection is performed by detecting an antibody against viruses, so that when detecting viruses, the detection line may be coated with an antigen corresponding to the antibody to be detected, in addition to the antibody (antibody against an antiviral antibody). In addition, the virus itself can also be used as an antigen, and is combined with the antibody in the silver label pad and the antibody on the detection line to form a double-antibody sandwich structure. Regarding the quality control line, if a mouse antibody is used on the silver nanoparticle-antibody complex, a sheep anti-mouse antibody is used on the quality control line; if rabbit antibodies are used on the silver nanoparticle-antibody complex, goat anti-rabbit antibodies are used on the quality control line.
The high-sensitivity detection test paper box and the application thereof can detect whether the test paper strip is effective or not while detecting the sample, can accurately detect the sample with less content of the marker to be detected, have extremely high detection sensitivity, and are easy to popularize and use in medical work or home detection.
Drawings
FIG. 1 is a state diagram of the test paper cassette before use;
FIG. 2 is a view showing the usage state of the test paper box during detection and quality control;
FIG. 3 is a schematic diagram of the structure of the test strip;
FIG. 4 is a schematic diagram of the structure of the test paper holder;
FIG. 5 is a schematic diagram of the upper cover of the test paper holder;
FIG. 6 is a schematic view of the structure of the housing base;
FIG. 7 is a schematic view of the structure of the upper cover of the housing;
FIG. 8 is a schematic diagram of the pressing device when the test strip is not in use;
FIG. 9 is a schematic diagram of the pressing device after the test strip is soaked;
fig. 10 is a partial enlarged view of the hole 25;
FIG. 11 is a schematic view of the external structure of the test device mated with the cartridge;
fig. 12 is a schematic view of the internal structure of fig. 11.
In the figure, 1 is a quality control end, 2 is a detection end, 3 is a Y-shaped groove, 4 is a sliding block, 5 is a sample dropping port, 6 is a quality control pool, 7 is a detection pool, 8 is a rotating shaft, 9 is a water absorbing paper fixing frame A,10 is a groove, 11 is a hole II, 12 is a hole I, 13 is a hole III, 14 is a test paper strip, 15 is a test paper clamping seat, 16 is a test paper clamping seat upper cover, 17 is a shell base, 18 is a shell upper cover, 19 is a sample pad, 20 is a silver mark pad, 21 is a quality control line, 22 is a detection line, 23 is an NC film, 24 is a plastic rubber plate, 25 is a hole, 26 is a flexible limiting clamp, 27 is a bulge, 28 is a pressing rod, 29 is a water absorbing paper fixing frame B,30 is water absorbing paper, 31 is a box body, 32 is a micro-type host, 33 is a cabin push-pull device, 34 is a camera device, 35 is a sample adding controller I, 36 is a sample adding controller II, 37 is a cabin door, 38 is a power switch, 39 is a touch display screen, 40 is a pool I, 41 is a reagent tray II, and 42 is a movable tray.
Detailed Description
The products of the present invention are commercially available products unless specifically indicated.
1. The gold nanoparticle solution modified by terminal alkynyl is prepared
(1) Preparation of gold nanoparticles
50mL of 0.01wt% chloroauric acid is heated to a rolling boiling state in a 100mL round bottom flask, stirred vigorously and refluxed, 5mL of 38.8mmoL/L sodium citrate is added, the color of the solution is changed from pale yellow to red, heating is continued for 15 minutes, stirring is continued for 15 minutes, the obtained solution is cooled to room temperature, characterization is carried out by an ultraviolet-visible spectrophotometer, and the synthesized gold nanoparticles are stored in a centrifuge tube at 4 ℃ for standby.
(2) Synthesis of 5- (1, 2-dithiolan-3-yl) -N- (prop-2-yl) pentanamide (CAS number: 1304779-08-5)
N-thiooxysuccinimide (0.5 g,1.56 mmoL) was added dropwise to an ice water solution of propynylamine (100. Mu.L, 1.56 mmoL) in methylene chloride in 10mL of methylene chloride, and the mixture was stirred at room temperature, followed by 30mL of CH 2 Cl 2 And saturated Na 2 CO 3 The solution was diluted, and the organic layer was washed with saturated brine, and dried over anhydrous Na 2 SO 4 Drying, evaporating the solvent in vacuo, and purifying the residue by silica gel column chromatography to obtain 5- (1, 2-dithiolan-3-yl) -N- (prop-2-yl) pentanamide (CAS number: 1304779-08-5) with the following structural formula:
(3) Preparation of the gold nanoparticle solution modified by terminal alkynyl
200. Mu.L of 5- (1, 2-dithiolan-3-yl) -N- (prop-2-yl) pentanamide solution (dissolved in ethanol) was added dropwise to 10mL of the gold nanoparticle solution, the pH of the solution was adjusted to 9 with sodium hydroxide, the resulting solution was stirred at room temperature, and then centrifuged at 9000rpm for 20 minutes to remove the excess compounds, and the obtained alkynyl-functionalized gold nanoparticles were washed three times with water and then dispersed in distilled water for use.
