CN113074860A - Miniature pressure sensing device for blood vessel chip - Google Patents
Miniature pressure sensing device for blood vessel chip Download PDFInfo
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- CN113074860A CN113074860A CN202110304902.4A CN202110304902A CN113074860A CN 113074860 A CN113074860 A CN 113074860A CN 202110304902 A CN202110304902 A CN 202110304902A CN 113074860 A CN113074860 A CN 113074860A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
- G01L9/00—Measuring steady of quasi-steady pressure of fluid or fluent solid material by electric or magnetic pressure-sensitive elements; Transmitting or indicating the displacement of mechanical pressure-sensitive elements, used to measure the steady or quasi-steady pressure of a fluid or fluent solid material, by electric or magnetic means
- G01L9/0041—Transmitting or indicating the displacement of flexible diaphragms
- G01L9/0051—Transmitting or indicating the displacement of flexible diaphragms using variations in ohmic resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The invention relates to a micro pressure sensing device for measuring the local pressure of a vascular chip, which is characterized in that the upper layer is a cell culture layer and comprises a vascular chip cell culture channel, and the lower layer of the channel is used for fixedly culturing sample cells to be detected. Under the action of a peripheral pressure pump, a complex and changeable mechanical microenvironment experienced by vascular cells can be simulated; the middle layer is a thin film sensing layer, the thin film sensing layer is elastically deformed under pressure so as to change a local composite material area in the thin film sensing layer, namely a local silver nanowire/polydimethylsiloxane composite elastic thin film resistor, and the connecting wire and a universal meter can directly measure the resistance value by utilizing the characteristics of high precision and excellent conductivity of a PDMS thin film mixed with AgNWs under low pressure; the lower layer is an air layer and comprises a sensor lower layer air channel, and the channel can provide a space for the deformation of the thin film sensing layer. The device provided by the invention is simple to fuse with the vascular chip, has low cost and can realize large-scale integrated application.
Description
Technical Field
The invention relates to a micro pressure sensing device for measuring local pressure in a micro channel by an organ chip under a micro scale in medical biomechanics, in particular to a micro pressure sensing device for measuring the local pressure of a vascular chip.
Background
In the field of biomedical research, the microfluidic technology provides a new technical platform for realizing a complex spatial structure of in-vitro living tissue by virtue of the characteristics of high sensitivity, low consumption, integration, controllability and the like, and pressure measurement in a microfluidic system plays an important role in biology and biomedicine. For example, in the heart cell culture, the pressure around the heart cells is measured by using an external pressure sensor on the basis of cell morphology, and then the gene condition of a human body and the contraction function of the cells are analyzed; the lung chip micro-device can simulate the key structure and function of a human lung unit, endothelial cells and epithelial cells are induced and cultured in the lung chip micro-device, and the mechanical characteristics of a human alveolar-capillary interface can be reproduced.
Silver nanowires (AgNWs) are a novel nanomaterial, combines the excellent conductivity of silver and the size effect of the nanowires, has excellent light transmittance, and is widely applied to flexible transparent electrodes; meanwhile, the percolation network formed of AgNWs possesses excellent flexibility, can accommodate large deformation, exhibits superior electrical conductivity and deformability, and is thus also used to prepare stretchable electronic devices.
Mechanics and biology research shows that in-vivo mechanics microenvironment of vascular cells participates in regulation and control of wall reconstruction, and local abnormal mechanical stimulation can cause abnormal cell function and induce cardiovascular diseases such as aneurysm, atherosclerosis and the like. The stress-growth relationship curve proposed by Mr. von Neuman Frames shows that cell growth is not linearly related to the magnitude of stress it is subjected to, and that mechanical stimuli that are too high or too low may cause completely different cellular responses. Therefore, to study a specific pathological mechanism, a corresponding device is required to test the effect of the pressure effect of the fluid on cell growth. In the literature (research progress of a pressure sensing technology in a microfluidic system, 2019, volume 38, ninth phase), Habibi M and the like use a surface micromachining method to manufacture a capacitive pressure sensor array on a glass substrate, the electrical pressure sensor has a complex process and high cost, requires metal deposition, etching and other steps, and generally uses materials with poor mechanical properties such as silicon, carbon fiber and the like, so that the sensor is damaged when being bent or impacted. Song W Z et al propose a chip-scale optical fluidic interferometric sensor for measuring fluid pressure based on an imaging method, by imaging and analyzing the interference pattern, which requires large-scale instrumentation to acquire images and perform complex analysis to estimate pressure. Jung T uses gallium indium tin liquid metal and a resistance pressure sensing technology to integrate pressure sensing in a micro-fluidic system by using a soft lithography technology, and prepares a micro-fluidic pressure sensor based on a thin film, and the device has strict requirements on sealing property, and is easy to cause pollution problems if metal liquid leaks.
