CN113082261A - Method for disinfecting livestock and poultry farm by utilizing strongly alkaline electrolyzed water - Google Patents
Method for disinfecting livestock and poultry farm by utilizing strongly alkaline electrolyzed water Download PDFInfo
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- CN113082261A CN113082261A CN202011131212.5A CN202011131212A CN113082261A CN 113082261 A CN113082261 A CN 113082261A CN 202011131212 A CN202011131212 A CN 202011131212A CN 113082261 A CN113082261 A CN 113082261A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/22—Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
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- Animal Behavior & Ethology (AREA)
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The invention relates to a method for disinfecting livestock and poultry farms by using strongly alkaline electrolyzed water, which comprises the following steps: step 1, obtaining alkaline stock solution by taking potassium carbonate as electrolyte through electrolysis, wherein the oxidation-reduction potential (ORP) value of the alkaline stock solution is as low as-1000 mV, and the pH value is 13.20-13.60; the electrolyzed water with strong reducibility has quick and broad-spectrum sterilization effect and is not easy to cause bacterial drug resistance; no chemical substance is added in the preparation process, so that skin and mucous membrane can not be burnt when the skin and mucous membrane are contacted, and the metal object is not corroded; oxidized into water in natural environment and sterilization process, and no chemical residue and environmental pollution exist; the strong alkaline electrolyzed water can be used as a safe, green and environment-friendly disinfectant for environment disinfection, air disinfection, drinking water disinfection and belt (livestock and poultry) disinfection of livestock and poultry farms.
Description
Technical Field
The invention relates to the technical field of livestock and poultry breeding, in particular to a method for disinfecting a livestock and poultry farm by utilizing strong alkaline electrolyzed water.
Background
In the production process of livestock and poultry farms, the important factor of economic loss of the farms caused by diseases is disinfection, which is an important means for eliminating pathogenic microorganisms in the external environment, cutting off the transmission path and controlling the occurrence of infectious diseases. The alkaline disinfectant is also one of the common disinfectants for livestock and poultry farms, and the alkaline disinfectant damages a bacterial enzyme system by dissolving protein. The higher the dissociation degree of hydroxide ions in the alkali liquor is, the stronger the antibacterial activity is; the alkali disinfectant has strong effect of killing virus, and the high-concentration alkali liquor can kill spores, so that the alkali disinfectant is mainly used for disinfecting viral infectious diseases. The alkali disinfectant is commonly used for disinfecting livestock and poultry farms, the ground of livestock houses, instruments and equipment, articles in houses, and excrement and waste containing pathogens generated in the livestock and poultry raising process, and mainly comprises sodium hydroxide (caustic soda ), calcium oxide (quicklime) and the like. The sodium hydroxide has strong killing power on bacteria and viruses, but has strong irritation on skins and mucous membranes of livestock and poultry or human bodies, and can cause skin and mucous membranes to burn and corrode and even damage tissues and organs due to excessive or long-term contact; if the fertilizer is used for a long time in a large dosage and high concentration, the waste liquid is not treated and is directly discharged into the environment, so that the pollution of water and soil is caused, the quality of peripheral crops and agricultural products is influenced, and even the health of human bodies is damaged; generally used for disinfection of barns, floors, utensils and the like contaminated by bacteria and viruses; is not suitable for sterilizing body surfaces of livestock and poultry, vehicles and metal articles.
Disclosure of Invention
Aiming at the defects of the prior art and the requirements of actual production, the invention provides a method for disinfecting livestock and poultry farms by using strongly alkaline electrolyzed water, wherein the alkaline electrolyzed water is electrolyzed water with pH value over 13 by electrolyzing electrolyte, the oxidation-reduction potential (ORP) value is as low as-1000 mV, and the electrolyzed water has strong reducibility, has quick and broad-spectrum sterilization effect and is not easy to cause bacterial drug resistance; the main component is water, and no chemical substance is added in the preparation process, so the alkaline electrolyzed water is harmless to the environment and human body, has small corrosion to equipment and strong decontamination capability; therefore, the strong alkaline electrolyzed water can be used as a safe, green and environment-friendly disinfectant for the field disinfection, the air disinfection and the belt (livestock) disinfection of livestock and poultry farms.
