CN112986577B - Kit for detecting a substance in a sample - Google Patents

Kit for detecting a substance in a sample Download PDF

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Publication number
CN112986577B
CN112986577B CN202110150234.4A CN202110150234A CN112986577B CN 112986577 B CN112986577 B CN 112986577B CN 202110150234 A CN202110150234 A CN 202110150234A CN 112986577 B CN112986577 B CN 112986577B
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China
Prior art keywords
reagent
bin
gasket
inner cylinder
lug
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Active
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CN202110150234.4A
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CN112986577A (en
Inventor
凌健东
许爱红
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Shenzhen Haisian Biotechnology Co ltd
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Shenzhen Haisian Biotechnology Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Rehabilitation Therapy (AREA)
  • Rheumatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a kit, which comprises an outer cylinder, an inner cylinder and a gasket, wherein an accommodating groove is formed in the outer cylinder, a reaction bin is formed at the bottom of the accommodating groove, a filter membrane is arranged in the reaction bin, and a bulge positioned at the periphery of the reaction bin is formed at the bottom of the accommodating groove; the inner barrel is provided with a sample adding chamber, a first reagent bin and a second reagent bin, the first reagent bin and the second reagent bin are positioned at the periphery of the sample adding chamber, the bottoms of the first reagent bin and the second reagent bin are respectively and correspondingly provided with a film, and the first reagent and the second reagent are respectively and correspondingly packaged in the first reagent bin and the second reagent bin; when the inner cylinder stretches into the accommodating groove, the film can be punctured by the bulge; the gasket is arranged between the inner cylinder and the outer cylinder, and when the gasket is arranged between the inner cylinder and the outer cylinder, the film is abutted with the bulge or keeps a distance; when the gasket is removed from between the outer cylinder and the inner cylinder, the inner cylinder extends into the outer cylinder, and the membrane can be pierced by the protrusions. The technical scheme of the invention ensures that the detection of the protein content in blood is simpler.

Description

Kit for detecting a substance in a sample
Technical Field
The invention relates to the technical field of biology, in particular to a kit.
Background
For quantitative determination of protein content in blood, the steps are very complicated, time is consumed, a large amount of manpower is consumed, and the whole operation process is very inconveniently.
In the case of measuring the protein content in blood, a common liquid phase detection kit is generally used as a kit for packaging multiple bottles of reagents, and more consumables such as an EP tube, a sample adding gun head, a sample adding tube and the like are consumed in the use process.
Disclosure of Invention
The invention mainly aims to provide a kit for solving the problem that the existing measuring mode is not concise.
To achieve the above object, the present invention provides a kit comprising:
the device comprises an outer barrel, wherein a containing groove is formed in the outer barrel, a reaction bin is formed at the bottom of the containing groove, a filter membrane is arranged in the reaction bin, and a protrusion positioned at the periphery of the reaction bin is formed at the bottom of the containing groove;
the inner barrel is provided with a sample adding chamber, a first reagent bin and a second reagent bin, the first reagent bin and the second reagent bin are positioned at the periphery of the sample adding chamber, the bottoms of the first reagent bin and the second reagent bin are respectively and correspondingly provided with a film, and the first reagent bin and the second reagent bin are respectively and correspondingly packaged with a first reagent and a second reagent; when the inner cylinder stretches into the accommodating groove, the film can be punctured by the bulge;
a gasket for mounting between the inner cylinder and the outer cylinder, the membrane abutting or maintaining a gap with the protrusion when the gasket is disposed between the inner cylinder and the outer cylinder; when the gasket is removed from between the outer barrel and the inner barrel, the inner barrel extends into the outer barrel, and the membrane is penetrable by the protrusions.
In an embodiment, the lateral wall of the outer cylinder is laterally protruded with a first lug, the lateral wall of the inner cylinder is laterally protruded with a second lug, and when the inner cylinder stretches into the outer cylinder, the gasket is used for being sleeved on the periphery of the inner cylinder so as to separate the first lug from the second lug.
In one embodiment, the side walls of the reaction chamber are at least partially transparent.
In one embodiment, the first reagent cartridge and the second reagent cartridge are symmetrically disposed with respect to the loading chamber.
In an embodiment, the inner cylinder is adapted to the accommodating groove, and the outer contour of the cross section of the inner cylinder and the inner contour of the cross section of the outer cylinder are all in non-circular arrangement.
In one embodiment, the free end of the protrusion is pointed.
In an embodiment, after the gasket is removed from between the outer cylinder and the inner cylinder, and when the first lug is abutted with the second lug, a gap is formed between the bottom of the inner cylinder and the bottom of the accommodating groove.