1. Preparation of silver mark pad
(1) Preparation of silver nanoparticles
18mgAgNO 3 Dissolving in 100mL distilled water, heating to boiling, stirring vigorously, adding 2mL of 1% sodium citrate into the boiling solution, and heating for 30min; and cooling to room temperature to obtain silver nano particles, and storing in a shading bottle for standby.
(2) Binding silver nanoparticles to antibodies
Centrifugally concentrating the silver nano-particles obtained in the step (1), dispersing in 10mmoL/L (or lower) PBS (pH 7.4, avoiding chloride ions), and rapidly adding 1mL of antibody; the mixed solution was shaken with 500. Mu.L of 1% BSA for 12 hours, and blocked with 500. Mu.L of 1% BSA for 12 hours; and then centrifuging to remove excessive antibody and silver nano particles, thus obtaining the silver nano particle-antibody.
(3) Preparation of silver mark pad
Spraying the silver nano particle-antibody prepared in the step (2) on glass fiber to prepare the silver mark pad.
2. The quality control line and the detection line are respectively marked on two branches of the Y-shaped test paper by a film marking machine according to the prior art.
Examples
The high-sensitivity detection test paper box comprises a storage box for placing a test paper strip 14, the storage box comprises a test paper clamping seat 15 and a test paper clamping seat upper cover 16, the test paper clamping seat upper cover 16 is provided with a sample dropping opening 5 and a sliding block 4, one end of the test paper clamping seat 15 is rotationally connected to a shell, the shell comprises a shell base 17 and a shell upper cover 18, the shell upper cover 18 is provided with a first hole 12, a second hole 11 and a third hole 13, the sample dropping opening 5 is matched with the first hole 12, the sliding block 4 penetrates through the second hole 11 and slides in the second hole 11, and the third hole 13 is matched with a detection pool 7 and a quality control pool 6 which are arranged on the inner side of the shell base 17;
a hole 25 is arranged between the detection tank 7 and the quality control tank 6, a pressing rod 28 capable of moving downwards is arranged in the hole 25, two absorbent paper fixing frames B29 are arranged on the pressing rod 28, absorbent paper 30 with partially adhered portions are respectively arranged at the bottoms of the two absorbent paper fixing frames B29, and non-adhered portions of the two absorbent paper 30 and the absorbent paper fixing frames B29 are respectively located above the detection tank 7 and the quality control tank 6.
The hole 25 is internally provided with a flexible limiting clamp 26, the pressing rod 28 is arranged in the flexible limiting clamp 26, the flexible limiting clamp 26 is provided with a protrusion 27, the pressing rod 28 is provided with a plurality of grooves 10 which are arranged up and down and matched with the protrusion 27, and the protrusion 27 is in a state of entering any groove 10 in the downward movement process of the pressing rod 28.
The inner side of the shell base 17 is provided with a water absorbing paper fixing frame A9.
The rotary shaft 8 is arranged on the housing base 17 and is used for rotationally connecting the test paper clamping seat 15, and the test paper strip 14 in the storage box is driven to slide when the sliding block 4 is shifted.
The test strip 14 comprises a sample pad 19, a silver mark pad 20, an NC film 23 and a plastic rubber plate 24, wherein the test strip 14 is Y-shaped, and the tail end of the test strip 14 is provided with a detection end 2 with a detection line and a quality control end 1 with a quality control line.
The application of the high-sensitivity detection test paper box is the application in the aspects of detecting HCG, cancers and viruses.