In order to achieve the above objects, there is provided a method for sterilizing a livestock and poultry farm using strongly alkaline electrolyzed water, the method comprising the steps of:
step 1, obtaining alkaline stock solution by taking potassium carbonate as electrolyte through electrolysis, wherein the oxidation-reduction potential (ORP) value of the alkaline stock solution is as low as-1000 mV, and the pH value is 13.20-13.60;
step 2, diluting the alkaline stock solution by 100-250 times to obtain electrolyzed water with the pH value of 10.50-11.20, and using the electrolyzed water for sterilizing environments such as walkways, floors, facilities, roofs and walls in the livestock and poultry breeding house;
step 3, diluting the alkaline stock solution by 500 times to obtain electrolytic water with the pH value larger than 10, then spraying the diluted electrolytic water to the whole pigsty space by using atomization spraying equipment, wherein the spraying time is 0.5-3min and 2-5 times a day, and the conventional bacteria are killed, and the stink is removed;
step 4, diluting the alkaline stock solution by 5000 times to obtain electrolyzed water with the pH value of 7.80-9.80 for livestock and poultry drinking water in livestock and poultry farms, and blocking the propagation of pathogenic microorganisms through the drinking water;
and 5, diluting the alkaline stock solution by 30-50 times to obtain electrolyzed water with the pH value of more than 11, and using the electrolyzed water as a waterline of the livestock and poultry farm for cleaning, removing dirt inside the water pipe and killing germs inside the water pipe.
Further, according to the step 2, the alkaline stock solution is diluted to 130-200 times to obtain electrolyzed water with the pH value of 10.8-11.
Further, the piggery is sprayed for 3-4 times per day according to the step 3, and the spraying time is 1-2min each time.
Further, the alkaline stock solution in the step 4 is diluted by 3000-4000 times to obtain electrolyzed water with the pH value of 8.20-9.40.
Further, the in-vitro antibacterial effect of the alkaline electrolyzed water on the haemophilus parasuis is measured by a 96-well plate method.
Further, the alkaline stock solution is diluted by the culture solution in a multiple ratio and added into a 96-well plate, and then diluted bacterium solution is added to ensure that the concentration of the bacterium solution in each hole is 1 multiplied by 106And (3) CFU/mL, putting the 96-well plate into a constant temperature box, incubating for 24h at 37 ℃, taking out, recording the absorbance (OD) value of the solution at 600nm through an enzyme-labeling instrument, and calculating the inhibition rate of alkaline electrolyzed water on the streptococcus suis and the haemophilus parasuis.
The invention has the following beneficial effects:
1. the alkaline electrolyzed water is electrolyzed water with pH value over 13 strong basicity obtained by electrolyzing electrolyte, and oxidation-reduction potential (ORP) value is as low as-1000 mV, so that the electrolyzed water has strong reducibility, has quick and broad-spectrum sterilization effect and is not easy to cause bacterial drug resistance;
2. the main component of the alkaline electrolyzed water is water, no chemical substance is added in the preparation process, skin and mucous membrane can not be burnt when the alkaline electrolyzed water is contacted, and the alkaline electrolyzed water has no corrosion to metal articles; oxidized into water in natural environment and sterilization process, and no chemical residue and environmental pollution exist;
3. the strong alkaline electrolyzed water can be used as a safe, green and environment-friendly disinfectant to be applied to the environmental disinfection, air disinfection, drinking water disinfection and belt (livestock and poultry) disinfection of livestock and poultry farms;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for disinfecting livestock and poultry farms by using strongly alkaline electrolyzed water comprises the following steps:
step 1, obtaining an alkaline stock solution by taking potassium carbonate as electrolyte through electrolysis, wherein the oxidation-reduction potential (ORP) value of the alkaline stock solution is as low as-1000 mV, and the pH value is 13.20;
step 2, diluting the alkaline stock solution by 100 times to obtain electrolyzed water with the pH value of 10.50, and using the electrolyzed water for sterilizing the environments such as walkways, floors, facilities, roofs and walls in the livestock and poultry breeding house;
step 3, diluting the alkaline stock solution by 500 times to obtain electrolytic water with the pH value larger than 10, and then spraying the diluted electrolytic water to the whole pigsty space by using atomization spraying equipment, wherein the time of spraying is 2 times a day, and each time is 3min, so that conventional bacteria are killed, and offensive odor is removed;
step 4, diluting the alkaline stock solution by 2000 times to obtain electrolyzed water with the pH value of 9.80, wherein the electrolyzed water is used as drinking water for livestock and poultry in livestock and poultry farms, and pathogenic microorganisms are blocked from spreading through the drinking water;
and 5, diluting the alkaline stock solution by 30-50 times to obtain electrolyzed water with the pH value of more than 11, and using the electrolyzed water as a waterline of the livestock and poultry farm for cleaning, removing dirt inside the water pipe and killing germs inside the water pipe.
