CN113149752A - Foliage spraying type agricultural plant enzyme for reducing pesticide residues in agricultural planting - Google Patents
Foliage spraying type agricultural plant enzyme for reducing pesticide residues in agricultural planting Download PDFInfo
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- CN113149752A CN113149752A CN202110233226.6A CN202110233226A CN113149752A CN 113149752 A CN113149752 A CN 113149752A CN 202110233226 A CN202110233226 A CN 202110233226A CN 113149752 A CN113149752 A CN 113149752A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to the technical field of biological fermentation, pesticide and fertilizer reduction and pesticide and fertilizer residue reduction and agricultural product safety, in particular to a foliar spraying type agricultural plant enzyme for reducing pesticide residues in agricultural planting. The innovative technology of the invention is realized according to the following ways: is prepared from fresh fruit, vegetable, plant root, stem and leaf, sugar, agricultural foliage plant ferment strain, water, etc through fermenting for three years and adding strain for seven times. The invention contains various amino acids, trace elements and specific micromolecular bioactive substances, has the effects of improving the conversion and absorption rate of the traditional pesticide and fertilizer, reducing pesticide and fertilizer residues, inhibiting harmful bacteria and the like, and achieves the effects of reducing the use amount of the pesticide and fertilizer and saving labor cost after being fused with the traditional pesticide and fertilizer.
Description
Technical Field
The invention relates to the technical field of biological fermentation, traditional pesticide and fertilizer decrement application, pesticide residue reduction and agricultural product safety, in particular to a foliar spraying type agricultural plant enzyme for reducing pesticide residue in agricultural planting.
Background
When the modern agriculture promotes agricultural products to be greatly increased and the labor productivity is greatly improved, chemical fertilizers and pesticides are excessively used, livestock manure and straws are not properly treated, agricultural non-point source pollution is serious, biological diversity is damaged, soil erosion and hardening are caused, and the quality safety of the agricultural products, the survival safety of human beings and sustainable development are seriously influenced. Under the background of the convergence of resource and environment constraints, the problem to be solved is how to accelerate the agricultural development mode conversion, ensure the effective supply of important agricultural products such as grains and the like, and realize green development and sustainable utilization of resources.
The ferment is a traditional product in China and is a traditional food fermentation technology. As early as 2500 years ago, China has enzyme products produced, and wine residues are used for treating gastrointestinal diseases, which is recorded in emperor's internal classic in detail. In the spring and autumn warring period, the Qin beginner is once the Tai Yi produces the enzyme, and the enzyme is specially used by the self, the queen and the intrauterine personnel to ask for 'longevity'.
Internationally, the plant enzyme has always been regarded as high in importance in the fields of agriculture, feeding, daily use, environmental protection, health preservation and health care and the like. Various plant enzyme industries and plant enzyme products are in endless, plant enzymes form a mature industrial chain, and the enzyme market reaches 1128 billion dollar scale in 2012 worldwide.
In China, the use of agricultural enzymes is just started. Through nine years of intensive experiments and extensive practice, the series products of the 'Fuyuan agricultural plant enzyme' are generally accepted. As a participant of the national plant enzyme industry standard, enterprises integrate development and production, pilot demonstration (standardization) and radiation popularization, and are in a leading position in the industry.
In the traditional agricultural production, in order to stabilize the yield and high yield and ensure the grain production, a large amount of chemical fertilizers and pesticides are used for crops and economic crops in the planting process, and the agricultural materials can pollute the environment in the using process and increase the production cost. Therefore, obtaining the maximum agricultural product profit with the minimum investment of agricultural materials and environmental cost has become an urgent problem to be solved. The agricultural plant enzyme technology in the good margin and the popularization and application of the product can not only reduce the usage amount of pesticide and chemical fertilizer, but also improve the yield and quality of agricultural products, improve the food safety index, reduce the cost of agricultural planting, improve the quality and increase the income, and realize the win-win situation of economic benefit, ecological benefit and social benefit.
