CN113092747B - Light source component for detecting luminous immunity analyzer - Google Patents
Light source component for detecting luminous immunity analyzer Download PDFInfo
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- CN113092747B CN113092747B CN202110401552.3A CN202110401552A CN113092747B CN 113092747 B CN113092747 B CN 113092747B CN 202110401552 A CN202110401552 A CN 202110401552A CN 113092747 B CN113092747 B CN 113092747B
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- light
- light source
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- connecting piece
- detection
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- 230000036039 immunity Effects 0.000 title description 2
- 238000003018 immunoassay Methods 0.000 claims abstract description 25
- 230000005540 biological transmission Effects 0.000 claims abstract description 13
- 238000004020 luminiscence type Methods 0.000 claims description 27
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 21
- 229910052744 lithium Inorganic materials 0.000 claims description 21
- 238000001514 detection method Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 6
- 230000002238 attenuated effect Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000013307 optical fiber Substances 0.000 claims description 3
- 239000000891 luminescent agent Substances 0.000 abstract description 8
- 238000004321 preservation Methods 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 description 14
- ZMHWQAHZKUPENF-UHFFFAOYSA-N 1,2-dichloro-3-(4-chlorophenyl)benzene Chemical compound C1=CC(Cl)=CC=C1C1=CC=CC(Cl)=C1Cl ZMHWQAHZKUPENF-UHFFFAOYSA-N 0.000 description 4
- 238000013016 damping Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- XECAHXYUAAWDEL-UHFFFAOYSA-N acrylonitrile butadiene styrene Chemical compound C=CC=C.C=CC#N.C=CC1=CC=CC=C1 XECAHXYUAAWDEL-UHFFFAOYSA-N 0.000 description 1
- 239000004676 acrylonitrile butadiene styrene Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B45/00—Circuit arrangements for operating light-emitting diodes [LED]
- H05B45/30—Driver circuits
- H05B45/345—Current stabilisation; Maintaining constant current
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a light source component for detecting a luminous immunoassay analyzer, which comprises a shell main body, a constant light source, an attenuation sheet and a dense light connecting piece, wherein a light transmission channel is arranged in the shell main body, a light ray emission port is arranged on the dense light connecting piece, the constant light source, the attenuation sheet and the light ray emission port are sequentially arranged along the light ray transmission direction of the constant light source, and the dense light connecting piece can be connected with the luminous immunoassay analyzer to be calibrated. The invention uses the constant light source to obtain the repeatedly used weak constant light source through attenuation, thereby avoiding the difference of photon numbers generated by the luminescent agents caused by different batches of the luminescent agents, different manufacturers, different use environments and different operators; the luminescent agent has harsh preservation conditions and is inconvenient to use at the clients; high cost of the luminescent agent, etc.
Description
Technical Field
The invention belongs to the technical field of detection instruments of luminescence immunoassay analyzers, and particularly relates to a light source component for detection of a luminescence immunoassay analyzer.
Background
Currently, manufacturers or research and development of luminescence immunoassay analyzers use a luminescence agent method to calibrate and judge the analyzer, and the working principle is that the luminescence agent is used as a light source, and the luminescence immunoassay analyzer reads the photon number. The difference of photon numbers generated by the luminous agents directly influences the calibration and judgment of the luminous analyzer due to the fact that the luminous agents are different in batches, manufacturers and use environments and operators; the storage condition of the luminescent agent is very harsh, so that the luminescent agent is inconvenient to use at a customer site; the cost of the luminescent agent is generally high, and the luminescent agent is a disposable product, which is unfavorable for saving the cost.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide the light source component for detecting the luminescence immunoassay analyzer, the weak constant light source which is repeatedly used can be obtained through attenuation by using the constant light source, and the problems that the luminescence agent has different photon numbers, severe preservation conditions, inconvenience in use at a customer, high luminescence agent cost and the like caused by different batches of luminescence agents, different manufacturers and different use environments and different operating staff are avoided.
In order to solve the problems, the invention provides a light source component for detecting a luminous immunoassay analyzer, which comprises a shell main body, a constant light source, an attenuation sheet and a dense light connecting piece, wherein a light transmission channel communicated with the dense light connecting piece is arranged in the shell main body, a light ray emission port is arranged on the dense light connecting piece, the constant light source, the attenuation sheet and the light ray emission port are sequentially arranged along the light ray transmission direction of the constant light source, and the dense light connecting piece can be connected with the luminous immunoassay analyzer to be calibrated.
Preferably, the constant light source comprises an LED lamp and a PCB board electrically connected with the LED lamp, and a constant current driving circuit is constructed on the PCB board so as to keep the illumination intensity of the LED lamp stable.
