CN113092730A - Test device for microorganism induced calcium carbonate deposition - Google Patents
Test device for microorganism induced calcium carbonate deposition Download PDFInfo
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- CN113092730A CN113092730A CN202110408577.6A CN202110408577A CN113092730A CN 113092730 A CN113092730 A CN 113092730A CN 202110408577 A CN202110408577 A CN 202110408577A CN 113092730 A CN113092730 A CN 113092730A
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Abstract
The invention discloses a test device for microorganism-induced calcium carbonate deposition, which relates to the technical field of test devices and comprises the following components: the test stand is provided with a first support plate and a second support plate which are arranged at intervals up and down, and the first support plate and the second support plate are respectively provided with a plurality of placing through holes; annotate the liquid subassembly and include: the liquid storage bottle is detachably arranged in the corresponding placing through hole of the first supporting plate; the pipe is placed to the through-hole that places that the second backup pad corresponds in being equipped with and being used for holding the sand test piece of treating bonding and all being equipped with the open-ended test piece from top to bottom, the internal diameter of catheter is less than the internal diameter that the pipe was placed to the test piece and the center that the pipe was placed to the lower extreme of catheter and test piece corresponds the setting, be equipped with governing valve and rotor flow meter on the catheter, the device simple structure, convenient operation uses the device can effectively improve the induced calcium carbonate deposit test piece self intensity of microorganism and grout efficiency.
Description
Technical Field
The invention relates to the technical field of test devices, in particular to a test device for microorganism-induced calcium carbonate deposition.
Background
Worldwide, new research opportunities are created in the emerging field of biometric soil improvement due to the need for new and sustainable methods to solve challenging geotechnical engineering problems. Among these processes, the most widely studied is the microbial induction of calcite precipitation.
In the process of performing the cementation test, if the bacterial liquid is injected from the top end to the bottom end of the container, most of the bacterial liquid seeps through the outer wall due to the influence of the wall attachment effect, and the problem of bacterial liquid permeation is more prominent when the cementation test is performed on sandy soil with higher compactness. If the method of injecting the bacteria liquid from the bottom end to the top end of the container is adopted, the problem of the distribution of the bacteria liquid can be solved, but the method is not consistent with the actual experiment. In addition, the bacterial liquid is unevenly distributed due to the fact that the grouting speed cannot be controlled in the grouting process, so that the internal cementing substances are unevenly distributed after mineralization, and finally the cementing effect is poor. In summary, the existing laboratory device for inducing the calcium carbonate deposition by microorganisms still has some defects.
Disclosure of Invention
The invention aims to provide a test device for microbial induction of calcium carbonate deposition, which is simple in structure and convenient to operate, and can effectively improve the strength and grouting efficiency of a microbial induction calcium carbonate deposition test piece.
The technical scheme for solving the technical problems is as follows:
a microbial induced calcium carbonate deposition test device, comprising: the test stand is provided with a first support plate and a second support plate which are arranged at intervals up and down, and the first support plate and the second support plate are respectively provided with a plurality of correspondingly arranged placing through holes; annotate the liquid subassembly and include: the liquid storage bottle is detachably arranged in the corresponding placing through hole of the first supporting plate; the pipe is placed to the lower extreme that is equipped with in the through-hole of placing that the second backup pad corresponds and is used for holding the sand test piece of treating bonding and all is equipped with the open-ended test piece from top to bottom, and the internal diameter of catheter is less than the internal diameter that the pipe was placed to the test piece and the center that the pipe was placed to the lower extreme of catheter and test piece corresponds the setting, is equipped with governing valve and rotor flow meter on the catheter.