In an embodiment, a nozzle communicated with the sample adding chamber is formed at the bottom of the inner cylinder, and when the gasket is abutted with the first lug and the second lug, the nozzle stretches into the reaction chamber, and a gap is formed between the nozzle and the bottom of the reaction chamber.
In an embodiment, the surface of the gasket is provided with a flexible layer and/or the surface of the gasket is provided with an adhesive layer.
In one embodiment, the gasket is a hard piece.
The technical scheme of the invention ensures that the detection of the protein content in blood is simpler.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an exploded view of a kit of the present invention;
FIG. 2 is a schematic view of the assembled cartridge of FIG. 1;
FIG. 3 is a cross-sectional view of FIG. 2;
FIG. 4 is a schematic view of the inner barrel of FIG. 1;
FIG. 5 is a cross-sectional view of the inner barrel of FIG. 4;
FIG. 6 is a schematic view of the outer barrel of FIG. 1;
FIG. 7 is a cross-sectional view of FIG. 6;
FIG. 8 is a schematic view of the inner and outer barrel assembly of FIG. 1 with the gasket removed;
fig. 9 is a cross-sectional view of fig. 8.
Reference numerals illustrate:
reference numerals Name of the name Reference numerals Name of the name
10 Kit for detecting a substance in a sample 11 Outer cylinder 11
12 Inner cylinder 13 Gasket ring
14 Filter membrane 15 Protrusions
16 Film and method for producing the same 11a First lug
12a Second lug 12b Barrel nozzle
111 Accommodating groove 112 Tank bottom
113 Reaction bin 114 Warehouse bottom
120 Sample adding chamber 121 First reagent chamber
122 Second reagent chamber 123 Bottom surface of inner cylinder
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
Furthermore, the description of "first," "second," etc. in this disclosure is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
Referring to fig. 1 and 2, the present invention provides a kit 10, and the kit 10 includes an outer cylinder 11, an inner cylinder 12 and a gasket 13.
Referring to fig. 3, 6 and 7, the outer cylinder 11 has a receiving groove 111 formed therein, a reaction chamber 113 is formed at a bottom 112 of the receiving groove 111, and a protrusion 15 is formed at a periphery of the reaction chamber 113 at the bottom 112 of the receiving groove 111.
Referring to fig. 3, 4 and 5, the inner cylinder 12 has a loading chamber 120, a first reagent chamber 121 and a second reagent chamber 122, the first reagent chamber 121 and the second reagent chamber 122 are located at the periphery of the loading chamber 120, and the inner cylinder 12 is configured to extend into the accommodating groove 111, so that the first reagent chamber 121 and the second reagent chamber 122 can respectively correspond to the positions of the protrusions 15 for inserting the protrusions 15.
With continued reference to fig. 3 and 9, a washer 13 is provided between the inner barrel 12 and the outer barrel 11 for adjusting the depth of the outer barrel 11 extending into the inner barrel 12.
Specifically, the kit 10 is used for rapid detection of antigen or antibody proteins in an immune response, such as anti-syphilis antibodies, dengue antigens, anti-dsDNA antibodies, anti-toxoplasma antibodies, and the like. In the kit 10, in the actual test, a filter membrane 14 is required to be disposed in the reaction chamber 113, and the filter membrane 14 is used for filtering blood cells, fibrin and the like in blood, so that the blood cells, the fibrin and the like remain on the upper side of the filter membrane 14, and serum passes through the filter membrane 14 and enters the reaction chamber 113.
The first reagent compartment 121 and the second reagent compartment 122 respectively encapsulate a first reagent and a second reagent, wherein the first reagent is a magnetic particle solution labeled with an antigen or an antibody, and the second reagent is a color latex microsphere solution labeled with an antibody or an antigen. The bottoms of the first reagent chamber 121 and the second reagent chamber 122 are respectively provided with a film 16, and when the inner cylinder 12 stretches into the accommodating groove 111, the films 16 can be pierced by the protrusions 15, so that the first reagent and the second reagent flow into the reaction chamber 113 in sequence.
The gasket 13 is used for adjusting the depth of the inner cylinder 12 extending into the outer cylinder 11, and when the gasket 13 is arranged between the inner cylinder 12 and the outer cylinder 11, the thin film 16 is abutted with or kept at a distance from the bulge 15; when the washer 13 is removed from between the outer cylinder 11 and the inner cylinder 12, the inner cylinder 12 extends into the outer cylinder 11, and the membrane 16 is pierceable by the projection 15 (the free end of the projection 15 is pointed in order to make it easier for the projection 15 to pierce the membrane 16).
