CN113064269A - Microscope and intelligent microscope system - Google Patents
Microscope and intelligent microscope system Download PDFInfo
- Publication number
- CN113064269A CN113064269A CN202110382334.XA CN202110382334A CN113064269A CN 113064269 A CN113064269 A CN 113064269A CN 202110382334 A CN202110382334 A CN 202110382334A CN 113064269 A CN113064269 A CN 113064269A
- Authority
- CN
- China
- Prior art keywords
- lens barrel
- motor
- controlling
- microscope
- axis motor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000007246 mechanism Effects 0.000 claims abstract description 35
- 238000001000 micrograph Methods 0.000 claims abstract description 8
- 239000011521 glass Substances 0.000 claims abstract description 4
- 238000004458 analytical method Methods 0.000 claims description 15
- 230000008878 coupling Effects 0.000 claims description 15
- 238000010168 coupling process Methods 0.000 claims description 15
- 238000005859 coupling reaction Methods 0.000 claims description 15
- 238000013473 artificial intelligence Methods 0.000 claims description 14
- 238000003708 edge detection Methods 0.000 claims description 4
- 230000000007 visual effect Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 9
- 230000003287 optical effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 241000463219 Epitheca Species 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/362—Mechanical details, e.g. mountings for the camera or image sensor, housings
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention discloses a microscope and an intelligent microscope system, the microscope comprises a bottom shell, an upper shell, an electric carrying mechanism, a light path mechanism and a lower computer, wherein the upper shell is positioned on the bottom shell, the upper shell and the bottom shell are both of a hollow structure and are mutually communicated, the upper surface of the bottom shell is provided with a window, the electric carrying mechanism is positioned in the bottom shell and can carry a slide and move in an X-axis plane and a Y-axis plane, the light path mechanism comprises a camera, a lens cone and an objective lens, the lens barrel is arranged along the Z axis and located above the window, the light source is located at the bottom of the bottom shell, the camera is located at the top end of the lens barrel, the objective lens is located at the bottom end of the lens barrel, the camera, the lens barrel, the objective lens, the glass slide and the light source form a light path, the Z axis motor is located in the upper shell and used for driving the lens barrel to move along the Z axis, and the lower machine is used for controlling the electric object carrying mechanism to move, controlling the Z axis motor to rotate and controlling the camera to shoot micrographs. The invention can automatically adjust the visual field and the automatic focusing, and improve the adjusting speed and the adjusting precision.
Description
Technical Field
The invention relates to the technical field of microscopes, in particular to a microscope and an intelligent microscope system.
Background
A microscope is an optical instrument consisting of one lens or a combination of several lenses and is mainly used to magnify tiny objects to be seen by the naked human eye. The traditional optical microscope needs manual field of vision adjustment and manual focusing, some full-automatic microscopes are also available on the market, the X-Y/Z three-axis control of an object stage and the automatic adjustment of the brightness of a light source can be realized, full-automatic functions such as panoramic automatic scanning, automatic picture splicing, automatic returning, synchronous browsing, remote control and the like are realized through software, the photoelectric computation integrated high-tech product with the purpose of improving the working efficiency is achieved, the improvement is basically based on the traditional microscope, and meanwhile, the price is higher than that of the traditional microscope.
Disclosure of Invention
The invention aims to provide a microscope and an intelligent microscope system, which can automatically adjust the visual field and automatically adjust the focus, and improve the adjusting speed and the adjusting precision.
In order to solve the technical problems, the invention adopts a technical scheme that: the utility model provides a microscope, includes drain pan, epitheca, electronic year thing mechanism, light path mechanism and next machine, the epitheca is located the drain pan, epitheca and drain pan are hollow structure and communicate each other, the window has been seted up to the upper surface of drain pan, electronic year thing mechanism is located the drain pan, can carry on the slide and move in X axle and Y axle plane, light path mechanism includes camera, lens cone, objective, Z axle motor and light source, the lens cone sets up and is located the window top along the Z axle, the light source is located the bottom of drain pan, the camera is located the top of lens cone, objective is located the bottom of lens cone, the light path is constituteed to camera, lens cone, objective, slide and light source, Z axle motor and next machine are located the epitheca, Z axle motor is used for driving the lens cone to remove along the Z axle, the next machine is used for controlling electronic year thing mechanism to remove, And controlling the Z-axis motor to rotate and controlling the camera to shoot the photomicrograph.
