CN113166757A - 核酸文库的制备和分析方法 - Google Patents

核酸文库的制备和分析方法 Download PDF

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Publication number
CN113166757A
CN113166757A CN202080006350.2A CN202080006350A CN113166757A CN 113166757 A CN113166757 A CN 113166757A CN 202080006350 A CN202080006350 A CN 202080006350A CN 113166757 A CN113166757 A CN 113166757A
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China
Prior art keywords
target
amplification
primers
primer
wga
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Pending
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CN202080006350.2A
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English (en)
Chinese (zh)
Inventor
伊曼纽尔·坎伯罗夫
木村刚隆
朱莉·凯瑟琳·拉贝特
帕特里克·凯文·马丁
雅各布·梅耶斯
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Bao Bioengineering Usa Co ltd
Takara Bio USA Inc
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Bao Bioengineering Usa Co ltd
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Publication of CN113166757A publication Critical patent/CN113166757A/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080006350.2A 2019-02-15 2020-02-14 核酸文库的制备和分析方法 Pending CN113166757A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962806698P 2019-02-15 2019-02-15
US62/806,698 2019-02-15
PCT/US2020/018360 WO2020168239A1 (en) 2019-02-15 2020-02-14 Methods of preparing and analyzing nucleic acid libraries

Publications (1)

Publication Number Publication Date
CN113166757A true CN113166757A (zh) 2021-07-23

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Family Applications (1)

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CN202080006350.2A Pending CN113166757A (zh) 2019-02-15 2020-02-14 核酸文库的制备和分析方法

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US (1) US20210381035A1 (enExample)
EP (1) EP3924489A4 (enExample)
JP (2) JP7518084B2 (enExample)
CN (1) CN113166757A (enExample)
CA (1) CA3113682A1 (enExample)
WO (1) WO2020168239A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116286714A (zh) * 2022-12-08 2023-06-23 广州市金圻睿生物科技有限责任公司 一种融合型Taq DNA聚合酶及其制备方法和应用
WO2024001404A1 (zh) * 2022-06-27 2024-01-04 北京贝瑞和康生物技术有限公司 检测脆性x综合征突变的方法和试剂盒

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116814766A (zh) * 2023-05-06 2023-09-29 中国人民解放军总医院第一医学中心 基于单细胞全基因组扩增的耳聋无创产前诊断试剂盒

Citations (3)

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JP2006519621A (ja) * 2003-03-07 2006-08-31 ルビコン ゲノミクス, インコーポレイテッド Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析
US20160053328A1 (en) * 2012-12-14 2016-02-25 Chronix Biomedical Personalized biomarkers for cancer
CN109312395A (zh) * 2016-03-31 2019-02-05 珀金埃尔默保健科学(澳大利亚)私人有限公司 靶序列的扩增

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8206913B1 (en) * 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
WO2005118773A2 (en) * 2004-05-28 2005-12-15 Wafergen, Inc. Apparatus and methods for multiplex analyses
US20110033862A1 (en) * 2008-02-19 2011-02-10 Gene Security Network, Inc. Methods for cell genotyping
US20090291475A1 (en) * 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
SG183029A1 (en) * 2009-01-13 2012-08-30 Fluidigm Corp Single-cell nucleic acid analysis
US10004561B2 (en) 2012-10-15 2018-06-26 Life Genetics Lab, Llc Method for genetic detection using interspersed genetic elements: a multiplexed DNA analysis system
US10262755B2 (en) * 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US10208339B2 (en) * 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
US10995370B2 (en) * 2016-09-22 2021-05-04 Invitae Corporation Methods, systems and processes of identifying genetic variations
AU2019359771B2 (en) * 2018-10-17 2025-09-18 Revvity Holdings, Inc. Barcoding of nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006519621A (ja) * 2003-03-07 2006-08-31 ルビコン ゲノミクス, インコーポレイテッド Dna重合プロセスにより生成される全ゲノムおよび全トランスクリプトームライブラリーの増幅および分析
US20160053328A1 (en) * 2012-12-14 2016-02-25 Chronix Biomedical Personalized biomarkers for cancer
CN109312395A (zh) * 2016-03-31 2019-02-05 珀金埃尔默保健科学(澳大利亚)私人有限公司 靶序列的扩增

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024001404A1 (zh) * 2022-06-27 2024-01-04 北京贝瑞和康生物技术有限公司 检测脆性x综合征突变的方法和试剂盒
TWI872585B (zh) * 2022-06-27 2025-02-11 大陸商北京貝瑞和康生物技術有限公司 檢測脆性x綜合征突變的方法和試劑盒
CN116286714A (zh) * 2022-12-08 2023-06-23 广州市金圻睿生物科技有限责任公司 一种融合型Taq DNA聚合酶及其制备方法和应用

Also Published As

Publication number Publication date
JP2022520794A (ja) 2022-04-01
EP3924489A4 (en) 2022-11-23
WO2020168239A1 (en) 2020-08-20
EP3924489A1 (en) 2021-12-22
JP7518084B2 (ja) 2024-07-17
CA3113682A1 (en) 2020-08-20
JP2024147609A (ja) 2024-10-16
US20210381035A1 (en) 2021-12-09

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