Preferably, the constant light source further comprises a lithium battery, the PCB board is electrically connected with the lithium battery, and the constant current driving circuit is capable of boosting the voltage of the lithium battery to a first voltage and disconnecting the power supply to the LED lamp when the voltage of the lithium battery is lower than a second voltage, wherein the second voltage is lower than the first voltage.
Preferably, a containing cavity is further formed in the shell main body, the light propagation channel is communicated with the containing cavity, and the PCB and the lithium battery are located in the containing cavity.
Preferably, a light-sealing pressing strip is arranged at the through connection part of the light transmission channel and the accommodating cavity, and the LED lamp is arranged on one side of the light-sealing pressing strip, which faces the attenuation sheet.
Preferably, a spring is arranged between the attenuation sheet and the dense light pressing strip, a groove is arranged in the region, corresponding to the light transmission channel, of the dense light connecting piece, and the attenuation sheet is pressed against the bottom wall of the groove by the spring.
Preferably, a power key is arranged on the outer side of the shell main body, and the power key is used for controlling the on-off of the constant light source.
Preferably, the shell main body consists of a box body and a cover plate which are mutually buckled.
Preferably, the material of the dense optical connection piece is an opaque material.
Preferably, an optical fiber is arranged at the light emitting port so as to conduct the light emitted by the constant light source after being attenuated by the attenuation sheet.
According to the light source component for detecting the luminescence immunoassay instrument, the constant light source is used for guaranteeing the constancy of the photon number provided by the light source, the attenuation sheet is used for attenuating the constant photon number to form different photon numbers for detecting, photons in the linear range of the luminescence immunoassay instrument are obtained, and the problems that the photon numbers generated by the luminescence agent are different due to the fact that the luminescence agent is different in batches, different in manufacturers and using environments, different in operating staff, harsh in preservation condition of the luminescence agent, inconvenient to use at a customer, high in cost of the luminescence agent and the like are avoided.
Drawings
FIG. 1 is a schematic perspective view of a light source assembly for detecting a luminescence immunoassay device according to an embodiment of the present invention;
FIG. 2 is an exploded view of the structure of FIG. 1;
fig. 3 is a schematic view of the internal structure of fig. 1.
The reference numerals are expressed as:
1. A housing main body; 11. a receiving chamber; 13. a case body; 14. a cover plate; 2. a constant light source; 21. an LED lamp; 22. a PCB board; 23. a lithium battery; 3. an attenuation sheet; 4. a dense light connection; 41. a light ray emission port; 5. a light-tight pressing strip; 6. and (3) a spring.
Detailed Description
Referring to fig. 1 to 3 in combination, according to an embodiment of the present invention, a light source assembly for detecting a luminescence immunoassay device is provided, which includes a housing main body 1, a constant light source 2, an attenuation sheet 3, and a dense light connector 4, wherein the housing main body 1 has a light propagation channel communicating with the dense light connector 4, the dense light connector 4 has a light emission port 41, and the constant light source 2, the attenuation sheet 3, and the light emission port 41 are sequentially disposed along a light transmission direction of the constant light source 2, and the dense light connector 4 can be connected to a luminescence immunoassay device to be calibrated. In this technical scheme, through the invariable light source 2 guarantees that its photon number (namely photon quantity) that provides is invariable, carries out the different photon numbers that decay formation detection to invariable photon number through the attenuator 3 obtains the photon in the luminous immunoassay appearance linear range, avoids the different, producer is different, the service environment is different, the luminous agent that the operating personnel is different causes produces photon number different, luminous agent storage condition harsh, be inconvenient for use in customer department, luminous agent cost high scheduling problem.
As a specific implementation manner of the constant light source 2, preferably, the constant light source 2 includes an LED lamp 21 and a PCB board 22 electrically connected to the LED lamp 21, and a constant current driving circuit is configured on the PCB board 22, so as to maintain the illumination intensity of the LED lamp 21 stable. It will be appreciated that the LED lamp 21 forms a constant light source by means of a PCB board 22 with a corresponding constant current driving circuit, which is of a strong light type, while it is matched with the attenuator 3 by selecting different light transmittances to form weak light (invisible to naked eyes) of different intensities. Thereby, the attenuation sheet 3 is preferably detachably provided in the light propagation path.