Preferably, the electric carrying mechanism comprises a Y-axis motor, an X-axis motor, a guide rail sliding table, a carrying table, a motor connector, a sliding connector, a first slide bar, a second slide bar and a lead screw, the guide rail sliding table is connected with a slide block in a sliding manner, the Y-axis motor is fixed on the guide rail sliding table to drive the slide block to slide along a first direction, the motor connector is fixed on the slide block, the first slide bar is fixed relative to the guide rail sliding table and is arranged along the first direction, the sliding connector is connected on the first slide bar in a sliding manner, two ends of the second slide bar are respectively fixed with the motor connector and the sliding connector, the lead screw and the second slide bar are parallel to each other and are arranged along a second direction vertical to the first direction, the X-axis motor is fixed on the motor connector and is connected with one end of the lead screw to drive the lead screw to, the second slide bar and the lead screw all pass the objective table, second slide bar and objective table sliding connection, lead screw and objective table threaded connection, the objective table is used for carrying glass slide, the next machine is used for controlling Y axle motor and X axle motor and rotates.
Preferably, the electric loading mechanism further comprises two fixing seats, and the two fixing seats are respectively located at two ends of the first sliding rod and fixedly connected with two ends of the first sliding rod.
Preferably, the fixing seat is provided with three screw holes along the Z-axis direction, each fixing seat is provided with three screws, wherein two screws penetrate through the bottom of the bottom shell and then are in threaded connection with the two screw holes of the fixing seat, and the other screw penetrates through the bottom of the bottom shell after being in threaded connection with the other screw hole of the fixing seat.
Preferably, the bottom of motor connector is equipped with the installing support, the installing support is located between slider and the guide rail slip table to be close to the tip of guide rail slip table, be equipped with first limit switch on the installing support.
Preferably, one side of the motor connecting piece facing the objective table is provided with a mounting hole, and a second limit switch is arranged in the mounting hole.
Preferably, the motor connector is provided with a motor accommodating groove, and the second motor is fixed in the motor accommodating groove.
In order to solve the technical problem, the invention adopts another technical scheme that: the lower computer is in wireless connection or wired connection with the client, the client is in remote connection with the server, the server is in remote connection with the client, and the lower computer is used for controlling the electric loading mechanism to move a slide to a window, controlling a Z-axis motor to rotate to complete focusing, controlling a camera to scan to obtain a micrograph and uploading the micrograph to the server;
the server is used for carrying out artificial intelligence recognition on the photomicrographs to obtain artificial intelligence analysis results, pushing the artificial intelligence analysis results to the expert system for artificial authentication to obtain artificial authentication analysis results, generating a conclusion report by combining the artificial intelligence analysis results and the artificial authentication analysis results, and sending the conclusion report to the user terminal.
Preferably, the specific step of controlling the Z-axis motor to rotate by the lower computer to complete focusing includes:
s1: controlling a Z-axis motor to drive a lens barrel to reach a focusing initial position, and taking a preset initial step length as a current step length;
s2: controlling a Z-axis motor to drive a lens barrel to move downwards in a stepping mode according to the current step length, controlling a camera to capture images and calculating the image definition after each movement until the lens barrel reaches an ultimate focusing position, wherein the ultimate focusing position is below a focusing starting position;
s3: selecting the position of the lens barrel when the image definition is maximum as a reference position;
s4: judging whether the current step length is smaller than a preset threshold value, if so, performing step S5, and if not, performing step S6;
s5: controlling a Z-axis motor to drive a lens barrel to reach a reference position, and finishing focusing;
s6: controlling a Z-axis motor to drive a lens barrel to reach a position above the reference position and away from the reference position by the current step length;
s7: replacing the current step length by half of the current step length;
s8: controlling a Z-axis motor to drive a lens barrel to move downwards in a stepping manner at the current step length;
s9: controlling a camera to capture an image and calculating the image definition after the lens barrel moves;
s10: and judging whether the image definition is greater than that of the last position of the lens barrel, if not, taking the last position of the lens barrel as a reference position, and performing the step S4 again, and if so, performing the step S8 again.
Preferably, the image definition is calculated by an edge detection Laplacian operator or a Roberts gradient function.
Different from the prior art, the invention has the beneficial effects that:
1. the structure is more compact, reduces the volume of the microscope, and enables the microscope to have certain portability.
2. The lens cone and the objective table are automatically adjusted by a motor, so that the microscope can carry out operations such as automatic movement, focusing, scanning, splicing and the like.
3. Through setting up the server side, the photomicrograph can be gathered to the server side, and complicated operations such as artificial intelligence discernment are located the server side, can reduce cost.
4. The server can carry out artificial intelligence discernment, can improve the intellectuality.
Drawings
Fig. 1 is a schematic front view of a microscope according to an embodiment of the present invention.
Fig. 2 is a left side schematic view of a microscope of an embodiment of the invention.
Fig. 3 is a schematic top view of a microscope in accordance with an embodiment of the invention.
FIG. 4 is an axial schematic view of a microscope in accordance with an embodiment of the invention.
Fig. 5 is a disassembled schematic view of a microscope of an embodiment of the present invention.
Fig. 6 is a schematic front view of the optical path structure of the microscope according to the embodiment of the present invention.
