CN113025513A - A health powder for preventing scytitis and its preparation method - Google Patents
A health powder for preventing scytitis and its preparation method Download PDFInfo
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- CN113025513A CN113025513A CN201911352405.0A CN201911352405A CN113025513A CN 113025513 A CN113025513 A CN 113025513A CN 201911352405 A CN201911352405 A CN 201911352405A CN 113025513 A CN113025513 A CN 113025513A
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- powder
- lactobacillus acidophilus
- submicron
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- 239000000843 powder Substances 0.000 title claims abstract description 52
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 230000036541 health Effects 0.000 title description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 31
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 31
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 31
- 241000894006 Bacteria Species 0.000 claims abstract description 20
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims abstract description 17
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 7
- 229920002472 Starch Polymers 0.000 claims abstract description 7
- 235000019359 magnesium stearate Nutrition 0.000 claims abstract description 7
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 7
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 7
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 7
- 239000008107 starch Substances 0.000 claims abstract description 7
- 235000019698 starch Nutrition 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 4
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000010410 dusting Methods 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 201000004624 Dermatitis Diseases 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 2
- 230000004054 inflammatory process Effects 0.000 abstract description 2
- 206010040872 skin infection Diseases 0.000 abstract description 2
- 210000003491 skin Anatomy 0.000 description 9
- 239000006041 probiotic Substances 0.000 description 7
- 230000000529 probiotic effect Effects 0.000 description 7
- 235000018291 probiotics Nutrition 0.000 description 7
- 238000007710 freezing Methods 0.000 description 5
- 230000008014 freezing Effects 0.000 description 5
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 238000011049 filling Methods 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007366 host health Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000006872 mrs medium Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000001393 triammonium citrate Substances 0.000 description 1
- 235000011046 triammonium citrate Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/732—Starch; Amylose; Amylopectin; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q15/00—Anti-perspirants or body deodorants
Abstract
The invention discloses lactobacillus acidophilus LA1063 (aLactobacillus acidophilus) The talcum powder using the lactobacillus acidophilus LA1063 and the preparation method of the talcum powder comprise the following components in percentage by mass: 95-99% of starch and 1-5% of submicron (300-; wherein the submicron bacterium powder comprises 89.5-94.5% of Lactobacillus acidophilus LA1063 powder, 5-10% of microcrystalline cellulose and 0.5-1% of magnesium stearate. The talcum powder of the invention can be directly smearedOn the surface of the intact skin, the effects of reducing the incidence of skin infection and inflammation and improving bad body taste are achieved.
Description
Technical Field
The invention relates to active probiotic talcum powder for preventing skin inflammation and a preparation method thereof.
Background
Human skin has a transient, temporary indigenous flora containing epidermal bacteria, some of which are beneficial to health, as intestinal bacteria are essential to the health of the host. The skin microecological flora uses skin lipid as a nutrient, maintains skin acidity, and prevents other microorganisms from growing on the skin. Most microorganisms are gram-positive, residing on the skin surface and in hair follicles. Hosts have a range of structures, molecules and mechanisms to confine these temporary and transient occupants, and also to control their population and to predominate certain microorganisms. The indigenous flora occupying a niche is beneficial to the host and may be harmful or infectious when moved about. Therefore, the development of a talcum powder for preventing skin inflammation is urgently needed to meet the market demand.
Disclosure of Invention
In order to solve the problem that flora is possibly harmful or infected to skin, the invention provides lactobacillus acidophilus LA1063, active probiotic talcum powder using the lactobacillus acidophilus LA1063 and capable of preventing skin inflammation and a preparation method of the talcum powder. The technical scheme of the invention is as follows.
Lactobacillus acidophilus LA1063 (A)Lactobacillus acidophilus) The strain is deposited in the China general microbiological culture Collection center on 28 th 10 th 2019 month, and the preservation number is CGMCC number 18744.
The 16S rDNA sequence of the lactobacillus acidophilus LA1063 has a sequence structure shown in SEQ ID No. 1.
