CN113072849A

CN113072849A - Functional monoatomic coating for preventing and controlling African swine fever virus and preparation method thereof

Info

Publication number: CN113072849A
Application number: CN202110353117.8A
Authority: CN
Inventors: 赵超; 王晶; 黄红锋; 吴宇波
Original assignee: Liankehua Technology Co Ltd
Current assignee: Liankehua Technology Co Ltd
Priority date: 2021-04-01
Filing date: 2021-04-01
Publication date: 2021-07-06

Abstract

The application relates to the field of special functional coatings, and particularly discloses a functional monoatomic coating for preventing and controlling African swine fever viruses and a preparation method thereof. A functional monoatomic coating for preventing and controlling African swine fever virus comprises the following raw materials: acrylate emulsion, a defoaming agent, a dispersing agent, propylene glycol, a film forming aid, a thickening agent, a pH regulator, a monoatomic antibacterial disinfectant, filler powder and deionized water. The single-atom catalyst capable of resisting the African swine fever virus is independently researched and developed, the African swine fever virus can be effectively killed, the African swine fever virus resisting rate is more than 99.9%, the lasting action time is long, and common pathogenic microorganisms in pig farms such as haemophilus parasuis, African swine fever, circovirus and porcine reproductive and respiratory syndrome virus can be effectively killed. The functional monoatomic coating for preventing and controlling the African swine fever virus, which is prepared by the application, can be specially used in animal farms such as pig raising and the like, and can be portably coated on the surfaces of buildings such as a hog house, a delivery room and the like, so that the African swine fever virus can be effectively subjected to biological safety protection.

Description

Functional monoatomic coating for preventing and controlling African swine fever virus and preparation method thereof

Technical Field

The invention relates to the field of special functional coatings, in particular to a functional monoatomic coating for preventing and controlling African swine fever viruses and a preparation method thereof.

Background

The coating is a continuous film which is coated on the surface of a protected or decorated object and can be firmly attached to the object to be coated, is closely related to the healthy life of people, and the functional coating increasingly enters the life of people along with the development of economy and the change of the consumption concept of people.

Therefore, the development of the coating product with the anti-African swine fever virus effect has great significance, and especially aiming at special functional products of livestock farms, the biological safety protection can be effectively improved, and the influence of pathogenic microorganisms can be reduced.

Disclosure of Invention

Aiming at the problem of serious harm to human society and life health caused by the epidemic situation of the African swine fever, the application aims to provide the functional monoatomic coating for preventing and controlling the African swine fever virus and the preparation method thereof.

In a first aspect, the application provides a functional monoatomic coating for preventing and controlling african swine fever virus, which adopts the following technical scheme:

a functional single-atom coating for preventing and controlling African swine fever virus comprises the following components in percentage by weight: 15-25% of acrylic ester emulsion, 1-2% of defoaming agent, 2-5% of dispersing agent and 5-10% of propylene glycol. 1-2% of film-forming additive, 2-10% of thickening agent, 0.1-0.5% of pH regulator, 2-15% of single-atom catalyst for resisting African swine fever virus, 25-45% of filler powder and 15-35% of deionized water.

By adopting the technical scheme, the single-atom catalyst which is independently researched and developed and used for resisting the African swine fever virus is adopted, the African swine fever virus can be effectively killed, the African swine fever virus resisting rate is more than 99.9%, the continuous action time is long, and common pathogenic microorganisms in pig farms such as haemophilus parasuis, African swine fever, circovirus and blue ear virus can also be effectively killed. The coating prepared by the method can be specially used in livestock farms such as pig raising and the like, and can be conveniently coated on the surfaces of buildings such as a hog house and a delivery room, so that biological safety protection is effectively carried out on the African swine fever viruses.

Further, the product comprises the following components in percentage by weight: 18-22% of acrylate emulsion, 1-2% of defoaming agent, 2-5% of dispersing agent and 5-10% of propylene glycol. 1-2% of film-forming auxiliary agent, 4-8% of thickening agent, 0.2% of pH regulator, 5-10% of single-atom catalyst for resisting African swine fever virus, 30-40% of filler powder and 17-28% of deionized water; the acrylic ester emulsion is styrene-acrylic ester emulsion or acrylic emulsion for Acronal S400Fap flexible waterproof paint.

By adopting the technical scheme, because the coating is modified by the independently developed monoatomic antibacterial disinfectant, when the mass percent of the monoatomic antibacterial disinfectant is more than 5%, the antibacterial disinfection performance is not obviously increased along with the increase of the mass percent of the monoatomic antibacterial disinfectant, and 2-5% of the monoatomic antibacterial disinfectant can ensure the antibacterial disinfection performance and reduce the production cost.

Further, the single-atom catalyst for resisting the African swine fever virus consists of a carrier and an acetylacetone salt; the monatomic catalyst transition metal of the African swine fever virus resistance is anchored in a monatomic form in a defect site on the surface of the carrier; the mass ratio of the transition metal to the carrier in the single-atom catalyst for resisting African swine fever virus is 1:20-1:200

By adopting the technical scheme, the effective components of the transition metal for playing the role of antibacterial disinfection are controlled, when the mass ratio of the transition metal to the carrier is 1:20-1:200, the transition metal can be uniformly mixed with the filler when applied to the interior wall coating, so that the monoatomic antibacterial disinfectant can be separated out and released in the long-term use process, the quality of the produced monoatomic antibacterial disinfectant is ensured, the mass percentage of the monoatomic antibacterial disinfectant in the coating formula can be reduced, and the industrial production cost is reduced.

Further, the carrier is one or more of nano alumina and nano silica.

Further, the acetylacetone salt is selected from one or more of iron acetylacetonate, copper acetylacetonate, zinc acetylacetonate and silver acetylacetonate.