The application of the high-sensitivity test paper box comprises the following specific application methods:
after the sample is dripped into the sample dripping port 5, the sample passes through a silver mark pad 20 containing silver nano particles-antibodies in the test strip 14, and the silver nano particles-antibodies are brought to a detection end 2 and a quality control end 1 of the test strip 14 along with diffusion;
the slide block 4 is shifted to enable the detection end 2 and the quality control end 1 of the test paper strip 14 to be respectively located above the detection pond 7 and the quality control pond 6 and below the water absorption paper 30 stuck on the bottom parts of the two water absorption paper fixing frames B29, the pressing rod 28 of the pressing device is pressed down to enable the water absorption paper 30 stuck on the bottom parts of the two water absorption paper fixing frames B29 to be respectively attached to the detection end 2 and the quality control end 1 of the test paper strip 14, hydrogen peroxide is dripped into the sample dripping opening 5, the water absorption paper 30 not stuck on the bottom parts of the two water absorption paper fixing frames B29 is wetted, and is bent under the action of gravity to respectively enter the detection pond 7 and the quality control pond 6, so that liquid is drained to the detection pond 7 and the quality control pond 6, and then colloidal gold solution modified through terminal alkynyl is dripped into the detection pond 7 and the quality control pond 6. If the sample contains the marker to be detected, the color of the solution in the detection cell 7 is changed from red to blue; if the sample does not contain the marker to be detected, the color of the solution in the detection tank 7 is not changed into red; if the test strip fails, the color of the solution in the quality control tank 6 is not changed into red; if the test strip is effective, the color of the solution in the quality control pool 6 is changed from red to blue.
The application of the high-sensitivity test paper box adopts equipment matched with the test paper box for detection, the equipment comprises a box body 31, each unit arranged in the box body 31 comprises a micro host 32, a bin inlet and outlet push-pull device 33, a camera device 34, a first sample adding controller 35 and a second sample adding controller 36, and each unit arranged on the box body 31 comprises a cabin door 37, a touch display screen 39, a first reagent tank 40, a second reagent tank 41 and a movable tray 42; the micro-host 32 is used for receiving and issuing a command, and displaying the device state and the detection result by the touch display screen (39); the warehouse-in and warehouse-out push-pull device 33 is used for moving the test paper box out of the cabin door 37 or under the first reagent tank 40 or under the second reagent tank 41 or under the camera device 34; the camera device 34 is used for shooting color changes in the detection pool 7 and the quality control pool 6 on the test paper box, and transmitting data to the micro-host 32 for analysis and processing; the first sample adding controller 35 and the second sample adding controller 36 are respectively used for controlling the dripping amount of the colloidal gold solution modified by terminal alkynyl in the first reagent tank 40 and the second reagent tank 41; the movable tray 42 fixed to the in-and-out magazine 33 is used to load the test paper cassettes.
When the equipment inspection test paper box is used, (1) the power switch 38 is turned on, and the equipment self-inspection is waited for; (2) Opening the cabin door 37, driving the movable tray 42 to move out of the cabin door 37 by the cabin inlet and outlet pushing and pulling device 33, placing the detection test paper box on the movable tray 42, and enabling the movable tray 42 to reenter the equipment after the sample is dripped and stirred at the sample dripping port 5; (3) When the movable tray 42 reaches the corresponding position, the first reagent tank 40 quantitatively drops hydrogen peroxide under the instruction of the first sample adding controller 35; (4) The reagent tank II 41 quantitatively drops the colloidal gold solution modified by terminal alkynyl under the instruction of the sample adding controller II 36; (5) The in-out bin push-pull device 33 drives the test paper box on the movable tray 42 to move to the position right below the image pickup device 34, at the moment, the detection pool 7 and the quality control pool 6 on the kit are opposite to the image pickup device 34, and the image pickup device 34 photographs the color changes in the detection pool 7 and the quality control pool 6; (6) The camera device 34 transmits the photo information to the micro-host 32 for analysis, and the result is uploaded to the cloud; (7) after the detection, the movable tray 42 ejects the hatch 37.