Therefore, it is necessary to develop a micro pressure sensing device for a vascular chip, which can be easily integrated with a microfluidic chip, has low cost, can be applied on a large scale, is integrated with an AgNWs-based stretchable conductive film, has high precision and excellent conductivity under low pressure, can be expanded into a multifunctional sensor, and is a good choice for measuring the pressure in a microchannel.
Disclosure of Invention
The present invention has been made to solve the above-mentioned problems occurring in the prior art. In particular, the invention aims to solve the problems of low practicability, high cost, complex operation and the like of the pressure sensor of the existing microfluidic system.
The invention provides a micro pressure sensing device for a vascular chip, which comprises: the device comprises a cell culture layer 1, a vascular chip cell culture channel 2, a film sensing layer 3, a local AgNWs/PDMS composite elastic film 4, an external electric wire 5, an air layer 6 and a sensor lower-layer air channel 7.
The invention provides a micro pressure sensing device for a vascular chip, which is characterized by comprising three parts: the first part is a cell culture layer 1 which comprises a blood vessel chip cell culture channel 2, a liquid inlet pump is introduced into culture solution, blood vessel cells are fixedly cultured on the lower surface layer of the blood vessel chip cell culture channel 2, and different mechanical microenvironments are provided by taking a pressure pump as a power source, so that inoculation, long-term culture and cytological stimulation of the blood vessel cells are integrated in the blood vessel chip cell culture channel 2; the second part is a film sensing layer 3 which comprises a local AgNWs/PDMS composite elastic film 4 and an external electric wire 5, the film sensing layer 3 is elastically deformed under the action of pressure, meanwhile, the growth condition of vascular cells is influenced by different pressure values, the electric conduction measurement can be realized by utilizing the excellent electric conductivity of the local composite elastic film, the external electric wires 5 on the two sides of the local AgNWs/PDMS composite elastic film 4 are connected with a universal meter to convert the deformation quantity of the local AgNWs/PDMS composite elastic film 4 into a corresponding resistance value, and therefore, the change of a local mechanical microenvironment of sample cells in an upper vascular chip cell culture channel can be monitored by monitoring the resistance change of the local AgNWs/PDMS composite elastic film; the third part is an air layer 6 comprising sensor sub-layer air channels 7 that provide space for the deformation of the thin film sensing layer 3.
The invention specifically provides a miniature pressure sensing device for a vascular chip, which is characterized in that: the micro-fluidic chip system integrates the inoculation of cells and the mechanical stimulation of the cells on a functional chip, so that the consumption of the cells and reagents is low, and the operation is simple and convenient; the system consists of two basic units: the first basic unit is a blood vessel chip for researching cell stress behavior, which comprises a cell culture layer and a blood vessel chip cell culture channel; the second basic unit is a vascular chip pressure sensing research device which comprises a film sensing layer, a local silver nanowire/polydimethylsiloxane composite elastic film, an external wire, an air layer, a sensor lower-layer air channel and observation peripheral equipment.
The invention provides a miniature pressure sensing device for a vascular chip, which is used for observing peripheral equipment and mainly comprises a culture solution inlet pump, a pressure pump, a microscope and a universal meter. The method is characterized in that different local mechanical microenvironments in the blood vessel are simulated by adjusting the power provided by the pressure pump; the microscope can be used for observing and recording the growth characteristics of the vascular cells under different mechanical stimuli in the vascular chip; the multimeter is connected with an external wire to realize the measurement of the transformation of the deformation quantity of the thin film sensing layer into the resistance quantity.
In a preferred embodiment of the above micro pressure sensing device, the cell culture layer and the vascular chip cell culture channel are disposed on the upper layer of the thin film sensing layer and the local AgNWs/PDMS composite elastic film, and the air layer and the sensor air layer channel are disposed on the lower layer of the thin film sensing layer and the local AgNWs/PDMS composite elastic film.
In the preferred embodiment of the above micro pressure sensing device, the thickness dimension of the local AgNWs/PDMS composite elastic film is allowed to be in the range of 10 micrometers to 200 micrometers.
The invention provides a micro pressure sensing device for a blood vessel chip, which is characterized in that the width and height of a blood vessel chip cell culture channel and a sensor air layer channel are 10 micrometers to 1 millimeter, and the cross part of a film and an upper channel and a lower channel is rectangular or circular.