Further, the preparation method of the single-atom catalyst for resisting the African swine fever virus comprises the following steps:

s1, preparing a carrier precursor;

s2, preparing a metal monoatomic precursor;

s3, preparing a monoatomic catalyst precursor, adding the carrier precursor prepared in the step one into the mixed solution prepared in the step two, performing ultrasonic treatment, stirring and mixing, filtering, drying, and grinding the product to prepare powder;

s4, generating the monatomic catalyst in situ.

By adopting the technical scheme, the single-atom catalyst for killing the African swine fever virus and resisting the African swine fever virus can be prepared.

Further, the preparation method of the African swine fever virus resistant monatomic catalyst comprises the following steps:

s1, preparing a carrier precursor: taking nano-alumina and/or nano-silica as a raw material, adding a sodium carbonate solution with pH of 8, wherein the mass ratio of the raw material to an alkaline solution is 50:1, stirring and mixing uniformly, transferring the mixture into a reaction kettle for roasting and puffing, wherein the roasting temperature is 500 ℃, the pressure in the kettle is 0.8-1.0 Mpa, the roasting time is 10min, taking out the mixture, cooling the mixture, and grinding the mixture to obtain a carrier precursor;

s2, preparing a metal monoatomic precursor: dripping 10-30 mL of 5% ammonia water solution into 50-200 g/L acetylacetone salt solution at the speed of 100 mu L/s, stirring for 2-5 h, then heating to 60 ℃ within 20-40 min, and continuously stirring for 2-4 h to obtain a mixed solution;

s3, preparing a monoatomic catalyst precursor, adding the carrier precursor prepared in the step one into the step two, wherein the mass ratio of the transition metal to the carrier is 1:20-1:200, performing ultrasonic treatment on the prepared mixed solution for 30-60min, stirring for 12 hours, filtering, drying, and grinding the product to obtain the monoatomic antibacterial and antiviral catalyst with the required granularity of 1 micrometer;

s4, in situ generation of monatomic catalyst: and (3) heating the powder obtained in the third step at the temperature of 400-600 ℃ for 2-3h in a 5% hydrogen-argon mixed gas atmosphere, cooling and grinding to obtain the needed monoatomic antibacterial antiviral catalyst.

By adopting the technical scheme, the preparation parameters are accurately controlled, a stable and safe preparation method is obtained, and the single-atom catalyst capable of killing the African swine fever virus and resisting the African swine fever virus can be prepared.

Further, the filler powder comprises 6-10% of nano-grade rutile titanium dioxide, 6-10% of 6000-mesh kaolin, 6-8% of talcum powder and 7-14% of 4000-mesh nano calcium carbonate; the defoaming agent is a non-silicone mineral oil defoaming agent; the dispersant is 20% of polycarboxylic acid ammonium salt; the film-forming additive is alcohol ester twelve; the thickening agent is cellulose; the pH regulator is dimethylethanolamine.

By adopting the technical scheme, the good dispersion effect on the filler powder is obtained due to the adoption of the polycarboxylic acid ammonium salt; due to the adoption of cellulose, the effect of adjusting the viscosity and assisting in improving the antibacterial capacity of the coating is obtained; dimethyl ethanolamine is adopted to adjust the pH value and simultaneously used as a water-soluble paint cosolvent; the alcohol ester twelve can effectively reduce the lowest film-forming temperature, and is convenient for coating use. The use of the titanium dioxide can ensure that the interior wall coating is resistant to sunlight, does not crack or change color, has high extensibility and is acid and alkali resistant under the irradiation of sunlight; the kaolin has good bonding property, so that titanium dioxide, talcum powder and calcium carbonate can be better mixed together, and the mixing uniformity of the filler is ensured; the talcum powder has good covering power and lubricating effect; the calcium carbonate can prevent the paint from settling, so that the system is easy to disperse, and the prepared paint layer has good gloss, cold resistance and heat resistance.

In a second aspect, the application provides a preparation method of a functional monoatomic coating for preventing and controlling African swine fever virus, which adopts the following technical scheme:

step 1: adding deionized water into a container, sequentially adding a dispersing agent, a defoaming agent and propylene glycol under the stirring state of 300-;

step 2: adjusting the stirring speed to 500-600 revolutions per minute, sequentially adding filler powder and the monatomic catalyst for resisting the African swine fever virus, and stirring for 10-15 minutes;

and step 3: adding a thickening agent, adjusting the stirring speed to be 1000-;

and 4, step 4: and keeping the stirring rotation speed at 1000-1200 rpm, sequentially adding the acrylate emulsion, the film-forming assistant, the defoaming agent and part of deionized water, continuously stirring for 10 minutes, adjusting the stirring rotation speed to gradually reduce the rotation speed, adding the defoaming agent, and continuously stirring for 30 minutes to finally obtain the finished coating.

By adopting the technical scheme, the preparation method perfectly integrates the monatomic catalyst for resisting the African swine fever virus into the coating system, has a good killing effect on the African swine fever virus, is simple to operate, and can be used for large-scale industrial production.

Further, in the step 4, the acrylate emulsion, the film-forming assistant, the defoaming agent and the deionized water are sequentially added, the high-speed dispersion is continued for 10 minutes, the initial stirring speed is 1000-1200 rpm, the stirring speed is reduced by 100-150 rpm, the stirring speed is adjusted to 300 rpm, the defoaming agent is added, the stirring is continued for 30 minutes, and finally the coating finished product is prepared.

By adopting the technical scheme, the acrylic emulsion, the film-forming assistant, the defoaming agent and the antibacterial disinfectant in the step 4 are further uniformly mixed, and the quality of the prepared interior wall coating is ensured.