Test examples
The high-sensitivity detection test paper box is used for detecting HCG, cancers and viruses, samples containing the three markers to be detected are respectively taken, and the detection results are as follows:
Claims (4)
1. the utility model provides a high sensitivity detects test paper box, includes the storage box of placing test paper (14), the storage box comprises test paper cassette (15) and test paper cassette upper cover (16), its characterized in that: the test paper cassette upper cover (16) is provided with a sample dropping opening (5) and a sliding block (4), one end of the test paper cassette (15) is rotationally connected to the shell, the shell consists of a shell base (17) and a shell upper cover (18), the shell upper cover (18) is provided with a first hole (12), a second hole (11) and a third hole (13), the sample dropping opening (5) is matched with the first hole (12), the sliding block (4) penetrates through the second hole (11) and slides in the second hole (11), and the third hole (13) is matched with a detection pool (7) and a quality control pool (6) which are arranged on the inner side of the shell base (17);
a hole (25) is formed between the detection pool (7) and the quality control pool (6), a pressing rod (28) capable of moving downwards is arranged in the hole (25), two water-absorbing paper fixing frames B (29) are arranged on the pressing rod (28), water-absorbing papers (30) which are partially pasted are respectively arranged at the bottoms of the two water-absorbing paper fixing frames B (29), and non-pasted parts of the two water-absorbing papers (30) and the water-absorbing paper fixing frames B (29) are respectively positioned above the detection pool (7) and the quality control pool (6);
a water absorbing paper fixing frame A (9) is arranged on the inner side of the shell base (17);
the test strip (14) comprises a sample pad (19), a silver mark pad (20), an NC film (23) and a plastic rubber plate (24), wherein the test strip (14) is Y-shaped, and the tail end of the test strip (14) is provided with a detection end (2) with a detection line and a quality control end (1) with a quality control line;
the specific application method of the high-sensitivity test paper box comprises the following steps:
after the sample is dripped into the sample dripping port (5), the sample passes through a silver label pad (20) containing silver nano particles-antibodies in the test strip (14), and the silver nano particles-antibodies are brought to a detection end (2) and a quality control end (1) of the test strip (14) along with the diffusion action;
the slide block (4) is stirred, so that the detection end (2) and the quality control end (1) of the test strip (14) are respectively positioned above the detection tank (7) and the quality control tank (6), the lower parts of the water absorption papers (30) adhered to the bottom parts of the two water absorption paper fixing frames B (29) are respectively adhered to the detection end (2) and the quality control end (1) of the test strip (14) by pressing a pressing rod (28) of a pressing device, hydrogen peroxide is dripped into a sample dripping port (5), the water absorption papers (30) at the non-adhered parts at the bottoms of the two water absorption paper fixing frames B (29) are wetted, the water absorption papers respectively enter the detection tank (7) and the quality control tank (6) due to bending of gravity, liquid is drained to the detection tank (7) and the quality control tank (6), and then a colloidal gold solution subjected to terminal alkynyl modification is dripped into the detection tank (7) and the quality control tank (6); if the sample contains the marker to be detected, the color of the solution in the detection pool (7) is changed from red to blue; if the sample does not contain the marker to be detected, the color of the solution in the detection tank (7) is not changed into red; if the test strip fails, the color of the solution in the quality control tank (6) is not changed into red; if the test paper strip is effective, the color of the solution in the quality control pool (6) is changed from red to blue.
2. The high sensitivity test carton of claim 1, wherein: be provided with flexible spacing clamp (26) in hole (25), press pole (28) to set up in flexible spacing clamp (26), be equipped with protruding (27) on flexible spacing clamp (26), press to set up on pole (28) a plurality of upper and lower ranges and with protruding (27) assorted recess (10), in pressing pole (28) at the in-process of moving down, protruding (27) have the state of getting into arbitrary recess (10).
3. A high sensitivity test paper cassette according to any one of claims 1-2, wherein: the high-sensitivity detection test paper box is used for detecting HCG, cancers and viruses.
4. A high sensitivity test paper cassette according to any one of claims 1-2, wherein: the detection is carried out by adopting equipment matched with the test paper box, the equipment comprises a box body (31), each unit arranged in the box body (31) comprises a miniature host machine (32), a warehouse-in and warehouse-out push-pull device (33), a camera device (34), a first sample adding controller (35) and a second sample adding controller (36), and each unit arranged on the box body (31) comprises a cabin door (37), a touch display screen (39), a first reagent tank (40), a second reagent tank (41) and a movable tray (42); the micro host (32) is used for receiving and issuing a command, and displaying the equipment state and the detection result by the touch display screen (39); the in-out bin pushing and pulling device (33) is used for moving the test paper box out of the cabin door (37) or under the first reagent tank (40) or under the second reagent tank (41) or under the camera device (34); the camera device (34) is used for shooting color changes in the detection pool (7) and the quality control pool (6) on the test paper box, and transmitting data to the micro-host (32) for analysis and processing; the first sample adding controller (35) and the second sample adding controller (36) are respectively used for controlling the dripping amount of the colloidal gold solution modified by terminal alkynyl in the first hydrogen peroxide solution in the reagent tank (40) and the second hydrogen peroxide solution in the reagent tank (41); a movable tray (42) fixed to the in-and-out hopper push-pull device (33) is used for loading the test paper cassettes.
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