The sequence structure of SEQ ID NO.1 is as follows:
GGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTGAACCAACAGATTCACTTCGGTGATGACGTTGGGAACGCGAGCGGCGGATGGGTGAGTAACACGTGGGGAACCTGCCCCATAGTCTGGGATACCACTTGGAAACAGGTGCTAATACCGGATAAGAAAGCAGATCGCATGATCAGCTTATAAAAGGCGGCGTAAGCTGTCGCTATGGGATGGCCCCGCGGTGCATTAGCTAGTTGGTAGGGTAACGGCCTACCAAGGCAATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTGGTGAAGAAGGATAGAGGTAGTAACTGGCCTTTATTTGACGGTAATCAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGAAGAATAAGTCTGATGTGAAAGCCCTCGGCTTAACCGAGGAACTGCATCGGAAACTGTTTTTCTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAAGTGTTGGGAGGTTTCCGCCTCTCAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTAGTGCAATCCGTAGAGATACGGAGTTCCCTTCGGGGACACTAAGACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCAGCATTAAGTTGGGCACTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGTACAACGAGGAGCAAGCCTGCGAAGGCAAGCGAATCTCTTAAAGCTGTTCTCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGCACGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCTGCAATGCCCAAAGCCGGTGGCCTAACCTTCGGGAAGGAGCCGTCTAAGGCAGGGCAGATGACTGGGGTGAAGTC。
the talcum powder using the lactobacillus acidophilus LA1063 comprises the following components in percentage by mass: 95-99% of starch and 1-5% of submicron (300 meshes and 500 meshes) bacterial powder; wherein the submicron bacterium powder comprises 89.5-94.5% of Lactobacillus acidophilus LA1063 powder, 5-10% of microcrystalline cellulose and 0.5-1% of magnesium stearate.
The talcum powder is suitable for intact skin.
The preparation method of the talcum powder comprises the following steps:
1) primary mixing: mixing the lactobacillus acidophilus LA1063 powder, the microcrystalline cellulose and the magnesium stearate in the proportion to obtain a bacterium powder mixture;
2) crushing: the bacterial powder mixture is crushed by supersonic airflow and passes through a sieve with the mesh number of 300-500 meshes to form submicron bacterial powder;
3) and (3) secondary mixing: and uniformly mixing the starch and the submicron bacterium powder in the proportion to obtain the cool raw powder.
In the step 2), the viable count of the submicron bacterium powder is 1 multiplied by 1010～1×1011cfu/g。
Freezing the mixture of the bacterial powder at-20 deg.C for over 80min before step 2).
Filling the talcum powder after the step 3).
Has the advantages that: the talcum powder can be directly smeared on the surface of intact skin, and plays roles in reducing the occurrence probability of skin infection and inflammation and improving bad body taste; the preparation method of the refreshing raw powder provided by the invention has the advantages that the probiotic bacteria powder raw material is crushed to 300-mesh 500-mesh powder, so that the problem that the probiotic bacteria powder is too coarse and is not suitable for preparing the refreshing raw powder or is too fine to damage lactic acid bacteria is avoided; by adopting the superfine grinding mode for grinding, the inactivation of lactic acid bacteria caused by a large amount of heat generated by conventional mechanical grinding can be avoided; the best effect is achieved by adopting a mode of freezing the bacterial powder and then carrying out airflow ultramicro and controlling a certain mesh number.
Detailed Description
The specific cultivation method of lactobacillus acidophilus LA1063 disclosed by the invention is as follows:
(1) preparation of MRS Medium
The MRS culture medium comprises the following specific components:
peptone 10.0 g
Beef powder 5.0 g
Yeast powder 4.0 g
Glucose 20.0 g
Tween 801.0 mL
K2HPO4·7H2O 2.0 g
Sodium acetate 3H2O 5.0.0 g
Triammonium citrate 2.0 g
MgSO4·7H2O 0.2 g
MnSO4·4H2O 0.05 g
Agar powder 15.0 g
pH 6.2
Method for producing
Adding the components into 1000mL of distilled water, heating to dissolve, adjusting the pH, subpackaging, and then carrying out autoclaving at 121 ℃ for 15-20 min.
(2) Lactobacillus acidophilus LA1063 isolated from milk products
Separating Lactobacillus acidophilus LA1063 from yogurt;
(3) diluting Lactobacillus acidophilus LA1063 with sterile physiological saline;
(4) 2 to 3 suitable dilutions (10) were selected-6、10-7、10-8) Respectively placing 0.1mL of the mixture on 2 MRS agar plates, performing surface coating by using an L-shaped rod, and performing anaerobic culture at 37 ℃ for 48 hours;
(5) picking the bacterial colony on the plate by using an inoculating loop, inoculating the bacterial colony to a fermentation medium (the specific composition of the fermentation medium is 25g/L of glucose, 10g/L of soybean peptone, 8g/L of yeast powder, 42 g/L of K2HPO, 7g/L of CH3COONa, 3g/L of sodium citrate, 40.2 g/L of MgSO40, 20.1g/L of FeCl20.1g/L of MnSO40.05g/L), culturing at medium-high density, and fermenting for 12 hours at the temperature of 37 ℃; and then centrifugally separating to obtain bacterial paste, adding a freezing protective agent, and carrying out freeze drying to obtain lactobacillus acidophilus LA1063 freeze-dried powder.
