CN113142190B - Liquid nitrogen container for storing cell samples - Google Patents

Liquid nitrogen container for storing cell samples Download PDF

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Publication number
CN113142190B
CN113142190B CN202110504152.5A CN202110504152A CN113142190B CN 113142190 B CN113142190 B CN 113142190B CN 202110504152 A CN202110504152 A CN 202110504152A CN 113142190 B CN113142190 B CN 113142190B
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plate
annular
rod
lifting
driving gear
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CN113142190A (en
Inventor
谭秀文
刘昭华
王可
崔绪奎
王建英
朱荣生
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Institute Animal Science and Veterinary Medicine of Shandong AAS
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Institute Animal Science and Veterinary Medicine of Shandong AAS
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Priority to CN202110504152.5A priority Critical patent/CN113142190B/en
Publication of CN113142190A publication Critical patent/CN113142190A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • A01N1/0257Stationary or portable vessels generating cryogenic temperatures

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  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention provides a liquid nitrogen tank for storing a cell sample, and belongs to the field of cell storage devices. The technical scheme is as follows: a liquid nitrogen tank for storing cell samples comprises a tank body and a lifting basket, wherein the lifting basket comprises a rectangular storage rack and a lifting rod arranged in the middle of one side above the storage rack, the top end of the lifting rod is provided with a limiting rod on the side different from the storage rack, the outer end of the limiting rod is bent downwards, the tank body comprises a tank body with a necking upper end, an annular boss is arranged at the necking part of the tank body, and 5 grooves for placing the limiting rod are uniformly distributed on the annular boss; the lifting mechanism is provided with a clamping rod, and the clamping mechanism can clamp the clamping rod to lift the basket out of the tank body. The invention has the beneficial effects that: the lifting basket can be taken out of the liquid nitrogen tank manually.

Description

Liquid nitrogen container for storing cell samples
Technical Field
The invention relates to the field of cell storage devices, in particular to a liquid nitrogen tank for storing cell samples.
Background
In the existing work of cell cryopreservation and recovery, a liquid nitrogen tank with a low-temperature freezing function is often used for realizing long-term preservation of biological tissue specimens. When the cryopreservation device is used, the cryopreservation tube filled with the cell specimen is generally placed in a basket of a liquid nitrogen tank, and then the basket of the liquid nitrogen tank is placed in the liquid nitrogen tank for long-term low-temperature storage.
When the cells need to be recovered, the basket needs to be taken out from the liquid nitrogen tank manually, and due to the fact that the temperature of the liquid nitrogen is very low, the following problems exist in the process of taking out the basket: certain liquid nitrogen is reserved in the basket, so that the liquid nitrogen is easy to spill out when the liquid nitrogen is lifted out, and certain potential safety hazards are caused to operators; after the basket is lifted, the sealing cover needs to be covered in time so as to cause waste, and when cells are taken out, the sealing cover needs to be opened, so that the operation is complicated; the manual work is in the in-process of carrying out the hand-basket, and the hand is put in jar mouth department all the time, operates like this for a long time, is unfavorable for operator's healthy.
Disclosure of Invention
The invention aims to provide a liquid nitrogen tank for storing cell samples, which has the function of assisting in manually taking out a basket from the liquid nitrogen tank.
The invention is realized by the following measures: a liquid nitrogen tank for storing cell samples comprises a tank body and a lifting basket, wherein the lifting basket comprises a rectangular storage rack and a lifting rod arranged in the middle of one side above the storage rack, the top end of the lifting rod and the different side of the storage rack are provided with limiting rods, the outer end of each limiting rod is bent downwards, the tank body comprises a barrel body with a necking at the upper end, an annular boss is arranged at the necking of the barrel body, 5 grooves for placing the limiting rods are uniformly distributed on the annular boss, and the liquid nitrogen tank is characterized in that the top end of the lifting rod is externally connected with a clamping rod concentric with the lifting rod;
the tank lifting mechanism comprises a frame, a sealing mechanism is arranged on the frame and used for sealing a throwing opening of the tank body, a lifting mechanism is further arranged on the frame and provided with a clamping mechanism of a clamping rod, and the clamping mechanism can clamp the clamping rod and can lift the basket out of the tank body.
