CN113109376B - System and method for transferring sample of cryoelectron microscope and electronic equipment - Google Patents
System and method for transferring sample of cryoelectron microscope and electronic equipment Download PDFInfo
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- CN113109376B CN113109376B CN202110658415.8A CN202110658415A CN113109376B CN 113109376 B CN113109376 B CN 113109376B CN 202110658415 A CN202110658415 A CN 202110658415A CN 113109376 B CN113109376 B CN 113109376B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
- G01N23/2204—Specimen supports therefor; Sample conveying means therefore
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
- G01N23/225—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
- G01N23/2251—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion using incident electron beams, e.g. scanning electron microscopy [SEM]
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/26—Electron or ion microscopes; Electron or ion diffraction tubes
- H01J37/28—Electron or ion microscopes; Electron or ion diffraction tubes with scanning beams
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
- H01J2237/20—Positioning, supporting, modifying or maintaining the physical state of objects being observed or treated
- H01J2237/204—Means for introducing and/or outputting objects
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Abstract
The invention belongs to the technical field of scanning electron microscopes, in particular to a system, a method and electronic equipment for transferring a frozen electron microscope sample, aiming at solving the problem that the frozen electron microscope sample changes in the transferring process; the system comprises a master control center, a transfer table base, a transfer device, a transmission rod, a moving device, a rotating device and a vacuum butt joint mechanism; the moving device comprises a first power device, a first transmission assembly, a transmission screw rod, a transfer rod sliding rail, a moving sliding block and a limiting sliding block, and the rotating device comprises a second power device and a second transmission assembly; a moving assembly consisting of a moving slide block, a limiting slide block and a transmission rod moves to the position where the transmission rod is butted with the transfer device, and the limiting slide block is locked with the second transmission assembly; a rotating assembly consisting of a transmission rod, a limiting slide block and a second transmission assembly rotates to the transmission rod and is locked with the transfer device; the transfer device and the moving assembly move to the vacuum docking mechanism; the invention effectively prevents the structure and the property of the sample from changing in the transferring process.
Description
Technical Field
The invention belongs to the technical field of scanning electron microscopes, and particularly relates to a system and a method for transferring a frozen electron microscope sample and electronic equipment.
Background
The sample chamber of the conventional scanning electron microscope is in a high vacuum environment, the sample is required to be observed to be dry and non-volatile, but many samples contain water, oil or have volatility, so that the use conditions of the conventional scanning electron microscope cannot be met, and meanwhile, the loss of the water, the oil and the like can also cause the change of the structure and the property of the sample, and the distortion of test data. The refrigeration scanning electron microscope developed in recent years makes possible the observation of water-containing and oil-containing samples, such as natural gas hydrate, oil-containing rock, biological cells, suspension, hydrogel and the like, which cannot be observed by a conventional electron microscope, by means of an ultralow-temperature sample stage. When a sample of a frozen scanning electron microscope is prepared, in order to keep the original structure and component characteristics of the sample, a refrigerant is required to be used for quickly freezing the sample, the whole frozen state is kept, and the prepared sample is often exposed to an open environment in the transfer process, so that when the sample is transferred to an electron microscope chamber for observation, the structure and the property of the sample are changed, the characteristics of the sample cannot be ensured, and real data cannot be obtained; in addition, in the transfer process, the transfer table and the transfer rod are clamped in an unstable state, the transfer table for bearing the sample in the transfer process has the problem of instability and falling, the transfer device is difficult to butt with the preparation device, the sample and the instrument are easy to damage, and the quality of the sample in the transfer process cannot be guaranteed.
Disclosure of Invention
In order to solve the problems, namely to solve the problem that the structure and the property of a frozen electron microscope sample are changed in the transfer process and simultaneously overcome the problems that the transfer table in the prior art is unstable, falls off and is difficult to butt joint and collides with an instrument, the invention provides a frozen electron microscope sample transfer system, a method and electronic equipment.
The invention provides a system for transferring a sample of a refrigeration electron microscope, which is used for transferring a prepared refrigeration electron microscope sample out of a cavity of a sample preparation device, wherein the inner bottom wall of the cavity is provided with a mounting groove convenient for transferring the refrigeration electron microscope sample; the vacuum butt-joint mechanism is arranged at the outer side of the chamber to carry the transferred sample.
The sample fixing mechanism comprises a transfer table base and a transfer device; the transfer table base is fixedly arranged on the mounting groove; the transfer device is arranged above the transfer table base and is connected with the transfer table base in a sliding mode.
The transmission mechanism comprises a transmission rod, a moving device and a rotating device, the moving device comprises a first power device, a first transmission assembly, a transmission screw rod, a transfer rod slide rail, a moving slide block and a limiting slide block, the first transmission assembly is arranged at the power output end of the first power device, and the transmission screw rod is arranged at the power output end of the first transmission assembly; the transfer rod slide rail is of a cylindrical structure, and the movable slide block and the limiting slide block are both arranged in the cylindrical structure; the limiting sliding block is rotatably connected with the transfer rod sliding rail, the transmission rod penetrates through the limiting sliding block and is rotatably connected with the moving sliding block, and the transmission rod is fixedly connected with the limiting sliding block; a track connecting part is arranged on the side part of the movable sliding block, and a track clamping part matched with the track connecting part is arranged on the inner wall of the transfer rod sliding rail; a rotary clamping part is arranged on one side, away from the movable sliding block, of the limiting sliding block; and the movable sliding block is provided with a screw rod transmission part matched with the transmission screw rod.
The rotating device comprises a second power device and a second transmission assembly, and the second transmission assembly is arranged at the power output end of the second power device; the second transmission assembly is provided with a rotary butt joint part matched with the rotary clamping part; the transmission rod penetrates through the rotary butt joint part.
In a first working state, the movable sliding block, the limiting sliding block and the transmission rod form a movable assembly; under a second working state, the limiting slide block is clamped and fixed with the second transmission assembly, and the transmission rod, the limiting slide block and the second transmission assembly form a rotating assembly.
