CN113189327A - High-sensitivity detection test paper box and application thereof - Google Patents
High-sensitivity detection test paper box and application thereof Download PDFInfo
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- CN113189327A CN113189327A CN202110506363.2A CN202110506363A CN113189327A CN 113189327 A CN113189327 A CN 113189327A CN 202110506363 A CN202110506363 A CN 202110506363A CN 113189327 A CN113189327 A CN 113189327A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
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Abstract
The invention discloses a high-sensitivity detection test paper box and application thereof.A test paper strip adopts a Y-shaped structure formed by a detection end with a detection line and a quality control end with a quality control line, and a detection test paper box which is matched with the detection test paper box and comprises a quality control pool and a detection pool is used.
Description
Technical Field
The invention belongs to the technical field of medical treatment, and particularly relates to a high-sensitivity detection test paper box and application thereof.
Background
At present, the detection test paper box on the market adopts a double-antibody sandwich one-step method which takes colloidal gold coupled with an antibody as an indication mark to detect the marker to be detected in a sample. When the detection test paper is used for detection, when a sample to be detected passes through the colloidal gold coupled antibody, a marker to be detected-antibody colloidal gold compound is formed, the compound is continuously diffused, when the compound reaches a detection line (coated with a monoclonal antibody corresponding to the marker to be detected), a double-antibody sandwich colloidal gold compound is formed, a color band is shown at the detection line, excessive colloidal gold antibody continuously crawls, and a quality control line antibody (used for detecting whether the antibody is inactivated) forms a colloidal gold immune compound, and the color band is shown. However, due to the limitation of detection sensitivity, only when the content of the marker to be detected in the sample reaches a certain level, a relatively accurate detection result can be obtained. If the sample contains less markers to be detected, an accurate detection result cannot be obtained.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a high-sensitivity detection test paper box and application thereof, solves the technical problem that the detection result is inaccurate or can not be detected when a sample contains a small amount of markers, and reduces the detection limit.
The technical scheme of the invention is realized as follows:
a high-sensitivity detection test paper box comprises a storage box for placing test paper strips, wherein the storage box consists of a test paper clamping seat and a test paper clamping seat upper cover, the test paper clamping seat upper cover is provided with a sample dropping hole and a sliding block, one end of the test paper clamping seat is rotatably connected to a shell, the shell consists of a shell base and a shell upper cover, the shell upper cover is provided with a hole I, a hole II and a hole III, the sample dropping hole is matched with the hole I for use, the sliding block penetrates through the hole II and slides in the hole II for use, and the hole III is matched with a detection pool and a quality control pool which are arranged on the inner side of the shell base for use;
the detection device is characterized in that a hole is formed between the detection pool and the quality control pool, two absorbent paper fixing frames B are arranged on a pressing rod of a pressing rod which can move downwards in the hole, absorbent paper which is partially adhered is arranged at the bottoms of the two absorbent paper fixing frames B respectively, and the parts of the two absorbent paper which are not adhered to the absorbent paper fixing frames B are located above the detection pool and the quality control pool respectively.
The flexible limiting clamp is arranged in the hole, the pressing rod is arranged in the flexible limiting clamp, a protrusion is arranged on the flexible limiting clamp, a plurality of grooves which are arranged up and down and matched with the protrusion are arranged on the pressing rod, and the protrusion has a state of entering any groove in the downward moving process of the pressing rod.
The pressing rod and the two absorbent paper fixing frames B form a pressing device. When the sliding block is shifted, the sliding block drives the test strip in the storage box to move to the positions above the detection pool and the quality control pool and below the two absorbent paper fixing frames B of the pressing device, at the moment, the parts, which are not adhered to the two absorbent paper fixing frames B, are respectively positioned above the detection pool and the quality control pool, and the test strip has a certain distance from the two absorbent paper fixing frames B above and the detection pool and the quality control pool below, so that the test strip is prevented from being damaged due to friction when the sliding block is shifted, and in addition, the tail end of the test strip is pressed to be closer to the absorbent paper; in addition, two absorbent paper mount B bottoms are provided with the absorbent paper that part was pasted respectively, promptly: the absorbent paper is arranged at the bottom of the absorbent paper fixing frame B, one part of the absorbent paper is adhered to the bottom of the absorbent paper fixing frame B, the other part of the absorbent paper is not adhered, so that the absorbent paper of the non-adhered part and the absorbent paper of the adhered part are on the same plane under the dry condition, and the absorbent paper of the non-adhered part is naturally bent under the action of gravity under the wet condition of the absorbent paper.