Example 2
A method for disinfecting livestock and poultry farms by using strongly alkaline electrolyzed water comprises the following steps:
step 1, obtaining an alkaline stock solution by taking potassium carbonate as electrolyte through electrolysis, wherein the oxidation-reduction potential (ORP) value of the alkaline stock solution is as low as-1000 mV, and the pH value is 13.4;
step 2, diluting the alkaline stock solution by 200 times to obtain electrolyzed water with the pH value of 11.0 for sterilizing the environments such as walkways, floors, facilities, roofs and walls in the livestock and poultry breeding house;
step 3, diluting the alkaline stock solution by 500 times to obtain electrolytic water with the pH value larger than 10, and then spraying the diluted electrolytic water to the whole pigsty space by using atomization spraying equipment, wherein the time of spraying is 3 times a day, and each time is 1min, so that conventional bacteria are killed, and offensive odor is removed;
step 4, diluting the alkaline stock solution by 3000 times to obtain electrolyzed water with the pH value of 8.60, wherein the electrolyzed water is used as drinking water for livestock and poultry in livestock and poultry farms, and pathogenic microorganisms are blocked from spreading through the drinking water;
step 5, diluting the alkaline stock solution by 30-50 times to obtain electrolyzed water with the pH value larger than 11, and using the electrolyzed water as a waterline of the livestock and poultry farm for cleaning, removing dirt inside the water pipe, and killing germs inside the water pipe
Example 3
A method for disinfecting livestock and poultry farms by using strongly alkaline electrolyzed water comprises the following steps:
step 1, obtaining an alkaline stock solution by taking potassium carbonate as electrolyte through electrolysis, wherein the oxidation-reduction potential (ORP) value of the alkaline stock solution is as low as-1000 mV, and the pH value is 13.60;
step 2, diluting the alkaline stock solution by 250 times to obtain electrolyzed water with the pH value of 11.20, and using the electrolyzed water for sterilizing the environments such as walkways, floors, facilities, roofs and walls in the livestock and poultry breeding house;
step 3, diluting the alkaline stock solution by 500 times to obtain electrolytic water with the pH value larger than 10, and then spraying the diluted electrolytic water to the whole pigsty space by using atomization spraying equipment, wherein the time of spraying is 5 times a day, and each time of spraying is 0.5min, so that conventional bacteria are killed, and offensive odor is removed;
step 4, diluting the alkaline stock solution by 5000 times to obtain electrolyzed water with the pH value of 7.80, wherein the electrolyzed water is used as drinking water for livestock and poultry in livestock and poultry farms, and pathogenic microorganisms are blocked from spreading through the drinking water;
and 5, diluting the alkaline stock solution by 30-50 times to obtain electrolyzed water with the pH value of more than 11, and using the electrolyzed water as a waterline of the livestock and poultry farm for cleaning, removing dirt inside the water pipe and killing germs inside the water pipe.