Further, the constant light source 2 further includes a lithium battery 23, the PCB 22 is electrically connected to the lithium battery 23, and the constant current driving circuit can boost the voltage of the lithium battery 23 to a first voltage, and disconnect the power supply to the LED lamp 21 when the voltage of the lithium battery 23 is lower than a second voltage, where the second voltage is lower than the first voltage. More specifically, the LED lamp 21 is powered by the lithium battery 23, and the light intensity of the LED lamp 21 is controlled by the driving chip, and the charging part, the undervoltage indication part and the undervoltage protection part are designed in the circuit, so that the light source has only two states, namely constant light emission or no light emission. The two states may be selected by setting a power key (not shown) on the outer side of the housing body 1, for example, the power key is used to control the on/off of the constant light source 2. As a specific embodiment, the PCB 22 is configured with a lithium battery charging circuit and an LED lamp constant current driving circuit, and a TP4056 chip is used to provide a charging current of 100mA for the lithium battery. The constant current driving of the LED lamp adopts a boosting chip to boost the power supply of the lithium battery to a stable 5V power supply, and then adopts a driving chip to provide constant current for the LED lamp 21 to light the LED lamp, and if the voltage of the battery is lower than 2.7V, the LED lamp is directly disconnected.
In some embodiments, the housing main body 1 is further provided with a containing cavity 11, the light propagation channel is communicated with the containing cavity 11, and the PCB 22 and the lithium battery 23 are located in the containing cavity 11, so that the arrangement of the PCB 22, the lithium battery 23 and the LED lamp 21 in the housing main body 1 can be more reasonable.
Preferably, a light-sealing strip 5 (light-proof material) is arranged at the through connection part of the light transmission channel and the accommodating cavity 11, and the LED lamp 21 is arranged on one side of the light-sealing strip 5 facing the attenuation sheet 3, so that the interference of external light on the LED lamp 21 is effectively avoided.
In some embodiments, a spring 6 is sandwiched between the attenuation sheet 3 and the light-tight pressing strip 5, a groove (not labeled in the figure) is provided in a region corresponding to the light propagation channel on the light-tight connector 4, and the attenuation sheet 3 is pressed against a bottom wall of the groove by the spring 6. The spring 6 can fix the damping piece 3, so that the damping piece 3 is in flexible contact with the groove (pressure is generated by deformation of the spring), the contact reliability between the damping piece 3 and the dense optical connecting piece 4 can be ensured, and the damping piece 3 is not damaged.
The shell main body 1 is composed of a box body 13 and a cover plate 14 which are mutually buckled, so that components in the shell main body can be assembled and maintained more conveniently. The material of the dense optical connector 4 is one of opaque materials such as aluminum, ABS, iron, steel, SP and the like.
The light emitting port 41 is provided with an optical fiber, so as to conduct the light emitted by the constant light source 2 after being attenuated by the attenuation sheet 3, and ensure the transmission quality of the light.
It will be readily appreciated by those skilled in the art that the above advantageous ways can be freely combined and superimposed without conflict.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention. The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that modifications and variations can be made without departing from the technical principles of the present invention, and these modifications and variations should also be regarded as the scope of the invention.
Claims (9)
1. The light source component for detecting the luminous immunoassay analyzer is characterized by comprising a shell main body (1), a constant light source (2), an attenuation sheet (3) and a dense light connecting piece (4), wherein a light transmission channel communicated with the dense light connecting piece (4) is arranged in the shell main body (1), a light emission port (41) is formed in the dense light connecting piece (4), the constant light source (2), the attenuation sheet (3) and the light emission port (41) are sequentially arranged along the light transmission direction of the constant light source (2), and the dense light connecting piece (4) can be connected with the luminous immunoassay analyzer to be calibrated; a containing cavity (11) is also formed in the shell main body (1), and the light transmission channel and the containing cavity (11) are communicated; the constant light source (2) comprises an LED lamp (21) and a PCB (22) electrically connected with the LED lamp (21), a light transmission channel and a through connection part of the accommodating cavity (11) are provided with a light sealing strip (5), and the LED lamp (21) is arranged on one side of the light sealing strip (5) facing the attenuation sheet (3).
2. The light source assembly for detection of a luminescence immunoassay instrument according to claim 1, wherein a constant current driving circuit is configured on the PCB board (22) to keep the illumination intensity of the LED lamp (21) stable.
3. The light source assembly for detection of a luminescence immunoassay instrument according to claim 2, wherein the constant light source (2) further comprises a lithium battery (23), the PCB board (22) and the lithium battery (23)
And the constant current driving circuit can boost the voltage of the lithium battery (23) to a first voltage, and disconnect the power supply to the LED lamp (21) when the voltage of the lithium battery (23) is lower than a second voltage, wherein the second voltage is lower than the first voltage.
4. A light source assembly for detection of a luminescence immunoassay instrument according to claim 3, characterized in that the PCB board (22) and the lithium battery (23) are located in the housing cavity (11).
5. The light source assembly for detection of a luminescence immunoassay instrument according to claim 1, wherein a spring (6) is sandwiched between the attenuation sheet (3) and the light-tight pressing strip (5), a groove is provided in a region of the light-tight connecting piece (4) corresponding to the light propagation channel, and the attenuation sheet (3) is pressed against a bottom wall of the groove by the spring (6).
