CN113133471A - Compound algistat and preparation method and application thereof - Google Patents
Compound algistat and preparation method and application thereof Download PDFInfo
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
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Abstract
The invention discloses a compound algistat and a preparation method and application thereof, wherein the compound algistat is a slow-release particle, comprises a slow-release particle shell and is composed of chitosan; the nucleation substance is embedded in the center of the sustained-release particles by a sustained-release particle shell and consists of a chemosensory active substance and sodium tripolyphosphate; and sodium nitroprusside loaded on the surface of the slow-release particle shell. The disclosed compound algistat has the advantages of high algae-inhibiting efficiency, long later-period algae-inhibiting time, biodegradability and no pollution, and can be widely applied to water bodies such as ponds, reservoirs, lakes and the like.
Description
Technical Field
The invention belongs to the field of water purification, and particularly relates to an algistat for inhibiting algae in a water body and a preparation method and a use method thereof.
Background
Water is a source of life, is an essential resource for human survival and development, and is closely related to our life. However, in recent years, with the rapid development of the industry in China, the overuse of fertilizers in agriculture and the rapid expansion of cities, the problem of serious water pollution is caused.
The information of Chinese water resource bulletin in 2018 shows that the total amount of water resources in China is 27462.5 hundred million m3The water treatment method mainly comprises the form of surface water resources such as rivers, lakes, reservoirs and the like and underground water resources. The evaluation result of the water resource quality shows that 81.6 percent of river water resources in China belong to I-III water and the water quality is higher, but 58.9 percent of lake water belongs to IV-V class and 16.1 percent of lake water belongs to poor V class for lake water, which indicates that most of lake water in China are seriously polluted, and the main reasons for causing water pollution are concentrated on the problems of ammonia nitrogen and total phosphorus exceeding standard, overlarge chemical oxygen demand, high permanganate index and the like, and the exceeding standard of various nutrient substances in the water and the reduction of dissolved oxygen are bound to cause eutrophication of the water, further cause excessive growth of algae and water quality deterioration. Therefore, inhibition of algae growth is also an important component of water pollution remediation.
Aiming at algae pollution, the aim of algae removal is achieved by adding oxidants such as potassium permanganate and sodium hypochlorite at present. Although the algae removal efficiency of the thermal oxidant is high, the oxidant is easy to cause secondary pollution of water, and the method can only achieve the effect in a short time and cannot achieve the purpose of inhibiting the growth of algae. At present, researches show that chemosensory substances released by aquatic plants have good effects on inhibiting the growth of algae, so that the growth of the algae can be effectively inhibited by adding the chemosensory substances, and other aquatic plants cannot be influenced. However, the slow-release algistat has slow effect and long allelochemical action period, and cannot take effect quickly for the blue-green algae pollution which is fully developed.
Therefore, aiming at the actual algae outbreak situation, the invention provides the compound algae inhibitor, which can achieve the aim of killing algae when algae pollution outbreaks, and can inhibit the growth of subsequent algae through the slow release of allelochemicals.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention discloses a compound algistat which not only has quick algae removal effect, but also has long algae inhibition time in the later period, and can effectively treat water body pollution.
In order to achieve the above purpose, the invention is realized by the following technical scheme; the compound algistat is a slow-release particle, and is characterized by comprising the following components in parts by weight:
a sustained release microparticle shell composed of chitosan;
the nucleation substance is embedded in the center of the sustained-release particles by a sustained-release particle shell and consists of a chemosensory active substance and sodium tripolyphosphate;
and sodium nitroprusside loaded on the surface of the slow-release particle shell.
Preferably, the mass ratio of the allelochemical active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1: 0.8-1.5: 5-15: 1-2.
Preferably, the chemosensory active substance is one or more of vanillic acid, coumaric acid and caffeic acid.
The invention also provides a preparation method of the compound algicide, which is characterized by comprising the following steps:
s1, adding the allelopathy active substance into the sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s2, dissolving chitosan in the acetic acid solution, and adjusting the pH value of the solution to prepare a chitosan acetic acid solution;
s3, adding the nucleating substance mixed solution into the chitosan acetic acid solution, stirring and carrying out ultrasonic treatment to prepare a suspension;
s4, adding sodium nitroprusside into the suspension, stirring, and performing ultrasonic treatment to obtain a sustained-release microparticle suspension;
s5, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s6, adding mannitol into the slow-release particles, and freeze-drying to obtain the compound algicide.
