CN113138190A - Urine sample detection device and detection method - Google Patents

Urine sample detection device and detection method Download PDF

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Publication number
CN113138190A
CN113138190A CN202010053766.1A CN202010053766A CN113138190A CN 113138190 A CN113138190 A CN 113138190A CN 202010053766 A CN202010053766 A CN 202010053766A CN 113138190 A CN113138190 A CN 113138190A
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urine sample
counting cell
move
target position
counting
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沈志龙
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Suzhou Mairui Technology Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434Optical arrangements
    • G01N15/1436Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012Red blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016White blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486Counting the particles

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  • Physics & Mathematics (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Immunology (AREA)
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Abstract

The invention provides urine sample detection equipment and a detection method, wherein the urine sample detection equipment comprises a counting cell, an image acquisition device, a driving device and a control device, wherein the counting cell is used for bearing a urine sample to be detected; the image pickup unit of the image acquisition device is used for shooting the urine sample in the visual field area; the driving device is connected to the counting pool and used for driving the counting pool to move so as to focus the urine sample; the control device is coupled to the driving device and is used for detecting the movement direction of the counting cell and controlling the movement mode of the counting cell, when the counting cell is detected to move towards the target position along a second direction opposite to the preset first direction, the counting cell is moved to a position exceeding the target position by a preset distance along the second direction, and then the counting cell is driven to move to the target position along the first direction for focusing. The device and the method eliminate the return stroke difference of the focusing process during the detection of the urine sample, improve the definition of the shot picture and do not cause the increase of the cost.

Description

Urine sample detection device and detection method
Technical Field
The present invention relates to the field of urine sample testing, and more particularly, to a urine sample testing device and a testing method.
Background
When the urine sample is detected, when a microscope of a urine sample detection device (for example, a urine visible component analyzer) photographs the urine sample in the counting cell, the counting cell is driven to move in X, Y, Z three directions through a motor, an optical coupler and a related control program, so that the counting cell can move to a target position which is previously focused and recorded or set, and a clear picture can be taken.
However, the counting cell has a return difference (null return) in the moving process, which is expressed in that if the final direction of the counting cell moving to a certain position in a certain dimension (X, Y or Z direction) is different, the actual arrival position after the counting cell moving to the position is also different, and the deviation is the return difference. The return stroke difference is generated by different thread pitches, elastic structures, friction and gaps among mechanical parts, and influences the tracking and positioning precision in the horizontal direction and influences the definition of cell photos in the vertical direction. At present, the method for reducing the influence of return stroke difference on the horizontal direction adopts a gap eliminating nut, and no measure is provided in the vertical direction.
Although the backlash difference can be basically eliminated by adopting the backlash eliminating nut, the following defects exist:
1) the material cost and the assembly time are increased;
2) the anti-backlash nut can increase the transmission load, so that the movement speed of the counting cell is slowed down, and the testing speed is influenced;
3) mechanical abrasion is increased, so that the service life of the transmission structure is greatly reduced;
4) this structure cannot be applied in the vertical direction.
Therefore, there is a need in the art for a new urine sample testing device and method to solve the above problems.
Disclosure of Invention
The present invention has been made to solve the above problems. According to an aspect of the present invention, there is provided a urine sample testing device comprising a counting cell, an image acquisition device, a driving device and a control device, wherein: the counting cell is used for bearing a urine sample to be tested; the image acquisition device comprises a camera unit, wherein the camera unit is used for shooting the urine sample in a visual field area so as to acquire a plurality of images of the urine sample; the driving device is connected to the counting cell and used for driving the counting cell to move so as to focus the urine sample; the control device is coupled to the driving device and used for detecting the movement direction of the counting cell and controlling the movement mode of the counting cell, wherein when the counting cell is detected to move towards the target position along a second direction opposite to the preset first direction, the control device controls the driving device to actuate the counting cell to move to a position exceeding the target position by a preset distance along the second direction and then drive the counting cell to move to the target position along the first direction for focusing.