The sealing mechanism comprises a hollow annular cylinder concentric with the barrel body, the frame comprises a bottom plate, two ends of one side of the bottom plate are respectively provided with an upright post, the upper end of each upright post is provided with a screw rod, the side wall of the annular cylinder is provided with a support frame, the support frames are sleeved on the screw rods, the lower end of the annular cylinder can be propped against the outer wall of the contraction opening of the barrel body, and the screw rods are provided with nuts for fixing the annular cylinder;
a circular sealing plate consisting of five splicing plates is arranged at the upper end of the annular cylinder and above the annular boss, one end of each splicing plate is hinged above the annular cylinder, a connecting rod is hinged above the other end of each splicing plate, the connecting rods are hinged on a driving gear ring, the driving gear ring and the annular cylinder are concentrically arranged, a first motor with a speed reducer is arranged on the support frame, and a first driving gear meshed with the driving gear ring is arranged on a first motor shaft of the first motor, which extends out of the support frame;
the first motor rotates forwards to drive the first driving gear to rotate, the driving gear drives the driving gear ring to rotate, and the connecting rod on the driving gear ring drives the splicing plates to move, so that the splicing plates are spliced into the circular sealing plate to seal the putting-in opening;
the first motor rotates reversely to drive the first driving gear to rotate, the driving gear drives the driving gear ring to rotate, and the connecting rod on the driving gear ring drives the splicing plates to move, so that one ends of the splicing plates are screwed out of the throwing-in opening, and the throwing-in opening is realized.
The lifting mechanism is an electric push rod, a shell of the electric push rod is arranged on the bottom plate, the tank body is placed on the bottom plate, and the push rod of the electric push rod extends out of the support frame and is provided with the clamping mechanism;
the clamping mechanism comprises a lower horizontal plate arranged on the push rod, a large annular plate is arranged at the outer end of the lower horizontal plate, small annular plates are concentrically arranged on the inner sides of the large annular plates, a connecting plate is arranged above the small annular plates and close to the push rod, the connecting plate is connected with the lower horizontal plate through an upper horizontal plate, the large annular plates and the small annular plates are concentrically arranged with the annular cylinder, an annular track is formed between the large annular plates and the small annular plates at intervals, sliding rods are arranged in the annular track in a sliding mode, a positioning plate is arranged at the upper ends of the sliding rods and above the large annular plates, supporting rods parallel to the tangential direction of the large annular plates are arranged at the lower ends of the sliding rods, side plates are arranged below the two ends of the supporting rods, and a clamping assembly is arranged between the two side plates;
the clamping assembly comprises a rotary table, a hexagonal annular groove is formed in the rotary table, six clamping pieces with the same structure are uniformly distributed in the annular groove, each clamping piece comprises an equilateral triangle and a rectangular plate connected with any one side of the equilateral triangle, a rectangular column matched with the annular groove is arranged on one side of the lower end of the rectangular plate, a limiting column is arranged at the middle position of the upper end of the rectangular plate, a limiting ring plate is arranged above the clamping plate and concentric with the rotary table, a sector ring hole matched with the limiting column is formed in the limiting ring plate, and the limiting ring plate is arranged between the two side plates;
a gear ring II is arranged on the periphery of the turntable, a motor II with a speed reducer is arranged on the limit ring plate, and a motor shaft of the motor II is provided with a driving gear II meshed with the gear ring II;
equilateral triangle is close to the one end in carousel axle center is provided with the arc breach, the carousel rotates, the rectangle post is in it is in simultaneously to remove in the annular spacing post is in the downthehole removal of fan ring, 6 the arc breach of equilateral triangle constitutes the round hole, the storage rack can be followed and put in the mouth and taken out, the round hole with the shadoof can be concentric and press from both sides tightly the shadoof.
The limiting ring plate is rotatably arranged between the two side plates, the positioning plate is rotatably provided with a cam, the limiting ring plate is provided with a vertical plate, and the cam can be propped against the vertical plate to realize the swinging of the limiting ring plate. The cam can be driven by a motor with a speed reducer.