Preferably, the concentration of the sodium tripolyphosphate solution in the step S1 is 0.5 mg/mL-1 mg/mL.
Preferably, the mass concentration of the acetic acid solution in the step S2 is 2% -3%, the adjusted pH value is 4-6, and NaOH solution is adopted for adjusting the pH value; the concentration of chitosan in the chitosan acetic acid solution is 2 mg/mL-3 mg/mL.
Preferably, the nucleating substance mixed solution is added into the chitosan acetic acid solution in the step S3 in a manner that the nucleating substance mixed solution is dropwise added into the chitosan acetic acid solution at a speed of 1-3 mL/min at normal temperature, the stirring speed is 1300-1500 r/min, and the ultrasonic treatment time is 5-10 min.
Preferably, in the step S4, the stirring speed is 1300-1500 r/min, and the ultrasonic treatment time is 5-10 min.
Preferably, the mass ratio of the mannitol to the sustained-release particles in the step S5 is 0.04-0.05: 1.
The invention also provides an application of the compound algicide, which is characterized in that:
s1, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 5-15 mg/L;
s2, performing supplement feeding once a month in the high-temperature period in summer, and performing supplement feeding once every two months in other seasons;
and S3, observing the change of the water body every month, increasing the feeding amount if the algae in the water body is not reduced, and immediately stopping feeding when the protozoon death phenomenon occurs.
Compared with the prior art, the invention has the beneficial effects that:
1. the composite algicide disclosed by the invention has good performances in the aspects of quick algae removal and long-acting algae removal through sodium nitroprusside and allelopathic active substances, and can effectively solve the problem that the slow-release algicide cannot quickly remove algae. The algae inhibiting efficiency of the water body under the condition of algae outbreak is high, the algae inhibiting efficiency is as high as 80 percent, and the duration is long.
2. The chitosan and the sodium tripolyphosphate used in the invention are nontoxic, have good biocompatibility and are biodegradable natural polymer materials, and can effectively avoid secondary pollution in the algae inhibition process. The chemosensory active substance and sodium nitroprusside used in the invention are widely applied in the medical field, so that the chemosensory active substance and sodium nitroprusside have no toxicity to other aquatic organisms.
3. According to the invention, through actually testing the using effect of the algistat, the ideal adding concentration of allelochemicals in the algistat and the ideal adding interval of the algistat are obtained, and a solid and reliable data foundation is provided for subsequent use.
The foregoing description is only an overview of the embodiments of the present invention, and in order to make the technical solutions of the present invention more clear and to implement them in accordance with the contents of the description, the following detailed description of the preferred embodiments of the present invention follows. Specific embodiments of the present invention are given in detail by the following examples.
Detailed Description
The present invention is further described below with reference to specific embodiments for facilitating understanding of those skilled in the art, and it should be noted that any combination between the embodiments or technical features described below may form a new embodiment without conflict.
A composite algistat is a sustained-release particle, and comprises a sustained-release particle shell, a nucleating substance and a water-soluble polymer, wherein the sustained-release particle shell is composed of chitosan, and the nucleating substance with allelopathy is embedded in the center of the sustained-release particle shell and is composed of allelopathy active substances and sodium tripolyphosphate; and sodium nitroprusside loaded on the outer surface of the slow-release particle shell; wherein the mass ratio of the allelochemical active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1: 0.8-1.5: 5-15: 1 to 2.
The allelopathy active substance is one or more of vanillic acid, coumaric acid and caffeic acid.
In the invention, the algistat is a sustained-release particle, a sustained-release particle shell composed of chitosan wraps a allelopathy active substance, and sodium nitroprusside is loaded on the outer surface of the sustained-release particle shell, wherein anions of sodium tripolyphosphate in the nucleating substance are combined with amino cations of chitosan in the sustained-release particle shell through an ionic crosslinking reaction, the sodium nitroprusside is combined with aliphatic primary ammonium in the chitosan through a reaction, and the composite algistat taking the allelopathy substance as a core and the sodium nitroprusside as a load is formed under the combination of two phases.