The test device is provided with a test frame which has good supporting effect on the whole test device, the test frame is provided with a first supporting plate and a second supporting plate which are arranged at intervals, and the two supporting plates are provided with correspondingly arranged placing through holes, the first supporting plate is used for placing a liquid storage bottle for containing bacterial liquid, the lower end of the liquid storage bottle is provided with a liquid guide pipe, the bacterial liquid in the liquid storage bottle can smoothly enter a test piece placing pipe correspondingly placed on the second supporting plate through the liquid guide pipe, and because the inner diameter of the liquid guide pipe is smaller than the inner diameter of the upper end of the test piece placing pipe, and the lower end of the liquid guide pipe is arranged corresponding to the center of the test piece placing pipe, the bacteria liquid can enter the test piece placing tube along the center of the test piece placing tube to reduce the bacteria liquid flowing to the wall surface, and the liquid guide tube is provided with a regulating valve and a rotor flow meter, therefore, the wall attachment effect when the bacteria liquid is poured into the test piece placing tube can be reduced by adjusting the liquid outlet speed and the liquid outlet quantity.
Further, in a preferred embodiment of the present invention, the catheter is a flexible tube, and a detachable positioning assembly is disposed at the lower end of the catheter, the positioning assembly comprising: the liquid guide short pipe is detachably connected with the liquid guide pipe, the fixing cover is arranged on the liquid guide short pipe and is far away from one end of the liquid guide pipe, the opening of the fixing cover faces downwards, the liquid guide short pipe and the fixing cover are coaxially arranged, and the outer diameter of the fixing cover is matched with the inner diameter of the test piece placing pipe.
The liquid guide pipe adopts a hose, and the liquid outlet of the liquid guide pipe can be aligned to the center of the test piece placing pipe through the positioning assembly; because the drain nozzle stub sets up in the center of fixed cover, the external diameter of fixed cover and the internal diameter phase-match of pipe are placed to the test piece, consequently when fixed cover placed the test piece and place the pipe in, the drain nozzle stub corresponds with the center that the pipe was placed to the test piece promptly. Because the catheter is a hose, the position of the liquid outlet at the lower end of the catheter can be accurately positioned through the positioning assembly, and the catheter is easy to adjust relative to a rigid pipe and meets the requirement of simple operation.
Further, in a preferred embodiment of the present invention, the liquid guiding short tube is a hard tube, one end of the liquid guiding short tube away from the fixing cover is provided with a tip structure, and the liquid guiding tube is sleeved on the outer wall of the liquid guiding short tube.
The short liquid guiding pipe adopts a hard pipe, and the end part of the short liquid guiding pipe is provided with a tip, so that the short liquid guiding pipe can be conveniently inserted into a soft liquid guiding pipe for fixing.
Further, in a preferred embodiment of the present invention, the lower end of the liquid storage bottle is provided with a hard short connection pipe matched with the liquid guide pipe, and the liquid guide pipe is sleeved on the outer wall of the short connection pipe.
The liquid guide pipe is sleeved on the hard short connecting pipe, so that the assembly and the disassembly are convenient.
Further, in a preferred embodiment of the present invention, the outer walls of the upper ends of the liquid storage bottle and the test piece placement tube are provided with bosses, the outer diameters of the bosses are larger than the inner diameter of the placement through hole, and the outer diameters of the liquid storage bottle and the test piece placement tube are smaller than the inner diameter of the placement through hole.
Can place the pipe through the boss with stock solution bottle and test piece and take and put in the through-hole of placing of first backup pad and second backup pad, place easy operation, place stably.
Further, in the preferred embodiment of the present invention, a detachable end cap is disposed at the opening of the upper end of the liquid storage bottle.
The upper end of stock solution bottle sets up the end cover, and the fungus liquid seals through the end cover after holding in the stock solution bottle, avoids impurity to get into the contaminated fungus liquid.
Further, in the preferred embodiment of the present invention, the liquid storage bottle and the test piece placing tube are made of transparent plastic materials.
The liquid storage bottle and the test piece placing pipe are made of transparent plastic materials, cleaning and disinfection are facilitated, and the transparent structure facilitates observation of testers at any time.