PH values of different dilution concentrations of alkaline electrolyzed water
| Dilution factor | PH value (repeat one) | PH value (repeat two) |
| Stock solution | 13.60 | 13.57 |
| 2 times of | 13.06 | 13.03 |
| 4 times of | 12.67 | 12.76 |
| 8 times of | 12.26 | 12.43 |
| 16 times of | 11.99 | 12.05 |
| 32 times of | 11.64 | 11.60 |
| 64 times of | 11.36 | 11.16 |
| 128 times of | 11.23 | 10.97 |
| 256 times of | 10.71 | 10.51 |
| 512 times of | 10.46 | 9.97 |
| 1024 times | 10.15 | 9.45 |
| 2048 times of | 9.85 | 8.98 |
| 4096 times that of | 7.92 | 7.82 |
Measuring the in vitro antibacterial effect of alkaline electrolyzed water on Haemophilus parasuis by using a 96-well plate method, diluting an alkaline stock solution by using a culture solution in a multiple ratio, sequentially adding the diluted stock solution into a 96-well plate, and adding the diluted bacterial solution to ensure that the concentration of each pore of the bacterial solution is 1 multiplied by 106And CFU/m, putting the 96-well plate into a constant temperature box, incubating for 24h at 37 ℃, taking out, recording the absorbance (OD) value of the solution at 600nm through an enzyme-labeling instrument, and calculating the inhibition rate of alkaline electrolyzed water on haemophilus parasuis.
Bacteriostatic effect of alkaline electrolyzed water on haemophilus parasuis under different dilution concentrations
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A method for disinfecting livestock and poultry farms by using strongly alkaline electrolyzed water is characterized by comprising the following steps:
step 1, obtaining alkaline stock solution by taking potassium carbonate as electrolyte through electrolysis, wherein the oxidation-reduction potential (ORP) value of the alkaline stock solution is as low as-1000 mV, and the pH value is 13.20-13.60;
step 2, diluting the alkaline stock solution by 100-250 times to obtain electrolyzed water with the pH value of 10.50-11.20, and using the electrolyzed water for sterilizing environments such as walkways, floors, facilities, roofs and walls in the livestock and poultry breeding house;
step 3, diluting the alkaline stock solution by 500 times to obtain electrolytic water with the pH value of more than 10, and then spraying the diluted electrolytic water to the whole pigsty space by using atomization spraying equipment, wherein the time of spraying is 2-5 times per day, and the time of spraying is 0.5-3min per time, so that conventional bacteria are killed, and offensive odor is removed;
step 4, diluting the alkaline stock solution by 5000 times to obtain electrolyzed water with the pH value of 7.80-9.80 for livestock and poultry drinking water in livestock and poultry farms, and blocking the propagation of pathogenic microorganisms through the drinking water;
and 5, diluting the alkaline stock solution by 30-50 times to obtain electrolyzed water with the pH value of more than 11, and using the electrolyzed water as a waterline of the livestock and poultry farm for cleaning, removing dirt inside the water pipe and killing germs inside the water pipe.
2. The method for disinfecting a livestock and poultry farm by using strongly alkaline electrolyzed water according to claim 1, wherein the electrolyzed water having a pH value of 10.8-11 is obtained by diluting the alkaline stock solution to 130-200 times according to step 2.
3. The method for disinfecting a livestock and poultry farm by using strongly alkaline electrolyzed water according to claim 1, wherein the piggery is sprayed 3 to 4 times per day for 1 to 2min according to step 3.
4. The method for disinfecting a livestock and poultry farm by using strongly alkaline electrolyzed water according to claim 1, wherein the alkaline stock solution is diluted 3000-4000 times in the step 4 to obtain electrolyzed water with a pH value of 8.20-9.40.
5. The method according to claim 1, wherein the in vitro antibacterial effect of the alkaline electrolyzed water on haemophilus parasuis is measured by a 96-well plate method.
6. The method according to claim 5, wherein the alkaline stock solution is diluted by a culture solution and added to a 96-well plate, and then diluted bacteria solution is added so that the concentration of bacteria solution per well is 1 x 106And CFU/mL, putting the 96-well plate into a constant temperature box, incubating for 24h at 37 ℃, taking out, recording the absorbance (OD) value of the solution at 600nm through an enzyme-labeling instrument, and calculating the inhibition rate of alkaline electrolyzed water on haemophilus parasuis.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113729039A (en) * | 2021-08-27 | 2021-12-03 | 上海罗克环控节能科技股份有限公司 | Green environment-friendly disinfectant and preparation method thereof |
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