In conclusion, the invention has the following beneficial effects:

1. the special functional monoatomic coating for resisting the African swine fever virus uses a single-atom catalyst for resisting the African swine fever virus, which is independently developed, and adopts a completely new monoatomic catalysis technology, so that the African swine fever virus can be effectively killed, the African swine fever virus resistance rate is more than 99.9 percent, the sustained action time is long, and meanwhile, common pathogenic microorganisms in pig farms with haemophilus parasuis, African swine fever, circovirus and blue ear virus can be effectively killed.

2. The application perfectly fuses the advanced single-atom catalyst for resisting the African swine fever virus and the coating system, and develops a brand-new special functional single-atom coating with high-efficiency and durable resistance to the African swine fever virus. The coating can be specially used in livestock farms such as pig raising and the like, and can be conveniently coated on the surfaces of various buildings such as a pigsty, a delivery room and the like, so that biological safety protection is effectively carried out on African swine fever viruses.

3. The preparation method has the effects of simple preparation operation of the coating and large-scale industrial production.

Drawings

FIG. 1 is a graph showing the cell infection of African swine fever virus liquid on a paint plate at different times when the paint prepared in example 1 is used for anti-African swine fever virus experimental tests.

FIG. 2 is a graph showing the cell infection of African swine fever virus liquid on a paint plate at different times when the coating prepared in example 1 was subjected to repeated experimental tests for anti-African swine fever virus.

FIG. 3 is a transmission electron microscope image corrected for spherical aberration of the monoatomic catalyst against African swine fever virus prepared in preparation example 3.

Detailed Description

The present invention will be described in further detail with reference to examples.

Raw materials

Preparation examples of raw materials

Preparation example 1

The preparation of the single-atom antibacterial antiviral catalyst comprises the following steps:

step 1, preparing a carrier precursor: adding 2g of sodium carbonate solution with PH 8 into 100g of nano silicon oxide with the particle size of 500, stirring and mixing at 450rpm for 120min, transferring the mixture into a reaction kettle for roasting and puffing, wherein the roasting temperature is 500 ℃, the pressure in the kettle is 0.8MPa, and the roasting time is 10min, taking out the reaction kettle after roasting and sintering, cooling to room temperature, and grinding a product by using a fixed star ball mill (polytetrafluoroethylene is used as an inner container and zirconium oxide grinding balls with the particle size of 1.2 mm) to prepare a carrier precursor with the particle size of 500 nm;

step 2, preparing a metal monoatomic precursor: dripping 10ml of 5% ammonia water solution into 100ml of 50g/L ferric acetylacetonate and 100g/L copper acetylacetonate aqueous solution at the speed of 100 mu L/s, wherein the molar ratio of iron to copper is 1:1, stirring for 3h, then heating to 60 ℃ within 30min, continuing stirring for 3h, and cooling to room temperature after stirring to obtain a mixed solution;

step 3, preparing a monatomic catalyst precursor: adding the carrier precursor prepared in the step (1) into the mixed solution prepared in the step (2) according to the mass ratio of the transition metal to the carrier of 1:20, carrying out ultrasonic stirring for 30min, then carrying out stirring and mixing for 12h at the stirring speed of 450rpm, filtering, drying, and grinding a product by using a fixed star ball mill (polytetrafluoroethylene is used as an inner container and zirconia grinding balls with the diameter of 1.2 mm) for 30min to prepare powder;

step 4, in-situ generation of a monatomic catalyst: heating the obtained powder in 5% hydrogen-argon mixed gas atmosphere at 400 deg.C for 2h, cooling to room temperature, grinding the product with star ball mill (polytetrafluoroethylene as inner container, 1.2mm zirconium oxide grinding ball) to obtain monatomic catalyst with desired particle diameter of 1 μm, and bonding active metal contained in the obtained catalyst on the carrier in monatomic form.

Preparation example 2

step 1, preparing a carrier precursor: adding 2g of sodium carbonate solution with PH 8 into 100g of nano silicon oxide with the particle size of 8, stirring and mixing at 600rpm for 60min, then transferring to a reaction kettle for roasting and puffing, wherein the roasting temperature is 500 ℃, the pressure in the kettle is 0.9MPa, and the roasting time is 10min, after roasting and sintering, taking out the reaction kettle and cooling to room temperature, and grinding a product by using a fixed star ball mill (polytetrafluoroethylene is used as an inner container and zirconium oxide grinding balls with the particle size of 1.2 mm) to prepare a carrier precursor with the particle size of 500 nm;

step 2, preparing a metal monoatomic precursor: dropwise adding 20ml of 5% ammonia water solution into 200ml of 200g/L manganese acetylacetonate and 100g/L zinc acetylacetonate aqueous solution at the speed of 100 mu L/s, stirring for 3h, wherein the molar ratio of manganese to copper is 1:1, then heating to 60 ℃ within 30min, continuing stirring for 3h, and cooling to room temperature after stirring to obtain a mixed solution;

step 3, preparing a monatomic catalyst precursor: adding the carrier precursor prepared in the step (1) into the mixed solution prepared in the step (2) according to the mass ratio of the transition metal to the carrier of 1:20, carrying out ultrasonic stirring for 30min, then carrying out stirring and mixing for 12h at the stirring speed of 200rpm, filtering, drying, and grinding a product by using a fixed star ball mill (polytetrafluoroethylene is used as an inner container and zirconia grinding balls with the diameter of 1.2 mm) for 30min to prepare powder;

step 4, in-situ generation of a monatomic catalyst: heating the obtained powder in 5% hydrogen-argon mixed gas atmosphere at 500 deg.C for 2h, cooling to room temperature, grinding the product with star ball mill (polytetrafluoroethylene as inner container, 1.2mm zirconia grinding ball) to obtain monatomic catalyst with desired particle size of 1 micrometer, and bonding the active metal contained in the prepared catalyst on the carrier in monatomic form.

