CN113004971A - Method for preparing functional powdered oil by combining germination regulation and enzyme method - Google Patents
Method for preparing functional powdered oil by combining germination regulation and enzyme method Download PDFInfo
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- CN113004971A CN113004971A CN202110275142.9A CN202110275142A CN113004971A CN 113004971 A CN113004971 A CN 113004971A CN 202110275142 A CN202110275142 A CN 202110275142A CN 113004971 A CN113004971 A CN 113004971A
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- 238000000034 method Methods 0.000 title claims abstract description 48
- 230000035784 germination Effects 0.000 title claims abstract description 26
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 21
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000001963 growth medium Substances 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000032683 aging Effects 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 230000007226 seed germination Effects 0.000 claims abstract description 11
- 229920001817 Agar Polymers 0.000 claims abstract description 8
- 239000008272 agar Substances 0.000 claims abstract description 8
- 238000000926 separation method Methods 0.000 claims abstract description 8
- 239000003381 stabilizer Substances 0.000 claims abstract description 8
- 239000008223 sterile water Substances 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 6
- 230000004151 fermentation Effects 0.000 claims abstract description 6
- 238000000227 grinding Methods 0.000 claims abstract description 4
- 238000000643 oven drying Methods 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 6
- 230000003197 catalytic effect Effects 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000012510 hollow fiber Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 239000006227 byproduct Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000003921 oil Substances 0.000 description 32
- 235000019198 oils Nutrition 0.000 description 32
- 239000000341 volatile oil Substances 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention discloses a method for preparing functional powdered oil by combining germination regulation and enzyme method, which comprises the following steps: the method comprises the following steps: selecting seeds, namely selecting full and glossy seeds which are harvested each year, and performing the second step: seed germination treatment, namely cleaning the seeds selected in the step one by using sterile water, and putting the seeds into a germination chamber; crushing and stir-frying, and step four: and (3) oil culture, namely putting the crushed and stir-fried seed grains into a culture medium, then proportioning a certain amount of glucose, agar, a stabilizer and water, and then placing the culture medium into a sterilization box for sterilization, wherein the fourth step is that: aging treatment, step five: separation and extraction: collecting aged thallus, oven drying, grinding, extracting with ethanol for 8 hr, distilling to remove ethanol, and filtering to obtain functional powdered oil. The method has the advantages of strong operability, high extraction efficiency, low cost, simple operation and easy industrial transportation, effectively reduces the generation of byproducts in the seed fermentation process, and improves the utilization rate of raw materials.
Description
Technical Field
The invention belongs to the technical field of oil production methods, and particularly relates to a method and a device for preparing functional powdered oil by combining germination regulation and enzyme method.
Background
The essential oil is an oily substance with characteristic aroma and capable of being distilled out from a plant body along with steam, and consists of a plurality of small molecular compounds, such as aldehydes, alcohols, phenols, esters, terpenes, aromatic hydrocarbons and the like, and has biological activities of oxidation resistance, bacteriostasis, inflammation diminishing, cancer resistance and the like, but the effective application of the essential oil in the fields of food, daily chemicals and the like is limited by the properties of easy volatilization, easy oxidation, difficult water dissolution and the like and odor factors, so the extraction research on the vegetable oil is very important.
For example, patent application No. 201511001696.0 discloses a method for preparing a novel functional vegetable powdered oil, comprising the following steps: (1) extracting and purifying oil bodies from plant seeds; (2) embedding plant essential oil by using the oil body in the step (1), and purifying and concentrating the obtained oil body embedded object by adopting an ultrafiltration technology; (3) and (3) carrying out spray drying on the oil body embedded matter subjected to ultrafiltration purification and concentration in the step (2) to prepare powdered oil.
When the method is used, the oil in the seeds is difficult to extract fully, the separation is not thorough, and the preparation quality of the functional powdered oil is difficult to ensure, so that the research on a method for preparing the functional powdered oil by combining germination regulation and enzyme method is necessary.