When the compound algistat is put into a water body, sodium nitroprusside firstly plays a role in destroying the cell structure of algae, so that the activity of the algae is quickly reduced, the settlement of the algae is promoted, and the aim of quickly killing the algae is fulfilled. The allelopathy active substances in the slow-release particles are slowly released in a water body, the process that the aquatic plants slowly release allelopathy substances is simulated, and the activities and functions of various enzymes of algae are influenced in the releasing process of the allelopathy active substances, so that the propagation of the algae is inhibited, and the effect of inhibiting the algae for a long time is further achieved.
The invention also provides a preparation method of the compound algicide, which is characterized by comprising the following steps:
s1, adding the allelopathy active substance into 0.5 mg/mL-1 mg/mL sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s2, dissolving chitosan in an acetic acid solution with the mass concentration of 2-3%, and adjusting the pH value of the acetic acid solution to 4-6 by using NaOH to prepare a chitosan acetic acid solution with the concentration of 2-3 mg/mL;
s3, adding the nucleating substance mixed solution into the chitosan acetic acid solution at the normal temperature at the speed of 1-3 mL/min, stirring at the rotating speed of 1300-1500 r/min, and performing ultrasonic treatment for 5-10 min to obtain a suspension;
s4, adding sodium nitroprusside into the suspension, stirring at the rotating speed of 1300-1500 r/min, and performing ultrasonic treatment for 5-10 min to obtain a sustained-release microparticle suspension;
s5, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s6, adding mannitol into the slow-release particles, and freeze-drying to obtain the compound algicide, wherein the mass ratio of the mannitol to the slow-release particles is 0.04-0.05: 1.
In the step S2, NaOH is used for adjusting pH, the sodium ions are the same as those of sodium nitroprusside, other impurities are not introduced, the reaction of the sodium nitroprusside and the suspension in the step S4 is facilitated, the sodium ions can be removed in the centrifugal process, and convenience is brought to subsequent treatment.
In step S3, the nucleating substance mixed solution is added into the chitosan acetic acid solution at a speed of 1-3 mL/min under a stirring state, so that the slow-release particles wrapping the allelochemicals in the formed suspension are uniform in size. Similarly, in the step S4, the suspension is added to the sodium nitroprusside during the stirring process, so that the distribution of the sodium nitroprusside loaded on the surface of the sustained-release particles is more uniform.
Example 1
A compound algistat is slow release microparticle, comprises slow release microparticle shell composed of chitosan; the nucleating substance is embedded in the center of the sustained-release particles and consists of a chemosensory active substance caffeic acid and sodium tripolyphosphate; and sodium nitroprusside loaded on the outer surface of the slow-release particle shell; wherein the mass ratio of the allelochemical active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1: 0.8: 5: 1.
the compound algistat is prepared by the following preparation method:
s11, adding the allelopathic active substance into 0.5mg/mL sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s12, dissolving chitosan in 2% acetic acid solution, and adjusting the pH value to 6 with NaOH to obtain 2mg/mL chitosan acetic acid solution;
s13, adding the mixed solution of the nucleating substances into the chitosan acetic acid solution at the normal temperature at the speed of 1mL/min, stirring at the rotating speed of 1500r/min, and performing ultrasonic treatment for 10min to obtain a suspension;
s14, adding sodium nitroprusside into the suspension, stirring at the rotation speed of 1500r/min, and performing ultrasonic treatment for 10min to obtain a sustained-release microparticle suspension;
s15, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s16, adding mannitol into the slow-release particles, and freeze-drying the mannitol and the slow-release particles in a mass ratio of 0.04:1 to obtain the compound algicide.