The positioning assembly comprises 5 spring pins uniformly distributed on the inner wall of the large circular plate, positioning holes matched with the spring pins are formed in the sliding rod, and the positions of the spring pins correspond to those of the grooves.
The device also comprises a plunger arranged in the feeding port, and an opening allowing the lifting rod, the limiting rod and the clamping rod to pass through is arranged on the plunger.
The shell of the electric push rod is arranged on a rotating block, and the rotating block is rotatably arranged on the bottom plate.
The supporting frame is provided with a stop lever at any side of the upper horizontal plate, the upper horizontal plate is in contact with the stop lever, and the large circular plate is concentric with the circular cylinder.
The bottom plate is provided with a circular groove for placing the tank body.
The edge of the arc-shaped notch extends downwards to form an arc-shaped clamping plate.
Working principle; the tank body is placed in a circular groove on a bottom plate, so that the frame can be fixed, a lifting basket is placed inside the tank body, a limiting rod is placed in the groove, the plunger is covered to block, the annular cylinder is sleeved on the outer wall of the tank body and the screw rod, the lower part of the annular cylinder is propped against the outer wall of the tank body, the upper end of the annular cylinder is fixed on the screw rod through the nut, the first motor is driven to rotate, the first motor drives the first motor to rotate, the first driving gear drives the driving gear to rotate, the connecting rod on the driving gear drives the splicing plates to move, and the splicing plates are spliced into the circular sealing plate to block the throwing opening;
when the hand-basket is required to be taken out, a motor rotates reversely, drives the driving gear rotates, the driving gear drives the driving gear rotates, the connecting rod on the driving gear drives the splicing plate to move, so that one end of the splicing plate is screwed out the throwing opening is realized, the throwing opening is opened, then the plunger is manually taken out, the hand-basket is manually placed at a position capable of being provided, then the motor is driven, the motor rotates forwards and drives the driving gear to rotate, the driving gear drives the gear to rotate, the turntable rotates and drives the rectangular column to move in the annular, the limiting column is arranged in the fan annular hole to move and 6 the arc-shaped notches of the triangular plate form a round hole, the arc-shaped clamping plate clamps the lifting rod to start the electric push rod to be provided with the lifting basket, and the middle space is far larger than the outer diameter of the lifting rod when the triangular plate is opened, even if the hand-basket is not manually placed at a proper position, the lifting basket can be adjusted through the movement of the triangular plate, and the position of the lifting basket can be ensured.
The lifting basket can be started in the process of lifting the lifting basket, the cam can be driven by a motor with a speed reducer and can be propped against the vertical plate, the limiting ring plate is hinged to the side plate, so that the lifting basket can swing in the tank body, the lifting basket can be conveniently swung, a frozen drawer on the lifting basket can be conveniently swung, the lifting basket can be swung out when being lifted out of the tank body, residual liquid nitrogen in the lifting basket can be swung out and stays for a moment, the motor I is started, the motor I rotates forwards and closes the throwing port, and accordingly consumption of the liquid nitrogen can be avoided.
After the lifting basket is lifted, the clamping mechanism above the electric push rod can be manually screwed out of the upper part of the tank body, so that cells can be conveniently taken out.
In addition, when liquid nitrogen is added at the later stage, the liquid feeding pipe can be clamped through the arc-shaped clamping plate without manually holding the pipeline by people, so that an operator can be far away from the tank body to add the liquid nitrogen, when the tank body is quickly filled, the liquid nitrogen is close to the tank body, and the circular blocking cover can be adjusted, so that the loss of the liquid nitrogen in the adding process can be reduced as far as possible.
Compared with the prior art, the invention has the beneficial effects that: the lifting basket has the function of assisting manual work to take out the lifting basket from the liquid nitrogen tank, reduces the contact of the manual work with the liquid nitrogen in the taking and placing process, reduces the consumption of the liquid nitrogen on one hand, and can prevent the liquid nitrogen from hurting a human body on the other hand; the device can be installed by simply changing the lifting basket and the plunger of the existing liquid nitrogen tank due to small change of the existing liquid nitrogen tank; the device can realize automatic control in the later period according to the prior art.