The measurement process is as follows:
first: the gasket 13 is fitted around the outer periphery of the inner tube 12, and the inner tube 12 is inserted into the outer tube 11 (in this case, the film 16 is not pierced by the protrusions 15 under the action of the gasket 13).
Second,: a blood sample is added to the loading chamber 120, and the blood drops down through the loading chamber 120 onto the filter membrane 14, is filtered through the filter membrane 14, and the serum passes through the filter membrane 14 and flows into the bottom of the reaction chamber 113.
Third,: pulling the inner cylinder 12 out of the outer cylinder 11, removing the gasket 13, then inserting the inner cylinder 12 into the outer cylinder 11 continuously, and pressing the inner cylinder 12 to make the bulge 15 puncture the membrane 16 at the bottom of the first reagent chamber 121, so that the first reagent can flow into the reaction chamber 113 (the filter membrane 14 is used for filtering blood cells, fibrin and the like, and does not have a filtering effect on the first reagent); the kit 10 is gently shaken to allow sufficient contact and reaction between the first reagent and serum.
Fourth,: the inner cylinder 12 is pulled out of the outer cylinder 11, rotated by a preset angle (which may be 90 ° or 180 °, since the error in operation is small for a better operation of 90 ° and 180 °), then the inner cylinder 12 is continuously inserted into the outer cylinder 11, and the inner cylinder 12 is pressurized so that the projections 15 puncture the membrane 16 at the bottom of the second reagent cartridge 122, whereby the second reagent can flow into the reaction cartridge 113. The kit 10 is vigorously shaken to allow the second reagent to react well with the solution in the third step.
Fifth,: after the reaction of the serum, the first reagent and the second reagent is completed, a magnetic field is applied to the kit 10, and at this time, magnetic particles are deposited at the bottom of the reaction chamber 113.
Sixth: the color value of the supernatant in the reaction chamber 113 is read by a colorimetric card, and the level of the substance to be detected is calculated.
The kit includes 2 solutions, one is a magnetic particle solution labeled with an antigen or an antibody, and the other is a color latex microsphere solution labeled with an antibody or an antigen. When detecting, the substance to be detected is firstly combined with the magnetic particles marked with the antigen (antibody) to form a magnetic particle-antigen (antibody) -substance to be detected-compound, after the reaction, the color microsphere marked with the antibody (antigen) is added, under the action of an external magnetic attraction device, the color microsphere marked with the antibody (antigen) is combined with the compound to precipitate along with the magnetic particles, and the unbound microsphere is remained in the supernatant. The color in the supernatant is in a certain proportion to the level of the substance to be detected. And reading the color value through the colorimetric card, and calculating the level of the substance to be detected.
The following examples of anti-Cyclic Citrullinated Peptide (CCP) antibody detection reagents illustrate the reagent test.
Magnetic microparticles label Cyclic Citrullinated Peptide (CCP) antigen and colored microspheres label CCP antigen-specific antibodies. CCP antibodies in the sample compete with the color microsphere-labeled antibodies for the magnetic particle-labeled antigen at the same time. During detection, a sample to be detected is firstly added for reaction for 2min, CCP antibodies in the sample are combined with CCP antigens on the magnetic particles, then color microspheres marked with specific antibodies are added, the CCP antibodies compete for combining with the antigens on the magnetic particles, after magnetic separation, the color microspheres marked with the antibodies combined with the antigens on the magnetic particles are precipitated along with the magnetic particles, and unbound color microspheres are remained in the supernatant. And comparing the color in the supernatant with the color comparison card, wherein the color score in the supernatant is in a direct proportion relation with the anti-CCP antibody in the sample, namely the higher the score is, the higher the concentration of the CCP antibody to be detected is.
The kit 10 of the invention has simple detection flow and can rapidly acquire detection results when measuring a sample to be detected.
In an embodiment, referring to fig. 6 and 7, in order to facilitate the installation of the gasket 13, a first lug 11a protrudes laterally from the side wall of the outer cylinder 11, a second lug 12a protrudes laterally from the side wall of the inner cylinder 12, and when the inner cylinder 12 extends into the outer cylinder 11, the gasket 13 is used to be sleeved on the outer periphery of the inner cylinder 12 so as to block the first lug 11a from the second lug 12 a.