6. The light source assembly for detection of a luminescence immunoassay instrument according to claim 1, wherein a power key is provided on the outer side of the housing main body (1), and the power key is used for controlling on-off of the constant light source (2).
7. The light source assembly for detection of a luminescence immunoassay instrument according to claim 1, wherein the housing main body (1) is composed of a case body (13) and a cover plate (14) which are fastened to each other.
8. The light source assembly for detection of a luminescence immunoassay instrument according to claim 1, wherein the material of the light-tight connector (4) is a light-tight material.
9. The light source assembly for detection of a luminescence immunoassay instrument according to claim 1, wherein an optical fiber is provided at the light emitting port (41) to conduct the light emitted from the constant light source (2) after being attenuated by the attenuation sheet (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110401552.3A CN113092747B (en) | 2021-04-14 | 2021-04-14 | Light source component for detecting luminous immunity analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110401552.3A CN113092747B (en) | 2021-04-14 | 2021-04-14 | Light source component for detecting luminous immunity analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113092747A CN113092747A (en) | 2021-07-09 |
| CN113092747B true CN113092747B (en) | 2024-05-31 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110401552.3A Active CN113092747B (en) | 2021-04-14 | 2021-04-14 | Light source component for detecting luminous immunity analyzer |
Country Status (1)
| Country | Link |
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| CN (1) | CN113092747B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201875447U (en) * | 2010-06-12 | 2011-06-22 | 北京众合百克科学仪器技术有限公司 | Standard light source for chemical illumination immunity analysis instrument |
| DE202015100937U1 (en) * | 2014-02-28 | 2015-06-08 | Skannex As | Portable immunochromatographic analyzer |
| CN207366448U (en) * | 2017-08-24 | 2018-05-15 | 北京贝泰科技有限公司 | A kind of instant detecting system of light-induced chemiluminescent |
| CN109142333A (en) * | 2018-08-16 | 2019-01-04 | 北京乐普医疗科技有限责任公司 | A kind of detection system to fail for reflector on chemical illumination immunity analysis instrument |
| CN208399816U (en) * | 2018-08-02 | 2019-01-18 | 中国人民解放军陆军工程大学 | Wide-range high-precision continuous laser attenuation device |
| CN110927136A (en) * | 2019-12-25 | 2020-03-27 | 杭州微策生物技术有限公司 | Quality detection device of dry-type immunofluorescence POCT detection instrument and use method thereof |
| CN111373267A (en) * | 2018-01-26 | 2020-07-03 | 株式会社日立高新技术 | Automatic analyzer and method for controlling automatic analyzer |
| CN111650170A (en) * | 2020-07-03 | 2020-09-11 | 中国计量科学研究院 | Measurement standard for calibration of luminescence immunoassay analyzer for bottom or lateral detection |
| CN214669105U (en) * | 2021-04-14 | 2021-11-09 | 成都爱兴生物科技有限公司 | Light source device for calibrating luminous immunity analyzer |
-
2021
- 2021-04-14 CN CN202110401552.3A patent/CN113092747B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201875447U (en) * | 2010-06-12 | 2011-06-22 | 北京众合百克科学仪器技术有限公司 | Standard light source for chemical illumination immunity analysis instrument |
| DE202015100937U1 (en) * | 2014-02-28 | 2015-06-08 | Skannex As | Portable immunochromatographic analyzer |
| CN207366448U (en) * | 2017-08-24 | 2018-05-15 | 北京贝泰科技有限公司 | A kind of instant detecting system of light-induced chemiluminescent |
| CN111373267A (en) * | 2018-01-26 | 2020-07-03 | 株式会社日立高新技术 | Automatic analyzer and method for controlling automatic analyzer |
| CN208399816U (en) * | 2018-08-02 | 2019-01-18 | 中国人民解放军陆军工程大学 | Wide-range high-precision continuous laser attenuation device |
| CN109142333A (en) * | 2018-08-16 | 2019-01-04 | 北京乐普医疗科技有限责任公司 | A kind of detection system to fail for reflector on chemical illumination immunity analysis instrument |
| CN110927136A (en) * | 2019-12-25 | 2020-03-27 | 杭州微策生物技术有限公司 | Quality detection device of dry-type immunofluorescence POCT detection instrument and use method thereof |
| CN111650170A (en) * | 2020-07-03 | 2020-09-11 | 中国计量科学研究院 | Measurement standard for calibration of luminescence immunoassay analyzer for bottom or lateral detection |
| CN214669105U (en) * | 2021-04-14 | 2021-11-09 | 成都爱兴生物科技有限公司 | Light source device for calibrating luminous immunity analyzer |
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| CN113092747A (en) | 2021-07-09 |
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