The storage box for placing the test strip is composed of a test strip clamping seat and a test strip clamping seat upper cover, and preferably, the test strip clamping seat and the test strip clamping seat upper cover are provided with buckle dismounting structures; the shell consists of a shell base and a shell upper cover, and preferably, the shell base and the shell upper cover are in a snap-in and disassembly structure.
The inner side of the shell base is provided with a water absorption paper fixing frame A, and the bottom of the shell base is adhered with water absorption paper which is used for absorbing the silver nanoparticle-antibody which is not combined with the quality control line/detection line after the sample is dripped, and simultaneously promoting the forward flowing of the sample and the marker-antibody-silver nanoparticle compound to be detected.
The test paper box is characterized in that a rotating shaft is arranged on the base of the shell and used for rotating and connecting the test paper clamping seat, and the test paper clamping seat drives the test paper in the storage box to slide when the sliding block is stirred.
The test strip comprises a sample pad, a silver label pad, an NC membrane and a plastic rubber plate, the test strip is Y-shaped, and the tail end of the test strip is provided with a detection end with a detection line and a quality control end with a quality control line. The detection end with the detection line is used for detecting whether a sample contains a marker to be detected, and the quality control end with the quality control line is used for detecting whether the test strip contains silver nanoparticle-antibody or whether the silver nanoparticle-antibody fails. Therefore, whether the test strip is invalid or not can be detected while the marker to be detected is detected, detection and quality control are avoided being carried out respectively, and detection time is saved. When the test strip is used, the test strip slides to the detection pool and the quality control pool, and the detection end and the quality control end of the test strip are respectively positioned above the detection pool and the quality control pool.
The test strip is Y-shaped and is placed in a Y-shaped groove formed in the test strip clamping seat, the length of the Y-shaped test strip is larger than that of the Y-shaped groove, so that the test strip can be exposed out of the test strip clamping seat, the detection end and the quality control end of the test strip can be sufficiently arranged above the detection pool and the quality control pool, and the detection end and the quality control end of the test strip can be sufficiently matched with the absorbent paper adhered to the bottom of the absorbent paper fixing frame B and the absorbent paper adhered to the bottom of the absorbent paper fixing frame A for use.
The application of the high-sensitivity detection test paper box is the application in the aspect of detecting HCG, cancer and virus. When the marker to be detected exists in the sample, the solution in the detection pool and the quality control pool is changed from red to blue; when the marker to be detected does not exist in the sample and the test paper box is effective, the color of the solution in the detection pool is not changed into red, and the color of the solution in the quality control pool is changed from red into blue; when the test paper box fails, the color of the solution in the quality control pool does not change into red.
The application of the high-sensitivity detection test paper box comprises the following specific application methods:
after a sample is dripped into the sample dripping port, the sample passes through a silver label pad containing silver nanoparticles and antibodies in the test strip, and the silver nanoparticles and antibodies are brought to the detection end and the quality control end of the test strip along with the diffusion effect;
the slide block is shifted, so that the detection end and the quality control end of the test strip are respectively positioned above the detection pool and the quality control pool, the lower parts of the absorbent papers pasted on the bottom parts of the two absorbent paper fixing frames B are pressed down, the pressing rod of the pressing device is pressed down, the absorbent papers pasted on the bottom parts of the two absorbent paper fixing frames B are respectively pasted with the detection end and the quality control end of the test strip, hydrogen peroxide is dripped at a sample dripping port, the absorbent papers of the parts which are not pasted on the bottom parts of the two absorbent paper fixing frames B become wet, the absorbent papers are bent under the action of gravity and respectively enter the detection pool and the quality control pool, a sample is drained to the detection pool and the quality control pool, and then colloidal gold solution decorated by terminal alkyne is dripped in the detection pool and the. If the sample contains the marker to be detected, the color of the solution in the detection pool is changed from red to blue; if the sample does not contain the marker to be detected, the color of the solution in the detection pool is unchanged to red; if the test strip is invalid, the color of the solution in the quality control pool is unchanged to red; if the test strip is effective, the color of the solution in the quality control pool is changed from red to blue.