With the increasing emphasis on food safety in China, the technical advantages of agricultural enzymes and the market advantages of enzyme agricultural products are increasingly obvious, the bottleneck problem in agricultural development is solved through research, development and application of products, the development requirements of the times are met, and the market requirements are huge. At present, the domestic enzyme industry is in the growth period, the residual error of the product quality is not uniform, the phenomenon of fish eyes mixing with beads is serious, and the public praise of enzyme products in consumers is damaged. Part of the existing products are original and not standard in fermentation process, and the product quality is unstable. The product is developed for eight years, not only belongs to the category of traditional products, but also is an innovative product with high technology content after technical grafting. The method is in the leading position in the world and the country, and fills up the domestic blank. The agricultural foliar plant enzyme strain adopted by the product is prepared by the company through biological purification, permutation and combination and natural fermentation cultivation for hundreds of times in seven years. The leaf surface spraying type agricultural plant enzyme fermented by the method has good fusion, can effectively fuse and use traditional pesticides and fertilizers, and saves labor; the conversion and absorption rate of the pesticide and the chemical fertilizer is improved, and the using amount of the pesticide and the chemical fertilizer is reduced; bacteriostatic action of plant enzyme biodegradation (see figures 1-4); effectively degrading more than 90% of pesticide and fertilizer residues; the CMA pesticide residue detection qualification rate is 100 percent, and the undetected rate is more than 95 percent (see attached figures 5-10).
Disclosure of Invention
The invention relates to a foliage spray type agricultural plant enzyme for reducing pesticide residues in agricultural planting, which takes natural plants as fermentation raw materials and utilizes a plant enzyme fermentation technology to not only have no secondary pollution but also purify the environmental pollution to the ecological environment.
According to the invention, through the biodegradation effect of the plant enzyme, the absorption conversion rate of traditional pesticides and fertilizers in modern agricultural planting is effectively improved, the amount of the pesticides and fertilizers is reduced, the pesticide and fertilizer degrading effect is achieved, and the food control material is safe from the production source. Detection by a third party CMA: the qualified rate of pesticide residues is 100 percent, the undetected rate is more than 95 percent, and the domestic blank is filled.
The technical scheme of the invention is realized as follows: adding natural plant raw materials, sugar and agricultural foliage plant enzyme strains in a fermentation container according to a certain proportion, and naturally fermenting for three years and seven times.
The natural plant raw materials are fresh fruits, vegetables and plant leaves, wherein the fresh fruits comprise 20% of tomato, pepper, cucumber, apple and vegetable green leaves respectively.
The sugar is brown sugar or molasses.
The agricultural foliar plant enzyme strain is prepared by carrying out biological purification, permutation and combination and natural fermentation cultivation for hundreds of times in seven years. The main components are as follows: beneficial ferment microbial flora and derivatives thereof, multiple amino acids and trace elements, IAA synthesis precursor tryptophan, siderophore, phytase, specific micromolecular bioactive substances, polysaccharide, crude polyphenol, amino acid and vitamin (ABCDEK) consisting of yeast flora, bacillus sporophore flora, photosynthetic flora, lactic acid flora, bifidus bacteria, actinomycetes, acetic acid bacteria filamentous fungi and the like.
The plastic container is adopted in the first six times of fermentation of the fermentation container, and because gas can be generated in the fermentation process, the plastic container can be expanded and enlarged, and the safety of the fermentation process is ensured. The seventh fermentation uses stainless steel container.