Example 2
A compound algistat is slow release microparticle, comprises slow release microparticle shell composed of chitosan; the nucleating substance is embedded in the center of the sustained-release particles and consists of a chemosensory active substance coumaric acid and sodium tripolyphosphate; and sodium nitroprusside loaded on the outer surface of the slow-release particle shell; wherein the mass ratio of the allelochemical active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1:1.5: 15: 2.
the compound algistat is prepared by the following preparation method:
s21, adding the allelopathy active substance into 1mg/mL sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s22, dissolving chitosan in 3% acetic acid solution, and adjusting the pH value to 4 with NaOH to obtain 3mg/mL chitosan acetic acid solution;
s23, adding the mixed solution of the nucleating substances into the chitosan acetic acid solution at the normal temperature at the speed of 3mL/min, stirring at the rotating speed of 1300r/min, and performing ultrasonic treatment for 5min to obtain a suspension;
s24, adding sodium nitroprusside into the suspension, stirring at the rotation speed of 1300r/min, and performing ultrasonic treatment for 5min to obtain a sustained-release microparticle suspension;
s25, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s26, adding mannitol into the slow-release particles, and freeze-drying the mannitol and the slow-release particles in a mass ratio of 0.05:1 to obtain the compound algistat.
Example 3
A compound algistat is slow release microparticle, comprises slow release microparticle shell composed of chitosan; the nucleating substance is embedded in the center of the sustained-release particles and consists of a chemosensory active substance, namely vanillic acid and sodium tripolyphosphate; and sodium nitroprusside loaded on the outer surface of the slow-release particle shell; wherein the mass ratio of the allelochemical active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1:1: 10: 1.5.
the compound algistat is prepared by the following preparation method:
s31, adding the allelopathic active substance into 0.75mg/mL sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s32, dissolving chitosan in an acetic acid solution with the mass concentration of 2.5%, and adjusting the pH value to 5 by using NaOH to prepare a chitosan acetic acid solution with the concentration of 2.5 mg/mL;
s33, adding the mixed solution of the nucleating substances into the chitosan acetic acid solution at the normal temperature at the speed of 2mL/min, stirring at the rotating speed of 1400r/min, and performing ultrasonic treatment for 7min to obtain a suspension;
s34, adding sodium nitroprusside into the suspension, stirring at the rotating speed of 1400r/min, and performing ultrasonic treatment for 7min to obtain a sustained-release microparticle suspension;
s35, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s36, adding mannitol into the slow-release particles, and freeze-drying the mannitol and the slow-release particles according to the mass ratio of 0.045:1 to obtain the compound algistat.
Example 4
A compound algistat is slow release microparticle, comprises slow release microparticle shell composed of chitosan; the nucleating substance is embedded in the center of the sustained-release particles and consists of chemosensory active substances, namely vanillic acid, coumaric acid and sodium tripolyphosphate; wherein the mass ratio of coumaric acid to vanillic acid is 1:1, and sodium nitroprusside loaded on the surfaces of the sustained-release particles; wherein the mass ratio of the allelochemical active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1:1.2: 12: 1.2.
the compound algistat is prepared by the following preparation method:
s41, adding the allelopathic active substance into 0.8mg/mL sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s42, dissolving chitosan in 2.4% acetic acid solution, and adjusting the pH value to 5 with NaOH to obtain 2.5mg/mL chitosan acetic acid solution;
s43, adding the mixed solution of the nucleating substances into the chitosan acetic acid solution at the normal temperature at the speed of 2mL/min, stirring at the rotating speed of 1400r/min, and performing ultrasonic treatment for 8min to obtain a suspension;
s44, adding sodium nitroprusside into the suspension, stirring at the rotating speed of 1400r/min, and performing ultrasonic treatment for 8min to obtain a sustained-release microparticle suspension;
s45, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s46, adding mannitol into the slow-release particles, and freeze-drying the mannitol and the slow-release particles in a mass ratio of 0.04:1 to obtain the compound algicide.
Comparative example 1:
comparative example 1 sodium nitroprusside was dosed into a water body in the same amount as in example 1.
Comparative example 2
Comparative example 2 caffeic acid was added to a water body in the same amount as in example 1
Comparative example 3
Comparative example 3 is different from example 1 in that step S4 is not included, that is, no sodium nitroprusside is loaded on the surface of the sustained-release particles in the prepared algistat.
The test method comprises the following steps:
a plastic barrel is used as a culture container, aquatic plants and algae are cultured by using normal nutrient solution as a blank control group, except the normal nutrient solution is added into other experimental groups, the algae inhibiting agents in the embodiment and the comparative example are also respectively added, wherein the mass of the compound algae inhibiting agent added in the embodiment is determined by controlling the mass concentration of the allelopathy active substances in the water body, and the mass concentration of the allelopathy active substances in the water body is 10 mg/L; taking example 1 as an example, the compound algicide is added into the water body, the mass concentration of the compound algicide is controlled to be 78mg/L, the culture is continuously carried out for one month, and the growth conditions of algae and aquatic plants are observed.