According to another aspect of the present invention, there is provided a urine sample testing device comprising: the setting module is used for presetting a first direction of movement of a counting cell of the urine sample detection equipment; and the control module is used for detecting the movement direction of the counting pool and controlling the movement mode of the counting pool, wherein when the counting pool is detected to move to a target position along a second direction opposite to the first direction, the counting pool is firstly controlled to move to a position exceeding the target position by a preset distance along the second direction, and then the counting pool is controlled to move to the target position along the first direction.
According to another aspect of the present invention, there is provided a urine sample detection method applied to a urine sample detection apparatus including an image acquisition device, the urine sample detection method including: driving the counting pool to move to focus the urine sample, and detecting the moving direction of the counting pool, wherein when the counting pool is detected to move to a target position along a second direction opposite to a preset first direction, the counting pool is firstly driven to move to a position exceeding the target position by a preset distance along the second direction, and then the counting pool is driven to move to the target position along the first direction to focus; and controlling a camera unit of the image acquisition device to photograph the focused urine sample in the counting cell so as to acquire a plurality of images of the urine sample.
According to yet another aspect of the invention, there is provided a computer-readable storage medium containing computer-executable instructions capable, when executed by a processor, of performing the method as described above.
According to the urine sample detection device and the urine sample detection method provided by the embodiment of the invention, the return stroke difference of the microscope in the focusing process during urine sample detection is eliminated, the definition of the shot picture is improved, and only the urine sample detection flow is optimized, so that the cost is not increased.
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The above and other objects, features and advantages of the present invention will become more apparent by describing in more detail embodiments of the present invention with reference to the attached drawings. The accompanying drawings are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings, like reference numbers generally represent like parts or steps.
FIG. 1 shows a schematic structural view of a urine sample testing device according to one embodiment of the present invention.
FIG. 2 illustrates a schematic diagram of an exemplary path for driving the motion of the counting cell to eliminate the return difference according to one embodiment of the invention.
FIG. 3 shows a schematic block diagram of a urine sample testing device according to one embodiment of the present invention.
FIG. 4 illustrates an exemplary flow chart of a urine sample testing method according to one embodiment of the present invention.
FIG. 5 shows a schematic top view of a counting cell in a urine sample detection device according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, exemplary embodiments according to the present invention will be described in detail below with reference to the accompanying drawings. It is to be understood that the described embodiments are merely a subset of embodiments of the invention and not all embodiments of the invention, with the understanding that the invention is not limited to the example embodiments described herein. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the invention described herein without inventive step, shall fall within the scope of protection of the invention.
As described above, due to the difference in the return path, the microscope of the urine sample testing device may be not focused accurately, and the taken picture may be unclear.
In order to eliminate the return path difference, the invention provides a urine sample detection device and a urine sample detection method, wherein the urine sample detection device can comprise a counting cell, an image acquisition device, a driving device and a control device, wherein: the counting cell is used for bearing a urine sample to be tested; the image acquisition device comprises a camera unit, wherein the camera unit is used for shooting the urine sample in a visual field area so as to acquire a plurality of images of the urine sample; the driving device is connected to the counting cell and used for driving the counting cell to move so as to focus the urine sample; the control device is coupled to the driving device and is used for detecting the moving direction of the counting cell and controlling the moving mode of the counting cell, wherein: when the counting cell is detected to move to the target position along a second direction opposite to the preset first direction, the control device controls the driving device to drive the counting cell to move to a position exceeding the target position by a preset distance along the second direction and then drive the counting cell to move to the target position along the first direction for focusing.
The urine sample detection equipment and the urine sample detection method eliminate the return difference of the microscope focusing process during urine sample detection, improve the definition of the shot pictures, optimize the urine sample detection process only and avoid the increase of cost.
The urine sample test device and the urine sample test method according to the present invention will be described in detail below with reference to specific embodiments.
Example one
The present embodiments provide a urine sample testing device. As shown in fig. 1, fig. 1 shows a schematic structural view of a urine sample testing device 100 according to an embodiment of the present invention.
Referring to fig. 1, a urine sample test device 100 according to the present embodiment may include a counting chamber 10, an image capture device 20, a driving device 30, and a control device 40. It should be understood that the urine sample testing device 100 may also include well-known components, such as a transfer track, a sample injection mechanism, a sample injector, etc., and the present invention is not limited thereto. The urine sample testing device 100 may be any urine sample testing device known in the art, such as a urine visible component analyzer, a urinary sediment analyzer, and the like, but the invention is not limited thereto. The following description will be made by taking a urine visible component analyzer as an example.