The process flow (shown in figure 11) of the leaf surface spraying type agricultural plant enzyme fermentation comprises the following steps:
(1) natural plant raw materials, sugar, agricultural foliage plant enzyme strains in the Foyuyuan and water are naturally fermented for 1 month according to the proportion of (1.5-4) to (0.6-1.8) to (0.5-1) to (9-10);
(2) according to the fermentation condition of the plant enzyme fermentation liquor of the first fermentation, the method comprises the following steps: carrying out natural fermentation on the leaf surface spraying type agricultural plant enzyme primary fermentation liquid, sugar and agricultural leaf surface plant enzyme strains in the Foucaila, wherein the natural fermentation period is 3 months in a ratio of 10: 0.5-1.2: 0.5-1;
(3) according to the fermentation condition of the plant ferment fermentation liquor of the secondary fermentation, the method comprises the following steps: carrying out natural fermentation for 8 months by using a leaf surface spraying type agricultural plant enzyme secondary fermentation liquid, sugar and agricultural leaf surface plant enzyme strains in the Foucault land according to the proportion of (9-10) to (0.5-08) to (0.5-1);
(4) according to the fermentation condition of the plant ferment fermentation liquor obtained by the third fermentation, the fermentation conditions are as follows: carrying out natural fermentation for 6 months by using a leaf surface spraying type agricultural plant enzyme three-time fermentation solution, sugar and agricultural leaf surface plant enzyme strains in the Foucaila (L) land in a ratio of (9-10) to (0.5-0.8) to (0.5-1);
(5) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by the four times of fermentation, the following steps are carried out: carrying out natural fermentation for 6 months in a leaf surface spraying type agricultural plant enzyme four-time fermentation liquid, sugar, agricultural leaf surface plant enzyme strains in the Foyuyuan land and water in a ratio of (9-10) to (0.5-1) to (9-10);
(6) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by five times of fermentation, the following steps are carried out: carrying out natural fermentation on the leaf surface spraying type agricultural plant enzyme five-time fermentation liquid, sugar, agricultural leaf surface plant enzyme strains in the Foucaila and water in a ratio of (9-10) to (0.5-1) to (9-10) for 6 months;
(7) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by six times of fermentation, the method comprises the following steps: the leaf surface spraying type agricultural plant enzyme is prepared by naturally fermenting six times of fermentation liquor and agricultural leaf surface plant enzyme strains in the Foucault land in a ratio of (9-10) to (1-2) for 6 months.
Drawings
FIG. 1-FIG. 4 are the bacteriostatic activity experiments of the invention:
1. laboratory apparatus
Constant temperature Oscillator manufacturing of instrument Ltd
Changzhou commercialized Puda teaching apparatus Co., Ltd, electric heating constant temperature incubator
Suzhou Antai air technology Co Ltd of clean workbench Sujing group
Shanghai Shenan medical instrument factory with vertical pressure steam sterilizer
Cascada ultrapure Water System, Germany Pall Corporation
2. Experimental reagents and materials
Absolute ethanol (analytical purity, Kande chemical Co., Ltd., Laiyang City), ultrapure water, LB nutrient agar (HB0129, Haibo Biotechnology Co., Ltd., Hippobo, Hippocampus, Qingdao Gaoko), LB broth (CM158, Beijing land bridge technology Co., Ltd.)
Test bacteria: escherichia coli (CVCC1570/CMCC44102), Bacillus pumilus (CVCC781/CVCC709), Staphylococcus aureus (CVCC1882), Salmonella (salmonella), all provided by the Bionics test center of Qingdao university of agriculture.
3. Experimental procedure
(1) Preparation of the culture Medium
Bacterial liquid culture medium: weighing LB broth 25.0g accurately, placing into conical flask, adding 1L distilled water, boiling to dissolve completely, and subpackaging into 50mL conical flask, 20mL each, and autoclaving at 121 deg.C for 1 h.
Bacteria solid culture medium: accurately weighing 40.0g of LB nutrient agar into a conical flask, adding 1L of distilled water, heating and boiling to completely dissolve, subpackaging into 150mL conical flasks with each flask being 60mL, and autoclaving at 121 ℃ for 1h for later use.