In a first working state, the moving assembly is driven by the first power device to move to a first preset position along the longitudinal direction of the transmission rod, the first preset position is that one end, far away from the first power device, of the transmission rod is in butt joint with the transfer device, and the limiting slide block is clamped and fixed with the second transmission assembly; in a second working state, the rotating assembly is driven by the second power device to rotate to a second preset position, and the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device; in a third working state, the transfer device provided with the sample moves away from the chamber of the sample preparation device to the vacuum docking mechanism under the driving of the first power device.
In some preferred embodiments, the top of the transfer table base is provided with a dovetail groove structure groove.
The transfer device comprises a transfer body, a first connecting hole for fixing a sample is formed in the top of the transfer body, and a protruding clamping part matched with the dovetail groove structure groove is formed in the bottom of the transfer body; a second connecting hole for the transmission rod to penetrate through is formed in the front side of the transfer body; the left side and the right side of the transfer body are respectively provided with a third connecting hole and a fourth connecting hole so as to respectively accommodate a first plunger spring main body and a second plunger spring main body, and the first plunger spring main body and the second plunger spring main body are oppositely arranged.
The end part of the first plunger spring body is provided with a first bulge, the end part of the second plunger spring body is provided with a second bulge, and in an extending state, the distance between the first bulge and the second bulge is smaller than the outer diameter of the transmission rod.
In some preferred embodiments, the transfer bar comprises a transfer bar body, a first end for snap-fit connection with the transfer table base, and a second end for rotatable connection with the moving slide.
The first end comprises a first limiting section, a clamping section and a second limiting section, and the clamping section is arranged between the first limiting section and the second limiting section; the outer diameter of the first limiting section is D1, the outer diameter of the clamping section is D2, and the outer diameter of the second limiting section is D3; d1 > D2, and D3 > D2.
The clamping section comprises an arc area and a plane area, the arc area comprises a first arc portion and a second arc portion which are oppositely arranged, the plane area comprises a first plane portion and a second plane portion which are oppositely arranged, and the first arc portion is arranged between the first plane portion and the second plane portion.
In some preferred embodiments, the rail connecting portion includes a movement limiting portion, a first slide rail engaging portion, and a second slide rail engaging portion.
The rail clamping part comprises a rail, a first clamping bulge and a second clamping bulge which are respectively matched with the movement limiting part, the first slide rail clamping part and the second slide rail clamping part.
The movable limiting part is a T-shaped protrusion, and the track is a T-shaped groove.
The first slide rail clamping part is a first groove; the second slide rail clamping part is a second groove.
The longitudinal axis of the T-shaped groove, the longitudinal axis of the first clamping protrusion and the longitudinal axis of the second clamping protrusion are parallel to the longitudinal axis of the transmission rod.
In some preferred embodiments, the lead screw transmission part is a threaded through hole.
One side of the movable sliding block, which is far away from the first power device, is also provided with an elastic part connecting part and a transmission rod connecting part which is rotatably connected with the second end.
A rotary groove is formed in one side, away from the second power device, of the limiting sliding block, an elastic connecting piece is arranged in the rotary groove, and one end, away from the rotary groove, of the elastic connecting piece is fixedly connected with the elastic piece connecting part; one end of the elastic connecting piece, which is far away from the movable sliding block, is connected with the rotating groove in a sliding mode.
The rotary groove is a kidney-shaped groove, and the maximum angle of the elastic connecting piece rotating in the kidney-shaped groove is
The rotation angle of the transmission rod from the first working state to the second working state is
， 
。
In some preferred embodiments, the first transmission assembly comprises a first transmission gear and a second transmission gear, the first transmission gear is fixedly arranged on a power output shaft of the first power device, and the second transmission gear is in meshing transmission with the first transmission gear; one end of the transmission screw rod is fixedly connected with the second transmission gear.
The second transmission assembly comprises a third transmission gear and a fourth transmission gear, the third transmission gear is fixedly arranged on a power output shaft of the second power device, and the fourth transmission gear is in meshing transmission with the third transmission gear.
The rotary butt joint part is arranged on one side of the fourth transmission gear facing the first power device.
The rotating device is arranged on the accommodating shell, and the accommodating shell is arranged on the outer side of the vacuum butt joint mechanism.
In some preferred embodiments, the vacuum docking mechanism comprises a docking fixture snap mechanism for securing connection with an external wall of the sample preparation device and a transfer cartridge housing.
The transfer box shell is arranged between the butt joint fixing clamping mechanism and the accommodating shell; the transfer box shell is provided with a first through hole and a second through hole for the transmission rod to penetrate through, a vacuum gate valve is arranged between the first through hole and the butt joint fixing clamping mechanism to control the connection and disconnection between the interior of the transfer box shell and the butt joint fixing clamping mechanism, and the vacuum gate valve is in signal connection with the master control center; and a magnetic fluid sealing bearing is arranged between the second through hole and the accommodating shell.
And a vacuum pump opening and closing port is formed in the side part of the butt joint fixing and clamping mechanism.
The transfer box comprises a transfer box shell, a heat insulation base and a heat insulation base, wherein the transfer box shell is internally provided with a hollow cavity, and the heat insulation base is arranged on the inner wall of the hollow cavity so as to keep the temperature inside the hollow cavity; and a liquid nitrogen storage tank is arranged in the heat-preservation base so as to adjust the internal temperature of the hollow cavity.
A fixed base is further arranged inside the hollow cavity to limit the transfer device for bearing the sample; the fixed base and the transfer table base are arranged in the same structure.
In some preferred embodiments, the distance from the connecting part of the transmission screw rod and the second transmission gear to the outer wall of the transfer box shell is greater than the length of the transmission screw rod.
