CN113092747A - Light source component for detection of luminescence immunoassay analyzer - Google Patents
Light source component for detection of luminescence immunoassay analyzer Download PDFInfo
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- CN113092747A CN113092747A CN202110401552.3A CN202110401552A CN113092747A CN 113092747 A CN113092747 A CN 113092747A CN 202110401552 A CN202110401552 A CN 202110401552A CN 113092747 A CN113092747 A CN 113092747A
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- light
- light source
- constant
- luminescence immunoassay
- detection
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 29
- 238000004020 luminiscence type Methods 0.000 title claims abstract description 28
- 238000001514 detection method Methods 0.000 title claims abstract description 17
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 21
- 229910052744 lithium Inorganic materials 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 6
- 230000002238 attenuated effect Effects 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 3
- 239000013307 optical fiber Substances 0.000 claims description 3
- 239000000891 luminescent agent Substances 0.000 abstract description 22
- 239000003795 chemical substances by application Substances 0.000 abstract 3
- ZMHWQAHZKUPENF-UHFFFAOYSA-N 1,2-dichloro-3-(4-chlorophenyl)benzene Chemical compound C1=CC(Cl)=CC=C1C1=CC=CC(Cl)=C1Cl ZMHWQAHZKUPENF-UHFFFAOYSA-N 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- XECAHXYUAAWDEL-UHFFFAOYSA-N acrylonitrile butadiene styrene Chemical compound C=CC=C.C=CC#N.C=CC1=CC=CC=C1 XECAHXYUAAWDEL-UHFFFAOYSA-N 0.000 description 1
- 239000004676 acrylonitrile butadiene styrene Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B45/00—Circuit arrangements for operating light-emitting diodes [LED]
- H05B45/30—Driver circuits
- H05B45/345—Current stabilisation; Maintaining constant current
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a light source component for detection of a luminescence immunoassay analyzer, which comprises a shell main body, a constant light source, an attenuation sheet and a light-tight connecting piece, wherein a light propagation channel is arranged in the shell main body, a light emitting outlet is arranged on the light-tight connecting piece, the constant light source, the attenuation sheet and the light emitting outlet are sequentially arranged along the light conducting direction of the constant light source, and the light-tight connecting piece can be connected with the luminescence immunoassay analyzer to be calibrated. The invention can obtain the repeatedly used weak constant light source by using the constant light source through attenuation, thereby avoiding the difference of the number of photons generated by the luminous agent caused by different batches of the luminous agent, different manufacturers, different use environments and different operators; the storage condition of the luminescent agent is harsh, so that the luminescent agent is inconvenient to use at a client; the cost of the luminous agent is high.
Description
Technical Field
The invention belongs to the technical field of detection instruments of a luminescence immunoassay analyzer, and particularly relates to a light source component for detection of the luminescence immunoassay analyzer.
Background
At present, manufacturers or research and development of luminescence immunoassay analyzers use a luminescent agent method to calibrate and judge the analyzers, and the working principle of the luminescence immunoassay analyzer is that a luminescent agent is used as a light source and the luminescence immunoassay analyzer reads photon numbers. The number of photons generated by the luminescent agent is different due to different batches of the luminescent agent, different manufacturers, different use environments and different operators, and the difference of the number of generated photons directly influences the calibration and judgment of the luminescence analyzer; the storage condition of the luminescent agent is very harsh, so that the luminescent agent is inconvenient to use at a client; the cost of the luminescent agent is generally higher, and the luminescent agent is a disposable product, which is not beneficial to saving the cost.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to provide a light source assembly for luminescence immunoassay analyzer detection, which can obtain a repeatedly used weak constant light source by using a constant light source through attenuation, and avoid the problems of different batches of luminescent agents, different manufacturers, different use environments, different operators, different numbers of photons generated by the luminescent agents, harsh storage conditions of the luminescent agents, inconvenience for use at customers, high cost of the luminescent agents, and the like.
In order to solve the above problems, the present invention provides a light source assembly for detection of a luminescence immunoassay analyzer, comprising a housing main body, a constant light source, an attenuation sheet, and a light-tight connector, wherein the housing main body has a light propagation channel communicated with the light-tight connector, the light-tight connector has a light exit port, the constant light source, the attenuation sheet, and the light exit port are sequentially arranged along a light conduction direction of the constant light source, and the light-tight connector can be connected with a luminescence immunoassay analyzer to be calibrated.
Preferably, the constant light source comprises an LED lamp and a PCB electrically connected to the LED lamp, and a constant current driving circuit is configured on the PCB to stabilize the illumination intensity of the LED lamp.