(2) Cultivation of test bacterial suspensions
In an aseptic operating platform, in an alcohol lamp down flame aseptic area, 100uL of stock solution of test strains of escherichia coli, bacillus pumilus, staphylococcus aureus and salmonella is transferred into a conical flask filled with 20mL of liquid culture medium by using a sterilization liquid transfer gun. Sealing with cotton plug, and activating at 37 deg.C in a gas bath constant temperature oscillator, wherein Staphylococcus aureus is activated for 16h, and other bacteria are activated for 8 h.
(3) Preparation of medicated filter paper sheet
Preparing a plurality of filter paper sheets with the diameter of 6mm by using a puncher, and autoclaving at 121 ℃ for 1h for standby. When the bacteriostatic test is carried out, sterilized filter paper sheets are taken by using sterilizing tweezers and placed in a sterilized culture dish, 10 mu L of liquid medicine is transferred to the filter paper sheets by using a liquid transfer gun each time, the filter paper sheets are kept still for a moment to volatilize a solvent, the liquid medicine is repeatedly added twice, the medicine-containing filter paper sheets can be attached to a bacteria-containing flat plate according to the experimental requirements, and the filter paper sheets soaked with distilled water sterilized in equal quantity are used as a control.
(4) Determination of the bacteriostatic Activity
Under aseptic condition, respectively pouring LB nutrient agar culture medium with the temperature of about 60 ℃ into a sterilized culture dish, slowly shaking up, placing in an ultra-clean workbench, and waiting for solidification. In an alcohol burner lower flame sterile area, respectively transferring 100uL of activated bacteria liquid of large intestine bacillus, short bacillus, staphylococcus aureus and salmonella to the center of a solidified culture medium by using a sterilization liquid-transferring gun, and uniformly coating by using a sterilization coater. And marking details by using a marking pen. Then taking three pieces of drug-containing filter paper sheets, and pasting the drug-containing filter paper sheets on the agar surface of a culture dish in a quadrilateral shape by contrasting with one piece of filter paper sheet. And (3) placing the treated culture dish in an incubator at 37 ℃ for culturing for 24h, wherein the staphylococcus aureus needs to be cultured for 48h, and observing and measuring the diameter of the inhibition zone.
4. Results of the experiment
Bacteriostatic effect of foliage-spraying agricultural plant enzyme on bacteria
Note: "+ + + +" indicates high sensitivity (φ)Bacteriostatic ringNot less than 15 mm); "+ +" indicates sensitivity (10 ≦ φ)Bacteriostatic ringLess than or equal to 14 mm); "+" indicates lower sensitivity (φ)Bacteriostatic ringLess than or equal to 10 mm); "-" indicates insensitivity.
FIG. 1 is a test chart of the present invention for inhibiting harmful bacteria, Staphylococcus aureus, in which the upper left corner of the center is a control group and the other three are parallel experimental groups.
FIG. 2 is a test chart of Bacillus pumilus as a harmful bacterium inhibiting agent, wherein the upper left corner of the test chart is a control group, and the other three are parallel test groups.
FIG. 3 is a diagram showing the experimental detection of Escherichia coli, which is a harmful bacterium-inhibiting substance according to the present invention, in which the upper left corner of the diagram is a control group and the remaining three are parallel experimental groups.
FIG. 4 is a diagram showing the experimental detection of the present invention for inhibiting Salmonella which is a harmful bacterium, wherein the upper left corner of the diagram is a control group and the remaining three are parallel experimental groups.
Secondly, FIG. 5-FIG. 10 is the CMA detection report of reducing pesticide residues of the invention:
1. detection mechanism
QINGDAO HARRENS-QAU TESTING Ltd.
2. Detection conclusion
No 63 conventional pesticide residue detections were detected.