Drawings
Fig. 1 is a schematic overall structure diagram of an embodiment of the present invention.
Fig. 2 is a schematic structural view of the lifting basket.
Fig. 3 is a partially enlarged view of a point a in fig. 2.
Fig. 4 is a partially enlarged view of fig. 2 at B.
Fig. 5 is a schematic structural diagram of the plugging mechanism.
Fig. 6 is a schematic structural view of the clamping mechanism.
Fig. 7 is a schematic structural view of a turntable related member.
Fig. 8 is a schematic structural view of a tank-related member.
Fig. 9 is a partial enlarged view at C in fig. 8.
Fig. 10 is a schematic structural view of the clamping piece when closed.
Fig. 11 is a schematic view of the structure of the related member of the clamping plate.
Fig. 12 is a schematic view of a structure with a different angle from fig. 6.
Wherein the reference numerals are: 1. a tank body; 2. a storage rack; 3. a base plate; 4. a sealing mechanism; 5. a lifting mechanism; 6. a clamping mechanism; 7. a cam; 8. rotating the block; 9. a plunger; 102. an annular boss; 201. lifting a rod; 202. a limiting rod; 203. a clamping rod; 301. a column; 302. a screw; 303. a nut; 401. an annular cylinder; 402. splicing plates; 403. a connecting rod; 404. a drive gear ring; 405. a support frame; 406. a first motor; 407. a first driving gear; 408. a stop lever; 501. a housing; 502. a push rod; 601. a lower horizontal plate; 602. a large annular plate; 603. a small annular plate; 604. a connecting plate; 605. an upper horizontal plate; 606. a slide bar; 607. a turntable; 608. a ring groove; 609. a rectangular plate; 610. an equilateral triangle; 611. an arc-shaped notch; 612. a fan ring hole; 613. a limiting post; 614. a gear ring II; 615. a second motor; 616. a second driving gear; 617. an arc-shaped splint; 618. a rectangular column; 619. a limit ring plate; 620. a support bar; 701. a third motor; 702. a vertical plate; 901. and (4) opening.
Detailed Description
In order to clearly explain the technical features of the present solution, the present solution is explained by the following detailed description.
Referring to fig. 1-12, a liquid nitrogen tank for storing cell samples comprises a tank body 1 and a lifting basket, wherein the lifting basket comprises a rectangular storage rack 2 and a lifting rod 201 arranged in the middle of one side above the storage rack 2, the top end of the lifting rod 201 and the storage rack 2 are provided with a limiting rod 202 on different sides, the outer end of the limiting rod 202 is bent downwards, the tank body 1 comprises a barrel body with a necking upper end, the necking part of the barrel body is provided with an annular boss 102, 5 grooves for placing the limiting rod 202 are uniformly distributed on the annular boss 102, and the top end of the lifting rod 201 is externally connected with a clamping rod 203 concentric with the lifting rod 201;
still include the frame, be provided with the sealing mechanism 4 of the mouth of puting in of the sealed jar body 21 in the frame, still be provided with elevating system 5 in the frame, be provided with the fixture 6 of supporting rod 203 on the elevating system 5, fixture 6 can centre gripping supporting rod 203 and propose the basket with jar body 1.
The sealing mechanism 4 comprises a hollow annular cylinder 401 concentrically arranged with the barrel body, the frame comprises a bottom plate 3, two ends of one side of the bottom plate 3 are respectively provided with an upright column 301, the upper end of the upright column 301 is provided with a screw 302, the side wall of the annular cylinder 401 is provided with a support frame 405, the support frame 405 is sleeved on the screw 302, the lower end of the annular cylinder 401 can be abutted against the outer wall of the barrel body at the position of the necking, and the screw 302 is provided with a nut 303 for fixing the annular cylinder 401;
a circular sealing plate consisting of five splicing plates 402 is arranged at the upper end of the annular cylinder 401 and above the annular boss 102, one end of each splicing plate 402 is hinged above the annular cylinder 401, a connecting rod 403 is hinged above the other end of each splicing plate 402, the connecting rods 403 are all hinged on a driving gear ring 404, the driving gear ring 404 and the annular cylinder 401 are concentrically arranged, a motor I406 with a speed reducer is arranged on a supporting frame 405, and a driving gear I407 meshed with the driving gear ring 404 is arranged on a motor shaft of the motor I406 extending out of the supporting frame 405;
the first motor 406 rotates forwards to drive the first driving gear 407 to rotate, the first driving gear 407 drives the driving gear ring 404 to rotate, the connecting rod 403 on the driving gear ring 404 drives the splicing plate 402 to move, and the splicing plate 402 is spliced into a circular sealing plate to seal the putting-in opening;
the first motor 406 rotates reversely to drive the first driving gear 407 to rotate, the first driving gear 407 drives the driving gear ring 404 to rotate, the connecting rod 403 on the driving gear ring 404 drives the splicing plate 402 to move, so that one end of the splicing plate 402 is screwed out of the throwing-in opening, and the throwing-in opening is realized.