The high-sensitivity detection test paper box is applied to detection by adopting equipment matched with the test paper box, the equipment comprises a box body, each unit arranged in the box body comprises a micro host, an in-and-out bin push-pull device, a camera device, a first sample adding controller and a second sample adding controller, and each unit arranged on the box body comprises a cabin door, a touch display screen, a first reagent pool, a second reagent pool and a movable tray; the micro host is used for receiving and issuing commands and displaying the equipment state and the detection result by the touch display screen; the in-out bin push-pull device is used for moving the test paper box out of the bin door or below the first reagent pool or the second reagent pool or below the camera device; the camera device is used for shooting color changes in the detection pool and the quality control pool on the test paper box and transmitting data to the micro host for analysis and processing; the first sample adding controller and the second sample adding controller are respectively used for controlling the dropping amount of hydrogen peroxide in the first reagent pool and the colloidal gold solution modified by terminal alkynyl in the second reagent pool; the movable tray fixed on the in-out bin push-pull device is used for loading the test paper box.
The connection between the units adopts a conventional connection mode as long as the corresponding control relation can be realized.
When the test paper box is detected by using the equipment, (1) a power switch is turned on, and the equipment is waited to be self-detected; (2) opening the cabin door, driving the tray to move out of the cabin door by the in-out cabin push-pull device, placing the detection test paper box on the tray, dripping a sample at the sample dripping port and stirring, and then enabling the tray to enter the equipment again; (3) when the tray reaches the corresponding position, the first reagent pool is quantitatively dripped with hydrogen peroxide under the instruction of the first sample adding controller; (4) quantitatively dripping a colloidal gold solution modified by terminal alkynylation into the reagent pool II under the instruction of the sample adding controller II; (5) the in-out bin push-pull device drives the test paper box on the tray to move to the position right below the camera device, at the moment, the detection pool and the quality control pool on the test paper box are right opposite to the camera device, and the camera device shoots the color change in the detection pool and the quality control pool; (6) the camera device transmits the photo information to the micro host for analysis, and uploads the result to the cloud; (7) and after the detection is finished, ejecting the tray out of the cabin door.
The test paper box is detected in a totally-enclosed environment in the equipment, and is not influenced by external light, dust and other environments, so that the color of the test paper box is captured more accurately by the camera device, and false positive caused by insensitivity of a user to the color is avoided; the error in the type of the reagent which is artificially dripped can be avoided, and meanwhile, the reagent can be quantitatively dripped, so that the error caused by too much and too little dripping is reduced; the device also comprises a WIFI transmitting device, and the data is uploaded to the database, so that the mobile terminal can look up the detection result in real time, and the device is convenient and quick.
The marker to be detected can be HCG for early pregnancy detection, a cancer tumor marker for cancer detection, virus for virus detection, an antigen carried by the virus or an antibody generated by a human body after the virus is infected. Namely: the detection line may be coated with a monoclonal antibody corresponding to the marker to be detected, but since the detection is carried out by substantially detecting an anti-viral antibody when detecting a virus, the detection line may be coated with an antigen corresponding to the antibody to be detected, in addition to the antibody (anti-viral antibody) when detecting a virus. In addition, the virus itself can also be used as an antigen, and is combined with the antibody in the silver label pad and the antibody on the detection line to form a double-antibody sandwich structure. Regarding the quality control line, if a mouse antibody is used on the silver nanoparticle-antibody complex, a goat anti-mouse antibody is used on the quality control line; if rabbit antibody is used on the silver nanoparticle-antibody complex, goat anti-rabbit antibody is used on the quality control line.
The high-sensitivity detection test paper box and the application thereof can detect whether the test paper is effective or not while detecting a sample, can accurately detect under the condition that the content of a marker to be detected in the sample is less, have extremely high detection sensitivity, and are easy to popularize and use in medical work or home detection.