Preparation example 3

step 1, preparing a carrier precursor: adding 2g of sodium carbonate solution with PH (8) into 100g of nano silicon oxide with the particle size of 500, stirring and mixing at 750rpm for 80min, transferring the mixture into a reaction kettle for roasting and puffing, wherein the roasting temperature is 500 ℃, the pressure in the kettle is 1.0MPa, and the roasting time is 10min, taking out the reaction kettle after roasting and sintering, cooling to room temperature, grinding a product by using a fixed star ball mill (polytetrafluoroethylene is used as an inner container and zirconia grinding balls with the particle size of 1.2 mm) to prepare a carrier precursor with the particle size of 500nm for 30 min;

step 2, preparing a metal monoatomic precursor: dropwise adding 30ml of 5% ammonia water solution into 150ml of 50g/L ferric acetylacetonate, 50g/L copper acetylacetonate and 50g/L silver acetylacetonate aqueous solution at the speed of 100 mu L/s, stirring for 3h, wherein the molar ratio of iron to copper to silver is 50:50:1, then heating to 60 ℃ within 30min, continuing stirring for 3h, and cooling to room temperature after stirring to obtain a mixed solution;

step 3, preparing a monatomic catalyst precursor: adding the carrier precursor prepared in the step (1) into the mixed solution prepared in the step (2) according to the mass ratio of the transition metal to the carrier of 1:20, carrying out ultrasonic stirring for 30min, then carrying out stirring and mixing for 12h at the stirring speed of 350rpm, filtering, drying, and grinding a product by using a fixed star ball mill (polytetrafluoroethylene is used as an inner container and zirconia grinding balls with the diameter of 1.2 mm) for 30min to prepare powder;

step 4, in-situ generation of a monatomic catalyst: the obtained powder was heat-treated in a 5% hydrogen-argon atmosphere at 600 ℃ for 2 hours, cooled to room temperature, and the resultant product was ground with a star ball mill (polytetrafluoroethylene as an inner container, 1.2mm zirconia balls) to obtain a monatomic catalyst having a desired particle diameter of 1 μm, and the active metal contained in the catalyst was bound in a monatomic form to the carrier, as shown in FIG. 3.

Examples

Example one

A functional single-atom coating for preventing and controlling African swine fever virus comprises the following components in percentage by weight: 18 percent of styrene-acrylate emulsion, 1 percent of non-silicone mineral oil defoaming agent and sodium polycarboxylate

(20%) 2%, propylene glycol 5%, Loxanol CA 53081%, Cellosize hydroxyethyl cellulose 4%, Vantex-T0.2%, African swine fever virus-resistant monatomic catalyst 2% in preparation example 1, filler powder 40%, deionized water 26.8%, filler powder comprising 10% by mass of the total raw material of nano-rutile titanium dioxide (Ningbo Mingnan New materials science and technology Co., Ltd.), 10% of 6000-mesh kaolin (Shijiazhuang Asahi mineral products processing Co., Ltd.), 6% of 6000-mesh talc (Shijiazhuang Asahi mineral products processing Co., Ltd.), 14% of 4000-mesh nano-calcium carbonate (Guangdong source Lei Co., Ltd.), and a catalyst for resisting African swine fever virusPowder limited).

A preparation method of a functional monoatomic coating for preventing and controlling African swine fever virus comprises the following steps:

1) adding 24.8kg of deionized water into a stainless steel batching barrel, adopting mechanical stirring, stirring at the rotating speed of 300 r/min, and sequentially adding 2kg of 20% of sodium polycarboxylate salt under the stirring state

0.3kg of non-silicone mineral oil defoamer and 5kg of propylene glycol were stirred for 5 minutes to uniformly mix the materials added into the stainless steel batching barrel.

2) The stirring speed is adjusted to 500 revolutions per minute, 10kg of nano-grade rutile titanium dioxide, 10kg of 6000-mesh kaolin, 6kg of talcum powder, 14kg of 4000-mesh nano calcium carbonate and 2kg of the monatomic catalyst for resisting the African swine fever virus in the preparation example 1 are sequentially added into a stainless steel batching barrel at the feeding speed of 1.5 kg/minute, and the materials are fully stirred for 15 minutes.

3) 4.0kg of Cellosize hydroxyethylcellulose from Dow USA was added to a stainless steel batching drum at a feed rate of 0.20 kg/min, the speed was adjusted to 1000 rpm, stirring was continued for 30 minutes, followed by the addition of 0.2kg of Vantex-T, adjusted to a pH in the range 6.5-7.

4) Under the condition of keeping 1000 revolutions per minute, adding 19kg of styrene-acrylate emulsion, 1kg of Loxanol CA 5308, 0.3kg of non-silicone mineral oil defoaming agent and 2kg of deionized water in sequence, regulating the rotating speed to 1200 revolutions per minute, continuously dispersing for 10 minutes to completely and uniformly mix all materials, gradually reducing the rotating speed to 300 revolutions per minute at the speed of reducing 150 revolutions per minute, adding 0.4kg of defoaming agent, continuously stirring for 30 minutes, and finally obtaining a coating finished product.

Example two

A functional single-atom coating for efficiently preventing and controlling African swine fever viruses comprises the following components in percentage by weight: 20% of styrene-acrylate emulsion, 1.3% of defoaming agent, 3% of dispersing agent, 6.5% of propylene glycol, 1.3% of film-forming additive, 5.5% of thickening agent, 0.2% of pH regulator, 3% of monatomic catalyst for resisting African swine fever virus in preparation example 1, 36% of filler powder, 23.2% of deionized water, wherein the filler powder is composed of 9% of nano-grade rutile titanium dioxide, 9% of 6000-mesh kaolin, 5% of 6000-mesh talcum powder and 13% of 4000-mesh nano calcium carbonate.