Disclosure of Invention
The present invention has been made to solve the above-mentioned problems occurring in the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for preparing functional powdered oil by combining germination regulation and enzyme method comprises the following specific steps:
the method comprises the following steps: selecting seeds, namely selecting full and glossy seeds which are harvested each year, removing suspended seeds by adopting a water floating method, and then drying the seeds to ensure that the relative humidity of the seeds is 3-5% for later use;
step two: seed germination treatment, namely cleaning the seeds selected in the step one by using sterile water, putting the seeds into a germination chamber, adjusting the temperature of the germination chamber to be between 40 and 50 ℃ and the humidity to be between 30 and 50 percent according to the requirement of seed germination, and injecting a certain amount of catalytic enzyme;
step three; crushing and stir-frying, namely drying the germinated seeds in the step two to ensure that the water content in the seeds is between 5 and 7 percent, then putting the seeds into a crusher for crushing, and putting crushed seeds into a stir-frying pan for stir-frying;
step four: culturing oil; putting the crushed and stir-fried seed grains into a culture medium, then proportioning a certain amount of glucose, agar, a stabilizer and water, then placing the culture medium into a sterilization box for sterilization, inoculating a strain after the culture medium is cooled, culturing for 5 days at 26 ℃, inoculating hypha into a shake flask for seed activation culture, and then shaking the culture medium for continuous culture for 1-3 days.
Step four: and (3) aging, namely aging the fermentation liquor containing a large amount of bacteria after culturing for 6-8 days at a constant temperature.
Step five: separation and extraction: collecting aged thallus, oven drying, grinding, extracting with ethanol for 8 hr, distilling to remove ethanol, and filtering to obtain functional powdered oil.
As a further scheme of the invention, the seeds after seed selection are placed in a sealed glass jar.
As a further scheme of the invention, sterile water treatment is carried out for multiple times of cleaning in the seed germination treatment, and the cleaned seeds are also washed by clear water.
As a further scheme of the invention, the size of the seeds crushed by the crusher in the third step is between 3mm and 5 mm.
As a further scheme of the invention, the mixture ratio of each component in the oil culture is 60 parts of seed particles, 10 parts of glucose, 10 parts of agar, 0.2 part of stabilizer and 80 parts of water, and the strain is ME-AA01 strain.
As a further embodiment of the present invention, the shaking flask in the fourth step is premised on: the culture medium comprises the following substances in mass-to-volume ratio: 4-6% of glucose, 0.3-0.6% of yeast extract, KH2PO40.3-0.5% of NaNO30.2-0.5%, the rotation speed of the shake flask is 120-180 rpm, and the temperature is 25-28 ℃.
As a further scheme of the invention, the constant temperature in the aging treatment process is 25-28 ℃.
As a further embodiment of the invention, the medium is not aerated or is kept aerated during the aging treatment, and a carbon source and a nitrogen source are added during the aging treatment.
As a further scheme of the present invention, the membrane material used for the filtration in the separation and extraction is an organic membrane, an inorganic membrane or an organic and inorganic composite membrane; the membrane module is in a hollow fiber type, a sheet type or a tubular type.
Compared with the prior art, the invention has the beneficial effects that:
1. the method has the advantages of strong operability, high extraction efficiency, low cost, simple operation and easy industrial transportation, effectively reduces the generation of byproducts in the seed fermentation process, and improves the utilization rate of raw materials;
2. according to the method, the catalytic enzyme is adopted to promote the germination of the seeds, so that the internal structure of the seeds is quickly and stably realized, and a certain guarantee is provided for the subsequent full extraction of the oil;
3. the invention utilizes ultrafiltration technology to purify and enrich the extracted novel oil body, removes the unextracted essential oil component and the unsaturated fatty acid component released by oil body fracture, and well ensures the extraction quality of functional powdered oil;
drawings
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.
FIG. 1 is a block diagram of a method for preparing functional powdered oil by combining germination control with an enzymatic method.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
Referring to fig. 1, in the embodiment of the present invention, a method for preparing functional powdered oil by combining germination regulation and enzyme method includes the specific steps:
the method comprises the following steps: selecting seeds, namely selecting full and glossy seeds which are harvested each year, removing suspended seeds by adopting a water floating method, and then drying the seeds to ensure that the relative humidity of the seeds is 3-5% for later use;
step two: seed germination treatment, namely cleaning the seeds selected in the step one by using sterile water, putting the seeds into a germination chamber, adjusting the temperature of the germination chamber to be between 40 and 50 ℃ and the humidity to be between 30 and 50 percent according to the requirement of seed germination, and injecting a certain amount of catalytic enzyme;
step three; crushing and stir-frying, namely drying the germinated seeds in the step two to ensure that the water content in the seeds is between 5 and 7 percent, then putting the seeds into a crusher for crushing, and putting crushed seeds into a stir-frying pan for stir-frying;
step four: culturing oil; putting the crushed and stir-fried seed grains into a culture medium, then proportioning a certain amount of glucose, agar, a stabilizer and water, then placing the culture medium into a sterilization box for sterilization, inoculating a strain after the culture medium is cooled, culturing for 5 days at 26 ℃, inoculating hypha into a shake flask for seed activation culture, and then shaking the culture medium for continuous culture for 1-3 days.