The invention provides a micro pressure sensing device for a vascular chip, which is characterized in that the material parts of a channel, a cell culture layer, an air layer and a film sensing layer are Polydimethylsiloxane (PDMS) polymer, and the pressure sensitive element part is AgNWs/PDMS composite polymer.
From the above description, it can be seen that the invention makes full use of the advantages of good light transmittance, low thermal expansion coefficient, strong bond and performance, good thermal stability, good chemical inertness and high biocompatibility of the organic polymer material PDMS to make the channel wall, and the PDMS material has low cost and good adhesion with the silicon wafer, and can be suitable for online real-time observation and detection by utilizing the optically transparent performance thereof. In the research of cell behaviors, accurate pressure measurement is the key for applying mechanical force and monitoring the occurrence of accidents, silver nanowires (AgNWs) are combined with the excellent conductivity of silver and the size effect of the nanowires at the same time, and a local AgNWs/PDMS composite elastic film prepared by mixing the silver nanowires with PDMS can convert the deformation quantity of a film sensing layer into a resistance value to be output, so that accurate local pressure measurement of a vascular chip is realized.
Experimental measurements of a vascular system of interest have shown that there are differences in the body load experienced by blood vessels at different locations. The micro-fluidic chip system for researching cell stress behaviors provided by the invention can simply observe the growth conditions of cells in microenvironments with different stresses in real time, and realize the in vitro simulation of the mechanical property differences presented by different physiological parts of blood vessels and the complex and changeable mechanical microenvironments experienced by blood vessel cells. External wires on two sides of the local AgNWs/PDMS composite elastic film can be used for measuring a real-time resistance value by using a universal meter; due to the transparent characteristic of the PDMS material, the biological characteristics of the cells of the fixed culture sample in the blood vessel chip under the pressure value can be directly observed through a microscope, and the influence of local abnormal mechanical stimulation on the functions of the blood vessel cells can be directly, simply and conveniently researched in low cost by combining with software analysis and processing, so that the research on the reasons for inducing the cardiovascular diseases such as aneurysm, atherosclerosis and the like is facilitated.
It will be readily appreciated by those skilled in the art that the measurement device and method of the present invention have a number of advantages due to the full use of the organic polymer materials PDMS and AgNWs; 1) the local pressure on the cells fixedly cultured in the vascular chip can be monitored in real time, the micro pressure sensing device can identify the change of smaller pressure, the linearity is good, the response speed is high, and the reading is visual; 2) the signal conversion circuit has a signal conversion function and is convenient to integrate with a subsequent signal processing circuit; 3) the method is easy to miniaturize, and can be used for preparing chips with complex detection functions and high integration level; 4) the silver nanowires (AgNWs) combine the excellent conductivity of silver and the size effect of the nanowires at the same time, a seepage network formed by the AgNWs has excellent flexibility, and the AgNWs-based stretchable conductive film is blended into the AgNWs-based stretchable conductive film, so that the device is high in precision and excellent in conductivity under low pressure and can be expanded into a multifunctional sensor, and therefore the device can be applied to the field of preparing stretchable electronic equipment and the like.
Drawings
FIG. 1 shows a micro pressure sensing device for vascular chips according to the present invention, wherein 1 is a cell culture layer, 2 is a vascular chip cell culture channel, 3 is a thin film sensing layer, 4 is a local AgNWs/PDMS composite elastic thin film, 5 is an external wire, 6 is an air layer, and 7 is a sensor lower air channel;
fig. 2 shows a flow chart of a micro pressure sensing device for vascular chips according to the present invention.
Detailed Description
The technical solution of the present invention will be described below in connection with preferred embodiments, however, it is easily understood by those skilled in the art that the technical solution of the present invention is not limited to various details in the embodiments. Without deviating from the principle of the invention, namely a microchannel wall and a local AgNWs/PDMS composite elastic film made of PDMS and AgNWs materials, the local AgNWs/PDMS composite elastic film generates different deformations under different mechanical microenvironments provided by a pressure pump so as to cause different mechanical stimuli to blood vessel cells fixedly cultured on the lower layer of a blood vessel chip cell culture channel, and the magnitude of the measured real-time mechanical stimuli can be obtained by utilizing the conductivity of the composite film. Various changes may be made to the described embodiments without requiring any inventive effort in the context of monitoring the local pressure of the fluid and are therefore intended to fall within the scope of the present invention.
