CN113088444A - 使用电容耦合的电脉冲生成系统 - Google Patents

使用电容耦合的电脉冲生成系统 Download PDF

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Publication number
CN113088444A
CN113088444A CN202110196663.5A CN202110196663A CN113088444A CN 113088444 A CN113088444 A CN 113088444A CN 202110196663 A CN202110196663 A CN 202110196663A CN 113088444 A CN113088444 A CN 113088444A
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Prior art keywords
pulse generation
electrodes
generation system
electrical pulse
sample
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CN202110196663.5A
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English (en)
Chinese (zh)
Inventor
A.凯亚法
V.B.内库莱斯
A.L.加纳
A.S.托雷斯
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General Electric Co
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General Electric Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
    • A61B18/04—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating
    • A61B18/12—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating by passing a current through the tissue to be heated, e.g. high-frequency current
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/15—Devices for taking samples of blood
    • A61B5/150007—Details
    • A61B5/150755—Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • H03K3/02—Generators characterised by the type of circuit or by the means used for producing pulses
    • H03K3/53—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Immunology (AREA)
  • Ecology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Surgery (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medical Informatics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Plasma & Fusion (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Otolaryngology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202110196663.5A 2014-01-17 2014-11-20 使用电容耦合的电脉冲生成系统 Pending CN113088444A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/158106 2014-01-17
US14/158,106 US9708597B2 (en) 2014-01-17 2014-01-17 Electric pulse generation systems using capacitive coupling
CN201480073306.8A CN105899654A (zh) 2014-01-17 2014-11-20 使用电容耦合的电脉冲生成系统

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201480073306.8A Division CN105899654A (zh) 2014-01-17 2014-11-20 使用电容耦合的电脉冲生成系统

Publications (1)

Publication Number Publication Date
CN113088444A true CN113088444A (zh) 2021-07-09

Family

ID=52134369

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202110196663.5A Pending CN113088444A (zh) 2014-01-17 2014-11-20 使用电容耦合的电脉冲生成系统
CN201480073306.8A Pending CN105899654A (zh) 2014-01-17 2014-11-20 使用电容耦合的电脉冲生成系统

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Country Status (12)

Country Link
US (2) US9708597B2 (https=)
EP (1) EP3094969B1 (https=)
JP (1) JP6573617B2 (https=)
KR (2) KR102255951B1 (https=)
CN (2) CN113088444A (https=)
AU (1) AU2014377586B2 (https=)
BR (1) BR112016015394B1 (https=)
CA (1) CA2936197C (https=)
MX (1) MX377168B (https=)
RU (1) RU2709822C2 (https=)
TW (1) TWI673033B (https=)
WO (1) WO2015108619A1 (https=)

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Publication number Priority date Publication date Assignee Title
US9752120B2 (en) 2015-03-31 2017-09-05 General Electric Company Activated platelet composition with tunable growth factor level
US10633645B2 (en) 2015-12-30 2020-04-28 General Electric Company Calcium controlled activation of platelets via electrical stimulation
WO2017176253A1 (en) * 2016-04-05 2017-10-12 General Electiric Comapny Activated platelet composition with tunable growth factor level
US10233419B2 (en) 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
US10968423B2 (en) * 2018-02-26 2021-04-06 General Electric Company System and method for electric pulse based activation of biological samples
EP3908362A2 (en) * 2019-01-10 2021-11-17 Nanovis, LLC Methods for stimulating the proliferation and differentiation of eukaryotic cells
JP7248721B2 (ja) * 2021-02-17 2023-03-29 ゼネラル・エレクトリック・カンパニイ 成長因子レベルを調節可能な活性化血小板組成物
CN114062143B (zh) * 2021-11-30 2022-07-29 重庆大学 高压电脉冲原位增透含气储层两相渗流试验装置

Citations (6)

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US6010613A (en) * 1995-12-08 2000-01-04 Cyto Pulse Sciences, Inc. Method of treating materials with pulsed electrical fields
US6035237A (en) * 1995-05-23 2000-03-07 Alfred E. Mann Foundation Implantable stimulator that prevents DC current flow without the use of discrete output coupling capacitors
US20070231873A1 (en) * 2006-02-10 2007-10-04 Bio-Rad Laboratories, Inc. Multi-channel electroporation system
CN103052424A (zh) * 2010-06-04 2013-04-17 大学健康网络 功能性电刺激装置和系统及其用途
CN103502424A (zh) * 2011-03-03 2014-01-08 加利福尼亚大学董事会 用于操作细胞的纳米吸管装置
CN105899949A (zh) * 2014-01-17 2016-08-24 通用电气公司 使用电脉冲的血小板活化和生长因子释放