Step four: and (3) aging, namely aging the fermentation liquor containing a large amount of bacteria after culturing for 6-8 days at a constant temperature.
Step five: separation and extraction: collecting aged thallus, oven drying, grinding, extracting with ethanol for 8 hr, distilling to remove ethanol, and filtering to obtain functional powdered oil.
Example two
As shown in fig. 1, this embodiment is substantially the same as embodiment 1, and preferably, the seeds after seed selection are placed in a sealed glass jar.
The catalytic enzyme is adopted to promote the germination of the seeds, so that the internal structure of the seeds is quickly and stably realized, and a certain guarantee is provided for the subsequent full extraction of the grease.
EXAMPLE III
As shown in FIG. 1, this embodiment is substantially the same as embodiment 1, and preferably, the sterile water treatment in the seed germination treatment is washed for a plurality of times, and the washed seeds are also washed with clear water.
In the embodiment, sterile water treatment is carried out for multiple times of cleaning in seed germination treatment, so that mould on the surface of seeds is fully removed, and the quality of the seeds for converting oil is greatly improved.
Example four
As shown in fig. 1, this embodiment is substantially the same as embodiment 1, and preferably, the size of the seeds crushed by the crusher in the third step is between 3mm and 5 mm.
In the embodiment, the size of the seeds crushed by the crusher is 3-5 mm, so that the seeds are fully contacted with reactants, and the oil conversion efficiency of the seeds is greatly improved.
EXAMPLE five
Referring to FIG. 1, the present embodiment is substantially the same as example 1, and preferably, the ratio of the components in the oil culture is 60 parts of seed particles, 10 parts of glucose, 10 parts of agar, 0.2 part of stabilizer and 80 parts of water, and the strain is ME-AA01 strain.
The bottle shaking in the fourth step is based on the premise that: the culture medium comprises the following substances in mass-to-volume ratio: 4-6% of glucose, 0.3-0.6% of yeast extract, KH2PO40.3-0.5% of NaNO30.2-0.5%, the rotation speed of the shake flask is 120-180 rpm, and the temperature is 25-28 ℃.
In the embodiment, the mixture ratio of each component in the oil culture is 60 parts of seed particles, 10 parts of glucose, 10 parts of agar, 0.2 part of stabilizing agent and 80 parts of water, so that the medium-division fermentation and transformation of the seeds are realized.
EXAMPLE six
As shown in fig. 1, this embodiment is substantially the same as embodiment 1, and preferably, the constant temperature during the aging treatment is 25 to 28 ℃.
In the aging treatment process, the culture medium is not aerated or is kept aerated, and a carbon source and a nitrogen source are added during aging.
In the embodiment, the treatment is carried out through constant pressure, and the culture medium is not aerated or is kept aerated and a carbon source and a nitrogen source are added during the treatment, so that the oil and fat of the seeds are more completely transformed.
EXAMPLE seven
As shown in fig. 1, this embodiment is substantially the same as embodiment 1, and preferably, the membrane material used for the filtration in the separation and extraction is an organic membrane, an inorganic membrane or an organic and inorganic composite membrane; the membrane module is in a hollow fiber type, a sheet type or a tubular type.
In the embodiment, the novel extracted oil body is purified and enriched by using an ultrafiltration technology, so that the non-extracted essential oil components and unsaturated fatty acid components released by oil body fracture are removed, and the quality of the functional powdered oil extraction is well ensured.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (9)
1. A method for preparing functional powdered oil by combining germination regulation and enzyme method is characterized by comprising the following specific steps:
the method comprises the following steps: selecting seeds, namely selecting full and glossy seeds which are harvested each year, removing suspended seeds by adopting a water floating method, and then drying the seeds to ensure that the relative humidity of the seeds is 3-5% for later use;
step two: seed germination treatment, namely cleaning the seeds selected in the step one by using sterile water, putting the seeds into a germination chamber, adjusting the temperature of the germination chamber to be between 40 and 50 ℃ and the humidity to be between 30 and 50 percent according to the requirement of seed germination, and injecting a certain amount of catalytic enzyme;
step three; crushing and stir-frying, namely drying the germinated seeds in the step two to ensure that the water content in the seeds is between 5 and 7 percent, then putting the seeds into a crusher for crushing, and putting crushed seeds into a stir-frying pan for stir-frying;
step four: culturing oil; putting the crushed and stir-fried seed grains into a culture medium, then proportioning a certain amount of glucose, agar, a stabilizer and water, then placing the culture medium into a sterilization box for sterilization, inoculating a strain after the culture medium is cooled, culturing for 5 days at 26 ℃, inoculating hypha into a shake flask for seed activation culture, and then shaking the culture medium for continuous culture for 1-3 days.