Preferably, the constant light source further comprises a lithium battery, the PCB board is electrically connected to the lithium battery, and the constant current driving circuit is capable of boosting the voltage of the lithium battery to a first voltage and disconnecting power supply to the LED lamp when the voltage of the lithium battery is lower than a second voltage, wherein the second voltage is lower than the first voltage.
Preferably, an accommodating cavity is further configured in the housing main body, the light propagation channel is communicated with the accommodating cavity, and the PCB and the lithium battery are located in the accommodating cavity.
Preferably, a light-tight pressing strip is arranged at the through connection position of the light propagation channel and the accommodating cavity, and the LED lamp is arranged on one side, facing the attenuation sheet, of the light-tight pressing strip.
Preferably, a spring is clamped between the attenuation sheet and the light-tight pressure strip, a groove is formed in an area, corresponding to the light propagation channel, of the light-tight connecting piece, and the attenuation sheet is pressed against the bottom wall of the groove by the spring.
Preferably, a power key is arranged on the outer side of the shell main body and used for controlling the on-off of the constant light source.
Preferably, the housing main body is composed of a box body and a cover plate which are buckled with each other.
Preferably, the material of the light-tight connecting piece is a light-tight material.
Preferably, an optical fiber is arranged at the light exit hole to conduct the light emitted by the constant light source after being attenuated by the attenuation sheet.
According to the light source assembly for the luminescence immunoassay analyzer, the constant number of photons provided by the constant light source is ensured, the constant number of photons is attenuated by the attenuation sheet to form different numbers of photons for detection, photons within the linear range of the luminescence immunoassay analyzer are obtained, and the problems that the number of photons generated by the luminescent agent is different, the storage condition of the luminescent agent is harsh, the use is inconvenient for customers, the cost of the luminescent agent is high and the like caused by different batches of the luminescent agent, different manufacturers, different use environments and different operators are solved.
Drawings
FIG. 1 is a schematic perspective view of a detection light assembly of a luminescence immunoassay analyzer according to an embodiment of the present invention;
FIG. 2 is an exploded view of the structure of FIG. 1;
fig. 3 is a schematic view of the internal structure of fig. 1.
The reference numerals are represented as:
1. a housing main body; 11. an accommodating chamber; 13. a box body; 14. a cover plate; 2. a constant light source; 21. an LED lamp; 22. a PCB board; 23. a lithium battery; 3. an attenuation sheet; 4. a light-tight connector; 41. a light emitting opening; 5. a light-tight batten; 6. a spring.
Detailed Description
Referring to fig. 1 to 3 in combination, according to an embodiment of the present invention, there is provided a light source assembly for detection of a luminescence immunoassay analyzer, including a housing body 1, a constant light source 2, an attenuation sheet 3, and a dense optical connector 4, wherein the housing body 1 has a light propagation channel therein, the dense optical connector 4 has a light exit 41 thereon, the constant light source 2, the attenuation sheet 3, and the light exit 41 are sequentially arranged along a light conduction direction of the constant light source 2, and the dense optical connector 4 can be connected to a luminescence immunoassay analyzer to be calibrated. In the technical scheme, the constant number of photons (namely the photon amount) provided by the constant light source 2 is ensured, the constant number of photons is attenuated by the attenuation sheet 3 to form different photon numbers for detection, photons within the linear range of the luminescence immunoassay analyzer are obtained, and the problems of different photon numbers generated by the luminescent agent, harsh storage conditions of the luminescent agent, inconvenience in use at a client, high cost of the luminescent agent and the like caused by different batches of the luminescent agent, different manufacturers, different use environments and different operators are solved.
As a specific implementation manner of the constant light source 2, it is preferable that the constant light source 2 includes an LED lamp 21 and a PCB 22 electrically connected to the LED lamp 21, and a constant current driving circuit is configured on the PCB 22 to stabilize the illumination intensity of the LED lamp 21. It can be understood that the LED lamp 21 forms a constant light source, which is of a high light type, by the PCB board 22 having a corresponding constant current driving circuit, and forms low light (invisible to naked eyes) of different intensity by selecting different light transmittances to cooperate with the attenuation sheet 3. Thus, the attenuation sheet 3 is preferably detachably provided in the light propagation channel.