A preparation method of a functional monoatomic coating for efficiently preventing and controlling African swine fever virus comprises the following steps:

1) adding 21.2kg of deionized water into a stainless steel batching barrel, adopting mechanical stirring, stirring at the rotating speed of 300 r/min, and sequentially adding 3.0kg of 20% sodium polycarboxylate in the stirring state

0.4kg of non-silicone mineral oil defoamer and 6.5kg of propylene glycol were stirred for 5 minutes to mix the materials added to the stainless steel batching barrel uniformly.

2) The stirring speed is adjusted to 500 revolutions per minute, 9kg of nano-grade rutile titanium dioxide, 9kg of 6000-mesh kaolin, 5kg of talcum powder, 13kg of 4000-mesh nano calcium carbonate and 3kg of the monatomic catalyst for resisting the African swine fever virus in the preparation example 1 are sequentially added into a stainless steel batching barrel at the feeding speed of 1.5 kg/minute, and the materials are fully stirred for 15 minutes.

3) 5.5kg of Cellosize hydroxyethylcellulose from Dow USA was added to a stainless steel batching drum at a feed rate of 0.20 kg/min, the speed was adjusted to 1000 rpm, stirring was continued for 30 minutes, followed by 0.2kg of Vantex-T, adjusted to a pH in the range 6.5-7.

4) Under the condition of keeping 1000 revolutions per minute, sequentially adding 20kg of styrene-acrylate emulsion, 1.3kg of Loxanol CA 5308, 0.4kg of non-silicone mineral oil defoaming agent and 2kg of deionized water, regulating the rotating speed to 1200 revolutions per minute, continuously dispersing for 10 minutes to completely and uniformly mix all materials, gradually reducing the rotating speed to 300 revolutions per minute at the speed of reducing 150 revolutions per minute, adding 0.5kg of defoaming agent, continuously stirring for 30 minutes, and finally obtaining a coating finished product.

EXAMPLE III

The difference between the third embodiment and the first embodiment is that: a functional single-atom coating for efficiently preventing and controlling African swine fever virus comprises the following components in percentage by weight: 22% of styrene-acrylate emulsion, 1.6% of defoaming agent, 4% of dispersing agent, 8% of propylene glycol, 1.6% of film-forming additive, 7% of thickening agent, 0.2% of pH regulator, 4% of single-atom catalyst for resisting African swine fever virus in preparation example 1, 33% of filler powder, 18.6% of deionized water, and the filler powder consists of 8% of nano-grade rutile titanium dioxide, 8% of 6000-mesh kaolin, 7% of 6000-mesh talcum powder and 10% of 4000-mesh nano calcium carbonate.

1) 16.6kg of deionized water is added into a stainless steel batching barrel, mechanical stirring is adopted, the stirring speed is 300 r/min, and 4.0kg of 20% sodium polycarboxylate is sequentially added under the stirring state

0.5kg of non-silicone mineral oil defoamer and 8kg of propylene glycol were stirred for 5 minutes to uniformly mix the materials added into the stainless steel batching barrel.

2) The stirring speed is adjusted to 500 revolutions per minute, 8kg of nano-grade rutile titanium dioxide, 8kg of 6000-mesh kaolin, 7kg of talcum powder, 10kg of 4000-mesh nano calcium carbonate and 4kg of the monatomic catalyst for resisting the African swine fever virus in the preparation example 1 are sequentially added into a stainless steel batching barrel at the feeding speed of 1.6kg per minute, and the materials are fully stirred for 15 minutes.

3) 7.0kg of Cellosize hydroxyethylcellulose from Dow USA was added to a stainless steel batching drum at a feed rate of 0.25 kg/min, the speed was adjusted to 1000 rpm, stirring was continued for 30 minutes, followed by 0.2kg of Vantex-T, adjusted to a pH in the range of 6.5-7.

4) Under the condition of keeping 1000 revolutions per minute, sequentially adding 22kg of styrene-acrylate emulsion, 1.6kg of Loxanol CA 5308, 0.5kg of non-silicone mineral oil defoaming agent and 2kg of deionized water, regulating the rotating speed to 1200 revolutions per minute, continuously dispersing for 10 minutes to completely and uniformly mix all materials, gradually reducing the rotating speed to 300 revolutions per minute at the speed of reducing 150 revolutions per minute, adding 0.6kg of defoaming agent, continuously stirring for 30 minutes, and finally obtaining a coating finished product.

Example four

The difference between the fourth embodiment and the first embodiment is that: 20% of styrene-acrylate emulsion, 2% of defoaming agent, 5% of dispersing agent, 10% of propylene glycol, 2% of film-forming additive, 8% of thickening agent, 0.2% of pH regulator, 5% of single-atom catalyst for resisting African swine fever virus in preparation example 1, 30% of filler powder and 17.8% of deionized water. The filler powder consists of 7 percent of nano rutile titanium dioxide, 7 percent of 6000-mesh kaolin, 8 percent of 6000-mesh talcum powder and 8 percent of 4000-mesh nano calcium carbonate.

1) adding 15.8kg of deionized water into a stainless steel batching barrel, adopting mechanical stirring, stirring at the rotating speed of 300 r/min, and sequentially adding 5.0kg of 20% sodium polycarboxylate in the stirring state

0.6kg of non-silicone mineral oil defoamer and 10kg of propylene glycol were stirred for 5 minutes to uniformly mix the materials added into the stainless steel batching barrel.

2) The stirring speed is adjusted to 500 revolutions per minute, 7kg of nano-grade rutile titanium dioxide, 7kg of 6000-mesh kaolin, 8kg of talcum powder, 8kg of 4000-mesh nano calcium carbonate and 5kg of the monatomic catalyst for resisting the African swine fever virus in the preparation example 1 are sequentially added into a stainless steel batching barrel at the feeding speed of 1.25 kg/minute, and the materials are fully stirred for 15 minutes.