The lactobacillus acidophilus LA1063 disclosed by the invention is deposited in the China general microbiological culture Collection center on 28 th 10 th 2019, and the preservation number is CGMCC number 18744.
The lactobacillus acidophilus LA1063 is lactobacillus acidophilus LA 1063: (Lactobacillus acidophilus）LA1063。
Example 1
Mixing 89.5% of lactobacillus acidophilus LA1063 bacterial powder, 5% of microcrystalline cellulose and 0.5% of magnesium stearate in percentage by mass to obtain a bacterial powder mixture;
freezing the mixture in a freezer at-20 deg.C for 80 min;
supersonic jet pulverizing the frozen mixture of bacteria powder with supersonic jet mill, and sieving with 300 mesh sieve to obtain ultramicro bacteria powder with viable count of 1 × 1010～1×1011cfu/g；
Uniformly mixing 95% of starch and 1% of submicron bacterium powder to obtain active probiotic fresh powder 1 for preventing skin inflammation;
finally, filling the talcum powder 1.
Example 2
Mixing 94.5% of lactobacillus acidophilus LA1063 bacterial powder, 10% of microcrystalline cellulose and 1% of magnesium stearate in percentage by mass to obtain a bacterial powder mixture;
freezing the mixture in a freezer at-20 deg.C for 80 min;
supersonic jet pulverizing the frozen mixture of bacteria powder with supersonic jet mill, and sieving with 500 mesh sieve to obtain ultramicro bacteria powder with viable count of 1 × 1010～1×1011cfu/g；
Uniformly mixing 99% of starch and 5% of submicron bacterium powder to obtain active probiotic fresh powder 2 for preventing skin inflammation;
finally, filling the talcum powder 2.
As noted above, while the present invention has been shown and described with reference to certain preferred embodiments, it is not to be construed as limited thereto. Various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Sequence listing
<110> Biotechnology (Yangzhou) Ltd
<120> an active probiotic talcum powder for preventing skin inflammation and a preparing method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1498
<212> DNA
<213> Lactobacillus acidophilus (Lactobacillus acidophilus)
<400> 1
ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtcgagcgag ctgaaccaac 60
agattcactt cggtgatgac gttgggaacg cgagcggcgg atgggtgagt aacacgtggg 120
gaacctgccc catagtctgg gataccactt ggaaacaggt gctaataccg gataagaaag 180
cagatcgcat gatcagctta taaaaggcgg cgtaagctgt cgctatggga tggccccgcg 240
gtgcattagc tagttggtag ggtaacggcc taccaaggca atgatgcata gccgagttga 300
gagactgatc ggccacattg ggactgagac acggcccaaa ctcctacggg aggcagcagt 360
agggaatctt ccacaatgga cgaaagtctg atggagcaac gccgcgtgag tgaagaaggt 420
tttcggatcg taaagctctg ttgttggtga agaaggatag aggtagtaac tggcctttat 480
ttgacggtaa tcaaccagaa agtcacggct aactacgtgc cagcagccgc ggtaatacgt 540
aggtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg aagaataagt 600
ctgatgtgaa agccctcggc ttaaccgagg aactgcatcg gaaactgttt ttcttgagtg 660
cagaagagga gagtggaact ccatgtgtag cggtggaatg cgtagatata tggaagaaca 720
ccagtggcga aggcggctct ctggtctgca actgacgctg aggctcgaaa gcatgggtag 780
cgaacaggat tagataccct ggtagtccat gccgtaaacg atgagtgcta agtgttggga 840
ggtttccgcc tctcagtgct gcagctaacg cattaagcac tccgcctggg gagtacgacc 900
gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt 960
aattcgaagc aacgcgaaga accttaccag gtcttgacat ctagtgcaat ccgtagagat 1020
acggagttcc cttcggggac actaagacag gtggtgcatg gctgtcgtca gctcgtgtcg 1080
tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg tcattagttg ccagcattaa 1140
gttgggcact ctaatgagac tgccggtgac aaaccggagg aaggtgggga tgacgtcaag 1200
tcatcatgcc ccttatgacc tgggctacac acgtgctaca atggacagta caacgaggag 1260
caagcctgcg aaggcaagcg aatctcttaa agctgttctc agttcggact gcagtctgca 1320
actcgactgc acgaagctgg aatcgctagt aatcgcggat cagcacgccg cggtgaatac 1380
gttcccgggc cttgtacaca ccgcccgtca caccatggga gtctgcaatg cccaaagccg 1440
gtggcctaac cttcgggaag gagccgtcta aggcagggca gatgactggg gtgaagtc 1498
Claims (8)
1. Lactobacillus acidophilus LA1063 (A)Lactobacillus acidophilus) It is deposited in China general microbiological culture Collection center with the preservation number of CGMCC number 18744.
