CN113063785A - Focusing adjustment system for analyzing urine visible components and focusing method thereof - Google Patents
Focusing adjustment system for analyzing urine visible components and focusing method thereof Download PDFInfo
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- CN113063785A CN113063785A CN202110306096.4A CN202110306096A CN113063785A CN 113063785 A CN113063785 A CN 113063785A CN 202110306096 A CN202110306096 A CN 202110306096A CN 113063785 A CN113063785 A CN 113063785A
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 210000002700 urine Anatomy 0.000 title claims abstract description 27
- 230000033001 locomotion Effects 0.000 claims abstract description 25
- 239000002245 particle Substances 0.000 claims abstract description 19
- 238000003384 imaging method Methods 0.000 claims abstract description 17
- 239000013049 sediment Substances 0.000 claims abstract description 17
- 230000002485 urinary effect Effects 0.000 claims abstract description 6
- 238000004458 analytical method Methods 0.000 claims description 12
- 230000007246 mechanism Effects 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 abstract description 11
- 239000007788 liquid Substances 0.000 description 12
- 238000005286 illumination Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000005670 electromagnetic radiation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 229910052724 xenon Inorganic materials 0.000 description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 2
- 206010065042 Immune reconstitution inflammatory syndrome Diseases 0.000 description 1
- 238000013528 artificial neural network Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/493—Physical analysis of biological material of liquid biological material urine
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- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a focusing adjustment system for analyzing urine visible components and a focusing method thereof, wherein the focusing adjustment system comprises the following steps: s1: placing a sample container in a microscope system, dividing an imaging target surface of the sample container into a plurality of using areas at least comprising a sample shooting area and a focusing area, and setting a mark in the focusing area; s2: connecting the sample container with a cam, and driving the cam to rotate through a motor; s3: starting a motor to enable the sample container to do reciprocating motion, and ensuring that the sample appears on a front focal plane of the microscope system in a single motion period; s4: matching the discharge frequency of the strobe lamp with the reciprocating motion period of the sample container so as to obtain a high-resolution urinary sediment particle image; the invention greatly reduces the stroke of searching the focus and shortens the searching time, so the design does not need to consider the anti-backlash design of the lead screw in the linear motion process of the motor, and the requirement on a motor motion system is reduced.
Description
Technical Field
The invention relates to the field of medical equipment, in particular to a focusing adjustment system for analyzing urine visible components and a focusing method thereof.
Background
In the year 2000, the flow urine visible component analyzer product of IRIS corporation, usa, was put on the market formally by sucking a urine sample into a flow cell, using a sheath flow technique, i.e. aligning a capillary tube to a small-bore tube, and ejecting a cell suspension from the capillary tube. Meanwhile, the cell suspension flows through the sensitive area together with the sheath liquid flowing out of the periphery, so that the cell suspension is ensured to form a single arranged cell flow in the middle, the periphery is surrounded by the sheath liquid, the tangible component independently passes through the photographing area, the central deviation range of the tangible component sample can be controlled within 5 mu m, the imaging effect is good, and the repeatability is excellent; the sedimentation type urine formed components naturally settle in the sedimentation tank, and the deviation range of the formed component sample center is about 40 mu m.
However, in the existing photographing process, a long time is often needed to find a focus, so that the photographing process is relatively long and occupies a large part of time of component analysis.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a focusing adjustment system for analyzing urine visible components and a focusing method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that: a focusing adjustment system for analyzing urine visible components and a focusing method thereof comprise the following steps: s1: placing a sample container in a microscope system, dividing an imaging target surface of the sample container into a plurality of using areas at least comprising a sample shooting area and a focusing area, and setting a mark in the focusing area; s2: connecting the sample container with a cam, and driving the cam to rotate through a motor; s3: starting a motor to enable the sample container to do reciprocating motion, and ensuring that the sample appears on a front focal plane of the microscope system in a single motion period; s4: the discharge frequency of the strobe lamp is matched with the reciprocating motion period of the sample container, so that a high-resolution urinary sediment particle image is obtained.
In a preferred embodiment of the present invention, the photographing region and the focusing region are independent of each other.
In a preferred embodiment of the present invention, the motor drives the cam to move in a single direction.
In a preferred embodiment of the invention, the strobe light is an LED light source.
In a preferred embodiment of the invention, the discharge frequency of the strobe lamp is 30-40 times/second.
In a preferred embodiment of the present invention, in S4, the urinary sediment particle image is magnified and then photographed by a high resolution camera.
In a preferred embodiment of the invention, only one drop of urine sample is used in the sample container.
In a preferred embodiment of the present invention, a sheath fluid is injected into the sample container.
In a preferred embodiment of the present invention, the sheath fluid flows stably in the sample container.
