CN113116662A - Low-pressure experimental device - Google Patents
Low-pressure experimental device Download PDFInfo
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- CN113116662A CN113116662A CN202110420311.3A CN202110420311A CN113116662A CN 113116662 A CN113116662 A CN 113116662A CN 202110420311 A CN202110420311 A CN 202110420311A CN 113116662 A CN113116662 A CN 113116662A
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 172
- 239000001301 oxygen Substances 0.000 claims abstract description 172
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 172
- 239000007789 gas Substances 0.000 claims abstract description 33
- 238000004364 calculation method Methods 0.000 claims abstract description 15
- 238000003860 storage Methods 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims description 30
- 238000012937 correction Methods 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 6
- 238000012360 testing method Methods 0.000 abstract description 5
- 230000000740 bleeding effect Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 23
- 206010021143 Hypoxia Diseases 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 238000010926 purge Methods 0.000 description 6
- 239000013589 supplement Substances 0.000 description 6
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000007954 hypoxia Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 231100000255 pathogenic effect Toxicity 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011900 installation process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
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- 230000000474 nursing effect Effects 0.000 description 1
- 208000001797 obstructive sleep apnea Diseases 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
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- 230000005180 public health Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61G—TRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
- A61G10/00—Treatment rooms or enclosures for medical purposes
- A61G10/02—Treatment rooms or enclosures for medical purposes with artificial climate; with means to maintain a desired pressure, e.g. for germ-free rooms
- A61G10/023—Rooms for the treatment of patients at over- or under-pressure or at a variable pressure
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- Health & Medical Sciences (AREA)
- Pulmonology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Accommodation For Nursing Or Treatment Tables (AREA)
Abstract
The application discloses low atmospheric pressure experimental apparatus includes: sealing the observation container; an oxygen detector connected to the closed observation container; an oxygen storage container; the one-way electromagnetic valve is respectively connected with the closed observation container and the oxygen storage container; a pressure detector connected to the closed observation container; the air pump is connected with the closed observation container; the controller is respectively connected with the one-way electromagnetic valve and the air pump; the processor is respectively connected with the controller, the oxygen detector and the pressure detector, and is used for calculating the running time of the air exhauster and the opening and closing time of the one-way electromagnetic valve based on the gas pressure value and the oxygen concentration value and sending the calculation result to the controller; and a control panel connected with the processor. The device can realize that the low-pressure oxygen-poor state can no longer appear after bleeding, and when keeping low pressure, the oxygen content also satisfies the experimental requirement to make the test result more accurate.
Description
Technical Field
The application relates to the field of medical experiments, in particular to a low-pressure experiment device.
Background
The low-pressure pathogenic effect experiment mainly observes the general performance of animals at low pressure and learns the influence of the functional state of the central nervous system on the anoxia tolerance. The low-pressure experimental device is necessary equipment for low-pressure pathogenic effect experiments, and the method of the traditional device is that a vacuum drier is respectively connected with an air extractor and a mercury decompressor by two rubber tubes, a power switch of a switchboard is pressed down, the air extractor is started to enable the air pressure to be respectively and gradually reduced to 507mmHg (equivalent to the height of 3000m at the sea level and the oxygen content of 16.15%), air extraction is stopped, the air extraction is kept for 3-5 minutes, the activity condition of the white mouse, the breathing depth and frequency and the skin color and other indexes of peripheral parts (ear, lip, tail and sole) are observed and recorded, and then a screw clamp on the rubber tubes is released to enable the air pressure to be recovered to be normal. Then, the air pump is started to gradually reduce the air pressure to 270mmHg (corresponding to the altitude of 8000m, the oxygen content of 8.51 percent) and 150mmHg (corresponding to the altitude of 10000m, the oxygen content of 4.95 percent), and the steps are repeated. The disadvantages of this device are: the time for reaching the set air pressure value is different for air extractors with different powers, even if the time for achieving the same set air pressure value by a tiny power error or different working states of the two air extractors with the same power is different, the experimental animals are in oxygen consumption at any time in a closed observation container, the lower the oxygen content in the closed observation container is the longer the air extractor runs, the reduced air pressure value is not proportional to the reduced oxygen content, the more serious oxygen deficiency in the container can be caused by long-time air extraction, the observation conditions among all groups are inconsistent, all indexes are changed, the experiment results among all groups are not comparable, and the statistical significance is not realized. The key point for solving the problem is to set and automatically adjust the air pressure value and the oxygen content so as to ensure that the experimental conditions among the groups are consistent.