Fig. 7 is a perspective schematic view of an optical path structure of a microscope according to an embodiment of the present invention.
Fig. 8 is a schematic front view of an electric loading mechanism of a microscope according to an embodiment of the present invention.
Fig. 9 is a schematic top view of the motorized stage mechanism of the microscope according to the embodiment of the present invention.
Fig. 10 is an axial view of the motorized stage mechanism of the microscope according to the embodiment of the present invention.
Fig. 11 is a partially exploded view of the motorized stage mechanism of the microscope according to the embodiment of the present invention.
Fig. 12 is a schematic structural view of a motor coupling of a motorized stage mechanism of a microscope according to an embodiment of the present invention.
Fig. 13 is a front view schematically illustrating a holder of a motorized stage mechanism of a microscope according to an embodiment of the present invention.
Fig. 14 is a perspective view of a holder of an electric stage mechanism of a microscope according to an embodiment of the present invention.
Fig. 15 is a perspective view schematically illustrating a holder of a motorized stage mechanism of a microscope according to an embodiment of the present invention.
Fig. 16 is a schematic composition diagram of an intelligent microscope system according to an embodiment of the invention.
Fig. 17 is a schematic flow chart of the lower computer controlling the Z-axis motor to rotate to complete focusing.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 7, a microscope according to an embodiment of the present invention includes a bottom case 1, an upper case 2, an electric object carrying mechanism, an optical path mechanism and a lower computer (not shown), wherein the upper case 2 is located on the bottom case 1, the upper case 2 and the bottom case 1 are both hollow and are communicated with each other, a window 11 is formed on an upper surface of the bottom case 1, the electric object carrying mechanism is located in the bottom case 1 and can carry a slide 46 and move in an X-axis and Y-axis plane, the optical path mechanism includes a camera 41, a lens barrel 42, an objective lens 43, a Z-axis motor 44 and a light source 45, the lens barrel 42 is arranged along a Z-axis and is located above the window 11, the light source 45 is located at a bottom of the bottom case 1, the camera 41 is located at a top end of the lens barrel 42, the objective lens 43 is located at a bottom end of the lens barrel 42, the objective lens 43, the slide 46 and the light source 45, the lower computer is used for controlling the electric loading mechanism to move, controlling the Z-axis motor 44 to rotate and controlling the camera 41 to shoot a micrograph. The lower machine may be located within the bottom shell 1 or the upper shell 2.
As shown in fig. 8 to 11, in the present embodiment, the electric loading mechanism includes a Y-axis motor 31, an X-axis motor 32, a rail slide table 33, a loading table 34, a motor coupling 35, a slide coupling 36, a first slide bar 37, a second slide bar 38, and a lead screw 39. A slide block 331 is connected on the guide rail sliding table 33 in a sliding manner, the Y-axis motor 31 is fixed on the guide rail sliding table 33 to drive the slide block 331 to slide along a first direction, the motor coupling 35 is fixed on the slide block 331, the first slide bar 37 is fixed relative to the guide rail sliding table 33 and arranged along the first direction, the sliding coupling 36 is connected on the first slide bar 37 in a sliding manner, two ends of the second slide bar 38 are respectively fixed with the motor coupling 35 and the sliding coupling 36, the screw rod 39 and the second slide bar 38 are parallel to each other and arranged along a second direction perpendicular to the first direction, the X-axis motor 32 is fixed on the motor coupling 35 and coupled with one end of the screw rod 39 to drive the screw rod 39 to rotate, the other end of the screw rod 39 is connected with the sliding coupling 36 in a rotating manner, the second slide bar 38 and the screw rod 39 both pass through the object stage 34, the second slide bar 38 is connected with the object stage 34 in, the lower computer is used for controlling the rotation of the Y-axis motor 31 and the X-axis motor 32.
As shown in fig. 9, the first direction and the second direction constitute Y-axis and X-axis of a planar coordinate system. When the Y-axis position of the object stage 34 needs to be adjusted, the first motor 31 drives the sliding block 331 to slide, thereby adjusting the position of the object stage 34 on the Y-axis. When the position of the object stage 34 on the X axis needs to be adjusted, the second motor 32 drives the screw rod 39 to rotate, and the object stage 34 moves on the screw rod 39 because the screw rod 39 is fixed relative to the object stage 34, so as to adjust the position of the object stage 34 on the X axis
As shown in fig. 13 to 15, the electric loading mechanism further includes two fixing bases 371, and the two fixing bases 371 are respectively located at two ends of the first sliding rod 37 and fixedly connected to two ends of the first sliding rod 37. The fixing seat 371 can play a role of fixing the first sliding bar 3.
Furthermore, three screw holes 372 are formed in the fixing seat 371 along the Z-axis direction, and three screws 373 are arranged on each fixing seat 371, wherein two screws 373 are connected with the two screw holes 372 of the fixing seat 371 through threads after passing through the bottom of the bottom case 1, and the other screw 373 is connected with the other screw hole 372 of the fixing seat 371 through threads and then passes through the bottom of the bottom case 1.