The counting chamber 10 is used for carrying a urine sample to be tested, and the urine sample may contain various tangible components, such as red blood cells, white blood cells, leukocyte aggregates, bacteria, epithelial cells, crystals, casts, mucus threads, and sperms, and may further contain various impurities. Furthermore, the counting cell 10 is also used to provide a field of view for the photographing.
The image acquisition device 20 is used for magnifying, focusing and photographing the urine sample in the photographing field of view to obtain a plurality of images. Specifically, the image acquisition device 20 may include a microscope 22 and a camera unit 24.
The microscope 22 may include a stage 222 for carrying the counting cell 10, and an objective lens 224 for magnifying the object to be measured in the photographing field of view. In use, the stage 222 and/or the objective lens 224 may be driven by the driving device 30 to move to a target position to perform a focusing operation. Specifically, the driving device 30 may include a driving circuit and a stepping motor.
It should be understood that the microscope 22 may also include any other components known to those skilled in the art, such as, but not limited to, an eyepiece, a light source lamp, a base, etc.
In practice, for convenience of disassembly and assembly and maintenance, the objective lens 224 is usually kept still, and the stage 222 is driven by the driving device 30 to move, so that the stage 222 drives the counting cell 10 to move, thereby completing focusing. Throughout this document, the driving device 30 is used to drive the stage 222 to move, and in order to unnecessarily obscure the present invention, the connection manner and the driving process are simply described as: the driving device 30 is connected to the counting cell 10 and is used for driving the counting cell 10 to move so as to focus the urine sample.
The camera unit 24 is mounted above the objective 224 of the microscope 22 for taking images of the amplified urine sample in the field of view to obtain multiple images of the urine sample. The image capturing unit 24 may include any image capturing unit known in the art, such as various types of CCD cameras, CMOS cameras, and the like, which is not limited in the present invention.
The control means 40 is coupled to the drive means 30 for detecting the direction of movement of the cell 10 and for controlling the manner of movement of the cell 10. The movement mode of the counting cell 10 may include a movement direction, a movement distance, a delay movement time, and the like of the counting cell 10, which is not limited in the present invention. Specifically, the control device 40 transmits the control information to the driving device 30, and the driving device 30 drives the counting chamber 10 to move according to the control information, so as to focus the urine sample to be tested.
Specifically, when it is detected that the counting cell 10 moves to the target position in a second direction opposite to the preset first direction, the control device 40 controls the driving device 30 to move the counting cell 10 to a position beyond the target position by a predetermined distance in the second direction, and then drives the counting cell 10 to move to the target position in the first direction for focusing. Illustratively, the target position may be a focal plane position.
Referring to FIG. 2, FIG. 2 illustrates a schematic diagram of an exemplary path for driving the motion of the counting cell to eliminate the return difference, according to one embodiment of the invention. As shown in fig. 2, the direction of upward movement is preset to be a positive direction. If the starting point of the counting cell is at the position O and the counting cell is to move to the target position P, the moving direction of the counting cell is opposite to the positive direction, and the counting cell can be driven to move to a position exceeding the target position P by a preset distance X in the downward direction and then driven to move to the target position P in the upward direction. The predetermined distance X may also be referred to as an anti-backlash distance.
When it is detected that the counting cell 10 moves to the target position along the preset first direction, the control device 40 can control the driving device 30 to drive the counting cell 10 to move directly to the target position for focusing.
Still referring to FIG. 2, if the starting point of the counting cell is at position S, which is to be moved to the target position P, the direction of movement of the counting cell is in the positive direction, at which time the counting cell can be driven to move directly from position S to the target position P.
In practical use, the movement of the counting cell 10 may include 3 dimensions, i.e., an X direction, a Y direction and a Z direction, wherein the X direction may be a left-right direction, the Y direction may be a front-back direction, and the Z direction may be an up-down direction, i.e., a focusing direction. Wherein, the X, Y-directional movement of the counting cell 10 mainly ensures the accuracy of cell tracking and positioning, and the Z-directional movement ensures the clarity of cell shooting. The control device 40 of the embodiment of the present invention may control the movement of the counting cell 10 in any one or more of the X direction, the Y direction and the Z direction.