The invention designs a micro pressure sensor integrated in a multi-layer structure of a microfluidic chip for a vascular chip independently, which is characterized in that after a culture solution is introduced into a cell culture channel of an upper vascular chip, a pressure pump generates stable pressure to extrude a film sensing layer and a local AgNWs/PDMS composite elastic film, the composite elastic film is arranged in an area where the film sensing layer generates the most obvious elastic deformation, the strain of different degrees corresponds to the change of different resistance values, the composite elastic film can be used for measuring the resistance by externally connecting wires at two sides of the local AgNWs/PDMS composite elastic film in a conductive manner, the fitting relation between the resistance value and the pressure is obtained, the obtained relation can be used for calculating the size of mechanical stimulation on a fixedly cultured sample cell to be detected, and then the growth conditions of the cells at different mechanical stimulation positions are compared and observed.
Specifically, as shown in fig. 1, the micro pressure sensing device of the present invention includes: the vascular chip cell culture channel in the cell culture layer is used for introducing culture solution by liquid inlet pump, in the preferred embodiment the culture solution is made up by using complete DMEM culture medium, contains 10% fetal calf serum, 100U/ml penicillin and 100ug/ml streptomycin, and can be cultured at 37 deg.C and 5% carbon dioxide saturated humidity, and the culture medium can be changed every two to three days, and its inoculation density can be 1X 105One per ml. The prepared chip is sequentially rinsed by 75% ethanol, sterile secondary water, PBS and alpha-EME culture medium containing 15% fetal bovine serum, and the vascular cells are fixed on the lower surface layer of the cell culture channel of the vascular chip after ensuring that no bubble residue exists in the channel.
The method for manufacturing the structural layer of the micro pressure sensing device by using the PDMS material is given as follows: setting the cell culture layer with a smaller thickness and a thicker air layer, preparing a blood vessel chip cell culture channel of the cell culture layer and a sensor lower-layer air channel of the air layer on a template prepared by photoetching in a reverse mold mode, and bonding the cell culture layer, the thin film sensing layer and the air layer by using a surface plasma cleaning machine.
The preparation method of the thin film sensing layer and the local AgNWs/PDMS composite elastic thin film is given as follows: mixing the PDMS elastomer and the curing agent according to the mass ratio of 10:1, stirring for 30min, then evacuating for 15min to remove internal bubbles, and dispersing AgNWs prepared by a hydrothermal method in ethanol, wherein the concentration of the AgNWs is 0.3 mg/mL. Then 0.2 wt% of AgNWs solution was added to 1g of PDMS mixture, and the mixture was stirred well with a magnetic bar to obtain composite solutions with different doping ratios. Centrifuging the obtained solution at 8000rpm for 5min, removing supernatant, stirring the composite solution for 20min, evacuating at 50 ℃ for 15min, finally spin-coating the composite solution on a PDMS material with silanized surface at 1000rpm by a spin coater, curing at 80 ℃ for 2 hours to obtain a thin film with the thickness of about 104 microns, cutting out a rectangle with the length and width of about 2cm and 5mm by a knife, reserving the rectangular PDMS/AgNWs composite polymer, digging out the rest part for later use, pouring pure PDMS polymer into the filled dug-out part, and further obtaining the thin film sensing layer and the partial AgNWs/PDMS composite elastic thin film.
Referring now to FIG. 2, a flow chart of a method of a micro pressure sensing device for a vascular chip in accordance with the present invention is shown. Comprising steps 100-104. Specifically, the method comprises the following steps:
and step 100, providing the micro pressure sensing device for the blood vessel chip.
And 102, applying stable mechanical stimulation to the sample cells to be detected. Specifically, air is introduced into a cell culture channel of the vascular chip by using a peripheral equipment pressure pump, so that cells of a sample to be detected which is fixedly cultured on the lower surface layer can be in different mechanical microenvironments to influence cell growth and biological characteristics, and meanwhile, the film sensing layer and the local AgNWs/PDMS composite elastic film are elastically deformed under pressure.
And 103, measuring the resistance value of the local AgNWs/PDMS composite elastic film. Specifically, wires are attached to two sides of the local AgNWs/PDMS composite elastic film, the local AgNWs/PDMS composite elastic film is elastically deformed under pressure, the resistance value of the local AgNWs/PDMS composite elastic film is correspondingly changed, and the wires are connected with a universal meter to perform resistance measurement on the conductive local AgNWs/PDMS composite elastic film.
And 104, analyzing the local pressure value of the blood vessel chip and observing the cell morphology and the biological characteristics. Specifically, the mechanical environment pressure value of the microchannel cell is obtained through the deduced conversion relation between the deformation quantity and the resistance value of the film, and meanwhile, the real-time growth condition of the cell can be observed by using a microscope.