3) 8.0kg of Cellosize hydroxyethylcellulose from Dow USA was added to a stainless steel batching drum at a feed rate of 0.25 kg/min, the speed was adjusted to 1000 rpm, stirring was continued for 30 minutes, followed by 0.2kg of Vantex-T, adjusted to a pH in the range of 6.5-7.

4) Under the condition of keeping 1000 revolutions per minute, sequentially adding 20kg of styrene-acrylate emulsion, 2.0kg of Loxanol CA 5308, 0.6kg of non-silicone mineral oil defoaming agent and 2kg of deionized water, regulating the rotating speed to 1200 revolutions per minute, continuously dispersing for 10 minutes to completely and uniformly mix all materials, gradually reducing the rotating speed to 300 revolutions per minute at the speed of reducing 150 revolutions per minute, adding 0.8kg of defoaming agent, continuously stirring for 30 minutes, and finally obtaining a coating finished product.

EXAMPLE five

The difference between the fifth embodiment and the first embodiment is that: the single-atom catalyst for resisting African swine fever virus in preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in preparation example 2.

EXAMPLE six

The difference between the sixth embodiment and the second embodiment is that: the single-atom catalyst for resisting African swine fever virus in preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in preparation example 2.

EXAMPLE seven

The difference between the seventh embodiment and the third embodiment is that: the single-atom catalyst for resisting African swine fever virus in preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in preparation example 2.

Example eight

The difference between the eighth embodiment and the fourth embodiment is that: the single-atom catalyst for resisting African swine fever virus in preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in preparation example 2.

Example nine

The difference between the ninth embodiment and the first embodiment is that: the single-atom catalyst for resisting African swine fever virus in preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in preparation example 3.

Example ten

The difference between the tenth embodiment and the second embodiment is that: the single-atom catalyst for resisting African swine fever virus in preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in preparation example 3.

EXAMPLE eleven

The difference between the eleventh embodiment and the third embodiment is that: the single-atom catalyst for resisting African swine fever virus in the preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in the preparation example 3.

Example twelve

The difference between the twelfth embodiment and the fourth embodiment is that: the single-atom catalyst for resisting African swine fever virus in the preparation example 1 is replaced by the single-atom catalyst for resisting African swine fever virus in the preparation example 3.

EXAMPLE thirteen

The thirteenth embodiment differs from the first embodiment in that: the styrene-acrylate emulsion was replaced with an acrylic emulsion for Acronal S400F ap flexible waterproof paint.

Example fourteen

The fourteenth embodiment is different from the second embodiment in that: the styrene-acrylate emulsion was replaced with an acrylic emulsion for Acronal S400F ap flexible waterproof paint.

Example fifteen

The fifteenth embodiment differs from the third embodiment in that: the styrene-acrylate emulsion was replaced with an acrylic emulsion for Acronal S400F ap flexible waterproof paint.

Example sixteen

Example sixteen differs from example four in that: the styrene-acrylate emulsion was replaced with an acrylic emulsion for Acronal S400F ap flexible waterproof paint.

Comparative example

Comparative example 1

Comparative example 1 differs from example one in that: the single-atom catalyst for resisting African swine fever virus is not added, and other components are the same.

Comparative example 2

Comparative example 2 differs from example thirteen in that: the monoatomic catalyst for resisting African swine fever virus is not added, and other components are the same.

Performance test

anti-African swine fever virus experimental test:

step 1, mixing 900 mu L of culture solution and 100 mu L of African swine fever virus solution uniformly, taking 600 mu L of the mixture to a coating plate, placing the coating plate on an aseptic culture dish, and placing the coating plate at room temperature.

And 2, taking out 5 mu L of the cell culture medium respectively at 30min, 1h, 6h, 12h and 24h, adding the cell culture medium into a cell culture hole, and observing the state of the cell and whether the cell has fluorescence or not.

The experiment was repeated: 900 mul of culture solution and 100 mul of African swine fever virus solution are mixed evenly, 600 mul are taken to be put on a coating plate, and the coating plate is placed in a sterile culture dish. 400 μ L of African swine fever virus liquid remained as control. Standing at room temperature, taking out 5 μ L of the culture solution at 1h, mixing with 400 μ L of the cell culture solution, adding into cell culture wells, and changing the solution after 2 h. The treatment methods of 1h, 3h, 6h, 12h and 24h are the same as above. The cell state and the presence or absence of fluorescence of the cells were observed.

Detection method/test method

TABLE 1 Experimental test parameters for anti-African Swine fever Virus of examples 1-4 and comparative examples 1-2

TABLE 2 Experimental test parameters for anti-African Swine fever Virus of examples 5-8 and comparative examples 1-2

	30min	1h	6h	12h	24h
						Example five coated Panel	+	+	-	-	-
Control group of example five	+	+	+	+	+
						Example six coated Panel	+	+	-	-	-
Control of example six	+	+	+	+	+
						EXAMPLE seven coated Panel	+	+	-	-	-
EXAMPLE seven control group	+	+	+	+	+
						Example eight coated Panel	+	+	-	-	-
Example eight control groups	+	+	+	+	+
						Coating plate of comparative example one	+	+	+	+	+
Control group of comparative example one	+	+	+	+	+
						Coating Panel of comparative example No. two	+	+	+	+	+
Control group of comparative example II	+	+	+	+	+

TABLE 3 Experimental test parameters for anti-African Swine fever Virus of examples 9-12 and comparative examples 1-2