The lifting mechanism 5 is an electric push rod, a shell 501 of the electric push rod is arranged on the bottom plate 3, the tank body 21 is placed on the bottom plate 3, and a push rod 502 of the electric push rod extends out of the support frame 405 to be provided with a clamping mechanism 6;
the clamping mechanism 6 comprises a lower horizontal plate 601 arranged on the push rod 502, a large annular plate 602 is arranged at the outer end of the lower horizontal plate 601, a small annular plate 603 is concentrically arranged on the inner side of the large annular plate 602, a connecting plate 604 is arranged above the small annular plate 603 and close to the push rod 502, the connecting plate 604 is connected with the lower horizontal plate 601 through an upper horizontal plate 605, the large annular plate 602 and the small annular plate 603 are concentrically arranged with the annular cylinder 401, an annular track is formed by intervals between the large annular plate 602 and the small annular plate 603, a sliding rod 606 is slidably arranged in the annular track, a positioning plate is arranged at the upper end of the sliding rod 606 and above the large annular plate 602, a supporting rod parallel to the tangential direction of the large annular plate 602 is arranged at the lower end of the sliding rod 606, side plates are arranged below the two ends of the supporting rod, and a clamping assembly is arranged between the two side plates;
the clamping assembly comprises a rotary disc 607, a hexagonal ring groove 608 is formed in the rotary disc 607, six clamping pieces with the same structure are uniformly distributed in the ring groove 608, each clamping piece comprises an equilateral triangle 610 and a rectangular plate 609 connected with any one side of the equilateral triangle 610, a rectangular column 618 matched with the ring groove 608 is arranged on one side of the lower end of the rectangular plate 609, a limiting column 613 is arranged in the middle of the upper end of the rectangular plate 609, a limiting ring plate 619 is arranged above the clamping plate, the limiting ring plate 619 and the rotary disc 607 are concentrically arranged, a fan ring hole 612 matched with the limiting column 613 is formed in the limiting ring plate 619, and the limiting ring plate 619 is arranged between the two side plates;
a second gear ring 614 is arranged on the periphery of the turntable 607, a second motor 615 with a speed reducer is arranged on the limit ring plate 619, and a second driving gear 616 meshed with the second gear ring 614 is arranged on a motor shaft of the second motor 615;
the equilateral triangle 610 is provided with an arc notch 611 near the axis of the rotating disc 607, the rotating disc 607 rotates, the rectangular column 618 moves in the ring groove 608 while the limit column 613 moves in the fan ring hole 612, the arc notches 611 of the 6 equilateral triangles 610 form a round hole, the storage rack 2 can be taken out from the putting-in opening, and the round hole and the lifting rod 201 can be concentric and clamp the lifting rod 201.
The stop collar plate 619 rotates and sets up between two curb plates, rotates on the locating plate and is provided with cam 7, is provided with riser 702 on the stop collar plate 619, and cam 7 can push up on riser 702, realizes stop collar plate 619's swing. The cam 7 may be driven by a motor three 701 with a speed reducer.
The positioning device further comprises a positioning assembly of a sliding rod 606, the positioning assembly comprises 5 spring pins which are uniformly distributed on the inner wall of the large annular plate 602, positioning holes matched with the spring pins are formed in the sliding rod 606, and the positions of the spring pins correspond to the positions of the grooves.