By means of the three screws 373, the horizontal height of the first slide bar 37 can be adjusted. Specifically, two screws 373 screwed in the same direction are used as adjusting screws (i.e., screws screwed from below in fig. 15), and the other screw 373 is used as a fixing screw (i.e., a screw screwed from above in fig. 15), the fixing screws are loosened, the horizontal height of the first slide bar 37 is adjusted by the adjusting screws, and after the adjustment is completed, the fixing screws are tightened to fix the horizontal height of the first slide bar 37.
In order to prevent the sliding block 331 from mechanical collision during movement, in this embodiment, the bottom end of the motor coupling member 35 is provided with a mounting bracket 351, the mounting bracket 351 is located between the sliding block 331 and the guide rail sliding table 33 and is close to the end of the guide rail sliding table 33, and the mounting bracket 351 is provided with a first limit switch (not shown).
Further, in order to prevent the object stage 34 from mechanical collision during movement, in the present embodiment, a mounting hole 352 is provided on a side of the motor coupling 35 facing the object stage 34, and a second limit switch (not shown) is provided in the mounting hole 352.
The first limit switch and the second limit switch are, for example, touch switches, and when the slide block 331 or the object stage 34 touches the touch switches to cause the touch switches to perform a switching operation, the Y-axis motor 31 or the X-axis motor 32 receives a corresponding signal, and stops driving, and the slide block 331 or the object stage 34 immediately stops operating.
The type of the Y-axis motor 31 or the X-axis motor 32 can be selected according to actual needs, for example, in an application scenario, the Y-axis motor 31 is a 28-lead screw stepping motor, and the X-axis motor 32 is a 20-lead screw stepping motor.
In order to fix and protect the X-axis motor 32, in the present embodiment, the motor coupling 35 is provided with the motor receiving groove 353, and the X-axis motor 32 is fixed in the motor receiving groove 353.
Referring to fig. 16, the present invention also provides an intelligent microscope system, which includes a client 100, a server 200, a user terminal 300 and a microscope 400 according to the foregoing embodiment, wherein the lower computer is wirelessly or wiredly connected to the client 100, the client 100 is remotely connected to the server 200, the server 200 is remotely connected to the client 300, and the lower computer is configured to control an electric loading mechanism to move a slide to a window 11, control a Z-axis motor 44 to rotate to complete focusing, and control a camera 41 to scan to obtain a micrograph, and upload the micrograph to the server 200;
the server 200 is configured to perform artificial intelligence recognition on the photomicrograph to obtain an artificial intelligence analysis result, push the artificial intelligence analysis result to an expert system for artificial authentication to obtain an artificial authentication analysis result, generate a conclusion report by combining the artificial intelligence analysis result and the artificial authentication analysis result, and send the conclusion report to the user terminal 300.
As shown in fig. 17, in this embodiment, the specific steps of the lower computer controlling the Z-axis motor to rotate to complete focusing include:
s1: and controlling a Z-axis motor to drive the lens barrel to reach a focusing initial position, and taking a preset initial step length as a current step length.
Wherein the focus start position is a position in a lens barrel stroke.
S2: and controlling a Z-axis motor to drive the lens barrel to move downwards in a stepping manner at the current step length, controlling the camera to capture images and calculating the image definition after each movement until the lens barrel reaches an ultimate focusing position, wherein the ultimate focusing position is below a focusing starting position.
In this embodiment, the motor is a stepping motor, and the stepping motor rotates in a stepping manner to drive the lens barrel to move in a stepping manner. The limit focusing position is the lowest position of the lens barrel stroke. After the lens barrel moves downwards step by current step length each time, the camera captures an image and calculates the definition of the image, and in the embodiment, the definition of the image is calculated by an edge detection Laplacian operator or a Roberts gradient function.
S3: and selecting the position of the lens barrel when the image definition is maximum as a reference position.
Wherein the lens barrel moves overIn the process, the number of steps and the image definition of each downward stepping movement of the lens barrel are recorded (I)i，Zi) I denotes the number of times the lens barrel is moved in steps, IiRepresenting the image clarity, Z, of the lens barrel after the ith step movementiRepresenting the total step size after the ith step movement of the lens barrel. By comparing all the records, the position of the lens barrel at which the image definition is the maximum can be found.
Steps S1 to S3 are to select the position of the lens barrel at the time of the maximum image sharpness between the focus start position and the limit focus position as a reference position, and to complete the first round of focusing (coarse adjustment).
S4: and judging whether the current step length is smaller than a preset threshold value, if so, performing step S5, and if not, performing step S6.
In this embodiment, a ratio of the preset initial step length to the preset threshold is an integral multiple of 2, for example, 32. .