Disclosure of Invention
It is an object of the present application to overcome the above problems or to at least partially solve or mitigate the above problems.
According to an aspect of the present application, there is provided a low pressure experimental apparatus, including:
a closed observation container for accommodating an experimental subject;
the oxygen detector is connected with the closed observing container and is used for detecting the oxygen concentration value in the closed observing container;
an oxygen storage container for supplying oxygen to the closed observation container;
the one-way electromagnetic valve is respectively connected with the closed observation container and the oxygen storage container and is used for opening and closing an oxygen outlet of the oxygen storage container;
the pressure detector is connected with the closed observation container and is used for detecting the gas pressure value in the closed observation container;
the air extractor is connected with the closed observation container and used for extracting the gas in the closed observation container;
the controller is respectively connected with the one-way electromagnetic valve and the air pump and is used for controlling the operation of the air pump and the opening and closing of the one-way electromagnetic valve;
the processor is respectively connected with the controller, the oxygen detector and the pressure detector, and is used for calculating the running time of the air exhauster and the opening and closing time of the one-way electromagnetic valve based on the gas pressure value and the oxygen concentration value and sending the calculation result to the controller;
and the control panel is connected with the processor, is used for displaying the gas pressure value and the oxygen concentration value, and is used for receiving a gas pressure set value and an oxygen concentration set value set by a user.
The device can realize no longer appearing the low atmospheric pressure oxygen deficiency state after bleeding, when keeping the low atmospheric pressure, oxygen content also satisfies the experimental requirement, and the device has eliminated the influence that the oxygen volume change brought when testing to the low atmospheric pressure environment to make the test result more accurate.
Optionally, the pressure detector comprises:
the air pressure sensor is used for sensing an air pressure analog signal in the closed observation container;
the first filter is connected with the air pressure sensor and used for filtering the air pressure analog signal;
the first amplifier is connected with the first filter and used for amplifying the air pressure analog signal;
and the first AD converter is connected with the first amplifier and used for converting the air pressure analog signal into the air pressure value and transmitting the air pressure value to the processor.
The pressure detector can accurately detect the air pressure value in the closed observation container.
Optionally, the oxygen detector comprises:
the oxygen sensor is used for sensing an oxygen concentration analog signal in the closed observation container;
the second filter is connected with the oxygen sensor and used for filtering the oxygen concentration analog signal;
the second amplifier is connected with the second filter and used for amplifying the oxygen concentration analog signal;
and the second AD converter is connected with the second amplifier and used for converting the oxygen concentration analog signal into the oxygen concentration value and transmitting the oxygen concentration value to the processor.
Optionally, the processor comprises:
and the oxygen concentration value correction module is respectively connected with the oxygen detector and the pressure detector and is used for correcting the oxygen concentration value based on the gas pressure value.
The device adopts the oxygen concentration value correction module to ensure that the correction value of the oxygen concentration value is real and accurate, thereby providing data basis for the supplement of subsequent oxygen.
Optionally, the oxygen concentration value correction module is configured to: correcting the oxygen concentration value using the following formula:
wherein, OX1Represents an oxygen concentration value; OX2Representing the corrected oxygen concentration value; p1Represents a standard value of air pressure; p2Represents a gas pressure value; k denotes a correction coefficient.
Optionally, the controller is to: and firstly, controlling the air extractor to perform air extraction operation on the closed observation container, and then controlling the one-way electromagnetic valve to be opened and closed.
Therefore, the device firstly exhausts air and then fills oxygen, so that the waste of oxygen can be reduced, the oxygen is saved, and the service life of the device is prolonged.