The second aspect of the present invention provides a method for transferring a cryo-electron microscope sample, which is based on the cryo-electron microscope sample transfer system described in any of the above, and specifically includes the following steps: step S100, opening a vacuum gate valve arranged in a vacuum butt joint mechanism, starting a first power device, and moving a moving assembly to a first preset position along the longitudinal direction of a transmission rod under the driving of the first power device; the first preset position is that one end of the transmission rod, which is far away from the first power device, is butted with the transfer device, and the limiting slide block is clamped and fixed with the second transmission assembly; step S200, starting a second power device, and rotating the rotating assembly to a second preset position under the driving of the second power device; the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device; step S300, starting a first power device, and moving the transfer device provided with the sample far away from the chamber of the sample preparation device into the chamber of the vacuum docking mechanism under the driving of the first power device; s400, closing a vacuum gate valve arranged in the vacuum butt joint mechanism, and vacuumizing the cavity of the vacuum butt joint mechanism to enable the sample to be in a vacuum environment; step S500, a locking switch of a butt joint fixing clamping mechanism in the vacuum butt joint mechanism is controlled, so that the vacuum butt joint mechanism is separated from the outer side of the cavity of the sample preparation device.
A third aspect of the present invention provides an electronic device comprising: at least one processor; and a memory communicatively coupled to at least one of the processors; wherein the memory stores instructions executable by the processor for execution by the processor to implement the cryo-electron microscope sample transfer method described above.
The system for transferring the sample of the cryogenic electron microscope provided by the invention can effectively ensure the environmental guarantee that the prepared cryogenic electron microscope sample is transferred from the interior of the cavity of the sample preparation device to a corresponding instrument, effectively prevent the temperature rise structure and property change of the sample or severe icing pollution caused by open cryogenic environment transfer, provide a safe and reliable transfer environment, and simultaneously overcome the problems of instability, falling, difficult butt joint and instrument collision in the transfer process of the transfer device in the prior art, so that the transfer process is more accurate and safer.
Drawings
Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings.
Fig. 1 is a schematic perspective view of a sample transfer system for a cryoelectron microscope according to an embodiment of the present invention in an operating state.
Fig. 2 is a cross-sectional view of one embodiment of a cryo-electron microscope sample transfer system in accordance with the present invention.
Fig. 3 is a schematic perspective view of an embodiment of the sample holding mechanism of fig. 2.
FIG. 4 is a perspective view of one embodiment of the sample securing mechanism of FIG. 2.
Fig. 5 is another perspective view of the sample holding mechanism of fig. 2.
Fig. 6 is a partial schematic view of one embodiment of the transfer rod of fig. 2.
Fig. 7 is a schematic perspective view of an embodiment of the movable slider in fig. 2.
Fig. 8 is a schematic perspective view of an embodiment of the limiting slider in fig. 2.
Fig. 9 is a schematic perspective view of an embodiment of the transfer bar slide rail of fig. 2.
Fig. 10 is a perspective view of an embodiment of the fixing base in fig. 2.
Fig. 11 is a partial schematic structural view of the transfer mechanism in fig. 2 in a first operating state.
FIG. 12 is a logic diagram of one embodiment of a cryo-electron microscopy sample transfer method in accordance with the present invention.
The description of the reference numbers follows in order: 100. a sample fixing mechanism; 110. a transfer table base; 120. transfer device, 121, transfer body, 122, first connecting hole, 123, second connecting hole, 124, third connecting hole, 125, first plunger spring body, 126, second plunger spring body, 1261, second protrusion.
200. A transport mechanism; 210. the transmission rod comprises a transmission rod body 211, a first limiting section 212, a clamping section 2121, a clamping part 213, a second limiting section 214 and a transmission rod body; 221. the first power device 222, the first transmission gear 223, the second transmission gear 224 and the transmission screw rod; 225. a transfer bar slide rail, 2251, a first snap projection, 2252, a second snap projection, 2253, a rail; 226. the device comprises a movable sliding block, 2261, a movable limiting part, 2262, a first sliding rail clamping part, 2263, a second sliding rail clamping part, 2264, a transmission rod connecting part, 2265 and a screw rod transmission part; 227. the limiting sliding block is 2271, the rotary clamping part is 2272, and the rotary groove is formed in the limiting sliding block; 228. an elastic connecting member; 231. a second power device 232, a third transmission gear 233 and a fourth transmission gear; 234. accommodating the housing.
300. A vacuum docking mechanism; 310. a butt joint fixing and clamping mechanism; 320. a transfer cassette housing, 321, magnetic fluid sealed bearings; 330. a liquid nitrogen storage tank; 340. a heat-insulating base; 351. a first vacuum gate valve, 352, a second vacuum gate valve; 360. opening and closing a vacuum pump; 370. opening and closing a liquid nitrogen pump; 380. a fixed base; 390. and a liquid nitrogen tank.
400. A sample preparation device.
Detailed Description
In order to make the embodiments, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and are not intended to limit the scope of the present invention.
The invention provides a system for transferring a sample of a refrigeration electron microscope, which is used for transferring a prepared refrigeration electron microscope sample out of a cavity of a sample preparation device, wherein the inner bottom wall of the cavity is provided with a mounting groove convenient for transferring the refrigeration electron microscope sample; the vacuum butt joint mechanism is arranged at the outer side of the chamber to carry the transferred sample; the sample fixing mechanism comprises a transfer table base and a transfer device; the transfer table base is fixedly arranged in the mounting groove; the transfer device is arranged above the transfer table base and is connected with the transfer table base in a sliding mode.
The transmission mechanism comprises a transmission rod, a moving device and a rotating device, the moving device comprises a first power device, a first transmission assembly, a transmission screw rod, a transfer rod slide rail, a moving slide block and a limiting slide block, the first transmission assembly is arranged at the power output end of the first power device, and the transmission screw rod is arranged at the power output end of the first transmission assembly; the transfer rod slide rail is of a cylindrical structure, and the movable slide block and the limiting slide block are both arranged in the cylindrical structure; the limiting slide block is rotatably connected with the transfer rod slide rail, the transmission rod penetrates through the limiting slide block and is rotatably connected with the movable slide block, and the transmission rod is fixedly connected with the limiting slide block; a track connecting part is arranged on the side part of the movable sliding block, and a track clamping part matched with the track connecting part is arranged on the inner wall of the transfer rod sliding rail; one side of the limiting slide block, which is far away from the movable slide block, is provided with a rotary clamping part; the movable sliding block is provided with a screw rod transmission part matched with the transmission screw rod.