S5: and controlling a Z-axis motor to drive the lens barrel to reach the reference position, and finishing focusing.
If the current step size is smaller than the preset threshold, it indicates that the stepping distance of the lens barrel has reached the minimum, and the stepping distance cannot be reduced any more, so that focusing needs to be finished.
S6: and controlling the Z-axis motor to drive the lens barrel to reach a position above the reference position and away from the reference position by the current step length.
After the reference position is determined, the lens barrel can retract to a position above the reference position and away from the reference position by the current step length.
S7: the current step size is replaced by half the current step size.
Here, after step S7, the current step size becomes smaller.
S8: controlling a Z-axis motor to drive a lens barrel to move downwards in a stepping manner at the current step length;
s9: controlling a camera to capture an image and calculating the image definition after the lens barrel moves;
s10: and judging whether the image definition is greater than that of the last position of the lens barrel, if not, taking the last position of the lens barrel as a reference position, and performing the step S4 again, and if so, performing the step S8 again.
Here, steps S4 to S10 are to find the maximum image sharpness near the reference position, and to complete the second wheel focusing (fine adjustment). By two-wheel focusing, the maximum image definition can be found, i.e. the lens barrel is in the best position.
In this embodiment, the image sharpness is calculated by an edge detection Laplacian operator or a Roberts gradient function.
The focusing process of the automatic focusing method of the present embodiment will be described below by a specific example. In this specific example, the preset initial step size is 0.64mm, the preset numerical value is 2, the preset threshold value is 0.02mm, the coordinate of the uppermost position of the lens barrel stroke is assumed to be 0mm, the slide replacement position is 10mm, the focusing start position is 50mm, the limit focusing position is 60mm, and the lens barrel position corresponding to the actual maximum image sharpness is assumed to be 55.00 mm.
Proceeding to step S1, the lens barrel reaches the focusing start position;
proceeding to step S2, the lens barrel reaches the extreme focusing position;
in step S3, the position of the lens barrel at which the image clarity is the maximum is 55.12mm, that is, the reference position is 55.12 mm.
Step S4 is carried out, and the current step length of 0.64mm is larger than 0.02 mm;
proceeding to step S6, the barrel reaches a position above the reference position by 0.64mm from the reference position, that is, 55.12mm-0.64mm is 54.48 mm;
step S7 is performed, and the current step size becomes 0.32 mm;
proceeding to step S8, the barrel is moved down to reach position 54.80 mm;
step S9 is carried out, and the image definition at 54.80mm is calculated;
step S10 is carried out, the image definition at 54.80mm is larger than that at 54.48mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.12 mm;
step S9 is carried out, and the image definition at the position of 55.12mm is calculated;
step S10 is carried out, the image definition at 55.12mm is larger than that at 54.80mm, and step S8 is carried out again;
proceeding to step S8, the lens barrel moves down to reach position 55.44 mm;
step S9 is carried out, and the image definition at the position of 55.44mm is calculated;
performing step S10, the image clarity at 55.44mm is not more than the image clarity at 55.12mm, taking 55.12mm as the reference position, and performing step S4 again;
step S4 is carried out, and the current step length of 0.32mm is larger than 0.02 mm;
proceeding to step S6, the lens barrel reaches a position above the reference position by 0.32mm from the reference position, that is, 55.12mm-0.32mm is 54.80 mm;
step S7 is performed, and the current step size becomes 0.16 mm;
proceeding to step S8, the barrel is moved down to reach position 54.96 mm;
step S9 is carried out, and the image definition at 54.96mm is calculated;
step S10 is carried out, the image definition at 54.96mm is larger than that at 54.80mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.12 mm;
step S9 is carried out, and the image definition at the position of 55.12mm is calculated;
performing step S10, the image clarity at 55.12mm is not more than the image clarity at 54.96mm, 54.96mm is taken as a reference position, and performing step S4 again;
step S4 is carried out, and the current step length of 0.16mm is larger than 0.02 mm;
proceeding to step S6, the lens barrel reaches a position above the reference position by 0.16mm from the reference position, that is, 54.96mm-0.16 mm-54.80 mm;
step S7 is performed, and the current step size becomes 0.08 mm;
proceeding to step S8, the lens barrel moves down to reach position 54.88 mm;
step S9 is carried out, and the image definition at the position of 54.88mm is calculated;
step S10 is carried out, the image definition at 54.88mm is larger than that at 54.80mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 54.96 mm;
step S9 is carried out, and the image definition at 54.96mm is calculated;
step S10 is carried out, the image definition at 54.96mm is larger than that at 54.88mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.04 mm;
step S9 is carried out, and the image definition at 55.04mm is calculated;
performing step S10, the image clarity at 55.04mm is not more than the image clarity at 54.96mm, 54.96mm is taken as a reference position, and performing step S4 again;
step S4 is carried out, and the current step length of 0.08mm is larger than 0.02 mm;
proceeding to step S6, the lens barrel reaches a position above the reference position by 0.08mm from the reference position, that is, 54.96mm-0.08 mm-54.88 mm;
step S7 is performed, and the current step size becomes 0.04 mm;
proceeding to step S8, the barrel is moved down to reach position 54.92 mm;