Optionally, the processor comprises:
the extraction value calculation module is connected with the oxygen detector and used for calculating a first extraction value to be extracted based on the gas pressure value, the gas pressure setting value and the volume of the closed observation container;
the oxygen value calculation module is connected with the oxygen detector and used for calculating a first oxygen amount to be provided based on the oxygen concentration value and the volume of the closed observation container;
the numerical value adjusting module is respectively connected with the controller, the air extraction value calculating module and the oxygen value calculating module, the first air extraction value and the first oxygen amount are adjusted through iterative calculation based on the first air extraction value, the first oxygen amount and the volume of the closed observation container, the second air extraction value and the second oxygen amount are obtained, the adjusted second air extraction value and the adjusted second oxygen amount enable the gas in the closed observation container to meet the gas pressure setting value and the oxygen concentration setting value, the operation time of the air extractor is calculated based on the second air extraction value, the opening and closing time of the one-way electromagnetic valve is calculated based on the second oxygen amount, and the operation time and the opening and closing time are sent to the controller.
The device adopts an iterative calculation mode, repeated air suction and oxygen supplement are not needed, and parameters in the closed observation container can reach a preset target value by one-time air suction and/or one-time oxygen supplement. Therefore, the adjusting time is saved, and the experiment precision is improved.
Optionally, the control panel is configured to receive settings from the user for start-up, stop, extraction rate of the air extractor and air intake rate of the oxygen storage container.
The above and other objects, advantages and features of the present application will become more apparent to those skilled in the art from the following detailed description of specific embodiments thereof, taken in conjunction with the accompanying drawings.
Drawings
Some specific embodiments of the present application will be described in detail hereinafter by way of illustration and not limitation with reference to the accompanying drawings. The same reference numbers in the drawings identify the same or similar elements or components. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale. In the drawings:
FIG. 1 is a schematic block diagram of a low pressure experimental apparatus according to one embodiment of the present application;
FIG. 2 is a schematic block diagram of a pressure detector of the apparatus of FIG. 1;
FIG. 3 is a schematic block diagram of an oxygen detector of the apparatus shown in FIG. 1;
fig. 4 is a schematic block diagram of a processor of the apparatus shown in fig. 1.
Detailed Description
The above and other objects, advantages and features of the present application will become more apparent to those skilled in the art from the following detailed description of specific embodiments thereof, taken in conjunction with the accompanying drawings.
The present application provides a low pressure experimental apparatus 100. Fig. 1 is a schematic structural view of a low pressure experimental apparatus according to an embodiment of the present application. The apparatus may include:
a closed observation container 110 for accommodating an experimental subject;
an oxygen detector 120 connected to the closed observing vessel 110, for detecting an oxygen concentration value in the closed observing vessel 110;
an oxygen storage container 130 for supplying oxygen to the closed observing container 110;
a one-way solenoid valve 140 connected to the closed observing container 110 and the oxygen storage container 130, respectively, for opening and closing an oxygen outlet of the oxygen storage container 130;
a pressure detector 150 connected to the closed observing vessel 110, for detecting a pressure value of the gas in the closed observing vessel 110;
an air extractor 160 connected to the closed observing vessel 110, for extracting air from the closed observing vessel 110;
a controller 170 connected to the one-way solenoid valve 140 and the air extractor 160, respectively, for controlling the operation of the air extractor 160 and the opening and closing of the one-way solenoid valve 140;
a processor 180, respectively connected to the controller 170, the oxygen detector 120, and the pressure detector 150, for calculating an operation time of the aspirator and an opening and closing time of the one-way solenoid valve based on the gas pressure value and the oxygen concentration value, and transmitting the calculation result to the controller 170;
and a control panel 190 connected to the processor 180, for displaying the gas pressure value and the oxygen concentration value, and for receiving a gas pressure setting value and an oxygen concentration setting value set by a user.
The device no longer appears the low atmospheric pressure oxygen deficiency state through bleeding the back, when keeping the low atmospheric pressure, oxygen content also satisfies the experiment requirement, and the device has eliminated the influence that the oxygen volume change brought when testing to the low atmospheric pressure environment to make the test result more accurate.