The rotating device comprises a second power device and a second transmission assembly, and the second transmission assembly is arranged at the power output end of the second power device; the second transmission assembly is provided with a rotary butt joint part matched with the rotary clamping part; the transmission rod penetrates through the rotary butt joint part; in a first working state, the movable sliding block, the limiting sliding block and the transmission rod form a movable assembly; under the second operating condition, the limiting slide block is clamped and fixed with the second transmission assembly, and the transmission rod, the limiting slide block and the second transmission assembly form a rotating assembly.
In a first working state, the moving assembly is driven by a first power device to move to a first preset position along the longitudinal direction of the transmission rod, the first preset position is that one end, far away from the first power device, of the transmission rod is in butt joint with the transfer device, and the limiting slide block is clamped and fixed with the second transmission assembly; in a second working state, the rotating assembly is driven by a second power device to rotate to a second preset position, and the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device; in a third working state, the transfer device provided with the sample moves away from the chamber of the sample preparation device to the vacuum docking mechanism under the driving of the first power device.
The system for transferring the sample of the cryo-electron microscope disclosed by the invention can ensure the environmental guarantee in the sample transfer process, ensure that the structure and the property of the sample are not changed in the transfer process and ensure the accuracy of test data. In addition, the sample fixing mechanism and the transmission mechanism disclosed in the system can effectively ensure the stable connection of the corresponding devices in the transfer process and effectively prevent the unstable falling of the transfer table in the transfer process; by the arrangement of the transmission mechanism, the butt joint precision in the transfer process is greatly improved, the butt joint and transfer processes are simple and efficient, and the service life of the corresponding device is greatly prolonged.
The invention is further described with reference to the following detailed description of embodiments with reference to the accompanying drawings.
Referring to fig. 1, fig. 2 and fig. 11, fig. 1 is a schematic perspective view of an embodiment of a sample transfer system for a cryo-electron microscope in an operating state, fig. 2 is a cross-sectional view of an embodiment of a sample transfer system for a cryo-electron microscope in the present invention, and fig. 11 is a schematic partial structural view of a transmission mechanism in fig. 2 in a first operating state; the invention provides a system for transferring a sample of a refrigeration electron microscope, which is used for transferring a prepared refrigeration electron microscope sample out of a cavity of a sample preparation device 400, wherein the inner bottom wall of the cavity is provided with a mounting groove convenient for transferring the refrigeration electron microscope sample, the system comprises a master control center, a sample fixing mechanism 100, a transmission mechanism 200 and a vacuum butt joint mechanism 300, and the transmission mechanism and the vacuum butt joint mechanism are in communication connection with the master control center; the vacuum docking mechanism is arranged at the outer side of the chamber to carry the transferred sample.
The sample holding mechanism includes a transfer stage base 110 and a transfer device 120; the transfer table base is fixedly arranged in the mounting groove; the transfer device is arranged above the transfer table base and is connected with the transfer table base in a sliding mode.
The transmission mechanism comprises a transmission rod 210, a moving device and a rotating device, the moving device comprises a first power device 221, a first transmission component, a transmission screw rod 224, a transfer rod slide rail 225, a moving slide block 226 and a limiting slide block 227, the first transmission component is arranged at the power output end of the first power device, and the transmission screw rod is arranged at the power output end of the first transmission component.
The transfer rod slide rail is of a cylindrical structure, and the movable slide block and the limiting slide block are both arranged in the cylindrical structure; the limiting slide block is rotatably connected with the transfer rod slide rail, the transmission rod penetrates through the limiting slide block and is rotatably connected with the movable slide block, and the transmission rod is fixedly connected with the limiting slide block; a track connecting part is arranged on the side part of the movable sliding block, and a track clamping part matched with the track connecting part is arranged on the inner wall of the transfer rod sliding rail; a rotary clamping part 2271 is arranged on one side of the limiting slide block, which is far away from the movable slide block; the movable sliding block is provided with a screw rod transmission part matched with the transmission screw rod.
The rotating device comprises a second power device 231 and a second transmission assembly, and the second transmission assembly is arranged at the power output end of the second power device; the second transmission assembly is provided with a rotary butt joint part matched with the rotary clamping part; the transmission rod penetrates through the rotary butt joint part; the rotating device is installed in the accommodating housing 234, which is installed outside the vacuum docking mechanism.
In a first working state, the movable sliding block 226, the limiting sliding block and the transmission rod 210 form a movable assembly; in a second working state, the limiting slide block is clamped and fixed with the second transmission assembly, and the transmission rod, the limiting slide block and the second transmission assembly form a rotating assembly; in a first working state, the moving assembly is driven by the first power device to move to a first preset position along the longitudinal direction of the transmission rod, the first preset position is that one end, far away from the first power device, of the transmission rod is in butt joint with the transfer device, and the limiting slide block is fixedly clamped with the second transmission assembly; in a second working state, the rotating assembly is driven by a second power device to rotate to a second preset position, and the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device; in a third working state, the transfer device provided with the sample moves away from the chamber of the sample preparation device to the vacuum docking mechanism under the driving of the first power device.
Preferably, when the mounting groove is an inclined groove, the inclined surface of the inclined groove has an inclination angle corresponding to the angle of the sensing rod.