step S9 is carried out, and the image definition at 54.92mm is calculated;
step S10 is carried out, the image definition at 54.92mm is larger than that at 54.88mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 54.96 mm;
step S9 is carried out, and the image definition at 54.96mm is calculated;
step S10 is carried out, the image definition at 54.96mm is larger than that at 54.92mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.00 mm;
step S9 is carried out, and the image definition at the position of 55.00mm is calculated;
step S10 is carried out, the image definition at 55.00mm is larger than that at 54.96mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.04 mm;
step S9 is carried out, and the image definition at 55.04mm is calculated;
performing step S10, with the image clarity at 55.04mm not greater than the image clarity at 55.00mm, taking 55.00mm as the reference position, and performing step S4 again;
step S4 is carried out, and the current step length of 0.04mm is larger than 0.02 mm;
proceeding to step S6, the barrel reaches a position above the reference position by 0.04mm from the reference position, that is, 55.00mm-0.04mm ═ 54.96 mm;
step S7 is performed, and the current step size becomes 0.02 mm;
proceeding to step S8, the barrel is moved down to reach position 54.98 mm;
step S9 is carried out, and the image definition at 54.98mm is calculated;
step S10 is carried out, the image definition at 54.98mm is larger than that at 54.96mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.00 mm;
step S9 is carried out, and the image definition at the position of 55.00mm is calculated;
step S10 is carried out, the image definition at 55.00mm is larger than that at 54.98mm, and step S8 is carried out again;
proceeding to step S8, the barrel is moved down to reach position 55.02 mm;
step S9 is carried out, and the image definition at the position of 55.02mm is calculated;
performing step S10, with the image clarity at 55.02mm being not greater than that at 55.00mm, taking 55.00mm as a reference position, and performing step S4 again;
step S4 is carried out, and the current step size of 0.02mm is equal to (not less than) 0.02 mm;
step S7 is performed, and the current step size becomes 0.01 mm;
proceeding to step S8, the barrel is moved down to reach position 55.01 mm;
performing step S9, calculating image sharpness at 55.01;
performing step S10, with the image clarity at 55.01mm not greater than the image clarity at 55.00mm, taking 55.00mm as the reference position, and performing step S4 again;
step S4 is carried out, and the current step length of 0.01mm is smaller than 0.02 mm;
step S5 is performed to end focusing, and the lens barrel is located exactly at 55 mm.
Through the mode, the microscope and the intelligent microscope system of the embodiment of the invention carry out position adjustment in the Y-axis direction and the X-axis direction on the objective table through the Y-axis motor and the X-axis motor, and carry out adjustment in the Z-axis direction on the lens barrel through the Z-axis motor, so that the visual field can be automatically adjusted and the automatic focusing can be automatically realized, the response speed of the motor is high, the control precision is high, and the adjustment speed and the adjustment precision can be improved.
The above description is only an example of the present application and is not intended to limit the scope of the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Claims (10)
1. The microscope is characterized by comprising a bottom shell, an upper shell, an electric object carrying mechanism, a light path mechanism and a lower computer, wherein the upper shell is positioned on the bottom shell, the upper shell and the bottom shell are of a hollow structure and are communicated with each other, a window is formed in the upper surface of the bottom shell, the electric object carrying mechanism is positioned in the bottom shell and can carry a glass slide and move in an X-axis plane and a Y-axis plane, the light path mechanism comprises a camera, a lens barrel, an objective lens, a Z-axis motor and a light source, the lens barrel is arranged along the Z axis and is positioned above the window, the light source is positioned at the bottom of the bottom shell, the camera is positioned at the top end of the lens barrel, the objective lens is positioned at the bottom end of the lens barrel, the camera, the lens barrel, the objective lens, the glass slide and the light source form a light path, the Z-axis motor is positioned in the upper shell, And controlling the Z-axis motor to rotate and controlling the camera to shoot the photomicrograph.
2. The microscope of claim 1, wherein the motorized stage mechanism comprises a Y-axis motor, an X-axis motor, a sliding track, a stage, a motor coupler, a sliding coupler, a first sliding rod, a second sliding rod, and a lead screw, wherein the sliding track is slidably connected with a sliding block, the Y-axis motor is fixed on the sliding track to drive the sliding block to slide along a first direction, the motor coupler is fixed on the sliding block, the first sliding rod is fixed relative to the sliding track and is arranged along the first direction, the sliding coupler is slidably connected on the first sliding rod, two ends of the second sliding rod are respectively fixed with the motor coupler and the sliding coupler, the lead screw and the second sliding rod are parallel to each other and are arranged along a second direction perpendicular to the first direction, the X-axis motor is fixed on the motor coupler and is coupled with one end of the lead screw to drive the lead screw to rotate, the other end and the sliding connection piece of lead screw rotate to be connected, second slide bar and lead screw all pass the objective table, second slide bar and objective table sliding connection, lead screw and objective table threaded connection, the objective table is used for carrying on the slide, the next machine is used for controlling Y axle motor and X axle motor and rotates.