2. The lactobacillus acidophilus LA1063 according to claim 1, characterized in that the 16S rDNA sequence of said lactobacillus acidophilus LA1063 has the sequence structure shown in SEQ ID No. 1.
3. A talcum powder using lactobacillus acidophilus LA1063 according to claim 1 or 2, characterized by comprising, in mass percentages: 95-99% of starch and 1-5% of ultramicro-bacterial powder; wherein the submicron bacterium powder comprises 89.5-94.5% of Lactobacillus acidophilus LA1063 powder, 5-10% of microcrystalline cellulose and 0.5-1% of magnesium stearate.
4. The powder of claim 3 wherein the powder is suitable for application to intact skin.
5. A method of making a talcum powder according to claim 3, comprising the steps of:
1) primary mixing: mixing the lactobacillus acidophilus LA1063 powder, the microcrystalline cellulose and the magnesium stearate in the proportion to obtain a bacterium powder mixture;
2) crushing: the bacterial powder mixture is crushed by supersonic airflow and passes through a sieve with the mesh number of 300-500 meshes to form submicron bacterial powder;
3) and (3) secondary mixing: and uniformly mixing the starch and the submicron bacterium powder in the proportion to obtain the cool raw powder.
6. The method according to claim 5, wherein the viable count of the submicron bacterial powder in the step 2) is 1X 1010～1×1011cfu/g。
7. The method of claim 5, wherein the mixture of fungal powder is frozen at-20 ℃ for a period of time greater than 80min prior to step 2).
8. The method of claim 5, wherein the dusting powder is filled after step 3).
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641014A (en) * | 2004-01-18 | 2005-07-20 | 北京三元食品股份有限公司 | Acidophilic lactobacillus and its use |
| CN102204679A (en) * | 2011-06-16 | 2011-10-05 | 高杰 | Quadruple bacterial tablets and preparation method thereof |
| CN104095180A (en) * | 2013-04-12 | 2014-10-15 | 中国科学院烟台海岸带研究所 | Probiotics inulin sheet and preparation method thereof |
| RU2014113474A (en) * | 2014-04-08 | 2015-10-20 | Общество с ограниченной ответственностью "Витбиомед" | MEANS FOR TREATMENT OF INTESTINAL INFECTIONS AND CONDITIONS CAUSED BY BIOBALANCE A DYSBACTERIOSIS |
| CN110384650A (en) * | 2019-08-29 | 2019-10-29 | 湖北省麦诗特生物科技有限公司 | It is a kind of for skin moisture-keeping, the probiotic composition of barrier protection and its application in cosmetics |
-
2019
- 2019-12-25 CN CN201911352405.0A patent/CN113025513A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1641014A (en) * | 2004-01-18 | 2005-07-20 | 北京三元食品股份有限公司 | Acidophilic lactobacillus and its use |
| CN102204679A (en) * | 2011-06-16 | 2011-10-05 | 高杰 | Quadruple bacterial tablets and preparation method thereof |
| CN104095180A (en) * | 2013-04-12 | 2014-10-15 | 中国科学院烟台海岸带研究所 | Probiotics inulin sheet and preparation method thereof |
| RU2014113474A (en) * | 2014-04-08 | 2015-10-20 | Общество с ограниченной ответственностью "Витбиомед" | MEANS FOR TREATMENT OF INTESTINAL INFECTIONS AND CONDITIONS CAUSED BY BIOBALANCE A DYSBACTERIOSIS |
| CN110384650A (en) * | 2019-08-29 | 2019-10-29 | 湖北省麦诗特生物科技有限公司 | It is a kind of for skin moisture-keeping, the probiotic composition of barrier protection and its application in cosmetics |
Non-Patent Citations (3)
| Title |
|---|
| MANESH KUMAR等: ""Antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus against multidrug-resistant enteroaggregative Escherichia coli"", 《INT J ANTIMICROB AGENTS》, vol. 48, no. 3, pages 265 - 270 * |
| 代海兵等: ""嗜酸乳杆菌活菌制剂保护剂及其工艺的研究"", 《中国微生态学杂志》, vol. 24, no. 09, pages 843 - 849 * |
| 郭欣等: ""嗜酸乳杆菌及其在医学上的应用研究进展"", 《内蒙古医学杂志》, vol. 40, no. 09, pages 1097 * |
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