Test results
The aquatic plants grew normally regardless of the blank control, examples and comparative examples, wherein the algae of the blank control, examples and comparative examples had the following growth conditions compared to the growth conditions after one week and one month from the start of the test:
the invention also provides an application of the compound algicide, and the specific implementation mode adopts the compound algicide prepared in the embodiment 1 in the scheme, and the specific implementation mode is as follows:
example 5:
s51, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 5 mg/L;
s52, putting the compound algistat once at the beginning of 7 months and 8 months respectively;
s53, observing the change of the water body at the bottom of each month; in two months, the normal growth of aquatic plants in the closed water body is not reduced, the original fishes in the water body are not dead or reduced, the algae is reduced by about 70% in comparison with the algae at the beginning of 7 months in the observation of the bottom of 7 months, and the algae is not obviously increased in comparison with the algae at the beginning of 8 months in the observation of the bottom of 8 months.
Example 6
S61, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 5 mg/L;
s62, putting the compound algistat once at the beginning of 7 months and 8 months respectively;
s63, observing the change of the water body at the bottom of each month; in two months, the normal growth of aquatic plants in the closed water body is not reduced, the original fishes in the water body are not dead or reduced, the algae is reduced by about 80% in comparison with the algae at the beginning of 7 months in the observation of the bottom of 7 months, and the algae is reduced by about 5% in comparison with the algae at the beginning of 8 months in the bottom of 8 months.
Example 7
S71, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 15 mg/L;
s72, putting the compound algistat once at the beginning of 7 months and 8 months respectively;
s73, observing the change of the water body at the bottom of each month; in two months, the normal growth of aquatic plants in the closed water body is not reduced, the original fishes in the water body are not dead or reduced, the algae is reduced by about 75% in comparison with the algae at the beginning of 7 months in the observation of the bottom of 7 months, and the algae is not obviously increased in comparison with the algae at the beginning of 8 months in the observation of the bottom of 8 months.
Example 8
S81, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 3 mg/L;
s2, putting the compound algistat once at the beginning of 7 months and 8 months respectively;
s3, observing the change of the water body at the bottom of each month; in two months, the normal growth of aquatic plants in the closed water body is not reduced, the original fishes in the water body are not dead or reduced, the algae is reduced by about 50% in comparison with the algae at the beginning of 7 months in the observation of the bottom of 7 months, and the algae is not obviously increased in comparison with the algae at the beginning of 8 months in the observation of the bottom of 8 months.
Example 9
S91, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 25 mg/L;
s92, putting the compound algistat once at the beginning of 7 months and 8 months respectively;
s93, observing the change of the water body at the bottom of each month; in two months, the normal growth of the aquatic plants in the closed water body is not reduced, the original fishes in the water body are not dead or reduced, the algae is reduced by about 70% in comparison with the algae at the beginning of 7 months in the observation of the bottom of 7 months, the algae is not obviously increased in comparison with the algae at the beginning of 8 months in the bottom of 8 months, but the number of the aquatic plants is relatively reduced by 5%.
Example 10
S101, dispersedly adding a compound algicide into a closed water body; controlling the mass concentration of the chemosensory active substances in the water body to be 10 mg/L;
s2, putting the compound algistat once in the beginning of 7 months;
s3, observing the change of the water body at the bottom of each month; in the two months of 7 and 8, the normal growth of aquatic plants in the closed water body is not reduced, the original fishes in the water body are not dead or reduced, the algae is reduced by about 80% at the beginning of 7 months compared with the algae at the beginning of 8 months, and the algae is increased by 10% at the end of 8 months compared with the algae at the beginning of 8 months.