In practical application, the return stroke difference in the Z direction is about 0 to 7 micrometers, and the depth of field of the objective lens is also about several micrometers (for example, the depth of field of the 40-fold objective lens is only 1.3 micrometers), so that the influence of the movement in the Z direction on the definition of the shot picture is large, the shot picture is not clear due to the return stroke difference, and the definition directly influences the segmentation recognition and user experience of cells, so that the elimination of the return stroke difference in the movement in the Z direction has an important meaning. The control device 40 controls the movement of the counting cell 10 in the Z direction to perform focusing.
Illustratively, the first direction may be preset to a direction in which the counting cell 10 moves toward the objective lens of the image capture device 20 or away from the objective lens, i.e., may be preset to a + Z direction or a-Z direction.
In practical application, when the counting cell moves, the movement in the X direction, the Y direction and the Z direction can be simultaneous, and the movement of eliminating the return difference in the Z direction can affect the detection speed of the whole urine detection device, so that the performance index of the whole urine detection device is affected, and the urine detection device does not meet the production requirement, so that the movement time in the Z direction after the movement of the return difference is added does not exceed the movement time in the X, Y direction, so as to avoid the movement of eliminating the return difference from affecting the detection speed of the whole urine detection device. For example, the predetermined distance may be preset based on the return difference of the movement of the counting cell 10, and the detection speed of the urine sample detection device is not affected.
Through experiments, when the return distance difference is larger than 40 micrometers, the time of the Z-direction movement exceeds the movement time of the X, Y direction, and the influence on the movement speed of the whole machine is generated. While the return difference of the cell 10 in the Z direction is about 0-7 microns, the return difference distance should be larger than the return difference, and thus, for example, the predetermined distance (i.e., the return difference distance) may be 7-40 microns. Preferably, the predetermined distance should be selected as large as possible to ensure complete cancellation of the return difference, and thus the predetermined distance may be about 37 microns. It will be appreciated that in practice, the distances are typically expressed in steps of the stepper motor movement, for example the return difference is around 50 steps, and the predetermined distance may be around 150 steps.
In practical applications, due to inertia, after the control device 40 controls the driving device 30 to move the counting cell 10 to a predetermined distance beyond the target position in the second direction and a time delay interval elapses, the control device 40 controls the driving device 30 to drive the counting cell 10 to move to the target position in the first direction for focusing. The delay time interval may be set empirically, such as 0-200 ms, and the like, but the invention is not limited thereto.
According to the urine sample detection device of the embodiment, the return stroke difference of the focusing process of the microscope during urine sample detection is eliminated, the definition of a shot picture is improved, the detection speed of the detection device is not influenced, and only the urine sample detection flow is optimized, so that the cost is not increased.
Example two
The present embodiments provide a urine sample testing device. Referring to fig. 3, fig. 3 shows a schematic block diagram of a urine sample testing device 200 according to an embodiment of the present invention. As shown in fig. 3, the urine sample testing device 200 may be any urine sample testing device known in the art, such as a urine visible component analyzer, a urinary sediment analyzer, etc., but the present invention is not limited thereto. Specifically, the urine sample testing device may include a setup module 210, a control module 230, and the like. Wherein:
the setup module 210 may be used to preset a first direction of motion of a counting cell of a urine sample testing device. The counting cell is used for carrying a urine sample to be tested, and the urine sample may contain various tangible components, such as red blood cells, white blood cells, leukocyte aggregates, bacteria, epithelial cells, crystals, casts, mucus filaments, sperms, and the like, and may further contain various impurities. In addition, the counting cell is also used for providing a visual field for shooting.
For example, the first direction may be preset to a direction in which the counting cell moves toward or away from the objective lens of the image capture device, i.e., may be preset to a + Z direction or a-Z direction.
It should be understood that, during the use, the counting cell may be driven to move for focusing, the objective lens of the urine sample detection device 200 may also be driven to move for focusing, and both the counting cell and the objective lens may also be driven to move for focusing, which is not limited by the present invention. However, in practice, for convenience of disassembly and assembly and maintenance, the objective lens is usually kept still, and the counting cell is driven to move, so as to complete focusing. Therefore, the present embodiment is described by taking the example of driving the counting chamber to move for focusing.