	30min	1h	6h	12h	24h
						EXAMPLE nine coated Panel	+	+	-	-	-
EXAMPLE nine control group	+	+	+	+	+
						Example ten coated Panel	+	+	-	-	-
Control group of example ten	+	+	+	+	+
						Example eleven A coated Panel	+	+	-	-	-
Control of EXAMPLE eleven	+	+	+	+	+
						Example twelve coated Panel	+	+	-	-	-
Control of EXAMPLE twelve	+	+	+	+	+
						Coating plate of comparative example one	+	+	+	+	+
Control group of comparative example one	+	+	+	+	+
						Coating Panel of comparative example No. two	+	+	+	+	+
Control group of comparative example II	+	+	+	+	+

TABLE 4 Experimental test parameters for anti-African Swine fever Virus of examples 13-16 and comparative examples 1-2

As can be seen by combining examples 1-4 and comparative examples 1-2 with Table 1 and FIG. 1, the control group African swine fever virus infected cells at 24 h. The African swine fever virus liquid (the pH value is changed from 7.2 to 8.5) on the coating plate has an influence on the cell state, a cell culture hole treated for 30min and 1h has little fluorescence, the cell state of 6h, 12h and 24h is poor, and no fluorescence is visible.

As can be seen by combining examples 5-8 and comparative examples 1-2 with Table 2, the control group of African swine fever virus infected cells at 24 h. The African swine fever virus liquid (the pH value is changed from 7.2 to 8.5) on the coating plate has an influence on the cell state, a cell culture hole which is processed for 30min and 1h has little fluorescence, the cell state of 6h, 12h and 24h is poor, and no fluorescence is visible.

As can be seen by combining examples 9-12 and comparative examples 1-2 with Table 3, the control group African swine fever virus infected cells at 24 h. The African swine fever virus liquid (the pH value is changed from 7.2 to 8.5) on the coating plate has an influence on the cell state, a cell culture hole which is processed for 30min and 1h has little fluorescence, the cell state of 6h, 12h and 24h is poor, and no fluorescence is visible.

In combination with examples 13 to 16 and comparative examples 1 to 2 and in combination with Table 4, it can be seen that the control group African swine fever virus infected cells at 24 h. The African swine fever virus liquid (the pH value is changed from 7.2 to 8.5) on the coating plate has an influence on the cell state, a cell culture hole which is processed for 30min and 1h has little fluorescence, the cell state of 6h, 12h and 24h is poor, and no fluorescence is visible. And the acrylic emulsion for the flexible waterproof coating adopting Acronal S400F ap can be better combined with cement to be coated and has better waterproofness.

TABLE 5 repeated experimental test parameters for anti-African swine fever virus of examples 1-4 and comparative examples 1-2

TABLE 6 repeated experimental test parameters for anti-African swine fever virus of examples 5-8 and comparative examples 1-2

	1h	3h	6h	12h	24h
						Example five coated Panel	+	+	-	-	-
Control group of example five	+	+	+	+	+
						Example six coated Panel	+	+	-	-	-
Control of example six	+	+	+	+	+
						EXAMPLE seven coated Panel	+	+	-	-	-
EXAMPLE seven control group	+	+	+	+	+
						Example eight coated Panel	+	+	-	-	-
Example eight control groups	+	+	+	+	+
						Coating plate of comparative example one	+	+	+	+	+
Control group of comparative example one	+	+	+	+	+
						Coating Panel of comparative example No. two	+	+	+	+	+
Control group of comparative example II	+	+	+	+	+

TABLE 7 repeated experimental test parameters for anti-African swine fever virus of examples 9-12 and comparative examples 1-2

	1h	3h	6h	12h	24h
						EXAMPLE nine coated Panel	+	+	-	-	-
EXAMPLE nine control group	+	+	+	+	+
						Example ten coated Panel	+	+	-	-	-
Control group of example ten	+	+	+	+	+
						Example eleven A coated Panel	+	+	-	-	-
Control of EXAMPLE eleven	+	+	+	+	+
						Example twelve coated Panel	+	+	-	-	-
Control of EXAMPLE twelve	+	+	+	+	+
						Coating plate of comparative example one	+	+	+	+	+
Control group of comparative example one	+	+	+	+	+
						Coating Panel of comparative example No. two	+	+	+	+	+
Control group of comparative example II	+	+	+	+	+

TABLE 8 repeated experimental test parameters for anti-African swine fever virus of examples 13-16 and comparative examples 1-2

	1h	3h	6h	12h	24h
						Example thirteen coated Panel	+	+	-	-	-
Control of EXAMPLE thirteen	+	+	+	+	+
						Example fourteen coated Panel	+	+	-	-	-
Example fourteen control group	+	+	+	+	+
						Example fifteen coated Panel	+	+	-	-	-
EXAMPLE fifteen control group	+	+	+	+	+
						EXAMPLE sixteen coated Panel	+	+	-	-	-
EXAMPLE sixteen control groups	+	+	+	+	+
						Coating plate of comparative example one	+	+	+	+	+
Control group of comparative example one	+	+	+	+	+
						Coating Panel of comparative example No. two	+	+	+	+	+
Control group of comparative example II	+	+	+	+	+

As can be seen by combining examples 1-16 and comparative examples 1-2 with tables 5-8 and FIG. 2, 106TCID50/ml of virus solution was effective in inactivating African swine fever virus when placed on a coated panel for 12 hours at room temperature.

In conclusion, the single-atom catalyst which is independently developed and developed to resist the African swine fever virus is perfectly fused with the coating system (namely, the addition of the single-atom catalyst does not damage the function of the coating and can be added into the coating system), and a brand-new special functional single-atom coating which has high efficiency and durability to resist the African swine fever virus is developed, can effectively kill the African swine fever virus, has the African swine fever virus resistance rate of more than 99.9 percent and has long lasting action time. The coating prepared by the method can be specially used in livestock farms such as pig raising and the like, and can be conveniently coated on the surfaces of various buildings such as a pigsty and a delivery room, so that the African swine fever virus can be effectively and safely protected.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.