Further, in a preferred embodiment of the present invention, the outer wall of the liquid storage bottle is provided with scales.
The scale of stock solution bottle outer wall provides data reference for the fungus liquid volume that holds.
Further, in a preferred embodiment of the present invention, the test rack further comprises: the interval sets up the bottom plate of keeping away from first backup pad one end in the second backup pad, is equipped with the support column of many vertical settings between first backup pad and the second backup pad and between second backup pad and the bottom plate respectively.
Further, in the preferred embodiment of the present invention, the test rack is made of stainless steel.
The invention has the following beneficial effects:
the invention provides a test device for microorganism-induced calcium carbonate deposition, which is provided with a test rack, wherein a plurality of groups of liquid injection assemblies can be arranged on the test rack, a plurality of groups of different tests can be simultaneously carried out, a liquid outlet of a liquid injection pipe in the liquid injection assemblies corresponds to the center of a test piece placing pipe, and an adjusting valve and a rotor flow meter are arranged on the liquid injection pipe, so that the dripping direction, speed and flow of bacteria liquid can be controlled, and the wall attachment effect during bacteria liquid permeation is reduced as much as possible.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a test apparatus for inducing calcium carbonate deposition by microorganisms according to the present invention;
FIG. 2 is a schematic structural view of a priming assembly of the present invention;
fig. 3 is a schematic structural diagram of the positioning assembly of the present invention.
Wherein: 1-a test stand; 11-a first support plate; 111-placing vias; 12-a second support plate; 13-a base plate; 14-a support column; 2-liquid injection assembly; 21-a liquid storage bottle; 211-junction box; 212-a boss; 22-a catheter; 221-regulating valve; 222-a rotameter; 23-test piece placing tube; 24-a positioning assembly; 241-a liquid guiding short pipe; 242-fixed cover; 3-a sandy soil test piece to be bonded.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
Examples
Referring to fig. 1, a microbial induced calcium carbonate deposition assay device, comprising: test frame 1 and detachable set up the multiunit on test frame 1 and annotate liquid subassembly 2, and test frame 1 wholly adopts stainless steel, and first backup pad 11 and second backup pad 12 that the interval set up about being equipped with on test frame 1 still include: the interval sets up the bottom plate 13 of keeping away from 11 one end of first backup pad in second backup pad 12, be equipped with the support column 14 of four vertical settings between first backup pad 11 and the second backup pad 12 and between second backup pad 12 and bottom plate 13 respectively, be equipped with nine through-holes 111 of placing that correspond the setting on first backup pad 11 and the second backup pad 12 respectively, it is the circular port to place through-hole 111, in this embodiment, first backup pad 11, second backup pad 12 and bottom plate 13 all adopt length to be 850mm, the width is 250 mm's stainless steel board, the internal diameter of placing through-hole 111 is 38 mm.
Referring to fig. 1 and 2, the priming assembly 2 includes: liquid storage bottle 21 of upper end open-ended and set up catheter 22 in liquid storage bottle 21 lower extreme, liquid storage bottle 21 adopt transparent plastics material, are equipped with the scale on the outer wall of liquid storage bottle 21, and in this embodiment, the maximum capacity of liquid storage bottle 21 is 60ml, and its internal diameter is 30mm, and the height is 11 cm. The setting of stock solution bottle 21 detachable is in the through-hole 111 of placing that first backup pad 11 corresponds, and stock solution bottle 21 adopts a cylinder structure, is equipped with annular boss 212 of circle on the outer wall of stock solution bottle 21 upper end, and the external diameter of boss 212 is greater than the internal diameter of placing through-hole 111, and the external diameter of stock solution bottle 21 is less than the internal diameter of placing through-hole 111, and the opening part of stock solution bottle 21 upper end still is equipped with the detachable end cover. In the present embodiment, the boss 212 has an outer diameter of 45 mm.