Example 11
S111, dispersing the compound algicide in a closed water body, and controlling the mass concentration of chemosensory active substances in the water body to be 10 mg/L;
s112, putting the compound algistat once at the beginning of 9 months;
s113, observing the change of the water body at the bottom of each month and month; the normal growth of aquatic plants in the closed water body is not reduced in 9 months and 10 months, the original fishes in the water body are not dead or reduced, the algae is reduced by about 80% in comparison with the algae at the beginning of 9 months in the observation of the end of 9 months, and the algae is not obviously increased in comparison with the algae at the beginning of 10 months in the observation of the end of 10 months.
By comparing the embodiment of the using method of the algistat, the invention discloses the using method of the algistat, and the algistat can obtain the optimal algistatic effect when the concentration of the allelopathic active substance is 5-15 mg/L; the supplementary feeding is carried out once a month in the high-temperature period in summer, and the supplementary feeding is carried out once every two months in other seasons.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner; as will be apparent to those skilled in the art from this disclosure, the present invention may be practiced without these specific details; however, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the scope of the invention as defined by the appended claims; meanwhile, any changes, modifications, and evolutions of the equivalent changes of the above embodiments according to the actual techniques of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (10)
1. A compound algistat is a sustained-release microparticle and is characterized by comprising:
a sustained release microparticle shell composed of chitosan;
the nucleation substance is embedded in the center of the sustained-release particles by a sustained-release particle shell and consists of a chemosensory active substance and sodium tripolyphosphate;
and sodium nitroprusside loaded on the outer surface of the slow-release particle shell.
2. The compound algistat according to claim 1, wherein the mass ratio of the allelochemically active substance to the sodium tripolyphosphate to the chitosan to the sodium nitroprusside is 1: 0.8-1.5: 5-15: 1 to 2.
3. The complex algistat according to claim 1, characterized in that the chemosensory active substance is one or more of vanillic acid, coumaric acid and caffeic acid.
4. A method for preparing the compound algistat according to claim 1, characterized by comprising the steps of:
s1, adding the allelopathy active substance into the sodium tripolyphosphate solution, and uniformly stirring to obtain a nucleating substance mixed solution;
s2, dissolving chitosan in the acetic acid solution, and adjusting the pH value of the solution to prepare a chitosan acetic acid solution;
s3, adding the nucleating substance mixed solution into the chitosan acetic acid solution under the stirring state, and performing ultrasonic treatment to prepare a suspension;
s4, adding sodium nitroprusside into the suspension, stirring, and performing ultrasonic treatment to obtain a sustained-release microparticle suspension;
s5, centrifugally cleaning the sustained-release particle suspension to prepare sustained-release particles;
s6, adding mannitol into the slow-release particles, and freeze-drying to obtain the compound algicide.
5. The method for preparing the composite algistat according to claim 4, wherein the concentration of the sodium tripolyphosphate solution in step S1 is 0.5 mg/mL-1 mg/mL.
6. The preparation method of the compound algistat according to claim 4, characterized in that in step S2, the mass concentration of the acetic acid solution is 2% -3%, the adjusted pH value is 4-6, and NaOH solution is adopted for adjusting the pH value; the concentration of chitosan in the chitosan acetic acid solution is 2 mg/mL-3 mg/mL.
7. The preparation method of the composite algistat according to claim 4, wherein the nucleating substance mixed solution is added to the chitosan acetic acid solution in step S3 by dropping the nucleating substance mixed solution into the chitosan acetic acid solution at a speed of 1-3 mL/min at room temperature, the stirring speed is 1300-1500 r/min, and the ultrasonic treatment time is 5-10 min.
8. The preparation method of the compound algistat according to claim 4, wherein the stirring speed in step S4 is 1300-1500 r/min, and the ultrasonic treatment time is 5-10 min.
9. The method for preparing the compound algistat according to claim 4, wherein the mass ratio of the mannitol to the sustained-release particles in step S6 is 0.04-0.05: 1.
10. The use of the compound algistat according to claim 1, characterized in that:
s1, dispersedly adding the compound algicide into the closed water body, and controlling the mass concentration of the allelopathy active substances in the water body to be 5-15 mg/L;
s2, performing supplement feeding once a month in the high-temperature period in summer, and performing supplement feeding once every two months in other seasons;
and S3, observing the change of the water body every month, increasing the feeding amount if the algae in the water body is not reduced, and immediately stopping feeding when the protozoon death phenomenon occurs.
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