Fig. 2 is a schematic configuration diagram of a pressure detector of the apparatus shown in fig. 1. Alternatively, the pressure detector 150 may include:
an air pressure sensor 210 for sensing an air pressure analog signal in the closed observation container;
a first filter 220 connected to the air pressure sensor 210, for filtering the air pressure analog signal;
a first amplifier 230 connected to the first filter 220, for amplifying the air pressure analog signal;
and a first AD converter 240, connected to the first amplifier 230, for converting the air pressure analog signal into the air pressure value and transmitting the air pressure value to the processor.
The pressure detector can be arranged on the top cover of the closed observation container so as to prevent the experimental object from damaging and damaging the component. The pressure detector can accurately detect the air pressure value in the closed observation container.
FIG. 3 is a schematic block diagram of an oxygen detector of the apparatus shown in FIG. 1. Optionally, the oxygen detector 110 comprises:
an oxygen sensor 310 for sensing an analog signal of oxygen concentration within the sealed observation container;
a second filter 320 connected to the oxygen sensor 310 for filtering the oxygen concentration analog signal;
a second amplifier 330, connected to the second filter 320, for amplifying the oxygen concentration analog signal;
a second AD converter 340 connected to the second amplifier 330, for converting the oxygen concentration analog signal into the oxygen concentration value, and transmitting the oxygen concentration value to the processor 180.
The oxygen detector can be arranged on the top cover of the closed observation container so as to prevent the experimental object from damaging and damaging the component. The oxygen detector can accurately detect the oxygen concentration value in the closed observation container.
Fig. 4 is a schematic block diagram of a processor of the apparatus shown in fig. 1. Optionally, the processor 180 includes:
an oxygen concentration value correction module 310, connected to the oxygen detector and the pressure detector, respectively, for correcting the oxygen concentration value based on the gas pressure value.
Optionally, the oxygen concentration value correction module is configured to: correcting the oxygen concentration value using the following formula:
wherein, OX1Represents an oxygen concentration value; OX2Representing the corrected oxygen concentration value; p1Represents a standard value of air pressure; p2Indicating gasA pressure value; k denotes a correction coefficient.
Since the measured value of oxygen changes under a low pressure state, the measurement result cannot reflect the actual oxygen concentration in the closed observation container. The device adopts the oxygen concentration value correction module to ensure that the correction value of the oxygen concentration value is real and accurate, thereby providing data basis for the supplement of subsequent oxygen.
Optionally, the controller is to: and firstly, controlling the air extractor to perform air extraction operation on the closed observation container, and then controlling the one-way electromagnetic valve to be opened and closed.
In actual operation, because the oxygen is injected and the air is pumped out, a part of the newly injected oxygen is pumped out along with the oxygen, so that the waste of the oxygen is caused, therefore, the waste of the oxygen can be reduced by pumping the oxygen firstly and then filling the oxygen, the oxygen is saved, and the service life of the device is prolonged.
The controller may include a drive circuit and a relay. The processor controls the on-off of the relay through the driving circuit, and the driving circuit conducts the relay to be closed when a high-level signal is given, so that the starting and stopping of the air extractor are controlled. When the air extractor is started, air in the closed observation container is extracted, so that the air pressure in the container is reduced; when the air extractor stops, if the vent valve is completely closed, the air pressure in the container is observed in a sealed mode and cannot change, and a user can change the air pressure in the container by adjusting a vent valve knob on a controller panel or setting vent to restore the normal value.
Referring to fig. 4, optionally, the processor 180 includes:
the extraction value calculating module 410 is connected to the air pressure detector 150, and is configured to calculate a first extraction value to be extracted based on the air pressure value, the air pressure setting value, and the volume of the closed observation container;
an oxygen value calculating module 420 connected to the oxygen detector 120 or the oxygen concentration value correcting module 310, for calculating a first amount of oxygen to be provided based on the oxygen concentration value and the volume of the sealed observing container;
a numerical adjustment module 430, connected to the controller 170, the purge value calculation module 410, and the oxygen value calculation module 420, respectively, and configured to adjust the first purge value and the first oxygen amount through iterative calculation based on the first purge value, the first oxygen amount, and the volume of the sealed observation container to obtain the second purge value and the second oxygen amount, where the second purge value and the second oxygen amount after adjustment enable the gas in the sealed observation container to satisfy the gas pressure setting value and the oxygen concentration setting value, and calculate an operation time of the gas ejector based on the second purge value, calculate an opening and closing time of the one-way solenoid valve based on the second oxygen amount, and send the operation time and the opening and closing time to the controller.