The control module 230 is used for detecting the moving direction of the counting cell and controlling the moving mode of the counting cell. The movement mode of the counting cell may include a movement direction, a movement distance, a delay movement time, and the like of the counting cell, which is not limited in the present invention.
Specifically, when the counting pool is detected to move to the target position along a second direction opposite to the first direction, the counting pool is controlled to move to a position exceeding the target position by a preset distance along the second direction, and then the counting pool is controlled to move to the target position along the first direction. The predetermined distance may also be referred to as an anti-backlash distance.
For example, the predetermined distance may be preset based on the return difference of the movement of the counting cell, and the detection speed of the urine sample detection device is not affected.
Illustratively, the predetermined distance (i.e., the return path difference elimination distance) may be 7-40 microns. Preferably, the predetermined distance should be selected as large as possible to ensure complete cancellation of the return difference, and thus the predetermined distance may be about 37 microns. It will be appreciated that in practice, the distances are typically expressed in steps of the stepper motor movement, for example the return difference is around 50 steps, and the predetermined distance may be around 150 steps.
In practical application, due to inertia, after the control module controls the counting cell to move to a position exceeding a target position by a preset distance along the second direction and a delay time interval, the control module controls the counting cell to move to the target position along the first direction for focusing. The delay time interval may be set empirically, such as 0-200 ms, and the like, but the invention is not limited thereto.
According to the urine sample detection device of the embodiment, the return stroke difference of the focusing process of the microscope during urine sample detection is eliminated, the definition of a shot picture is improved, the detection speed of the detection device is not influenced, and only the urine sample detection flow is optimized, so that the cost is not increased.
EXAMPLE III
The embodiment provides a urine sample detection method which is applied to a urine sample detection device. The urine sample detection device may be any urine sample detection device known in the art, such as a urine visible component analyzer, a urinary sediment analyzer, and the like, which is not limited in this respect. Wherein, urine sample check out test set can include image acquisition device for enlarge and shoot the urine sample in shooting visual field district, in order to obtain many images.
Referring to fig. 4, fig. 4 illustrates an exemplary flow chart of a urine sample testing method 400 according to one embodiment of the invention. Specifically, the urine sample detection method 400 may include:
step S410: the counting cell is driven to move so as to focus the urine sample, and the moving direction of the counting cell is detected.
The counting cell is used for carrying a urine sample to be tested, and the urine sample may contain various tangible components, such as red blood cells, white blood cells, leukocyte aggregates, bacteria, epithelial cells, crystals, casts, mucus filaments, sperms, and the like, and may further contain various impurities. In addition, the counting cell is also used for providing a visual field for shooting. Because each visible component in the urine sample can be layered, a certain visible component in the urine sample can be focused and photographed to obtain a clear image.
It should be understood that, during the use, the counting cell may be driven to move to the target position for focusing, the objective lens of the image analysis device may also be driven to move for focusing, and both the counting cell and the objective lens may also be driven to move simultaneously for focusing, which is not limited by the present invention. However, in practice, for convenience of disassembly and assembly and maintenance, the objective lens is usually kept still, and the counting cell is driven to move, so as to complete focusing. Therefore, the present embodiment is described by taking the example of driving the counting chamber to move for focusing.
Specifically, when the counting cell is detected to move to the target position along a second direction opposite to the preset first direction, the counting cell is driven to move to a position exceeding the target position by a preset distance along the second direction, and then the counting cell is driven to move to the target position along the first direction for focusing. Illustratively, the target position may be a focal plane position. The predetermined distance may also be referred to as an anti-backlash distance.
For example, the first direction may be preset to a direction in which the counting cell moves toward or away from the objective lens of the image capture device, i.e., may be preset to a + Z direction or a-Z direction.
For example, the predetermined distance (i.e., the cancellation return difference distance) may be predetermined based on the return difference of the movement of the counting cell, and may not affect the detection speed of the urine sample detection device. Illustratively, the predetermined distance may be 7-40 microns. Preferably, the predetermined distance should be selected as large as possible to ensure complete cancellation of the return difference, and thus the predetermined distance may be about 37 microns. It will be appreciated that in practice, the distances are typically expressed in steps of the stepper motor movement, for example the return difference is around 50 steps, and the predetermined distance may be around 150 steps.