The device also comprises a plunger 9 arranged in the throwing opening, wherein the plunger 9 is provided with an opening 901 allowing the lifting rod 201, the limiting rod 202 and the clamping rod 203 to pass through.
The shell 501 of the electric push rod is arranged on the rotating block 8, and the rotating block 8 is rotatably arranged on the bottom plate 3.
A stop lever 408 is arranged on the support frame 405 and positioned at any side of the upper horizontal plate 605, the upper horizontal plate 605 is contacted with the stop lever 408, and the large circular plate 602 is concentric with the circular cylinder 401.
The bottom plate 3 is provided with a circular groove for placing the tank body 21.
The edge of the arc notch 611 extends downwards to form an arc clamping plate 617.
Working principle; the tank body 21 is placed in a circular groove on the bottom plate 3, so that the rack can be fixed, the lifting basket is placed inside the tank body 1, the limiting rod 202 is placed in the groove, the plunger 9 is covered to seal, the annular cylinder 401 is sleeved on the outer wall of the tank body 1 and the screw 302, the lower part of the annular cylinder 401 is propped on the outer wall of the tank body 1, the upper end of the annular cylinder 401 is fixed on the screw 302 through the nut 303, then the motor I406 is driven, the motor I406 drives the motor I406 to rotate forwardly, the driving gear I407 drives the driving gear ring 404 to rotate, the connecting rod 403 on the driving gear ring 404 drives the splicing plate 402 to move, and the splicing plate 402 is spliced into a circular sealing plate to seal a throwing opening;
when the basket needs to be taken out, the motor I406 rotates reversely to drive the driving gear I407 to rotate, the driving gear I407 drives the driving gear ring 404 to rotate, the connecting rod 403 on the driving gear ring 404 drives the splicing plate 402 to move, so that one end of the splicing plate 402 is screwed out of the putting opening, the putting opening is opened, then the plunger 9 is taken out manually, the basket is placed at a position capable of being taken out manually, the motor II 615 is driven, the motor II 615 rotates forwardly to drive the driving gear II 616 to rotate, the driving gear II 616 drives the gear II 614 and the turntable 607 to rotate, the rectangular column 618 is driven to move in the ring groove 608, the limiting column 613 moves in the fan ring hole 612, the arc notches 611 of the 6 equilateral triangular plates 610 form a circular hole, the lifting rod 201 is clamped through the arc clamp plate 617, the electric pushing rod is started to lift the basket, when the 6 equilateral triangular plates 610 are opened, the middle space is far larger than the outer diameter of the lifting rod 201, even if the basket is not placed at a proper position manually, the position of the basket can be adjusted through the movement of the 6 triangular plates 610, and the basket can be taken out.
Mention the in-process of hand-basket, can start cam 7, cam 7 can be through the three 701 drives of motor that have the reduction gear, cam 7 can push up on riser 702, because spacing ring board 619 articulates on the curb plate, can realize that the hand-basket produces the swing in jar body 1 inside, conveniently rock the hand-basket, the drawer that conveniently freezes on the hand-basket shakes out, and can also be when proposing jar body 1 with the hand-basket, rock, shake out remaining liquid nitrogen in the hand-basket, stop for a moment, start motor 406, a motor 406 corotation, close the mouth of putting in, can avoid the consumption of liquid nitrogen like this.
After mentioning the hand-basket, can be manual with fixture on the electric push rod back the top of jar body 1, be about to the hand-basket back-out, conveniently take out the cell.
In addition, when liquid nitrogen is added at the later stage, the liquid feeding pipe can be clamped through the arc-shaped clamping plate 617, the pipeline does not need to be manually held by people, an operator can be far away from the tank body 1 to add the liquid nitrogen, when the tank body is quickly filled, the liquid nitrogen is close to the tank body 1, and the circular blocking cover can be adjusted, so that the loss of the liquid nitrogen in the increasing process can be reduced as far as possible.
It should be noted that the embodiments and features of the embodiments of the present invention may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings, which are merely for convenience in describing the present invention and to simplify the description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, "a plurality" means two or more unless otherwise specified.