Further, referring to fig. 2 to 5, a dovetail groove structure groove is formed at the top of the transfer table base; the transfer device comprises a transfer body 121, the top of the transfer body is provided with a first connecting hole 122 for fixing a sample, and the bottom of the transfer body is provided with a convex clamping part matched with the groove of the dovetail groove structure; a second connecting hole 123 for the transmission rod to penetrate through is formed in the front side of the transfer body; the left side and the right side of the transfer body are respectively provided with a third connecting hole 124 and a fourth connecting hole to respectively accommodate a first plunger spring main body 125 and a second plunger spring main body 126, and the first plunger spring main body and the second plunger spring main body are oppositely arranged; the end of the first plunger spring body is provided with a first protrusion and the end of the second plunger spring body is provided with a second protrusion 1261, and in the extended state, the distance between the first protrusion and the second protrusion is smaller than the outer diameter of the transmission rod.
Referring to fig. 6 in conjunction with fig. 2, the transfer rod includes a transfer rod body 214, a first end for engaging with the sample base, and a second end for rotatably engaging with the movable slide; the first end comprises a first limit section 211, a clamping section 212 and a second limit section 213, and the clamping section is arranged between the first limit section and the second limit section; the outer diameter of the first limiting section is D1, the outer diameter of the clamping section is D2, and the outer diameter of the second limiting section is D3; d1 > D2, and D3 > D2.
Further, the block section includes circular arc district and plane district, and the circular arc district is including relative first circular arc portion, the second circular arc portion that sets up, and the plane district is including relative first plane portion, the second plane portion that sets up, and first circular arc portion sets up between first plane portion and second plane portion, and first plane portion and second plane portion constitute two block portions 2121 of first plunger spring main part, second plunger spring main part in transmission rod and the transfer device.
In the invention, in a first working state, the moving assembly is driven by the first power device to move to a first preset position along the longitudinal direction of the transmission rod, the first preset position is that one end of the transmission rod, which is far away from the first power device, is butted with the transfer device, the limiting slide block is clamped and fixed with the second transmission assembly, and the butting refers to that two arc areas of the transmission rod are respectively butted with a first bulge of the first plunger spring main body and a second bulge of the second plunger spring main body. In a second working state, the rotating assembly is driven by the second power device to rotate to a second preset position, the second preset position is that one end, far away from the first power device, of the transmission rod is fixedly clamped with the transfer device, in the embodiment, the rotation is 90 degrees, and two plane areas of the transmission rod are respectively abutted against the first protrusion of the first plunger spring body and the second protrusion of the second plunger spring body; through the arrangement of the first limiting section and the second limiting section on the transmission rod, the transmission rod is guaranteed to be clamped and fixed and locked with the transfer device.
Referring to fig. 2 and fig. 7 to 9, fig. 7 is a schematic perspective view of an embodiment of a movable slider in fig. 2, fig. 8 is a schematic perspective view of an embodiment of a position limiting slider in fig. 2, and fig. 9 is a schematic perspective view of an embodiment of a transfer bar slide rail in fig. 2; the rail connecting portion includes a movement limiting portion 2261, a first slide rail engaging portion 2262, and a second slide rail engaging portion 2263; the rail clamping part comprises a rail 2253, a first clamping protrusion 2251 and a second clamping protrusion 2252 which are respectively matched with the movement limiting part, the first slide rail clamping part and the second slide rail clamping part; the moving limiting part is a T-shaped bulge, and the track is a T-shaped groove; the first slide rail clamping part is a first groove; the second slide rail clamping part is a second groove; the longitudinal axis of the T-shaped groove, the longitudinal axis of the first clamping protrusion and the longitudinal axis of the second clamping protrusion are parallel to the longitudinal axis of the transmission rod.
Further, the screw driving part 2265 is a threaded through hole.
One side of the movable sliding block, which is far away from the first power device, is also provided with an elastic connecting piece mounting part and a transmission rod connecting part 2264 which is rotatably connected with the second end of the transmission rod; a rotary groove 2272 is arranged on one side of the limiting slide block, which is far away from the second power device, an elastic connecting piece 228 is arranged in the rotary groove, namely, the elastic connecting piece mounting part is a groove for accommodating the elastic connecting piece, and the convex end of the elastic connecting piece arranged in the groove for moving and sliding is clamped in the rotary groove; in an assembly state, one end of the elastic connecting piece, which is far away from the rotating groove, is fixedly connected with the movable sliding block, and the other end of the elastic connecting piece is connected with the rotating groove in a sliding manner; when the limiting slide block is fixedly clamped with a fourth transmission gear in the rotary transmission assembly, the elastic connecting piece fixedly connected with the movable slide block is arranged, so that the rotary assembly is ensured to rotate for a preset angle relative to the movable slide block, and the fixation of the movable slide block and other assemblies is not influenced.
Preferably, the rotary groove is a kidney-shaped groove, and the maximum angle of rotation of the elastic connecting piece in the kidney-shaped groove is
The rotation angle of the transmission rod from the first working state to the second working state is
， 
。
Preferably, the first and second electrodes are formed of a metal,
。
preferably, the first transmission assembly comprises a first transmission gear 222 and a second transmission gear 223, the first transmission gear is fixedly arranged on the power output shaft of the first power device, and the second transmission gear is in meshing transmission with the first transmission gear; one end of the transmission screw rod is fixedly connected with the second transmission gear; the second transmission component comprises a third transmission gear 232 and a fourth transmission gear 233, the third transmission gear is fixedly arranged on a power output shaft of the second power device, and the fourth transmission gear is in meshing transmission with the third transmission gear; the rotary butt joint part is arranged on one side, in the embodiment, the right side, of the fourth transmission gear facing the first power device.
Referring to fig. 10 and 11 together with fig. 2, the vacuum docking mechanism includes a docking fixture engaging mechanism 310 for fixedly engaging with an outer wall of the sample preparation device and a transfer cassette housing 320; the transfer cassette housing is disposed between the docking fixture and the containment housing 234; the shell of the transfer box is provided with a first through hole and a second through hole for the transmission rod to penetrate through, a vacuum gate valve is arranged between the first through hole and the butt joint fixing clamping mechanism so as to control the connection and disconnection between the inside of the shell of the transfer box and the butt joint fixing clamping mechanism, and the vacuum gate valve is in signal connection with the master control center; a magnetic fluid seal bearing 321 is provided between the second through hole and the accommodating case.