When the counting cell is detected to move towards the target position along the preset first direction, the counting cell can be driven to directly move to the target position for focusing.
In practical application, due to inertia, the counting cell can be driven to move to a position exceeding a target position by a predetermined distance along the second direction, and then the counting cell can be driven to move to the target position along the first direction for focusing after a delay time interval. The delay time interval may be set empirically, such as 0-200 ms, and the like, but the invention is not limited thereto.
Step S420: and controlling a camera unit of the image acquisition device to photograph the focused urine sample in the counting cell so as to acquire a plurality of images of the urine sample.
Illustratively, the urine specimen may be photographed using any camera unit known in the art, such as various types of CCD cameras, CMOS cameras, and the like. For example, the acquired image may be used to analyze various tangible components in the urine sample, and may be subjected to a series of processes to obtain clear images of various tangible components, respectively.
According to the urine sample detection method, the return stroke difference of the microscope in the focusing process during urine sample detection is eliminated, the definition of the shot pictures is improved, and only the urine sample detection flow is optimized, so that the cost is not increased.
Two exemplary application examples of the urine sample detection method of the present embodiment are described in detail below.
Firstly, the urine sample detection method can be used for the focal length compensation process during switching of high and low power objective lenses in the urine sample detection process.
When the microscope is switched from the low-power lens to the high-power lens, because the objective lens has a parameter, namely the parfocal distance, which is the distance from the focal plane to the rear section of the objective lens when the objective lenses with different magnifications are imaged, the parfocal distance is the most ideal condition, when the objective lens with a certain magnification is successfully focused, the objective lens is switched to the objective lens with different magnifications, the focal distance is not required to be adjusted, but the operation is impossible, for example, a sample under the low-power lens cannot be found or a clear image cannot be obtained after the objective lens is switched from the low-power lens to the high-power lens, which is related to the focal depth of the objective lens. In order to obtain a clear image, when the high-power mirror and the low-power mirror are switched with each other, the focal length needs to be readjusted in the positive direction or the negative direction of the Z axis, and at this time, the method of the embodiment can be adopted to eliminate the influence of the return path difference.
Secondly, the urine sample detection method can be used in the focus compensation process when moving from one shooting area to another shooting area in the urine sample detection process.
When using the sediment analyzer to detect the tangible composition in the urine, at first acquire the urine sample, in passing through the counting cell on the microscope objective table with the urine sample provides the device input microscope objective, close the injection valve of urine providing device after the counting cell inner chamber is full of the urine sample that awaits measuring, the urine sample that awaits measuring is static and rely on the effect of gravity to make tangible composition such as cell subside to the slide under the counting cell, and evenly distributed is in the counting cell to make tangible composition such as cell have unanimous plane of focusing for microscope objective.
At present, an automatic focusing manner is usually adopted to focus a shooting area where a urine sample in a counting chamber is located, specifically, referring to fig. 5, a mark pattern is etched on a cavity or a surface of the counting chamber, and a first mark pattern 101 is generally set on a first side of the cavity or the surface of the counting chamber, and a second mark pattern 102 is generally set on a second side of the cavity or the surface of the counting chamber. Preferably, the ideal shooting area 103 is 0.2-0.6 cm away from the first mark pattern 101, 0.2-0.6 cm away from the second mark pattern 102, and 0.2-0.6 cm away from the upper and lower boundaries of the counting cell plane, and the obtained ideal shooting area 103 can avoid the interference of uneven lighting, shadow phenomena and mark patterns in the boundary area, and meanwhile, the enough area of the shooting area can be ensured. The shape of the mark pattern etched on the counting chamber or the surface can be a "#" pattern, and also can be other figures, symbols, characters or the combination thereof. Before the shooting area is shot, the mark pattern is shot according to the default focal length, the focal length variation is calculated according to the image definition value and the definition calibration curve, the distance between the mark pattern and the urine sample to be detected is constant, so that the actual focal length is indirectly calculated, then the shooting is carried out according to the actual focal length, then the image definition is evaluated, and the automatic focusing of a microscope is realized.