Further, the constant light source 2 further includes a lithium battery 23, the PCB 22 is electrically connected to the lithium battery 23, and the constant current driving circuit is capable of boosting the voltage of the lithium battery 23 to a first voltage and disconnecting the power supply to the LED lamp 21 when the voltage of the lithium battery 23 is lower than a second voltage, where the second voltage is lower than the first voltage. More specifically, the LED lamp 21 is powered by the lithium battery 23, the light emitting intensity of the LED lamp 21 is controlled by the driving chip, and the charging portion, the power source under-voltage indication portion and the power source under-voltage protection portion are designed in the circuit, so that the light source has only two states, namely, constant light emitting or no light emitting. The two states can be selected by arranging a power key, for example, a power key (not shown) is arranged on the outer side of the housing main body 1, and the power key is used for controlling the constant light source 2 to be powered on or powered off. As a specific implementation example, the PCB 22 is configured with a lithium battery charging circuit and an LED lamp constant current driving circuit, and a TP4056 chip is used to provide 100mA of charging current for the lithium battery. The constant current driving of the LED lamp adopts a boosting chip to firstly boost a lithium battery power supply to a stable 5V power supply, then adopts a driving chip to provide constant current for the LED lamp 21 to light the LED lamp, and directly cuts off the LED lamp if the voltage of a battery is lower than 2.7V.
In some embodiments, a containing cavity 11 is further configured in the housing main body 1, the light propagation channel is formed to be communicated with the containing cavity 11, and the PCB 22 and the lithium battery 23 are located in the containing cavity 11, so that the arrangement of the PCB 22 and the lithium battery 23 and the LED lamp 21 in the housing main body 1 can be more reasonable.
Preferably, a light-tight pressing strip 5 (made of opaque material) is arranged at a through connection part of the light propagation channel and the accommodating cavity 11, and the LED lamp 21 is installed on one side of the light-tight pressing strip 5 facing the attenuation sheet 3, so that interference of external light on the LED lamp 21 is effectively avoided.
In some embodiments, a spring 6 is interposed between the attenuation sheet 3 and the light-tight pressing strip 5, a groove (not labeled in the figures) is disposed in a region of the light-tight connecting member 4 corresponding to the light propagation channel, and the attenuation sheet 3 is pressed against a groove bottom wall of the groove by the spring 6. The spring 6 can fix the attenuation sheet 3, so that the attenuation sheet 3 is in flexible contact with the groove (pressure is generated by the deformation of the spring), the contact reliability between the attenuation sheet 3 and the dense-contact connector 4 can be ensured, and the attenuation sheet 3 is prevented from being damaged.
The shell body 1 is composed of a box body 13 and a cover plate 14 which are buckled with each other, so that components inside the shell body can be assembled and maintained more conveniently. The material of the dense optical connector 4 is one of opaque materials such as aluminum, ABS, iron, steel, SP and the like.
The light ray outlet 41 is provided with an optical fiber to conduct the light ray emitted by the constant light source 2 after being attenuated by the attenuation sheet 3, so that the transmission quality of the light ray is ensured.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims (10)
1. The utility model provides a luminous light source module for immunoassay appearance detection which characterized in that, includes shell main part (1), constant light source (2), decay piece (3), secret light connecting piece (4), wherein have in the shell main part (1) with the light propagation passageway of secret light connecting piece (4) intercommunication, have light on the secret light connecting piece (4) and go out mouth (41), along the light conduction direction of constant light source (2), decay piece (3), light go out mouth (41) and set gradually, secret light connecting piece (4) can be connected with the luminous immunoassay appearance that treats demarcation.
2. The light source assembly for detection of the luminescence immunoassay analyzer according to claim 1, wherein the constant light source (2) comprises an LED lamp (21) and a PCB board (22) electrically connected with the LED lamp (21), the PCB board (22) is configured with a constant current driving circuit to keep the illumination intensity of the LED lamp (21) stable.
3. The light source module for testing of the luminescence immunoassay analyzer according to claim 2, wherein the constant light source (2) further comprises a lithium battery (23), and the PCB (22) and the lithium battery (23)
And the constant current driving circuit can boost the voltage of the lithium battery (23) to a first voltage and cut off the power supply to the LED lamp (21) when the voltage of the lithium battery (23) is lower than a second voltage, wherein the second voltage is lower than the first voltage.
4. The luminescence immunoassay instrument detection light source assembly according to claim 3, wherein a housing chamber (11) is further configured in the housing main body (1), the light propagation channel is formed to communicate with the housing chamber (11), and the PCB (22) and the lithium battery (23) are disposed in the housing chamber (11).
5. The light source assembly for detection of the luminescence immunoassay analyzer according to claim 4, wherein a light-tight strip (5) is disposed at the through connection of the light propagation channel and the accommodating cavity (11), and the LED lamp (21) is installed on one side of the light-tight strip (5) facing the attenuation sheet (3).