The device is capable of calculating the extraction and injection of oxygen in an iterative manner, for example, with an oxygen concentration of 30% in the volume of the closed observation vessel. If the amount of extraction and the amount of oxygen are simply calculated as a difference, it is assumed that 200ml of air needs to be extracted from the hermetic vessel so that the air pressure is changed from 507mmHg to 270 mmHg. However, if a certain amount of oxygen is replenished, for example, 60ml, the pressure value will change to over 270mmHg, and at this time, the pressure value and the oxygen value in the closed observation container can reach the experimental standard through repeated operations of re-pumping or even replenishing oxygen again. The device adopts an iterative calculation mode, repeated air suction and oxygen supplement are not needed, and parameters in the closed observation container can reach a preset target value by one-time air suction and/or one-time oxygen supplement. Therefore, the adjusting time is saved, and the experiment precision is improved.
Optionally, the control panel 190 is adapted to receive settings from the user for ventilation, start and stop of the air extractor, air extraction rate, and air intake rate of the oxygen reservoir.
The control panel can be realized by controlling the nixie tube through the single chip microcomputer, and the current value and the set value are displayed in real time in a nixie tube dynamic scanning mode. When the singlechip gives a nixie tube bit selection signal, the nixie tube bit selection signal passes through a 74ls138 decoder and then passes through a buffer stage UN2803 to select one bit of 8-bit nixie tubes to work. The 74ls138 decoder performs 3-line to 8-line decoding, and UN2803 is used for level shifting the inverted control signal and driving the load nixie tube. When the singlechip sends the segment code to be displayed by the nixie tube (namely the binary code of the number to be displayed), the serial input and parallel output of data are realized through 74LS595, the serial input end is a DS pin, and the parallel output bits are Q0 to Q7 and control the display of the nixie tube data through triodes Q2 to Q9.
The control panel can also realize control and setting through the matching of the display screen, the keyboard, the mouse, the buttons and the knobs; it may also be implemented by a touch screen.
The device's airtight effect is outstanding, easy and simple to handle, adopts bayonet type breather valve can resume atmospheric pressure fast.
(1) The joint of the top cover of the closed observation container and the air pump is provided with 3 bayonets, the vent valve is provided with 2 bayonets, and the back of the device is provided with 2 bayonets;
(2) the PU pipe is inserted into the plug, the plug is arranged on the top cover of the closed observation container, when the PU pipe is connected, the PU pipe can be clamped by the bayonet switch only by inserting the PU pipe into the bayonet, the PU pipe is inserted into the bottom of the joint, and the pipe is pulled down after being inserted, so that the pipe can not be pulled out;
(3) when the PU tube is taken down, the bayonet switch is turned on only by pressing down the green clamping cap, and the connection PU tube can be taken down at the moment;
(4) the sign on the vent valve is downward from the fork in the direction of the sealed observation container for the atmosphere.
The installation process of the device is as follows:
(1) coating vacuum grease on the plug when the plug of the closed observation container is installed;
(2) directly inserting a vent valve into the upper end of a plug of the closed observation container;
(3) and (3) coating vacuum grease on the contact edge of a top cover of the closed observation container, and tightly grinding.
(4) Two connectors are arranged on the top cover of the closed observation container in the transverse direction and are respectively connected with an air inlet at the rear end of the device and an air pressure detection port.
The experimental method of the device provided by the application can comprise the following steps: the top cover of the closed observation container is opened, one mouse for birth and one mouse for adult are respectively taken and put into the closed observation container, and the general activity condition, the breathing depth and the frequency of the mice and the skin color of the peripheral parts (ears, lips, tails and soles) of the mice are observed and recorded. The top cover of the closed observation container is covered, and the closed observation container is closed and sealed. The gas pressure and oxygen concentration are set, and the experiment is started to gradually reduce the gas pressure to 507mmHg, 270mmHg, 150mmHg respectively, and the oxygen concentration can be set between 250mg/L and 350mg/L, preferably 310 mg/L.
