CN113046924A - 一种静电纺丝制备小直径血管支架的方法 - Google Patents

一种静电纺丝制备小直径血管支架的方法 Download PDF

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CN113046924A
CN113046924A CN201911374285.4A CN201911374285A CN113046924A CN 113046924 A CN113046924 A CN 113046924A CN 201911374285 A CN201911374285 A CN 201911374285A CN 113046924 A CN113046924 A CN 113046924A
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赵妙妙
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Abstract

本发明一种静电纺丝制备小直径血管支架的方法涉及血管支架制备领域，具体涉及一种静电纺丝制备小直径血管支架的方法，包括以下步骤：配制纺丝液，以甲酸为溶液，配制质量百分比为2%的RGD‑重组蛛丝蛋白溶液和25%的聚己内酯溶液及5%的壳聚糖溶液；本发明利用聚合物溶液或熔体在强电场作用下形成喷射流，射流凝固形成纳米纤维，以无纺布状排列在接收轴柄上，操作方法简单，本发明采用静电纺丝构建RGD‑重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液复合纳米纤维小直径血管支架，该血管支架具有三维多孔网状结构，能够模拟细胞外基质的结构和功能，这种结构有利于种子细胞在支架上的黏附、生长和迁移。

Description

一种静电纺丝制备小直径血管支架的方法
技术领域
本发明涉及血管支架制备领域，具体涉及一种静电纺丝制备小直径血管支架的方法。
背景技术
人工合成血管和自体血管是目前临床上小直径(<6mm)血管移植物的主要来源。在实践中人工合成血管存在植入后容易造成血栓形成、内膜增生、远期通畅率不高等问题；自体血管受到供体来源、直径及长度等限制，还无法满足小直径血管移植物的供给需求。组织工程血管为解决小直径血管移植物来源提供了有效的途径，制备可供细胞进行生命活动的支架并对支架材料进行生物学方面的评价是组织工程血管研究的重要领域。近年，组织工程血管支架材料研究的重点是将不同材料按一定的方法组合构建为复合材料，如明胶／弹力蛋白／聚丙交酯-乙交酯共聚物、丝素蛋白／聚乳酸、胶原／聚己内酯等，以发挥材料各自的优点。
根据蜘蛛丝的结构和功能特性，通过基因工程技术方法制备重组蛛丝蛋白，并在蛛丝蛋白的功能重复基因序列中引入RGD(精氨酸-甘氨酸-天冬氨酸)三肽密码子，构建并表达了RGD-重组蛛丝蛋白，生物活性分子RGD三肽是粘附蛋白中能够为细胞表面特异受体蛋白识别的最小短肽序列，是目前应用最广、最有效的促黏附肽。聚己内酯具有良好的力学性能和柔韧性而壳聚糖则有优良的生物相容性和可降解性。
发明内容
本发明目的在于提供一种静电纺丝制备小直径血管支架的方法，该血管支架具有三维多孔网状结构，能够模拟细胞外基质的结构和功能，这种结构有利于种子细胞在支架上的黏附、生长和迁移。
本发明一种静电纺丝制备小直径血管支架的方法，包括以下步骤：
第一步，配制纺丝液，以甲酸为溶液，配制质量百分比为2%的RGD-重组蛛丝蛋白溶液和25%的聚己内酯溶液及5%的壳聚糖溶液，然后将RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液混合制备得到混合纺丝液；
第二步，电纺，吸取1.5ml的混合纺丝液至规格为2ml的注射器中，将注射器安装至注射泵上，接收装置为转动设置的接收轴柄，针头与地线相连，接收轴柄与高压电源相连；
开启注射泵及高压电源，在静电场作用下，混合纺丝液在飞向接收轴柄过程中被拉伸，随着溶剂的挥发，最终形成复合纳米纤维的小直径血管支架。
优选地，纺丝液中RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液的质量比为5:85:10。
优选地，纺丝过程参数设定为：电压80kV，固化距离20cm，挤出速度为5ml/h，温度为45摄氏度。
优选地，接收轴柄上涂覆有润滑剂。
优选地，润滑剂为乙二醇。
本发明利用聚合物溶液或熔体在强电场作用下形成喷射流，射流凝固形成纳米纤维，以无纺布状排列在接收轴柄上，操作方法简单，本发明采用静电纺丝构建RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液复合纳米纤维小直径血管支架，该血管支架具有三维多孔网状结构，能够模拟细胞外基质的结构和功能，这种结构有利于种子细胞在支架上的黏附、生长和迁移。
具体实施方式
实施例一
本发明一种静电纺丝制备小直径血管支架的方法，包括以下步骤：
第一步，配制纺丝液，以甲酸为溶液，配制质量百分比为2%的RGD-重组蛛丝蛋白溶液和25%的聚己内酯溶液及5%的壳聚糖溶液，然后将RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液混合制备得到混合纺丝液；
第二步，电纺，吸取1.5ml的混合纺丝液至规格为2ml的注射器中，将注射器安装至注射泵上，接收装置为转动设置的接收轴柄，针头与地线相连，接收轴柄与高压电源相连；
开启注射泵及高压电源，在静电场作用下，混合纺丝液在飞向接收轴柄过程中被拉伸，随着溶剂的挥发，最终形成复合纳米纤维的小直径血管支架。
纺丝液中RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液的质量比为5:85:10。
纺丝过程参数设定为：电压80kV，固化距离20cm，挤出速度为5ml/h，温度为45摄氏度。
接收轴柄上涂覆有润滑剂。润滑剂为乙二醇。
实施例二
本发明一种静电纺丝制备小直径血管支架的方法，包括以下步骤：
第一步，配制纺丝液，以甲酸为溶液，配制质量百分比为2%的RGD-重组蛛丝蛋白溶液和25%的聚己内酯溶液及5%的壳聚糖溶液，然后将RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液混合制备得到混合纺丝液；纺丝液中RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液的质量比为5:85:10。
第二步，电纺，吸取1.5ml的混合纺丝液至规格为2ml的注射器中，将注射器安装至注射泵上，接收装置为转动设置的接收轴柄，针头与地线相连，接收轴柄与高压电源相连；纺丝过程参数设定为：电压80kV，固化距离20cm，挤出速度为5ml/h，温度为45摄氏度。
开启注射泵及高压电源，在静电场作用下，混合纺丝液在飞向接收轴柄过程中被拉伸，随着溶剂的挥发，最终形成复合纳米纤维的小直径血管支架。
接收轴柄上涂覆有润滑剂。润滑剂为乙二醇。
本发明利用聚合物溶液或熔体在强电场作用下形成喷射流，射流凝固形成纳米纤维，以无纺布状排列在接收轴柄上，操作方法简单，本发明采用静电纺丝构建RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液复合纳米纤维小直径血管支架，该血管支架具有三维多孔网状结构，能够模拟细胞外基质的结构和功能，这种结构有利于种子细胞在支架上的黏附、生长和迁移。