In the description of the invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "connected" and "disposed" are to be construed broadly, e.g. as being fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the creation of the present invention can be understood by those of ordinary skill in the art through specific situations.
The technical features of the present invention which are not described in the above embodiments may be implemented by or using the prior art, and are not described herein again, of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and variations, modifications, additions or substitutions which may be made by those skilled in the art within the spirit and scope of the present invention should also fall within the protection scope of the present invention.

Claims (7)

1. A liquid nitrogen tank for storing cell samples comprises a tank body and a lifting basket, wherein the lifting basket comprises a rectangular storage rack and a lifting rod arranged in the middle of one side above the storage rack, the top end of the lifting rod and the different side of the storage rack are provided with limiting rods, the outer end of each limiting rod is bent downwards, the tank body comprises a barrel body with a necking opening at the upper end, an annular boss is arranged at the necking opening of the barrel body, 5 grooves for placing the limiting rods are uniformly distributed on the annular boss, and the top end of the lifting rod is externally connected with a clamping rod concentric with the lifting rod;
the lifting basket lifting device comprises a lifting mechanism, a clamping rod and a lifting mechanism, and is characterized by further comprising a machine frame, wherein a sealing mechanism for sealing a throwing opening of the tank body is arranged on the machine frame, the lifting mechanism is provided with a clamping mechanism for the clamping rod, and the clamping mechanism can clamp the clamping rod to lift the lifting basket out of the tank body;
the sealing mechanism comprises a hollow annular cylinder concentric with the barrel body, the frame comprises a bottom plate, two ends of one side of the bottom plate are respectively provided with an upright post, the upper end of each upright post is provided with a screw rod, the side wall of the annular cylinder is provided with a support frame, the support frames are sleeved on the screw rods, the lower end of the annular cylinder can be propped against the outer wall of the contraction opening of the barrel body, and the screw rods are provided with nuts for fixing the annular cylinder;
a circular sealing plate consisting of five splicing plates is arranged at the upper end of the annular cylinder and above the annular boss, one end of each splicing plate is hinged above the annular cylinder, a connecting rod is hinged above the other end of each splicing plate, the connecting rods are hinged on a driving gear ring, the driving gear ring and the annular cylinder are concentrically arranged, a first motor with a speed reducer is arranged on the support frame, and a first driving gear meshed with the driving gear ring is arranged on the support frame by extending a motor shaft of the first motor out of the support frame;
the first motor rotates forwards to drive the first driving gear to rotate, the driving gear drives the driving gear ring to rotate, and the connecting rod on the driving gear ring drives the splicing plates to move, so that the splicing plates are spliced into the circular sealing plate to seal the putting-in opening;
the first motor rotates reversely to drive the first driving gear to rotate, the driving gear drives the driving gear ring to rotate, and the connecting rod on the driving gear ring drives the splicing plate to move, so that one end of the splicing plate is screwed out of the putting-in opening, and the putting-in opening is opened;
the lifting mechanism is an electric push rod, a shell of the electric push rod is arranged on the bottom plate, the tank body is placed on the bottom plate, and the push rod of the electric push rod extends out of the support frame and is provided with the clamping mechanism;
the clamping mechanism comprises a lower horizontal plate arranged on the push rod, a large annular plate is arranged at the outer end of the lower horizontal plate, small annular plates are concentrically arranged on the inner sides of the large annular plates, a connecting plate is arranged above the small annular plates and close to the push rod, the connecting plate is connected with the lower horizontal plate through an upper horizontal plate, the large annular plates and the small annular plates are concentrically arranged with the annular cylinder, an annular track is formed between the large annular plates and the small annular plates at intervals, sliding rods are arranged in the annular track in a sliding mode, a positioning plate is arranged at the upper ends of the sliding rods and above the large annular plates, supporting rods parallel to the tangential direction of the large annular plates are arranged at the lower ends of the sliding rods, side plates are arranged below the two ends of the supporting rods, and a clamping assembly is arranged between the two side plates;