Drawings
FIG. 1 is a state diagram of the test paper cassette before use;
FIG. 2 is a diagram showing the state of use of the test paper cassette in the detection and quality control;
FIG. 3 is a schematic structural diagram of the test strip;
FIG. 4 is a schematic structural diagram of the test paper holder;
FIG. 5 is a schematic structural diagram of the test paper cassette upper cover;
FIG. 6 is a schematic structural view of the housing base;
FIG. 7 is a schematic structural view of the upper cover of the housing;
FIG. 8 is a schematic view of the pressing device when the test strip is not in use;
FIG. 9 is a schematic view of the pressing means after the test strip is wetted;
FIG. 10 is an enlarged view of a portion of the hole 25;
fig. 11 is an external structure view of a detecting device matched with the test paper box;
fig. 12 is a schematic view of the internal structure of fig. 11.
In the figure, 1 is a quality control end, 2 is a detection end, 3 is a Y-shaped groove, 4 is a sliding block, 5 is a sample dropping port, 6 is a quality control pool, 7 is a detection pool, 8 is a rotating shaft, 9 is a water-absorbing paper fixing frame A, 10 is a groove, 11 is a second hole, 12 is a first hole, 13 is a third hole, 14 is a test strip, 15 is a test strip clamping seat, 16 is a test strip clamping seat upper cover, 17 is a shell base, 18 is a shell upper cover, 19 is a sample pad, 20 is a silver mark pad, 21 is a quality control line, 22 is a detection line, 23 is an NC film, 24 is a plastic rubber plate, 25 is a hole, 26 is a flexible limiting clamp, 27 is a bulge, 28 is a pressing rod, 29 is a water-absorbing paper fixing frame B, 30 is water-absorbing paper, 31 is a box body, 32 is a micro host, 33 is an in-out and out bin push-in device, 34 is a camera device, 35 is a first sample adding controller, 36 is a second sample adding controller, 37 is a bin door, 38 is a power switch, 39 is a touch display screen, 40 is a reagent well one, 41 is a reagent well two, and 42 is a movable tray.
Detailed Description
The products of the invention are all commercially available products, unless otherwise specified.
1. Preparing the gold nanoparticle solution with the alkynyl modified tail end
(1) Preparation of gold nanoparticles
In a 100mL round bottom flask, 50mL of 0.01 wt% chloroauric acid is heated to boiling, stirred vigorously and refluxed, 5mL of 38.8mmoL/L sodium citrate is added, the solution changes from light yellow to red in color, heating is continued for 15 minutes, stirring is continued for 15 minutes, the obtained solution is cooled to room temperature, an ultraviolet-visible spectrophotometer is used for characterization, and the synthesized gold nanoparticles are stored in a centrifuge tube at 4 ℃ for standby.
(2) Synthesis of 5- (1,2-dithiolan-3-yl) -N- (prop-2-ynyl) pentamide (CAS No.: 1304779-08-5)
To an ice-water solution of propynylamine (100. mu.L, 1.56mmoL) in methylene chloride was added dropwise N-sulfoxy succinimide (0.5g, 1.56mmoL) in 10mL of methylene chloride, and the mixture was stirred at room temperature and then with 30mL of CH2Cl2And saturated Na2CO3The solution was diluted, and the organic layer was washed with saturated brine and anhydrous Na2SO4Drying, evaporating the solvent in vacuo, and purifying the residue by silica gel column chromatography to obtain 5- (1,2-dithiolan-3-yl) -N- (prop-2-ynyl) pentanamide (CAS No.: 1304779-08-5) having the following structural formula:
(3) preparation of the gold nanoparticle solution modified by terminal alkynylation
200 μ L of 5- (1,2-dithiolan-3-yl) -N- (prop-2-ynyl) pentanamide solution (dissolved in ethanol) was added dropwise to 10mL of the gold nanoparticle solution, the pH of the solution was adjusted to 9 with sodium hydroxide, the resulting solution was stirred at room temperature, and then centrifuged at 9000rpm for 20 minutes to remove excess compound, and the resulting alkynyl-functionalized gold nanoparticles were washed three times with water and redispersed in distilled water for further use.
1. Preparation of silver label pad
(1) Preparation of silver nanoparticles
18mgAgNO3Dissolving in 100mL of distilled water, heating to boil, stirring vigorously, adding 2mL of 1% sodium citrate into the boiling solution, and continuing to heat for 30 min; and cooling to room temperature to obtain silver nanoparticles, and storing in a shading bottle for later use.