Step four: and (3) aging, namely aging the fermentation liquor containing a large amount of bacteria after culturing for 6-8 days at a constant temperature.
Step five: separation and extraction: collecting aged thallus, oven drying, grinding, extracting with ethanol for 8 hr, distilling to remove ethanol, and filtering to obtain functional powdered oil.
2. The method for preparing functional powdered oil and fat by using germination regulation and enzyme method according to claim 1, wherein the seeds after seed selection are placed in a sealed glass jar.
3. The method for preparing functional powdered oil by combining germination regulation and enzyme method according to claim 1, wherein sterile water treatment in seed germination treatment is performed for multiple times of cleaning, and the cleaned seeds are washed by clear water.
4. The method for preparing functional powdered oil and fat by combining germination regulation and enzyme method according to claim 1, wherein the size of the seeds crushed by the crusher in the third step is 3-5 mm.
5. The method for preparing functional powdered oil by combining germination regulation and enzyme method according to claim 1, wherein the ratio of each component in oil culture is 60 parts of seed particles, 10 parts of glucose, 10 parts of agar, 0.2 part of stabilizer and 80 parts of water, and the strain is ME-AA01 strain.
6. The method for preparing functional powdered oil by combining germination regulation and enzyme method according to claim 1, wherein the shaking flask precondition in the four steps is that: the culture medium comprises the following substances in mass-to-volume ratio: 4-6% of glucose, 0.3-0.6% of yeast extract, KH2PO40.3-0.5% of NaNO30.2-0.5%, the rotation speed of the shake flask is 120-180 rpm, and the temperature is 25-28 ℃.
7. The method for preparing functional powdered oil by combining germination regulation and enzyme method according to claim 1, wherein the constant temperature in the aging treatment process is 25-28 ℃.
8. The method for preparing functional powdered oil and fat by using germination regulation and enzyme combination method according to claim 1, wherein the culture medium is not aerated or is kept aerated during the aging treatment, and a carbon source and a nitrogen source are added during the aging treatment.
9. The method for preparing functional powdered oil by combining germination regulation and enzyme method according to claim 1, wherein the membrane material used for filtration in the separation and extraction is organic membrane, inorganic membrane or organic and inorganic composite membrane; the membrane module is in a hollow fiber type, a sheet type or a tubular type.
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|---|---|---|---|---|
| CN101109015A (en) * | 2007-07-09 | 2008-01-23 | 南京工业大学 | Preparation method of arachidonic acid grease |
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| JP2018033433A (en) * | 2016-09-02 | 2018-03-08 | ミヨシ油脂株式会社 | Strain belonging to mortierella, and oils and fats production method |
| WO2018120574A1 (en) * | 2016-12-30 | 2018-07-05 | 内蒙古金达威药业有限公司 | Method for producing dha by microbial fermentation |
| US20200332220A1 (en) * | 2018-12-21 | 2020-10-22 | Jiangnan University | Method for Preparing Functional Grease through Segmented Solid-State Fermentation of Mixed Fungi |
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2021
- 2021-03-15 CN CN202110275142.9A patent/CN113004971A/en active Pending
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| CN101109015A (en) * | 2007-07-09 | 2008-01-23 | 南京工业大学 | Preparation method of arachidonic acid grease |
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| US20110065940A1 (en) * | 2009-06-24 | 2011-03-17 | Neste Oil Oyj | Method for the production of fat |
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| WO2018120574A1 (en) * | 2016-12-30 | 2018-07-05 | 内蒙古金达威药业有限公司 | Method for producing dha by microbial fermentation |
| US20200332220A1 (en) * | 2018-12-21 | 2020-10-22 | Jiangnan University | Method for Preparing Functional Grease through Segmented Solid-State Fermentation of Mixed Fungi |
Non-Patent Citations (1)
| Title |
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Application publication date: 20210622 |