Therefore, the change of the local mechanical microenvironment of the sample cells in the upper vascular chip cell culture channel can be monitored by monitoring the resistance change of the local AgNWs/PDMS composite elastic film. And because of the transparent characteristic of the PDMS material, the biological characteristic of the fixed culture sample cells in the blood vessel chip under the pressure value can be directly observed, so that researchers can conveniently research the influence of the pressure action of the fluid on the growth of the blood vessel cells. The miniature pressure sensing device can identify the change of small pressure, and has the advantages of good linearity, excellent response speed and visual reading.
So far, the technical solution of the present invention has been described with reference to the preferred embodiments and the flow charts shown in the drawings. The protection scope of the present invention is not limited to the above-mentioned embodiments, and those skilled in the art can make equivalent changes to the related technical features and parameters without departing from the principle of the present invention, and the technical solutions after the changes will fall into the protection scope of the present invention.
Claims (6)
1. A miniature pressure sensing device for vascular chips, comprising three parts: the first part is a cell culture layer and comprises a vascular chip cell culture channel; the second part is a film sensing layer which comprises a local silver nanowire/polydimethylsiloxane (AgNWs/PDMS) composite elastic film and an external wire; the third part is an air layer and comprises a sensor lower-layer air channel, wherein the width of the blood vessel chip cell culture channel is equal to that of the sensor lower-layer air channel;
fixedly culturing sample cells to be detected on the lower layer of a blood vessel chip cell culture channel, applying pressure to the sample cells in the blood vessel chip cell culture channel by using a pressure pump, and simultaneously, elastically deforming a film sensing layer, wherein a local silver nanowire/PDMS composite elastic film in a composite material area in the film sensing layer has the conductive characteristic, the composite elastic film is arranged in the area where the film sensing layer is most obviously elastically deformed, the resistance value of the local composite elastic film is correspondingly changed along with the change of pressure, and an external electric wire is connected with a universal meter to measure the resistance value; the air layer is connected with the film sensing layer, and a space for the film to deform under pressure is provided in the air channel under the sensor.
2. The micro pressure sensing device for blood vessel chip as claimed in claim 1, wherein the peripheral pressure pump is controlled to make the blood vessel cells respectively in vivo growth environments with different mechanical stimuli, so as to simulate complex and changeable mechanical microenvironments experienced by the blood vessel cells, and facilitate the study of growth characteristic differences exhibited by the blood vessel cells at different physiological sites.
3. The thin film sensing layer of claim 1, which can realize micro pressure sensing, is characterized in that the sensing layer is provided with a conductive composite material region, i.e. a local silver nanowire/polydimethylsiloxane composite elastic thin film, which is a sensing element of sensing, and the resistance value can be measured by utilizing the characteristics of high precision and excellent conductivity of the PDMS thin film of the mixed AgNWs under low pressure, so as to obtain the pressure value in the cell culture channel of the vascular chip.
4. The miniature pressure sensing device for vascular chip of claim 1, wherein the material of the cell culture layer, the air layer and the thin film sensing layer is polydimethylsiloxane, wherein the PDMS elastomer and the curing agent can be mixed and prepared according to the mass ratio of 7:1 to 15: 1; in the thin film sensing layer, a conductive composite elastic thin film can be prepared by a composite solution prepared by mixing AgNWs solution with the concentration of 0.2-1.0 wt% and PDMS, and the thickness of the prepared local AgNWs/PDMS composite elastic thin film is in the range of 10-200 micrometers; the width and height of the blood vessel chip cell culture channel in the cell culture layer and the lower air channel of the sensor in the air layer are 10 micrometers to 1 millimeter, and the cross part of the thin film sensing layer and the upper and lower channels is rectangular or circular.
5. The miniature pressure sensing device for vascular chips of claim 1, wherein different mechanical microenvironments are provided by adjusting a pressure pump to generate different pressures on the thin film sensing layer, so as to adjust the deformation degree of the local AgNWs/PDMS composite elastic thin film, and the resistance value of the composite elastic thin film corresponding to strain can be obtained by connecting an external electric wire with a multimeter.
6. The miniature pressure sensing device for vascular chips of claim 1, wherein said sample to be detected is a vascular cell.
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| CN113865760A (en) * | 2021-09-24 | 2021-12-31 | 国科温州研究院(温州生物材料与工程研究所) | Preparation method of anisotropic structural color film for myocardial mechanics sensing |
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