FIG. 11 is a flow chart of the biological fermentation process of the present invention
Detailed Description
A foliar-spraying agricultural plant enzyme fermentation process for reducing pesticide residues in agricultural planting comprises the following steps:
(1) natural plant raw materials, sugar, agricultural foliage plant enzyme strains in the Foyuyuan and water are naturally fermented for 1 month according to the proportion of (1.5-4) to (0.6-1.8) to (0.5-1) to (9-10);
(2) according to the fermentation condition of the plant enzyme fermentation liquor of the first fermentation, the method comprises the following steps: carrying out natural fermentation on the leaf surface spraying type agricultural plant enzyme primary fermentation liquid, sugar and agricultural leaf surface plant enzyme strains in the Foucaila, wherein the natural fermentation period is 3 months in a ratio of 10: 0.5-1.2: 0.5-1;
(3) according to the fermentation condition of the plant ferment fermentation liquor of the secondary fermentation, the method comprises the following steps: carrying out natural fermentation for 8 months by using a leaf surface spraying type agricultural plant enzyme secondary fermentation liquid, sugar and agricultural leaf surface plant enzyme strains in the Foucault land according to the proportion of (9-10) to (0.5-08) to (0.5-1);
(4) according to the fermentation condition of the plant ferment fermentation liquor obtained by the third fermentation, the fermentation conditions are as follows: carrying out natural fermentation for 6 months by using a leaf surface spraying type agricultural plant enzyme three-time fermentation solution, sugar and agricultural leaf surface plant enzyme strains in the Foucaila (L) land in a ratio of (9-10) to (0.5-0.8) to (0.5-1);
(5) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by the four times of fermentation, the following steps are carried out: carrying out natural fermentation for 6 months in a leaf surface spraying type agricultural plant enzyme four-time fermentation liquid, sugar, agricultural leaf surface plant enzyme strains in the Foyuyuan land and water in a ratio of (9-10) to (0.5-1) to (9-10);
(6) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by five times of fermentation, the following steps are carried out: carrying out natural fermentation on the leaf surface spraying type agricultural plant enzyme five-time fermentation liquid, sugar, agricultural leaf surface plant enzyme strains in the Foucaila and water in a ratio of (9-10) to (0.5-1) to (9-10) for 6 months;
(7) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by six times of fermentation, the method comprises the following steps: the leaf surface spraying type agricultural plant enzyme is prepared by naturally fermenting six times of fermentation liquor and agricultural leaf surface plant enzyme strains in the Foucault land in a ratio of (9-10) to (1-2) for 6 months.
Claims (6)
1. The utility model provides a fall and subtract incomplete agricultural plant ferment of foliage spray type of farming in farming which characterized in that:
(1) natural plant raw materials, sugar, agricultural foliage plant enzyme strains in the Foyuyuan and water are naturally fermented for 1 month according to the proportion of (1.5-4) to (0.6-1.8) to (0.5-1) to (9-10);
(2) according to the fermentation condition of the plant enzyme fermentation liquor of the first fermentation, the method comprises the following steps: carrying out natural fermentation on the leaf surface spraying type agricultural plant enzyme primary fermentation liquor, sugar and agricultural leaf surface plant enzyme strains in the Fonday land for 3 months at the ratio of 10: 0.5-1.2: 0.5-1;
(3) according to the fermentation condition of the plant ferment fermentation liquor of the secondary fermentation, the method comprises the following steps: carrying out natural fermentation for 8 months by using leaf surface spraying type agricultural plant enzyme secondary fermentation liquor, sugar and agricultural leaf surface plant enzyme strains in the Foucaila alternifolia area according to the proportion of (9-10) to (0.5-08) to (0.5-1);
(4) according to the fermentation condition of the plant ferment fermentation liquor obtained by the third fermentation, the fermentation conditions are as follows: carrying out natural fermentation for 6 months by using leaf surface spraying type agricultural plant enzyme three-time fermentation liquor, sugar and agricultural leaf surface plant enzyme strains in the Foucaila alternifolia area in a ratio of (9-10) to (0.5-0.8) to (0.5-1);
(5) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by the four times of fermentation, the following steps are carried out: the leaf surface spraying type agricultural plant enzyme four-time fermentation liquor, sugar, agricultural leaf surface plant enzyme strains in the Foucaila, and water are naturally fermented for 6 months in a ratio of (9-10) to (0.5-1) to (9-10);
(6) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by five times of fermentation, the following steps are carried out: the leaf surface spraying type agricultural plant enzyme five-time fermentation liquor, sugar, agricultural leaf surface plant enzyme strains in the Foucaila, and water are naturally fermented for 6 months in a ratio of (9-10) to (0.5-1) to (9-10);
(7) according to the fermentation conditions of the plant enzyme fermentation liquor obtained by six times of fermentation, the method comprises the following steps: the leaf surface spraying type agricultural plant enzyme fermentation liquid is fermented for six times, the agricultural leaf surface plant enzyme strain in the Foucaila, the ratio of (9-10) to (1-2) is naturally fermented for 6 months, and the leaf surface spraying type agricultural plant enzyme is prepared.