Here, referring to fig. 3, when the gasket 13 is placed between the first lug 11a and the second lug 12a, the depth of the inner cylinder 12 extending into the outer cylinder 11 is reduced, so that the film 16 of the first reagent cartridge 121 or the second reagent cartridge 122 can be effectively prevented from being pierced by the protrusions 15. Referring to fig. 8 and 9, when the gasket 13 is withdrawn from between the first lug 11a and the second lug 12a, after the inner cylinder 12 is inserted into the outer cylinder 11, the first lug 11a abuts against the second lug 12a, and the membrane 16 is pierced by the protrusion 15, so that the first reagent and the second reagent can flow into the reaction chamber 113. Here, it is considered that direct abutment of the groove bottom 112 of the accommodation groove 111 with the bottom of the inner tube 12 may affect the flow of the first reagent and the second reagent. In an embodiment, after the gasket 13 is removed from between the outer cylinder 11 and the inner cylinder 12, and when the first lug 11a abuts against the second lug 12a, a gap is formed between the bottom of the inner cylinder 12 and the bottom 112 of the accommodating groove 111. In the case where the gap exists, the first reagent (or the second reagent) can smoothly flow into the reaction chamber 113 after the membrane 16 is pierced by the projections 15.
The bottom of the reaction chamber 113 includes a chamber bottom 114 of the reaction chamber 113 and a side wall portion adjacent to the chamber bottom 114. Considering that the complex formed after the reaction of the serum, the first reagent and the second reagent is precipitated at the bottom of the reaction chamber 113, in order to facilitate the comparison of the color of the supernatant, in principle, it is only necessary that the side wall of the reaction chamber 113 is left with a viewing area (transparent), i.e. the side wall of the reaction chamber 113 is at least partially transparent. For example, the entire reaction chamber 113 is transparent, or one side wall of the reaction chamber 113 is transparent, or the opposite or adjacent two side walls of the reaction chamber 113 are transparent. The bottom 114 of the reaction chamber 113 may also be made transparent (so that precipitation of the antibody-labeled colored microspheres with magnetic particles can be visually observed), although the opacity has no effect.
In a preferred embodiment, all of the sidewalls of the reaction chamber 113 are preferably transparent. For example, the side wall of the reaction chamber 113 is glass or transparent plastic.
In one implementation, referring to fig. 5, in order to facilitate the switching between the first reagent chamber 121 and the second reagent chamber 122, the first reagent chamber 121 and the second reagent chamber 122 are symmetrically disposed with respect to the sample addition chamber 120 (corresponding to the case that the user switches between the first reagent chamber 121 and the second reagent chamber 122, only the inner cylinder 12 needs to be rotated 180 °).
After the membrane 16 of the first reagent chamber 121 is pierced by the protrusion 15 and the first reagent flows into the reaction chamber 113, the inner cylinder 12 needs to be pulled out of the outer cylinder 11 to switch the second reagent chamber 122 to correspond to the protrusion 15, and the user only needs to rotate the inner cylinder 12 by 180 ° because the first reagent chamber 121 and the second reagent chamber 122 are symmetrical.
On the basis of the above embodiment, please continue to refer to fig. 5, when the inner cylinder 12 is inserted into the outer cylinder 11, in order to make the first reagent chamber 121 and the second reagent chamber 122 more accurately correspond to the positions of the protrusions 15, in this embodiment, the inner cylinder 12 is adapted to the accommodating groove 111, and the outer contour of the cross section of the inner cylinder 12 and the inner contour of the cross section of the outer cylinder 11 are all non-circular.
This arrangement of the outer profile of the cross section of the inner barrel 12 and the inner profile of the cross section of the outer barrel 11 prevents mainly relative rotation of the two, and it is difficult to align the projections 15 with the first and second reagent chambers 121, 122 if relative rotation is also possible after insertion of the inner barrel 12 into the outer barrel 11.
The shape of the outer contour of the cross section of the inner cylinder 12 and the inner contour of the cross section of the outer cylinder 11 may be an ellipse, a racetrack shape, a rectangle, a triangle, etc., and in order to enhance the visibility of the inner cylinder 12 and the outer cylinder 11, the shape is preferably an ellipse or a racetrack shape. Because of the oval or racetrack-shaped arrangement of the outer contour of the cross section of the inner barrel 12, there are only two correspondences with the oval or racetrack-shaped arrangement of the inner contour of the cross section of the outer barrel 11, which exactly correspond to the first reagent reservoir 121 with the projection 15 and the second reagent reservoir 122 with the projection 15.