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US5186800A (en) * 1988-04-18 1993-02-16 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells
US5869326A (en) * 1996-09-09 1999-02-09 Genetronics, Inc. Electroporation employing user-configured pulsing scheme
EP0866123B1 (de) * 1997-03-21 2005-03-16 Eppendorf Ag Verfahren und Schaltungsanordnung zur Elektropermeation oder Elektroporation von lebenden Zellen
US6258592B1 (en) * 1999-06-14 2001-07-10 Bio-Rad Laboratories, Inc. Electroporation cell with arc prevention/reduction
US7053063B2 (en) * 1999-07-21 2006-05-30 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes in tissue
US6326177B1 (en) 1999-08-04 2001-12-04 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Method and apparatus for intracellular electro-manipulation
DE10119901A1 (de) * 2001-04-23 2002-10-24 Amaxa Gmbh Schaltungsanordnung zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms
JP4810425B2 (ja) * 2003-07-18 2011-11-09 イースタン バージニア メディカル スクール 電気パルスを発生するための装置および該装置を使用する方法
DE502004009820D1 (de) * 2004-03-15 2009-09-10 Lonza Cologne Ag Behältnis und Vorrichtung zur Erzeugung von elektrischen Feldern in einzelnen Reaktionsräumen
US6897069B1 (en) 2004-06-08 2005-05-24 Ambion, Inc. System and method for electroporating a sample
US7565201B2 (en) 2004-12-17 2009-07-21 Eastern Virginia Medical School Activation of calcium-mediated cell functions in cells and tissues, including aggregation of human platelets. by nanosecond pulsed electric fields
AU2006283551B2 (en) 2005-08-19 2011-07-21 Old Dominion Research Foundation Ultrawideband antenna for operation in tissue
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CN102209589A (zh) * 2008-11-06 2011-10-05 皇家飞利浦电子股份有限公司 试管和用于鉴定试管的方法
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KR101114712B1 (ko) * 2009-10-23 2012-02-29 세원셀론텍(주) 염화칼슘용액과 제1형 콜라겐으로 혈소판풍부혈장(ｐｒｐ)을 활성화하여 조직재생을 유도하는 조성물의 제조방법
US9078862B2 (en) 2013-06-06 2015-07-14 General Electric Company Platelet activation using long electric field pulses
US9238808B2 (en) 2013-06-06 2016-01-19 General Electric Company Modular adjustable pulse generator

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6035237A (en) * 1995-05-23 2000-03-07 Alfred E. Mann Foundation Implantable stimulator that prevents DC current flow without the use of discrete output coupling capacitors
US6010613A (en) * 1995-12-08 2000-01-04 Cyto Pulse Sciences, Inc. Method of treating materials with pulsed electrical fields
US20070231873A1 (en) * 2006-02-10 2007-10-04 Bio-Rad Laboratories, Inc. Multi-channel electroporation system
CN103052424A (zh) * 2010-06-04 2013-04-17 大学健康网络 功能性电刺激装置和系统及其用途
CN103502424A (zh) * 2011-03-03 2014-01-08 加利福尼亚大学董事会 用于操作细胞的纳米吸管装置
CN105899949A (zh) * 2014-01-17 2016-08-24 通用电气公司 使用电脉冲的血小板活化和生长因子释放

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肖华娟等: "纳秒脉冲细胞内电处理机理及其研究进展", 高电压技术, vol. 30, no. 04, pages 39 - 41 *

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Publication number Publication date
US10329551B2 (en) 2019-06-25
US20170314009A1 (en) 2017-11-02
RU2016127559A (ru) 2018-02-20
AU2014377586A1 (en) 2016-07-14
KR102255951B1 (ko) 2021-05-25
CN105899654A (zh) 2016-08-24
WO2015108619A1 (en) 2015-07-23
TW201545707A (zh) 2015-12-16
AU2014377586B2 (en) 2020-05-28
MX377168B (es) 2025-03-07
BR112016015394A8 (pt) 2020-06-02
KR20210014218A (ko) 2021-02-08
US9708597B2 (en) 2017-07-18
TWI673033B (zh) 2019-10-01
EP3094969B1 (en) 2019-09-11
KR20160108416A (ko) 2016-09-19
BR112016015394B1 (pt) 2021-01-12
JP2017505656A (ja) 2017-02-23
CA2936197A1 (en) 2015-07-23
JP6573617B2 (ja) 2019-09-11
EP3094969A1 (en) 2016-11-23
MX2016009314A (es) 2016-10-13
BR112016015394A2 (https=) 2017-08-08
RU2016127559A3 (https=) 2018-07-12
CA2936197C (en) 2023-04-25
US20150203836A1 (en) 2015-07-23
KR102213731B1 (ko) 2021-02-08
RU2709822C2 (ru) 2019-12-23

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