2. The foliar-spray agricultural plant enzyme for reducing pesticide residues in agricultural planting according to claim 1, wherein: the natural plant raw materials are fresh fruits, vegetables and plant leaves, wherein the fresh fruits comprise 20% of tomato, pepper, cucumber, apple and vegetable green leaves respectively; the sugar is brown sugar or molasses.
3. The foliar-spray agricultural plant enzyme for reducing pesticide residues in agricultural planting according to claim 1, wherein: the agricultural foliar plant enzyme strain is prepared by carrying out biological purification, permutation and combination and natural fermentation cultivation for hundreds of times in seven years. The main components are as follows: beneficial ferment microbial flora and derivatives thereof, multiple amino acids and trace elements, IAA synthesis precursor tryptophan, siderophore, phytase, specific micromolecular bioactive substances, polysaccharide, crude polyphenol, amino acids and vitamins (ABCDEK) consisting of yeast flora, bacillus flora, actinomycetes, lactic acid flora, bifidus, actinomycetes, acetic acid bacteria filamentous fungi and the like.
4. The foliar-spray agricultural plant enzyme for reducing pesticide residues in agricultural planting according to claim 1, wherein the foliar-spray agricultural plant enzyme contains various amino acids and trace elements and specific small-molecule bioactive substances through biodegradation of the plant enzyme, and is applied in combination with pesticide and fertilizer, so that affinity and adhesion between the pesticide and fertilizer and crop leaves can be enhanced, retention time of crops can be prolonged, pesticide targeting can be improved, plant absorption and utilization rate of the pesticide and fertilizer can be significantly improved, and consumption of the pesticide and fertilizer can be reduced by more than 30% while waste of pesticide products is reduced.
5. The foliar spray type agricultural plant enzyme for reducing pesticide residues in agricultural planting according to claim 1, wherein the pesticide residues and the antibiotic residues are effectively degraded through biodegradation of the plant enzyme, particularly hydrolase, phytase, dehydrogenase, monooxygenase, glycosyltransferase and the like which participate in degradation of organophosphorus pesticides, and the reduction removal rate is more than 95%; the food material is controlled to be safe from the production source.
6. The foliar-spray type agricultural plant enzyme for reducing pesticide residues in agricultural planting according to claim 1, wherein the effect of killing harmful bacteria can be achieved through a biological decomposition process by the combined action of a plurality of microorganisms.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113582766A (en) * | 2021-08-31 | 2021-11-02 | 黑龙江省普帅农业科技有限公司 | Preparation method of microbial flora liquid organic fertilizer for removing pesticide residues |
| CN114213191A (en) * | 2021-11-23 | 2022-03-22 | 清大国杰生物科技有限公司 | Agricultural folic acid active mycoprotein polypeptidase plant enzyme, formula and preparation method |
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| CN114213191A (en) * | 2021-11-23 | 2022-03-22 | 清大国杰生物科技有限公司 | Agricultural folic acid active mycoprotein polypeptidase plant enzyme, formula and preparation method |
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Application publication date: 20210723 |