Claims

1. The functional monoatomic coating for preventing and controlling African swine fever virus is characterized by comprising the following components in percentage by weight: 15-25% of acrylate emulsion, 1-2% of defoaming agent, 2-5% of dispersing agent, 5-10% of propylene glycol, 1-2% of film-forming additive, 2-10% of thickening agent, 0.1-0.5% of pH regulator, 2-15% of single-atom catalyst for resisting African swine fever virus, 25-45% of filler powder and 15-35% of deionized water.

2. The functional monoatomic coating for controlling African swine fever virus according to claim 1, wherein the product comprises the following components in percentage by weight: 18-22% of acrylate emulsion, 1-2% of defoaming agent, 2-5% of dispersing agent, 5-10% of propylene glycol, 1-2% of film-forming additive, 4-8% of thickening agent, 0.2% of pH regulator, 5-10% of single-atom catalyst for resisting African swine fever virus, 30-40% of filler powder and 17-28% of deionized water; the acrylic ester emulsion is styrene-acrylic ester emulsion or acrylic emulsion for Acronal S400Fap flexible waterproof paint.

3. The functional monatomic coating for controlling African swine fever virus according to claim 1, wherein the African swine fever virus-resistant monatomic catalyst consists of a carrier and an acetylacetonate; the transition metal of the African swine fever virus resistant monatomic catalyst is anchored in a defect site on the surface of the carrier in a monatomic form; the mass ratio of the transition metal to the carrier in the single-atom catalyst for resisting the African swine fever virus is 1:20-1: 200.

4. The functional monatomic coating for controlling african swine fever virus according to claim 3, wherein the carrier is one or more of nano alumina and nano silica.

5. The functional monoatomic coating for controlling African swine fever virus according to claim 3, wherein the acetylacetone salt is selected from one or more of iron acetylacetonate, copper acetylacetonate, zinc acetylacetonate, and silver acetylacetonate.

6. The functional monatomic coating for controlling African swine fever virus according to claim 1, wherein the preparation method of the African swine fever virus-resistant monatomic catalyst comprises the following steps:

s1, preparing a carrier precursor;

s2, preparing a metal monoatomic precursor;

s4, generating the monatomic catalyst in situ.

7. The functional monatomic coating for controlling African swine fever virus according to claim 6, wherein the preparation method of the African swine fever virus-resistant monatomic catalyst comprises the following steps:

s1, preparing a carrier precursor: taking nano alumina and/or nano silicon oxide as a raw material, adding a sodium carbonate solution with the pH =8, wherein the mass ratio of the raw material to an alkaline solution is 50:1, stirring and mixing uniformly, transferring the mixture into a reaction kettle for roasting and puffing, wherein the roasting temperature is 500 ℃, the pressure in the kettle is 0.8-1.0 Mpa, the roasting time is 10min, taking out the mixture, cooling the mixture, and grinding the mixture to obtain a carrier precursor;

s2, preparing a metal monoatomic precursor: dripping 10-30 mL of 5% ammonia water solution into 50-200 g/L acetylacetone salt solution at the speed of 80-120 mu L/s, stirring for 2-5 h, then heating to 60 ℃ within 20-40 min, and continuously stirring for 2-4 h to obtain a mixed solution;

s3, preparing a monoatomic catalyst precursor, adding the carrier precursor prepared in the step one into the step two, wherein the mass ratio of the transition metal to the carrier is 1:20-1:200, performing ultrasonic treatment on the prepared mixed solution for 30-60min, stirring for 12 hours, filtering, drying, and grinding the product to obtain powder;

s4, in situ generation of monatomic catalyst: and (3) heating the powder obtained in the third step for 2-3h at the temperature of 400-600 ℃ in a 5% hydrogen-argon mixed gas atmosphere, cooling and grinding to obtain the needed monoatomic antibacterial antiviral catalyst with the granularity of 1 micrometer.

8. The functional monatomic coating for preventing and controlling African swine fever virus according to claim 1, wherein the filler powder comprises 6-10% of nano-grade rutile titanium dioxide, 6-10% of 6000-mesh kaolin, 6-8% of talcum powder and 7-14% of 4000-mesh nano calcium carbonate; the defoaming agent is a non-silicone mineral oil defoaming agent; the dispersant is 20% of polycarboxylic acid ammonium salt; the film-forming additive is alcohol ester twelve; the thickening agent is cellulose; the pH regulator is dimethylethanolamine.

9. The preparation method of the functional monoatomic coating for preventing and controlling African swine fever virus according to any one of claims 1-8, which comprises the following steps:

step 1: adding deionized water into a container, sequentially adding a dispersing agent, a defoaming agent and propylene glycol under the stirring state of 300-500 r/min, and stirring for 3-5 min;

and 4, step 4: and keeping the stirring speed at 1000-1200 rpm, sequentially adding the acrylate emulsion, the film-forming assistant, the defoaming agent and part of deionized water, continuously stirring for 10 minutes, adjusting the stirring speed to gradually reduce the speed and add the defoaming agent, and continuously stirring for 30 minutes to finally obtain the finished coating.

10. The method for preparing the functional monatomic coating for preventing and controlling African swine fever virus of claim 9, wherein in the step 4, the acrylate emulsion, the film-forming assistant, the antifoaming agent and the deionized water are sequentially added, the dispersion is continued for 10 minutes at a high speed, the initial stirring speed is 1000-1200 rpm, the stirring speed is reduced by 100-150 rpm, the stirring speed is adjusted to 300 rpm, the antifoaming agent is added, the stirring is continued for 30 minutes, and finally the coating finished product is prepared.