3. The microscope of claim 2, wherein the motorized stage further comprises two fixing seats, and the two fixing seats are respectively located at two ends of the first sliding rod and are fixedly connected with two ends of the first sliding rod.
4. The microscope of claim 3, wherein the fixing base has three screw holes along the Z-axis, and each fixing base has three screws, wherein two screws are threaded through the two screw holes of the fixing base after passing through the bottom of the bottom shell, and the other screw is threaded through the bottom of the bottom shell after passing through the other screw hole of the fixing base.
5. The microscope of claim 2, wherein the bottom end of the motor coupling is provided with a mounting bracket, the mounting bracket is positioned between the slide block and the guide rail sliding table and is close to the end part of the guide rail sliding table, and the mounting bracket is provided with a first limit switch.
6. The microscope of claim 5, wherein the side of the motor coupling facing the stage is provided with a mounting hole, and a second limit switch is provided in the mounting hole.
7. The microscope of claim 2, wherein the motor coupling has a motor receiving slot therein, and the second motor is secured within the motor receiving slot.
8. An intelligent microscope system is characterized by comprising a client, a server, a user terminal and a microscope according to any one of claims 1 to 7, wherein the lower computer is in wireless connection or wired connection with the client, the client is in remote connection with the server, the server is in remote connection with the client, and the lower computer is used for controlling an electric loading mechanism to move a slide to a window, controlling a Z-axis motor to rotate to complete focusing, controlling a camera to scan to obtain a micrograph and uploading the micrograph to the server;
the server is used for carrying out artificial intelligence recognition on the photomicrographs to obtain artificial intelligence analysis results, pushing the artificial intelligence analysis results to the expert system for artificial authentication to obtain artificial authentication analysis results, generating a conclusion report by combining the artificial intelligence analysis results and the artificial authentication analysis results, and sending the conclusion report to the user terminal.
9. The intelligent microscope system of claim 8, wherein the specific step of controlling the Z-axis motor to rotate by the lower computer to complete focusing comprises:
s1: controlling a Z-axis motor to drive a lens barrel to reach a focusing initial position, and taking a preset initial step length as a current step length;
s2: controlling a Z-axis motor to drive a lens barrel to move downwards in a stepping mode according to the current step length, controlling a camera to capture images and calculating the image definition after each movement until the lens barrel reaches an ultimate focusing position, wherein the ultimate focusing position is below a focusing starting position;
s3: selecting the position of the lens barrel when the image definition is maximum as a reference position;
s4: judging whether the current step length is smaller than a preset threshold value, if so, performing step S5, and if not, performing step S6;
s5: controlling a Z-axis motor to drive a lens barrel to reach a reference position, and finishing focusing;
s6: controlling a Z-axis motor to drive a lens barrel to reach a position above the reference position and away from the reference position by the current step length;
s7: replacing the current step length by half of the current step length;
s8: controlling a Z-axis motor to drive a lens barrel to move downwards in a stepping manner at the current step length;
s9: controlling a camera to capture an image and calculating the image definition after the lens barrel moves;
s10: and judging whether the image definition is greater than that of the last position of the lens barrel, if not, taking the last position of the lens barrel as a reference position, and performing the step S4 again, and if so, performing the step S8 again.