After shooting with the low power lens, the shooting area is composed of a plurality of (e.g., 160) consecutive minimum shooting areas with the same size, when shooting with the high power lens is changed, a plurality of (e.g., 10) areas in the minimum shooting areas are selected for shooting, because the surface of the counting cell is uneven, the Z axis is compensated, and the Z axis compensation of each small shooting area is different, when moving from one small shooting area to another small shooting area, the focal length needs to be finely adjusted again along the Z axis direction, the focal length can be adjusted along the positive Z axis direction, or the focal length can be adjusted along the negative Z axis direction, and at this time, the method of the embodiment can be adopted to eliminate the influence of the return distance difference.
Similarly, when shooting is performed by using the low power lens, when the shooting field of view needs to be moved from one position of the shooting area to another position, because the surface of the counting cell is uneven, the Z axis has compensation, and the Z axis compensation at each position of the shooting area is different, the focal length needs to be re-adjusted along the Z axis direction, and also can be adjusted along the positive direction of the Z axis, or adjusted along the negative direction of the Z axis, and at this time, the method of the embodiment can be adopted to eliminate the influence of the return distance difference.
It should be understood that the method of the present invention can also be applied to other situations where it is necessary to eliminate the return difference during the urine sample detection process, for example, the movement process of a syringe, an injector, etc., and the present invention is not limited thereto.
According to the urine sample detection method, the return stroke difference of the microscope in the focusing process during urine sample detection is eliminated, the definition of a shot picture is improved, the detection speed of detection equipment is not influenced, the urine sample detection process is only optimized, and the cost is not increased.
Example four
The present embodiment provides a computer-readable medium having stored thereon a computer program which, when executed, performs the method as described in the above embodiments. Any tangible, non-transitory computer-readable medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-ROMs, DVDs, Blu-ray disks, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means for implementing the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.
The invention has the technical effects that:
the scheme of the invention eliminates the return stroke difference of the microscope in the focusing process during the urine sample detection, improves the definition of the shot picture, does not influence the detection speed of the detection equipment, only optimizes the urine sample detection flow, and can improve the performance of all on-sale instruments by the method of the invention without increasing the cost.
Although the illustrative embodiments have been described herein with reference to the accompanying drawings, it is to be understood that the foregoing illustrative embodiments are merely exemplary and are not intended to limit the scope of the invention thereto. Various changes and modifications may be effected therein by one of ordinary skill in the pertinent art without departing from the scope or spirit of the present invention. All such changes and modifications are intended to be included within the scope of the present invention as set forth in the appended claims.
In the description provided herein, numerous specific details are set forth. It is understood, however, that embodiments of the invention may be practiced without these specific details. In some instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description.
Similarly, it should be appreciated that in the description of exemplary embodiments of the invention, various features of the invention are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the invention and aiding in the understanding of one or more of the various inventive aspects. However, the method of the present invention should not be construed to reflect the intent: that the invention as claimed requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this invention.
It will be understood by those skilled in the art that all of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and all of the processes or elements of any method or apparatus so disclosed, may be combined in any combination, except combinations where such features are mutually exclusive. Each feature disclosed in this specification (including any accompanying claims, abstract and drawings) may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise.
Furthermore, those skilled in the art will appreciate that while some embodiments described herein include some features included in other embodiments, rather than other features, combinations of features of different embodiments are meant to be within the scope of the invention and form different embodiments. For example, in the claims, any of the claimed embodiments may be used in any combination.
It should be noted that the above-mentioned embodiments illustrate rather than limit the invention, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, any reference signs placed between parentheses shall not be construed as limiting the claim. The word "comprising" does not exclude the presence of elements or steps not listed in a claim. The word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements. The invention may be implemented by means of hardware comprising several distinct elements, and by means of a suitably programmed computer. In the unit claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The usage of the words first, second and third, etcetera do not indicate any ordering. These words may be interpreted as names.