(2) Binding silver nanoparticles to antibodies
Centrifugally concentrating the silver nanoparticles obtained in the step (1), dispersing the silver nanoparticles in 10mmoL/L (or lower) of PBS (pH 7.4 to avoid chloride ions), and quickly adding 1mL of antibody; shaking the mixed solution with 500 μ L1% BSA for 12h, and blocking with 500 μ L1% BSA for 12 h; and then centrifuging to remove the redundant antibody and silver nanoparticles to obtain silver nanoparticle-antibody.
(3) Preparation of silver label pad
And (3) spraying the silver nanoparticle-antibody prepared in the step (2) on glass fiber to prepare a silver label pad.
2. And the quality control line and the detection line are respectively scribed on two branches of the Y-shaped test paper by adopting a scribing machine according to the prior art.
Examples
The high-sensitivity detection test paper box comprises a storage box for placing test paper strips 14, wherein the storage box consists of a test paper clamping seat 15 and a test paper clamping seat upper cover 16, the test paper clamping seat upper cover 16 is provided with a sample dropping port 5 and a sliding block 4, one end of the test paper clamping seat 15 is rotatably connected to a shell, the shell consists of a shell base 17 and a shell upper cover 18, the shell upper cover 18 is provided with a hole I12, a hole II 11 and a hole III 13, the sample dropping port 5 is matched with the hole I12 for use, the sliding block 4 is matched with the hole II 11 and slides in the hole II 11 for use, and the hole III 13 is matched with a detection pool 7 and a quality control pool 6 which are arranged on the inner side of the shell base 17 for use;
a hole 25 is formed between the detection pool 7 and the quality control pool 6, a pressing rod 28 capable of moving downwards is arranged in the hole 25, two absorbent paper fixing frames B29 are arranged on the pressing rod 28, absorbent paper 30 which is partially adhered is arranged at the bottoms of the two absorbent paper fixing frames B29 respectively, and the parts, which are not adhered, of the two absorbent paper 30 and the absorbent paper fixing frame B29 are respectively positioned above the detection pool 7 and the quality control pool 6.
The hole 25 is internally provided with a flexible limiting clamp 26, the pressing rod 28 is arranged in the flexible limiting clamp 26, the flexible limiting clamp 26 is provided with a bulge 27, the pressing rod 28 is provided with a plurality of grooves 10 which are vertically arranged and matched with the bulge 27, and the bulge 27 enters any one of the grooves 10 in the downward moving process of the pressing rod 28.
And a water-absorbing paper fixing frame A9 is arranged on the inner side of the shell base 17.
The shell base 17 is provided with a rotating shaft 8 for rotatably connecting with a test paper clamping seat 15, and the test paper 14 in the storage box is driven to slide when the sliding block 4 is pulled.
The test strip 14 comprises a sample pad 19, a silver label pad 20, an NC membrane 23 and a plastic rubber plate 24, the test strip 14 is Y-shaped, and the tail end of the test strip 14 is provided with a detection end 2 with a detection line and a quality control end 1 with a quality control line.
The application of the high-sensitivity detection test paper box is the application in the aspect of detecting HCG, cancer and virus.
The application of the high-sensitivity detection test paper box comprises the following specific application methods:
after the sample is dripped into the sample dripping port 5, the sample passes through a silver label pad 20 containing silver nano particles-antibodies in the test strip 14, and the silver nano particles-antibodies are brought to the detection end 2 and the quality control end 1 of the test strip 14 along with the diffusion effect;
the slide block 4 is shifted to enable the detection end 2 and the quality control end 1 of the test strip 14 to be respectively positioned above the detection pool 7 and the quality control pool 6 and below the absorbent paper 30 adhered to the bottom parts of the two absorbent paper fixing frames B29, the pressing rod 28 of the pressing device is pressed to enable the absorbent paper 30 adhered to the bottom parts of the two absorbent paper fixing frames B29 to be respectively adhered to the detection end 2 and the quality control end 1 of the test strip 14, hydrogen peroxide is dripped into the sample dripping port 5, the absorbent paper 30 of the parts which are not adhered to the bottom parts of the two absorbent paper fixing frames B29 becomes wet, the absorbent paper bends under the action of gravity and respectively enters the detection pool 7 and the quality control pool 6, liquid is drained to the detection pool 7 and the quality control pool 6, and then colloidal gold solution which is subjected to alkyne-based modification at the tail end is dripped into the detection pool 7 and the quality control pool 6. If the sample contains the marker to be detected, the color of the solution in the detection pool 7 is changed from red to blue; if the sample does not contain the marker to be detected, the color of the solution in the detection pool 7 does not change into red; if the test strip is invalid, the color of the solution in the quality control pool 6 is unchanged to red; if the test strip is effective, the color of the solution in the quality control pool 6 is changed from red to blue.