Wherein, the lateral part of the butt joint fixing clamping mechanism is provided with a vacuum pump opening and closing port 360 for vacuumizing the inside of the butt joint fixing clamping mechanism.
The interior of the transfer box shell is a hollow cavity, and the inner wall of the hollow cavity is provided with a heat preservation base 340 so as to keep the temperature inside the hollow cavity; the inside of heat preservation base is provided with liquid nitrogen storage jar 330 to adjust the inside temperature of well cavity, the top of well cavity has seted up liquid nitrogen pump and has opened and close mouth 370 for carry out the transport of liquid nitrogen.
A fixed base 380 is also arranged in the hollow chamber to limit the transfer device for bearing the sample; the fixed base and the transfer table base are arranged in the same structure, namely, a dovetail groove structure groove is formed in the top of the fixed base and matched with the bottom of the transfer device to limit the position in the vertical direction.
Further, the inside of heat preservation base still is provided with liquid nitrogen tank 390, and the liquid nitrogen tank through encircleing vacuum sample storehouse can be fast better refrigeration, guarantees to transmit to the temperature regulation and control of the inside sample in cavity, and further, in order to keep the temperature of fixed base and transfer device, makes the sample that shifts here be in preset environment.
Preferably, the distance from the connecting part of the transmission screw rod and the second transmission gear to the outer wall of the shell of the transfer box is greater than the length of the transmission screw rod.
Preferably, the distance D1 traveled by the snap-fit section on the transfer lever is equal to the distance D2 between the transfer stage base in the sample holding mechanism and the holding base in the vacuum docking mechanism.
Further, the distance from the side, away from the sample preparation device, of the fourth gear in the rotary transmission assembly to the side, away from the first power device, of the second gear in the movable transmission assembly is D1, the size of the movable sliding block in the longitudinal axial direction of the transmission rod is D2, the size of the limiting sliding block in the longitudinal axial direction of the transmission rod is D3, and D1 is greater than D1+ D2+ D3, so that the movable distance of the movable sliding block is greater than the movement distance of the clamping section.
Furthermore, the distance from the left side surface of the transmission rod to the right side surface of the movable sliding block is d4, the distance from the left side surface of the fixed base in the vacuum butt joint mechanism to the left side surface of the first gear in the movable transmission assembly is d5, and d5 is greater than d4, so that when the transmission rod drives the transfer device to move into the vacuum butt joint mechanism to be clamped with the fixed base, the length of the transfer rod sliding rail can accommodate the transmission rod.
Further, the length of the first limiting section is L1, the length of the engaging section is L2, the length of the second limiting section is L3, the length of the second connecting hole in the transfer table base in the sample fixing mechanism is L4, and L4= L1+ L2+ L3.
Further, the size of the first bulge in the longitudinal direction of the transmission rod is smaller than the aperture of the third connecting hole; the aperture of the third connecting hole is equal to the length of the clamping section.
Further, the dimension of the first projection in the longitudinal direction of the transmission rod is equal to the dimension of the engaging portion in the longitudinal direction of the transmission rod.
Further, in the initial state, the left end of the transmission rod is aligned with the left end of the fixed base, and the left side of the transfer device is aligned with the left side of the fixed base; the distance that the limiting slide block moves from the initial state position to the position completely clamped with the fourth transmission gear in the rotary transmission assembly is equal to the distance that the transmission rod moves from the initial position of the fixed base to the position butted with the sample table base, and when the transmission rod is in the butted state with the sample table base, the left end part of the transmission rod is aligned with the left end part of the sample table base.
And the distance from the leftmost side of the transmission rod to the center of the clamping section of the transmission rod is equal to the distance from the left side of the transfer device to the center of the plunger spring mounting hole.
Preferably, the vacuum gate valve comprises a first vacuum gate valve 351 and a second vacuum gate valve 352, the first vacuum gate valve is arranged in the butt joint fixing clamping mechanism, the second vacuum gate valve is arranged between the transfer box shell and the butt joint fixing clamping mechanism, namely the first vacuum gate valve and the second vacuum gate valve are respectively arranged at two ends of the opening and closing port of the vacuum pump, and the adjustment of the internal vacuum degree of the butt joint fixing clamping mechanism can be carried out through the arrangement of the first vacuum gate valve and the second vacuum gate valve.
Referring to FIG. 12, a logic diagram of one embodiment of a cryo-electron microscopy sample transfer method of the present invention is shown; the second aspect of the invention provides a method for transferring a sample for a cryo-electron microscope, which is based on the system for transferring a sample for a cryo-electron microscope and specifically comprises the following steps: step S100, opening a vacuum gate valve arranged in a vacuum butt joint mechanism, namely opening a first vacuum gate valve and a second vacuum gate valve, starting a first power device, and moving a moving assembly to a first preset position along the longitudinal direction of a transmission rod under the driving of the first power device; the first preset position is that one end of the transmission rod, far away from the first power device, is in butt joint with the transfer device, and the limiting slide block is clamped and fixed with the second transmission assembly.
Step S200, starting a second power device, and rotating the rotating assembly to a second preset position under the driving of the second power device; the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device; in this embodiment, rotated 90 °, the two planar zones of the transfer rod respectively abut against the first protrusion of the first plunger spring body and the second protrusion of the second plunger spring body; through the arrangement of the first limiting section and the second limiting section on the transmission rod, the transmission rod is guaranteed to be clamped and fixed and locked with the transfer device.
Step S300, starting a first power device, and moving the transfer device provided with the sample far away from the chamber of the sample preparation device into the chamber of the vacuum docking mechanism under the driving of the first power device; specifically, the transfer device is driven by the transmission rod to move to be clamped with the fixed base.