The invention also provides a focusing regulation system for analyzing urine visible components, which is characterized in that: the focusing mechanism is internally provided with a motor and a cam, the motor drives the cam, and the sample container reciprocates under the action of the cam; still include the strobe light, the strobe light with motor electric connection.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) the invention sets the mark on the sample container, and makes the mark image on the area of the imaging target surface for focusing, thus, no focusing liquid is needed in the focusing process, the consumption of reagent consumables is reduced, and simultaneously, the uniformity of the background of the shot image is ensured without mutual influence of the two imaging areas.
(2) The focusing movement mechanism adopts a form that the motor is matched with the cam, the motor drives the cam to move along one direction, the sample container does reciprocating motion under the action of the cam, the position of a sample to be shot in one movement period is bound to appear on the front focal plane of the microscope, the stroke of searching a focus is greatly reduced, the searching time is shortened, the anti-backlash design of a lead screw in the linear movement process of the motor is not considered in the design, the requirement on a motor movement system is reduced, and the effect of saving cost is achieved.
(3) The LED is used as a light source, and a xenon lamp is currently generally used, needs a special power supply module, and has high voltage, large electromagnetic radiation and high price. The LED has high reliability, long service life and low price. The problems that the brightness of an LED serving as a light source is insufficient in high-speed photomicrography and the noise is large after the LED is imaged by a photoelectric coupling device are solved through excellent optical design and electric appliance design, the light source provides illumination with uniform image surface gray scale and sufficient brightness for a shooting sample after passing through an illumination system, the mutual matching of the illumination system and the photomicrography system is completely different from that of a traditional microscope, and the optimal matching state of illumination and imaging is determined on the basis of a large number of experiments.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts;
fig. 1 is a flow chart of a preferred embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
If the specification states a component, feature, structure, or characteristic "may", "might", or "could" be included, that particular component, feature, structure, or characteristic is not required to be included
In the description of the present invention, the terms "center" and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the scope of the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
As shown in figure 1, a focusing method of a focusing adjustment system for urine visible component analysis is characterized in that an imaging target surface is innovatively divided into a plurality of using areas in the focusing process, wherein the using areas at least comprise an area for shooting a sample and an area for focusing, a mark is arranged on a sample container and is imaged in the area for focusing of the imaging target surface, so that no focusing liquid is needed in the focusing process, the consumption of reagent consumables is reduced, and meanwhile, the two imaging areas do not influence each other and the uniformity of the background of a shot image is ensured; the focusing mechanism of the microscope is different from all focusing mechanisms on the market at present, the focusing mechanism of the company adopts a mode that a motor is matched with a cam, the motor drives the cam to move along one direction, a sample container does reciprocating motion under the action of the cam, the position of a sample to be shot in one motion period is necessarily on the front focal plane of the microscope, the stroke of searching a focus is greatly reduced, the searching time is shortened, the design does not need to consider the anti-backlash design of a lead screw (the clearance of the lead screw thread matched with a nut) in the linear motion process of the motor, and the requirement on a motor motion system is reduced.
The invention also provides a focusing regulation system for analyzing urine visible components, which is characterized in that: the focusing mechanism is internally provided with a motor and a cam, the motor drives the cam, and the sample container reciprocates under the action of the cam; still include the strobe light, the strobe light with motor electric connection.
The invention sets the mark on the sample container, and makes the mark image on the area of the imaging target surface for focusing, thus, no focusing liquid is needed in the focusing process, the consumption of reagent consumables is reduced, and simultaneously, the uniformity of the background of the shot image is ensured without mutual influence of the two imaging areas.
The focusing movement mechanism adopts a form that the motor is matched with the cam, the motor drives the cam to move along one direction, the sample container does reciprocating motion under the action of the cam, the position of a sample to be shot in one movement period is bound to appear on the front focal plane of the microscope, the stroke of searching a focus is greatly reduced, the searching time is shortened, the anti-backlash design of a lead screw in the linear movement process of the motor is not considered in the design, the requirement on a motor movement system is reduced, and the effect of saving cost is achieved.
The LED is used as a light source, and a xenon lamp is currently generally used, needs a special power supply module, and has high voltage, large electromagnetic radiation and high price. The LED has high reliability, long service life and low price. The problems that the brightness of an LED serving as a light source is insufficient in high-speed photomicrography and the noise is large after the LED is imaged by a photoelectric coupling device are solved through excellent optical design and electric appliance design, the light source provides illumination with uniform image surface gray scale and sufficient brightness for a shooting sample after passing through an illumination system, the mutual matching of the illumination system and the photomicrography system is completely different from that of a traditional microscope, and the optimal matching state of illumination and imaging is determined on the basis of a large number of experiments.
The visible component analyzer to which the present invention is applied is: the 27 visible components in urine are effectively and automatically classified and counted by adopting a planar flow cytometry technology and a high-speed photography technology and using a neural network image recognition technology.