Referring to fig. 1, the corresponding through-hole 111 of placing of second backup pad 12 is equipped with in and is used for holding waiting to bond sand soil test piece 3 and all is equipped with the open-ended test piece from top to bottom and places pipe 23, and the pipe 23 is placed to the test piece also adopts a cylinder structure, and the pipe 23 is placed to the test piece adopts transparent plastics material, and in this embodiment, the structure and the size that pipe 23 was placed to the test piece are the same with the structure and the size of stock solution bottle 21. The outer wall of the upper end of the test piece placing pipe 23 is also provided with a circular boss 212, so that the test piece placing pipe 23 can be conveniently placed in the placing through hole 111, the inner diameter of the liquid guide pipe 22 is smaller than that of the test piece placing pipe 23, the lower end of the liquid guide pipe 22 corresponds to the center of the test piece placing pipe 23, and the liquid guide pipe 22 is provided with an adjusting valve 221 and a rotor flow meter 222. The catheter 22 is a flexible pipe, in this embodiment, the inner diameter of the catheter 22 is 1.6mm, the length is 70cm, the lower end of the catheter 22 is provided with a detachable positioning component 24, and the positioning component 24 includes: the liquid guiding short pipe 241 is detachably connected with the liquid guiding pipe 22, and the fixing cover 242 with an opening facing downwards is arranged at one end, away from the liquid guiding pipe 22, of the liquid guiding short pipe 241, the liquid guiding short pipe 241 and the fixing cover 242 are coaxially arranged, the outer diameter of the fixing cover 242 is matched with the inner diameter of the test piece placing pipe 23, and the fixing cover 242 can be just clamped in the test piece placing pipe 23. The liquid guiding short pipe 241 is a hard pipe, one end of the liquid guiding short pipe 241 far away from the fixing cover 242 is provided with a tip structure, and the liquid guiding pipe 22 is sleeved on the outer wall of the liquid guiding short pipe 241. The lower end of the liquid storage bottle 21 is provided with a hard short connecting pipe 211 matched with the liquid guide pipe 22, and the liquid guide pipe 22 is sleeved on the outer wall of the short connecting pipe 211.
The specific test procedure using the apparatus is described below with reference to fig. 1-3:
assembling the liquid injection assemblies 2 in advance, placing the liquid injection assemblies on a test rack 1, and arranging a plurality of groups of liquid injection assemblies 2 on the test rack according to test requirements; the lower end of the test piece placing pipe 23 is provided with a permeable stone, so that sand samples are prevented from leaking from the bottom when the sand test piece 3 to be bonded is prepared, and then the sand test piece 3 to be bonded is loaded.
During the test, enough bacteria liquid is contained in the liquid storage bottle 21, the regulating valve 221 and the rotor flowmeter 222 are opened, the bacteria liquid is conveyed to the positioning assembly 24 at a constant speed, the bacteria liquid is conveyed to the sandy soil test piece 3 to be bonded through the liquid guide short pipe 241 in the positioning assembly 24 uniformly, the sand soil test piece 3 to be bonded is kept standing for two hours after being filled with the bacteria liquid, the cementing liquid is contained in the liquid storage bottle 21 after the bacteria liquid is completely seeped out, the cementing liquid is filled in the sandy soil test piece 3 to be bonded at a constant speed, a second batch of bacteria liquid is injected after the microorganisms and the mixed liquid completely react, the above processes are repeated until the injected bacteria liquid is not seeped out from the test piece placing pipe 23, and the slurry and.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A test device for microorganism-induced calcium carbonate deposition, comprising: the liquid injection device comprises a test frame (1) and a plurality of groups of liquid injection assemblies (2) which are detachably arranged on the test frame (1), wherein a first supporting plate (11) and a second supporting plate (12) which are arranged at intervals from top to bottom are arranged on the test frame (1), and a plurality of correspondingly arranged placing through holes (111) are respectively formed in the first supporting plate (11) and the second supporting plate (12);
the liquid injection assembly (2) comprises: the liquid storage bottle (21) with an opening at the upper end and a liquid guide pipe (22) arranged at the lower end of the liquid storage bottle (21), wherein the liquid storage bottle (21) is detachably arranged in the placing through hole (111) corresponding to the first supporting plate (11); the placing through hole (111) corresponding to the second supporting plate (12) is internally provided with a test piece placing pipe (23) which is used for containing a sand test piece (3) to be bonded and is provided with openings at the upper end and the lower end, the inner diameter of the liquid guide pipe (22) is smaller than that of the test piece placing pipe (23), the lower end of the liquid guide pipe (22) corresponds to the center of the test piece placing pipe (23), and the liquid guide pipe (22) is provided with a regulating valve (221) and a rotor flow meter (222).