The bottom of the loading chamber 120 of the inner cartridge 12 may be substantially flush with the bottom of the first reagent cartridge 121 or the second reagent cartridge 122, but the flush arrangement has the following drawbacks: after the blood sample is introduced from the loading chamber 120, the blood will wall up and some of the blood will flow to the bottom 123 of the inner barrel 12, and even near the membrane 16, and possibly around the protrusions 15, which will affect the amount of sample to be introduced, while there will be relatively little sample to be introduced, which will ultimately affect the test results.
In view of this, in an embodiment, with continued reference to fig. 5 and 9, a nozzle 12b communicating with the sample application chamber 120 is formed at the bottom of the inner cylinder 12, and when the gasket 13 abuts against the first lug 11a and the second lug 12a, the nozzle 12b extends into the reaction chamber 113, and a gap is provided between the nozzle 12b and the filter membrane 14.
Thus, when blood is fed from the loading chamber 120 and filtered by the filter membrane 14, almost all serum can flow through the filter membrane 14 to the bottom of the reaction chamber 113 without affecting the loading amount.
In another embodiment, referring to fig. 1-3, the gasket 13 may be provided in a variety of forms. For example, the surface of the gasket 13 is provided with a flexible layer, so that the cartridge 10 itself is more stable when the cartridge 10 is measuring a sample (even if it is affected by external vibration, the inner tube 12 does not deviate significantly from the outer tube 11 due to the flexible layer of the gasket 13).
For another example, the surface of the gasket 13 is provided with an adhesive layer, and the adhesive layer may make the first and second lugs 11a and 12a each more firmly fixed to the gasket 13.
For another example, the gasket 13 may also be a hard piece. Because the bottom surface of the first lug 11a and the top surface of the second lug 12a are both plane, the gasket 13 is made of hard materials, so that the fit between the three parts is smoother.
The foregoing description is only of the preferred embodiments of the present invention and is not intended to limit the scope of the invention, and all equivalent structural changes made by the description of the present invention and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the invention.

Claims (6)

1. A kit, comprising:
the device comprises an outer barrel, wherein a containing groove is formed in the outer barrel, a reaction bin is formed at the bottom of the containing groove, a filter membrane is arranged in the reaction bin, and a protrusion positioned at the periphery of the reaction bin is formed at the bottom of the containing groove;
the inner barrel is provided with a sample adding chamber, a first reagent bin and a second reagent bin, the first reagent bin and the second reagent bin are positioned at the periphery of the sample adding chamber, the bottoms of the first reagent bin and the second reagent bin are respectively and correspondingly provided with a film, and the first reagent bin and the second reagent bin are respectively and correspondingly packaged with a first reagent and a second reagent; when the inner cylinder stretches into the accommodating groove, the film can be punctured by the bulge;
a gasket for mounting between the inner cylinder and the outer cylinder, the membrane abutting or maintaining a gap with the protrusion when the gasket is disposed between the inner cylinder and the outer cylinder; when the gasket is removed from between the outer cylinder and the inner cylinder, the inner cylinder extends into the outer cylinder, and the film can be pierced by the protrusions;
the side wall of the outer cylinder is laterally protruded with a first lug, the side wall of the inner cylinder is laterally protruded with a second lug, and when the inner cylinder stretches into the outer cylinder, the gasket is used for being sleeved on the periphery of the inner cylinder so as to separate the first lug from the second lug;
the side wall of the reaction bin is at least partially transparent;
after the gasket is removed from between the outer cylinder and the inner cylinder, and when the first lug is abutted with the second lug, a gap is formed between the bottom of the inner cylinder and the bottom of the accommodating groove;
the bottom of the inner barrel is provided with a barrel mouth communicated with the sample adding chamber, when the gasket is abutted with the first lug and the second lug, the barrel mouth stretches into the reaction bin, and a gap is reserved between the barrel mouth and the bin bottom of the reaction bin.
2. The kit of claim 1, wherein the first reagent cartridge and the second reagent cartridge are disposed symmetrically with respect to the loading chamber.
3. The kit of claim 2, wherein the inner barrel is adapted to the receiving slot, and the outer profile of the cross section of the inner barrel and the inner profile of the cross section of the outer barrel are both non-circular.
4. The kit of claim 1, wherein the free end of the protuberance is pointed.
5. Kit according to claim 1, wherein the surface of the gasket is provided with a flexible layer and/or the surface of the gasket is provided with an adhesive layer.
6. The kit of claim 1, wherein the gasket is a rigid member.
CN202110150234.4A 2021-02-03 2021-02-03 Kit for detecting a substance in a sample Active CN112986577B (en)

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Citations (8)

* Cited by examiner, † Cited by third party
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