S400, closing a vacuum gate valve arranged in the vacuum butt joint mechanism, and vacuumizing the cavity of the vacuum butt joint mechanism to enable the sample to be in a vacuum environment; specifically, the vacuum gate valve close to one side of the sample preparation device, namely the first vacuum gate valve, is closed first to be vacuumized, and then the vacuum gate valve close to one side of the first power device, namely the second vacuum gate valve, is closed after the vacuumizing is completed, so that the vacuum environment adjustment of the space where the sample is located is completed.
Step S500, controlling a locking switch of a butt joint fixing clamping mechanism in the vacuum butt joint mechanism to enable the vacuum butt joint mechanism to be separated from the outer side of a cavity of the sample preparation device; specifically, the first vacuum gate valve and the second vacuum gate valve are closed, air or argon is filled into the butt joint fixing and clamping mechanism, and a locking switch of the butt joint fixing and clamping mechanism in the vacuum butt joint mechanism is controlled, so that the vacuum butt joint mechanism is separated from the outer wall of the sample preparation device to form a movable transfer system.
Further, the transfer device is transferred to the next instrument according to the requirement, after the transfer device is moved to the next instrument and can be in butt joint and vacuumized, the vacuum gate valve is opened, and the first power device drives the transmission rod to separate the transfer device from the fixed base and install the transfer device into the base of the transfer table of the corresponding instrument; after the sample is loaded, the transmission rod is controlled to rotate by 90 degrees through the rotating device so as to be unlocked with the transfer device for fixing the sample, the first power device drives the transmission rod to be recovered into the transfer rod slide rail of the moving device, and the vacuum gate valve is closed to finish the transfer to another instrument.
According to the invention, the first power device is indirectly connected with the transmission rod, so that the outer diameter of the sliding rail of the transfer rod for accommodating the transmission rod is smaller, and the sliding rail is convenient to grasp during transfer.
A third aspect of the present invention provides an electronic device comprising: at least one processor; and a memory communicatively coupled to at least one of the processors; wherein the memory stores instructions executable by the processor for execution by the processor to implement the cryo-electron microscope sample transfer method.
It should be noted that in the description of the present invention, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicating the directions or positional relationships are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The terms "comprises," "comprising," or any other similar term are intended to cover a non-exclusive inclusion, such that a process, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, article, or apparatus.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
Claims (9)
1. A kind of sample transfer system of cryo-electron microscope, this system is used for preparing the cryo-electron microscope sample finished and turning out from the cavity inside of the sample preparation device, wherein, the inner bottom wall of the cavity has mounting grooves convenient for cryo-electron microscope sample to transfer, characterized by that, this system includes total control center, sample fixed organization, transport mechanism and vacuum docking mechanism, said transport mechanism, said vacuum docking mechanism all with said total control center communication connection; the vacuum butt joint mechanism is arranged at the outer side of the chamber to carry the transferred sample;
the sample fixing mechanism comprises a transfer table base and a transfer device; the transfer table base is fixedly arranged on the mounting groove; the transfer device is arranged above the transfer table base and is connected with the transfer table base in a sliding mode; a dovetail groove structure groove is formed in the top of the transfer table base; the transfer device comprises a transfer body, a first connecting hole for fixing a sample is formed in the top of the transfer body, and a protruding clamping part matched with the dovetail groove structure groove is formed in the bottom of the transfer body; a second connecting hole for the transmission rod to penetrate through is formed in the front side of the transfer body; the left side and the right side of the transfer body are respectively provided with a third connecting hole and a fourth connecting hole so as to respectively accommodate a first plunger spring main body and a second plunger spring main body, and the first plunger spring main body and the second plunger spring main body are oppositely arranged; a first bulge is arranged at the end part of the first plunger spring body, a second bulge is arranged at the end part of the second plunger spring body, and the distance between the first bulge and the second bulge is smaller than the outer diameter of the transmission rod in an extending state;
the transmission mechanism comprises a transmission rod, a moving device and a rotating device, the moving device comprises a first power device, a first transmission assembly, a transmission screw rod, a transfer rod slide rail, a moving slide block and a limiting slide block, the first transmission assembly is arranged at the power output end of the first power device, and the transmission screw rod is arranged at the power output end of the first transmission assembly; the transfer rod slide rail is of a cylindrical structure, and the movable slide block and the limiting slide block are both arranged in the cylindrical structure; the limiting sliding block is rotatably connected with the transfer rod sliding rail, the transmission rod penetrates through the limiting sliding block and is rotatably connected with the moving sliding block, and the transmission rod is fixedly connected with the limiting sliding block; a track connecting part is arranged on the side part of the movable sliding block, and a track clamping part matched with the track connecting part is arranged on the inner wall of the transfer rod sliding rail; a rotary clamping part is arranged on one side, away from the movable sliding block, of the limiting sliding block; the movable sliding block is provided with a screw rod transmission part matched with the transmission screw rod;
the rotating device comprises a second power device and a second transmission assembly, and the second transmission assembly is arranged at the power output end of the second power device; the second transmission assembly is provided with a rotary butt joint part matched with the rotary clamping part; the transmission rod penetrates through the rotary butt joint part;
in a first working state, the movable sliding block, the limiting sliding block and the transmission rod form a movable assembly; in a second working state, the limiting slide block is fixedly clamped with the second transmission assembly, and the transmission rod, the limiting slide block and the second transmission assembly form a rotating assembly;
in a first working state, the moving assembly is driven by the first power device to move to a first preset position along the longitudinal direction of the transmission rod, the first preset position is that one end, far away from the first power device, of the transmission rod is in butt joint with the transfer device, and the limiting slide block is clamped and fixed with the second transmission assembly; in a second working state, the rotating assembly is driven by the second power device to rotate to a second preset position, and the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device; in a third working state, the transfer device provided with the sample moves away from the chamber of the sample preparation device to the vacuum docking mechanism under the driving of the first power device.