When the air pressure value reaches the set air pressure value, the air extractor stops working, when the air pressure rises to exceed the set value by 5mmHg, the device automatically enters a program, air is extracted to reduce the air pressure, the low-air-pressure environment is continuously stabilized, when the air pressure is stabilized at the set value, the indexes are observed, and the difference between the two mice is noticed. When the adult rat spasm occurred, the vent valve knob was opened immediately to return the air pressure to normal, the desiccator was opened, and the difference between the two mice was observed.
The device operation interface is simple and practical, and the gassing is safe, and simple operation makes teaching and scientific research more convenient. The observation of animal behavior and body surface change after the air pressure is reduced is also very intuitive, and the air exhaust and air release processes are finished in a short time, so that the experiment becomes easy and quick; the program control air extraction enables the experimental process to be more stable and reliable, has small error and is convenient to obtain objective conclusion. The students can not consume a large amount of time for illegal and invalid operation in the experiment any longer, limited time and energy are applied to the drilling and researching experiment technology, observing the anoxic phenomenon, culturing the practical ability and thinking the anoxic mechanism, and the experiment efficiency and effect are greatly improved. The system can also be used for researching cardiovascular and cerebrovascular diseases for students in the department, designing an oxygen deficiency experiment model and building a brand-new technical platform for implementing scientific and technological innovation projects of college students. The device can also be used for copying relevant scientific research experiments such as models of hypoxia, obstructive sleep apnea syndrome, hypoxia pre-adaptation and the like, and has wide application prospect.
Experiments show that the device innovatively changes a means for copying an integral mammal hypoxia experiment model, and realizes precise adjustment and control of air pressure control. The device has no mercury and is safe to use; the pipeline is closed, the air pressure adjusting range reaches 600mmHg, and the air pressure is stable (the error is less than 5 mmHg); the device adopts the integrated design, high accuracy, and the atmospheric pressure value sets for advantages such as convenient flexibility. The device is used for medical function comprehensive experiments and pharmacological experiment two-door course experiment teaching of 5 colleges of college medical colleges, public health colleges, oral medical colleges, nursing colleges, and students in specialty, and is used for multiple professions and multiple disciplines of schools, and the number of students facing the school and the field reaches 1000 every year. After adopting the device, showing the cultivation that improves practice teaching and to student's hands-on ability, obviously improving the success rate and the experiment teaching efficiency of experiment, still making teacher and student be in a safe experimental environment simultaneously.
Those of skill would further appreciate that the various illustrative components and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware, computer software, or combinations of both, and that the various illustrative components and steps have been described above generally in terms of their functionality in order to clearly illustrate this interchangeability of hardware and software. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the implementation. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present application.
It will be appreciated by those of ordinary skill in the art that the various modules in a processor implementing the embodiments described above may also be implemented by method steps that may be implemented by a program for instructing the processor, where the program may be stored in a computer-readable storage medium, where the storage medium is a non-transitory medium such as a random access memory, a read only memory, a flash memory, a hard disk, a solid state disk, a magnetic tape (magnetic tape), a floppy disk (floppy disk), an optical disk (optical disk), and any combination thereof.