the clamping assembly comprises a rotary table, a hexagonal annular groove is formed in the rotary table, six clamping pieces with the same structure are uniformly distributed in the annular groove, each clamping piece comprises an equilateral triangle and a rectangular plate connected with any one side of the equilateral triangle, a rectangular column matched with the annular groove is arranged on one side of the lower end of the rectangular plate, a limiting column is arranged at the middle position of the upper end of the rectangular plate, a limiting ring plate is arranged above the clamping plate and concentric with the rotary table, a fan ring hole matched with the limiting column is formed in the limiting ring plate, and the limiting ring plate is arranged between the two side plates;
a gear ring II is arranged on the periphery of the turntable, a motor II with a speed reducer is arranged on the limit ring plate, and a motor shaft of the motor II is provided with a driving gear II meshed with the gear ring II;
an arc-shaped notch is formed in one end, close to the axis of the rotary table, of each equilateral triangular plate, the rotary table rotates, the rectangular columns move in the annular grooves, meanwhile, the limiting columns move in the fan annular holes, the arc-shaped notches of 6 equilateral triangular plates form circular holes, the storage rack can be taken out from the throwing opening, and the circular holes and the lifting rods can be concentric and clamp the lifting rods;
the limiting ring plate is rotatably arranged between the two side plates, the positioning plate is rotatably provided with a cam, the limiting ring plate is provided with a vertical plate, and the cam can be propped against the vertical plate to realize the swinging of the limiting ring plate.
2. The liquid nitrogen tank for storing the cell sample according to claim 1, further comprising a positioning assembly of the slide bar, wherein the positioning assembly comprises 5 spring pins uniformly distributed on the inner wall of the large circular plate, the slide bar is provided with positioning holes matched with the spring pins, and the positions of the spring pins correspond to the positions of the grooves.
3. The liquid nitrogen tank for storing the cell sample according to claim 1, further comprising a plunger disposed in the dispensing port, wherein the plunger is provided with an opening for allowing the lifting rod, the limiting rod and the clamping rod to pass through.
4. The liquid nitrogen tank for storing the cell sample according to claim 1, wherein a housing of the electric push rod is provided on a rotary block, and the rotary block is rotatably provided on the bottom plate.
5. The liquid nitrogen tank for storing the cell sample according to claim 1, wherein a stopper is provided on the support frame on either side of the upper horizontal plate, the upper horizontal plate is in contact with the stopper, and the large circular plate is concentric with the circular cylinder.
6. The liquid nitrogen tank for storing a cell sample according to claim 1, wherein a circular groove for placing the tank body is provided on the bottom plate.
7. The liquid nitrogen tank for storing the cell sample according to claim 1, wherein the edge of the arc-shaped notch extends downward to form an arc-shaped splint.
CN202110504152.5A 2021-05-10 2021-05-10 Liquid nitrogen container for storing cell samples Active CN113142190B (en)

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Publication number Priority date Publication date Assignee Title
KR100843855B1 (en) * 2007-01-18 2008-07-03 주식회사 하이닉스반도체 Semiconductor device and method for fabricating the same
CN204902593U (en) * 2015-07-04 2015-12-23 安徽旭鸿热处理有限公司 Heating furnace bell
CN105004186B (en) * 2015-07-04 2017-04-05 安徽旭鸿热处理有限公司 A kind of heating furnace bell
CN105270865A (en) * 2015-10-27 2016-01-27 芜湖市泰能电热器具有限公司 Annular bar rotating and clamping device
CN105857937B (en) * 2016-04-28 2018-05-08 上海原能健康管理有限公司 Cryopreservation tube access device and liquid nitrogen container access system
CN209749606U (en) * 2019-03-06 2019-12-10 基点生物科技(上海)有限公司 biological sample access arrangement
CN210654208U (en) * 2019-09-20 2020-06-02 上海原能细胞生物低温设备有限公司 System for storing biological samples
CN211059793U (en) * 2019-10-31 2020-07-21 上海康岱生物医药技术股份有限公司 Sealed liquid nitrogen container with clamping and fixing functions
CN212275588U (en) * 2020-04-30 2021-01-01 天津市康婷生物工程集团有限公司 Conversion device for distinguishing fluorescent cells

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