6. The light source assembly for detection of the luminescence immunoassay analyzer according to claim 5, wherein a spring (6) is clamped between the attenuation sheet (3) and the light-tight strip (5), a groove is arranged on the light-tight connecting piece (4) in a region corresponding to the light propagation channel, and the attenuation sheet (3) is pressed against the bottom wall of the groove by the spring (6).
7. The light source module for detection of the luminescence immunoassay analyzer according to claim 1, wherein a power key for controlling the on/off of the constant light source (2) is provided on the outer side of the housing main body (1).
8. The light source module for testing of the luminescence immunoassay analyzer according to claim 1, wherein the housing main body (1) is composed of a box body (13) and a cover plate (14) which are engaged with each other.
9. The light source module for testing of the luminescence immunoassay analyzer according to claim 1, wherein the material of the light-tight connector (4) is opaque.
10. The light source module for testing of a luminescence immunoassay instrument according to claim 1, wherein an optical fiber is provided at the light emitting port (41) to conduct the light emitted from the constant light source (2) after being attenuated by the attenuating sheet (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110401552.3A CN113092747B (en) | 2021-04-14 | Light source component for detecting luminous immunity analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110401552.3A CN113092747B (en) | 2021-04-14 | Light source component for detecting luminous immunity analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113092747A true CN113092747A (en) | 2021-07-09 |
| CN113092747B CN113092747B (en) | 2024-05-31 |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201875447U (en) * | 2010-06-12 | 2011-06-22 | 北京众合百克科学仪器技术有限公司 | Standard light source for chemical illumination immunity analysis instrument |
| DE202015100937U1 (en) * | 2014-02-28 | 2015-06-08 | Skannex As | Portable immunochromatographic analyzer |
| CN207366448U (en) * | 2017-08-24 | 2018-05-15 | 北京贝泰科技有限公司 | A kind of instant detecting system of light-induced chemiluminescent |
| CN109142333A (en) * | 2018-08-16 | 2019-01-04 | 北京乐普医疗科技有限责任公司 | A kind of detection system to fail for reflector on chemical illumination immunity analysis instrument |
| CN208399816U (en) * | 2018-08-02 | 2019-01-18 | 中国人民解放军陆军工程大学 | A kind of wide-range high-precision continuous laser attenuating device |
| CN110927136A (en) * | 2019-12-25 | 2020-03-27 | 杭州微策生物技术有限公司 | Quality detection device of dry-type immunofluorescence POCT detection instrument and use method thereof |
| CN111373267A (en) * | 2018-01-26 | 2020-07-03 | 株式会社日立高新技术 | Automatic analyzer and method for controlling automatic analyzer |
| CN111650170A (en) * | 2020-07-03 | 2020-09-11 | 中国计量科学研究院 | Measurement standard for calibration of luminescence immunoassay analyzer for bottom or lateral detection |
| CN214669105U (en) * | 2021-04-14 | 2021-11-09 | 成都爱兴生物科技有限公司 | Light source device for calibrating luminous immunity analyzer |
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201875447U (en) * | 2010-06-12 | 2011-06-22 | 北京众合百克科学仪器技术有限公司 | Standard light source for chemical illumination immunity analysis instrument |
| DE202015100937U1 (en) * | 2014-02-28 | 2015-06-08 | Skannex As | Portable immunochromatographic analyzer |
| CN207366448U (en) * | 2017-08-24 | 2018-05-15 | 北京贝泰科技有限公司 | A kind of instant detecting system of light-induced chemiluminescent |
| CN111373267A (en) * | 2018-01-26 | 2020-07-03 | 株式会社日立高新技术 | Automatic analyzer and method for controlling automatic analyzer |
| CN208399816U (en) * | 2018-08-02 | 2019-01-18 | 中国人民解放军陆军工程大学 | A kind of wide-range high-precision continuous laser attenuating device |
| CN109142333A (en) * | 2018-08-16 | 2019-01-04 | 北京乐普医疗科技有限责任公司 | A kind of detection system to fail for reflector on chemical illumination immunity analysis instrument |
| CN110927136A (en) * | 2019-12-25 | 2020-03-27 | 杭州微策生物技术有限公司 | Quality detection device of dry-type immunofluorescence POCT detection instrument and use method thereof |
| CN111650170A (en) * | 2020-07-03 | 2020-09-11 | 中国计量科学研究院 | Measurement standard for calibration of luminescence immunoassay analyzer for bottom or lateral detection |
| CN214669105U (en) * | 2021-04-14 | 2021-11-09 | 成都爱兴生物科技有限公司 | Light source device for calibrating luminous immunity analyzer |
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