10. The intelligent microscope system of claim 9, wherein the image sharpness is calculated by edge detection Laplacian operator or Roberts gradient function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110382334.XA CN113064269A (en) | 2021-04-09 | 2021-04-09 | Microscope and intelligent microscope system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110382334.XA CN113064269A (en) | 2021-04-09 | 2021-04-09 | Microscope and intelligent microscope system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113064269A true CN113064269A (en) | 2021-07-02 |
Family
ID=76566327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110382334.XA Pending CN113064269A (en) | 2021-04-09 | 2021-04-09 | Microscope and intelligent microscope system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113064269A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101706609A (en) * | 2009-11-23 | 2010-05-12 | 常州达奇信息科技有限公司 | Image processing based fast automatic focusing method of microscope |
| CN202610320U (en) * | 2012-05-08 | 2012-12-19 | 东莞市天域半导体科技有限公司 | Height fine-adjustment device for high-temperature CVD (chemical vapor deposition) growth chamber |
| CN103399398A (en) * | 2013-07-30 | 2013-11-20 | 济南华天恒达科技有限公司 | Automatically-focused microscope |
| CN207216138U (en) * | 2017-09-19 | 2018-04-10 | 湖南科技大学 | A kind of micro- automatic control device of monotubular |
| CN109031638A (en) * | 2018-08-23 | 2018-12-18 | 深圳市瑞图生物技术有限公司 | The fast automatic focusing method of biomicroscope |
| CN209327673U (en) * | 2019-03-06 | 2019-08-30 | 数优(苏州)人工智能科技有限公司 | Artificial intelligence microscope |
| CN209496196U (en) * | 2019-01-29 | 2019-10-15 | 许之敏 | A kind of continuous vari-focus microscopic structure |
| CN110687671A (en) * | 2019-09-29 | 2020-01-14 | 刘杰 | Microscope and control method thereof |
| CN111679419A (en) * | 2020-05-25 | 2020-09-18 | 苏州米特希赛尔人工智能有限公司 | Microscope multi-slide objective table movement control system |
| US20210082570A1 (en) * | 2019-09-13 | 2021-03-18 | Celly.AI | Artificial intelligence (ai) powered analysis of objects observable through a microscope |
-
2021
- 2021-04-09 CN CN202110382334.XA patent/CN113064269A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101706609A (en) * | 2009-11-23 | 2010-05-12 | 常州达奇信息科技有限公司 | Image processing based fast automatic focusing method of microscope |
| CN202610320U (en) * | 2012-05-08 | 2012-12-19 | 东莞市天域半导体科技有限公司 | Height fine-adjustment device for high-temperature CVD (chemical vapor deposition) growth chamber |
| CN103399398A (en) * | 2013-07-30 | 2013-11-20 | 济南华天恒达科技有限公司 | Automatically-focused microscope |
| CN207216138U (en) * | 2017-09-19 | 2018-04-10 | 湖南科技大学 | A kind of micro- automatic control device of monotubular |
| CN109031638A (en) * | 2018-08-23 | 2018-12-18 | 深圳市瑞图生物技术有限公司 | The fast automatic focusing method of biomicroscope |
| CN209496196U (en) * | 2019-01-29 | 2019-10-15 | 许之敏 | A kind of continuous vari-focus microscopic structure |
| CN209327673U (en) * | 2019-03-06 | 2019-08-30 | 数优(苏州)人工智能科技有限公司 | Artificial intelligence microscope |
| US20210082570A1 (en) * | 2019-09-13 | 2021-03-18 | Celly.AI | Artificial intelligence (ai) powered analysis of objects observable through a microscope |
| CN110687671A (en) * | 2019-09-29 | 2020-01-14 | 刘杰 | Microscope and control method thereof |
| CN111679419A (en) * | 2020-05-25 | 2020-09-18 | 苏州米特希赛尔人工智能有限公司 | Microscope multi-slide objective table movement control system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107529011B (en) | A kind of motorized zoom lens control method | |
| US8111938B2 (en) | System and method for fast approximate focus | |
| CN103399398B (en) | Automatically-focused microscope | |
| CN108732094B (en) | Three-dimensional image information acquisition method and device for cell morphology reconstruction | |
| CN108303792A (en) | It is a kind of can the vertical microscopical method of adjustment of two level adjustment | |
| CN103499870A (en) | Automatic focusing equipment of high-pixel module | |
| CN109781745A (en) | A kind of optical element surface defects detection Atomatic focusing method and device | |
| CN102253483A (en) | Automatic upright metallurgical microscope | |
| CN210534819U (en) | Miniature microscopic image acquisition device based on mobile phone | |
| CN101408660B (en) | Automatic multiple-focus focusing device and method thereof | |
| CN113064269A (en) | Microscope and intelligent microscope system | |
| CN106254742B (en) | Double-camera image measuring mechanism | |
| CN202177736U (en) | Automatic straight-arranged metallographic microscope | |
| CN113406764B (en) | Optical lens aligning method and system | |
| CN117170081A (en) | Automatic focusing method based on image definition identification | |
| CN113064267A (en) | Automatic focusing method of microscope | |
| CN216669776U (en) | Strain gauge defect recognition equipment based on machine vision | |
| CN107009023B (en) | A kind of focus control and its focus adjustment method adjusting laser focal | |
| CN110049300A (en) | Projector lens focus method based on variable step motion control | |
| CN113899748A (en) | Strain gauge defect identification equipment and method based on machine vision | |
| CN111679420B (en) | Automatic digital pathological section scanning system and method | |
| CN210927783U (en) | Automatic focusing device convenient to operate | |
| CN210514761U (en) | Multi-focus visual lens with variable focal length | |
| CN214392796U (en) | Light path fine adjustment device for 3D printing equipment | |
| CN111999296A (en) | Big data based self-learning workpiece intelligent appearance detection system and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210702 |
|
| RJ01 | Rejection of invention patent application after publication |