The high-sensitivity detection test paper box is applied to detection by adopting equipment matched with the test paper box, the equipment comprises a box body 31, each unit arranged in the box body 31 comprises a micro host 32, an in-and-out bin push-pull device 33, a camera device 34, a first sample adding controller 35 and a second sample adding controller 36, and each unit arranged on the box body 31 comprises a cabin door 37, a touch display screen 39, a first reagent pool 40, a second reagent pool 41 and a movable tray 42; the micro host 32 is used for receiving and issuing commands, and displaying the equipment state and the detection result by a touch display screen (39); the in-out bin pushing and pulling device 33 is used for moving the test paper box out of the compartment door 37 or below the first reagent pool 40 or the second reagent pool 41 or below the camera device 34; the camera device 34 is used for shooting color changes in the detection pool 7 and the quality control pool 6 on the test paper box, and transmitting data to the micro host 32 for analysis and processing; the first sample adding controller 35 and the second sample adding controller 36 are respectively used for controlling the dropping amount of hydrogen peroxide in the reagent pool I40 and the colloidal gold solution modified by terminal alkynylation in the reagent pool II 41; the movable tray 42 fixed to the in-out push-pull 33 is used to load the test paper cassettes.
When the test paper box is detected by the equipment, (1) the power switch 38 is turned on, and the equipment self-detection is waited to be completed; (2) opening the hatch door 37, driving the movable tray 42 to move out of the hatch door 37 by the in-out bin push-pull device 33, placing the detection test paper box on the movable tray 42, and after dropping and stirring the sample at the sample dropping port 5, enabling the movable tray 42 to enter the device again; (3) when the movable tray 42 reaches the corresponding position, the reagent pool I40 is quantitatively dripped with hydrogen peroxide under the instruction of the sample adding controller I35; (4) quantitatively dripping a colloidal gold solution modified by terminal ethynylation in the reagent pool II 41 under the instruction of the sample adding controller II 36; (5) the in-out bin push-pull device 33 drives the test paper box on the movable tray 42 to move to the position right below the camera device 34, at the moment, the detection pool 7 and the quality control pool 6 on the test paper box are opposite to the camera device 34, and the camera device 34 takes pictures of color changes in the detection pool 7 and the quality control pool 6; (6) the camera device 34 transmits the photo information to the micro host 32 for analysis, and uploads the result to the cloud; (7) after the detection is finished, the movable tray 42 ejects the hatch 37.
Test examples
The high-sensitivity detection test paper box is used for detecting HCG, cancer and virus, samples containing the three markers to be detected are respectively taken, and the detection results are as follows:
Claims (7)
1. the utility model provides a high sensitivity detects test paper box, is including placing the storage box of test paper strip (14), the storage box comprises test paper cassette (15) and test paper cassette upper cover (16), its characterized in that: the test paper clamping seat upper cover (16) is provided with a sample dropping port (5) and a sliding block (4), one end of the test paper clamping seat (15) is rotatably connected to the shell, the shell consists of a shell base (17) and a shell upper cover (18), the shell upper cover (18) is provided with a first hole (12), a second hole (11) and a third hole (13), the sample dropping port (5) is matched with the first hole (12) for use, the sliding block (4) penetrates through the second hole (11) and slides in the second hole (11) for use, and the third hole (13) is matched with a detection pool (7) and a quality control pool (6) which are arranged on the inner side of the shell base (17) for use;
be provided with hole (25) between detection pond (7) and quality control pond (6), be provided with press rod (28) that can move down in hole (25), be provided with two absorbent paper mount B (29) on press rod (28), two absorbent paper mount B (29) bottoms are provided with absorbent paper (30) that the part was pasted respectively, and two absorbent paper (30) are located the top in detection pond (7) and quality control pond (6) respectively with the part that absorbent paper mount B (29) did not paste.