Claims (5)

1.一种静电纺丝制备小直径血管支架的方法，其特征在于，包括以下步骤：
第一步，配制纺丝液，以甲酸为溶液，配制质量百分比为2%的RGD-重组蛛丝蛋白溶液和25%的聚己内酯溶液及5%的壳聚糖溶液，然后将RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液混合制备得到混合纺丝液；
第二步，电纺，吸取1.5ml的混合纺丝液至规格为2ml的注射器中，将注射器安装至注射泵上，接收装置为转动设置的接收轴柄，针头与地线相连，接收轴柄与高压电源相连；
开启注射泵及高压电源，在静电场作用下，混合纺丝液在飞向接收轴柄过程中被拉伸，随着溶剂的挥发，最终形成复合纳米纤维的小直径血管支架。
2.如权利要求1所述一种静电纺丝制备小直径血管支架的方法，其特征在于，所述纺丝液中RGD-重组蛛丝蛋白溶液、聚己内酯溶液及壳聚糖溶液的质量比为5:85:10。
3.如权利要求2所述一种静电纺丝制备小直径血管支架的方法，其特征在于，纺丝过程参数设定为：电压80kV，固化距离20cm，挤出速度为5ml/h，温度为45摄氏度。
4.如权利要求3所述一种静电纺丝制备小直径血管支架的方法，其特征在于，所述接收轴柄上涂覆有润滑剂。
5.如权利要求4所述一种静电纺丝制备小直径血管支架的方法，其特征在于，所述润滑剂为乙二醇。
CN201911374285.4A 2019-12-27 2019-12-27 一种静电纺丝制备小直径血管支架的方法 Pending CN113046924A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114949362A (zh) * 2022-07-29 2022-08-30 海迈医疗科技(苏州)有限公司 一种体内快速再细胞化组织工程血管及其制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114949362A (zh) * 2022-07-29 2022-08-30 海迈医疗科技(苏州)有限公司 一种体内快速再细胞化组织工程血管及其制备方法
CN114949362B (zh) * 2022-07-29 2022-10-28 海迈医疗科技(苏州)有限公司 一种体内快速再细胞化组织工程血管及其制备方法

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