The above description is only for the preferred embodiment of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present application should be covered within the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (5)
1. A low pressure experimental apparatus comprising:
a closed observation container for accommodating an experimental subject;
the oxygen detector is connected with the closed observing container and is used for detecting the oxygen concentration value in the closed observing container;
an oxygen storage container for supplying oxygen to the closed observation container;
the one-way electromagnetic valve is respectively connected with the closed observation container and the oxygen storage container and is used for opening and closing an oxygen outlet of the oxygen storage container;
pressure detector, with airtight observation container is connected for detect the gas pressure value in the airtight observation container, pressure detector includes:
an air pressure sensor for sensing an air pressure analog signal in the closed observation container,
a first filter connected with the air pressure sensor and used for filtering the air pressure analog signal,
a first amplifier connected with the first filter for amplifying the air pressure analog signal,
the first AD converter is connected with the first amplifier and used for converting the air pressure analog signal into the air pressure value and transmitting the air pressure value to the processor;
the air extractor is connected with the closed observation container and used for extracting the gas in the closed observation container;
the controller is respectively connected with the one-way electromagnetic valve and the air pump and is used for controlling the operation of the air pump and the opening and closing of the one-way electromagnetic valve;
a processor respectively connected to the controller, the oxygen detector and the pressure detector, for calculating a running time of the aspirator and opening and closing times of the one-way solenoid valve based on the gas pressure value and the oxygen concentration value, and transmitting the calculation result to the controller, the processor comprising: the oxygen concentration value correction module is respectively connected with the oxygen detector and the pressure detector and is used for correcting the oxygen concentration value based on the gas pressure value; and
the control panel is connected with the processor, is used for displaying the gas pressure value and the oxygen concentration value, and is used for receiving a gas pressure set value and an oxygen concentration set value set by a user;
the oxygen concentration value correction module is used for: correcting the oxygen concentration value using the following formula:
wherein, OX1Represents an oxygen concentration value; OX2Representing the corrected oxygen concentration value; p1Represents a standard value of air pressure; p2Represents a gas pressure value; k denotes a correction coefficient.
2. The apparatus of claim 1, wherein the oxygen detector comprises:
the oxygen sensor is used for sensing an oxygen concentration analog signal in the closed observation container;
the second filter is connected with the oxygen sensor and used for filtering the oxygen concentration analog signal;
the second amplifier is connected with the second filter and used for amplifying the oxygen concentration analog signal; and
and the second AD converter is connected with the second amplifier and used for converting the oxygen concentration analog signal into the oxygen concentration value and transmitting the oxygen concentration value to the processor.
3. The apparatus of claim 1, wherein the controller is configured to: and firstly, controlling the air extractor to perform air extraction operation on the closed observation container, and then controlling the one-way electromagnetic valve to be opened and closed.
4. The apparatus of claim 1, wherein the processor comprises:
the extraction value calculation module is connected with the oxygen detector and used for calculating a first extraction value to be extracted based on the gas pressure value, the gas pressure setting value and the volume of the closed observation container;
the oxygen value calculation module is connected with the oxygen detector and used for calculating a first oxygen amount to be provided based on the oxygen concentration value and the volume of the closed observation container; and
and the numerical value adjusting module is respectively connected with the controller, the air extraction value calculating module and the oxygen value calculating module, the first air extraction value and the first oxygen amount are adjusted through iterative calculation based on the first air extraction value, the first oxygen amount and the volume of the closed observation container to obtain a second air extraction value and a second oxygen amount, the adjusted second air extraction value and the second oxygen amount can enable the gas in the closed observation container to meet the gas pressure setting value and the oxygen concentration setting value, the operation time of the air extractor is calculated based on the second air extraction value, the opening and closing time of the one-way electromagnetic valve is calculated based on the second oxygen amount, and the operation time and the opening and closing time are sent to the controller.
5. The apparatus of any one of claims 1 to 4, wherein the control panel is configured to receive settings from the user for start-up, stop, extraction rate of the aspirator and intake rate of the oxygen reservoir.
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| CN202110420311.3A CN113116662B (en) | 2018-11-27 | 2018-11-27 | Low-pressure experimental device |
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| CN201811429178.2A CN109620597B (en) | 2018-11-27 | 2018-11-27 | Low-pressure experimental device capable of setting and automatically controlling air pressure and oxygen content |
| CN202110420311.3A CN113116662B (en) | 2018-11-27 | 2018-11-27 | Low-pressure experimental device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111454841A (en) * | 2020-05-26 | 2020-07-28 | 上海艾众生物科技有限公司 | Aeration device for a bioreactor |
| CN113520759A (en) * | 2021-06-21 | 2021-10-22 | 浙江科技学院 | Pressure boost oxygenation device for plateau environment |
| CN114322161A (en) * | 2022-01-06 | 2022-04-12 | 中国人民解放军总医院 | Medical shelter integrating ICU (intensive care unit), negative pressure isolation, oxygen-enriched treatment and positive pressure protection functions |
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| CN113116662B (en) | 2022-04-26 |
| CN109620597B (en) | 2021-05-28 |
| CN109620597A (en) | 2019-04-16 |
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