2. The system for transferring a cryo-electron microscope sample according to claim 1, wherein the transmission rod comprises a transmission rod body, a first end and a second end, the first end is used for being connected with the base of the transfer table in a clamping manner, and the second end is used for being rotatably connected with the movable slide block;
the first end comprises a first limiting section, a clamping section and a second limiting section, and the clamping section is arranged between the first limiting section and the second limiting section; the outer diameter of the first limiting section is D1, the outer diameter of the clamping section is D2, and the outer diameter of the second limiting section is D3; d1 > D2, and D3 > D2;
the clamping section comprises an arc area and a plane area, the arc area comprises a first arc portion and a second arc portion which are oppositely arranged, the plane area comprises a first plane portion and a second plane portion which are oppositely arranged, and the first arc portion is arranged between the first plane portion and the second plane portion.
3. The system for transferring a cryo-electron microscope sample according to claim 2, wherein the rail connection portion comprises a movement limiting portion, a first rail engaging portion, a second rail engaging portion;
the rail clamping part comprises a rail, a first clamping bulge and a second clamping bulge which are respectively matched with the movement limiting part, the first slide rail clamping part and the second slide rail clamping part;
the moving limiting part is a T-shaped bulge, and the track is a T-shaped groove;
the first slide rail clamping part is a first groove;
the second slide rail clamping part is a second groove;
the longitudinal axis of the T-shaped groove, the longitudinal axis of the first clamping protrusion and the longitudinal axis of the second clamping protrusion are parallel to the longitudinal axis of the transmission rod.
4. The system for transferring a cryo-electron microscope sample according to claim 3, wherein the screw transmission is a threaded through hole;
one side of the movable sliding block, which is far away from the first power device, is also provided with an elastic piece connecting part and a transmission rod connecting part which is rotatably connected with the second end;
a rotary groove is formed in one side, away from the second power device, of the limiting sliding block, an elastic connecting piece is arranged in the rotary groove, and one end, away from the rotary groove, of the elastic connecting piece is fixedly connected with the elastic piece connecting part; one end of the elastic connecting piece, which is far away from the movable sliding block, is connected with the rotating groove in a sliding manner;
the rotary groove is a kidney-shaped groove, and the maximum angle of the elastic connecting piece rotating in the kidney-shaped groove is
The rotation angle of the transmission rod from the first working state to the second working state is
，
。
5. The system for transferring a cryo-electron microscope sample according to claim 4, wherein the first transmission assembly comprises a first transmission gear and a second transmission gear, the first transmission gear is fixedly arranged on a power output shaft of the first power device, and the second transmission gear is in meshing transmission with the first transmission gear; one end of the transmission screw rod is fixedly connected with the second transmission gear;
the second transmission assembly comprises a third transmission gear and a fourth transmission gear, the third transmission gear is fixedly arranged on a power output shaft of the second power device, and the fourth transmission gear is in meshing transmission with the third transmission gear;
the rotary butt joint part is arranged on one side of the fourth transmission gear facing the first power device;
the rotating device is arranged on the accommodating shell, and the accommodating shell is arranged on the outer side of the vacuum butt joint mechanism.
6. The system for transferring a cryo-electron microscope sample according to claim 5, wherein the vacuum docking mechanism comprises a docking fixing and clamping mechanism and a transfer box housing, the docking fixing and clamping mechanism being configured to be fixedly connected to an outer wall of the sample preparation device;
the transfer box shell is arranged between the butt joint fixing clamping mechanism and the accommodating shell; the transfer box shell is provided with a first through hole and a second through hole for the transmission rod to penetrate through, a vacuum gate valve is arranged between the first through hole and the butt joint fixing clamping mechanism to control the connection and disconnection between the interior of the transfer box shell and the butt joint fixing clamping mechanism, and the vacuum gate valve is in signal connection with the master control center; a magnetic fluid sealing bearing is arranged between the second through hole and the accommodating shell;
a vacuum pump opening and closing port is formed in the side part of the butt joint fixing and clamping mechanism;
the transfer box comprises a transfer box shell, a heat insulation base and a heat insulation base, wherein the transfer box shell is internally provided with a hollow cavity, and the heat insulation base is arranged on the inner wall of the hollow cavity so as to keep the temperature inside the hollow cavity; a liquid nitrogen storage tank is arranged inside the heat preservation base so as to adjust the internal temperature of the hollow cavity;
a fixed base is further arranged inside the hollow cavity to limit the transfer device for bearing the sample; the fixed base and the transfer table base are arranged in the same structure.
7. The system for transferring a cryo-electron microscope sample according to claim 6, wherein the distance from the connecting portion of the transmission screw and the second transmission gear to the outer wall of the transfer box housing is greater than the length of the transmission screw.
8. A method for transferring a sample for a cryo-electron microscope, which is based on the system for transferring a cryo-electron microscope sample as claimed in any one of claims 1 to 7, comprising the following steps:
step S100, opening a vacuum gate valve arranged in a vacuum butt joint mechanism, starting a first power device, and moving a moving assembly to a first preset position along the longitudinal direction of a transmission rod under the driving of the first power device; the first preset position is that one end of the transmission rod, which is far away from the first power device, is butted with the transfer device, and the limiting slide block is clamped and fixed with the second transmission assembly;
step S200, starting a second power device, and rotating the rotating assembly to a second preset position under the driving of the second power device; the second preset position is that one end of the transmission rod, which is far away from the first power device, is clamped and fixed with the transfer device;
step S300, starting a first power device, and moving the transfer device provided with the sample far away from the chamber of the sample preparation device into the chamber of the vacuum docking mechanism under the driving of the first power device;
s400, closing a vacuum gate valve arranged in the vacuum butt joint mechanism, and vacuumizing the cavity of the vacuum butt joint mechanism to enable the sample to be in a vacuum environment;
step S500, a locking switch of a butt joint fixing clamping mechanism in the vacuum butt joint mechanism is controlled, so that the vacuum butt joint mechanism is separated from the outer side of the cavity of the sample preparation device.
9. An electronic device, comprising: at least one processor; and a memory communicatively coupled to at least one of the processors; wherein the memory stores instructions executable by the processor for execution by the processor to implement the cryo-electron microscopy sample transfer method of claim 8.
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