2. The high-sensitivity detection test paper box according to claim 1, characterized in that: the flexible limiting clamp is characterized in that a flexible limiting clamp (26) is arranged in the hole (25), the pressing rod (28) is arranged in the flexible limiting clamp (26), a protrusion (27) is arranged on the flexible limiting clamp (26), a plurality of grooves (10) which are arranged up and down and matched with the protrusion (27) are arranged on the pressing rod (28), and the protrusion (27) has a state of entering any groove (10) in the downward moving process of the pressing rod (28).
3. The high-sensitivity detection test paper box according to claim 1, characterized in that: and a water absorption paper fixing frame A (9) is arranged on the inner side of the shell base (17).
4. The high-sensitivity detection test paper box according to claim 1, characterized in that: the test strip (14) comprises a sample pad (19), a silver label pad (20), an NC membrane (23) and a plastic rubber plate (24), the test strip (14) is Y-shaped, and the tail end of the test strip (14) is provided with a detection end (2) with a detection line and a quality control end (1) with a quality control line.
5. The use of a high sensitivity test paper cassette according to any of claims 1-4, wherein: the application in detecting HCG, cancer and virus.
6. The use of the high sensitivity detection test paper box according to claim 5, characterized in that: the specific application method is as follows:
after a sample is dripped into the sample dripping port (5), the sample passes through a silver label pad (20) containing silver nanoparticles and antibodies in the test strip (14), and the silver nanoparticles and antibodies are brought to the detection end (2) and the quality control end (1) of the test strip (14) along with diffusion;
the slide block (4) is shifted to ensure that the detection end (2) and the quality control end (1) of the test strip (14) are respectively positioned above the detection pool (7) and the quality control pool (6) and below the absorbent paper (30) adhered to the bottom parts of the two absorbent paper fixing frames B (29), the pressing rod (28) of the pressing device is pressed down, so that the absorbent paper (30) adhered to the bottom parts of the two absorbent paper fixing frames B (29) are respectively adhered to the detection end (2) and the quality control end (1) of the test strip (14), hydrogen peroxide is dripped into the sample dripping port (5), the water absorbing paper (30) on the part which is not adhered at the bottom of the two water absorbing paper fixing frames B (29) becomes wet, the liquid is respectively led into the detection pool (7) and the quality control pool (6) due to the bending of the gravity, and is drained to the detection pool (7) and the quality control pool (6), then, colloidal gold solution modified by terminal alkynylation is dripped into the detection pool (7) and the quality control pool (6). If the sample contains the marker to be detected, the color of the solution in the detection pool (7) is changed from red to blue; if the sample does not contain the marker to be detected, the color of the solution in the detection pool (7) is not changed into red; if the test strip is invalid, the color of the solution in the quality control pool (6) is unchanged to red; if the test strip is effective, the color of the solution in the quality control pool (6) is changed from red to blue.
7. The use of the high sensitivity detection test paper box according to claim 5, characterized in that: the device matched with the test paper box is adopted for detection, the device comprises a box body (31), each unit arranged in the box body (31) comprises a micro host (32), a warehouse entering and exiting push-pull device (33), a camera device (34), a sample adding controller I (35) and a sample adding controller II (36), and each unit arranged on the box body (31) comprises a cabin door (37), a touch display screen (39), a reagent pool I (40), a reagent pool II (41) and a movable tray (42); the micro host (32) is used for receiving and issuing commands and displaying the equipment state and the detection result by the touch display screen (39); the in-out bin pushing and pulling device (33) is used for moving the test paper box out of the compartment door (37) or under the reagent pool I (40) or under the reagent pool II (41) or under the camera device (34); the camera device (34) is used for shooting color changes in the detection pool (7) and the quality control pool (6) on the test paper box, and transmitting data to the micro host (32) for analysis and processing; the first sample adding controller (35) and the second sample adding controller (36) are respectively used for controlling the dropping amount of hydrogen peroxide in the reagent pool I (40) and the colloidal gold solution modified by terminal alkynyl in the reagent pool II (41); the movable tray (42) fixed on the in-out bin push-pull device (33) is used for loading test paper boxes.
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