The aspirated sample is pushed into the particle imaging chamber by the sample addition syringe, and the urine sediment flows in the center of the particle imaging chamber by the sheath fluid. The cavity structure of the particle imaging chamber is designed into a symmetrical structure, and the sample liquid is positioned in the center of the channel after being injected into the particle imaging chamber. Therefore, the liquid flow velocity at the two sides of the sample liquid layer can be kept consistent, the posture in the particle flow is kept stable, and the particle rolling phenomenon is inhibited. In addition, the sheath liquid on both sides of the sample liquid can also be subjected to precise flow control, and the position of the laminar flow formed by the sample liquid is actively controlled, so that the particle posture is adjusted.
In the flowing process of the sample liquid, the flash lamp irradiates the urine visible components once at certain intervals, and the continuous and static urinary sediment particle optical image (stroboscopic effect) can be obtained by the very high discharge speed (about 1 microsecond) of the flash lamp in cooperation with the proper flow rate of the sample. After the image is amplified, a CCD camera with high resolution is used for shooting to obtain electronic image information of various shape components.
The images are displayed on a screen after being calculated and processed by an image processor, and are classified and counted according to the sizes of the particles, the processed images are identified by a computer, segmentation and feature extraction are carried out according to the widths and the lengths of the particles in the images, the images are classified into different grades and counted, and the counting values of the particles under the different grades are obtained.
Classifying by automatic particle analysis software to obtain the proportion of each component in a specific grade, and multiplying the proportion by the total number of particles of the grade to calculate the particle number of each component.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. A focusing method of a focus adjustment system for urine sediment analysis, comprising the steps of:
s1: placing a sample container in a microscope system, dividing an imaging target surface of the sample container into a plurality of using areas at least comprising a sample shooting area and a focusing area, and setting a mark in the focusing area;
s2: connecting the sample container with a cam, and driving the cam to rotate through a motor;
s3: starting a motor to enable the sample container to do reciprocating motion, and ensuring that the sample appears on a front focal plane of the microscope system in a single motion period;
s4: the discharge frequency of the strobe lamp is matched with the reciprocating motion period of the sample container, so that a high-resolution urinary sediment particle image is obtained.
2. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: the photographing region and the focusing region are independent of each other.
3. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: the motor drives the cam to move along a single direction.
4. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: the stroboscopic lamp adopts an LED light source.
5. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: the discharge frequency of the stroboscopic lamp is 30-40 times/second.
6. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: in S4, the urinary sediment particle image is magnified and photographed by a high-resolution camera.
7. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: only one drop of urine sample is used in the sample container.
8. The focusing method of a focusing adjustment system for urine sediment analysis according to claim 1, wherein: sheath fluid is injected into the sample container.
9. The focusing method of a focusing adjustment system for urine sediment analysis as set forth in claim 8, wherein: the sheath fluid flows stably within the sample container.
10. A focus adjustment system for analysis of urine sediment according to claim 1 wherein: the focusing mechanism is internally provided with a motor and a cam, the motor drives the cam, and the sample container reciprocates under the action of the cam; still include the strobe light, the strobe light with motor electric connection.
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| CN202110306096.4A CN113063785A (en) | 2021-03-23 | 2021-03-23 | Focusing adjustment system for analyzing urine visible components and focusing method thereof |
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| CN202110306096.4A CN113063785A (en) | 2021-03-23 | 2021-03-23 | Focusing adjustment system for analyzing urine visible components and focusing method thereof |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1243963A (en) * | 1998-07-17 | 2000-02-09 | 富士摄影胶片株式会社 | Camera with lens driven by motor |
| CN101702053A (en) * | 2009-11-13 | 2010-05-05 | 长春迪瑞实业有限公司 | Method for automatically focusing microscope system in urinary sediment examination equipment |
| CN102062929A (en) * | 2010-11-27 | 2011-05-18 | 长春迪瑞医疗科技股份有限公司 | Automatic focusing method and device for microscope system |
| CN104238235A (en) * | 2014-07-30 | 2014-12-24 | 中国科学院长春光学精密机械与物理研究所 | One-way-drive reciprocating motion type focusing mechanism |
| CN107091800A (en) * | 2017-06-06 | 2017-08-25 | 深圳小孚医疗科技有限公司 | Focusing system and focus method for micro-imaging particle analysis |
| CN107144520A (en) * | 2017-06-06 | 2017-09-08 | 深圳小孚医疗科技有限公司 | Particle imaging chamber and focusing system for micro-imaging particle analysis |
| CN210181293U (en) * | 2019-08-02 | 2020-03-24 | 深圳市瀚德标检生物工程有限公司 | Microscope device capable of automatically focusing |
-
2021
- 2021-03-23 CN CN202110306096.4A patent/CN113063785A/en active Pending
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|---|---|---|---|---|
| CN1243963A (en) * | 1998-07-17 | 2000-02-09 | 富士摄影胶片株式会社 | Camera with lens driven by motor |
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| CN107144520A (en) * | 2017-06-06 | 2017-09-08 | 深圳小孚医疗科技有限公司 | Particle imaging chamber and focusing system for micro-imaging particle analysis |
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Application publication date: 20210702 |