The above description is only for the specific embodiment of the present invention or the description thereof, and the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and the changes or substitutions should be covered within the protection scope of the present invention. The protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (16)

1. The utility model provides a urine sample check out test set which characterized in that, urine sample check out test set includes counting cell, image acquisition device, drive arrangement and controlling means, wherein:
the counting cell is used for bearing a urine sample to be tested;
the image acquisition device comprises a camera unit, wherein the camera unit is used for shooting the urine sample in a visual field area so as to acquire a plurality of images of the urine sample;
the driving device is connected to the counting cell and used for driving the counting cell to move so as to focus the urine sample;
the control device is coupled to the driving device and used for detecting the movement direction of the counting cell and controlling the movement mode of the counting cell, wherein when the counting cell is detected to move towards the target position along a second direction opposite to the preset first direction, the control device controls the driving device to actuate the counting cell to move to a position exceeding the target position by a preset distance along the second direction and then drive the counting cell to move to the target position along the first direction for focusing.
2. The urine sample testing device according to claim 1, wherein said first direction comprises a direction in which said counting cell moves towards or away from an objective lens of said image acquisition apparatus.
3. The urine sample testing device according to claim 1, wherein after a time delay interval has elapsed after the control means controls the driving means to move the counting cell in the second direction to a predetermined distance beyond the target position, the control means controls the driving means to move the counting cell in the first direction to the target position for focusing.
4. The urine sample testing device according to claim 1, wherein when it is detected that the counting cell moves in the first direction to the target position, the control means controls the driving means to drive the counting cell to move directly to the target position for focusing.
5. The urine sample testing device according to claim 1, wherein the predetermined distance is preset based on a return difference of the movement of the counting cell without affecting the testing speed of the urine sample testing device.
6. The urine sample testing device according to any of claims 1-5, wherein said target position comprises a focal plane position.
7. A urine sample testing device, comprising:
the setting module is used for presetting a first direction of movement of a counting cell of the urine sample detection equipment; and
and the control module is used for detecting the movement direction of the counting pool and controlling the movement mode of the counting pool, wherein when the counting pool is detected to move to a target position along a second direction opposite to the first direction, the counting pool is firstly controlled to move to a position exceeding the target position by a preset distance along the second direction, and then the counting pool is controlled to move to the target position along the first direction.
8. The urine sample detection method is applied to urine sample detection equipment, the urine sample detection equipment comprises an image acquisition device, and the urine sample detection method is characterized by comprising the following steps of:
driving the counting cell to move to focus the urine sample, and detecting the moving direction of the counting cell, wherein:
when the counting cell is detected to move to a target position along a second direction opposite to the preset first direction, the counting cell is firstly driven to move to a position exceeding the target position by a preset distance along the second direction, and then the counting cell is driven to move to the target position along the first direction for focusing;
and controlling a camera unit of the image acquisition device to photograph the focused urine sample in the counting cell so as to acquire a plurality of images of the urine sample.
9. The urine sample testing method according to claim 8, wherein said first direction comprises a direction in which said counting cell moves toward or away from an objective lens of said image capturing device.
10. The urine sample test method according to claim 8, further comprising: and after a time delay time interval is passed after the counting cell is driven to move to a position exceeding the target position along the second direction in advance, the counting cell is driven to move to the target position along the first direction for focusing.
11. The urine sample test method according to claim 8, further comprising: when the counting pool is detected to move to the target position along the first direction, the counting pool is driven to directly move to the target position for focusing.
12. The urine sample test method according to claim 8, wherein the predetermined distance is preset based on a return difference of the movement of the counting cell without affecting the test speed of the urine sample test apparatus.
13. The urine sample testing method according to any of claims 8 to 12, wherein said target position comprises a focal plane position.
14. The urine sample test method according to any one of claims 8 to 12, wherein the urine sample test method is used for a focal length compensation process when switching the high-power and low-power objective lenses in the urine sample test process.
15. The urine sample test method according to any one of claims 8 to 12, wherein the urine sample test method is used for a focus compensation process when moving from one photographing region to another photographing region in a urine sample test process.
16. A computer-readable storage medium having computer-executable instructions stored thereon that, when executed by a processor, cause the processor to perform operations comprising:
presetting a first direction of movement of a counting cell of urine sample detection equipment, and detecting the movement direction of the counting cell;
when the counting cell is detected to move to the target position along a second direction opposite to the first direction, the counting cell is moved to a position exceeding the target position by a preset distance along the second direction, and then the counting cell is driven to move to the target position along the first direction for focusing.
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