2. The device for testing the microbial-induced calcium carbonate deposition according to claim 1, wherein the liquid guide pipe (22) is a flexible pipe, the lower end of the liquid guide pipe (22) is provided with a detachable positioning assembly (24), and the positioning assembly (24) comprises: the test piece placing tube comprises a liquid guide short tube (241) detachably connected with the liquid guide tube (22) and a fixing cover (242) arranged on the liquid guide short tube (241) and having a downward opening, wherein the end, far away from the liquid guide tube (22), of the liquid guide short tube (241) is provided with a downward opening, the liquid guide short tube (241) and the fixing cover (242) are coaxially arranged, and the outer diameter of the fixing cover (242) is matched with the inner diameter of the test piece placing tube (23).
3. The device for testing the microbial-induced calcium carbonate deposition according to claim 2, wherein the liquid guiding short pipe (241) is a hard pipe, one end of the liquid guiding short pipe (241) far away from the fixing cover (242) is provided with a tip structure, and the liquid guiding pipe (22) is sleeved on the outer wall of the liquid guiding short pipe (241).
4. The device for testing the microbial-induced calcium carbonate deposition according to claim 2, wherein the lower end of the liquid storage bottle (21) is provided with a hard connecting short pipe (211) matched with the liquid guide pipe (22), and the liquid guide pipe (22) is sleeved on the outer wall of the connecting short pipe (211).
5. The device for testing the microbial-induced calcium carbonate deposition according to any one of claims 1 to 4, wherein a boss (212) is arranged on the outer wall of the upper end of the liquid storage bottle (21) and the test piece placing tube (23), the outer diameter of the boss (212) is larger than the inner diameter of the placing through hole (111), and the outer diameter of the liquid storage bottle (21) and the test piece placing tube (23) is smaller than the inner diameter of the placing through hole (111).
6. The device for testing the microbial induction of calcium carbonate deposition according to claim 5, wherein a detachable end cover is arranged at an opening at the upper end of the liquid storage bottle (21).
7. The device for testing the microbial-induced calcium carbonate deposition according to claim 6, wherein the liquid storage bottle (21) and the test piece placing tube (23) are both made of transparent plastic materials.
8. The device for testing the microbial induction of calcium carbonate deposition according to claim 7, wherein the outer wall of the liquid storage bottle (21) is provided with scales.
9. The microbial induced calcium carbonate deposition test device of claim 1, wherein the test rack (1) further comprises: the interval sets up second backup pad (12) are kept away from bottom plate (13) of first backup pad (11) one end, first backup pad (11) with between second backup pad (12) and second backup pad (12) with be equipped with many vertical support columns (14) that set up between bottom plate (13) respectively.
10. The device for testing the microbial induction of calcium carbonate deposition according to claim 9, wherein the test rack (1) is made of stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202110408577.6A CN113092730A (en) | 2021-04-15 | 2021-04-15 | Test device for microorganism induced calcium carbonate deposition |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110408577.6A CN113092730A (en) | 2021-04-15 | 2021-04-15 | Test device for microorganism induced calcium carbonate deposition |
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Application publication date: 20210709 |
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